Microbiology Commons^™

Hsv-1 Replication In Different Raw 264.7 And J774.1 Macrophage Phenotypes And Macrophage Viability Following Hsv-1 Infection, Yousef Nifaj Alanazi

Browse all Theses and Dissertations

HSV-1 is a ubiquitous virus capable of causing lifelong latent infection. The virus contains a large double strand DNA genome covered by an icosahedral capsid. HSV-1 is a cytolytic virus which can cause a lethal infection. The virus possesses critical protein ICP0 which is capable of interfering with host cell signaling and eventually prevent cell apoptosis. Innate immunity plays a crucial role in defense against HSV-1 infection and macrophage plays a significant role in the innate immune system. Macrophage cells can alter their behavior depending upon certain stimuli and tissue environments. Naive macrophage (M0) cells can be polarized to a …

The Impact Of Cytokines And Hsv-1 On Rab5 Protein Expression, F-Actin Cytoskeleton Rearrangement, And Cell Viability Of Uninfected And Virus-Infected M0, M1, And M2 Raw264.7 Murine Macrophages, Muhannad Falah Alruwaili

Browse all Theses and Dissertations

The endocytic pathway in all eukaryotic cells is necessary to maintain cellular functions, such as initiating transport of intracellular cargos and ingesting pathogens. The main regulator of this process is a member of small GTPase family, Rab5 protein. Rab5 protein plays a key role in the endocytic dynamic delivery of molecules, receptors, and pathogens from the cell membrane to cytoplasmic vesicles.as well as in the exocytic delivery of cellular products to the cell's exterior (Bonifacino & Glick, 2004). Many pathogens have exploited this protein to enter cells. Herpes Simplex Virus Type 1 (HSV-1) enters most cells by fusion or utilizes …

Neurological Involvement Associated With Plasmodium Vivax Malaria From Pakistan, Yousaf Abdullah Khan, Usman Hameed Mian, Najia Karim Ghanchi, Ali Bin Sarwar Zubairi, Mohammad Asim Beg

Department of Pathology and Laboratory Medicine

Plasmodium vivax is the most common specie causing malaria outside Africa with approximately 13.8 million reported cases worldwide. We report case of P. vivax infection with cerebral involvement. A nine year old boy presented with high grade fever accompanied by projectile vomiting and abnormal behavior later he developed seizures, shock, and unconsciousness. P. vivax monoinfection was diagnosed based on peripheral smears and PCR. After antimalarial therapy, patient made full recovery. Current case highlights increasing trend of cerebral complications caused by P. vivax.

High Heterotrophic Counts In Potable Water And Antimicrobial Resistance Among Indicator Organisms In Two Peri-Urban Communities Of Karachi, Pakistan, Sadia Shakoor, Imran Ahmed, Saima Mukhtiar, Israr Ahmed, Farzeen Hirani, Shazia Sultana, Rumina Hasan

Department of Pathology and Laboratory Medicine

Objective: Fecal contamination of potable water leads to unsafe water supply. Although many urban areas of large metropolitan cities receive safe water, peri-urban areas are often not monitored by public health authorities and water supply and quality remain unknown. We assessed microbiological quality and rates of antimicrobial resistance in viable indicator bacteria in two peri-urban communities of Karachi, Pakistan. Water samples were collected over 5 months (October 2015 to February 2016) from these peri-urban communities and samples were processed for microbiological quality as per Standing Committee of Analysts, United Kingdom and World Health Organization guidelines and criteria for drinking water. …

Fourier Transform Infrared Spectroscopy For Analysis Of Kidney Stones, Aysha Habib Khan, Sheharbano Imran, Jamsheer Talati, Lena Jafri

Department of Pathology and Laboratory Medicine

Purpose:
To compare the results of a chemical method of kidney stone analysis with the results of Fourier transform infrared (FT-IR) spectroscopy.
Materials and Methods:
Kidney stones collected between June and October 2015 were simultaneously analyzed by chemical and FT-IR methods.
Results:
Kidney stones (n=449) were collected from patients from 1 to 81 years old. Most stones were from adults, with only 11.5% from children (aged 3-16 years) and 1.5% from children aged adults, the calcium oxalate stone type, calcium oxalate monohydrate (COM, n=224), was the most common crystal, followed by uric acid and calcium oxalate dihydrate (COD, n=83). In …

