Delayed Expression Of Cell Cycle Proteins Contributes To Astroglial Scar Formation And Chronic Inflammation After Rat Spinal Cord Contusion, 2012 University of Maryland - Baltimore
Delayed Expression Of Cell Cycle Proteins Contributes To Astroglial Scar Formation And Chronic Inflammation After Rat Spinal Cord Contusion, Junfang Wu, Ahdeah Pajoohesh-Ganji, Bogdan A. Stoica, Michael Dinizo, Kelsey Guanciale, Alan I. Faden
Anatomy and Regenerative Biology Faculty Publications
Background
Traumatic spinal cord injury (SCI) induces secondary tissue damage that is associated with astrogliosis and inflammation. We previously reported that acute upregulation of a cluster of cell-cycle-related genes contributes to post-mitotic cell death and secondary damage after SCI. However, it remains unclear whether cell cycle activation continues more chronically and contributes to more delayed glial change. Here we examined expression of cell cycle-related proteins up to 4 months following SCI, as well as the effects of the selective cyclin-dependent kinase (CDKs) inhibitor CR8, on astrogliosis and microglial activation in a rat SCI contusion model.
Methods
Adult male rats were …
Uncovering Dual Roles For Perk Signaling During Experimentally Induced Pancreatitis, 2012 The University of Western Ontario
Uncovering Dual Roles For Perk Signaling During Experimentally Induced Pancreatitis, Elena Fazio
Electronic Thesis and Dissertation Repository
Pancreatitis is characterized by inappropriate activation of digestive enzyme
precursors, or zymogens, local and systemic inflammation, dysregulation of
cellular calcium (Ca2+), and induction of the unfolded protein response (UPR).
The UPR consists of three distinct pathways all of which are activated during
pancreatitis. However, the molecular roles of each remain unclear. The
protein kinase RNA (PKR)-like ER kinase (PERK) pathway reduces general
protein translation by phosphorylating eIF2!, and is activated within minutes
of initiating pancreatic damage. Microarray analysis carried out by our lab
revealed robust upregulation of the PERK pathways members Activating
Transcription Factor (ATF) 3 and stanniocalcin (STC) 2. …
The Role Of Nodal In The Regulation Of Bi-Potential Trophoblast Progenitor Cells, 2012 The University of Western Ontario
The Role Of Nodal In The Regulation Of Bi-Potential Trophoblast Progenitor Cells, Alia Cloutier-Bosworth
Electronic Thesis and Dissertation Repository
The human placenta develops from highly proliferative and phenotypically plastic cells called trophoblasts. Bi-potential trophoblast stem cells differentiate into the villous pathway to form the syncytiotrophoblast layer and the extravillous trophoblast (EVT). The HTR-8/SVneo cell line is widely used to study trophoblast biology. These cells variably express villous-specific or EVT-specific genes depending on conditions. Such phenotypic plasticity is indicative of a bi-potential cytotrophoblast progenitor. Preliminary work has shown that similar to progenitors in situ, a subpopulation of HTR-8/SVneo cells expresses a6b4 integrin. This a6b4high subset exhibits enhanced clonogenicity and differentiation capacity. This cell line also expresses Nodal, a …
Placental Insufficiency Resulting In Fetal Growth Restriction Alters Synaptic Development And Neuronal Myelination In Guinea Pigs At Term, 2012 The University of Western Ontario
Placental Insufficiency Resulting In Fetal Growth Restriction Alters Synaptic Development And Neuronal Myelination In Guinea Pigs At Term, Karolina Piorkowska
Electronic Thesis and Dissertation Repository
Aberrant neuronal connectivity in utero may underlie the association between fetal growth restriction (FGR) and increased risk for later cognitive disorders and encephalopathy. This study examines changes in synaptic development and myelination focussing on the hippocampus using a guinea pig model of placental insufficiency. Placental insufficiency was induced at mid-gestation by uterine artery ligation or cauterization which produced fetuses with a range of body weight and proportion at term. Synaptic markers, synaptophysin and synaptopodin, were decreased in FGR animals suggesting fewer synapses were formed and furthermore that fewer synapses matured with symmetrical growth restriction when compared to appropriate for gestational …
