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A Novel Vaccine Candidate Against Rabbit Hemorrhagic Disease Virus 2 (Rhdv2) Confers Protection In Domestic Rabbits, Angela M. Bosco-Lauth, Bethany Cominsky, Stephanie Porter, J. Jeffrey Root, Amber Schueler, Gary Anderson, Sara VanderWal, Andy Benson 2022 Colorado State University

A Novel Vaccine Candidate Against Rabbit Hemorrhagic Disease Virus 2 (Rhdv2) Confers Protection In Domestic Rabbits, Angela M. Bosco-Lauth, Bethany Cominsky, Stephanie Porter, J. Jeffrey Root, Amber Schueler, Gary Anderson, Sara Vanderwal, Andy Benson

USDA Wildlife Services - Staff Publications

OBJECTIVE To evaluate efficacy of a novel vaccine against rabbit hemorrhagic disease virus 2 (RHDV2) in domestic rabbits.

ANIMALS 40 New Zealand White rabbits obtained from a commercial breeder.

PROCEDURES Rabbits were vaccinated and held at the production facility for the duration of the vaccination phase and transferred to Colorado State University for challenge with RHDV2. Rabbits were challenged with oral suspensions containing infectious virus and monitored for clinical disease for up to 10 days. Rabbits that died or were euthanized following infection were necropsied, and livers were evaluated for viral RNA via RT-PCR.

RESULTS None of the vaccinated animals …


Efecto De La Aplicación Intravenosa De Ozono En La Biometría Y Bioquímica Hemática En Caballos Clínicamente Sanos, Alfredo Suárez Chica, José A. Cardona Álvarez, Bernardo José Reyes Bossa 2022 Universidad de Córdoba

Efecto De La Aplicación Intravenosa De Ozono En La Biometría Y Bioquímica Hemática En Caballos Clínicamente Sanos, Alfredo Suárez Chica, José A. Cardona Álvarez, Bernardo José Reyes Bossa

Revista de Medicina Veterinaria

El objetivo del presente estudio es determinar los efectos de la aplicación intravenosa de ozono en la biometría y bioquímica hemática en caballos clínicamente sanos. La investigación fue descriptiva y experimental, de tipo transversal. Asimismo, el tipo de muestreo fue no probabilístico de conveniencia. Se seleccionaron catorce animales, distribuidos en tres grupos: A y B, conformado por seis animales cada uno, y el grupo C, por dos animales. El ozono se aplicó utilizando la autohemoterapia mayor, la sangre fue obtenida de la vena yugular (400 ml), utilizando el kit SANO3® Copyright 2017, Paolo Gobbi Frattini, Srl Italia (todos los derechos …


Validation Of A Method For Pantoprazole And Its Sulfone Metabolite In Goat Plasma Using High Performance Liquid Chromatography, Sherry Cox, Lainey Harvill, Sarah Bullock, Joseph Smith, Joan Bergman 2022 UTK

Validation Of A Method For Pantoprazole And Its Sulfone Metabolite In Goat Plasma Using High Performance Liquid Chromatography, Sherry Cox, Lainey Harvill, Sarah Bullock, Joseph Smith, Joan Bergman

Faculty Publications and Other Works -- Large Animal Clinical Sciences

No abstract provided.


Comparison Of The Humoral Immune Response Following Both Bacterial Challenge And Rnai Of Major Factors On Proliferation Of Bartonella Quintana In The Human Louse, Jake Zina 2022 University of Massachusetts Amherst

Comparison Of The Humoral Immune Response Following Both Bacterial Challenge And Rnai Of Major Factors On Proliferation Of Bartonella Quintana In The Human Louse, Jake Zina

Masters Theses

Human body lice, Pediculus humanus humanus, and head lice, Pediculus humanus capitis, have been hematophagous ectoparasites of humans for thousands of years. Despite being ecotypes, only body lice are known to transmit bacterial diseases to humans, and it appears that lower humoral and cellular immune responses allow body lice to possess a higher vector competence. We previously observed that the transcription level of the defensin 1 gene was up-regulated only in head lice following oral challenge of Bartonella quintana, a causative agent of trench fever, and also that body lice excreted more viable B. quintana in their …


