Other Plant Sciences Commons^™

Editorial: New Insights Into Mechanisms Of Epigenetic Modifiers In Plant Growth And Development, Volume Ii, Ming Luo, Tomasz Jacek Sarnowski, Marc Libault, Gabino Ríos, Jean-Benoit Charron, Nitin Mantri, Shoudong Zhang

Department of Agronomy and Horticulture: Faculty Publications

As we have learned, chromatin modifications, including histone modifications and DNA methylation, play a key role in plant development (Ng and Bird, 1999). However, accumulated evidence shows that, besides chromatin biochemical modifications, other epigenetic regulations such as chromatin architecture also function at a pivot point to regulate plant development (Zhang et al., 2021). In this Research Topic, five research papers describe multiple developmental facets mediated by different epigenetic mechanisms besides histone modifications. These manuscripts report experimental evidence or summarize recent advances in epigenetic regulations of some important developmental genes or development-related mechanisms. This Research Topic allows readers to learn of …

A Complete Telomere-To-Telomere Assembly Of The Maize Genome, Jian Chen, Zijian Wang, Kaiwen Tan, Wei Huang, Junpeng Shi, Tong Li, Jiang Hu, Kai Wang, Chao Wang, Beibei Xin, Haiming Zhao, Weibin Song, Matthew B. Hufford, James C. Schnable, Weiwei Jin, China Agricultural University, Hainan Yazhou Bay Seed Laboratory

Department of Agronomy and Horticulture: Faculty Publications

A complete telomere-to-telomere (T2T) finished genome has been the long pursuit of genomic research. Through generating deep coverage ultralong Oxford Nanopore Technology (ONT) and PacBio HiFi reads, we report here a complete genome assembly of maize with each chromosome entirely traversed in a single contig. The 2,178.6 Mb T2T Mo17 genome with a base accuracy of over 99.99% unveiled the structural features of all repetitive regions of the genome. There were several super-long simple-sequence-repeat arrays having consecutive thymine–adenine– guanine (TAG) tri-nucleotide repeats up to 235 kb. The assembly of the entire nucleolar organizer region of the 26.8 Mb array with …

Genome-Wide Association Analysis For Emergence Of Deeply Sown Rice (Oryza Sativa) Reveals Novel Aus-Specific Phytohormone Candidate Genes For Adaptation To Dry-Direct Seeding In The Field, Sandeep A. Sakhale, Shailesh Yadav, Lindsay V. Clark, Alexander E. Lipka, Arvind Kumar, Erik J. Sacks

Department of Agronomy and Horticulture: Faculty Publications

Dry direct-seeded rice (dry-DSR) is typically sown deeply to circumvent the need for irrigation, and thus seedling emergence is a crucial trait affecting plant stand and yield. To breed elite cultivars that use less water and are climate-resilient, an understanding of the genomic regions and underlying genes that confer emergence in deeply sown dry-DSR would be highly advantageous. A combined diversity panel of 470 rice accessions (RDP1 plus aus subset of 3K RGP) was evaluated with 2.9 million single nucleotide polymorphisms (SNPs) to identify associations with dry-DSR traits in the field and component traits in a controlled-environment experiment. Using genome-wide …

Updating The Dual C And O Isotope—Gas-Exchange Model: A Concept To Understand Plant Responses To The Environment And Its Implications For Tree Rings, Rolf T. W. Siegwolf, Marco M. Lehmann, Gregory R. Goldsmith, Olga V. Churakova (Sidorova), Cathleen Mirande-Ney, Galina Timoveeva, Rosmarie B. Weigt, Matthias Saurer

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The combined study of carbon (C) and oxygen (O) isotopes in plant organic matter has emerged as a powerful tool for understanding plant functional responses to environmental change. The approach relies on established relationships between leaf gas exchange and isotopic fractionation to derive a series of model scenarios that can be used to infer changes in photosynthetic assimilation and stomatal conductance driven by changes in environmental parameters (CO₂, water availability, air humidity, temperature, nutrients). We review the mechanistic basis for a conceptual model, in light of recently published research, and discuss where isotopic observations do not match our …

Do Cover Crop Mixtures Improve Soil Physical Health More Than Monocultures?, Humberto Blanco-Canqui

Department of Agronomy and Horticulture: Faculty Publications

Rationale and Purpose — Adding multispecies cover crop (CC) mixtures could diversify the current simplified crop rotations and enhance soil health more than monoculture CCs. Further, CC mixtures with diverse plant species could adapt better to changing climatic and environmental conditions than monoculture CCs. However, our current understanding of the soil benefits of CC mixtures is still limited. This review discussed whether CC mixtures are better than monoculture CCs to improve soil physical health.

