Proteomic Profiling Of Maize Opaque Endosperm Mutants
Reveals Selective Accumulation Of Lysine-Enriched Proteins,
2015
University of Nebraska-Lincoln
Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding
Department of Agronomy and Horticulture: Faculty Publications
Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such as protein transport and folding, amino acid biosynthesis, …
Tonoplast Sugar Transporters (Sbtsts) Putatively Control
Sucrose Accumulation In Sweet Sorghum Stems,
2015
University of Missouri
Tonoplast Sugar Transporters (Sbtsts) Putatively Control Sucrose Accumulation In Sweet Sorghum Stems, Saadia Bihmidine, Benjamin T. Julius, Ismail M. Dweikat, David M. Braun
Department of Agronomy and Horticulture: Faculty Publications
Carbohydrates are differentially partitioned in sweet versus grain sorghums. While the latter preferentially accumulate starch in the grain, the former primarily store large amounts of sucrose in the stem. Previous work determined that neither sucrose metabolizing enzymes nor changes in Sucrose transporter (SUT) gene expression accounted for the carbohydrate partitioning differences. Recently, 2 additional classes of sucrose transport proteins, Tonoplast Sugar Transporters (TSTs) and SWEETs, were identified; thus, we examined whether their expression tracked sucrose accumulation in sweet sorghum stems. We determined 2 TSTs were differentially expressed in sweet vs. grain sorghum stems, likely underlying the massive difference in sucrose …
Cover Crops And Ecosystem Services: Insights From Studies In Temperate Soils,
2015
University of Nebraska-Lincoln
Cover Crops And Ecosystem Services: Insights From Studies In Temperate Soils, Humberto Blanco-Canqui, Tim M. Shaver, John L. Lindquist, Charles A. Shapiro, Roger Wesley Elmore, Charles A. Francis, Gary W. Hergert
Department of Agronomy and Horticulture: Faculty Publications
Cover crops (CCs) can provide multiple soil, agricultural production, and environmental benefits. However, a better understanding of such potential ecosystem services is needed. We summarized the current state of knowledge of CC effects on soil C stocks, soil erosion, physical properties, soil water, nutrients, microbial properties, weed control, crop yields, expanded uses, and economics and highlighted research needs. Our review indicates that CCs are multifunctional. Cover crops increase soil organic C stocks (0.1–1 Mg ha–1 yr–1) with the magnitude depending on biomass amount, years in CCs, and initial soil C level. Runoff loss can decrease by up …
Differential Expression Analysis In Rna-Seq By
A Naive Bayes Classifier With Local Normalization,
2015
University of Nebraska-Lincoln
Differential Expression Analysis In Rna-Seq By A Naive Bayes Classifier With Local Normalization, Yongchao Dou, Xiaomei Guo, Lingling Yuan, David R. Holding, Chi Zhang
Department of Agronomy and Horticulture: Faculty Publications
To improve the applicability of RNA-seq technology, a large number of RNA-seq data analysis methods and correction algorithms have been developed. Although these new methods and algorithms have steadily improved transcriptome analysis, greater prediction accuracy is needed to better guide experimental designs with computational results. In this study, a new tool for the identification of differentially expressed genes with RNA-seq data, named GExposer, was developed. This tool introduces a local normalization algorithm to reduce the bias of nonrandomly positioned read depth. The naive Bayes classifier is employed to integrate fold change, transcript length, and GC content to identify differentially expressed …
The Influence Of Production Practices, Tillage, And Endophytic Bacteria On Bell Pepper Productivity And Physiology Under Different Irrigation Regimes,
2015
University of Kentucky
The Influence Of Production Practices, Tillage, And Endophytic Bacteria On Bell Pepper Productivity And Physiology Under Different Irrigation Regimes, Zheng Wang
Theses and Dissertations--Plant and Soil Sciences
To evaluate the strip tillage in organic bell pepper (Capsicum annuum L.) production as an integrated system for sustainable vegetable cropping two-years of field trials were conducted in 2011 and 2012. The field trials were conducted to determine the viability of strip tillage in conventional and organic bell pepper production systems by comparing plant growth, water status, and fruit yield to plastic mulch grown plants application under different irrigation regimes. The two-year field data demonstrated that organic pepper with strip tillage application was a viable combination that produced comparable yield to conventional plastic mulch system and utilized water more …
Conservation Agriculture In Kentucky: Investigating Nitrogen Loss And Dynamics In Corn Systems Following Wheat And Hairy Vetch Cover Crops,
2015
University of Kentucky