Whole Genome Sequencing Of Moraxella Bovis Strains From North America Reveals Two Genotypes With Different Genetic Determinants, Emily L. Wynn, Matthew M. Hille, John Dustin Loy, Gennie Schuller, Kristen L. Kuhn, Aaron M. Dickey, James L. Bono, Michael L. Clawson 2022 USDA, Agricultural Research Service

Whole Genome Sequencing Of Moraxella Bovis Strains From North America Reveals Two Genotypes With Different Genetic Determinants, Emily L. Wynn, Matthew M. Hille, John Dustin Loy, Gennie Schuller, Kristen L. Kuhn, Aaron M. Dickey, James L. Bono, Michael L. Clawson

Papers in Veterinary and Biomedical Science

Background: Moraxella bovis and Moraxella bovoculi both associate with infectious bovine keratoconjunctivitis (IBK), an economically significant and painful ocular disease that affects cattle worldwide. There are two genotypes of M. bovoculi (genotypes 1 and 2) that differ in their gene content and potential virulence factors, although neither have been experimentally shown to cause IBK. M. bovis is a causative IBK agent, however, not all strains carry a complete assortment of known virulence factors. The goals of this study were to determine the population structure and depth of M. bovis genomic diversity, and to compare core and accessory genes and …


Contrafreeloading In Kea (Nestor Notabilis) In Comparison To Grey Parrots (Psittacus Erithacus), Gabriella E. Smith, Amalia P. M. Bastos, Martin Chodorow, Alex H. Taylor, Irene M. Pepperberg 2022 The Alex Foundation

Contrafreeloading In Kea (Nestor Notabilis) In Comparison To Grey Parrots (Psittacus Erithacus), Gabriella E. Smith, Amalia P. M. Bastos, Martin Chodorow, Alex H. Taylor, Irene M. Pepperberg

Publications and Research

Contrafreeloading—working to access food that could be freely obtained—is rarely exhibited and poorly understood. Based on data from Grey parrots ( Psittacus erithacus ), researchers proposed a correlation between contrafreeloading and play: that contrafreeloading is more likely when subjects view the task as play. We tested that hypothesis by subjecting a relatively more playful parrot species, the kea ( Nestor notabilis ), to the same experimental tasks. Experiment 1 presented eight kea with container pairs holding more- or less-preferred free or enclosed food items, and examined three types of contrafreeloading: calculated (working to access preferred food over less-preferred, freely available …


Activity Of The Lactate Dehydrogenase Inhibitor Oxamic Acid Against The Fermentative Bacterium Streptococcus Mitis/Oralis: Bactericidal Effects And Prevention Of Daptomycin Resistance In Vitro And In An Ex Vivo Model, Razieh Kebriaei, Arnold S. Bayer, Christian K. Lapitan, Michael J. Rybak, Greg A. Somerville, Nagendra N. Mishra 2022 Eugene Applebaum College of Pharmacy and Health Sciences

Activity Of The Lactate Dehydrogenase Inhibitor Oxamic Acid Against The Fermentative Bacterium Streptococcus Mitis/Oralis: Bactericidal Effects And Prevention Of Daptomycin Resistance In Vitro And In An Ex Vivo Model, Razieh Kebriaei, Arnold S. Bayer, Christian K. Lapitan, Michael J. Rybak, Greg A. Somerville, Nagendra N. Mishra

Papers in Veterinary and Biomedical Science

Streptococcus mitis/oralis is a fermentative bacterium that relies on lactate dehydrogenase to balance its redox poise and keep glycolysis active. Metabolomic analysis of an in vitro– derived daptomycin-resistant (DAP-R) S. mitis/oralis strain (351-D10) revealed differences in glucose catabolism relative to its DAP-susceptible (DAP-S) parental strain, 351. Metabolic changes associated with the transition to this DAP-R phenotype suggested that inhibiting glycolysis could alter DAP susceptibility. In addition, the strong reliance of S. mitis/oralis on glycolysis for energy and biosynthetic intermediates suggested that inhibiting glycolysis would adversely affect growth and biomass accumulation. To test these hypotheses, we used the lactate dehydrogenase inhibitor …


A Trichy Situation, Kathryn Purple 2022 Lincoln Memorial University

A Trichy Situation, Kathryn Purple

Cumberland Mountain Naturalist

This issue of the Cumberland Mountain Naturalist discusses Dr Kathryn Purple's research concerning Trichomonosis carried by pigeons.