Pulmonary Alveolar Proteinosis- A Case Report, Renee M. Horner, Douglas S. Gardenhire, Ralph D. Zimmerman

Internet Journal of Allied Health Sciences and Practice

ABSTRACT

Background: Pulmonary Alveolar Proteinosis (PAP) is a rare pulmonary disorder caused by a congregation of excessive lipoproteinaceous material in the alveolar spaces due to impaired surfactant metabolism. The congregation of the protein in the alveolar space leads to difficulty in breathing, impaired pulmonary immunity, and susceptibility to both opportunistic and acquired pulmonary infections. Although Pulmonary Alveolar Proteinosis is rare, there are potential treatments. Whole-lung lavage is the most widely accepted therapy and course of treatment. An additional form of therapy, GM-CSF stimulating therapy, uses recombinant deoxyribonucleic acid (DNA) technology to increase white cell production.

Purpose: The purpose …

Metatranscriptome Of Human Faecal Microbial Communities In A Cohort Of Adult Men, Galeb S. Abu-Ali, Raaj S. Mehta, Jason Lloyd-Price, Himel Mallick, Tobyn Branck, Kerry L. Ivey, David A. Drew, Casey Dulong, Eric Rimm, Jacques Izard, Andrew T. Chan, Curtis Huttenhower

Department of Food Science and Technology: Faculty Publications

The gut microbiome is intimately related to human health, but it is not yet known which functional activities are driven by specific microorganisms' ecological configurations or transcription. We report a large-scale investigation of 372 human fecal metatranscriptomes and 929 metagenomes from a subset of 308 men in the Health Professionals Follow-Up Study. We identified a metatranscriptomic 'core' universally transcribed over time and across participants, often by different microorganisms. In contrast to the housekeeping functions enriched in this core, a 'variable' metatranscriptome included specialized pathways that were differentially expressed both across participants and among microorganisms. Finally, longitudinal metagenomic profiles allowed ecological …

Brain Is A Potential Sanctuary For Subtype C Hiv-1 Irrespective Of Art Treatment Outcome, For Yue Tso, Guobin Kang, Eun Hee Kwon, Peter Julius, Qingsheng Li, John T. West, Charles Wood

Nebraska Center for Virology: Faculty Publications

Subtype C HIV-1 is responsible for the largest proportion of people living with HIV-1 infection. However, there is limited information about the roles of the brain and its cell types as a potential sanctuary for this subtype and how the sanctuary may be affected by the administration of anti-retroviral therapy (ART). To address this issue, we collected postmortem brain tissues from ART treated HIV-1 infected Zambian individuals who experienced complete viral suppression and those who did not. Tissues from various brain compartments were collected from each individual as frozen and formalin-fixed paraffin embedded brain specimens, for detection and quantification of …

Brain Is A Potential Sanctuary For Subtype C Hiv-1 Irrespective Of Art Treatment Outcome, For Yue Tso, Guobin Kang, Peter Julius, Qingsheng Li, John T. West, Charles Wood

Nebraska Center for Virology: Faculty Publications

Subtype C HIV-1 is responsible for the largest proportion of people living with HIV-1 infection. However, there is limited information about the roles of the brain and its cell types as a potential sanctuary for this subtype and how the sanctuary may be affected by the administration of anti-retroviral therapy (ART). To address this issue, we collected postmortem brain tissues from ART treated HIV-1 infected Zambian individuals who experienced complete viral suppression and those who did not. Tissues from various brain compartments were collected from each individual as frozen and formalin-fixed paraffin embedded brain specimens, for detection and quantification of …

Contribution Of The Gp120 V3 Loop To Envelope Glycoprotein Trimer Stability In Primate Immunodeficiency Viruses, Dane Bowder, Haley Hollingsead, Kate Durst, Duoyi Hu, Wenzhong Wei, Joshua Wiggins, Halima Medjahed, Andrés Finzi, Joseph Sodroski, Shi-Hua Xiang

