Other Plant Sciences Commons^™

Protocol For Enrichment Of The Membrane Proteome Of Mature Tomato Pollen, Puneet Paul, Palak Chaturvedi, Anida Mesihovic, Arindam Ghatak, Wolfram Weckwerth, Enrico Schleiff

Department of Agronomy and Horticulture: Faculty Publications

We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched fraction was then subjected to shotgun proteomics for identification of proteins, followed by in silico analysis to annotate and classify the detected proteins.

Nebline, June 2017

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: What's Biting You?

Food & Health

Farm & Acreage

Pests & Wildlife

Horticulture

Early Childhood

4-H & Youth

and other extension news and events

First Report Of Xanthomonas Vasicola Causing Bacterial Leaf Streak On Corn In The United States, K. A. Korus, J. M. Lang, A. O. Adesemoye, C. C. Block, N. Pal, J. E. Leach, T. A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

In 2014 and 2015, Zea mays samples (field, seed, and popcorn) were submitted to the Plant and Pest Diagnostic Clinic at the University of Nebraska–Lincoln displaying long, dark, yellow to brown, water-soaked, linear lesions confined to the interveinal spaces of the leaves. Initial symptoms appeared as small water-soaked flecks that expanded between veins to form irregular lesions. In some cases, the disease progressed to cover 40 to 50% of the leaf area. The disease was confirmed by symptom expression and bacterial streaming in 41 counties in Nebraska as well as Yuma Co., Colorado, and Phillips Co., Kansas, both contiguous to …

Cover Crops: A Primer, Joshua J. Miller, Katja Koehler-Cole, Rodrigo Werle, Daren Redfearn

Department of Plant Pathology: Faculty Publications

When deciding how best to use cover crops, it is important to consider the ultimate goal. Is it to increase soil organic matter, increase nutrient availability to subsequent crops, reduce soil compaction, supply forage for livestock, and/or suppress weeds? Answering these questions will help identify the cover crops that offer the best chance of success for meeting the goal. Primarily, cover crops are used to enhance soil conservation, nutrient cycling and supply, and weed control. However, these benefits vary based on the species of cover crop that is planted, so it is important to select the crop type that will …

Transcriptomic Resources For The Medicinal Legume Mucuna Pruriens: De Novo Transcriptome Assembly, Annotation, Identification And Validation Of Est-Ssr Markers, N. Sathyanarayana, Ranjith Kumar Pittala, Pankaj Kumar Tripathi, Ratan Chopra, Heikham Russiachand Singh, Vikas Belamkar, Pardeep Kumar Bhardwaj, Jeff J. Doyle, Ashley N. Egan

Department of Agronomy and Horticulture: Faculty Publications

Background:

The medicinal legume Mucuna pruriens (L.) DC. has attracted attention worldwide as a source of the anti-Parkinson’s drug L-Dopa. It is also a popular green manure cover crop that offers many agronomic benefits including high protein content, nitrogen fixation and soil nutrients. The plant currently lacks genomic resources and there is limited knowledge on gene expression, metabolic pathways, and genetics of secondary metabolite production. Here, we present transcriptomic resources for M. pruriens, including a de novo transcriptome assembly and annotation, as well as differential transcript expression analyses between root, leaf, and pod tissues. We also develop microsatellite markers …

A Comprehensive Analysis Of Alternative Splicing In Paleopolyploid Maize, Wenbin Mei, Sanzhen Liu, James C. Schnable, Cheng-Ting Yeh, Nathan M. Springer, Patrick S. Schnable, William Brad Barbazuk

Department of Agronomy and Horticulture: Faculty Publications

Identifying and characterizing alternative splicing (AS) enables our understanding of the biological role of transcript isoform diversity. This study describes the use of publicly available RNA-Seq data to identify and characterize the global diversity of AS isoforms in maize using the inbred lines B73 and Mo17, and a related species, sorghum. Identification and characterization of AS within maize tissues revealed that genes expressed in seed exhibit the largest differential AS relative to other tissues examined. Additionally, differences in AS between the two genotypes B73 and Mo17 are greatest within genes expressed in seed. We demonstrate that changes in the level …

Impact Of Burkholderia Phytofirmans Strain Psjn On The Ex Vitro Acclimatization And In Vitro Propagation Of Tissue Cultured Dionaea Muscipula (Venus Fly Trap), Peter L. Riley