Role Of Progesterone Receptors In Neonatal Ovary Development, 2012 Syracuse University
Role Of Progesterone Receptors In Neonatal Ovary Development, Marta N. Dzyadyk
Honors Capstone Projects - All
In female mammals, proper oocyte development is a vital prerequisite for future gamete viability and fertility. This development of oocytes, known as oogenesis, begins with the migration of primordial germ cells to the genital ridge of the early embryo, where multiple rounds of mitotic division occur without complete cytokinesis. The result is temporary cyst morphology. Cyst breakdown is a crucial process in the next developmental stage, resulting in formation of the single oocytes which will grow in follicles surrounded by granulosa cells and eventually develop into eggs. These aspects of embryogenesis are conserved across multiple species, including Drosophila, mice, and …
Analyzing The Roles Of Scl And Gata3 In Zebrafish Spinal Cord Interneuron Specification And Function, 2012 Syracuse University
Analyzing The Roles Of Scl And Gata3 In Zebrafish Spinal Cord Interneuron Specification And Function, Kadiah Oyah Kamara
Honors Capstone Projects - All
My project focuses on the V2 cells of the zebrafish spinal cord. The V2 cells are an unusual class of spinal cells because they all originate from molecularly indistinguishable p2 progenitor cells in the spinal cord. However, as these cells become post-mitotic and differentiate, they start to express different transcription factor genes that allow them to initially develop into two sets of molecularly distinct cells. As differentiation continues, at least one more class of molecularly distinct cells develops. Just as in other vertebrates, in zebrafish the V2 cells differentiate into at least two functionally distinct classes of cells, specifically, the …
Specification Of The Neural Crest-Derived Pharyngeal Mesenchyme: The Role Of Pharyngeal Endoderm, Fgf And Bmp Signaling In The Establishment Of Prechondrogenic Identity And Initiation Of Chondrogenesis., 2012 Clemson University
Specification Of The Neural Crest-Derived Pharyngeal Mesenchyme: The Role Of Pharyngeal Endoderm, Fgf And Bmp Signaling In The Establishment Of Prechondrogenic Identity And Initiation Of Chondrogenesis., Megha Kumar
All Dissertations
This study investigates the role of pharyngeal endoderm in the specification of prechondrogenic identity and initiation of chondrogenesis in the proximal mesenchyme of the second pharyngeal arch. The results show that pharyngeal endoderm is sufficient but not necessary for specification of prechondrogenic identity in the cranial neural crest derived arch mesenchyme. A number of Fgf genes - Fgf3, 4, 8 and 19 are expressed in the pharyngeal endoderm. Further, FGF signaling is sufficient and required for the specification of prechondrogenic identity, marked by Sox9 expression. However, FGF signaling is unable to maintain the Sox9 expression and initiate the chondrogenic program. …
Mass Spectrometry-Based Proteomics Analysis Of The Matrix Microenvironment In Pluripotent Stem Cell Culture, 2012 The University of Western Ontario
Mass Spectrometry-Based Proteomics Analysis Of The Matrix Microenvironment In Pluripotent Stem Cell Culture, Christopher Hughes
Electronic Thesis and Dissertation Repository
The stem cell microenvironment contains soluble factors, support cells, and components of the extracellular matrix (ECM) that combine to effect cellular behavior. Mass spectrometry based proteomics offers the opportunity to directly assay components of extracellular microenvironments, thereby providing a sensitive means for obtaining insight into the stem cell niche. In this study we present the generation and analysis of human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) matrix microenvironments using an MS-based proteomics approach.
