Dentistry Commons^™

Anti-Inflammatory And Regeneration Properties Of Iloprost On Inflamed Human Dental Pulp Cells Model: An In Vitro Study, Rahman Wahyudi

Chulalongkorn University Theses and Dissertations (Chula ETD)

In the clinical situation, pulp exposure can happen during caries removal procedures. Inflammation can occur on the pulp of the exposed area because of infiltration of bacteria from caries or as a result of pulp reaction to caries removal drilling. When the pulp was exposed during the caries removal procedure, traditional methods to preserve the pulp such as pulp capping and pulpotomy were performed. The ideal treatment outcome of the pulp exposure is to regain the primary structure of tubular dentin as well as maintain the vitality and healthiness of the dental pulp Thus, good dental materials or drugs used …

Effect Of Shear Force On Immunosuppressive Property Of Human Periodontal Ligament Cells, Ravipha Suwittayarak

Chulalongkorn University Theses and Dissertations (Chula ETD)

The immunomodulatory property is to regulate immune cell function. IDO and TGF-β1 inhibited T cell proliferation and induced regulatory T (Treg) cells differentiation. However, this property must be activated by inflammation. Recently, mechanical forces have also activated this property. Hence, this study investigated effect of shear stress on immunosuppressive property of hPDL cells. Cells were subjected to shear stress then expression of IDO, TGF-β1, IFN-gamma and COX2 mRNA and protein was examined by qRT-PCR, ELISA, and IDO activity. Conditioned media derived shear stress were treated with CD4+ T cells. The proliferative T cell effect was determined using resazurin. The FOXP3, …

Efficacy Of Cannabidiol (Cbd) For The Treatment Of Recurrent Aphthous Ulcers, Chalapinyo Umpreecha

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this research were 1) To investigate the allergic reactions to cannabidiol (CBD) when used on human skin. 2) To assess local and systemic side effects of CBD when used on normal oral mucosa. 3) To compare the efficacy among 0.1% CBD, 0.1% triamcinolone acetonide (TA), and placebo (pure oral paste) in recurrent aphthous ulcer (RAU) treatment. This project was composed of 3 phases. Phase 1, a skin patch test was performed on 100 healthy subjects. Phase 2, CBD was applied to normal oral mucosa of 50 healthy subjects 3 times/day for 7 days. Oral examination, vital signs, …

Effects Of Local Versus Systemic Administration Of Cxcr4 Inhibitor Amd3100 On Orthodontic Tooth Movement In Rats, Narubhorn Ongprakobkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chemokines play pivotal roles in orthodontic tooth movement (OTM) through osteoclast-mediated bone resorption, but the underlying mechanism remains unclear. We aimed to elucidate the effects of serial local vs systemic administration of the chemokine receptor CXCR4 antagonist AMD3100 on OTM. The maxillary right first molar (M1) of rats was moved mesially using a 10 g of force nickel-titanium coil spring. The injections were performed every other day with phosphate-buffered saline as a control, whereas local and systemic animals were injected with AMD3100 at the buccal palatal mucosa adjacent to M1 and subcutaneously, respectively. OTM distance and alveolar bone were examined …

Differences Of Sound And Morphology In Skeletal Class Iii Patients, Natthaporn Pravitharangul

Chulalongkorn University Theses and Dissertations (Chula ETD)

[Background] This study aimed to explore differences in vowel production using acoustic analysis in skeletal Class III and Class I Japanese participants, and to investigate the correlation between vowel sounds and cephalometric variables in skeletal Class III subjects. [Materials and Methods] Japanese males with skeletal Class III (ANB<0°) and Class I skeletal relationships (0.62°

The Designs Of Miniscrew-Assisted Transpalatal Arch Affect Molar Intrusion, Ornnicha Pooktuantong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Abstract: An anterior open bite is one of the most difficult malocclusions in orthodontic treatment. For such malocclusion, orthodontic miniscrews insertion into both buccal and palatal alveolar regions has been indicated for molar intrusion, but it involves a risk of tooth root injury. To solve the problem, midpalatal miniscrew-attached extension arm (MMEA) is adopted. However, this method causes palatal tipping of the molar because intrusive loads were applied only from palatal side. Currently, transpalatal arch is added to avoid tipping movement, but it induces patient's discomfort. Hence, the objective of this study was to evaluate the loading conditions for maxillary …

