Principles, Applications, And Biosafety Of Plant Genome Editing Using Crispr-Cas9,
2020
Kuztown University of Pennsylvania
Principles, Applications, And Biosafety Of Plant Genome Editing Using Crispr-Cas9, Kaoutar El-Mounadi, María Luisa Morales-Floriano, Hernan Garcia-Ruiz
Department of Plant Pathology: Faculty Publications
The terms genome engineering, genome editing, and gene editing, refer to modifications (insertions, deletions, substitutions) in the genome of a living organism. The most widely used approach to genome editing nowadays is based on Clustered Regularly Interspaced Short Palindromic Repeats and associated protein 9 (CRISPR-Cas9). In prokaryotes, CRISPR-Cas9 is an adaptive immune system that naturally protects cells from DNA virus infections. CRISPR-Cas9 has been modified to create a versatile genome editing technology that has a wide diversity of applications in medicine, agriculture, and basic studies of gene functions. CRISPR-Cas9 has been used in a growing number of monocot and dicot …
Evaluating The Impact Of Grafting On Local Tomato Production In Nebraska,
2020
University of Nebraska-Lincoln
Evaluating The Impact Of Grafting On Local Tomato Production In Nebraska, Raihanah Hassim, Samuel E. Wortman, Ashley A. Thompson, Stacy A. Adams
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
Grafting has been successfully used in vegetable production for tomatoes, peppers, eggplants, cucumbers, and watermelon. Besides its usefulness for managing soil-borne diseases, grafting can improve nutrient uptake and yield. However, few studies have assessed the effects of grafting and soil fertility management on yield of open field-grown tomatoes in the Midwest. Therefore, the objective of this two-year research was to better document the effects of grafting heirloom and hybrid tomato cultivar onto hybrid tomato rootstocks on tomato yield and quality. The field experiments were located at the University of Nebraska Lincoln - East Campus in Lincoln, West Central Research and …
Mutations In Virus-Derived Small Rnas,
2020
University of Nebraska-Lincoln
Mutations In Virus-Derived Small Rnas, Deepti Nigam, Katherine Latourrette, Hernan Garcia Ruiz
Department of Plant Pathology: Faculty Publications
RNA viruses exist as populations of genome variants. Virus-infected plants accumulate 21–24 nucleotide small interfering RnAs (siRnAs) derived from viral RnA (virus-derived siRnAs) through gene silencing. This paper describes the profile of mutations in virus-derived siRNAs for three members of the family Potyviridae: Turnip mosaic virus (tuMV), Papaya ringspot virus (pRSV) and Wheat streak mosaic virus (WSMV). for tuMV in Arabidopsis thaliana, profiles were obtained for mechanically inoculated rosette leaves and systemically infected cauline leaves and inflorescence. Results are consistent withselection pressure on the viral genome imposed by local and systemic movement. By genetically removing gene silencing in the plant …
Variation Profile Of The Orthotospovirus Genome,
2020
University of Nebraska-Lincoln
Variation Profile Of The Orthotospovirus Genome, Deepti Nigam, Hernan Garcia Ruiz
Department of Plant Pathology: Faculty Publications
Orthotospoviruses are plant-infecting members of the family Tospoviridae (order Bunyavirales), have a broad host range and are vectored by polyphagous thrips in a circulative- propagative manner. Because diverse hosts and vectors impose heterogeneous selection constraints on viral genomes, the evolutionary arms races between hosts and their pathogens might be manifested as selection for rapid changes in key genes. These observations suggest that orthotospoviruses contain key genetic components that rapidly mutate to mediate host adaptation and vector transmission. Using complete genome sequences, we profiled genomic variation in orthotospoviruses. Results show that the three genomic segments contain hypervariable areas at homologous locations …
The Lateral Root Density Gene Regulates Root Growth During Water Stress In Wheat,
2020
University of Nebraska - Lincoln
The Lateral Root Density Gene Regulates Root Growth During Water Stress In Wheat, Dante F. Placido, Jaspreet Sandhu, Shirley Sato, Natalya Nersesian, Truyen Quach, Thomas E. Clemente, Paul E. Staswick, Harkamal Walia
Department of Agronomy and Horticulture: Faculty Publications
