Pharmacy and Pharmaceutical Sciences Commons^™

Library Impact Statement For Bps 555 Protein Molecular Modeling, Michael Vocino

Library Impact Statements

Library Impact Statement for BPS 555 Protein Molecular Modeling. No new resources were required to support this course.

Assessing The Impact Of Nutritional And Exercise Recommendations On Health Fair Participants: Follow-Up Program: Partnering Up For A Healthier You, Amy Mcmanness

Undergraduate Honors Thesis Collection

The primary objective of our study was to estimate the impact pharmacy students can have through the provision of educational fitness and nutrition materials and body composition assessments to increase knowledge in the general population at various health fairs throughout Indianapolis. The secondary objective was to assess the impact of follow-up phone calls on patient success towards personal health goals. It also provided an accountability and information resource to patients enrolled in the follow-up program as well as gauged the overall acceptance of pharmacists in this community role.

Engineered Nanomaterials: Exposures, Hazards, And Risk Prevention, Robert A. Yokel, Robert C. Macphail

Pharmaceutical Sciences Faculty Publications

Nanotechnology presents the possibility of revolutionizing many aspects of our lives. People in many settings (academic, small and large industrial, and the general public in industrialized nations) are either developing or using engineered nanomaterials (ENMs) or ENM-containing products. However, our understanding of the occupational, health and safety aspects of ENMs is still in its formative stage. A survey of the literature indicates the available information is incomplete, many of the early findings have not been independently verified, and some may have been over-interpreted. This review describes ENMs briefly, their application, the ENM workforce, the major routes of human exposure, some …

Role Of The At2 Receptor In Modulating The Angiotensin Ii Contractile Response Of The Uterine Artery At Mid-Gestation, V. M. Pulgar, H. Yamashiro, J. C. Rose, L. G. Moore

Pharmaceutical Sciences

Introduction: During human pregnancy, circulating concentrations of components of the renin—angiotensin system increase, but pressor refractoriness to angiotensin II (Ang-II) is observed. Given the importance of the Ang-II pressor response in deciding susceptibility to preeclampsia and of the Ang-II system for controlling uterine vasoreactivity, we sought to address the effects of pregnancy on the reactivity of the isolated uterine artery (UA) in mice.

Materials and methods : Blood pressure was measured throughout pregnancy in awake C57BL/6J mice. UA segments were isolated from three groups of animals (non-pregnant, mid [day 12—13] and late [day 18—19] gestation) and studied by wire …

Role Of The At2 Receptor In Modulating The Angiotensin Ii Contractile Response Of The Uterine Artery At Mid-Gestation, V. M. Pulgar, H. Yamashiro, J. C. Rose, L. G. Moore

Pharmaceutical Sciences

No abstract provided.

Differential Subcellular Distribution Of Rat Brain Dopamine Receptors And Subtype-Specific Redistribution Induced By Cocaine., Pamela J Voulalas, John Schetz, Ashiwel S Undieh

College of Pharmacy Faculty Papers

We investigated the subcellular distribution of dopamine D(1), D(2) and D(5) receptor subtypes in rat frontal cortex, and examined whether psychostimulant-induced elevation of synaptic dopamine could alter the receptor distribution. Differential detergent solubilization and density gradient centrifugation were used to separate various subcellular fractions, followed by semi-quantitative determination of the relative abundance of specific receptor proteins in each fraction. D(1) receptors were predominantly localized to detergent-resistant membranes, and a portion of these receptors also floated on sucrose gradients. These properties are characteristic of proteins found in lipid rafts and caveolae. D(2) receptors exhibited variable distribution between cytoplasmic, detergent-soluble and detergent-resistant …

Potential Role Of Tigecycline In The Treatment Of Community-Acquired Bacterial Pneumonia, M. L. Townsend, M. W. Pound, R. H. Drew

Pharmacy Practice

Tigecycline is a member of the glycylcycline class of antimicrobials, which is structurally similar to the tetracycline class. It demonstrates potent in vitro activity against causative pathogens that are most frequently isolated in patients with community-acquired bacterial pneumonia (CABP), including (but not limited to) Streptococcus pneumoniae (both penicillin-sensitive and -resistant strains), Haemophilus influenzae and Moraxella catarrhalis (including β-lactamase-producing strains), Klebsiella pneumoniae, and ‘atypical organisms’ (namely Chlamydophila pneumoniae, Mycoplasma pneumoniae, and Legionella pneumophila). Comparative randomized clinical trials to date performed in hospitalized patients receiving tigecycline 100 mg intravenous (IV) × 1 and then 50 mg IV twice daily thereafter have demonstrated …

Surface-Enhanced Raman Imaging Of Intracellular Bioreduction Of Chromate, S. P. Ravindranath, K. L. Henne, D. K. Thompson, J. Irudayaraj

Pharmaceutical Sciences

No abstract provided.