Conservation Agriculture In Kentucky: Investigating Nitrogen Loss And Dynamics In Corn Systems Following Wheat And Hairy Vetch Cover Crops, Rebecca Erin Shelton
Theses and Dissertations--Plant and Soil Sciences
Unintentional nitrogen (N) loss from agroecosystems produces greenhouse gases, induces eutrophication, and is costly for farmers; therefore, adoption of conservation agricultural management practices, such as no-till and cover cropping, has increased. This study assessed N loss via leaching, NH3 volatilization, N2O emissions, and N retention in plant and soil pools of corn conservation agroecosystems across a year. Three systems were evaluated: 1) an unfertilized organic system with cover crops Vicia villosa, Triticum aestivum, or a mix of the two; 2) an organic system with a Vicia cover crop employing three fertilization schemes (0 N, organic N, …
Inducing Stress Early And Reducing Stress Late To Increase Soybean (Glycine Max) Yield,
2015
University of Kentucky
Inducing Stress Early And Reducing Stress Late To Increase Soybean (Glycine Max) Yield, Gary L. Gregg
Theses and Dissertations--Plant and Soil Sciences
Relatively little change in national soybean (Glycine max) yield over the previous years have led many farmers to creating management regimes focused on plant stress. Field experiments consisting of two different relative maturity (2.8RM and 4.5 RM) soybean cultivars were established at three locations across Kentucky in 2013 and 2014. Each maturity group received a single application, sequential applications, or a combination of the following treatments: N’N-diformyl urea, lactofen, lambda-cyhalothrin with thiamethoxam, and azoxystrobin with propiconazole. Relative maturity and yield environment*treatment interactions were observed to be significant (p 0≤.05). 4.5 RM soybean cultivars yielded significantly greater (800 kg …
Soil Management And Nitrogen Dynamics In Burley Tobacco Rotations,
2015
University of Kentucky
Soil Management And Nitrogen Dynamics In Burley Tobacco Rotations, Congming Zou
Theses and Dissertations--Plant and Soil Sciences
Agronomic practices, including tillage, crop rotation and N fertilization, have been developed to efficiently manage soil N dynamics and crop N nutrition. These practices can affect soil organic carbon (SOC) and soil total nitrogen (STN) sequestration, and consequently influence soil nitrogen mineralization (SNM) and crop N nutrition. However, little research has been systematically and simultaneously conducted to examine the effect of agronomic management on (1) SOC and STN stocks; (2) SNM; and (3) crop N nutrition. Burley tobacco (Nicotiana tobacum L.) is a N demanding crop and subject to inefficiency in N fertilization. Moreover, conservation tillage and rotation have been …
Evaluating A Satellite-Based Seasonal Evapotranspiration Product And Identifying Its Relationship With Other Satellite-Derived Products And Crop Yield: A Case Study For Ethiopia,
2015
University of Nebraska-Lincoln
Evaluating A Satellite-Based Seasonal Evapotranspiration Product And Identifying Its Relationship With Other Satellite-Derived Products And Crop Yield: A Case Study For Ethiopia, Tsegaye Tadesse, Gabriel B. Senay, Getachew Berhan, Teshome Regassa, Shimelis Beyene
Department of Agronomy and Horticulture: Faculty Publications
Satellite-derived evapotranspiration anomalies and normalized difference vegetation index (NDVI) products from Moderate Resolution Imaging Spectroradiometer (MODIS) data are currently used for African agricultural drought monitoring and food security status assessment. In this study, a process to evaluate satellite-derived evapotranspiration (ETa) products with a geospatial statistical exploratory technique that uses NDVI, satellite-derived rainfall estimate (RFE), and crop yield data has been developed. The main goal of this study was to evaluate the ETa using the NDVI and RFE, and identify a relationship between the ETa and Ethiopia’s cereal crop (i.e., teff, sorghum, corn/maize, barley, and wheat) yields during the main rainy …
Germplasm Enhancement For Resistance To Pyrenophora Tritici-Repentis In Wheat,
2015
Department of Agriculture and Food, Western Australia
Germplasm Enhancement For Resistance To Pyrenophora Tritici-Repentis In Wheat, Manisha Shankar, Diane E. Mather, Dorthe Jorgensen, Hossein Golzar, Ken J. Chalmers, Grant J. Hollaway, Mark S. Mclean, Stephen M. Neate, Rob Loughman
Conference papers and presentations
Yellow spot (syn. tan spot), caused by Pyrenophora tritici-repentis, is an important foliar disease of wheat in Australia that causes losses exceeding 50 % when conditions are favourable for disease development. Although good progress has been made internationally to understand yellow spot resistance, relatively few resistance genes have been identified and mapped in Australian germplasm and only one (tsn1 on chromosome 5BL) is in general and known use in Australian breeding programs. Although tsn1 is an important yellow spot resistance gene, it doesn’t explain the full spectrum of resistance and there is a significant opportunity to enhance expression …
