Molecular Characterization And Expression Analysis Of Putative Rpk1 And Putative Clv1 In Malaysian Indica Rice (Oryza Sativa L.) Cv.Mr219 And Malaysian Wild Rice (Oryza Rufipogon) Irgc105491.,
2010
Universiti Malaya
Molecular Characterization And Expression Analysis Of Putative Rpk1 And Putative Clv1 In Malaysian Indica Rice (Oryza Sativa L.) Cv.Mr219 And Malaysian Wild Rice (Oryza Rufipogon) Irgc105491., Law Yee Song
Student Works (2010-2019)
Two candidate yield-related genes, putative Receptor-like Protein Kinase 1 (putative RPK1) and putative CLAVATA1 Receptor Kinase (putative CLV1), were selected for molecular characterization and expression analysis. This was carried out using in four rice lines comprising the parents and two BC2F7 lines from a local rice breeding program, with Malaysian rice variety, Oryza sativa ssp. indica cv. MR219 as recurrent parent, and Oryza rufipogon (IRGC105491) as donor parent. Approximately 2.8 kb and 2.6 kb putative RPK1 cDNA nucleotide sequences were obtained from Oryza rufipogon and Oryza sativa ssp. indica cv. MR219 respectively. However, only 402 …
Effects Of Plant Growth Regulators And Physiological Stresses On The Growth Of Bougainvillea Spp.,
2010
Universiti Malaya
Effects Of Plant Growth Regulators And Physiological Stresses On The Growth Of Bougainvillea Spp., Mohammed Saifuddin
Student Works (2010-2019)
This study was aimed to investigate the effects of physiological stresses such as pruning, shading, phloemic stress and plant hormones such as gibberellic acid (GA3) and naphthalene acetic acid (NAA) on the growth, development and flowering process of bougainvillea plants. The plants (Bougainvillea glabra and Bougainvillea spectabilis) were grown under prevailing conditions (temperature 21-32°C, maximum PAR 2100 μE m-2 s-1 and relative humidity of 60-90%) using completely randomized design. Field experiments as well as biochemical analysis were carried out at the Plant Physiology Garden and the Plant Physiology Laboratory, Institute of Biological Sciences, University of Malaya. Results on frequent pruning …
Characterization Of Microrna172 And Its Potential Target In Oil Palm (Elaeis Guineensis Jaq.),
2010
Universiti Malaya
Characterization Of Microrna172 And Its Potential Target In Oil Palm (Elaeis Guineensis Jaq.), Fatemeh Mehrpooyan
Student Works (2010-2019)
Oil palm (Elaeis guineensis) is the world’s major perennial monocot crop and contributes highly to the economies of South East Asia. As the oil palm fruit is the source of the main economic output for this crop, floral development is a critical process related to oil palm productivity. miR172 has been shown to be an important regulator of the AP2 family transcription factors involved in floral timing in a number of plants and belongs to a group of highly conserved miRNA that can be identified based on sequence homology. Six oil palm miR172 paralogs, representing two distinct mature miR172 sequences, …
Phylogeographical Patterns Of Neobalanocarpus Heimii (Dipterocarpaceae) For The Evolutionary History And Chain Of Custody Certification.,
2010
Universiti Malaya
Phylogeographical Patterns Of Neobalanocarpus Heimii (Dipterocarpaceae) For The Evolutionary History And Chain Of Custody Certification., Tnah Lee Hong
Student Works (2010-2019)
Tectonic movement and climatic oscillations during the Cenozoic have had dramatic effect on the biota of the tropical rain forest. This study aims to reveal the phylogeography and evolutionary history of a Peninsular Malaysian endemic tropical timber, Neobalanocarpus heimii (Dipterocarpaceae), based on chloroplast DNA (cpDNA) variation. Fifteen haplotypes were identified from 10 intraspecific variable sites of five non-coding cpDNA regions: trnL intron, trnS-trnG spacer, trnG intron, trnK intron and psbK-trnS spacer. Two major genealogical cpDNA lineages of N. heimii were elucidated: a widespread southern and a northern region. The species is predicted to survive in multiple refugia during climatic oscillation: …
