Pharmacy and Pharmaceutical Sciences Commons^™

Bound Water At Protein-Protein Interfaces: Partners, Roles And Hydrophobic Bubbles As A Conserved Motif, Mostafa H. Ahmed, Francesca Spyrakis, Pietro Cozzini, Parijat K. Tripathi, Andrea Mozzarelli, J. Neel Scarsdale, Martin A. Safo, Glen E. Kellogg

Medicinal Chemistry Publications

Background

There is a great interest in understanding and exploiting protein-protein associations as new routes for treating human disease. However, these associations are difficult to structurally characterize or model although the number of X-ray structures for protein-protein complexes is expanding. One feature of these complexes that has received little attention is the role of water molecules in the interfacial region.

Methodology

A data set of 4741 water molecules abstracted from 179 high-resolution (≤ 2.30 Å) X-ray crystal structures of protein-protein complexes was analyzed with a suite of modeling tools based on the HINT forcefield and hydrogen-bonding geometry. A metric termed …

Racial Influence On Abcb1 Gene Expression In Peripheral Blood Mononuclear Cells In Stable Renal Transplant Recipients, Daniel A. Brazeau, Kathleen Tornatore, Aijaz Gundroo, Rocco Venuto

Pharmaceutical Sciences Faculty Posters

Background: Immunosuppressive therapy (IT) such as tacrolimus are influenced by pglycoprotein (P-gp) which modulates cellular efflux of this drug. P-gp is present on peripheral mononuclear cells (PBMC) and is encoded by the ABCB1 gene. No data are available regarding the impact of race on ABCB1 gene expression in PBMCs post-transplant over IT dosing interval. Methods: An observational study was completed in 20 African American (AA) and 11 Caucasian (C) stable renal transplant recipients (RTR) (ages 30-74 yrs) receiving tacrolimus (trough: 5 - 10 ng /ml), and enteric coated mycophenolate sodium. At time 0 (prior to IT) & 4, …

From Good Intentions To Proven Interventions: Effectiveness Of Actions To Reduce The Health Impacts Of Air Pollution, Luisa V. Giles, Prabjit Barn, Nino Kuenzli, Isabelle Romieu, Murray A. Mittleman, Stephan Van Eeden, Ryan Allen, Chris Carlsten, Dave Stieb, Curtis W. Noonan, Audrey Smargiassi, Joel D. Kaufman, Shakoor Hajat, Tom Kosatsky, Michael Brauer

Biomedical and Pharmaceutical Sciences Faculty Publications

BACKGROUND: Associations between air pollution and a multitude of health effects are now well established. Given ubiquitous exposure to some level of air pollution, the attributable health burden can be high, particularly for susceptible populations. OBJECTIVES: An international multidisciplinary workshop was convened to discuss evidence of the effectiveness of actions to reduce health impacts of air pollution at both the community and individual level. The overall aim was to summarize current knowledge regarding air pollution exposure and health impacts leading to public health recommendations. DISCUSSION: During the workshop, experts reviewed the biological mechanisms of action of air pollution in the …

The Central Cavity In Trimeric Glutamate Transporters Restricts Ligand Diffusion, Gregory Patrick Leary, David Charles Holley, Emily F. Stone, Brent Russell Lyda, Leonid Kalachev, Michael Kavanaugh

Biomedical and Pharmaceutical Sciences Faculty Publications

A prominent aqueous cavity is formed by the junction of three identical subunits in the excitatory amino acid transporter (EAAT) family. To investigate the effect of this structure on the interaction of ligands with the transporter, we recorded currents in voltage-clamped Xenopus oocytes expressing EAATs and used concentration jumps to measure binding and unbinding rates of a high-affinity aspartate analog that competitively blocks transport (β-2-fluorenyl-aspartylamide; 2-FAA). The binding rates of the blocker were approximately one order of magnitude slower than l-Glu and were not significantly different for EAAT1, EAAT2, or EAAT3, but 2-FAA exhibited higher affinity for the neuronal transporter …

Applying An Empirical Hydropathic Forcefield In Refinement May Improve Low-Resolution Protein X-Ray Crystal Structures, Vishal N. Koparde, J. Neel Scarsdale, Glen E. Kellogg

Medicinal Chemistry Publications

Background

The quality of X-ray crystallographic models for biomacromolecules refined from data obtained at high-resolution is assured by the data itself. However, at low-resolution, >3.0 Å, additional information is supplied by a forcefield coupled with an associated refinement protocol. These resulting structures are often of lower quality and thus unsuitable for downstream activities like structure-based drug discovery.

Methodology

An X-ray crystallography refinement protocol that enhances standard methodology by incorporating energy terms from the HINT (Hydropathic INTeractions) empirical forcefield is described. This protocol was tested by refining synthetic low-resolution structural data derived from 25 diverse high-resolution structures, and referencing the resulting …

No Complaining..., James R. Krebs, Gayle A. Brazeau

Pharmacy Practice Faculty Publications

It is said that hunger is a great motivator. If only the hunger for knowledge were as great as the hunger for physical sustenance. This statement could be lodged as a complaint, but that would defeat the purpose of this essay. How is it possible to turn the toil of our learning into the enlightenment for self-improvement?

