Structure-Function Analysis Of Nel, A Thrombospondin-1-Like Glycoprotein Involved In Neural Development And Functions, 2012 Valparaiso University
Structure-Function Analysis Of Nel, A Thrombospondin-1-Like Glycoprotein Involved In Neural Development And Functions, Masaru Nakamoto
Biology Faculty Publications
Nel (neural epidermal growth factor (EGF)-like molecule) is a multimeric, multimodular extracellular glycoprotein with heparin-binding activity and structural similarities to thrombospondin-1. Nel is predominantly expressed in the nervous system and has been implicated in neuronal proliferation and differentiation, retinal axon guidance, synaptic functions, and spatial learning. The Nel protein contains an N-terminal thrombospondin-1 (TSP-N) domain, five cysteine-rich domains, and six EGF-like domains. However, little is known about the functions of specific domains of the Nel protein. In this study, we have performed structure-function analysis of Nel, by using a series of expression constructs for different regions of the Nel protein. …
Standardised Classification Of Pre-Release Development In Male-Brooding Pipefish, Seahorses, And Seadragons (Family Syngnathidae), 2012 University of Zurich
Standardised Classification Of Pre-Release Development In Male-Brooding Pipefish, Seahorses, And Seadragons (Family Syngnathidae), Stefan Sommer, Camilla M. Whittington, Anthony B. Wilson
Publications and Research
Background: Members of the family Syngnathidae share a unique reproductive mode termed male pregnancy. Males carry eggs in specialised brooding structures for several weeks and release free-swimming offspring. Here we describe a systematic investigation of pre-release development in syngnathid fishes, reviewing available data for 17 species distributed across the family. This work is complemented by in-depth examinations of the straight-nosed pipefish Nerophis ophidion, the black-striped pipefish Syngnathus abaster, and the potbellied seahorse Hippocampus abdominalis.
Results: We propose a standardised classification of early syngnathid development that extends from the activation of the egg to the release of newborn. The classification consists …
Hyperosmotic Stress Enzyme Signaling Modulates Oct4, Nanog, And Rex1 Expression And Induces Prioritized Differentiation Of Murine Embryonic Stem Cells, 2012 Wayne State University
Hyperosmotic Stress Enzyme Signaling Modulates Oct4, Nanog, And Rex1 Expression And Induces Prioritized Differentiation Of Murine Embryonic Stem Cells, Jill A. Slater
Wayne State University Dissertations
HYPEROSMOTIC STRESS ENZYME SIGNALING MODULATES OCT4, NANOG,
AND REX1 EXPRESSION AND INDUCES PRIORITIZED DIFFERENTIATION OF
MURINE EMBRYONIC STEM CELLS
by
JILL SLATER
MAY 2013
Advisor: Daniel Rappolee, Ph.D.
Major: Physiology
Degree: Doctor of Philosophy
Transcription factor expression and therefore lineage identity in the periimplantation
embryo and its stem cells may be influenced by extracellular stresses,
potentially affecting pregnancy outcome. Cellular stress forces cells to suppress some
normal activities (such as protein synthesis and cell proliferation) in order to repair
stress-damaged macromolecules and restore homeostasis. Therefore, any new
activities that embryonic cells initiate while concurrently funding the demands of the
stress …
Pax6 Is Required For The Development Of The Lateral Procephalon In Tribolium, 2012 Wayne State University
Pax6 Is Required For The Development Of The Lateral Procephalon In Tribolium, Qing Luan
Wayne State University Theses
In Tribolium, combinatorial knockdown of the Pax6 orthologs eyeless (ey) and twin of eyeless (toy) affects the peripheral visual system but also other areas of the dorsal larval head capsule. To elucidate the role of Pax6 genes during Tribolium embryonic head development in detail, we performed an extensive analysis of cuticle
elements, brain anatomy, embryonic head morphogenesis and developmental marker gene expression. Our results reveal that Pax6 is required for the development of a large contiguous area of the lateral anterior head, morphologically addressed as the embryonic head lobes, which encompass the neuroectodermal precursor tissues of the visual system, parts …
Hslic Fall 2012 Scholarship Committee Report, 2012 The Jackson Laboratory
Hslic Fall 2012 Scholarship Committee Report, Ann Jordan
Ann Jordan
No abstract provided.
