Improvement Of Forage Quality By Means Of Biotechnology: Stable Transformation Of Warm-Season Grasses By Particle Bombardment,
2024
Miyazaki University, Japan
Improvement Of Forage Quality By Means Of Biotechnology: Stable Transformation Of Warm-Season Grasses By Particle Bombardment, C Yuge, R Akashi, O Kawamura
IGC Proceedings (1977-2023)
We have used a simple and inexpensive, self-built particle acceleration apparatus for direct delivery of DNA to cultured cells of warm-season grasses. High levels of transient expression of the b-glucuronidase gene were obtained following bombardment of embryogenic suspension cells and calli of bahiagrass (Paspalum notatum Flügge) and dallisgrass (Paspalum dilatatum Poir). Furthermore, stable transformed calli of both species have been obtained using this simple particle gun.
Transformation In Lotus Corniculatus: Towards Low-Lignin Pasture Through Antisense Rna,
2024
Miyazaki University
Transformation In Lotus Corniculatus: Towards Low-Lignin Pasture Through Antisense Rna, R Akashi, T Uchiyama, Y Sakatani, O Kawamura
IGC Proceedings (1977-2023)
We have developed a rapid and reproducible transformation system for bird’s-foot trefoil (Lotus corniculatus L.) by using Agrobacterium-mediated T-DNA transfer and the incorporation of the antisense gene for cinnamyl alcohol dehydrogenase (CAD) from Aralia cordata into Lotus for lignin reduction. The presence of the transferred antisense gene in regenerated plants has been confirmed by PCR analysis.
Possibility Of Selection For Mineral Concentration In Orchardgrass By X-Ray Microanalysis,
2024
Iwate University
Possibility Of Selection For Mineral Concentration In Orchardgrass By X-Ray Microanalysis, S Saiga, Y Nishimura, K Izumi
IGC Proceedings (1977-2023)
To assess the ability of X-ray microanalysis for screening breeding populations of orchardgrass in mineral concentration, correlations between chemical and X-ray analysis were investigated. Six orchardgrass plants with high- and low-Mg concentrations were examined for three harvest dates in 1995. The samples were separated into leaf blade, leaf sheath, culm and head. Mg concentration in leaf and head were higher than in sheath and in culm. P concentration was highest in the head, and lowest in the sheath. Correlations between chemical and X-ray analysis were r=0.692***, 0.526***, 0.252* and 0.562*** for Mg, Ca, K and P, respectively. Correlation coefficients between …
Aluminum Tolerance Qtl In Diploid Alfalfa,
2024
University of Georgia
Aluminum Tolerance Qtl In Diploid Alfalfa, M K. Sledge, J H. Bouton, J Tamulonis, W A. Parrot, G Kochert
IGC Proceedings (1977-2023)
Aluminum (Al) toxicity associated with acid soils greatly inhibits alfalfa (Medicago sativa L.) productivity throughout much of the world’s major grassland areas. In this paper, we report the identification of quantitative trait loci (QTL) controlling aluminum tolerance in diploid alfalfa (Medicago sativa L). An in vitro callus growth bioassay was used to select aluminum tolerant and aluminum sensitive parents, and to screen an F2 population for aluminum tolerance. Fifty-eight cDNA probes were mapped to nine linkage groups, and the F2 genotypic classes were contrasted with means from the callus growth bioassay using ANOVA. We also used Mapmaker-QTL to …
Selecting Acid-Soil Tolerant White Clover,
2024
USDA, Agricultural Research Service
Selecting Acid-Soil Tolerant White Clover, P W. Voigt, D R. Morris
IGC Proceedings (1977-2023)
A two-stage, tandem, recurrent-selection program is proposed to improve acid-soil tolerance of white clover, Trifolium repens L. In stage one, we evaluate root growth of very young seedlings, grown in an highly-acid Al toxic soil. Although the relationship between the acid-soil response of very young seedlings and that of mature white clover is unknown, the failure of white clover to become established on a highly-acid, Al-toxic soil suggests that establishment is a critical stage of plant development on such soils. In stage two we reevaluate genotypes selected in stage one, in a small-pot study at two lime levels, to separate …
Lodging Resistance Evaluation In Lolium Multiflorum,
2024
Hokuriku Agricultural Experiment Station, Japan
Lodging Resistance Evaluation In Lolium Multiflorum, Katsuo Egara, Kazuhiro Tase, Makoto Kobayashi, Hiroki Fujii
IGC Proceedings (1977-2023)
The objective of this study was to develop a simple technique to evaluate lodging resistance in Italian ryegrass (Lolium multiflorum) for genetic improvement. Significant relationships were recognized between the pushing resistance index and actual lodging degree (ALD), and between the breaking resistance index and ALD, at the 0.1 and 5 percent levels, respectively.