Effects Of Intrauterine Intermittent Hypoxia On Skeletal Muscle In Offspring Rats, Wirongrong Wongkitikamjorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Gestational intermittent hypoxia (IH), a hallmark of OSA, alters the offspring's respiratory neural control and diaphragm contractile function. Thus, we aimed to investigate the effects of gestational IH on the muscle development and metabolism of geniohyoid (GH), which is innervated by the respiratory-related hypoglossal nerve and plays a role in tongue traction and suckling, in male offspring rats compared with masseter (MAS), the largest masticatory muscle. Pregnant Sprague-Dawley rats were exposed to IH (3-min periods of 4–21% O2) compared to Normoxia for 8 hours/day during gestational days 7–20. GH and MAS from 35-day-old male offspring (n = 6 /group) were …

Cellular Responses Of Stem Cells Isolated From Human Exfoliated Deciduous Teeth Towards Different Concentration Of Calcium Ion, Thanika Phlinyos

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: The purpose of this study was to investigate the effect of Ca2+ on proliferation, osteogenic differentiation, and migration of stem cells from human exfoliated teeth (SHEDs) in vitro Materials and methods: SHEDs were seeded in culture media and osteogenic induction media containing 1.8-16.2 mM of Ca2+. SHEDs proliferation was determined using MTT assay and colony forming unit assay. Osteogenic differentiation was evaluated using mineralization assay and osteogenic marker gene expression and cell migration was evaluated using wound healing assay. Values were expressed as mean + S.D. Statistical analysis of MTT assay and wound healing assay were performed using two-way …

Expression Of Vascular Endothelial Growth Factor Protein In Mrna-Transfected Human Periodontal Ligament Cells, Mana Naratippakorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

The complete regeneration of periodontal tissues following current periodontal therapy remain challenging and unpredictable. Nucleoside-modified messenger RNA (mRNA) technology can be a promising novel platform in regenerative medicine. The aims of this study were to evaluate whether pseudouridine modified mRNA encoding vascular endothelial growth factor (VEGF) could induce VEGF production in human periodontal ligament cells (PDLCs) and this translated protein function by promoting in vivo blood vessel formation using chorioallantoic membrane (CAM) assay. Isolated PDLCs from healthy periodontal tissue were transfected with modified mRNA encoding VEGF (VEGF mRNA) complexed with a transfecting agent, Lipofectamine 2000 (L2000) and L2000 alone (control). …

The Efficacy Of Universal Ceramic Polishing Kit On The Surface Roughness Of Various Ceramic Types, Ekaluck Poosanthanasarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objective of this vitro study was to determine the effect of polishing performance of a universal ceramic polishing kit on the surface roughness of various ceramics. The ceramic specimen size 5 x 7 x 4 mm of lithium disilicate glass ceramic (IPS e.max CAD, Ivoclar Vivadent, Liechtenstein), translucent zirconia (VITA YZ XT, VITA Zahnfabrik, Germany) and zirconia-reinforced lithium silicate (Celtra Duo, Dentsply Sirona, United States) (n=8) were fixed with clear resin in PVC block, 14 mm in diameter. The specimens were ground with fine diamond bur for 15 seconds to simulate clinical gross contouring.The two-step polishing process started with …

The Effects Of The Oral Exercises On The Oral Function Of People With Dementia, Kwanrutai Somsak

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objective of this study was to examine the effects of the home-based oral exercises on the oral function in people with mild to moderate dementia. The patients with mild to moderate dementia at King Chulalongkorn Memorial Hospital, who were randomly allocated to an experimental group (n = 11), were asked to perform 3 home-based oral exercises for 3 months while those in a control group (n = 11) did not do any oral exercises. The oral function including maximum tongue pressure (MTP), oral diadochokinesis (ODK) rates of /ta/, /ka/, /pa/, swallowing function (modified water swallowing test: MWST), and oral …

Antifungal, Physical, And Mechanical Properties Of Tissue Conditioner Incorporated With Zinc Oxide Nanoparticles, Woraporn Homsiang

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study evaluated the antifungal, physical, and mechanical properties of tissue conditioner incorporated with different amounts of zinc oxide nanoparticles (ZnOnps) at different storage times (0, 7, and 14 days). Specimens of 0, 5, 10, 15 wt% ZnOnps, or 15 wt% nystatin incorporated into tissue conditioner were fabricated. The direct contact test (n=6) was performed to evaluate the antifungal effect against C. albicans suspension. Scanning electron microscopy and energy dispersive X-ray spectroscopy (n=6) were used to observe the amount and distribution of superficial zinc element. The penetration depth (n=6) and tensile bond strength (n=8) were evaluated following ISO 13139. The …

การศึกษาผลของวัสดุบูรณะที่ปลดปล่อยแร่ธาตุต่อการคืนแร่ธาตุในรอยผุระยะเริ่มต้น ของฟันกรามน้ำนมซี่ข้างเคียงในห้องปฏิบัติการ, ดนุธิดา สาเขตร์

