The Effect Of Oleic And Linoleic Acids On In Vitro Bovineembryonic Development And Embryo Quality,
2015
TÜBİTAK
The Effect Of Oleic And Linoleic Acids On In Vitro Bovineembryonic Development And Embryo Quality, Tahi̇r Karaşahi̇n, Şevket Arikan
Turkish Journal of Veterinary & Animal Sciences
The present study was aimed at the investigation of the effects of oleic and linoleic acid on the in vitro development of Anatolian native black crossbred bovine embryos. Following the in vitro maturation and fertilization of oocytes, embryonic development stages were monitored using embryo culture medium supplemented with three different doses of oleic and linoleic acid (10, 100, and 1000 µM). Examination of the culture media 48 h after fertilization demonstrated that linoleic acid had no effect on embryo cleavage rates, while oleic acid produced a significant increase in cleavage rates (P < 0.01). It was determined that the highest dose of linoleic acid (1000 µM) and all three doses of oleic acid significantly increased the proportion of cultured oocytes developing to the morula-blastocyst stage (P < 0.001). As a result, while oleic acid significantly increased (P < 0.001) the proportion of embryos developing to the morula-blastocyst stage, linoleic acid had limited effects on embryonic development and quality. Thus, it was concluded that the supplementation of Anatolian native black crossbred bovine embryo cultures with oleic acid may induce positive effects on embryonic development and quality.
Immunohistochemical Distribution Of Platelet-Derived Growth Factor-C And Platelet-Derived Growth Factor Receptor-? In Small Intestine Of Rats Treated With Capsaicin, Buket Bakir, Ebru Karadağ Sari
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to examine the effect of capsaicin administration immunohistochemically on platelet-derived growth factor (PDGF)-C and platelet-derived growth factor receptor (PDGFR)-\alpha release in the small intestine tissues of rats. Rats were divided into three groups: group 1 (subcutaneous drug administration), group 2 (oral drug administration), and group 3 (subcutaneous sham administration). Histometric measurements and counts were performed in order to determine whether there was a difference between groups in terms of height of villi, depth of crypts, and goblet cell count in small intestine. The small intestine was examined by Crossman?s triple staining method and streptavidin-biotin …
Influences Of Flunixin And Tenoxicam On The Pharmacokinetics Of Florfenicol In Lipopolysaccharide-Induced Endotoxemia,
2015
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Influences Of Flunixin And Tenoxicam On The Pharmacokinetics Of Florfenicol In Lipopolysaccharide-Induced Endotoxemia, Feri̇de Koç, Alptuğ Ati̇la, Emre Karakuş, Kami̇l Üney
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to investigate the influences of flunixin (FM) and tenoxicam (TN) on the pharmacokinetics of florfenicol (FF) after coadministration in lipopolysaccharide (LPS)-induced endotoxemic rabbits. Fifteen male rabbits were used in this study. FF (20 mg/kg), FM (2 mg/kg), and TN (1 mg/kg) were coadministered via intravenous injection to the animals. The concentrations of FF were determined by high-performance liquid chromatography with diode-array detection from 0.08 to 12 h in plasma. The plasma concentration-time profile of FF was described using a noncompartmental open model. In this study, terminal half-life, area under the curve, mean residence time, …
The Effects Of Hypoxia On The Expression Of Vascular Endothelialgrowth Factor In Broiler Lung Fibroblasts,
2015
TÜBİTAK
The Effects Of Hypoxia On The Expression Of Vascular Endothelialgrowth Factor In Broiler Lung Fibroblasts, Musa Özgür Özyi̇ği̇t, Müjdat Müfi̇t Kahraman, Ahmet Akkoç
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the effects of hypoxic stresses on vascular endothelial growth factor (VEGF) in cultured lung fibroblasts of meat-type chickens. Isolated and subcultured primary lung fibroblasts were exposed to hypoxia. The presence of VEGF and the effects of hypoxia on cultured lung fibroblasts and in the culture media were evaluated. Immunocytochemistry using streptavidin-biotin-peroxidase method and ELISA using commercially available primary monoclonal antibodies were used for evaluation in both 6-h and 12-h hypoxic fibroblasts. The level of VEGF was slightly higher in the 6-h hypoxic group (1.22 ng/mL) than in the 6-h control group (1.27 …
