Horticulture Commons^™

2018–2019 Field Seasons Of The Maize Genomes To Fields (G2f) G X E Project, Dayane Dayane Lima, Alejandro Castro Aviles, Ryan Timothy Alpers, Bridget A. Mcfarland, Shawn Kaeppler, David Ertl, Maria Cinta Romay, Joseph L. Gage, James Holland, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint‑Garcia, Candice N. Hirsch, Elizabeth Hood, David C. Hooker, Joseph E. Knoll, Judith M. Kolkman, Sanzhen Liu, John Mckay, Richard Minyo, Danilo E. Moreta, Seth C. Murray, Rebecca Nelson, James C. Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Peter Thomison, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Jacob D. Washburn, Teclemariam Weldekidan, Randall J. Wisser, Wenwei Xu31, Natalia De Leon

Department of Agronomy and Horticulture: Faculty Publications

Objectives This report provides information about the public release of the 2018–2019 Maize G X E project of the Genomes to Fields (G2F) Initiative datasets. G2F is an umbrella initiative that evaluates maize hybrids and inbred lines across multiple environments and makes available phenotypic, genotypic, environmental, and metadata information. The initiative understands the necessity to characterize and deploy public sources of genetic diversity to face the challenges for more sustainable agriculture in the context of variable environmental conditions.

Data description Datasets include phenotypic, climatic, and soil measurements, metadata information, and inbred genotypic information for each combination of location and year. …

Creeper Legume, In Conjunction With Biochar, Is A Potential Tool To Minimize Soil Erosion, Sujani De Silva, Priyantha Indralal Yapa, Kushani Mahatantila, Saurav Das, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

Accelerated soil erosion and landslides are destructive consequences of road development and intensive agriculture in the central highlands of Sri Lanka. Properly designed vegetation covers can play a vital role in erosion control. Identifying a plant that can adapt to eroded land with a low-nutrient supply is critical for natural erosion management. A perennial creeper legume, Vigna marina, adaptable for marginal lands and used to control soil erosion in Australia, was introduced to Sri Lanka via the 2004 tsunami. The objective of this study was to assess V. marina under five different soil substrates, including a reference treatment (RT) …

Effectiveness Of Tadreb-B1 And 1-Feh W3 Kasp Markers In Spring And Winter Wheat Populations For Marker-Assisted Selection To Improve Drought Tolerance, Shamseldeen Eltaher, Mostafa Hashem, Asmaa A. M. Ahmed, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam

Department of Agronomy and Horticulture: Faculty Publications

Due to the advances in DNA markers, kompetitive allele-specific PCR (KASP) markers could accelerate breeding programs and genetically improve drought tolerance. Two previously reported KASP markers, TaDreb-B1 and 1-FEH w3, were investigated in this study for the markerassisted selection (MAS) of drought tolerance. Two highly diverse spring and winter wheat populations were genotyped using these two KASP markers. The same populations were evaluated for drought tolerance at seedling (drought stress) and reproductive (normal and drought stress) growth stages. The single-marker analysis revealed a high significant association between the target allele of 1-FEH w3 and drought susceptibility in the spring …

Examining Physiological And Photosynthetic Effect Of Led Lighting On Lettuce And Kale Microgreens, James Travis Blanche

Theses and Dissertations

The objective of these studies was to observe the effects of LED lighting treatments on physiological parameters of lettuce and microgreens. Four different lighting treatments; 1) Physiospec (white), 2) Red, 3) DR9 (25:75 Blue:Red), and 4) Anthospec (at 20/80 Blue:Red); were used in this experiment. Two experiments were conducted: 1) Examining the effects of LED lights on lettuce yield and photosynthetic activity and 2) The effects of switching lighting regiments halfway through the growth cycle on Kale microgreen yield and color. Physiospec lighting was found to promote the highest yield and photosynthetic activity among the four treatments for lettuce. It …

The Influence Of Overwatering, Underwatering, And Waterlogging On The Growth Of Kale (Brassica Oleracea Var. Acephala), Skyler R. Brazel