Drought stress is the major limiting factor in agriculture. Wheat, which is the most widely grown crop in the world, is predominantly cultivated in drought-prone rainfed environments. Since roots play a critical role in water uptake, root response to water limitations is an important component for enhancing wheat adaptation. In an effort to discover novel genetic sources for improving wheat adaptation, we characterized a wheat translocation line with a chromosomal segment from Agropyron elongatum, a wild relative of wheat, which unlike common wheat maintains root growth under limited-water conditions. By exploring the root transcriptome data, we found that reduced …
Rhizosphere Microbiome Of Arid Land Medicinal Plants And Extra Cellular Enzymes Contribute To Their Abundance,
2020
University of Nizwa
Rhizosphere Microbiome Of Arid Land Medicinal Plants And Extra Cellular Enzymes Contribute To Their Abundance, Abdul Latif Khan, Sajjad Asaf, Raeid M. M. Abed, Yen Ning Chai, Ahmed N. Al-Rawahi, Tupan Kumar Mohanta, Ahmed Al-Rawahi, Daniel P. Schachtman, Ahmed Al-Harrasi
Department of Agronomy and Horticulture: Faculty Publications
Revealing the unexplored rhizosphere microbiome of plants in arid environments can help in understanding their interactions between microbial communities and plants during harsh growth conditions. Here, we report the first investigation of rhizospheric fungal and bacterial communities of Adenium obesum, Aloe dhufarensis and Cleome austroarabica using next-generation sequencing approaches. A. obesum and A. dhufarensis grows in dry tropical and C. austroarabica in arid conditions of Arabian Peninsula. The results indicated the presence of 121 fungal and 3662 bacterial operational taxonomic units (OTUs) whilst microbial diversity was significantly high in the rhizosphere of A. obesum and A. dhufarensis and low …
Wheat Growth Monitoring And Yield Estimation Based On Multi-Rotor Unmanned Aerial Vehicle,
2020
Nanjing Agricultural University
Wheat Growth Monitoring And Yield Estimation Based On Multi-Rotor Unmanned Aerial Vehicle, Zhaopeng Fu, Jie Jang, Yang Gao, Brian Krienke, Meng Wang, Kaitai Zhong, Qiang Cao, Yongchao Tian, Yan Zhu, Weixing Cao, Xiaojun Liu
Department of Agronomy and Horticulture: Faculty Publications
Leaf area index (LAI) and leaf dry matter (LDM) are important indices of crop growth. Real-time, nondestructive monitoring of crop growth is instructive for the diagnosis of crop growth and prediction of grain yield. Unmanned aerial vehicle (UAV)-based remote sensing is widely used in precision agriculture due to its unique advantages in flexibility and resolution. This study was carried out on wheat trials treated with different nitrogen levels and seeding densities in three regions of Jiangsu Province in 2018–2019. Canopy spectral images were collected by the UAV equipped with a multi-spectral camera during key wheat growth stages. To verify the …
The State Of Sustainable Agriculture And Agroecology Research And Impacts: A Survey Of U.S. Scientists,
2020
Union of Concerned Scientists
The State Of Sustainable Agriculture And Agroecology Research And Impacts: A Survey Of U.S. Scientists, Marcia Delonge, Tali Robbins, Andrea D. Basche, Lindsey Haynes-Maslow
Department of Agronomy and Horticulture: Faculty Publications
A growing body of research suggests that although sustainable agriculture, particularly agroecology, can address challenges such as those related to climate change, ecosystem services, food insecurity, and farmer livelihoods, the transition to such systems remains limited. To gain insight into the state of U.S. sustainable agriculture and agroecology, we developed a 28-question mixed-method survey that was administered to scientists in these fields. Respondents (N=168) represented diverse locations, institutions, and career stages. They offered varied definitions of sustainable agriculture, with 40% considering economic and social well-being to be core components. Respondents identified the amount and duration of public research …
Multi-Trait Random Regression Models Increase Genomic Prediction Accuracy For A Temporal Physiological Trait Derived From High-Throughput Phenotyping,
2020
Virginia Polytechnic Institute and State University