Determination Of Nutrient Content And Digestibility Characteristics Ofprangos Ferulacea (L.) On Grazing Lands Of Eastern Anatolia, Reşi̇t Aldemi̇r, Nuri̇ye Tuğba Bi̇ngöl, Mehmet Aki̇f Karsli, İsmai̇l Akça
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to compare nutrient content, and in situ protein and organic matter degradation kinetics of Prangos ferulacea (L.) (locally called helis), a naturally growing plant on the top of Mount Artos, with those of alfalfa. P. ferulacea plants used in the study were collected from three different parts of Mount Artos in Van Province over two years. While organic matter content and in vitro organic matter digestibility of P. ferulacea were significantly higher, neutral detergent fiber, acid detergent fiber, and acid detergent insoluble nitrogen contents were significantly lower compared with those of alfalfa (P < 0.01). In general, in situ dry matter, and organic matter and crude protein degradability of P. ferulacea were significantly higher compared with those of alfalfa starting from 6 h of incubation (P < 0.05). Organic matter and crude protein degradability of P. ferulacea was 79.15% and 85.31%, respectively, after 48 h of incubation. By-pass protein content and both organic matter and crude protein degradation rates were similar between samples (P > 0.05). …
In Vitro Gas Production Of Five Rations Of Different Maize Silage And Concentrate Ratios Influenced By Increasing Levels Of Chemically Characterized Extract Of Salix Babylonica, Mona Mohamed Mohamed Yasseen Elghandour, Ahmed Eid Kholif, Adrian Zaragoza Bastida, Dora Luz Pinzón Martínez, Abdelfattah Zeidan Mohamed Salem
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to assess the effect of the chemical substances of Salix babylonica (SB) extract on in vitro rumen fermentation of five mixed rations with different maize silage to concentrate ratios. Fifty-nine compounds were identified in SB extract using the retention time and mass spectral technique. Interactive effects were noted (P < 0.001) for the asymptotic gas production (GP) (b), the rate of production (c), the initial delay before GP begins (L), pH, dry matter digestibility, metabolizable energy (ME), organic matter digestibility (OMD), short chain fatty acids (SCFAs), gas yield at 24 h (GY_{24}), microbial crude protein, and in vitro GP. Both 1.2 and 1.8 mL SB/g DM had the highest (P < 0.05) b and c values. Addition of 1.2 and 1.8 mL SB/g DM linearly improved (P < 0.001) ME, OMD, SCFAs, and GY24. It could be concluded that, based on the highly detected interaction effects between ration types and extract doses for fermentation parameters and GP, the most effective dose of SB varied between incubated total mixed rations. However, the ration of 25% silage and 75% concentrate had the highest nutritive value, especially at doses of 1.2 to 1.8 mL SB/g DM total mixed ration.
Influence Of Dietary Zilpaterol Hydrochloride On Feedlot Performance, Carcass Traits, Chemical Composition Of Longissimus Muscle, And Plasma Metabolites Of Castrated Male Goats, Ali Hatefi, Armin Towhidi, Abolfazl Zali, Saeed Zeinoaldini, Mahdi Ganjkhanlou, Reza Masoudi, Alejandro Plascencia
Turkish Journal of Veterinary & Animal Sciences
To evaluate the influence of zilpaterol hydrochloride (ZH) supplementation on growth performance, carcass traits, chemical characteristics of the longissimus muscle (LM), and plasma metabolites, 16 Mahabadi castrated goats (29.93 ± 1.84 kg) were individually fed a finishing diet without or with ZH supplementation dosed at 0.20 mg/kg BW daily. Zilpaterol was fed for 30 days with 3 days of withdrawal before harvest. Compared with the controls, ZH supplementation decreased dry matter intake and increased total weight gain, average daily gain, and gain for feed. Hot carcass weight, dressing percentage, and LM area were increased, but back fat thickness and kidney-pelvic …
Serotyping And Antibiotic Susceptibility Of Listeria Monocytogenes Isolatedfrom Ready-To-Eat Foods In Samsun, Turkey,