Gabapentin, A Human Therapeutic Medication And An Environmental Substance Transferring At Trace Levels To Horses: A Case Report., Kimberly Brewer, Jacob Machin, George Maylin, Clara Fenger, Abelardo Morales-Briceño, Thomas Tobin 2022 Wellington, Florida

Gabapentin, A Human Therapeutic Medication And An Environmental Substance Transferring At Trace Levels To Horses: A Case Report., Kimberly Brewer, Jacob Machin, George Maylin, Clara Fenger, Abelardo Morales-Briceño, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Gabapentin, 1-(Aminomethyl)cyclohexaneacetic acid, MW 171.240, is a frequently prescribed high dose human medication that is also used recreationally. Gabapentin is orally absorbed; the dose can be 3,000 mg/day and it is excreted essentially unchanged in urine. Gabapentin is stable in the environment and routinely detected in urban wastewater. Gabapentin randomly transfers from humans to racing horses and is at times detected at pharmacologically ineffective / trace level concentrations in equine plasma and urine. In Ohio racing between January 2019 and July 2020,18 Gabapentin identifications, all less than 2 ng/ml in plasma, were reported. These identifications were ongoing because the horsemen …


Glutathione And Glutaredoxin In Redox Regulation And Cell Signaling Of The Lens, Marjorie F. Lou 2022 University of Nebraska-Lincoln,University of Nebraska Medical Center, University of North Texas Health Science Center

Glutathione And Glutaredoxin In Redox Regulation And Cell Signaling Of The Lens, Marjorie F. Lou

Papers in Veterinary and Biomedical Science

The ocular lens has a very high content of the antioxidant glutathione (GSH) and the enzymes that can recycle its oxidized form, glutathione disulfide (GSSG), for further use. It can be synthesized in the lens and, in part, transported from the neighboring anterior aqueous humor and posterior vitreous body. GSH is known to protect the thiols of the structural lens crystallin proteins from oxidation by reactive oxygen species (ROS) so the lens can maintain its transparency for proper visual function. Age-related lens opacity or senile cataract is the major visual impairment in the general population, and its cause is closely …


The Ethical Perceptions Of Dog Tail Docking Amongst Owners And Veterinarians In South Carolina, Hannah Ellen Steinberg 2022 University of South Carolina - Columbia

The Ethical Perceptions Of Dog Tail Docking Amongst Owners And Veterinarians In South Carolina, Hannah Ellen Steinberg

Senior Theses

This thesis aimed to discover, in depth, what tail docking of dogs is, why it is currently in practice, what the controversial opinions surrounding it are, how the opinions differ when taking into account the professionals of the field versus owners and breeders, and how it can be addressed in the future to treat our animals as humanely as possible.

Based on the surveying of South Carolinian owners, breeders, and veterinarians, it was discovered that dog tail-docking procedures are overwhelmingly used for cosmetic or aesthetic purposes, with the aim being to match breed standards. There was a disagreement amongst those …


Seasonal Habitat Selection By American White Pelicans, Frederick L. Cunningham, Guiming Wang, D. Tommy King 2022 USDA National Wildlife Research Center

Seasonal Habitat Selection By American White Pelicans, Frederick L. Cunningham, Guiming Wang, D. Tommy King

USDA Wildlife Services - Staff Publications

Resource utilization strategies of avian migrants are a major concern for conservation and management. Understanding seasonal habitat selection by migratory birds helps us explain the ongoing continental declines of migratory bird populations. Our objective was to compare the secondorder and third-order habitat selection by the American White Pelican (Pelecanus erythrorhynchos; hereafter pelican) between the breeding and non-breeding grounds. We tested the Lack hypothesis that habitat selection by migratory birds is stronger on the breeding grounds than on the nonbreeding grounds. We used random-effect Dirichlet-multinomial models to estimate the second-order habitat selection between the seasons with the GPS locations …