Multi-Trait Random Regression Models Increase Genomic Prediction Accuracy For A Temporal Physiological Trait Derived From High-Throughput Phenotyping, Toshimi Baba, Mehdi Momen, Malachy T. Campbell, Harkamal Walia, Gota Morota
Department of Agronomy and Horticulture: Faculty Publications
Random regression models (RRM) are used extensively for genomic inference and predic- tion of time-valued traits in animal breeding, but only recently have been used in plant sys- tems. High-throughput phenotyping (HTP) platforms provide a powerful means to collect high-dimensional phenotypes throughout the growing season for large populations. How- ever, to date, selection of an appropriate statistical genomic framework to integrate multiple temporal traits for genomic prediction in plants remains unexplored. Here, we demonstrate the utility of a multi-trait RRM (MT-RRM) for genomic prediction of daily water usage (WU) in rice (Oryza sativa) through joint modeling with shoot …
Gwas: Fast-Forwarding Gene Identification And Characterization In Temperate Cereals: Lessons From Barley – A Review,
2020
Leibniz Institute of Plant Genetics and Crop Plant Research
Gwas: Fast-Forwarding Gene Identification And Characterization In Temperate Cereals: Lessons From Barley – A Review, Ahmad M. Alqudah, Ahmed Sallam, P. Stephen Baenziger, Andreas Börner
Department of Agronomy and Horticulture: Faculty Publications
Understanding the genetic complexity of traits is an important objective of small grain temperate cereals yield and adaptation improvements. Bi-parental quantitative trait loci (QTL) linkage mapping is a pow- erful method to identify genetic regions that co-segregate in the trait of interest within the research pop- ulation. However, recently, association or linkage disequilibrium (LD) mapping using a genome-wide association study (GWAS) became an approach for unraveling the molecular genetic basis underlying the natural phenotypic variation. Many causative allele(s)/loci have been identified using the power of this approach which had not been detected in QTL mapping populations. In barley (Hordeum …
Soil Carbon Increased By Twice The Amount Of Biochar Carbon Applied After 6 Years: Field Evidence Of Negative Priming,
2020
University of Nebraska-Lincoln
Soil Carbon Increased By Twice The Amount Of Biochar Carbon Applied After 6 Years: Field Evidence Of Negative Priming, Humberto Blanco-Canqui, David A. Laird, Emily A. Heaton, Samuel Rathke, Bharat Sharma Acharya
Department of Agronomy and Horticulture: Faculty Publications
Applying biochar to agricultural soils has been proposed as a means of sequester-ing carbon (C) while simultaneously enhancing soil health and agricultural sustain-ability. However, our understanding of the long-term effects of biochar and annual versus perennial cropping systems and their interactions on soil properties under field conditions is limited. We quantified changes in soil C concentration and stocks, and other soil properties 6 years after biochar applications to corn (Zea mays L.) and dedicated bioenergy crops on a Midwestern US soil. Treatments were as fol-lows: no-till continuous corn, Liberty switchgrass (Panicum virgatum L.), and low-diversity prairie grasses, 45% big bluestem …
Transforming Unmanned Aerial Vehicle (Uav) And Multispectral Sensor Into A Practical Decision Support System For Precision Nitrogen Management In Corn,
2020
University of Nebraska-Lincoln
Transforming Unmanned Aerial Vehicle (Uav) And Multispectral Sensor Into A Practical Decision Support System For Precision Nitrogen Management In Corn, Laura J. Thompson, Laila A. Puntel
Department of Agronomy and Horticulture: Faculty Publications
Determining the optimal nitrogen (N) rate in corn remains a critical issue, mainly due to unaccounted spatial (e.g., soil properties) and temporal (e.g., weather) variability. Unmanned aerial vehicles (UAVs) equipped with multispectral sensors may provide opportunities to improve N management by the timely informing of spatially variable, in-season N applications. Here, we developed a practical decision support system (DSS) to translate spatial field characteristics and normalized difference red edge (NDRE) values into an in-season N application recommendation. On-farm strip-trials were established at three sites over two years to compare farmer’s traditional N management to a split-application N management guided by …