An Epichromatin Epitope: Persistence In The Cell Cycle And Conservation In Evolution, Ada L. Olins, Markus Langhans, Marc Monestier, Andreas Schlotterer, David G. Robinson, Corrado Viotti, Hanswalter Zentgraf, Monika Zwerger, Donald E. Olins

Pharmaceutical Sciences Faculty Publications

Interphase nuclear architecture is disrupted and rapidly reformed with each cell division cycle. Successive cell generations exhibit a “memory” of this nuclear architecture, as well as for gene expression. Furthermore, many features of nuclear and mitotic chromosome structure are recognizably species and tissue specific. We wish to know what properties of the underlying chromatin structure may determine these conserved features of nuclear architecture. Employing a particular mouse autoimmune anti-nucleosome monoclonal antibody (PL2-6), combined with deconvolution immunofluorescence microscopy, we present evidence for a unique epitope (involving a ternary complex of histones H2A and H2B and DNA) which is localized only at …

Preliminary Report On Patent Literature, Search Methodology And Patent Status Of Medicines On The Who Eml 2009, Jon R. Cavicchi, Stanley P. Kowalski

Law Faculty Scholarship

Over the past several decades the World Health Organization (WHO) has produced the Essential Medicines List (EML) to assist countries in deciding what medicines should be essential and available in National Essential Medicine Lists.1 WHO, through the work of regional offices, supports nations using the EML to ensure the quality, availability, and affordability of pharmaceuticals required to promote and advance public health in nations across the globe. However in some cases, access to EML pharmaceuticals might be complicated by existing patents, i.e., where issued, patent rights might pose obstacles to access and inclusion in national EMLs. Indeed, in developed and …

Influence Of Continuous Venovenous Hemofiltration And Continuous Venovenous Hemodiafiltration On The Disposition Of Doripenem, Iolanda Cirillo, Nicole Vaccaro, Dainius Balis, Rebecca Redman, Gary R. Matzke

Pharmacotherapy and Outcomes Science Publications

The pharmacokinetics, safety, and tolerability of a single 1-hour, 500-mg intravenous infusion of doripenem were assessed in dialysis-dependent subjects with stage 5 chronic kidney disease undergoing continuous renal replacement therapy (CRRT) via 12-hour continuous venovenous hemofiltration (CVVH) (n = 6) or continuous venovenous hemodiafiltration (CVVHDF) (n = 5). Healthy volunteers were also assessed (n = 12). Concentrations of doripenem and the primary metabolite doripenem-M-1 were measured in plasma and ultrafiltrate or ultrafiltrate/dialysate by a validated liquid chromatography-tandem mass spectrometry method. In dialysis-dependent subjects, levels of systemic exposure to doripenem and doripenem-M-1 were approximately 3- and 5-fold greater, respectively, than those …

2011 Apothecary, Southwestern Oklahoma State University

Apothecary

This publication is dedicated to the Southwestern College of Pharmacy faculty, staff, alumni, and students. We congratulate this year's graduating seniors and wish you success in your professional careers.

The 2011 issue of the Apothecary was made possible by the generous financial contributions of many stores, businesses, as well as the Southwestern College of Pharmacy alumni. We are very grateful to all of you for your continued support.

Anthocyanins, Phenolics, And Antioxidant Capacity Of Vaccinium L. In Texas, Usa, Wei Yuan, Lijing Zhou, Guangrui Deng, Ping Wang, David Creech, Shiyou Li

Faculty Publications

Berries of Vaccinium spp. have long been an important source of food and pharmaceutical ingredients and are considered to have high antioxidant potential. Growing blueberries in Texas, United States is a substantial industry, but there is no report on their antioxidant capacity, total phenolics, and anthocyanins. This study evaluates antioxidant capacity and contents of total phenolics and anthocayanins in both fruits and leaves of 19 genotypes including five commercial cultivars and 11 selections of rabbiteye blueberry (V. ashei) and southern highbush (V. corymbosum hybrids), and three native species (V. darrowii, V. arboreum, and V. fuscatum) grown in Nacogdoches, Texas. Significant …

Preparation And Characterization Of A Standardized Anticonvulsant Ginseng Rb Extract From Panax Quinquefolius L., Xiao-Yuan Lian, Zhizhen Zhang

Faculty Publications

A ginseng Rb extract (GRbE) containing three major ingredients of ginsenoside Rb1 (G-Rb1), ginsenoside Rb3 (G-Rb3) and ginsenoside Rd (G-Rd) has been shown to have anticonvulsant and neuroprotective activity. As such, sufficient characterization and standardization of this active GRbE are demanded to facilitate an ongoing preclinical investigation on its potential for the treatment of epilepsy. In the investigation, the methods for generating the active GRbE and isolating G-Rb1, G-Rb3, and G-Rd in large scale from Panax quinquefolius are described. The chemical profile of GRbE was characterized by identifying the structure of the individual ingredients using NMR, and the concentration of …