Mechanistic Modelling Of Grassland Energy Balance,
2024
Lincoln University, New Zealand
Mechanistic Modelling Of Grassland Energy Balance, G D. Buchan, B Main, B A. Mckenzie
IGC Proceedings (1977-2023)
No abstract provided.
Radiation Balance And Production Of Forage Crops In A Mediterranean Region,
2024
Instituto Sperimentale Agronomico, Italy
Radiation Balance And Production Of Forage Crops In A Mediterranean Region, G Rana, F Fornaro
IGC Proceedings (1977-2023)
This study is devoted to measure the radiation use efficiency (RUE) and the extinction light coefficient (k) of a sainfoin crop, cultivated in a site of Southern Italy. The same parameters are measured also for the global solar radiation (in this case kg and RUEg, respectively). The crop was maintened in well watered conditions and the measures were carried out during four cut cycles, one during 1994 and three during 1995. Transmitted, incident, soil and canopy reflected radiations were measured continously during the growth season. The results were given a mean of 2.61 and 1.2 …
Use Of Biomass From Reed Canary Grass (Phalaris Arundinacea) As Raw Material For Production Of Paper Pulp And Fuel,
2024
Svalof Weibull, Sweden
Use Of Biomass From Reed Canary Grass (Phalaris Arundinacea) As Raw Material For Production Of Paper Pulp And Fuel, B Andersson, E Lindvall
IGC Proceedings (1977-2023)
Reed canary grass (Phalaris arundinacea) has been investigated as a potential industrial crop in Sweden and other European countries. Reed canary grass (RCG) can be used as raw-material for paper pulp or as biofuel for combustion. A new harvest method, the delayed harvesting system, was developed which makes it possible to get dry, ready to store, material. The method reduces cost and improves quality of the product. The use of RCG as an industrial crop requires a completely different quality compared to forage. The important part is the cellulose while protein and mineral nutrients are disturbing the process …
Tall Tropical Grasses And Leucaena As Energy Crops For Lower South Usa,
2024
University of Florida
Tall Tropical Grasses And Leucaena As Energy Crops For Lower South Usa, G M. Prine, K R. Woodard
IGC Proceedings (1977-2023)
The tropical leguminous shrub/tree, leucaena (Leucaena spp. mainly leucocephala), and perennial tropical tall grasses such as elephantgrass (Pennisetum purpureum), sugarcane, and energycane (Saccharum spp.) are well adapted to the long growing seasons and high rainfall of humid lower South. In much of the area the topgrowth is killed by frost during winter and plants regenerate from underground parts in spring. Selected accessions from a duplicated 373 accession leucaena nursery had an average annual woody stem dry matter production of 31.4 Mg ha-1 and four seasons growth of 78.9 Mg ha-1. The tall perennial grasses have …
Energy Balance Of Different Intensive Forage Cropping Systems In Northern Italy,
2024
Instituto Sperimentale per la Colture Foraggere, Italy
Energy Balance Of Different Intensive Forage Cropping Systems In Northern Italy, M Onofrii, C Tomasoni, L Borrelli, L Bechini
IGC Proceedings (1977-2023)
Five forage crop rotations at two intensification levels have been compared using energy analysis, to have an integrated view on the systems under study. Inputs required by the five rotations ranged from 33 to 72 GJ•ha-1•yr1• Outputs from cropping systems ranged from41 to 153 GJ•ha-1•yr1 and output/input ratios from 1,25 to 2,13. The most efficient rotations in terms of net energy production efficiency have been characterised by reduced length and presence of maize and catch-crops. Low intensification level has caused higher efficiency in three rotations.
