Probing The Porphyromonas Gingivalis Nitrosative Stress Response Network, Alexandra Galina

Theses and Dissertations

Objective: To persist in the oral cavity, the perio-pathogen Porphyromonas gingivalis must have an efficient nitrosative stress response mechanism. To better understand how P. gingivalis responds to nitrite (NO₂^-) we must determine what genes are involved in the nitrosative stress response network.

Methods: Through genomic screening, a list of genes was generated that were hypothesized to be part of the nitrosative stress response network and believed to be involved in nitrate/nitrite metabolism or redox state of the cells. Protein coding regions were replaced with the ermF sequence by allelic exchange mutagenesis. 15 mutants were created …

Comparison Of Skeletal Class Iii Malocclusion Facial-Shape Subtypes Between Caucasian, African American, And Hispanic Populations, Rachel Katherine Huffman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Comparison of Skeletal Class III Malocclusion Facial-Shape Subtypes Between

Caucasian, African American, and Hispanic Populations

Rachel K. Huffman, DMD

Introduction: The development of a skeletal Class III malocclusion (Cl III) is complex as it can arise from the overgrowth of the mandible, undergrowth of the maxilla, or a combination of the two. To understand the heterogeneity of this trait clinically, studies have been conducted using facial shape analysis (e.g., Geometric morphometrics (GeoMorph)) of Caucasian and Asian populations to learn more about the principal shapes. Little is known, however, about the predominant principal shape components of Cl III within African …

A Comparison Of Endodontic Root-End Filling Techniques: A Micro-Computed Tomography Analysis, Leslie P. Wolford

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction:

Researchers have compared the differences between hand compaction of root-end filling bioceramics with compaction of these materials by hand followed by ultrasonic activation (hand/ultrasonic compaction). Additionally, most of these researchers did not utilize Micro-Computed Tomography (Micro-CT) analysis. The purpose of this study is to evaluate total porosity volume of three different bioceramics when compacted by hand alone versus hand/ultrasonic compaction using Micro-CT Analysis.

Materials and methods:

Seventy-two human maxillary central incisors were selected for the study. All were subjected to orthograde endodontic treatment followed by root tip resection 3-mm from the apex at a 90° to the long axis …

A Comparison Of The Failure Rate Of Two Orthodontic Light-Curable Adhesives: A Chart Review, Ian Montgomery Weaver

Graduate Theses, Dissertations, and Problem Reports (ETD)

A Comparison of the Failure Rate of Two Orthodontic Light-Curable Adhesives: A Chart Review

Ian Montgomery Weaver, D.D.S.

Background and Objectives: The overhead costs of running a private orthodontic practice are infamously high, thus there is a notable benefit to finding the most cost effective methods of treating patients while still maintaining high quality care. A relatively new light-cure orthodontic adhesive BracePaste®^® (American Orthodontics™, Sheboygan, WI), introduced to the market in 2017, can fluoresce under UV light to assist in identifying the difference between the enamel of the tooth and the adhesive material itself, an ability which can aid …

Pinch Force Generation During Scaling By Dental Professionals: A Systematic Review, Jessica R. Suedbeck, Emily A. Ludwig

Dental Hygiene Faculty Publications

Objective

The objective of this review was to examine the impact of instrument designs on pinch force generation during scaling by dental professionals.

Methods

Three databases were utilized from September 2019 to November 2021 in addition to hand-searching specific journals and reference lists. Research articles that examined pinch force generation in dental professionals during scaling with manual instruments only were included. Bias was assessed in the individual articles.