Benchmarking Impact Of Nitrogen Inputs On Grain Yield And Environmental Performance Of Producer Fields In The Western Us Corn Belt,
2020
University of Nebraska-Lincoln
Benchmarking Impact Of Nitrogen Inputs On Grain Yield And Environmental Performance Of Producer Fields In The Western Us Corn Belt, Fatima A.M. Tenorio, Eileen L. Mclellan, Alison J. Eagle, Kenneth G. Cassman, Daryl Andersen, Marie Krausnick, Russell Oaklund, John Thorburn, Patricio Grassini
Department of Agronomy and Horticulture: Faculty Publications
Benchmarking crop yields against nitrogen (N) input levels can help provide opportunities to improve N ferti-lizer efficiency and reduce N losses on maize in the US Corn Belt by identifying fields most likely to benefit from improved N management practices. Here, we evaluated a large producer database that includes field-level data on yield and applied N inputs from 9280 irrigated and rainfed fields over a 7-year period (2009–2015) in Nebraska (USA). A spatial framework, based on technology extrapolation domains, was used to cluster each field into spatial units with similar climate and soil type that represent 1.3 million ha of …
Prediction Strategies For Leveraging Information Of Associated Traits Under Single- And Multi-Trait Approaches In Soybeans,
2020
University of Nebraska-Lincoln
Prediction Strategies For Leveraging Information Of Associated Traits Under Single- And Multi-Trait Approaches In Soybeans, Reyna Persa, Arthur Bernardeli, Diego Jarquin
Department of Agronomy and Horticulture: Faculty Publications
The availability of molecular markers has revolutionized conventional ways to improve genotypes in plant and animal breeding through genome-based predictions. Several models and methods have been developed to leverage the genomic information in the prediction context to allow more efficient ways to screen and select superior genotypes. In plant breeding, usually, grain yield (yield) is the main trait to drive the selection of superior genotypes; however, in many cases, the information of associated traits is also routinely collected and it can potentially be used to enhance the selection. In this research, we considered different prediction strategies to leverage the information …
Overexpression Of Ferulate 5‑Hydroxylase Increases Syringyl Units In Sorghum Bicolor,
2020
USDA, Agricultural Research Service
Overexpression Of Ferulate 5‑Hydroxylase Increases Syringyl Units In Sorghum Bicolor, Hannah M. Tetreault, Tammy Gries, Nathan Palmer, Deanna L. Funnell-Harris, Shirley Sato, Zhengxiang Ge, Gautam Sarath, Scott Sattler
Department of Agronomy and Horticulture: Faculty Publications
Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of lignin monomer, is derived from polymerization of coniferyl alcohol. In this study, the effects of manipulating S-lignin biosynthesis in sorghum (Sorghum bicolor) were evaluated. Overexpression of sorghum F5H (SbF5H), under the control of the CaMV 35S promoter, increased both S-lignin levels and the ratio of S/G lignin, while plant growth and development …
Biological And Cellular Functions Of The Microdomain-Associated Fwl/Cnr Protein Family In Plants,
2020
University of Nebraska-Lincoln
Biological And Cellular Functions Of The Microdomain-Associated Fwl/Cnr Protein Family In Plants, Sandra Thibivilliers, Andrew Farmer, Marc Libault
Department of Agronomy and Horticulture: Faculty Publications
Membrane microdomains/nanodomains are sub-compartments of the plasma membrane enriched in sphingolipids and characterized by their unique protein composition. They play important roles in regulating plant development and plant-microbe interactions including mutualistic symbiotic interactions. Several protein families are associated with the microdomain fraction of biological membranes such as flotillins, prohibitins, and remorins. More recently, GmFWL1, a FWL/CNR protein exclusively expressed in the soybean nodule, was functionally characterized as a new microdomain-associated protein. Interestingly, GmFWL1 is homologous to the tomato FW2-2 protein, a major regulator of tomato fruit development. In this review, we summarize the knowledge gained about the biological, cellular, and …
Investigation Of Heat-Induced Changes In The Grain Yield And Grains Metabolites, With Molecular Insights On The Candidate Genes In Barley,
2020
Assiut University