Methods — All studies published up to May 25, 2023, comparing soil physical properties between CC mixtures and their constituents grown as monocultures were searched in …

Exogenous Application Of Growth Regulators Improves The Growth And Quality Of Dahlia Variabilis L., Shahbaz Khan, Naeem Abbas, Zuhair Hasnain, Sami Ullah, Imran Hussain, Afroz Rais, Muhammad Atif Ghafoor, Danish Ibrar, Sohail Irshad, Maham Saddique, Muhammad Amjad Bashir, Samra Naseem, Muhammad Musawir Khan, Jawaher Alkahtani

The Philippine Agricultural Scientist

Dahlia (Dahlia variabilis L.), a bulb crop sold as cut flower, garden ornamental/potted plant, becomes a popular cut flower in the world. This experiment was designed to study the effects of foliar applied gibberellic acid, zinc, ascorbic acid, and thiamine on the growth and flower attributes of dahlia. The study was conducted under greenhouse conditions at the Floriculture Research Area, University of Agriculture, Faisalabad, Pakistan. There were thirteen treatment with three levels each; gibberellic acid, zinc sulphate, ascorbic acid, and thiamine. First, foliar application was done at vegetative stage (after 45 d of transplantation) and second, at the flowering …

Characterization Of Boreal-Arctic Vegetation Growth Phases And Active Soil Layer Dynamics In The High-Latitudes Of North America: A Study Combining Multi-Year In Situ And Satellite-Based Observations, Michael G. Brown

Dissertations, Theses, and Capstone Projects

This dissertation examined the seasonal freeze/thaw activity in boreal-Arctic soils and vegetation physiology in Alaska, USA and Alberta, Canada, using in situ environmental measurements and passive microwave satellite observations. The boreal-Arctic high-latitudes have been experiencing ecosystem changes more rapidly in comparison to the rest of Earth due to the presently warming climatic conditions having a magnified effect over Polar Regions. Currently, the boreal-Arctic is a carbon sink; however, recent studies indicate a shift over the next century to become a carbon source. High-latitude vegetation and cold soil dynamics are influenced by climatic shifts and are largely responsible for the regions …

Impact Of Nitrogen Availability On The Accumulation Of Vegetative Lipids In Sorghum, Leticia Felicio Pasqualino

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Sorghum (Sorghum bicolor (L.) Moench) is a versatile and prosperous feedstock crop for renewable energy production. With the increase in bioenergy demand, the production of higher oil content per biomass in a feedstock crop is a highly desirable trait for the biofuel conversion process yield. Especially if this outcome can be achieved using fewer inputs in the field, such as nitrogen. In microalgae species, nitrogen limitation has been associated with changes in the carbon storage metabolic pathway favoring triacylglycerol (TAG) accumulation. In this context, this study aimed to assess whether nitrogen starvation would result in a similar outcome in …

A Foundational Population Genetics Investigation Of The Sexual Systems Of Solanum (Solanaceae) In The Australian Monsoon Tropics Suggests Dioecious Taxa May Benefit From Increased Genetic Admixture Via Obligate Outcrossing, Jason T. Cantley, Ingrid E. Jordon-Thaden, Morgan Roche, Daniel S. Hayes, Stephamie Kate, Christopher T. Martine

Faculty Journal Articles

Solanum section Leptostemonum is an ideal lineage to test the theoretical framework regarding proposed evolutionary benefits of outcrossing sexual systems in comparison to cosexuality. Theoretically, non-cosexual taxa should support more genetic diversity within populations, experience less inbreeding, and have less genetic structure due to a restricted ability to self-fertilize. However, many confounding factors present challenges for a confident inference that inherent differences in sexual systems influence observed genetic patterns among populations. This study provides a foundational baseline of the population genetics of several species of different sexual systems with the aim of generating hypotheses of any factor—including sexual system—that influences …

2018–2019 Field Seasons Of The Maize Genomes To Fields (G2f) G X E Project, Dayane Dayane Lima, Alejandro Castro Aviles, Ryan Timothy Alpers, Bridget A. Mcfarland, Shawn Kaeppler, David Ertl, Maria Cinta Romay, Joseph L. Gage, James Holland, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint‑Garcia, Candice N. Hirsch, Elizabeth Hood, David C. Hooker, Joseph E. Knoll, Judith M. Kolkman, Sanzhen Liu, John Mckay, Richard Minyo, Danilo E. Moreta, Seth C. Murray, Rebecca Nelson, James C. Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Peter Thomison, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Jacob D. Washburn, Teclemariam Weldekidan, Randall J. Wisser, Wenwei Xu31, Natalia De Leon