Results

Six research articles were included with sample populations that varied from 12 to 24 participants. Four articles evaluated instrument designs in relation to pinch force generation during scaling by dental professionals. …

Health Profession Students' Interprofessional Socialization And Values Toward A College-Based Virtual Interprofessional Education Activity, Denise Claiborne, Praveen Durgampudi, Anne C. Dumadag, Emily J. Hawkins, Patricia Laverdure, Amy Laurie Meeker, Barbara Kraj, Angela Wilson, Muge Akpinar-Elci

Dental Hygiene Faculty Publications

Purpose: The inclusion of interprofessional education (IPE) is essential for the health professions curricula and establishes the expectation of collaborative patient-centered care. The COVID-19 pandemic has required educators to develop innovative methods for IPE student engagement. The purpose of this study was to evaluate a college-based virtual IPE activity for first-year health profession students and measure their interprofessional socialization and values (ISVS) toward IPE teams. The validated interprofessional socialization and valuing scales (ISVS-9A/9B) were used.

Methods: A one-group pre-post-test design examined health profession students' ISVS from seven health programs before and after a virtual IPE activity. The IPE activity was …

The Effects Of E-Cigarette Aerosol On The Force Degradation Of Elastomeric Chain: An In Vitro Pilot Study, Nicholas David Palmer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background and Objectives

An ideal orthodontic force system should exert continuous light force. An orthodontist has the option to utilize different systems for closing orthodontic extraction space. A commonly used method when closing extraction space is to utilize orthodontic elastomeric chain. Energy Chain® (Rocky Mountain Orthodontics, Franklin, IN) allows for less deformation of shape following the initial stretch, more consistent force, and less force degradation over time. The mouth is a harsh environment that experiences daily changes in pH and temperature. These changes cause elastomeric chain to experience increased force degradation. The creation of the JUUL® (JUUL Labs, San Francisco, …

Pediatric Oral Health: Advances And Challenges, Denise M. Claiborne

Dental Hygiene Faculty Publications

No abstract provided.

Population Variations Of Cheiloscopy Patterns: A Cross-Sectional Observation Pilot Study, Emily Smith Regan, Brenda T. Bradshaw, Ann M. Bruhn, Walter Melvin, Sinjini Sikdar

Dental Hygiene Faculty Publications

Purpose Lip prints are unique and have potential for use as a human identifier. The purpose of this study was to observe possible cheiloscopy differences of individuals with and without parafunctional oral habits such as smoking, vaping, playing a wind instrument or using an asthma inhaler.

Methods This IRB approved blinded cross-sectional observation pilot study collected lip prints from sixty-six individuals, three of which were excluded. Participants cleansed their lips, then lipstick was applied to the vermillion zones of the upper and lower lips. Adhesive tape was applied to the lips and prints were transferred to white bond paper for …

Dental Hygiene Students' Matching Accuracy When Comparing Antemortem Dental Radiographs And Oral Photographs To Simulated Postmortem Winid3® Odontograms, Brenda T. Bradshaw, Amber W. Hunt, Emily Ludwig, Tara L. Newcomb

Dental Hygiene Faculty Publications

Matching dental antemortem (AM) and postmortem (PM) data for human identification is especially challenging when the workforce is limited. Dental hygienists have served mass fatality incidents (MFIs) due to dental-related expertise. However, forensics within dental hygiene education and research on transferable skills is limited. This qualitative balance design study assessed senior dental hygiene students' match accuracy of simulated cases varying in dental identifiers based on AM full mouth series (FMS) radiographs and oral photographs to PM WinID3® odontograms to demonstrate possible disaster victim identification (DVI) transferable skills gained during formal education. A convenience sample of senior dental hygiene students ( …

Colour-Blind Racial Attitudes In Entry-Level Dental Hygiene Students In Virginia, Emily Ludwig, Jessica Suedbeck

Dental Hygiene Faculty Publications

Objective: Subscribing to colour-blind racial attitudes may contribute to inequities in the delivery of oral care and affect treatment of diverse patients. The purpose of this study was to survey all entry-level dental hygiene students in one state to determine colour-blind racial attitudes.