Effects Of Early‑Life Experience On Innovation And Problem‑Solving In Captive Coyotes, Andrew C. Garcia, Mitchell A. Parsons, Julie K. Young 2022 USDA National Wildlife Research Center

Effects Of Early‑Life Experience On Innovation And Problem‑Solving In Captive Coyotes, Andrew C. Garcia, Mitchell A. Parsons, Julie K. Young

USDA Wildlife Services - Staff Publications

Early-life experience often shapes behaviors like innovation and exploration. These behaviors are important to animals encountering novel food resources in diverse habitats, such as mesocarnivores in urban areas. To understand if early-life experiences impact later-life behavior, we examined how coyotes (Canis latrans) responded to a multi-access puzzle box at two life stages: pup (~ 7 weeks) and dispersal (~ 10 months). We first exposed pups, still living with their parents and littermates, to a baited puzzle box. At dispersal age, we again tested both these pups and an age-matched control group that was not exposed to the puzzle …


Recent Emergence Of Bovine Coronavirus Variants With Mutations In The Hemagglutinin-Esterase Receptor Binding Domain In U.S. Cattle, Aspen M. Workman, Tara G. McDaneld, Gregory P. Harhay, Subha Das, John Dustin Loy, Benjamin M. Hause 2022 USDA, Agricultural Research Service

Recent Emergence Of Bovine Coronavirus Variants With Mutations In The Hemagglutinin-Esterase Receptor Binding Domain In U.S. Cattle, Aspen M. Workman, Tara G. Mcdaneld, Gregory P. Harhay, Subha Das, John Dustin Loy, Benjamin M. Hause

Papers in Veterinary and Biomedical Science

Bovine coronavirus (BCoV) has spilled over to many species, including humans, where the host range variant coronavirus OC43 is endemic. The balance of the opposing activities of the surface spike (S) and hemagglutinin-esterase (HE) glycoproteins controls BCoV avidity, which is critical for interspecies transmission and host adaptation. Here, 78 genomes were sequenced directly from clinical samples collected between 2013 and 2022 from cattle in 12 states, primarily in the Midwestern U.S. Relatively little genetic diversity was observed, with genomes having >98% nucleotide identity. Eleven isolates collected between 2020 and 2022 from four states (Nebraska, Colorado, California, and Wisconsin) contained a …


Making The Case: Adding A Social Work Perspective To A Case Study In A Veterinary Practice, Paula Gerstenblatt, Diane Rhodes, Dorothea Ivey 2022 University of Southern Maine

Making The Case: Adding A Social Work Perspective To A Case Study In A Veterinary Practice, Paula Gerstenblatt, Diane Rhodes, Dorothea Ivey

People and Animals: The International Journal of Research and Practice

This article describes a case study exploration of a veterinary practice from the perspective of social work. Recognizing the stressful work of veterinary providers, including compassion fatigue and high suicide rates, the current study identified both a need for and the potential role of social workers in veterinary practice. Professionals were interviewed in an emergency/specialty practice. Using the interview data, the authors built a demonstrative case that underlines the vulnerability of veterinary professionals and the potential of social work to improve the quality of their work experiences and longevity.


Understanding The Causative Agent, Pathophysiology And Management Of The Kentucky Mare Reproductive Loss Syndrome And Related Syndromes, Thomas Tobin, Gabriel Volpato, Navid Yousefi-Mashouf, Kimberly Brewer 2022 University of Kentucky

Understanding The Causative Agent, Pathophysiology And Management Of The Kentucky Mare Reproductive Loss Syndrome And Related Syndromes, Thomas Tobin, Gabriel Volpato, Navid Yousefi-Mashouf, Kimberly Brewer

Maxwell H. Gluck Equine Research Center Faculty Presentations

No abstract provided.