Nebraska Center for Virology: Faculty Publications

The V3 loop of the human immunodeficiency virus type 1 (HIV-1) gp120 exterior envelope glycoprotein (Env) becomes exposed after CD4 binding and contacts the coreceptor to mediate viral entry. Prior to CD4 engagement, a hydrophobic patch located at the tip of the V3 loop stabilizes the non-covalent association of gp120 with the Env trimer of HIV-1 subtype B strains. Here, we show that this conserved hydrophobic patch (amino acid residues 307, 309 and 317) contributes to gp120-trimer association in HIV-1 subtype C, HIV-2 and SIV. Changes that reduced the hydrophobicity of these V3 residues resulted in increased gp120 shedding and …

Ultrastructure Of Meelsvirus: A Nuclear Virus Of Arrow Worms (Phylum Chaetognatha) Producing Giant "Tailed" Virions, George L. Shinn, Brianna L. Bullard

Nebraska Center for Virology: Faculty Publications

Most known giant viruses, i.e., viruses producing giant virions, parasitize amoebae and other unicellular eukaryotes. Although they vary in the level of dependence on host nuclear functions, their virions self-assemble in the host cell's cytoplasm. Here we report the discovery of a new prototype of giant virus infecting epidermal cells of the marine arrow worm Adhesisagitta hispida. Its 1.25 μm-long virions self-assemble and accumulate in the host cell's nucleus. Conventional transmission electron microscopy reveals that the virions have a unique bipartite structure. An ovoid nucleocapsid, situated in a broad "head" end of the virion is surrounded by a thin …

Synthesis, Secretion, And Perception Of Abscisic Acid Regulates Stress Responses In Chlorella Sorokiniana, Maya Khasin, Rebecca E. Cahoon, Sophie Alvarez, Richard Beckeris, Seong-Il Eyun, Qidong Jia, Jean-Jack Riethoven, Kenneth Nickerson, Wayne R. Riekhof

Kenneth Nickerson Papers

Abscisic acid (ABA) is a phytohormone that has been extensively characterized in higher plants for its roles in seed and bud dormancy, leaf abscission, and stress responses. Genomic studies have identified orthologs for ABA-related genes throughout the Viridiplantae, including in unicellular algae; however, the role of ABA in algal physiology has not been characterized, and the existence of such a role has been a matter of dispute. In this study, we demonstrate that ABA is involved in regulating algal stress responses. Chlorella sorokiniana strain UTEX 1230 contains genes orthologous to those of higher plants which are essential for ABA …

Bacterial Inoculants, Endophytic Bacteria And Their Influence On Nicotiana Physiology, Development And Microbiome, Andrea Marisa Sanchez Barrios

Theses and Dissertations--Plant and Soil Sciences

Soil and root microbial communities have been studied for decades, and the incorporation of high-throughput techniques and analysis has allowed the identification of endophytic/non-culturable organisms. This has helped characterize and establish the core microbiome of many model plant species which include underground and aboveground organs. Unfortunately, the information obtained from some of these model plants is not always transferable to other agronomic species. In this project, we decided to study the microbiome of the Nicotiana genus because of its importance in plant physiological and plant-microbe interactions studies. The data obtained was used as baseline information that allowed us to better …

Gene Expression And Lipid Accumulation In Green Microalga Chlamydomonas Reinhardtii (137c) Under Salt Stress Condition, Thanapa Atikij

Chulalongkorn University Theses and Dissertations (Chula ETD)

Biodiesel is an important alternative energy that can reduce CO2 emission, the main cause of Global warming. Algal biomass has been considered to be the third generation biofuels. One of the important strategies that enhance lipid accumulation; precursor of biodiesel production, is stress cultivation. High salt concentration is one of a potential approach to enhance lipid accumulation in microalgae. Here, we investigated the impacts of salt on lipid accumulation in green microalga Chlamydomonas reinhardtii (137c). The results revealed that higher concentrations of NaCl, KCl, and LiCl led to increasing of saturated fatty acid contents for 1.8, 1.2, and 1.1 folds …

Investigation Of Antiviral Molecules Inhibiting Viral Infection, Sitthichai Kanokudom

Chulalongkorn University Theses and Dissertations (Chula ETD)

No abstract provided.