Studies Of Antioxidant Activity Of Bioactive Compounds Derived From Ardisia Crispa.,
2010
Universiti Malaya
Studies Of Antioxidant Activity Of Bioactive Compounds Derived From Ardisia Crispa., Hassan Mahmood Kzar Jindal
Student Works (2010-2019)
In recent times, researches have focused on medicinal plants in order to extract natural and low cost antioxidants that can help protect the human body against oxidative stress, and replace the synthetic additives that might be carcinogenic. In present study, the crude extracts of leaves and fruits from Ardisia crispa were investigated for their chemical constituents and antioxidant activity. Preliminary phytochemical screening of the crude extracts via TLC analysis revealed the presence of several secondary compounds mainly, terpenoids and phenolic compounds in addition to alkaloids and saponins. HPLC analysis showed the possibility of presence of gallic acid in the fruits …
Studies On The Bioactive Properties Of Selected Plant And Fermented Extracts.,
2010
Universiti Malaya
Studies On The Bioactive Properties Of Selected Plant And Fermented Extracts., Saravana Kumar Sinniah
Student Works (2010-2019)
The aim of this study was to isolate protein/peptide and secondary metabolites from plant and fermented sources. The extracts were used to test for antimicrobial and antioxidant properties. In the current study protein/peptide extracts of Allium sativum showed promising results with a 15mm inhibition zone against Escherichia coli, 28mm against Staphylococcus aureus, 16.3mm against Bacillus cereus and 9mm against Pseudomonas aeruginosa. The minimum bacteriocidal concentration (MBC) results showed that Allium sativum protein/peptide extract is only bacteriostatic and not bacteriocidal. Andrographis paniculata showed the most promising result for the ethanolic extracts with an inhibition zone of 14mm against B. cereus, 11.5mm …
A Systematic Study Of Fagraea Sensu Lato And A Revision Of The Peninsular Malaysian Taxa.,
2010
Universiti Malaya
A Systematic Study Of Fagraea Sensu Lato And A Revision Of The Peninsular Malaysian Taxa., Sugumaran Manickam
Student Works (2010-2019)
systematic study of the Peninsular Malaysian species of Fagraea sensu lato was undertaken. This was done in the light of recent revisionary work done for Borneo that documented 20 new species and demonstrated that previous species concepts for Fagraea were too broad. Parallel to this the distinction of the subgeneric groups recognised as sections, i.e., Cyrtophyllum, Fagraea and Racemosae, were investigated with molecular phylogenetic methods. Representative taxa from the Malay Peninsula and Borneo augmented by sequences from other taxa in the same subtribe and tribe were used in the molecular analysis. Gene sequences from ITS, trnL–F (trnL intron + trnL–F …
Chemical Constituents Of Aglaia Lanuginose. King,
2010
Universiti Malaya
Chemical Constituents Of Aglaia Lanuginose. King, Fadzly Adzhar Kamarulzaman
Student Works (2010-2019)
Aglaia is a small genus of trees that belong to the family Meliaceae. They are widely distributed in India, Malay Peninsula and throughout Indomalesia. The chemistry of Aglaia has been extensively investigated and most of the researches done on these plants focused on their effect as insecticides, antifeedant, growth inhibitors, cytotoxicity and others. The dichloromethane extract from the bark of Aglaia lanuginose were studied. The chromatographic separation on both of the extracts gave ten compounds. The compounds isolated were identified as eichlerialactone 102, methyl eichlerianate 103, cabraleone 104, ocotillone 105, 4-hydroxycinnamyl acetate 106, cabralealactone 107, eichlerianic acid 108, shoreic acid …