2015
TÜBİTAK
Serotyping And Antibiotic Susceptibility Of Listeria Monocytogenes Isolatedfrom Ready-To-Eat Foods In Samsun, Turkey, Göknur Terzi̇, Ali̇ Gücükoğlu, Özgür Çadirci, Tolga Uyanik, Mustafa Ali̇şarli
Turkish Journal of Veterinary & Animal Sciences
The objective of the present study was to assess the presence of Listeria monocytogenes and to investigate its serotyping and antibiotic resistance in ready-to-eat (RTE) foods. A total of 100 RTE foods, including 25 stuffed mussels, 25 ezme samples (a Turkish-style tomato dip/condiment), 25 fried spiced livers, and 25 mayonnaise-based salads were obtained from retail shops and supermarkets in Samsun, Turkey. Samples were analyzed using the standard procedure EN ISO 11290-1 and isolates of L. monocytogenes were confirmed for the presence of the hemolysin gene (hylA) by polymerase chain reaction. L. monocytogenes was identified in 1 of 25 (4%) mayonnaise-based …
Serological And Molecular Diagnosis Of Paratuberculosis In Dairy Cattle,
2015
TÜBİTAK
Serological And Molecular Diagnosis Of Paratuberculosis In Dairy Cattle, Kadi̇r Semi̇h Gümüşsoy, Tuba İça, Seçi̇l Abay, Fuat Aydin, Harun Hizlisoy
Turkish Journal of Veterinary & Animal Sciences
In this study, the presence of paratuberculosis was investigated by serological and molecular methods in herds of dairy cattle. Blood, milk, and stool samples of 147 cows aged 2 years old or older with chronic diarrhea were collected. A California mastitis test (CMT) was performed on milk samples. Indirect paratuberculosis enzyme-linked immunosorbent assay (ELISA) test was used for serological investigation. Polymerase chain reaction (PCR) was utilized for molecular identification of Mycobacterium avium subsp. paratuberculosis (MAP) from milk and stool samples. Immunomagnetic separation (IMS) method was used for milk and fecal samples. Eighteen (12.24%), 44 (29.93%), 36 (24.49%), 28 (19.05%), and …
Effects Of Dietary Supplementation Of Probiotics (Bacillus Subtilis, Bacillus Licheniformis, And Bacillus Natto) On Broiler Muscle Development And Meat Quality, Xianjian Zhou, Erhui Jin, Shenghe Li, Chenfang Wang, Enmei Qiao, Guozhong Wu
Turkish Journal of Veterinary & Animal Sciences
This paper aims to evaluate the effects of probiotics (Bacillus subtilis, Bacillus licheniformis, and Bacillus natto) on muscle development and meat quality of broilers. In this study, 480 broilers (1 day old) were randomly divided into 4 groups. Group A was fed with a basal diet. The basal diets of Group B, Group C, and Group D were respectively supplemented with 50 mg/kg chlortetracycline, 200 mg/kg probiotics, and 400 mg/kg probiotics. On day 21 and day 42, in each group, 18 randomly selected broilers were tested to determine muscle performances. On day 21, compared with Group A, Group C and …
Identification Of Simple Sequence Repeat Markers In The Dromedary(Camelus Dromedarius) Genome By Next-Generation Sequencing,
2015
TÜBİTAK
Identification Of Simple Sequence Repeat Markers In The Dromedary(Camelus Dromedarius) Genome By Next-Generation Sequencing, Monther Sadder, Hussein Migdadi, Ahmed Al-Haidary, Aly Okab
Turkish Journal of Veterinary & Animal Sciences
The availability of molecular markers in camels is limited. The aim of this study was to develop new simple sequence repeat (SSR) markers. Four breeds of pooled dromedary genome were sequenced at low coverage utilizing Roche and Illumina platforms. A total of 65,746 contigs, covering approximately 52 Mb (2316 contigs > 2 kb), were assembled. The partial genome revealed 613 SSR loci with a minimum number of 5 repeat units. Comparative chromosomal location for 60 camel loci was predicted against bovine genome assembly Baylor Btau_4.6.1/bosTau7. Ten markers (16.7%) returned matches with a >100 score and >80% identity. SSR abundance was 1 …
Association Between The Muc4 G.243a > G Polymorphism And Immune Andproduction Traits In Large White Pigs,
2015
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Association Between The Muc4 G.243a > G Polymorphism And Immune Andproduction Traits In Large White Pigs, Ying Liu, Xue Mei Yin, Ri Wei Xia, Yong Jiu Huo, Guo Qiang Zhu, Sheng Long Wu, Wen Bin Bao
Turkish Journal of Veterinary & Animal Sciences