Assessment Of Rice Self-Sufficiency In 2025 In Eight African Countries,
2015
Wageningen University
Assessment Of Rice Self-Sufficiency In 2025 In Eight African Countries, P. A.J. Van Oort, K. Saito, E. Amovin-Assagba, Lenny G.J. Van Bussel, Justin Van Wart, Hugo De Groot, Martin K. Van Ittersum, Kenneth Cassman, M. C.S. Wopereis
Department of Agronomy and Horticulture: Faculty Publications
Most African countries are far from self-sufficient in meeting their rice consumption; in eight countries the production: consumption ratio, ranged from 0.16 to 1.18 in 2012. We show that for the year 2025, with population growth, diet change and yield increase on existing land (intensification), countries cannot become fully self-sufficient in rice. This implies that for the future, a mixture of area expansion and imports will be needed on top of yield gap closure. Further research is needed for identification of most suitable new land for rice area expansion and areas that should be protected.
How Good Is Good Enough? Data Requirements For Reliable Crop Yield Simulations And Yield-Gap Analysis,
2015
University of Nebraska-Lincoln
How Good Is Good Enough? Data Requirements For Reliable Crop Yield Simulations And Yield-Gap Analysis, Patricio Grassini, Lenny G.J. Van Bussel, Justin Van Wart, Joost Wolf, Lieven Claessens, Haishun Yang, Hendrik Boogaard, Hugo De Groot, Martin K. Van Ittersum, Kenneth Cassman
Department of Agronomy and Horticulture: Faculty Publications
Numerous studies have been published during the past two decades that use simulation models to assess crop yield gaps (quantified as the difference between potential and actual farm yields), impact of climate change on future crop yields, and land-use change. However, there is a wide range in quality and spatial and temporal scale and resolution of climate and soil data underpinning these studies, as well as widely differing assumptions about cropping-system context and crop model calibration. Here we present an explicit rationale and methodology for selecting data sources for simulating crop yields and estimating yield gaps at specific locations that …
Selective Genotyping For Marker Assisted Selection Strategies For Soybean Yield Improvement,
2015
Clemson University
Selective Genotyping For Marker Assisted Selection Strategies For Soybean Yield Improvement, Benjamin D. Fallen, Fred L. Allen, Dean A. Kopsell, Arnold M. Saxton, Leah Mchale, J. Grover Shannon, Stella K. Kantartzi, Andrea J. Cardinal, P. B. Cregan, D. L. Hyten, Vincent R. Pantalone
Department of Agronomy and Horticulture: Faculty Publications
Using molecular markers in soybean [Glycine max (L.) Merr.] has lead to the identification of major loci controlling quantitative and qualitative traits that include: disease resistance, insect resistance and tolerance to abiotic stresses. Yield has been considered as one of the most important quantitative traits in soybean breeding. Unfortunately, yield is a very complex trait and most yield quantitative trait loci (QTL) that have been identified have had only limited success for marker assisted selection (MAS). The objective of this study was to identify QTL associated with soybean seed yield in preliminary yield trials grown in different environments and …
Using A Simple Leaf Color Chart To Estimate Leaf
And Canopy Chlorophyll A Content In Maize (Zea
Mays),
2015
University of Nebraska-Lincoln
Using A Simple Leaf Color Chart To Estimate Leaf And Canopy Chlorophyll A Content In Maize (Zea Mays), Anthony Nguy-Robertson, Yi Peng, Timothy Arkebauer, David Scoby, James Schepers, Anatoly Gitelson
Department of Agronomy and Horticulture: Faculty Publications
This study utilized a leaf color chart (LCC) to characterize the variation in leaf chlorophyll and estimate canopy chlorophyll in maize (Zea mays). The LCC consisted of four levels of greenness and was used to sort maize leaves in 2011 for three fields near Mead, Nebraska, USA. Leaf chlorophyll content for each color chart class was determined using two leaf-level sensors. The variation within each LCC class was reasonable (CV < 56%). The darkest color class predominated and indicated adequate fertilization rates using a SPAD. Canopy chlorophyll content was estimated using destructively measured leaf area index (LAI) and the LCC. This approach was verified with a method utilizing canopy reflectance collected by both satellite imagery and a four-band radiometer. The error between the two methods was reasonable (RMSE = 0.55-0.88 g m-2; CV = 25.6-50.4%), indicating that both leaf and canopy chlorophyll can be estimated cheaply without a wet lab or field-based sensors.
