Physiology Commons^™

Osteoblast Oncostatin M Signaling In Modeled Spaceflight, Jacob Goyden

Boise State University Theses and Dissertations

Bone deterioration is a challenge in long-term spaceflight with significant connections to terrestrial disuse bone loss. Prolonged unloading and radiation exposure, defining characteristics of space travel, have both been associated with changes in inflammatory signaling via IL-6 class cytokines in bone. While there is also some evidence for perturbed IL-6 class signaling in spaceflight, there has been scant examination of the connections between free fall, radiation, and inflammatory stimuli in bone. Our lab and others have shown that the IL-6 class cytokine oncostatin M (OSM) is an important regulator of bone remodeling. We hypothesize that spaceflight alters osteoblast OSM signaling, …

Neutral Sphingomyelinase-3 Mediates Tnf-Stimulated Oxidant Activity In Skeletal Muscle, Jennifer S. Moylan, Jeffrey D. Smith, Erin M. Wolf Horrell, Julie B. Mclean, Gergana M. Deevska, Mark R. Bonnell, Mariana N. Nikolova‑Karakashian, Michael B. Reid

Physiology Faculty Publications

AIMS: Sphingolipid and oxidant signaling affect glucose uptake, atrophy, and force production of skeletal muscle similarly and both are stimulated by tumor necrosis factor (TNF), suggesting a connection between systems. Sphingolipid signaling is initiated by neutral sphingomyelinase (nSMase), a family of agonist-activated effector enzymes. Northern blot analyses suggest that nSMase3 may be a striated muscle-specific nSMase. The present study tested the hypothesis that nSMase3 protein is expressed in skeletal muscle and functions to regulate TNF-stimulated oxidant production.

RESULTS: We demonstrate constitutive nSMase activity in skeletal muscles of healthy mice and humans and in differentiated C2C12 myotubes. nSMase3 ( …

Metagenomic Identification Of A Novel Salt Tolerance Gene From The Human Gut Microbiome Which Encodes A Membrane Protein With Homology To A Brp/Blh-Family Beta-Carotene 15,15'-Monooxygenase, Eamonn P. Culligan, Roy D. Sleator, Julian R. Marchesi, Colin Hill

Department of Biological Sciences Publications

The human gut microbiome consists of at least 3 million non-redundant genes, 150 times that of the core human genome. Herein, we report the identification and characterisation of a novel stress tolerance gene from the human gut metagenome. The locus, assigned brpA, encodes a membrane protein with homology to a brp/blh-family β-carotene monooxygenase. Cloning and heterologous expression of brpA in Escherichia coli confers a significant salt tolerance phenotype. Furthermore, when cultured in the presence of exogenous β-carotene, cell pellets adopt a red/orange pigmentation indicating the incorporation of carotenoids in the cell membrane.

The Effect Of Angiotensin Converting Enzyme Genotype On Aerobic Capacity Following High Intensity Interval Training, Nicole Goddard, Matthew Baker, Tim Higgins, Christian Cobbold

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 250-259, 2014. Obesity increases the risk of developing type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD). Physical activity can reduce T2DM and CVD risk, and increase aerobic capacity, a significant predictor of all-cause mortality and morbidity. High intensity interval training (HIIT) produces similar improvements in aerobic capacity to continuous moderate exercise (CME). Different genotypes of angiotensin converting enzyme (ACE) have been implicated in improving aerobic capacity and therefore predicted health. This study investigated the effects of different ACE genotypes on the impact of 6 weeks of HIIT on aerobic capacity, and thus …

Contributions Of After School Programs To The Development Of Fundamental Movement Skills In Children, E Jean Burrows, Melanie R. Keats, Angela M. Kolen

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 236-249, 2014. Fundamental movement skill (FMS) proficiency or the ability to perform basic skills (e.g., throwing, catching and jumping) has been linked to participation in lifelong physical activity. FMS proficiency amongst children has declined in the previous 15 years, with more children performing FMS at a low-mastery level. These declines may help explain the insufficient levels of participation in health promoting physical activity seen in today’s youth. The after school time period (e.g., 3 to 6 p.m.), is increasingly considered an opportune time for physical activity interventions. To date, little research has examined …