The Evolved Apprentice. How Evolution Made Humans Unique, 2012 MACCS Sydney, University of Edinburgh
The Evolved Apprentice. How Evolution Made Humans Unique, Mirko Farina
Mirko Farina
No abstract provided.
Do Ssds Extend The Conscious Mind?, 2012 University of Amsterdam
Do Ssds Extend The Conscious Mind?, Mirko Farina, Julian Kiverstein
Mirko Farina
Is the brain the biological substrate of consciousness? Most naturalistic philosophers of mind have supposed that the answer must obviously be «yes » to this question. However, a growing number of philosophers working in 4e (embodied, embedded, extended, enactive) cognitive science have begun to challenge this assumption, arguing instead that consciousness supervenes on the whole embodied animal in dynamic interaction with the environment. We call views that share this claim dynamic sensorimotor theories of consciousness (DSM). Clark (2009), a founder and leading proponent of the hypothesis of the extended mind, demurs, arguing that as matter of fact the biology of …
Basic Concepts In Understanding Recovery Of Function In Vestibular Reflex Networks During Vestibular Compensation, 2012 George Washington University
Basic Concepts In Understanding Recovery Of Function In Vestibular Reflex Networks During Vestibular Compensation, Kenna D. Peusner, Mei Shao, Rebecca Reddaway, June C. Hirsch
Anatomy and Regenerative Biology Faculty Publications
Unilateral peripheral vestibular lesions produce a syndrome of oculomotor and postural deficits with the symptoms at rest, the static symptoms, partially or completely normalizing shortly after the lesion due to a process known as vestibular compensation. The symptoms are thought to result from changes in the activity of vestibular sensorimotor reflexes. Since the vestibular nuclei must be intact for recovery to occur, many investigations have focused on studying these neurons after lesions. At present, the neuronal plasticity underlying early recovery from the static symptoms is not fully understood. Here we propose that knowledge of the reflex identity and input–output connections …
Elucidating The Role Of Bone Morphogenetic Proteins And B-Lymphocyte Maturation Protein 1 During Primordial Germ Cell Specification, 2012 California State University, San Bernardino
Elucidating The Role Of Bone Morphogenetic Proteins And B-Lymphocyte Maturation Protein 1 During Primordial Germ Cell Specification, Laughing Bear Torrez Dulgeroff
Theses Digitization Project
This study will present an optimized protocol to induce and isolate primordial germ cells (PGC) from hESC and hiPSC. Human embrionic stem cells (hESC and induced pluripotent stem cells (iPSC) provide a model for studying paradigms of early development.
Limb Bone Geometry And Skeletal Physiology In Mice Bred For High Levels Of Activity, 2012 California State University, San Bernardino
Limb Bone Geometry And Skeletal Physiology In Mice Bred For High Levels Of Activity, Brittney Racheal Coats
Theses Digitization Project
The studies presented here evaluate the effects of exercise and genetics on biomechanically important traits and the skeletal system's role in maintaining proper metabolic health. The goal of the project presented here is to further our understanding of the leptin-bone-insulin pathway and relevant changes that may occur in this pathway as a result of exercise. Cross-sectional analyses of HR mice and control mice allowed or denied wheel access for 10 weeks, revealed significant differences in biomechanical properties.