Transgenic Wheat Expressing
A Barley Udp-Glucosyltransferase Detoxifies
Deoxynivalenol And Provides High Levels
Of Resistance To Fusarium Graminearum,
2015
University of Minnesota
Transgenic Wheat Expressing A Barley Udp-Glucosyltransferase Detoxifies Deoxynivalenol And Provides High Levels Of Resistance To Fusarium Graminearum, Xi Li, Sanghyun Shin, Shane Heinen, Ruth Dill-Macky, Franz Berthiller, Natalya Nersesian, Thomas Clemente, Susan Mccormick, Gary J. Muehbauer
Department of Agronomy and Horticulture: Faculty Publications
Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is a devastating disease of wheat that results in economic losses worldwide. During infection, F. graminearum produces trichothecene mycotoxins, including deoxynivalenol (DON), that increase fungal virulence and reduce grain quality. Transgenic wheat expressing a barley UDP-glucosyltransferase (HvUGT13248) were developed and evaluated for FHB resistance, DON accumulation, and the ability to metabolize DON to the less toxic DON-3-O-glucoside (D3G). Pointinoculation tests in the greenhouse showed that transgenic wheat carrying HvUGT13248 exhibited significantly higher resistance to disease spread in the spike (type II resistance) compared with nontransformed controls. Two transgenic …
Understanding Rice Adaptation To Varying
Agro-Ecosystems: Trait Interactions And
Quantitative Trait Loci,
2015
International Rice Research Institute
Understanding Rice Adaptation To Varying Agro-Ecosystems: Trait Interactions And Quantitative Trait Loci, Shalabh Dixit, Alexandre Grondin, Cheng-Ruei Lee, Amelia Henry, Thomas-Mitchell Olds, Arvind Kumar
Department of Agronomy and Horticulture: Faculty Publications
Background: Interaction and genetic control for traits influencing the adaptation of the rice crop to varying environments was studied in a mapping population derived from parents (Moroberekan and Swarna) contrasting for drought tolerance, yield potential, lodging resistance, and adaptation to dry direct seeding. A BC2F3-derived mapping population for traits related to these four trait groups was phenotyped to understand the interactions among traits and to map and align QTLs using composite interval mapping (CIM). The study also aimed to identify QTLs for the four trait groups as composite traits using multivariate least square interval mapping (MLSIM) …
Overexpression Of Sbmyb60 Impacts Phenylpropanoid
Biosynthesis And Alters Secondary Cell Wall Composition In
Sorghum Bicolor,
2015
University of Nebraska-Lincoln
Overexpression Of Sbmyb60 Impacts Phenylpropanoid Biosynthesis And Alters Secondary Cell Wall Composition In Sorghum Bicolor, Erin D. Scully, Tammy Gries, Gautam Sarath, Nathan A. Palmer, Lisa Baird, Michelle J. Serapiglia, Bruce S. Dien, Akwasi A. Boateng, Zhengxiang Ge, Deanna L. Funnell-Harris, Paul Twigg, Thomas E. Clemente, Scott E. Sattler
Department of Agronomy and Horticulture: Faculty Publications
The phenylpropanoid biosynthetic pathway that generates lignin subunits represents a significant target for altering the abundance and composition of lignin. The global regulators of phenylpropanoid metabolism may include MYB transcription factors, whose expression levels have been correlated with changes in secondary cell wall composition and the levels of several other aromatic compounds, including anthocyanins and flavonoids. While transcription factors correlated with downregulation of the phenylpropanoid biosynthesis pathway have been identified in several grass species, few transcription factors linked to activation of this pathway have been identified in C4 grasses, some of which are being developed as dedicated bioenergy feedstocks. In …