Department of Agronomy and Horticulture: Faculty Publications

Objectives This report provides information about the public release of the 2018–2019 Maize G X E project of the Genomes to Fields (G2F) Initiative datasets. G2F is an umbrella initiative that evaluates maize hybrids and inbred lines across multiple environments and makes available phenotypic, genotypic, environmental, and metadata information. The initiative understands the necessity to characterize and deploy public sources of genetic diversity to face the challenges for more sustainable agriculture in the context of variable environmental conditions.

Data description Datasets include phenotypic, climatic, and soil measurements, metadata information, and inbred genotypic information for each combination of location and year. …

Induction And Suppression Of Gene Silencing In Plants By Nonviral Microbes, Eric Parperides, Kaoutar El Mounadi, Hernan Garcia Ruiz

Department of Plant Pathology: Faculty Publications

Gene silencing is a conserved mechanism in eukaryotes that dynamically regulates gene expression. In plants, gene silencing is critical for development and for maintenance of genome integrity. Additionally, it is a critical component of antiviral defence in plants, nematodes, insects, and fungi. To overcome gene silencing, viruses encode effectors that suppress gene silencing. A growing body of evidence shows that gene silencing and suppression of silencing are also used by plants during their interaction with nonviral pathogens such as fungi, oomycetes, and bacteria. Plant–pathogen interactions involve trans-kingdom movement of small RNAs into the pathogens to alter the function of genes …

Induction And Suppression Of Gene Silencing In Plants By Nonviral Microbes, Eric Parperides, Kaoutar El Mounadi, Hernan Garcia Ruiz

Department of Plant Pathology: Faculty Publications

Gene silencing is a conserved mechanism in eukaryotes that dynamically regulates gene expression. In plants, gene silencing is critical for development and for maintenance of genome integrity. Additionally, it is a critical component of antiviral defence in plants, nematodes, insects, and fungi. To overcome gene silencing, viruses encode effectors that suppress gene silencing. A growing body of evidence shows that gene silencing and suppression of silencing are also used by plants during their interaction with nonviral pathogens such as fungi, oomycetes, and bacteria. Plant–pathogen interactions involve trans-kingdom movement of small RNAs into the pathogens to alter the function of genes …

Creeper Legume, In Conjunction With Biochar, Is A Potential Tool To Minimize Soil Erosion, Sujani De Silva, Priyantha Indralal Yapa, Kushani Mahatantila, Saurav Das, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

Accelerated soil erosion and landslides are destructive consequences of road development and intensive agriculture in the central highlands of Sri Lanka. Properly designed vegetation covers can play a vital role in erosion control. Identifying a plant that can adapt to eroded land with a low-nutrient supply is critical for natural erosion management. A perennial creeper legume, Vigna marina, adaptable for marginal lands and used to control soil erosion in Australia, was introduced to Sri Lanka via the 2004 tsunami. The objective of this study was to assess V. marina under five different soil substrates, including a reference treatment (RT) …

Desalination: Adapting To A Changing Climate And An Increasing Demand For Freshwater, Rebecca A. Acosta

Master's Projects and Capstones

The state of California is just one place in the world that is experiencing an increasing demand for freshwater while also experiencing increasingly hotter conditions and longer periods of drought. There are a number of plants slated for development in the state of California but have been met with resistance from the public with concerns regarding their impacts to the surrounding marine environments. This paper provides background on desalination plants, provides evidence for a potential indicator species, identifies potential impacts to marine environments, and addresses stakeholder concerns and perceptions around desalination plants.

Effectiveness Of Tadreb-B1 And 1-Feh W3 Kasp Markers In Spring And Winter Wheat Populations For Marker-Assisted Selection To Improve Drought Tolerance, Shamseldeen Eltaher, Mostafa Hashem, Asmaa A. M. Ahmed, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam

Department of Agronomy and Horticulture: Faculty Publications

Due to the advances in DNA markers, kompetitive allele-specific PCR (KASP) markers could accelerate breeding programs and genetically improve drought tolerance. Two previously reported KASP markers, TaDreb-B1 and 1-FEH w3, were investigated in this study for the markerassisted selection (MAS) of drought tolerance. Two highly diverse spring and winter wheat populations were genotyped using these two KASP markers. The same populations were evaluated for drought tolerance at seedling (drought stress) and reproductive (normal and drought stress) growth stages. The single-marker analysis revealed a high significant association between the target allele of 1-FEH w3 and drought susceptibility in the spring …

