Pharmacy and Pharmaceutical Sciences Commons^™

ผลลัพธ์ของการให้บริบาลทางเภสัชกรรมในนักศึกษามหาวิทยาลัยที่ได้รับการรักษาด้วยยาต้านเศร้า, จักรพงค์ มัชฌิมาภิโร

Chulalongkorn University Theses and Dissertations (Chula ETD)

วัตถุประสงค์: วัตถุประสงค์หลักเปรียบเทียบคุณภาพชีวิตด้านการใช้ยาโดยใช้ค่าเฉลี่ยคะแนนจากแบบสอบถาม PROMPT ระหว่างกลุ่มศึกษาและกลุ่มควบคุม วัตถุประสงค์รอง เพื่อเปรียบเทียบความร่วมมือในการใช้ยาและความรู้ด้านยาต้านเศร้าและวิตกกังวล วิธีการวิจัย: การวิจัยเชิงทดลองแบบสุ่มที่มีกลุ่มควบคุมแบบปกปิดทางเดียวในนักศึกษามหาวิทยาลัย ที่ได้รับยาต้านเศร้า ณ ฝ่ายส่งเสริมสุขภาวะนิสิต จุฬาลงกรณ์มหาวิทยาลัย ช่วงระหว่างเดือน เมษายน พ.ศ. 2566 ถึงเดือนกรกฎาคม พ.ศ. 2566 จำนวนทั้งสิ้น 85 คน สุ่มอาสาสมัครเป็น 2 กลุ่ม คือ กลุ่มที่ได้รับการบริการตามมาตรฐาน(กลุ่มควบคุม) และกลุ่มที่ได้รับการบริบาลทางเภสัชกรรมร่วมกับการบริการตามมาตรฐาน (กลุ่มศึกษา) ประเมินผลลัพธ์ด้านคุณภาพชีวิตด้านการใช้ยา ความร่วมมือในการใช้ยาและความรู้ด้านยา ผลการวิจัย: กลุ่มศึกษามีผลลัพธ์ด้านคุณภาพชีวิตด้านการใช้ยา ผลลัพธ์ด้านความร่วมมือในการใช้ยาและผลลัพธ์ด้านความรู้เรื่องยาต้านเศร้าและวิตกกังวลที่ดีขึ้นกว่ากลุ่มควบคุมอย่างมีนัยสำคัญทางสถิติ (p<0.001) สรุปผลการวิจัย: การบริบาลทางเภสัชกรรมร่วมกับการบริการตามมาตรฐานปกติส่งผล เพิ่มผลลัพธ์ด้านคุณภาพชีวิตด้านการใช้ยาทั้ง 8 มิติผลลัพธ์ด้านความร่วมมือในการใช้ยาและ ผลลัพธ์ด้านความรู้เรื่องยาต้านเศร้าและวิตกกังวลดีขึ้นในนักศึกษาที่ได้รับยาต้านเศร้าและวิตกกังวล

Effects Of Cannabidiol On Functions Of Chimeric Antigen Receptor T Cell In Hematologic Malignancies, Natthida Chantarat

Chulalongkorn University Theses and Dissertations (Chula ETD)

CD19-CAR T cell therapy is promising immunotherapy for cancer treatment which has shown remarkable clinical responses, leading to approval by the FDA for relapsed and refractory B-cell hematological malignancies treatment. Cannabidiol (CBD) is a non-psychoactive cannabinoid compound that has been utilized as a palliative treatment in cancer patients due to its immunosuppressive properties. Currently, studies on using CBD during immunotherapy have gained increasing attention. However, the possible interaction between CBD and CAR-T cell therapy has not been studied. Therefore, in this study, we aimed to examine the direct effects of CBD on CD19-CAR T cells’ function against hematologic malignancies. The …

Screening And Identification Of Spore-Forming Bacteria For Lactic Acid Production, Jenjuiree Mahittikon

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research presents the screening and identification of 41 lactic acid-producing bacteria collected from various tree barks in different provinces of Thailand. They belonged to six groups within five genera, including Sporolactobacillus (9 strains), Weizmannia (18 strains), Terrilactibacillus (1 strain), Lactococcus (2 strains), and Enterococcus (11 strains). Based on 16S rRNA gene sequence similarity (98.65%), the novel species in the genera Weizmannia (1 strain), Sporolactobacillus (4 strains), and Enterococcus (1 strain) are found. The lactic acid screening of spore-forming bacteria, S. laevolacticus CUH1-2, exhibited high temperature tolerance and produced D-(-)-lactic acid at high yield and optical purity. Therefore, it was …