Chulalongkorn University Theses and Dissertations (Chula ETD)

การศึกษาในห้องปฏิบัติการนี้มีวัตถุประสงค์เพื่อเปรียบเทียบการคืนแร่ธาตุในรอยผุระยะเริ่มต้นของชิ้นฟันที่สัมผัสกับวัสดุบูรณะคลาสทูด้วยนาโนคอมโพสิตแคลเซียมฟอสเฟต (Predicta™ bioactive bulk) กับซีเมนต์กลาสส์ไอโอโนเมอร์ (Equia Forte®) และเรซินคอมโพสิตชนิดไม่ปลดปล่อยฟลูออไรด์ (Filtek™ Z350) เป็นกลุ่มควบคุม โดยใช้ชิ้นฟันน้ำนมจำนวน 60 ชิ้นจากด้านแก้มของฟันกรามน้ำนมที่ถูกถอน 60 ซี่มาสร้างรอยผุระยะเริ่มต้นที่กึ่งกลางของชิ้นฟัน นำชิ้นฟันตัวอย่างมาวัดร้อยละการสูญเสียฟลูออเรสเซนต์ (∆F) เริ่มต้นด้วยเครื่องวัดฟลูออเรสเซนต์ที่เกิดจากแสงเชิงปริมาณชนิดดิจิทัล เรียงลำดับร้อยละการสูญเสียฟลูออเรสเซนต์เริ่มต้นจากชิ้นฟันที่สูญเสียแร่ธาตุน้อยไปมาก สุ่มชิ้นฟันออกเป็น 3 กลุ่ม นำชิ้นฟันไปติดกับบล็อกอะคริลิกที่บูรณะด้านประชิดด้วยวัสดุบูรณะทั้ง 3 ชนิด แล้วเข้ากระบวนการเลียนแบบสภาวะในช่องปากเป็นเวลา 14 วัน จากนั้นวิเคราะห์ปริมาณการคืนแร่ธาตุโดยวัสดุบูรณะแต่ละชนิด ด้วยการเปรียบเทียบค่าเฉลี่ยของการเปลี่ยนแปลงร้อยละของการสูญเสียฟลูออเรสเซนต์ (∆∆F) และค่าเฉลี่ยของการเปลี่ยนแปลงปริมาตรการสูญเสียฟลูออเรสเซนต์ (∆∆Q) ผลการศึกษาพบว่าทั้งค่า ∆∆F และค่า ∆∆Q ของทุกกลุ่มมีความแตกต่างอย่างมีนัยสำคัญทางสถิติ โดยกลุ่มซีเมนต์กลาสส์ไอโอโนเมอร์ มีการคืนแร่ธาตุแก่ชิ้นฟันสูงที่สุด ส่วนกลุ่มนาโนคอมโพสิตแคลเซียมฟอสเฟตมีการสูญเสียแร่ธาตุของชิ้นฟันเล็กน้อย ซึ่งน้อยกว่ากลุ่มควบคุม คือเรซินคอมโพสิตชนิดไม่ปลดปล่อยฟลูออไรด์อย่างมีนัยสำคัญทางสถิติ สรุปได้ว่าการบูรณะด้วยวัสดุซีเมนต์กลาสไอโอโนเมอร์ สามารถคืนแร่ธาตุให้ฟันซี่ข้างเคียงได้ดีที่สุด แต่ในกรณีที่รอยผุหรือโพรงฟันคลาสทูมีขนาดใหญ่ นาโนคอมโพสิตแคลเซียมฟอสเฟตก็ถือเป็นวัสดุหนึ่งที่เป็นทางเลือกในการใช้ทดแทนเรซินคอมโพสิตชนิดไม่ปลดปล่อยฟลูออไรด์ เนื่องจากใช้งานง่าย มีความแข็งแรงและความสวยงามเทียบเท่ากับเรซินคอมโพสิต นอกจากนี้ยังช่วยยับยั้งการลุกลามของรอยผุระยะเริ่มต้นในฟันซี่ข้างเคียงได้ดีกว่าเรซินคอมโพสิตชนิดไม่ปลดปล่อยฟลูออไรด์

Influence Of Training Schedule On Learning Outcome Of Motor Skills For Dynamic Navigation System In Esthetic Zone, Wirawat Kunakornsawat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Backgroud: Currently, a novel dynamic navigation system has been demonstrated to improve the accuracy of 3D implant placement, but proficiency in this system is quite difficult to achieve. Thus, the training course for using this system is required. The distributed training has been shown to be superior to massed training, it is still uncertain whether differences in distributed training rest periods will result in the best motor skill acquisition for dynamic navigation system. Purpose: The objective of this study was to determine the most effective schedule for training motor skill needed to perform implant placement using dynamic navigation system: distributed …