Bioactivity And Chemical Investigations Of Pereskia Bleo And Pereskia Grandifollia.,
2010
Universiti Malaya
Bioactivity And Chemical Investigations Of Pereskia Bleo And Pereskia Grandifollia., Sim Kae Shin
Student Works (2010-2019)
Ethnopharmacological data has been one of the common useful criteria in drug discovery. Pereskia bleo and Pereskia grandifolia (Cactaceae), commonly known as ‘Jarum Tujuh Bilah’ in Malay and ‘Cak Sing Cam’ in Chinese have long been used as natural remedies in Malaysia. The experimental approach in the present study was based on bioassay-guided fractionation. The crude methanol and fractionated extracts of both Pereskia spp. were initially investigated for their biological activities such as antioxidant, antimicrobial and cytotoxic effect against five human cancer cell lines, namely nasopharyngeal epidermoid carcinoma cell line (KB), cervical carcinoma cell line (CasKi), colon carcinoma cell line …
Chemical Constituents Of Cryptocarya Densiflora.,
2010
Universiti Malaya
Chemical Constituents Of Cryptocarya Densiflora., Nurul Nazneem Wan Othman
Student Works (2010-2019)
The extraction of alkaloids from the Malaysian Cryptocarya densiflora has been carried out in this study. The alkaloids were extracted from the bark and leaves of this species using acid base extraction and the crude alkaloids obtained were subjected to extensive chromatographic techniques such as thin layer and column chromatography. The structural elucidation of the purified alkaloids were performed with the aid of spectroscopic methods i.e. 1H NMR, 13C NMR, 2D NMR, IR, UV and LCMS. Investigation of the alkaloidal content from the bark of Cryptocarya densiflora afforded six alkaloids and their structures were elucidated as laurotetanine 63, isocaryachine 64, …
Deep And Comparative Transcriptome Analysis
Of Rice Plants Infested By The Beet Armyworm (Spodoptera Exigua) And Water Weevil (Lissorhoptrus Oryzophilus),
2010
Ohio State University
Deep And Comparative Transcriptome Analysis Of Rice Plants Infested By The Beet Armyworm (Spodoptera Exigua) And Water Weevil (Lissorhoptrus Oryzophilus), R. C. Venu, M. Sheshu Madhav, M. V. Sreerekha, Kan Nobuta, Yuan Zhang, Peter Carswell, Michael J. Boehm, B. C. Meyers, Kenneth L. Korth, Guo-Liang Wang
Department of Plant Pathology: Faculty Publications
The beet armyworm (Spodoptera exigua) and the rice water weevil (Lissorhoptrus oryzophilus) are two important insect pests in rice production. To identify insect-responsive genes in rice, we performed a deep transcriptome analysis of Nipponbare rice leaves infested with both beet armyworm and water weevil using massively parallel signature sequencing (MPSS). Many antisense, alternative, and novel transcripts were commonly and specifically induced and suppressed in the infested tissue. Key genes involved in the defense metabolic pathways such as salicylic acid and jasmonic acid biosynthesis pathways were up-regulated in the infested leaves. To validate theMPSS results, we analyzed …
Selecting Strawberry Cultivars For Winter Greenhouse Production,
2010
University of Nebraska-Lincoln
Selecting Strawberry Cultivars For Winter Greenhouse Production, Ellen T. Paparozzi, Stacy A. Adams, George E. Meyer, M. Elizabeth Conley, Vicki L. Schlegel, Erin E. Blankenship, Paul E. Read
Department of Agronomy and Horticulture: Faculty Publications
Costs of transportation and food safety concerns have spawned an increase in public support of locally grown vegetables and fruit. With this in mind, a two-phase investigation was planned with the goal of combining low start up costs for sustainable greenhouse production with selection of strawberry cultivars that would provide the greatest number and largest size of berries. Additionally, berries from each cultivar will be analyzed for their beneficial nutraceutical properties to determine if there is a difference among cultivars.