Delineating The Impacts Of Race, Aging, And Chronic Kidney Disease On Cytochrome P450 3a And Major Drug Transporters In Thai Population Using Physiologically-Based Pharmacokinetic Model, Udomsak Udomnilobol

Chulalongkorn University Theses and Dissertations (Chula ETD)

Race, aging, and organ impairment are population intrinsic factors that affect drug pharmacokinetic (PK) possibly through changing in function and/or expression of drug metabolizing enzymes and transporters. The impacts of aging and chronic kidney disease (CKD) on CYP3A, but not drug transporters, were demonstrated in Thai population receiving a microdose cocktail containing 5 probe substrates (midazolam, dabigatran etexilate, pitavastatin, rosuvastatin, and atorvastatin). This study therefore aimed to further investigate the impacts of race, aging, and CKD on the drug transporters in Thai subjects, using the physiologically-based pharmacokinetic (PBPK) approach. The population PBPK models were newly developed specifically to 3 groups …

Notch2 Activation Of Full-Length And Truncated Recombinant Jagged1 Fused With Human Igg1 Fc Produced In Mammalian Expression System, Y Tran Nhu

Chulalongkorn University Theses and Dissertations (Chula ETD)

Osteoporosis associated with old ages has emerged in 21st century as an increasing issue. To stimulate the mineral deposition in bone cells, Jagged1 (Jag1) protein has been demonstrated to trigger mineralization via Notch signaling pathway by binding to Notch2 receptor. Therefore, the Jag1 protein is one of promising candidates for osteoporosis treatment. However, Jag1 consisting of 1218 amino acids is infeasible to apply in clinical uses. Here we engineered Jag1 into truncated proteins containing key domains, Delta–Serrate–Lag-2 domain, N-terminal C2 domain and several epidermal growth factor–like domains which could be responsible for the binding of Notch receptor. To ensure the …

The Sequence Dependent Nanoscale Structure Of Cenp-A Nucleosomes, Tommy Stormberg, Yuri L. Lyubchenko

Journal Articles: Pharmaceutical Sciences

CENP-A is a histone variant found in high abundance at the centromere in humans. At the centromere, this histone variant replaces the histone H3 found throughout the bulk chromatin. Additionally, the centromere comprises tandem repeats of α-satellite DNA, which CENP-A nucleosomes assemble upon. However, the effect of the DNA sequence on the nucleosome assembly and centromere formation remains poorly understood. Here, we investigated the structure of nucleosomes assembled with the CENP-A variant using Atomic Force Microscopy. We assembled both CENP-A nucleosomes and H3 nucleosomes on a DNA substrate containing an α-satellite motif and characterized their positioning and wrapping efficiency. We …

Assessing The Accuracy And Deficits Of Popular Drug-Interaction Software Programs In Detecting Interactions Between Cannabis And Pharmaceutical Drugs, Nathan C. Ramsbacher

Undergraduate Theses, Professional Papers, and Capstone Artifacts

- Purpose – The management of drug interactions in healthcare is a large concern for pharmacists and other healthcare professionals, especially for patients requiring large amounts of medications. Healthcare professionals and patients alike utilize drug-interaction software programs (DISPs) frequently to manage medications and make clinical decisions. There is a large discrepancy between DISPs in terms of detecting clinically significant drug interactions. Notably, cannabis does not flag as an interaction as reliably as pharmaceutical drugs on many DISPs. With rising cannabis use in the United States, patients deserve to make decisions on how to safely use cannabis with their prescription drugs …

Building Tools For Improved Modulation Of The Human Gabaa Receptor, A Central Nervous System Target For The Treatment Of Anxiety, Garrett Edward Zinck

Theses and Dissertations--Pharmacy

In the U.S., anxiety is recognized as an increasing range of mentally and physically debilitating psychiatric health disorders with significant economic repercussions. Over the last 20 years, several novel anti-anxiety therapies have entered the drug development pipeline, but none have made it to market.