Finite Element Analysis Of Customized Dental Implant In Different Bone Quality, Pawhat Nimmawitt

Chulalongkorn University Theses and Dissertations (Chula ETD)

Purpose: The purpose of this finite element analysis (FEA) study was to compare stress distribution among three different designs of a novel 3D printed root analog dental implant and a conventional screw-type implant, for different types of bone density. Materials and Methods: A mandibular right second premolar was selected from CBCT database. Four models were created: 1)Solid root analog implant 2)Solid&Porous root analog implant 3)Porous root analog implant and 4)Screw-type implant (control). Bone quality types I-IV was determined based on Lekholm and Zarb classification. Von Mises stress was measured at bone and implant. Results: FEA models were developed and von …

Retentive Force Change Of Nylon Insert In Locator R-Tx Attachment After Brushing Test, Wasita Pongwattana

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to evaluate the effect of brushing on the percentage change of retentive force in Locator R-Tx attachment. There were 3 types of nylon inserts; blue, pink, and clear. The nylon inserts were brushed in 2 media; water and toothpaste. Each group had 6 specimens (n=6). All specimens were soaked in deionized water at 37 ºC for 7 days, after that they were placed in the simulated brushing machine. Before and after they underwent 5,000 brushing cycles, the retention test of each specimen was measured with a universal testing machine. The retentive forces were repeatedly measured 5 times …

Comparison Of Esthetic Perception Of Maxillary Central And Lateral Incisors Between Dentists And Laypersons, Pastharee Jarusripat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: To compare esthetic perception between dentists and laypersons by difference of asymmetrical width and length of maxillary central and lateral incisors under 5 and 15 seconds evaluation time. Materials and Methods: Photos of women were digitally altered by Adobe Photoshop® program to create image with symmetrical and asymmetrical tooth width or length in 0.5 mm increments. Final images were randomly present on computer screen using an equal ratio to actual tooth. Thirty-six dentists and 36 laypersons were asked to rate attractiveness of those smiles on a numerical rating scale within 5 and 15 seconds of evaluation time. Mann-Whitney U …

Effects Of Small Molecules In Wnt Signaling On Human Dental Pulp Cells, Chatvadee Kornsuthisopon

Chulalongkorn University Theses and Dissertations (Chula ETD)

Osteoblast differentiation requires interaction of several cell signaling to coordinately modulate cell responses. Understanding the integration between signaling pathways provides an insight into molecular mechanism that governs stem cell differentiation. Notch, Wnt, and Interleukin15 (IL15) signaling are among the crucial pathways that control biological processes including osteogenic differentiation. Therefore, this dissertation was focused on modulating these signaling pathways in human dental pulp stem cells (hDPSCs). First, the roles of Wnt signaling in dental pulp homeostasis and dentin regeneration were reviewed. Previous evidence suggested that Wnt might be a promising target cell signaling for dentin regeneration. Thus, effect of Tideglusib, a …

Amixicile, A Novel Antimicrobial Agent, And Its Effect On The Salivary And Subgingival Microbiome In Rhesus Macaque Monkeys, Erin E. Block

Theses and Dissertations

Background/Purpose: Periodontitis is an inflammatory disease with a bacterial etiology in a susceptible host. A selective antibiotic with minimal systemic side effects could be a useful adjunct to traditional periodontal therapy. Amixicile is a novel antimicrobial agent that targets pyruvate: ferredoxin oxidoreductase (PFOR), an enzyme that is critical for anaerobic bacterial metabolism and has been found to have little-to-no side-effects in animal models. The aim of this study was to prospectively evaluate the effect of amixicile on clinical periodontal parameters and the composition of the oral microbiome in rhesus macaque monkeys. It was hypothesized that the amixicile would reduce growth …

Antibiotic-Loaded Dendrimer Hydrogels In Periodontal Bone Regeneration: A Feasibility Study, Nicholas G. Yesbeck

Theses and Dissertations

Purpose: Prescription of a complete course of oral antibiotics following bone grafting procedures is a common clinical practice in surgical periodontics. Prophylactic administration of antibiotics reduces the risk of surgical site infection and systemic administration is preferred over local delivery because of short duration of action in the latter circumstance. The goal of this study is to demonstrate the ability of dendrimer hydrogels (DH) to prolong the release kinetics of antibiotics in-vitro. A secondary goal will be to analyze the effect of different particulate bone allografts on the release kinetics of the antibiotic.

Methods: Dendrimer hydrogels (DH) were synthesized …