PCR-RFLP analysis of intron 17 of the MUC4 gene was conducted in Large White pigs and associations with cytokine levels (IFN-?, IL-1ß, IL-4, IL-6, IL-8, IL-10, TGF-ß, and TNF-?), production traits (100-kg-weight day age, back-fat depth, and eye muscle depth), and reproductive performance within different litters (total number born, number born alive, number in live weaning litter) were analyzed with the aim of identifying genetic markers of disease resistance. The results revealed AA, AG, and GG genotypes, with an A243G mutation identified in the GG genotype. Correlation analysis indicated that IL-8 and IL-10 levels and 100-kg-weight day age were significantly …
Report Of Rabies In Feedlot Cattle Introduced Tobaja California From The State Of Guerrero, Mexico,
2015
TÜBİTAK
Report Of Rabies In Feedlot Cattle Introduced Tobaja California From The State Of Guerrero, Mexico, Jose Luis Rodriguez Castillo, Alfonso De La Mora-Valle, Gerardo Enrique Medina-Basulto, Francisco Javier Monge-Navarro, Rosa Maria Bermudez Hurtado, Gilberto Lopez Valencia
Turkish Journal of Veterinary & Animal Sciences
Rabies is a fatal infection of the central nervous system produced by a Lyssavirus of the family Rhabdoviridae. The virus is distributed worldwide and is primarily transmitted by rabid animal bites, with multiple reservoirs depending on the region. The vampire bat Desmodus rotundus is distributed in several states of Mexico and is considered the main vector of rabies in cattle. In the state of Baja California, no cases of paralytic rabies have been reported. In this work, we report the first three cases of rabies in feedlot cattle showing nervous signs, which were later confirmed in a reference laboratory by …
Temperature Range Analysis (T_{Max}) On Dorsal Surface Of Sporting Horses,
2015
TÜBİTAK
Temperature Range Analysis (T_{Max}) On Dorsal Surface Of Sporting Horses, Ewa Jodkowska, Krzysztof Dudek, Maria Soroko
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine temperature differences on the dorsal surface of 32 clinically healthy show jumping horses. Temperature was measured using a Thermovision 550 (FLIR) camera, determining maximum temperature (T_{max}). The horse?s body was divided into the following regions: neck, withers, cranial back, caudal back, loins, and hindquarters. Those regions were subdivided into middle, left, and right parts. Statistical methods used for calculations consisted of Wilcoxon?s signed rank test and Dunn?s post hoc comparison test. Temperatures on the dorsal surface ranged from 19.5 to 31.5 °C, with the lowest indicated in the withers area and the …
Esophageal Obstruction In Water Buffalo (Bubalus Bubalis): A Retrospectivestudy Of 44 Cases (2006?2013),
2015
TÜBİTAK
Esophageal Obstruction In Water Buffalo (Bubalus Bubalis): A Retrospectivestudy Of 44 Cases (2006?2013), Mohamed Marzok, Alaa Moustafa, Sabry El-Khodery, Kerstin Müller
Turkish Journal of Veterinary & Animal Sciences
The purposes of this study were to report the types, causes, and clinical findings of esophageal obstructions in water buffaloes and to verify the role of radiography in diagnosis as well as the evaluation of the proposed treatment management and outcome after treatment. Forty-four buffaloes with esophageal obstruction were used in the study. Esophageal obstruction was significantly more frequent in females than males. Buffaloes at 1-3 years of age were significantly more liable to the disease than those of other ages. Complete obstruction was more frequent than partial obstruction. Intraluminal obstruction was more prevalent than extraluminal. Obstruction at the cervical …
Novel Coproducts From Corn Milling And Their Use In Ruminants? Nutrition,
2015
TÜBİTAK
Novel Coproducts From Corn Milling And Their Use In Ruminants? Nutrition, Catalin Dragomir, Marketta Rinne, David Yanez-Ruiz
Turkish Journal of Veterinary & Animal Sciences