The work in this dissertation focused on structurally modifying valerenic acid (VA), a structurally unique carboxylated sesquiterpene acid found in Valeriana officinalis. VA is putatively reported to have allosteric modulatory activity of the human GABA_A receptor, a ligand-gated ion channel responsible for attenuating neurotransmissions. Structural modeling of VA’s GABA_A receptor interaction suggests that …

Analysis Of Potential Facilitators To Use Of Hiv Pre-Exposure Prophylaxis (Prep) In A Young Transgender Population, Noah Dixon

Theses and Dissertations--Pharmacy

Pre-exposure prophylaxis for HIV prevention (PrEP) is heavily under-utilized in transgender youth, a population which experiences high risk factors for contracting HIV and exhibits unique barriers to care. This retrospective study used a secondary data analysis of survey results from transgender youth between 16-and 24-years-old to analyze various exposures for association with PrEP use. It was hypothesized that medical gender affirming therapy, mental health care treatment, and HIV programming would be independently associated with increased use of PrEP in transgender youth. It was found that only HIV prevention services and programming related to HIV or gender identity were associated with …

Studies Toward The Development Of An Improved Countermeasure For Synthetic Opioid Overdose, Sidnee L. Hedrick

Theses and Dissertations--Pharmacy

One of the most prominent opioid analgesics in the United States is the high potency agonist fentanyl. It is used in the treatment of acute and chronic pain and as an anesthetic adjuvant. When used inappropriately, however, ingestion of just a few milligrams of fentanyl or other synthetic opioid can cause opioid-induced respiratory depression (OIRD), often leading to death. Currently, the treatment of choice for OIRD is the opioid receptor antagonist naloxone. Recent reports, however, suggest that higher doses or repeated dosing of naloxone (due to recurrence of respiratory depression) may be required to fully reverse fentanyl-induced respiratory depression, rendering …

Investigating The Physical Stability Of Amorphous Pharmaceutical Formulations, Travis W. Jarrells

Theses and Dissertations--Pharmacy

Amorphous formulations, including amorphous solid dispersions (ASDs), consisting of the active pharmaceutical ingredient (API) intimately mixed in a polymeric matrix, are an attractive formulation approach to improve drug delivery, dissolution, and solubility. However, an amorphous API in an ASD is in a higher energy state compared to the crystalline drug and results in most ASDs being inherently unstable. The polymer helps to stabilize the amorphous drug against crystallization such that the resulting homogenous mixture maintains its solubility advantage relative to the crystalline form. One challenge of ASDs is that the presence of impurities including crystals or residual solvent, variations in …

Response Of Dopaminergic System To Cocaine Exposure, Recovery After Cocaine Abstinence, And Impact Of A Long-Acting Cocaine Hydrolase, Jing Deng

Theses and Dissertations--Pharmacy

Cocaine produces its physiological effects by targeting multiple proteins in the central nervous system, particularly by binding with dopamine transporter (DAT) to block the normal dopamine (DA) recycling process. This process along with the pleasure feeling would wear off shortly after the abstinence from the drug. However, chronic cocaine use is associated with neuroadaptations in the dopaminergic system, including increases in the density of DAT on plasma membrane of dopaminergic neurons. Given the critical role of DAT in neurotransmission, various studies have been carried out on animal models to learn its structure, function, and distribution. Long-standing evidence supports that DAT …

Liposomal Technologies To Improve Gene Delivery, David Nardo Padron

Theses and Dissertations--Pharmacy

Lipid based nanoparticles (LBNs) are used in myriad applications in medicine from small molecule drug delivery to mRNA vaccines. A major contributing factor to the development of the field has been the ongoing development of novel compounds that retain the functionality of natural lipids but expand upon them through inclusion of functional moieties that can be applied to specific scientific and biomedical questions. In the body of this dissertation, an extensive overview of LBNs is provided, focusing primarily on their use in immune modulation. The research presented herein begins with the synthesis of a novel class of lipids based on …

The Relative Contribution Of Liver And Intestine In Reverse Cholesterol Transport, Rupinder Kaur

Theses and Dissertations--Pharmacy

Despite decades of advances in research, death due to cardiometabolic disease remains the largest contributor to mortality in the US. While present therapies oppose “forward cholesterol transport,” and atherosclerotic plaque progression, they do not remove cholesterol from established atherosclerotic plaques. Reverse cholesterol transport (RCT) is the active process of mobilizing peripheral cholesterol for excretion through the hepatobiliary (transhepatic cholesterol elimination, THCE) or intestinal (transintestinal cholesterol elimination, TICE) pathways. Currently, there are no clinically approved therapeutics that target RCT. Elucidation of the mechanisms that govern RCT and TICE, and the subsequent identification of novel targets is of importance. The present work …