Methods: After IRB approval, a convenience sample of 219 dental hygiene students in all entry-level programmes in Virginia were invited to participate in this cross-sectional study. The Colour-Blind Racial Attitudes Scale (CoBRAS), an effective, validated measuring instrument, was used to quantify unawareness of contemporary racist ideals. Three subscales (Racial Privilege, Institutional Discrimination and Blatant Racial Issues) were …

Dental Hygiene And Dental Assistant Students' Simulated Dvi Radiographic Match Accuracy: A Pilot Study, Samantha C. Vest, Brenda T. Bradshaw, Marsha A. Voelker, Ann M. Bruhn, Tara L. Newcomb, Sinjini Sikdar

Dental Hygiene Faculty Publications

Purpose Allied dental health care professionals have served on disaster victim identification (DVI) teams; however, the literature is void of statistical measures regarding transferable skills and disaster preparedness. The purpose of this study was to assess second year dental hygiene and dental assistant students’ match accuracy for simulated DVI radiographs and compare the match accuracy between the student groups.

Methods Five patient cases were chosen at random to retrospectively collect sets of digital bitewing radiographs from two time periods. The five retrospectively selected sets of images served as simulated antemortem (AM) and postmortem (PM) radiographs. A convenience sample of second …

Cheiloscopy Patterns In Individuals With And Without Parafunctional Oral Habits: A Cross-Sectional Observation Pilot Study, Emily Regan, Brenda Bradshaw, Ann Bruhn, Walter Melvin, Sinjini Sikdar

Dental Hygiene Faculty Publications

Purpose

Lip prints are unique and have potential for use as a human identifier. The purpose of this study was to observe possible cheiloscopy differences of individuals with and without parafunctional oral habits such as smoking, vaping, playing a wind instrument or using an asthma inhaler.

Methods

This IRB approved blinded cross-sectional observation pilot study collected lip prints from 66 individuals, three of which were excluded. Participants cleansed their lips, then lipstick was applied to the vermillion zones of the upper and lower lips. Adhesive tape was applied to the lips and prints were transferred to white bond paper for …

The Relationship Between The Expression Of Stromal Cell Derived Factor-1 (Sdf-1) And Regional Acceleratory Phenomenon (Rap) In Orthodontic Tooth Movement After Tooth Extraction In Rats Model, Duangtawan Rintanalert

Chulalongkorn University Theses and Dissertations (Chula ETD)

The stromal cell-derived factor 1 (SDF-1)/chemokine receptor type 4 (CXCR4) axis plays a key role in alveolar bone metabolism during orthodontic tooth movement (OTM). Herein, the effects of the SDF-1/CXCR4 axis on the regional acceleratory phenomenon (RAP) in OTM velocity and on changes in the surrounding periodontium after adjacent tooth extraction in rats were investigated. Six-week-old male Wistar/ST rats underwent left maxillary first molar (M1) extraction and mesial OTM of the left maxillary second molar (M2) with a 10-g force closed-coil spring. Phosphate-buffered saline, immunoglobulin G (IgG) isotype control antibody, or anti-SDF-1 neutralizing monoclonal antibody were injected at the M1 …

Effects Of ⍺-Lactalbumin Hydrolysate On Human Oral Squamous Cell Carcinoma Cell Lines, Agustina Putri Kintan Arini

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background : Alpha-lactalbumin, the protein from human and bovine milk has been found to be promising as an alternative of anticancer agent Objective: To investigate the effects of lactalbumin enzymatic hydrolysate (LAH) on cell proliferation, migration, and mRNA expression of MMP on human squamous cell carcinoma (hSCC) cell lines, in vitro. Materials and Methods: Tongue (HSC-4 and 7) and pharyngeal (HN-30 and 31) hSCC cell lines were treated with a two-fold dilution of LAH (0.39-100 mg/ml). Cell viability and cell proliferation were examined by MTT assay. Cell migration was investigated by a scratch wound healing assay. Gene expression of metastasis-associated …

Dental And Oral Manifestations Of Rheumatoid Arthritis: Is It Related To General Disease Activity?, Omnia A. Abubakr, Ali M Youssef, Mohamed A Mohamed, Mohamed A Hamdy