Masters Theses, 2010-2019

Dionaea muscipula, Venus fly trap, is an endangered plant that is propagated primarily through tissue culture. Tissue cultured plants must be acclimatized to their new environments when removed from their test tubes and the ensuing stress can result in plant mortality. A plant growth promoting rhizobacterium, Burkholderia phytofirmans strain PsJN, has been utilized in biotic hardening of tissue cultured plants and in the amelioration of stresses, particularly drought stress in potted plants. We investigated whether this bacterium could improve either propagation or acclimatization of tissue cultured Venus fly traps. Standardized inoculations were performed by adjusting cell density of inoculant …

Phenotypic Variation And Genetic Purity Of The Original 'Prime-Jim®' X 'Arapaho' Population, Loren M. Luther

Horticulture Undergraduate Honors Theses

In 2013, Castro et al., produced the first linkage map for primocane-fruiting blackberries using the cross ‘Prime-Jim®’ x ‘Arapaho.’ This mapping population has been maintained since the original experiment with the hopes of conducting future studies. Further research was determined to be needed on the population to better characterize additional phenotypic traits, and since nine years had passed since the seedlings were established, there was a need to assess the continued genetic purity of the population using molecular methods. Phenotypic data was collected to analyze the variation of the population including soluble solids content, titratable acidity, average weight, shape, and …

Nebline, May 2017

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Biosolids Land Application Program Celebrating 25 Years of Benefiting the Community

Food & Health

Farm & Acreage

Pests & Wildlife

Horticulture

Early Childhood

4-H & Youth

and other extension news and events

Predicting Trait-Environment Relationships For Venation Networks Elong An Andes-Amazon Elevation Gradient, Benjamin Blonder, Norma Salinas, Lisa Patrick Bentley, Alexander Shenkin, Percy O. Chambi Porroa, Yolvi Valdez Tejeira, Cyrille Violle, Nikolaos M. Fyllas, Gregory R. Goldsmith, Roberta E. Martin, Gregory P. Asner, Sandra Diaz, Brian J. Enquist, Yadvinder Malhi

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Understanding functional trait-environment relationships (TERs) may improve predictions of community assembly. However, many empirical TERs have been weak or lacking conceptual foundation. TERs based on leaf venation networks may better link individuals and communities via hydraulic constraints. We report measurements of vein density, vein radius, and leaf thickness for more than 100 dominant species occurring in ten forest communities spanning a 3,300 m Andes-Amazon elevation gradient in Peru. We use these data to measure the strength of TERs at community scale and to determine whether observed TERs are similar to those predicted by physiological theory. We found strong support for …

Ultramafic Geoecology Of South And Southeast Asia, M.L. Galey, A. Van Der Ent, M.C.M. Iqbal, N. Rajakaruna

Biological Sciences

Globally, ultramafic outcrops are renowned for hosting foras with high levels of endemism, including plants with specialised adaptations such as nickel or manganese hyperaccumulation. Soils derived from ultramafic regoliths are generally nutrient-deficient, have major cation imbalances, and have concomitant high concentrations of potentially phytotoxic trace elements, especially nickel. The South and Southeast Asian region has the largest surface occurrences of ultramafic regoliths in the world, but the geoecology of these outcrops is still poorly studied despite severe conservation threats. Due to the paucity of systematic plant collections in many areas and the lack of georeferenced herbarium records and databased information, …

The Taxonomy, Reproduction, And Distribution Of Rare Plants: A Study Of Magnolia Sp. In The Río Zuñac Reserve, Ecuador, Alyssa Kullberg

Independent Study Project (ISP) Collection

Ecuadorian cloud forests are biodiversity hotspots and centers of unprecedented levels of endemism, but they are also under-researched and under-protected. This study took place in the Río Zuñac Reserve, which is a reserve of about 850 ha located in the Tungurahua province of Ecuador, and is home to at least 20 endemic plant species. Three new species of Magnolia have been discovered in this reserve in the past five years: Magnolia llanganates, Magnolia vargasiana, and one species that has yet to be formally described. This study dealt with accurately locating and describing new individuals of these species over the …

Nebline, April 2017

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Emerald Ash Borer — Next Steps

Food & Health

Farm & Acreage

Pests & Wildlife

Horticulture

Early Childhood

4-H & Youth

and other extension news and events

Special Pullout Section: Weed Awareness

Emergence, Competition, And Management Of Glyphosate-Resistant Common Ragweed (Ambrosia Artemisiifolia L.) In Nebraska Soybean, Ethann R. Barnes