Long-Term Conservation And Conventional Tillage Systems Impact Physical And Biochemical Soil Health Indicators In A Corn–Soybean Rotation, Anuoluwa O. Sangotayo, Jemila Chellappa, Udayakumar Sekaran, Sangeeta Bansal, Padma Angmo, Paul J. Jasa, Sandeep Kumar, Javed Iqbal

Department of Agronomy and Horticulture: Faculty Publications

Agricultural management practices tend to influence soil structure stabilization, mediating many physical, chemical, and biological processes in soils. Therefore, understanding the long-term effects of management practices on various soil health indicators is crucial to develop sustainable agricultural practices. This study aimed to assess the long-term conventional and conservation tillage effects on soil physical (aggregates) and biochemical (soil organic carbon [SOC], enzymes, and microbial biomass) parameters under a range of tillage practices in a corn–soybean rotation in Nebraska. The experiment was conducted at two locations as follows: (1) Concord site (36 years) with the three treatments: no-till (NT), disk, and moldboard …

Hybrid Allele‑Specific Chip‑Seq Analysis Identifies Variation In Brassinosteroid‑Responsive Transcription Factor Binding Linked To Traits In Maize, Thomas Hartwig, Michael Banf, Gisele Passaia Prietsch, Jia‑Ying Zhu, Isabel Mora‑Ramírez, Jos H. M. Schippers, Samantha J. Snodgrass, Arun S. Seetharam, Bruno Huettel, Judith M. Kolkman, Jiashi Yang, Julia Engelhorn, Zhi‑Yong Wang

Department of Agronomy and Horticulture: Faculty Publications

Background: Genetic variation in regulatory sequences that alter transcription factor (TF) binding is a major cause of phenotypic diversity. Brassinosteroid is a growth hormone that has major effects on plant phenotypes. Genetic variation in brassinosteroidresponsive cis-elements likely contributes to trait variation. Pinpointing such regulatory variations and quantitative genomic analysis of the variation in TF-target binding, however, remains challenging. How variation in transcriptional targets of signaling pathways such as the brassinosteroid pathway contributes to phenotypic variation is an important question to be investigated with innovative approaches.

Results: Here, we use a hybrid allele-specific chromatin binding sequencing (HASChseq) approach and …

Establishing Big Sagebrush Seedlings On The Colorado Plateau, Kari E. Veblen, Eric Thacker, Mark Larese-Casanova, Kyle Nehring, Mike Duniway, Colby Brungard

All Current Publications

Factors such as soil type and precipitation vary across rangeland landscapes, and these factors affect restoration outcomes and ultimately mean that “one size fits all” management strategies are not effective across large, complex landscapes. Big sagebrush (Artemisia tridentata) is a foundational rangeland species that is important to wildlife habitat across the western U.S. On the Colorado Plateau, sagebrush is important browse for ungulates, such as mule deer and pronghorn, which motivates a great deal of restoration effort. However, most scientific knowledge of big sagebrush comes from the Great Basin, and we know much less about how to restore sagebrush on …

Evaluating Planting Green And Herbicides For Integrated Weed Management And Their Effect On Soil Properties In Corn And Soybean In Nebraska, Trey Stephens

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Producers across the Midwest are finding new ways to implement cover crops into cropping systems and the practice of “Planting Green” is one of the newest uses of cover crops. When planting green, producers plant their row crops into actively growing cover crops and terminate the cover crop at time of planting or shortly after planting. This practice would allow for higher biomass accumulation of the cover crop and could aid in weed management of herbicide-resistant weeds. The objective of the first two studies was to evaluate planting green and its effect on soil-applied residual herbicides, weed management, dicamba/glyphosate-resistant soybean …

A Programmatic Geographic Information Systems Analysis Of Plant Hardiness Zones, Andrew Bowen

Electronic Theses and Dissertations

The Plant Hardiness Zone Map consists of thirteen geographical zones that describe whether a plant can survive based on average annual minimal temperatures. As climate change progresses, minimum temperatures in all regions are expected to change. This work programmatically evaluates predicted future climate projection data and converts it to United States Department of Agriculture-defined hardiness zones. Through the next 80 years, hardiness zones are projected to move poleward; in effect, colder zones will lose area and warmer zones will gain area globally. Some implications include changes in crop growing degree days, which could alter crop productivity, migration and settlement of …