High Activity Mutants Of Butyrylcholinesterase For Cocaine Hydrolysis, Chang-Guo Zhan, Fang Zheng, Wenchao Yang

Pharmaceutical Sciences Faculty Patents

Butyrylcholinesterase (BChE) polypeptide variants of the presently-disclosed subject matter have enhanced catalytic efficiency for (−)-cocaine, as compared to wild-type BChE. Pharmaceutical compositions of the presently-disclosed subject matter include a BChE polypeptide variant having an enhanced catalytic efficiency for (−)-cocaine. A method of the presently-disclosed subject matter for treating a cocaine-induced condition includes administering to an individual an effective amount of a BChE polypeptide variant, as disclosed herein, to lower blood cocaine concentration.

Effects Of Androgen Receptor And Androgen On Gene Expression In Prostate Stromal Fibroblasts And Paracrine Signaling To Prostate Cancer Cells, Matthew J. Tanner, R. Charles Welliver, Mengqian Chen, Michael Shtutman, Alejandro Godoy, Gary Smith, Badar M. Mian, Ralph Buttyan

Faculty Publications

The androgen receptor (AR) is expressed in a subset of prostate stromal cells and functional stromal cell AR is required for normal prostate developmental and influences the growth of prostate tumors. Although we are broadly aware of the specifics of the genomic actions of AR in prostate cancer cells, relatively little is known regarding the gene targets of functional AR in prostate stromal cells. Here, we describe a novel human prostate stromal cell model that enabled us to study the effects of AR on gene expression in these cells. The model involves a genetically manipulated variant of immortalized human WPMY-1 …

The Role Of Histone H4 Biotinylation In The Structure Of Nucleosomes., Nina A. Filenko, Carol Kolar, John T. West, S. Abbie Smith, Yousef I. Hassan, Gloria E.O. Borgstahl, Janos Zempleni, Yuri L. Lyubchenko

Journal Articles: Pharmaceutical Sciences

BACKGROUND: Post-translational modifications of histones play important roles in regulating nucleosome structure and gene transcription. It has been shown that biotinylation of histone H4 at lysine-12 in histone H4 (K12Bio-H4) is associated with repression of a number of genes. We hypothesized that biotinylation modifies the physical structure of nucleosomes, and that biotin-induced conformational changes contribute to gene silencing associated with histone biotinylation.

METHODOLOGY/PRINCIPAL FINDINGS: To test this hypothesis we used atomic force microscopy to directly analyze structures of nucleosomes formed with biotin-modified and non-modified H4. The analysis of the AFM images revealed a 13% increase in the length of DNA …

Unifying The Mathematical Modeling Of In Vivo And In Vitro Microdialysis, Peter M. Bungay, Rachita K. Sumbria, Ulrich Bickel

Pharmacy Faculty Articles and Research

A unifying approach is presented for developing mathematical models of microdialysis that are applicable to both in vitro and in vivo situations. Previous models for cylindrical probes have been limited by accommodating analyte diffusion through the surrounding medium in the radial direction only, i.e., perpendicular to the probe axis, or by incomplete incorporation of diffusion in the axial direction. Both radial and axial diffusion are included in the present work by employing two-dimensional finite element analysis. As in previous models, the nondimensional clearance modulus (Θ) represents the degree to which analyte clearance from the external medium influences diffusion through the …

Bound Water At Protein-Protein Interfaces: Partners, Roles And Hydrophobic Bubbles As A Conserved Motif, Mostafa H. Ahmed, Francesca Spyrakis, Pietro Cozzini, Parijat K. Tripathi, Andrea Mozzarelli, J. Neel Scarsdale, Martin A. Safo, Glen E. Kellogg

Medicinal Chemistry Publications

Background

There is a great interest in understanding and exploiting protein-protein associations as new routes for treating human disease. However, these associations are difficult to structurally characterize or model although the number of X-ray structures for protein-protein complexes is expanding. One feature of these complexes that has received little attention is the role of water molecules in the interfacial region.

Methodology

A data set of 4741 water molecules abstracted from 179 high-resolution (≤ 2.30 Å) X-ray crystal structures of protein-protein complexes was analyzed with a suite of modeling tools based on the HINT forcefield and hydrogen-bonding geometry. A metric termed …

Racial Influence On Abcb1 Gene Expression In Peripheral Blood Mononuclear Cells In Stable Renal Transplant Recipients, Daniel A. Brazeau, Kathleen Tornatore, Aijaz Gundroo, Rocco Venuto

Pharmaceutical Sciences Faculty Posters

Background: Immunosuppressive therapy (IT) such as tacrolimus are influenced by pglycoprotein (P-gp) which modulates cellular efflux of this drug. P-gp is present on peripheral mononuclear cells (PBMC) and is encoded by the ABCB1 gene. No data are available regarding the impact of race on ABCB1 gene expression in PBMCs post-transplant over IT dosing interval. Methods: An observational study was completed in 20 African American (AA) and 11 Caucasian (C) stable renal transplant recipients (RTR) (ages 30-74 yrs) receiving tacrolimus (trough: 5 - 10 ng /ml), and enteric coated mycophenolate sodium. At time 0 (prior to IT) & 4, …