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Common ragweed (Ambrosia artemisiifolia L.) is a competitive annual broadleaf weed in soybean (Glycine max) production fields throughout North America. The recent confirmation of glyphosate-resistant common ragweed in Nebraska justified the need to assess the emergence pattern and competitive ability of common ragweed in soybean and to evaluate alternative herbicide programs for effective management. The objectives of this research were to: 1) evaluate the effect of tillage and develop a predictive model for the emergence pattern of common ragweed in Nebraska; 2) model the competitive interaction between soybean and common ragweed as influenced by density and irrigation …

Surveying The Distributions Of Melaleuca Quinquenervia, Psidium Cattleianum, And Litsea Glutinosa At Analalava Special Reserve, Hanusia Higgins

Independent Study Project (ISP) Collection

This study surveyed the distributions of three invasive plant species, Melaleuca quinquenervia, Psidium cattleianum, and Litsea glutinosa at the Analalava Special Reserve in Mahavelona, Madagascar. Analalava is a 229-hectare degraded patch of low-elevation humid forest on the northeast coast of Madagascar, and one of the last forest fragments remaining in the region. It is a haven for biodiversity, containing 343 recorded species of plants, 12 of which are locally endemic. Invasive species are considered the second-most significant threat to biodiversity, both worldwide and specifically at Analalava. Based on a systematic transect survey of these three targeted invasive species, their distributions …

Investigation De Regeneración Natural De Plantas Vasculares En La Reserva Madrigal Del Podocarpus / Investigation Of Natural Regeneration Of Vascular Plants In The Madrigal Reserve Of The Podocarpus, Sy Baker

Independent Study Project (ISP) Collection

En el Sur de Ecuador, hay una historia larga de incendio que está asociada con la historia de la agricultura y ganadería a causa de las técnicas de tala y quema para despejar áreas para campos y también quemaduras intencionales para fertilizar los suelos y mejorar productividad agrícola (Fernandez et al 2015). En los últimos días de Noviembre de 2016, un incendio quemó campos, la Reserva Madrigal de Podocarpus, y parte del Parque Nacional Podocarpus en Loja, Ecuador—el enfoque de este estudio. En este estudio: (1) se registraron las especies y distribución de plantas pioneras y sobrevivientes, (2) la se …

Nebline, March 2017

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Extension’s Agricultural Apps Put Management Tools in Producers’ Hands

Food & Health

Farm & Acreage

Pests & Wildlife

Horticulture

Early Childhood

4-H & Youth

and other extension news and events

Novel Gene-Sequence Markers For Isolate Tracking Within Monilinia Fructicola Lesions, Madeline E. Dowling, Guido Schnabel, Harriet G. Boatwright, Sydney E. Everhart

Department of Plant Pathology: Faculty Publications

Background: Monilinia fructicola is a diverse pathogen of pome and stone fruits that causes severe economic losses each year. However, little is known about inoculum flow within or between orchards and pathogen establishment in an orchard, because few methods exist for detecting diversity or tracking isolates over time. SSR loci are an effective option, but may be confounded by a high degree of mutability and potential sensitivity to abiotic stress.

Results: Through transcriptome analysis, we identified novel markers mrr1, DHFR and MfCYP01 and validated stability of these markers under fungicide stress in natural infection sites. Nucleotide variation …

Nebline, February 2017

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 3rd Graders Learn About Life Cycle By Hatching Eggs in Classrooms

Food & Health

Farm & Acreage

Pests & Wildlife

Horticulture

Early Childhood

4-H & Youth

and other extension news and events

Corn Residue Use By Livestock In The United States, Marty R. Schmer, Rachael M. Brown, Virginia L. Jin, Robert B. Mitchell, Daren D. Redfearn

Department of Agronomy and Horticulture: Faculty Publications

Corn (Zea mays L.) residue grazing or harvest provides a simple and economical practice to integrate crops and livestock, but limited information is available on how widespread corn residue utilization is practiced by US producers. In 2010, the USDA Economic Research Service surveyed producers from 19 states on corn grain and residue management practices. Total corn residue grazed or harvested was 4.87 million ha. Approximately 4.06 million ha was grazed by 11.7 million livestock (primarily cattle) in 2010. The majority of grazed corn residue occurred in Nebraska (1.91 million ha), Iowa (385,000 ha), South Dakota (361,000 ha), and Kansas …