Theses and Dissertations

The Intergovernmental Panel on Climate Change has predicted that there will be increases in precipitation and heat-induced drought events globally. Information on kale response to waterlogging is minimal. The purpose of this project was to identify the response of kale to three treatments of water stress: underwatering, overwatering, and waterlogging. Plant pigments analyzed displayed a varied response to underwatering and overwatering, with concentrations changing with maturity but with reductions and no changes, respectively, at full harvest maturity. Glucosinolate concentrations were also influenced by maturity and increased under waterlogging and underwatering but no differences with overwatering. Overall, water stress to any …

Long-Term Conservation And Conventional Tillage Systems Impact Physical And Biochemical Soil Health Indicators In A Corn–Soybean Rotation, Anuoluwa O. Sangotayo, Jemila Chellappa, Udayakumar Sekaran, Sangeeta Bansal, Padma Angmo, Paul J. Jasa, Sandeep Kumar, Javed Iqbal

Department of Agronomy and Horticulture: Faculty Publications

Agricultural management practices tend to influence soil structure stabilization, mediating many physical, chemical, and biological processes in soils. Therefore, understanding the long-term effects of management practices on various soil health indicators is crucial to develop sustainable agricultural practices. This study aimed to assess the long-term conventional and conservation tillage effects on soil physical (aggregates) and biochemical (soil organic carbon [SOC], enzymes, and microbial biomass) parameters under a range of tillage practices in a corn–soybean rotation in Nebraska. The experiment was conducted at two locations as follows: (1) Concord site (36 years) with the three treatments: no-till (NT), disk, and moldboard …

Hybrid Allele‑Specific Chip‑Seq Analysis Identifies Variation In Brassinosteroid‑Responsive Transcription Factor Binding Linked To Traits In Maize, Thomas Hartwig, Michael Banf, Gisele Passaia Prietsch, Jia‑Ying Zhu, Isabel Mora‑Ramírez, Jos H. M. Schippers, Samantha J. Snodgrass, Arun S. Seetharam, Bruno Huettel, Judith M. Kolkman, Jiashi Yang, Julia Engelhorn, Zhi‑Yong Wang

Department of Agronomy and Horticulture: Faculty Publications

Background: Genetic variation in regulatory sequences that alter transcription factor (TF) binding is a major cause of phenotypic diversity. Brassinosteroid is a growth hormone that has major effects on plant phenotypes. Genetic variation in brassinosteroidresponsive cis-elements likely contributes to trait variation. Pinpointing such regulatory variations and quantitative genomic analysis of the variation in TF-target binding, however, remains challenging. How variation in transcriptional targets of signaling pathways such as the brassinosteroid pathway contributes to phenotypic variation is an important question to be investigated with innovative approaches.

Results: Here, we use a hybrid allele-specific chromatin binding sequencing (HASChseq) approach and …

Comparing Allistic And Autistic Honors Students’ Perceived Barriers To Succeeding In Honors And Their Preferred Resources, Vivian Brantley

Horticulture Undergraduate Honors Theses

In recent years, neurodivergent diagnoses have increased, particularly Autism Spectrum Disorders (ASD). The Center for Disease Control (CDC) reported that as of 2020, among eight-year-olds, one in 36 children were diagnosed with ASD. Among college students with ASD there are many barriers to succeeding in higher education, such as being predisposed to higher drop-out rates than their allistic counterparts. Students with ASD struggle with many basic skills required to succeed in higher education like language processing and executive function. Because of their ASD diagnosis students may also struggle with the additional responsibilities associated with honors programs such as maintaining certain …

Effect Of Nanobubble-Oxygenated Water For Irrigation Of Sand-Based Golf Course Putting Greens, Eric Deboer

Graduate Theses and Dissertations

Nanobubbles (NBs) are sub-micron, stable cavities of gas. The properties of NBs theoretically allow long bubble residence times and high gas dissolution rates resulting in the supersaturation of oxygen in water. Adequate soil aeration is a constant concern for producers of agricultural and horticultural crops. Because of their unique properties, NBs have been investigated for oxygenating water used in the irrigation of various agricultural and horticultural crops to increase soil oxygen concentration. Creeping bentgrass (Agrostis stolonifera L.) is the most widely used turfgrass for golf course putting green surfaces. Golf course superintendents use various methods to increase soil oxygen in …

Nebline, May 2023

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

The 4-H Thriving Model Provides a Guide for Helping Youth Grow to Their Full Potential by Tracy Anderson