Morinda Citrifolia L. (Noni) Improves The Quality Of Life In Adults With Osteoarthritis, Mian-Ying Wang, M. Nawal Lutfiyya, Vicki Weidenbacher-Hoper, Lin Peng, Martin S. Lipsky, Gary Anderson

Community Health Faculty Publications and Presentations

Background: Morinda citrifolia Linn (noni), as a “pain killer”, has been used as a traditional medicine by Polynesians for over 2000 years. It was reported to have a broad range of therapeutic effects including analgesic and anti-inflammation. The in-vitro and in vivoanti-inflammatory and analgesic properties of noni juice (NJ) suggest that NJ may be a useful adjunctive treatment for osteoarthritis (OA). In this pilot study we explored whether NJ improves the symptoms and Quality of Life (QoL) for adults with OA. We also sought to evaluate the tolerability and safety of NJ for patients with OA in …

Efficacy Of Limited Cefuroxime Prophylaxis In Pediatric Patients After Cardiovascular Surgery, Chad A. Knoderer, Elaine G. Cox, Michelle D. Berg, Andrea H. Webster, Mark W. Turrentine

Scholarship and Professional Work – COPHS

Purpose The efficacy of limited cefuroxime prophylaxis in pediatric patients after cardiovascular surgery was evaluated.

Methods All patients age 18 years or younger who underwent cardiovascular surgery and received postoperative care from the cardiovascular surgery team between February and July 2006 (preintervention group) and between August 2006 and January 2007 (postintervention group) were eligible for study inclusion. Patients were excluded if they did not receive cefuroxime as postoperative prophylaxis, had a preexisting infection, underwent cardiac transplantation or extracorporeal membrane oxygenation, or underwent delayed sternal closure. The preintervention group received prolonged cefuroxime prophylaxis, and the postintervention group received 24 hours of …

Back Talk: The Medicine Cabinet, Dane L. Shiltz

Scholarship and Professional Work – COPHS

Presenation to pharmacists at Butler CE Symposium, June 2012, Indianapolis, Indiana.

Systematic Review Of Chimpanzee Use In Monoclonal Antibody Research And Drug Development: 1981-2010, Raija Bettauer

Experimentation Collection

This survey examines the extent to which live chimpanzees have been used in monoclonal antibody (mAb) research and the drug approval process. The survey covers 193 scientific articles published during the years 1981-2010, as well as preclinical studies leading to the approval of mAb drugs by the Food and Drug Administration of the United States. The frequency of the articles has decreased by more than two-thirds from their highs in the late 1980’s, and the aggregate number of chimpanzees used in these studies has decreased by more than 90%.

The experimental protocols ranged from single or multiple blood draws to …

Chemical Composition And Product Quality Control Of Turmeric (Curcuma Longa L.), Shiyou Li, Wei Yuan, Guangrui Deng, Ping Wang, Peiying Yang, Bharat Aggarwal

Faculty Publications

Chemical constituents of various tissues of turmeric (Curcuma longa L.) have been extensively investigated. To date, at least 235 compounds, primarily phenolic compounds and terpenoids have been identified from the species, including 22 diarylheptanoids and diarylpentanoids, eight phenylpropene and other phenolic compounds, 68 monoterpenes, 109 sesquiterpenes, five diterpenes, three triterpenoids, four sterols, two alkaloids, and 14 other compounds. Curcuminoids (diarylheptanoids) and essential oils are major bioactive ingredients showing various bioactivities in in vitro and in vivo bioassays. Curcuminoids in turmeric are primarily accumulated in rhizomes. The essential oils from leaves and flowers are usually dominated by monoterpenes while those from …

The College Of Pharmacy, South Dakota State University

Jackrabbits Script and Scope (Formerly called College of Pharmacy and Allied Health Professions Magazine)

Dean’s message (Inside front cover)
[Page] 5 Research Rahman offers Research opportunities to undergraduates
[Page] 7 West River Health Camp Wicozani Academy spotlights native health-care providers
[Page] 9 Education Workshop uses mock patients to develop medical teamwork [Page] 11 Cover Story Avera Health and Science Center lives up to billing
[Page] 14 Administration Students, faculty benefit from new administrative approach
[Page] 15 Building Project Donors Independent pharmacies step forward with gifts
[Page] 17 Alumnus of the Year Alaskan independent pharmacist White honored by College
[Page] 18 Fall Convocation Presentation Diabetes project researches clinical stage
[Page] 19 Distinguished Alum Compounding catapults …

Efficacy And Safety Of Dabigatran: A Comparison Of Food And Drug Administration Approved Dosing Regimens, Kenneth Lupi Pharmd, Joseph Ottinger Rph, Ms, Mba, Bcps

Department of Pharmacy

No abstract provided.

Lessons Learned From Experiences In Academic Leadership, Kenneth Lee Mccall, Gayle A. Brazeau

Pharmacy Practice Faculty Publications

We believe there is nothing better than life and its experiences to mold one’s individual leadership abilities, skills, and talents. We have, like many before us, realized some of these lessons can and will only be achieved with service and leadership activities and responsibilities to our college and to numerous professional educational, practice, and scientific organizations. Three specific lessons learned through personal experiences are offered below which we believe may benefit those who have an interest in academic leadership positions.