How Do Cattle And Sheep Alter Ingestive Behaviour In Response To Changes In Sward State?,
2024
Institute of Grassland and Environmental Research, UK
How Do Cattle And Sheep Alter Ingestive Behaviour In Response To Changes In Sward State?, P D. Penning, R J. Orr, A J. Parsons, A Harvey
IGC Proceedings (1977-2023)
Ingestive behaviour of yearling Friesian heifers continuously stocked on monocultures of Lolium perenne (G) or Trifolium repens (C) maintained at sward heights of 7-8 cm, was recorded. Bite masses, prehension biting and mastication rates were similar between treatments (211 vs. 230 mg DM prehension bite-1, 61 vs. 55 prehension bites and 11 vs. 13 mastications, min-1 for G and C, respectively). DM intake rates were 12.9 g min-1 for both treatments. Animals grazed longer (536 vs.436 min) and ruminated longer (526 vs. 267 min-1) on G compared with C. Daily intakes were 6.9 vs.5.6 kg DM for G and C. …
Effects Of Fasting On Ingestive Behaviour Of Sheep Grazing Grass Or White Clover Monocultures,
2024
Institute of Grassland and Environmental Research
Effects Of Fasting On Ingestive Behaviour Of Sheep Grazing Grass Or White Clover Monocultures, P D. Penning, M Boval
IGC Proceedings (1977-2023)
Effects of fasting sheep for 24 h (F), compared with controls (NF), on their ingestive behaviour and particle size in extrusa was investigated using oesophageally fistulated animals, grazing monocultures of Lolium perenne (G) or Trifolium repens (C). Bite masses tended to be lower on G than C and tended to increase with fasting (40, 64, 61 and 70 mg DM bite-1) for treatments GNF, GF, CNF and CF, respectively. Prehension rate was lower (52 vs. 69 bites min-1) and masticating rate was higher (106 vs.86 mastications min- 1) for G and C treatments, respectively. Over 24 h following fasting, NF …
Performance Of Dairy Cows, Feed Quality And N Balance On Pasture Systems With Or Without Mineral N Fertilizer,
2024
University of Bonn, Germany
Performance Of Dairy Cows, Feed Quality And N Balance On Pasture Systems With Or Without Mineral N Fertilizer, M Rodehutscord, Ina Hahner, H Spiekers, E Pfeffer
IGC Proceedings (1977-2023)
In a 2 year experiment, two levels of mineral N fertilization (nil and 225 kg N/ha) on pasture were checked for their effects on pasture yield and quality as well as for performance of dairy cows. Twenty six cows were used in each treatment and the stocking density was adapted to previous results on pasture growth rates. Cows were kept on pasture during summer and grass silages were harvested from the respective experimental areas for winter feeding periods. Digestibility of organic matter in these silages, determined in wether sheep, was always higher in those silages harvested from pasture without mineral …
Effect Of Milk Yield, Sward Height And Concentrate Supplementation Level On Herbage Intake And Grazing Behaviour Of Set-Stocked Dairy Cattle In Spring,
2024
Univeridad Austral de Chile
Effect Of Milk Yield, Sward Height And Concentrate Supplementation Level On Herbage Intake And Grazing Behaviour Of Set-Stocked Dairy Cattle In Spring, R Pulido, J D. Leaver
IGC Proceedings (1977-2023)
An experiment was carried out in spring over 42 days, with 48 multiparous Holstein Friesian cows, to examine the interrelationship of milk yield level, sward height and concentrate level. Two initial milk yield groups of 21.3 and 35.5 kg/day, two range of compressed sward heights of 3 to 5 cm, and 7 to 9 cm , and three concentrate levels of 0 and 6 kg/day and ad libitum were evaluated in a factorial design. Multiple regression analyses were carried out relating intake and behavioural dependent variables to the independent variables initial milk yield (IMY), sward height (SH) and concentrate intake …
Ruminal Fill Effect Of Forages: Prediction And Relationship With Voluntary Intake,
2024
Centre de Clermont-Fd/Theix, France
Ruminal Fill Effect Of Forages: Prediction And Relationship With Voluntary Intake, R Baumont, A Barlet, J Jamot
IGC Proceedings (1977-2023)