Beef: A Healthy and Delicious Choice for Your Next Meal by Kayla Colgrove

Recipe of the Month: Skillet Lasagna by Kayla Colgrove

Managing Chlorosis in Trees by Sarah Browning

Garden Guide: Things to Do This Month

May is the Perfect Time to Learn More About Your Drinking Water! by Becky Schuerman

Cash Rent Survey

Challenging Behavior Corner: Providing Choices by Hayley Jackson

Pollinator Spotlight: The Yucca Moth by Kait Chapman

At Ag Literacy Festival, 4th Graders Learn How Agriculture Impacts Their Daily …

Evaluating Planting Green And Herbicides For Integrated Weed Management And Their Effect On Soil Properties In Corn And Soybean In Nebraska, Trey Parker Stephens

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Producers across the Midwest are finding new ways to implement cover crops into cropping systems and the practice of “Planting Green” is one of the newest uses of cover crops. When planting green, producers plant their row crops into actively growing cover crops and terminate the cover crop at time of planting or shortly after planting. This practice would allow for higher biomass accumulation of the cover crop and could aid in weed management of herbicide-resistant weeds. The objective of the first two studies was to evaluate planting green and its effect on soil-applied residual herbicides, weed management, dicamba/glyphosate-resistant soybean …

Genomic Selection For Yield And Seed Composition Stability In An Applied Soybean Breeding Program, Benjamin Mark Harms

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Stability traits are of primary importance in plant breeding to ensure consistency in phenotype across a range of environments. However, selection efficiency and accuracy for stability traits can be hindered due to the requirement of obtaining phenotype data across multiple years and environments for proper stability analysis. Genomic selection is a method that allows prediction of a phenotype prior to observation in the field using genome-wide marker data and phenotype data from a training population. To assess prediction of stability traits, two elite-yielding soybean populations developed three years apart in the same breeding program were used. The individuals in each …

Influence Of Cover Crops On Soybean Yield And Partial Returns As An Alternative To Double-Crop Soybean In Arkansas, Drew Kirkpatrick, Trenton L. Roberts, Kris Brye, Jeremy Ross

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

The number of hectares dedicated to winter wheat (Triticum aestivum L.) in the southern United States has been trending downward for almost a decade, presenting an opportunity for producers to utilize alternatives in their soybean [Glycine max (L.) Merr.] rotations, such as winter cover crops. A study was established to compare the cover crops to traditional cropping systems by determining the effect each had on a successive soybean crop and to examine partial economic returns. Cropping systems implemented were winter fallow, winter wheat, and seven cover crop treatments, including cereal rye (Secale cereale L.), black-seeded oat ( …

A Genome-Wide Association Study And Genomic Prediction For Phakopsora Pachyrhizi Resistance In Soybean, Haizheng Xiong, Yilin Chen, Yong-Bao Pan, Jinshe Wang, Weiguo Lu, Ainong Shi

Horticulture Faculty Publications and Presentations

Soybean brown rust (SBR), caused by Phakopsora pachyrhizi, is a devastating fungal disease that threatens global soybean production. This study conducted a genome-wide association study (GWAS) with seven models on a panel of 3,082 soybean accessions to identify the markers associated with SBR resistance by 30,314 high quality single nucleotide polymorphism (SNPs). Then five genomic selection (GS) models, including Ridge regression best linear unbiased predictor (rrBLUP), Genomic best linear unbiased predictor (gBLUP), Bayesian least absolute shrinkage and selection operator (Bayesian LASSO), Random Forest (RF), and Support vector machines (SVM), were used to predict breeding values of SBR resistance using …

Meta-Analysis Of The Effects Of Endophytes And Their Secondary Metabolites On Herbivory From Insects, Derrick Taylor

All Theses

Endophyte-infected plants have reduced the level of herbivory. The defense to herbivory can vary due to the metabolites in the plant and the method of feeding from the pest. Secondary metabolites produced by the endophytes are mainly alkaloids. The associations between endophytes and some alkaloid groups are studied in greater detail. The lack of research of certain alkaloid groups ultimately means that some alkaloids had more data that could be analyzed. As a result, the alkaloid groups were not evenly represented in the meta-analysis. The meta-analysis analyzed the relative effectiveness of the metabolites or endophytes in each of the articles …