The Energy Expenditure Of Recreational Ballroom Dance, D. E. Lankford, T. W. Bennion, J. King, N. Hessing, L Lee, D. P. Heil

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 228-235, 2014. The popularity of recreational ballroom dancing has increased dramatically in recent years. Yet, relatively little information is known regarding the physiological demands of ballroom dancing. The purpose of this study was to determine the energy requirements for recreational ballroom dancing. 24 participants volunteered including 12 women (mean ± SD: 21 ± 3 yrs, 165.8 ± 7.4 cm, 56.8 ± 11.1 kg) and 12 men (23 ± 1 yr, 175.5 ± 8.4 cm, 78.1 ± 15.6 kg). Gas exchange was recorded using a portable metabolic system during a series of five ballroom …

The Influence Of Exercise Environment And Gender On Mood And Exertion, Thomas G. Plante Phd, Abpp, Marily A. Opezzo Phd, L. Aislinn Diaz, Selena Pistoresi, Michael Santos, Jacqueline E. Fahey, Elizabeth Kay, Briana Britton, Suheel Khan

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 220-227, 2014. This study examined the influence of exercise environment and gender on post-exercise mood and exertion. College student participants (55 females, 49 males) were instructed to pedal a stationary bike at a moderate pace for 20 minutes. Participants were randomly assigned to one of three laboratory conditions: (1) exercising in front of a mirror and posters showing ideal fit body types (i.e., celebrity male and female personal trainers), (2) exercising in front of a mirror only, or (3) a control condition in which participants exercised without a mirror or posters. The Activation-Deactivation …

Acute Post-Activation Potentiation In Ncaa Division Ii Female Athletes, Kaiya Senn Sygulla, Charles J. Fountaine

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 212-219, 2014. Post-activation potentiation (PAP) is a phenomenon in which the power output of a muscle is immediately enhanced after heavy resistance exercise. Whereas the majority of PAP research has examined males, minimal research exists as to how female athletes respond. Therefore, the purpose of this study was to investigate the acute PAP response of back squats on static squat jump (SSJ) performance among NCAA Division II female athletes. Female athletes (n=29) who were current roster members from basketball, softball, and volleyball, performed 3 SSJ prior to 3 repetitions of the back squat …

An Acute Bout Of Self-Myofascial Release In The Form Of Foam Rolling Improves Performance Testing, Corey A. Peacock, Darren D. Krein, Tobin A. Silver, Gabriel J. Sanders, Kyle-Patrick A. Von Carlowitz

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 202-211, 2014. Recent developments in the strength and conditioning field have shown the incorporation of foam rolling self-myofascial release in adjunct with a dynamic warm-up. This is thought to improve overall training performance; however, minimal research exists supporting this theory. Therefore, determining if an acute bout of foam rolling self-myofascial release in addition to a dynamic warm-up could influence performance is of importance. In order to do so, eleven athletically trained male subjects participated in a two condition, counterbalanced, crossover within-subjects study comparing two particular warm-up routines. The two warm-up routines compared were …

The Physiologic And Behavioral Implications Of Playing Active And Sedentary Video Games In A Seated And Standing Position, Gabriel J. Sanders, Michael Rebold, Corey A. Peacock, Megan L. Williamson, Antonio Santo, Jacob E. Barkley

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 194-201, 2014. Previous studies have assessed physiologic response while playing video games per manufacturer instructions with participants standing during active video game play and seated during sedentary game play. It is not known whether an assigned seated or standing position affects positional preference and oxygen consumption (VO2) while gaming. The purpose of the study was to assess VO2 and preference of playing active and sedentary video games in a seated and standing position. VO2 was assessed in 25 participants during four, 20-minute conditions; resting, PlayStation 2 Madden NFL Football 2011, Nintendo Wii-Sports Boxing …

Effect Of Long-Term Physical Exercise On Blood Pressure In African Americans, Taunjah P. Bell, Katharine A. Mcintyre, Rosamary Hadley

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 186-193, 2014. Long-term physical exercise has been shown to noticeably reduce blood pressure (BP) and remarkably attenuate symptoms of hypertension. It is believed that physical exercise induces these beneficial effects by increasing the blood supply to the brain, enhancing the release of growth factors from skeletal muscles into the bloodstream, facilitating neurogenesis, stimulating angiogenesis, and influencing endothelial cell proliferation and subsequent endothelial cell membrane permeability. Previous findings also revealed that physical exercise significantly elevates brain-derived neurotrophic factor (BDNF) concentrations which appear to increase dramatically in BP-sensing neurons during hypertension. Elevating BDNF levels is …