Determining The Role Of Wnt Signaling In Zebrafish Oocyte Maturation Through Examination Of Β-Catenin And Dishevelled Mrna Concentrations, 2012 University of Puget Sound
Determining The Role Of Wnt Signaling In Zebrafish Oocyte Maturation Through Examination Of Β-Catenin And Dishevelled Mrna Concentrations, Nathan Pincus
Summer Research
During oocyte maturation, the oocyte progresses from prophase I to metaphase II of meiosis, and a multitude of other cellular changes occur1. Wnt singaling pathways are known to regulate gene expression, cell behavior, cell adhesion, and cell polarity, as well as play an essential role in embryonic development. Because of this, I am examining the role of Wnt signaling pathways in the earlier process of oocyte maturation, specifically by looking at two Wnt signaling pathway components: β-catenin (ctnnb1) and Dishevelled (dvl2). β-catenin is a an interesting protein to study because it plays a dual role as both a cell adhesion …
Elucidating The Effects Of Tobacco Products On Skeletal Development, 2012 California State University, San Bernardino
Elucidating The Effects Of Tobacco Products On Skeletal Development, Nicole Renee Sparks
Theses Digitization Project
The overall goal of this thesis project is to characterize the molecular mechanisms of tobacco product induced skeletal teratogenicity.
Comparative Developmental Transcriptomics Of Echinoderms, 2012 University of South Florida
Comparative Developmental Transcriptomics Of Echinoderms, Roy Vaughn
USF Tampa Graduate Theses and Dissertations
The gastrula stage represents the point in development at which the three primary germ layers diverge. At this point the gene regulatory networks that specify the germ layers are established and the genes that define the differentiated states of the tissues have begun to be activated. These networks have been well characterized in sea urchins, but not in other echinoderms. Embryos of the brittle star Ophiocoma wendtii share a number of developmental features with sea urchin embryos, including the ingression of mesenchyme cells that give rise to an embryonic skeleton. Notable differences are that no micromeres are formed during cleavage …
Tgfßriib Mutations Trigger Aortic Aneurysm Pathogenesis By Altering Transforming Growth Factor Ss2 Signal Transduction, 2012 Philadelphia College of Osteopathic Medicine
Tgfßriib Mutations Trigger Aortic Aneurysm Pathogenesis By Altering Transforming Growth Factor Ss2 Signal Transduction, Katherine J. Bee, David C. Wilkes, Richard B. Devereux, Craig T. Basson, Cathy J. Hatcher
PCOM Scholarly Papers
Background-Thoracic aortic aneurysm (TAA) is a common progressive disorder involving gradual dilation of the ascending and/or descending thoracic aorta that eventually leads to dissection or rupture. Nonsydromic TAA can occur as a genetically triggered, familial disorder that is usually transmitted in a monogenic autosomal dominant fashion and is known as familial TAA. Genetic analyses of families affected with TAA have identified several chromosomal loci, and further mapping of familial TAA genes has highlighted disease-causing mutations in at least 4 genes: myosin heavy chain 11 (MYH11), a-smooth muscle actin (ACTA2), and transforming growth factor ß receptors I and II (TGFßRI and …
The Effects Of Hypoxia And Temperature On Developing Embryos Of The Annual Killifish Austrofundulus Limnaeus, 2012 Portland State University
The Effects Of Hypoxia And Temperature On Developing Embryos Of The Annual Killifish Austrofundulus Limnaeus, Skye N. Anderson
Dissertations and Theses
Little is known about the physiology or biochemistry of hypoxia (reduced levels of oxygen) tolerance during development in vertebrate embryos. In most species, relatively brief bouts of severe hypoxia are lethal or teratogenic. An exception to such hypoxia intolerance is the annual killifish Austrofundulus limnaeus, in which populations persist in hypoxic environments. This species inhabits seasonal ponds in Venezuela, surviving through the dry season in the form of diapausing embryos. Embedded in the pond sediment, embryos of A. limnaeus are routinely exposed to hypoxia and anoxia (lack of oxygen) as part of their normal development. Here, we exposed embryos to …