Investigation Of Heat-Induced Changes In The Grain Yield And Grains Metabolites, With Molecular Insights On The Candidate Genes In Barley, Mona F.A. Dawood, Yasser S. Moursi, Ahmed Amro, P. Stephen Baenziger, Ahmed Sallam
Department of Agronomy and Horticulture: Faculty Publications
Heat stress is one of the abiotic stresses that cause a significant reduction in barley yield. Climate change will increase the number of heatwaves, which will result in more deterioration in the agricultural sector. Therefore, understanding the physiological changes that occur in the plant to tolerate heat stress is very important. A collection of 60 Egyptian spring barley genotypes has been tested for heat stress under field conditions. To quantify the changes in yield-related traits and the grain-reserve parameters as indicators for heat tolerance, several traits were scored. The causative genes that regulate the variation of all traits of interest …
Soil Sample Timing, Nitrogen Fertilization, And Incubation Length Influence Anaerobic Potentially Mineralizable Nitrogen,
2020
South Dakota State University
Soil Sample Timing, Nitrogen Fertilization, And Incubation Length Influence Anaerobic Potentially Mineralizable Nitrogen, Jason Clark, Kristen S. Veum, Fabián G. Fernández, Newell R. Kitchen, James J. Camberato, Paul Carter, Richard Ferguson, David W. Franzen, Daniel E. Kaiser, Carrie A. M. Laboski, Emerson Nafziger, Carl J. Rosen, John E. Sawyer, John F. Shanahan
Department of Agronomy and Horticulture: Faculty Publications
Understanding the variables that affect the anaerobic potentially mineralizable N (PMNan) test should lead to a standard procedure of sample collection and incubation length, improving PMNan as a tool in corn (Zea mays L.) N management. We evaluated the effect of soil sample timing (preplant and V5 corn development stage [V5]), N fertilization (0 and 180 kg ha−1) and incubation length (7, 14, and 28 d) on PMNan (0–30 cm) across a range of soil properties and weather conditions. Soil sample timing, N fertilization, and incubation length affected PMNan differently based on soil and weather conditions. Preplant vs. V5 PMNan …
Weather And Soil In The Us Midwest Influence The Effectiveness Of Single- And Split-Nitrogen Applications In Corn Production,
2020
South Dakota State University
Weather And Soil In The Us Midwest Influence The Effectiveness Of Single- And Split-Nitrogen Applications In Corn Production, Jason D. Clark, Fabián G. Fernández, James J. Camberato, Paul R. Carter, Richard B. Ferguson, David W. Franzen, Newell R. Kitchen, Carrie A. M. Laboski, Emerson Nafziger, John E. Sawyer, John F. Shanahan
Department of Agronomy and Horticulture: Faculty Publications
Splitting the N application into two or more timings may improve corn (Zea mays L.) grain yield and N recovery relative to a single-N application. A 49 site-year study across eight U.S. Midwestern states compared the effect of an at-planting (single-N application) and two split-N applications [45 (45+SD) or 90 kg N ha−1 (90+SD) at planting with the remainder of the total rate (180 or 270 kg N ha−1) applied at V9]. For split-N applications, soil and plant responses were similar between 45+SD and 90+SD 93–98% of the time, indicating the at-planting N rate of 45 kg N ha−1 may …
Organic Fertilizer Abrasive Grits Increase Soil Available Nitrogen, Plant Height, And Biomass,
2020
University of Nebraska - Lincoln
Organic Fertilizer Abrasive Grits Increase Soil Available Nitrogen, Plant Height, And Biomass, Michael Carlson, Frank Forcella, Samuel E. Wortman, David Clay, Sharon A. Clay
Department of Agronomy and Horticulture: Faculty Publications
In organic cropping systems, air-propelled abrasive grits can be used to control in-row weeds. If the applied abrasive grit is an approved organic fertilizer, these applications may serve a dual purpose of weed control and crop fertility. Laboratory soil incubations examined the N mineralization rates of several grit types with differing C/N ratios (Agra Grit [crushed walnut shells, 170:1], corncob grit [91:1], Sustane [composted turkey litter, 5.0:1], Phytaboost Plant Food [crushed and pelletized soybean meal, 5.0:1]). A greenhouse study determined plant wheat (Triticum aestivum L.), kale (Brassica napus pabluaria DC), and velvetleaf (Abu- tilon theophrasti Medik.) growth response in soils …