Cardiovascular And Metabolic Demands Of The Kettlebell Swing Using A Tabata Interval Versus A Traditional Resistance Protocol, Howard A. Fortner, Jeanette Salgado, Angelica M. Holmstrup, Michael E. Holmstrup Phd

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 179-185, 2014. Tabata (TAB) training, consisting of eight cycles of 20 seconds of maximal exercise followed by 10 seconds of rest, is time-efficient, with aerobic and anaerobic benefit. This study investigated the cardiovascular and metabolic demands of a TAB versus traditional (TRAD) resistance protocol with the kettlebell swing. Fourteen young (18-25y), non-obese (BMI 25.7±0.8 kg/m²) participants reported on three occasions. All testing incorporated measurements of HR, oxygen consumption, and blood lactate accumulation. Each participant completed Tabata kettlebell swings (male- 8kg, female- 4.5kg; 8 intervals; 20s maximal repetitions, 10s rest). On a …

Is There An Optimal Whole-Body Vibration Exposure ‘Dosage’ For Performance Improvement?, R Hugh Morton, Darryl J. Cochrane

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 169-178, 2014. Whole-body vibration exposure has been shown to improve performance in vertical jumping and knee extensions. Some studies have addressed the question of dose optimality, but are inconclusive and inappropriately designed. Our purpose was to more thoroughly seek an optimum combination of duration, amplitude and frequency of exposure to side-alternating whole-body vibration. We used experimental designs constructed for response surface fitting and optimisation, using both blocked and unblocked second order central composite designs with 12 participants. Immediately after each exposure a discomfort index was recorded, then peak and average torque, peak and …

Targeting Of Receptors For Advanced Glycation End-Products (Rage) Diminishes Acute Secondhand Smoke-Induced Inflammation In Mice, Tyler Thomas Wood

Theses and Dissertations

The receptor for advanced glycation end-products (RAGE) has increasingly been demonstrated to be an important modulator of inflammation in cases of pulmonary disease. Published reports involving tobacco smoke exposure have demonstrated increased expression of RAGE, its participation in pro-inflammatory signaling and its role in irreversible pulmonary remodeling. The current research evaluated for the first time the in vivo effects of short-term tobacco smoke exposure in RAGE null and control mice compared to identical animals exposed to room air only. Quantitative real time PCR, immunoblotting, and immunohistochemistry revealed elevated RAGE expression in controls after four weeks of exposure and an anticipated …

Clinical Investigation Of Plethysmographic Variability Index: A Derivative Index Of Pulse Oximetry In Anesthetized Dogs, Vaidehi V. Paranjape

Open Access Theses

Plethysmographic Variability Index (PVI) is a derivative index of pulse oximetry that allows evaluating an individual's intravascular volume status. Perfusion Index (PI) represents the strength of pulse signal at the anatomic site of measurement from which PVI is calculated using changes in PI over respiratory cycles. Plethysmographic Variability Index has been used to detect hypovolemia and predict fluid responsiveness in mechanically ventilated human patients however, fewer studies are available in spontaneously breathing patients. The use of PVI has not been explored extensively in dogs so far. The goals of this study were to establish a common range for PVI and …

Transcriptome Of The Female Synganglion Of The Black-Legged Tick Ixodes Scapularis (Acari: Ixodidae) With Comparison Between Illumina And 454 Systems, Noble Egekwu, Daniel E. Sonenshine, Brooke W. Bissinger, R. Michael Roe