One of the primary limitations in the proteomics analysis of hESCs and hiPSCs is the reproducible generation of sufficient cell numbers amenable …
Genetic Identification Of Development Pathways Regulated By Conserved Micrornas In Caenorhabditis Elegans, 2012 Marquette University
Genetic Identification Of Development Pathways Regulated By Conserved Micrornas In Caenorhabditis Elegans, John L. Brenner
Dissertations (1934 -)
microRNAs (miRNAs) are approximately 22 nucleotide non-coding RNAs that function to repress genes by binding to complementary sites in target mRNAs and play critical roles in development and disease. It is predicted that more than 60% of human genes are regulated by miRNAs, however, little is known about the individual functions of miRNAs. I used the nematode worm, Caenorhabditis elegans, as a model to identify developmental processes and pathways regulated by conserved miRNAs. Genetic examination of miRNA function is hindered by lack of obvious phenotypes attributed to loss of individual miRNA genes. Phenotypes attributable to loss of individual miRNA genes …
Flatbed Scanner Report - Optical Density Dynamic Range, 2012 M.D. Anderson Cancer Center
Flatbed Scanner Report - Optical Density Dynamic Range, George Mcnamara
George McNamara
George McNamara (now at University of Miami) report for Hua Yu and Richard Jove, City of Hope National Medical Center, on optical density dynamic range of several flatbed scanners.
The Developmental And Adaptive Role Of Mitogen Activated Protein Kinase Pathways During Preimplantation Development, 2012 The University of Western Ontario
The Developmental And Adaptive Role Of Mitogen Activated Protein Kinase Pathways During Preimplantation Development, Christine E. Bell
Electronic Thesis and Dissertation Repository
The preimplantation period of development represents the highest interval of embryonic loss throughout pregnancy. It is therefore imperative that we elucidate the mechanisms involved in regulating preimplantation embryonic responses to stress and that govern development. The MAPK pathways are involved in both responding to environmental stress and regulation of development throughout embryogenesis, and are therefore good candidates to study the mechanisms involved in preimplantation embryonic adaptation to stress and development. The preimplantation embryo culminates in the development of a fluid filled structure called the blastocyst. It is at this stage the first differentiation events occur and the trophectoderm (TE), which …
Forisome Performance In Artificial Sieve Tubes, 2012 Indiana University - Purdue University Fort Wayne
Forisome Performance In Artificial Sieve Tubes, Michael Knoblauch, Mike Stubenrauch, Aart J.E. Van Bel, Winfried S. Peters
Winfried S. Peters
Introduction To Nanoscopy Nano-Talk, 2012 M.D. Anderson Cancer Center
Introduction To Nanoscopy Nano-Talk, George Mcnamara
George McNamara
T7-1 is the designation for the LMRG Nanoscopy session at ABRF in Orlando, FL, on March 20, 2012. The PDF file here is a draft of my presentation.
May not be very helpful since (1) would probably help to know what is in my head and each slide will [hopefully] prompt me to say, and (2) 10 minute talk so I am going to push the "next slide" button after saying very little.
__________________
Publisher statement:
The T7-1 Introduction to Nanoscopy Nano Talk is copyrighted (c) George McNamara, 2012. Except for (1) screenshots from research articles (which are copyrighted by …
Pubspectra - Open Data Access Fluorescence Spectra, 2012 M.D. Anderson Cancer Center
Pubspectra - Open Data Access Fluorescence Spectra, George Mcnamara
George McNamara
The Internet is enabling greater access to spectral imaging publications, spectral graphs, and data than that was available a generation ago. The spectral imaging systems discussed in this issue of Cytometry work because reagent and hardware spectra are reproducible, reusable, and provide input to spectral unmixing and spectral components recognition algorithms. These spectra need to be readily available in order to determine what to purchase, how to use it, and what the output means. We refer to several commercially sponsored and academic spectral web sites and discuss our spectral graphing and data sites. Sites include fluorescent dye graph servers from …
Mcnamara 2011 Feature Extraction (Image Analysis), 2012 M.D. Anderson Cancer Center
Mcnamara 2011 Feature Extraction (Image Analysis), George Mcnamara
George McNamara
Feature Extraction presentation and movies in a ZIP file from a presentation I gave at ISAC 2011 in Baltomore, Md.
Feature extraction is one phrase for image analysis.