Development Of Accurate And Efficient Computational Methodologies For Predicting Protein-Ligand And Protein-Protein Binding Free Energies, Alexander Hamilton Williams

Theses and Dissertations--Pharmacy

Computational modeling is an invaluable tool in the drug discovery process either for small ligand or protein therapeutics. The widespread availability of protein X-Ray Crystal and Cryo-Electron Microscopy (Cryo-EM) structures has allowed for more accurate molecular dynamics (MD) simulations that are not reliant on methods such as homology modeling, which may produce structures that require significant computational time to demonstrate their stability. In this thesis we describe several novel methodologies for the computationally efficient modeling of protein/ligand and protein/protein complexes that may be employed within both large-scale virtual screenings and lead compound optimization. These methodologies may also be utilized in …

Development Of Zafirlukast Derivatives Active Against Porphyromonas Gingivalis, Kaitlind C. Howard

Theses and Dissertations--Pharmacy

Periodontal diseases are inflammatory diseases that can lead to damage of the soft tissue and bone supporting the teeth. Gingivitis is the reversible early stage of gum disease, which consist of gums that bleed when brushing or flossing teeth. When left untreated, the preventable but irreversible late stage of periodontal disease, periodontitis, can lead teeth to loosen or even fall out. Oral bacterial species, such as the Gram-negative anaerobic pathogen Porphyromonas gingivalis trigger these inflammatory diseases through oral dysbiosis. P. gingivalis is a keystone pathogen, meaning that the damage caused by P. gingivalis is not proportional to its abundance. Treatment …

Design, Synthesis, Structure Optimization, Structure-Activity Relationship (Sar), And Biological Evaluation Of Novel And Potent Thiazolo[4,5-D]Pyrimidine Corticotropin Releasing Factor (Crf) Receptor Antagonists, Md Rabiul Islam

Electronic Theses and Dissertations

No abstract provided.

Polymeric Nanovaccine Delivery System For Influenza Vaccine, Chaitanya K. Valiveti

Electronic Theses and Dissertations

Vaccines are the most efficient and cost-effective method for preventing illnesses caused by infectious pathogens. Even though the great success of vaccines over decades, the development of safe and robust vaccines is still essential for emerging new pathogens, re-evolving old pathogens, and improving the insufficient protection given by existing vaccines. One of the most critical strategies for developing effective new vaccines is selecting and using a suitable adjuvant or immune stimulant. Immunologic adjuvants are essential for improving vaccine potency by enhancing the immune response of vaccine antigens. The amount of antigen could be spared with improved potency, especially during mass …

Nh125 Sensitizes Staphylococcus Aureus To Cell Wall-Targeting Antibiotics Through The Inhibition Of The Vras Sensor Histidine Kinase, Shrijan Bhattarai, Lane Marsh, Kelsey Knight, Liaqat Ali, Antonio Gomez, Allison Sunderhaus, May H. Abdelaziz

Pharmacy Faculty Publications and Presentations

Staphylococcus aureus utilizes the two-component regulatory system VraSR to receive and relay environmental stress signals, and it is implicated in the development of bacterial resistance to several antibiotics through the upregulation of cell wall synthesis. VraS inhibition was shown to extend or restore the efficacy of several clinically used antibiotics. In this work, we study the enzymatic activity of the VraS intracellular domain (GST-VraS) to determine the kinetic parameters of the ATPase reaction and characterize the inhibition of NH125 under in vitro and microbiological settings. The rate of the autophosphorylation reaction was determined at different GST-VraS concentrations (0.95 to 9.49 …

Modulation Of Ptz-Induced Convulsions In Rats Using Topiramate Alone Or Combined With Low Dose Gamma Irradiation: Involving Akt/M-Tor Pathway, Marwa Safar

Pharmacy

The current study evaluates the anticonvulsant effect low dose whole body gamma irradiation (LDR) alone or combined with topiramate against pentylenetetrazole (PTZ)-induced convulsions. Male Wister rats received either saline or PTZ (75mg/kg i.p.). The other three groups were pretreated with single low dose radiation (0.5Gy), topiramate (50mg/kg, p.o., seven days) and TPM with LDR respectively before PTZ injection. Racine’ score, latency, and duration of the convulsions were assessed. Glutamate and GABA were measured. AKT/m-TOR signaling pathway including AKT (protein kinase B), mammalian target of rapamycin (m-TOR), protein S6, and caspase 3 were also assessed. Measurements of markers of oxidative stress …