Fine Mapping The Soybean Aphid Resistance Gene Rag1 In Soybean,
2010
University of Illinois
Fine Mapping The Soybean Aphid Resistance Gene Rag1 In Soybean, Ki-Seung Kim, Stephanie Bellendir, Karen A. Hudson, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers
Department of Agronomy and Horticulture: Faculty Publications
The soybean aphid (Aphis glycines Matsumura) is an important soybean [Glycine max (L.) Merr.] pest in North America. The dominant aphid resistance gene Rag1 was previously mapped from the cultivar ‘Dowling’ to a 12 cM marker interval on soybean chromosome 7 (formerly linkage group M). The development of additional genetic markers mapping closer to Rag1 was needed to accurately position the gene to improve the effectiveness of markerassisted selection (MAS) and to eventually clone it. The objectives of this study were to identify single nucleotide polymorphisms (SNPs) near Rag1 and to position these SNPs relative to Rag1. …
A High Density Integrated Genetic Linkage Map Of Soybean And The Development Of A 1536 Universal Soy Linkage Panel For Quantitative Trait Locus Mapping,
2010
USDA-ARS, Soybean Genomics and Improvement Laboratory, Beltsville, Maryland
A High Density Integrated Genetic Linkage Map Of Soybean And The Development Of A 1536 Universal Soy Linkage Panel For Quantitative Trait Locus Mapping, D. L. Hyten, Ik-Young Choi, Qijian Song, James E. Specht, Thomas E. Carter Jr., Randy C. Shoemaker, Eun-Young Hwang, Lakshmi K. Matukumalli, P. B. Cregan
Department of Agronomy and Horticulture: Faculty Publications
Single nucleotide polymorphisms (SNPs) are the marker of choice for many researchers due to their abundance and the high-throughput methods available for their multiplex analysis. Only recently have SNP markers been available to researchers in soybean [Glycine max (L.) Merr.] with the release of the third version of the consensus genetic linkage map that added 1141 SNP markers to the map. Our objectives were to add 2500 additional SNP markers to the soybean integrated map and select a set of 1536 SNPs to create a universal linkage panel for high-throughput soybean quantitative trait locus (QTL) mapping. The GoldenGate assay …
Inoculation With Arbuscular Mycorrhizal Fungi Or
Crop Rotation With Mycorrhizal Plants Improves
The Growth Of Maize In Limed Acid Sulfate Soil,
2010
Nihon University
Inoculation With Arbuscular Mycorrhizal Fungi Or Crop Rotation With Mycorrhizal Plants Improves The Growth Of Maize In Limed Acid Sulfate Soil, Hasao Higo, Katsunori Isobe, Dong-Jin Kang, Kazuhiro Ujie, Rhae A. Drijber, Ryuichi Ishii
Department of Agronomy and Horticulture: Faculty Publications
Arbuscular mycorrhizal fungi (AMF) improve the uptake of immobile mineral nutrients such as phosphate, thereby improving plant growth. In acid sulfate soil (ASS), AMF spore density is generally low which impacts root colonization and phosphate uptake. Thus, inoculation may help increase AMF colonization of crops grown in ASS. AMF spore density decreases after cultivation of a non-host crop or bare fallow. In addition, preceding crops affect the growth and yield of subsequent crops. The production of AMF inocula requires AMF-compatible plants. The objective of the present study is to elucidate the effect of preceding crops on the persistence of inoculated …
Abundance Of Ssr Motifs And Development Of Candidate Polymorphic Ssr Markers (Barcsoyssr_1.0) In Soybean,
2010
USDA-ARS, Soybean Genomics and Improvement Lab., Beltsville, MD
Abundance Of Ssr Motifs And Development Of Candidate Polymorphic Ssr Markers (Barcsoyssr_1.0) In Soybean, Qijian Song, Gaofeng Jia, Youlin Zhu, David Grant, Rex T. Nelson, Eun-Young Hwang, D. L. Hyten, P. B. Cregan
Department of Agronomy and Horticulture: Faculty Publications
Simple sequence repeat (SSR) genetic markers, also referred to as microsatellites, function in map-based cloning and for marker-assisted selection in plant breeding. The objectives of this study were to determine the abundance of SSRs in the soybean genome and to develop and test soybean SSR markers to create a database of locus-specific markers with a high likelihood of polymorphism. A total of 210,990 SSRs with di-, tri-, and tetranucleotide repeats of five or more were identified in the soybean whole genome sequence (WGS) which included 61,458 SSRs consisting of repeat units of di- (≥10), tri- (≥8), and tetranucleotide (≥7). Among …