Rogers Pmn Movie - Background Information, 2012 M.D. Anderson Cancer Center
Rogers Pmn Movie - Background Information, George Mcnamara
George McNamara
Please see
http://mdc.custhelp.com/app/answers/detail/a_id/18689/~/metamatters-newsletters
for my series of MetaMorph MetaMatters articles in volume 2, numbers 3 through 6, on the Rogers PMN Panorama data set.
http://mdc.custhelp.com/app/answers/detail/a_id/18689/~/metamatters-newsletters
Brief summary (for more, see the Word doc)
Expression Of 14-3-3 Protein Isoforms In Mouse Oocytes, Eggs And Ovarian Follicular Development, 2012 Kent State University - Kent Campus
Expression Of 14-3-3 Protein Isoforms In Mouse Oocytes, Eggs And Ovarian Follicular Development, Santanu De, Jennifer Marcinkiewicz, Srinivasan Vijayaraghavan, Douglas Kline
Biology Faculty Articles
Background
The 14-3-3 (YWHA) proteins are a highly conserved, ubiquitously expressed family of proteins. Seven mammalian isoforms of 14-3-3 are known (β, γ, ε, ζ, η, τ and, σ). These proteins associate with many intracellular proteins involved in a variety of cellular processes including regulation of the cell cycle, metabolism and protein trafficking. We are particularly interested in the role of 14-3-3 in meiosis in mammalian eggs and the role 14-3-3 proteins may play in ovarian function. Therefore, we examined the expression of 14-3-3 proteins in mouse oocyte and egg extracts by Western blotting after polyacrylamide gel electrophoresis, viewed fixed …
Rhythmic Growth And Vascular Development In Brachypodium Distachyon, 2012 University of Massachusetts Amherst
Rhythmic Growth And Vascular Development In Brachypodium Distachyon, Dominick A. Matos
Masters Theses 1911 - February 2014
Plants reduce inorganic carbon to synthesize biomass that is comprised of mostly polysaccharides and lignin. Growth is intricately regulated by external cues such as light, temperature, and water availability and internal cues including those generated by the circadian clock. While many aspects of polymer biosynthesis are known, their regulation and distribution within the stem are poorly understood. Plant biomass is perhaps the most abundant organic substance on Earth and can be used as feedstock for energy production. Various grass species are under development as energy crops yet several of their attributes make them challenging research subjects. Brachypodium distachyon has emerged …
The Methuselah Family Of G Protein Coupled Receptors, 2012 Wayne State University
The Methuselah Family Of G Protein Coupled Receptors, Meghna V. Patel
Wayne State University Dissertations
G protein-coupled receptors (GPCRs) are one of the largest class of transmembrane signaling proteins that regulate essential developmental and physiological processes in a cell. GPCR success is illustrated by their abundance across both invertebrate and vertebrate genomes. Phylogenetic analyses show that GPCR families have undergone a lot of gene gain and loss during insect evolution. In Drosophila melanogaster, the fifteen Methuselah/Methuselah-like (Mth/Mthl) genes are in fact an insect specific family of GPCRs. In our study, we conducted a phylogenetic analysis using receptor sequences from five Drosophila species and two related insects, including Tribolium and Anopheles to examine the evolution of …
Rhodococcus Equi Infection And Interferon-Gamma Regulation In Foals, 2012 University of Kentucky
Rhodococcus Equi Infection And Interferon-Gamma Regulation In Foals, Lingshuang Sun
Theses and Dissertations--Veterinary Science
Rhodococcus equi (R. equi) is one of the most serious causes of pneumonia in young foals. The clinical disease is of great concern to breeding farms worldwide due to the impact of mortality on economic losses. While adult horses are resistant to R. equi, foals exhibit a distinct age-associated susceptibility. The mechanism underlying this susceptibility in foals is not well understood. Interferon-gamma (IFNg) plays an important role in the clearance of R. equi, but its expression is impaired in neonatal foals. Moreover, the regulation of this age-related IFNg expression in foals remains unknown. In humans, IFNg …