From Good Intentions To Proven Interventions: Effectiveness Of Actions To Reduce The Health Impacts Of Air Pollution, Luisa V. Giles, Prabjit Barn, Nino Kuenzli, Isabelle Romieu, Murray A. Mittleman, Stephan Van Eeden, Ryan Allen, Chris Carlsten, Dave Stieb, Curtis W. Noonan, Audrey Smargiassi, Joel D. Kaufman, Shakoor Hajat, Tom Kosatsky, Michael Brauer

Biomedical and Pharmaceutical Sciences Faculty Publications

BACKGROUND: Associations between air pollution and a multitude of health effects are now well established. Given ubiquitous exposure to some level of air pollution, the attributable health burden can be high, particularly for susceptible populations. OBJECTIVES: An international multidisciplinary workshop was convened to discuss evidence of the effectiveness of actions to reduce health impacts of air pollution at both the community and individual level. The overall aim was to summarize current knowledge regarding air pollution exposure and health impacts leading to public health recommendations. DISCUSSION: During the workshop, experts reviewed the biological mechanisms of action of air pollution in the …

The Central Cavity In Trimeric Glutamate Transporters Restricts Ligand Diffusion, Gregory Patrick Leary, David Charles Holley, Emily F. Stone, Brent Russell Lyda, Leonid Kalachev, Michael Kavanaugh

Biomedical and Pharmaceutical Sciences Faculty Publications

A prominent aqueous cavity is formed by the junction of three identical subunits in the excitatory amino acid transporter (EAAT) family. To investigate the effect of this structure on the interaction of ligands with the transporter, we recorded currents in voltage-clamped Xenopus oocytes expressing EAATs and used concentration jumps to measure binding and unbinding rates of a high-affinity aspartate analog that competitively blocks transport (β-2-fluorenyl-aspartylamide; 2-FAA). The binding rates of the blocker were approximately one order of magnitude slower than l-Glu and were not significantly different for EAAT1, EAAT2, or EAAT3, but 2-FAA exhibited higher affinity for the neuronal transporter …

Applying An Empirical Hydropathic Forcefield In Refinement May Improve Low-Resolution Protein X-Ray Crystal Structures, Vishal N. Koparde, J. Neel Scarsdale, Glen E. Kellogg

Medicinal Chemistry Publications

Background

The quality of X-ray crystallographic models for biomacromolecules refined from data obtained at high-resolution is assured by the data itself. However, at low-resolution, >3.0 Å, additional information is supplied by a forcefield coupled with an associated refinement protocol. These resulting structures are often of lower quality and thus unsuitable for downstream activities like structure-based drug discovery.

Methodology

An X-ray crystallography refinement protocol that enhances standard methodology by incorporating energy terms from the HINT (Hydropathic INTeractions) empirical forcefield is described. This protocol was tested by refining synthetic low-resolution structural data derived from 25 diverse high-resolution structures, and referencing the resulting …

No Complaining..., James R. Krebs, Gayle A. Brazeau

Pharmacy Practice Faculty Publications

It is said that hunger is a great motivator. If only the hunger for knowledge were as great as the hunger for physical sustenance. This statement could be lodged as a complaint, but that would defeat the purpose of this essay. How is it possible to turn the toil of our learning into the enlightenment for self-improvement?

An Epichromatin Epitope: Persistence In The Cell Cycle And Conservation In Evolution, Ada L. Olins, Markus Langhans, Marc Monestier, Andreas Schlotterer, David G. Robinson, Corrado Viotti, Hanswalter Zentgraf, Monika Zwerger, Donald E. Olins

Pharmaceutical Sciences Faculty Publications

Interphase nuclear architecture is disrupted and rapidly reformed with each cell division cycle. Successive cell generations exhibit a “memory” of this nuclear architecture, as well as for gene expression. Furthermore, many features of nuclear and mitotic chromosome structure are recognizably species and tissue specific. We wish to know what properties of the underlying chromatin structure may determine these conserved features of nuclear architecture. Employing a particular mouse autoimmune anti-nucleosome monoclonal antibody (PL2-6), combined with deconvolution immunofluorescence microscopy, we present evidence for a unique epitope (involving a ternary complex of histones H2A and H2B and DNA) which is localized only at …

Preliminary Report On Patent Literature, Search Methodology And Patent Status Of Medicines On The Who Eml 2009, Jon R. Cavicchi, Stanley P. Kowalski

Law Faculty Scholarship

Over the past several decades the World Health Organization (WHO) has produced the Essential Medicines List (EML) to assist countries in deciding what medicines should be essential and available in National Essential Medicine Lists.1 WHO, through the work of regional offices, supports nations using the EML to ensure the quality, availability, and affordability of pharmaceuticals required to promote and advance public health in nations across the globe. However in some cases, access to EML pharmaceuticals might be complicated by existing patents, i.e., where issued, patent rights might pose obstacles to access and inclusion in national EMLs. Indeed, in developed and …