Journal of Medicine in Scientific Research

Background: RA is a chronic immune-mediated systemic disorder; its pathogenesis is complex. Genetic, environmental, hormonal, and lifestyle are the main risk factors. The correlation between oral health and RA is rising. Oral manifestations in RA include temporomandibular joint disorder, secondary Sjögren’s syndrome, xerostomia, and periodontal disease. Previous studies suggest a bidirectional relationship between RA and periodontal diseases. This study aims to detect oral mucosal and dental disease in RA patients and its relation to demographic features, disease activity, and extra-articular manifestation. Patients and Methods: This cross-sectional study included 150 patients with RA allocated into two groups, 75 patients in each …

Oral Mucosal And Dental Affection In Patients With Systemic Lupus Erythematosus: A Clinical Survey, Omnia A Abubakr, Ali M Youssef, Mohamed A Mohamed, Mohamed A Hamdy

Journal of Medicine in Scientific Research

Background: SLE is a chronic autoimmune disease predominantly affecting young females. Systemic involvement along with variable clinical presentations characterizes SLE. Various oral and dental manifestations have been described in SLE that include oral ulcerations, candida infection, and periodontal disease. We aimed to detect oral mucosal and dental disease in SLE patients and its relation to demographic features, general disease activity, and the extra-articular manifestation. Patients and Methods: 80 patients with SLE were included in this cross-sectional study. Patients were allocated into 2 groups, 40 patients in each group, according to SLE disease control with immunosuppressive medications: group A included patients …

Enhanced Human Periodontal Ligament Stem Cell Viability And Osteogenic Differentiation On Two Implant Materials: An Experimental In Vitro Study, Engy Adel Ahmed Farag, Sara Fikry Dr, Shereen N. Raafat

Dentistry

Background: Surface roughness of dental implants impacts the survival of adult periodontal stem cells and rate of differentiation. This research was conducted to test how human periodontal ligament stem cells behaved on yttria stabilized tetragonal zirconia polycrystals and polyetheretherketone (PEEK) discs with different surface topographies.

Methods: Discs roughening was prepared by sandblasting. Stem cells were cultivated on zirconia discs with a polished surface, polyetheretherketone (PEEK) discs with a polished surface, sandblasted zirconia discs and sandblasted polyetheretherketone (PEEK) discs. Cells viability was assessed after 24, 48, 72 hours. Scanning electron microscopy was used to examine the adherence and attachment of cells. …

Comorbidity Of Hypertension And Chronic Renal Failure And Their Impact On Oral Complications: A Cross-Sectional Study., Dalia Ghalwash, Sara Abdelwahed, Asmaa Abobakr

Dentistry

The present study aimed to investigate the influence of both end-stage renal diseases (ESRD) and hypertension (HTN) on the various oral manifestations and periodontal condition, to evaluate and compare the oral manifestations and periodontal health status in hypertensive patients with ESRD versus control, and to explore any possible correlation of the oral findings with systemic disease status. This was a cross-sectional study which included two equal groups of patients, Group I were hypertensive patients with ESRD and Group II were hypertensive patients only. All patients were hypertensive and were on antihypertensive medications. Regarding the incidence of oral lesions in both …

Effect Of Overlay Material Type And Design On Fracture Resistance Of Mod Cavities In Molars. (In-Vitro Study), Sara Adel Botros, Mohamed Fouad Haridy, Shaimaa Gad

Dentistry

Objective: To evaluate the influence of restoration material type (CAD/CAM lithium disilicate blocks and reinforced composite blocks) and restoration design on fracture resistance of molars with MOD cavities. Methods and Materials: A total of 54 sound human molars were divided into 2 groups (n=9) according to: 1) material type: lithium disilicate CAD/CAM blocks and reinforced composite CAD/CAM blocks; 2) restoration design: overlay with intra-coronal extension or without intra-coronal extension. Additional 18 samples were assigned to positive control group (sound molars, n=9) and negative control group (unrestored MOD cavities, n=9). Standardized MOD cavities were prepared with occlusal width 3 mm, cavity …