Pharmacokinetics Of Butorphanol Following Intravenous And Intramuscular Administration In Donkeys: A Preliminary Study, Lisa S. Ebner, Odette O, Bradley Simon, Ignacio Lizarraga, Joseph Smith, Sherry Cox 2022 Lincoln Memorial University

Pharmacokinetics Of Butorphanol Following Intravenous And Intramuscular Administration In Donkeys: A Preliminary Study, Lisa S. Ebner, Odette O, Bradley Simon, Ignacio Lizarraga, Joseph Smith, Sherry Cox

Faculty Publications and Other Works -- Large Animal Clinical Sciences

The pharmacokinetics of butorphanol after intravenous (IVB) and intramuscular (IMB) administration in donkeys were determined in this preliminary study. Healthy male gelded donkeys (n = 5), aged 6–12 years old, were administered 0.1 mg/kg butorphanol IV or IM in a randomized, crossover design. Blood samples were obtained at predetermined intervals for 24 h (IVB) and 48 h (IMB) after administration. Plasma butorphanol concentrations were determined by high performance liquid chromatography and pharmacokinetic parameters were calculated. Following IVB administration, mean (± SE) apparent volume of distribution, elimination half-life, total body clearance, and area under the plasma concentration time curve from …


Semen Production Capacity Among Boar Breeds And Effect Of Organic Antioxidant Supplementation On Sperm Characteristics During The Hot Season In Thailand, CongBang Ngo, Suriya Sooksong, Nutthee Am-in, Padet Tummaruk 2022 Chulalongkorn University

Semen Production Capacity Among Boar Breeds And Effect Of Organic Antioxidant Supplementation On Sperm Characteristics During The Hot Season In Thailand, Congbang Ngo, Suriya Sooksong, Nutthee Am-In, Padet Tummaruk

The Thai Journal of Veterinary Medicine

The present study was carried out to investigate semen production capacity among boar breeds and measure the efficiency of a new organic antioxidant supplementation on semen production and qualities during the hot season in Thailand. A total of 711 ejaculates from 87 boars (i.e., 26 Duroc, 15 Landrace, 30 Yorkshire and 16 crossbred Landrace × Yorkshire) were included in the study. Semen samples were allocated into two treatments – without organic antioxidant (CONTROL; n = 397) and with organic antioxidant supplementation (TREATMENT; n = 314). The experiment was carried out for three months during the hot season in May (n …


Application Of A Single-Tank Sequencing Batch Reactor For Long-Term Zebrafish Care, Aranya Ponpornpisit, Malinee Jongjaroenjai, Naowarat Suthamnatpong, Surachet Burut-Archanai 2022 Chulalongkorn University

Application Of A Single-Tank Sequencing Batch Reactor For Long-Term Zebrafish Care, Aranya Ponpornpisit, Malinee Jongjaroenjai, Naowarat Suthamnatpong, Surachet Burut-Archanai

The Thai Journal of Veterinary Medicine

Sequencing batch reactors are efficient wastewater treatment systems, however, they are not adapted to treat aquaria. We aimed to compare the efficacies of a modified single-tank sequencing batch reactor (SSBR) and a regular overflow filtration system. Zebrafish were maintained in an acrylic aquarium connected to an SSBR or in a tank connected to a regular overflow filtration system for 27 weeks. Water change or aeration using air stone was not performed. The water quality in both groups was within the normal range until 26 weeks, after which the nitrite and nitrate levels increased. The average temperature was, 29.46 ± 0.71°C …


Microbial Diversity And Microbiological Quality Of Edible-Birdnest (Ebn) During Processing And Storage, Shazreena Zuber, Zunita Zakaria, Jalila Abu, Saleha Abdul Aziz 2022 Chulalongkorn University

Microbial Diversity And Microbiological Quality Of Edible-Birdnest (Ebn) During Processing And Storage, Shazreena Zuber, Zunita Zakaria, Jalila Abu, Saleha Abdul Aziz

The Thai Journal of Veterinary Medicine

The microbial quality of 45 EBN consisting of raw unclean and raw clean Edible-birdnest (EBN) from two different premises were analyzed for 8 weeks. Using 3M™ Petrifilm, Total Aerobic Count (TAC) of raw clean EBN and Total Coliform Count (TCC) for raw unclean, 3 days, 2 weeks and 4 weeks EBN in both premises exceeded the limit stated in by the Malaysian Standard (MS 2334:2011; SIRIM/DVS 3:2015). A total of 264 bacteria and 208 fungi species were isolated. Bacteria isolated was identified using bioMérieux’s API® kit and a series of biochemical tests. Fungi were identified using techniques described by the …


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