Voluntary dry matter intake (VDMI) and rumen fill were measured on sheep fed with 18 forages ranging from wheat straw to lucerne hay. In vivo fill effect (IVFE i.e. rumen DM pool divided by VDMI), in situ degradability, cell-wall composition, pepsin-cellulase digestibility and in vitro gas production were determined. In situ estimated fill effect (ISFE) was calculated as the retention time of insoluble potential degradable and undegradable fractions using a constant rate of passage. ISFE and IVFE were highly correlated (r2=0.89) but ISFE values were lower than IVFE values because in situ degradability does not integrate comminution time of …
Monitoring Of Intake And Energetic Efficiency Of The Grass In A Pasture Rotationally Grazed By Bulls Using Nirs Applied To The Faeces,
2024
CRAGx Station de Haute Belgique
Monitoring Of Intake And Energetic Efficiency Of The Grass In A Pasture Rotationally Grazed By Bulls Using Nirs Applied To The Faeces, Ph Lecomte, V Decruyenaere, P Limbourg, P Dardenne
IGC Proceedings (1977-2023)
The objective of this approach was to characterise variations that one can observe in the course of a pasture season using near infrared spectrometry (NIRS) applied to the analysis of the grass and faeces collected during the season. Available grass and faeces of Belgian Blue White young bulls have been collected 3 times a week since the beginning of May until mid-October. The samples have been predicted in NIRS technique according to a calibration previously developed on the faeces of sheep fed ad libitum in cage with green grass. The NIRS provides a useful tool to provide a relative description …
Modeling Intake Of Grazing Cows Fed Complementary Feeds,
2024
Department of Agricultural and Biological Engineering, Penn State
Modeling Intake Of Grazing Cows Fed Complementary Feeds, D R. Buckmaster, L A. Holden, L D. Muller, R H. Mohtar
IGC Proceedings (1977-2023)
A model suitable for predicting intake for grazing or non-grazing dairy cows is presented. The model integrates the potentially intake limiting factors of physical fill, physiological energy demand, wet mass, herbage availability, herbage cover, and grazing time. Integration of these factors with a simple set of linear ration balancing constraints yields a model suitable for predicting supplemental feed requirements as well as potential animal production from a grazed land. The model is semi-theoretical, being descriptive in structure, but containing empirical relationships.
Intake And Quality Of Panicum Maximum (Cv. Gatton) Receiving Different Levels Of Nitrogen During Autumn,
2024
University of Pretoria
Intake And Quality Of Panicum Maximum (Cv. Gatton) Receiving Different Levels Of Nitrogen During Autumn, W.A. Van Niekerk, N F.G. Rethman
IGC Proceedings (1977-2023)
The objective of this study was to investigate the influence of N fertilization on the quality of Panicum maximum cv. Gatton (Panicum) and the subsequent intake thereof. Hand cut samples increased in N (1.8 - 2.7%) and NO3-N content (0.75 - 1.54%) and decreased in WSC (14.6 - 13.9 g/kg) content, but with no changes in IVDOM as N fertilization levels raised from 0 to 150 kg N/ha respectively. No changes occurred in the NDF and ADL values. The animal selected N, and IVDOM values were higher and NDF value lower than the hand cut samples, which was expected due …
Intake And Partial Digestibility Of Panicum Maximum (Cv. Gatton) At Three Stages Of Maturity During Summer,
2024
University of Pretoria
Intake And Partial Digestibility Of Panicum Maximum (Cv. Gatton) At Three Stages Of Maturity During Summer, W.A. Van Niekerk, N F.G. Rethman
IGC Proceedings (1977-2023)
The objective of this study was to determine the influence of stage of maturity on the intake and digestibility of P. maximum (cv. Gatton) during the active growing season. Low DM content at the young and medium growth stages (24.9 and 25.2%) did not hamper DOMI (48.8 and 44.1 g/kg W0.75/d) respectively. The N concentration of the three stages were well within the limit required for optimal rumen microbial production (1.8 - 2.0%). The high NDF value of the adult stage (59.5%) resulted in a significantly lower IVDOM value (62.3%) but the IVDOM values of all stages were on the …