The article reviews published data on two novel coproducts originating from corn milling: high-protein distillers' grain (HPDG) and reduced-fat distillers' grain (RFDG). Based on a literature survey over the last decade, this article focuses on their chemical composition and, consequently, nutritive value and on the effects of their inclusion in ruminants' diets on rumen activity and animal performance. Compared to the classic distillers' grains, the two new coproducts expressed lower variability of their chemical composition, nutritive value, and specific feeding characteristics, allowing more targeted feeding strategies (protein balancing, avoidance of milk fat depression, etc.). Thus, HPDG has significantly higher crude …
The Genetic Diversity Of Both Fecb Gene And Microsatellite Gc101is Associated With Reproduction Selection In Sheep,
2015
TÜBİTAK
The Genetic Diversity Of Both Fecb Gene And Microsatellite Gc101is Associated With Reproduction Selection In Sheep, Zhangyuan Pan, Baoyun Zhang, Wenping Hu, Ran Di, Qiuyue Liu, Xiangyu Wang, Dehua Yin, Pingqing Wang, Mingxing Chu
Turkish Journal of Veterinary & Animal Sciences
The aims of study were to reveal the changes in genetic diversity in reproduction selection of sheep. Genetic polymorphisms of microsatellite GC101 locus and its linkage with the FecB gene were detected in Chinese local sheep (Small Tail Han and Hu sheep) and western sheep (Texel and Dorset) breeds. There was the same FecB mutation (A746G) in both Small Tail Han and Hu sheep as that in Booroola Merino ewes, but not in Texel and Dorset sheep. Seven alleles, from 192 bp to 210 bp, and 20 genotypes were detected at the GC101 locus in 447 individuals totally. The preponderant …
The Relationships Between Brucella Melitensis Predilection Sites, Bacterial Loads In Vivo, And The Agglutinating Antibody Response In Experimentally Infected Sheep, Xiaolei Gao, Yu Kuang, Lintao Ma, Yanli Lu, Qingmin Wu
Turkish Journal of Veterinary & Animal Sciences
F?or exploring Brucella melitensis survival in in vivo predilection sites and the relationship between bacterial loads and the detection of antibody titers in experimentally subcutaneously infected sheep with B.1397645907melitensis 16M, ten rams and ten ewes were used. One ram and one ewe were euthanized at 7, 15, 30, 60, 90, 120, and 180 days post inoculation (dpi). Bacteriological results showed that tissue isolation rates and bacterial loads peaked from 7 dpi to 30 dpi and then cleared until 120 dpi. In in situ hybridization trials the strongest signals for B.1397645907melitensis were detected at 7 dpi and 15 dpi, and then …
Efficacy Of Experimental Inactivated And Live Rhodococcus Equivaccines For Thoroughbred Arabian Mares In Mice,
2015
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Efficacy Of Experimental Inactivated And Live Rhodococcus Equivaccines For Thoroughbred Arabian Mares In Mice, Osman Ergani̇ş, Hasan Hüseyi̇n Hadi̇mli̇, Zafer Sayin, Asli Sakmanoğlu, Yasemi̇n Pinarkara, Özgür Özdemi̇r
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the efficacy of inactive Rhodococcus equi vaccine candidates included that bacterin+aluminum hydroxide (Al(OH)3), bacterin+VapA+(Al(OH)3), bacterin+Montanide IMS 3012 (IMS), bacterin+VapA+IMS, and live vaccine using mice as a model. The efficacy of vaccine was evaluated according to clinical findings, humoral and cellular immunity (levels of INF-g and IL-4), and results of microbiological culture from internal organs in dead or sacrificed mice. Inactive R. Equi vaccines were subcutaneously administered to mice three times at 15-day intervals and live vaccine was intraperitoneally injected once. Fifteen days after the last vaccination, aerosol challenges were carried out with …
False Positive Results Using Pcr Detection Method For African Swinefever Virus In Wild Boars From Northern Romanian Hunting Zones, Vlad Petrovan, Laura Buburuzan, Mihaela Zaulet
Turkish Journal of Veterinary & Animal Sciences
African swine fever virus (ASFV) is an acute virus with a tropism for the pig macrophage and the ability to persist. In the absence of a vaccine, a good understanding of the ecology and epidemiology of the disease is fundamental to implement effective control measures. The recent occurrence and spread of ASFV in East Europe is perceived as a serious risk for the pig industry in the European Union. The aim of this study was to evaluate the classic detection method using polymerase chain reaction (PCR). A total number of 107 wild boar blood, tissues, and organs samples were collected …