Biological Sciences Faculty Publications

Illumina and 454 pyrosequencing were used to characterize genes from the synganglion of female Ixodes scapularis. GO term searching success for biological processes was similar for samples sequenced by both methods. However, for molecular processes, it was more successful for the Illumina samples than for 454 samples. Functional assignments of transcripts predicting neuropeptides, neuropeptide receptors, neurotransmitter receptors and other genes of interest was done, supported by strong e-values (<-6), and high consensus sequence alignments. Transcripts predicting 15 putative neuropeptide prepropeptides ((allatostatin, allatotropin, bursicon α, corticotropin releasing factor (CRF), CRF-binding protein, eclosion hormone, FMRFamide, glycoprotein A, insulin-like peptide, ion transport peptide, myoinhibitory peptide, inotocin ( = neurophysin-oxytocin), Neuropeptide F, sulfakinin and SIFamide)) and transcripts predicting receptors for 14 neuropeptides (allatostatin, calcitonin, cardioacceleratory peptide, corazonin, CRF, eclosion hormone, gonadotropin-releasing hormone/AKH-like, insulin-like peptide, neuropeptide F, proctolin, pyrokinin, SIFamide, sulfakinin and tachykinin) are reported. Similar to Dermacentor variabilis, we found transcripts matching pro-protein convertase, essential for converting neuropeptide hormones to their mature form. Additionally, transcripts predicting 6 neurotransmitter/neuromodulator receptors (acetylcholine, GABA, dopamine, glutamate, octopamine and …

The Effects Of Squat Depth On Lower Extremity Joint Kinetics, Acl Loading, Pcl Loading, Tibiofemoral Compressive Forces And Activations Of Eight Lower Extremity Muscles, Zachary A. Sievert

Human Movement Studies & Special Education Theses & Dissertations

Performing resistance training exercises are important in many facets oflife. Resistance training can be used from elite athletes preparing for the Olympics to the average person exercising for overall health. The squat is a common training exercise that can be utilized to improve lower extremity strength and performance. There has been much debate on the effects of squatting depth on lower extremity joint kinetics and kinematics. Therefore the purpose of this thesis is to examine the effects of depth and load on the muscle activation of eight lower extremity muscles and three-dimensional (30) joint moments about the right knee, hip …

Motor Cortex Electrical Stimulation Augments Sprouting Of The Corticospinal Tract And Promotes Recovery Of Motor Function, Jason B. Carmel, John H. Martin

Publications and Research

The corticospinal system—with its direct spinal pathway, the corticospinal tract (CST) – is the primary system for controlling voluntary movement. Our approach to CST repair after injury in mature animals was informed by our finding that activity drives establishment of connections with spinal cord circuits during postnatal development. After incomplete injury in maturity, spared CST circuits sprout, and partially restore lost function. Our approach harnesses activity to augment this injury-dependent CST sprouting and to promote function. Lesion of the medullary pyramid unilaterally eliminates all CST axons from one hemisphere and allows examination of CST sprouting from the unaffected hemisphere. We …

Targeting The Wnt/Β-Catenin Signaling Pathway In Liver Cancer Stem Cells And Hepatocellular Carcinoma Cell Lines With Fh535, Roberto Gedaly, Roberto Galuppo, Michael F. Daily, Malay B. Shah, Erin Colleen Maynard, Changguo Chen, Xiping Zhang, Karyn A. Esser, Donald A. Cohen, B. Mark Evers, Jieyun Jiang, Brett T. Spear

Surgery Faculty Publications

Activation of the Wnt/β-catenin pathway has been observed in at least 1/3 of hepatocellular carcinomas (HCC), and a significant number of these have mutations in the β-catenin gene. Therefore, effective inhibition of this pathway could provide a novel method to treat HCC. The purposed of this study was to determine whether FH535, which was previously shown to block the β-catenin pathway, could inhibit β-catenin activation of target genes and inhibit proliferation of Liver Cancer Stem Cells (LCSC) and HCC cell lines. Using β-catenin responsive reporter genes, our data indicates that FH535 can inhibit target gene activation by endogenous and exogenously …

Trnas As Regulators Of Biological Processes, Medha Raina, Michael Ibba

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Transfer RNAs (tRNA) are best known for their role as adaptors during translation of the genetic code. Beyond their canonical role during protein biosynthesis, tRNAs also perform additional functions in both prokaryotes and eukaryotes for example in regulating gene expression. Aminoacylated tRNAs have also been implicated as substrates for non-ribosomal peptide bond formation, post-translational protein labeling, modification of phospholipids in the cell membrane, and antibiotic biosyntheses. Most recently tRNA fragments, or tRFs, have also been recognized to play regulatory roles. Here, we examine in more detail some of the new functions emerging for tRNA in a variety of cellular processes …