Systematics Of Leptopelis (Anura: Arthroleptidae) From The Itombwe Plateau, Eastern Democratic Republic Of The Congo, 2012 University of Texas at El Paso
Systematics Of Leptopelis (Anura: Arthroleptidae) From The Itombwe Plateau, Eastern Democratic Republic Of The Congo, Francisco Portillo
Open Access Theses & Dissertations
Leptopelis, a genus of Central African treefrogs, includes 51 species that live in tropical forests and savannas. Currently, only two species of Leptopelis are known from the poorly explored Itombwe Plateau in eastern Democratic Republic of the Congo (DRC). Itombwe is renowned among conservationists for its rich and endemic amphibian fauna, including: Xenopus itombwensis, Chrysobatrachus cupreonitens, Laurentophryne parkeri, Hyperolius leleupi and at least three species of Arthroleptis. Evolutionary relationships of Itombwe Leptopelis were examined by sequencing two mitochondrial genes (16S: 557 bp [base pairs], cyt b: 620 bp) and one nuclear gene (RAG1: 761 bp). Results recovered strong support for …
Age-Related Changes In The Neural Correlates Of Interval Timing, 2012 College of William & Mary - Arts & Sciences
Age-Related Changes In The Neural Correlates Of Interval Timing, Cutter Augustus. Lindbergh
Dissertations, Theses, and Masters Projects
No abstract provided.
A Proposal To Test The Effects Of Factor Ecat1 On Pluripotency, From Reprogramming To Differentiation Of Human Somatic Cells, 2012 Scripps College
A Proposal To Test The Effects Of Factor Ecat1 On Pluripotency, From Reprogramming To Differentiation Of Human Somatic Cells, Vritti R. Goel
CMC Senior Theses
The field of stem cell research has been growing more because of the interest in using stem cells to cure diseases and heal injuries. Human embryonic stem cells, because of the controversy surrounding them—and subsequently the difficulties in acquiring samples of the existing aging cell lines—can only be used in limited capacities. While the development of induced pluripotent stem cells in the last decade has allowed the field to progress closer to medical treatments, the low efficiency of reprogramming a somatic cell to a pluripotent state, and the vast molecular and genomic differences between human embryonic stem cells and human …
Metabolic Support Of Anaerobiosis In Embryos Of The Annual Killifish Austrofundulus Limnaeus, 2012 Portland State University
Metabolic Support Of Anaerobiosis In Embryos Of The Annual Killifish Austrofundulus Limnaeus, Andrew Mccracken
Dissertations and Theses
Embryos of the annual killifish Austrofundulus limnaeus display a remarkable tolerance to anoxia during development, most notably during embryonic diapause. Little is known about the metabolic or enzymatic changes that accompany this state of anoxia tolerance. This study examined the metabolic changes associated with exposure to anoxia by measuring the activity of the enzyme phosphoenolpyruvate carboxykinase (PEPCK), and by profiling the concentration of 31 metabolites ranging from amino acids to citric cycle intermediates at 4 different developmental stages, diapause 2 (DII), 4 days post diapause (dpd), 12 and 22 dpd. Embryos of A. limnaeus showed stage specific changes in concentrations …
The Role Of An Rna Binding Protein Hnrnp K During Axon Development And Regeneration In Xenopus Laevis, 2012 University at Albany, State University of New York
The Role Of An Rna Binding Protein Hnrnp K During Axon Development And Regeneration In Xenopus Laevis, Yuanyuan Liu
Legacy Theses & Dissertations (2009 - 2024)
Coordinated synthesis and assembly of the cytoskeletal network contribute significantly to morphological changes during axon outgrowth. Previous studies demonstrated that heterogeneous ribonucleoprotein K (hnRNP K), an RNA binding protein, binds to the 3'-untranslated regions of all neurofilament triplet subunits, the most abundant components of the axonal cytoskeleton. These findings raised the hypothesis that hnRNP K post-transcriptionally mediates the coordinated expression of axonal cytoskeletal components. In my thesis, I test this hypothesis during both axonal development and regeneration.