Snail Grazing Facilitates Growth Of A Bloom-Forming Alga,
2010
Sacred Heart University
Snail Grazing Facilitates Growth Of A Bloom-Forming Alga, Michele Guidone, Carol S. Thornber, Emily Field
Biology Faculty Publications
Herbivory often has a negative effect on plants. However, there is a growing number of examples, primarily in terrestrial ecosystems, where herbivory causes an increase in plant size, growth rate, and/or reproductive output. In marine ecosystems, the positive effects of herbivores on macroalgae are not as well studied, although limited evidence exists for herbivore-induced increases in macroalgal growth rates via 2 mechanisms: nutrient addition via grazer defecation, and epiphyte removal. In this study, we examined the effects of grazing by the mud snail Ilyanassa obsoleta on Ulva lactuca, the dominant bloom-forming macroalga in many New England estuaries. We found …
An Integrative Approach To Genomic Introgression Mapping,
2010
Iowa State University
An Integrative Approach To Genomic Introgression Mapping, Andrew J. Severin, Gregory A. Peiffer, Wayne W. Xu, D. L. Hyten, Bruna Bucciarelli, Jamie A. O'Rourke, Yung-Tsi Bolon, David Grant, Andrew Farmer, Gregory D. May, Carroll P. Vance, Randy C. Shoemaker, Robert M. Stupar
Department of Agronomy and Horticulture: Faculty Publications
Near-isogenic lines (NILs) are valuable genetic resources for many crop species, including soybean (Glycine max). The development of new molecular platforms promises to accelerate the mapping of genetic introgressions in these materials. Here, we compare some existing and emerging methodologies for genetic introgression mapping: single-feature polymorphism analysis, Illumina GoldenGate single nucleotide polymorphism (SNP) genotyping, and de novo SNP discovery via RNA-Seq analysis of next-generation sequence data. We used these methods to map the introgressed regions in an iron-inefficient soybean NIL and found that the three mapping approaches are complementary when utilized in combination. The comparative RNA-Seq approach offers …
Fine Mapping Of The Soybean Aphid-Resistance Gene Rag2 In Soybean Pi 200538,
2010
University of Illinois
Fine Mapping Of The Soybean Aphid-Resistance Gene Rag2 In Soybean Pi 200538, Ki-Seung Kim, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers
Department of Agronomy and Horticulture: Faculty Publications
The discovery of biotype diversity of soybean aphid (SA: Aphis glycines Matsumura) in North America emphasizes the necessity to identify new aphid-resistance genes. The soybean [Glycine max (L.) Merr.] plant introduction (PI) 200538 is a promising source of SA resistance because it shows a high level of resistance to a SA biotype that can overcome the SA-resistance gene Rag1 from ‘Dowling’. The SA-resistance gene Rag2 was previously mapped from PI 200538 to a 10-cM marker interval on soybean chromosome 13 [formerly linkage group (LG) F]. The objective of this study was to fine map Rag2. This fine mapping …
Structural And Functional Divergence Of A 1-Mb Duplicated Region In The Soybean (Glycine Max) Genome And Comparison To An Orthologous Region From Phaseolus Vulgaris,
2010
Purdue University
Structural And Functional Divergence Of A 1-Mb Duplicated Region In The Soybean (Glycine Max) Genome And Comparison To An Orthologous Region From Phaseolus Vulgaris, Jer-Young Lin, Robert M. Stupar, Christian Hans, D. L. Hyten, Scott A. Jackson
Department of Agronomy and Horticulture: Faculty Publications
Soybean (Glycine max) has undergone at least two rounds of polyploidization, resulting in a paleopolyploid genome that is a mosaic of homoeologous regions. To determine the structural and functional impact of these duplications, we sequenced two ~1-Mb homoeologous regions of soybean, Gm8 and Gm15, derived from the most recent ~13 million year duplication event and the orthologous region from common bean (Phaseolus vulgaris), Pv5. We observed inversions leading to major structural variation and a bias between the two chromosome segments as Gm15 experienced more gene movement (gene retention rate of 81% in Gm15 versus 91% in …