Multiomic Explorations Of Fruit Texture And Postharvest Quality In Blackberry And Muscadine Grape, Thomas Mason Chizk

Graduate Theses and Dissertations

For most fresh-market fruit crops, texture is an important trait that strongly affects both shipping potential and consumer opinion. Efficient, scalable phenotyping methods are required by breeding programs to effectively select for improvements to fruit texture quality. In muscadine, we have developed a recommendation for characterizing complex muscadine grape texture profiles by comparing the results of breeders’ ratings, descriptive sensory panel results, and an array of instrumental protocols. Regression models were constructed to predict awareness of skins, crispness, hardness, and visual separation explaining 85%, 91%, 82%, and 83% of variance respectively. Genotypes that scored most highly in breeders’ ratings of …

Investigating Effect Of Seed Source And Developing Germination Protocols To Improve Success In Restoration Of Arkansas Tallgrass Prairies, Rhiannon Spencerosa

Graduate Theses and Dissertations

American tallgrass prairie has faced losses estimated at 87-98% of original land area since European settlement. Native seeds are often used to supplement struggling or extinct plant populations in tallgrass prairie restoration and establishment sites. Two main considerations in restoration and establishment are from where to source seed and how to obtain high germination rates. In order to determine the effect of seed source, a common garden experiment was performed in Fayetteville, Arkansas in 2022 with five prairie species: Andropogon gerardii (big bluestem), Bouteloua curtipendula (sideoats grama), Panicum virgatum (switchgrass), Schizachyrium scoparium (little bluestem), and Sorghastrum nutans (Indiangrass). Seed was …

A Deep Learning Framework For Processing And Classification Of Hyperspectral Rice Seed Images Grown Under High Day And Night Temperatures, Víctor Díaz-Martínez, Jairo Orozco-Sandoval, Vidya Manian, Balpreet K. Dhatt, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

A framework combining two powerful tools of hyperspectral imaging and deep learning for the processing and classification of hyperspectral images (HSI) of rice seeds is presented. A seed-based approach that trains a three-dimensional convolutional neural network (3D-CNN) using the full seed spectral hypercube for classifying the seed images from high day and high night temperatures, both including a control group, is developed. A pixel-based seed classification approach is implemented using a deep neural network (DNN). The seed and pixel-based deep learning architectures are validated and tested using hyperspectral images from five different rice seed treatments with six different high temperature …

Entropy And Energy Profiles Of Chemical Reactions, Johannes C. B. Dietschreit, Dennis J. Diestler, Rafael Gómez-Bombarelli

Department of Agronomy and Horticulture: Faculty Publications

The description of chemical processes at the molecular level is often facilitated by use of reaction coordinates, or collective variables (CVs). The CV measures the progress of the reaction and allows the construction of profiles that track the evolution of a specific property as the reaction progresses. Whereas CVs are routinely used, especially alongside enhanced sampling techniques, links between profiles and thermodynamic state functions and reaction rate constants are not rigorously exploited. Here, we report a unified treatment of such reaction profiles. Tractable expressions are derived for the free-energy, internal-energy, and entropy profiles as functions of only the CV. We …

Divergent Metabolic Changes In Rhizomes Of Lowland And Upland Switchgrass (Panicum Virgatum) From Early Season Through Dormancy Onset, Nathan A. Palmer, Gautam Sarath, Michael J. Bowman, Aaron J. Saathoff, Serge J. Edmé, Robert B. Mitchell, Christian M. Tobias, Soundararajan Madhavan, Erin D. Scully, Scott E. Sattler

Department of Agronomy and Horticulture: Faculty Publications

High-biomass-yielding southerly adapted switchgrasses (Panicum virgatum L.) frequently suffer from unpredictable winter hardiness at more northerly sites arising from damage to rhizomes that prevent effective spring regrowth. Previously, changes occurring over the growing season in rhizomes sampled from a cold-adapted tetraploid upland cultivar, Summer, demonstrated a role for abscisic acid (ABA), starch accumulation, and transcriptional reprogramming as drivers of dormancy onset and potential keys to rhizome health during winter dormancy. Here, rhizome metabolism of a high-yielding southerly adapted tetraploid switchgrass cultivar, Kanlow—which is a significant source of genetics for yield improvement—was studied over a growing season at a northern …