A Dedicated Type Ii Nadph Dehydrogenase Performs The
Penultimate Step In The Biosynthesis Of Vitamin K1 In
Synechocystis And Arabidopsis,
2015
University of Nebraska–Lincoln
A Dedicated Type Ii Nadph Dehydrogenase Performs The Penultimate Step In The Biosynthesis Of Vitamin K1 In Synechocystis And Arabidopsis, Abdelhak Fatihi, Scott Latimer, Stefan Schmollinger, Anna Block, Patrick Dussault, Wim F.J. Vermaas, Sabeeha S. Merchant, Gilles J. Basset
Department of Agronomy and Horticulture: Faculty Publications
Mutation of Arabidopsis thaliana NAD(P)H DEHYDROGENASE C1 (NDC1; At5g08740) results in the accumulation of demethylphylloquinone, a late biosynthetic intermediate of vitamin K1. Gene coexpression and phylogenomics analyses showed that conserved functional associations occur between vitamin K biosynthesis and NDC1 homologs throughout the prokaryotic and eukaryotic lineages. Deletion of Synechocystis ndbB, which encodes for one such homolog, resulted in the same defects as those observed in the cyanobacterial demethylnaphthoquinone methyltransferase knockout. Chemical modeling and assay of purified demethylnaphthoquinone methyltransferase demonstrated that, by virtue of the strong electrophilic nature of S-adenosyl-Lmethionine, the transmethylation of the demethylated precursor …
Aquaporins Contribute To Aba-Triggered Stomatal Closure
Through Ost1-Mediated Phosphorylation,
2015
University of Nebraska-Lincoln
Aquaporins Contribute To Aba-Triggered Stomatal Closure Through Ost1-Mediated Phosphorylation, Alexandre Grondin, Olivier Rodrigues, Lionel Verdoucq, Sylvain Merlot, Nathalie Leonhardt, Christophe Maurel
Department of Agronomy and Horticulture: Faculty Publications
Stomatal movements in response to environmental stimuli critically control the plant water status. Although these movements are governed by osmotically driven changes in guard cell volume, the role of membrane water channels (aquaporins) has remained hypothetical. Assays in epidermal peels showed that knockout Arabidopsis thaliana plants lacking the Plasma membrane Intrinsic Protein 2;1 (PIP2;1) aquaporin have a defect in stomatal closure, specifically in response to abscisic acid (ABA). ABA induced a 2-fold increase in osmotic water permeability (Pf) of guard cell protoplasts and an accumulation of reactive oxygen species in guard cells, which were both abrogated in …
Selection For Silage Yield And Composition Did Not
Affect Genomic Diversity Within The Wisconsin
Quality Synthetic Maize Population,
2015
University of Nebraska-Lincoln
Selection For Silage Yield And Composition Did Not Affect Genomic Diversity Within The Wisconsin Quality Synthetic Maize Population, Aaron Lorenz, Timothy M. Beissinger, Renato Rodrigues Silva, Natalia De Leon
Department of Agronomy and Horticulture: Faculty Publications
Maize silage is forage of high quality and yield, and represents the second most important use of maize in the United States. The Wisconsin Quality Synthetic (WQS) maize population has undergone five cycles of recurrent selection for silage yield and composition, resulting in a genetically improved population. The application of high-density molecular markers allows breeders and geneticists to identify important loci through association analysis and selection mapping, as well as to monitor changes in the distribution of genetic diversity across the genome. The objectives of this study were to identify loci controlling variation for maize silage traits through association analysis …