Microbiology: A Laboratory Experience, Holly Ahern

Milne Open Textbooks

As a group of organisms that are too small to see and best known for being agents of disease and death, microbes are not always appreciated for the numerous supportive and positive contributions they make to the living world. Designed to support a course in microbiology, Microbiology: A Laboratory Experience permits a glimpse into both the good and the bad in the microscopic world. The laboratory experiences are designed to engage and support student interest in microbiology as a topic, field of study, and career.

This text provides a series of laboratory exercises compatible with a one-semester undergraduate microbiology or …

A Link Between Gut Microbes & Depression: Microbial Activation Of The Human Kynurenine Pathway, Christina Cobb

CMC Senior Theses

Our gut microbiota is involved in human development, nutrition, and the pathogenesis of gut disorders, but has more recently been implicated as a possible mechanism in the pathophysiology of several brain disorders, including disorders of mood and affect, such as depression. Researchers have referred to this dynamic, bidirectional signaling pathway between the gut and the brain as the “gut-brain axis.” However, most research on this axis has been limited to rodent studies, and there has been little insight into the mechanism behind it. I propose that the kynurenine pathway, where tryptophan is converted to kynurenine, is a compelling mechanism mediating …

Nevada Desert Dust With Heavy Metals Suppresses Igm Antibody Production, Deborah E. Keil, Brenda J. Buck, Dirk Goossens, Brett Mclaurin, Lacey Murphy, Mallory Leetham-Spencer, Yuanxin Teng, James E. Pollard, Russell Gerads, Jamie C. Dewitt

Geoscience Faculty Research

Systemic health effects from exposure to a complex natural dust containing heavy metals from the Nellis Dunes Recreation Area (NDRA) near Las Vegas, NV, were evaluated. Several toxicological parameters were examined following lung exposure to emissive dust from three geologic sediment types heavily used for recreational off-road activities: yellow sand very rich in arsenic (termed CBN 5); a shallow cover of loose dune sand overlying a gravelly subsoil bordering dune fields (termed CBN 6); and brown claystone and siltstone (termed CBN 7). Adult female B6C3F1 mice were exposed by oropharyngeal administration to these three types of geogenic dusts at 0.01–100 …

Crop Rotations That Integrate Pasture Are Less Vulnerable To Nitrogen Leaching Due To Changes In Microbial Community Composition And Reduced Nitrification, Jeth Gv Walkup

Graduate Theses, Dissertations, and Problem Reports (ETD)

Agroecosystems occupy a large portion of the United States and receive a disproportionally high amount of terrestrial nitrogen inputs. As application rates increase, nitrogen use efficiency declines leading to higher rates of nitrogen loss. To develop sustainable agricultural practices, the long-term effects of management practices on the soil microorganisms that perform central roles in the transformation of nitrogen need to be studied. To study the long-term impacts of compost application and pasture integration in crop rotation, samples were collected from the existing 19-year-old Organic Crop Livestock Field (OCLF) experiment on the WVU organic research farm. The OCLF experiment includes a …

Impacts Of Trace Metal Pollution, Urbanization And Aquifer Recharge On Spokane River Macroinvertebrates, Carolyn K. Connelly

EWU Masters Thesis Collection

This study analyzed macroinvertebrate communities of the Spokane River to determine: 1) accumulation of toxic trace metals in macroinvertebrate tissue, 2) effects of aquifer recharge, and 3) impact of urbanization. I predicted that aquifer inflows of cold, clean water would mitigate effects of pollution, and that reaches above the City of Spokane would be less negatively affected by urbanization; both were unsupported. I also predicted that effects of toxic trace metals would decrease within distance downstream; this prediction was supported. I sampled 12 riffles of the Spokane River between the Washington/Idaho border and Riverside State Park in late summer 2010. …