Role Of Human Cytomegalovirus In Breast Cancer,
2012
The University of San Francisco
Role Of Human Cytomegalovirus In Breast Cancer, Cendy Valle Oseguera, Juliet V. Spencer
Creative Activity and Research Day - CARD
Cancer results from unregulated cell growth that invades neighboring tissues. While there are many known risk factors for cancer, one area that remains largely unexplored is the impact of infectious disease. Human cytomegalovirus (HCMV) is highly prevalent in the population and has also been found in several types of tumors. We are investigating the possibility that a viral protein released from infected cells, cmvIL-10, promotes tumor metastasis by enhancing the invasive potential of breast cancer cells. These results will clarify the role of HCMV in breast cancer progression and could have broad implications for the diagnosis and treatment of cancer.
Analysis Of Her-2 Positive Breast Cancer Cell Lines,
2012
Department of Biological Sciences, University of Texas at El Paso
Analysis Of Her-2 Positive Breast Cancer Cell Lines, Natzidielly Lerma^, Paloma Valenzuela, Karla Parra, Eduardo Ramirez, Courtney L. Becerril, Irving Miramontes, Cynthia M. Rodriguez, Elizabeth Gamez, Giulio Francia*
COURI Symposium Abstracts, Spring 2012
No abstract provided.
Defeating Cytoplasmic Sequestration Of P53 In Human Breast Cancer Cells; Is Mortalin Involved?,
2012
University of New Hampshire - Main Campus
Defeating Cytoplasmic Sequestration Of P53 In Human Breast Cancer Cells; Is Mortalin Involved?, Sarah Yunes
Honors Theses and Capstones
Cytoplasmic sequestration of p53, possibly caused by p53 interacting with mortalin, can prevent p53 from functioning in DNA repair and apoptosis, causing aberrant growth. This project treated SKBR3 breast cancer cells with MKT-077, a dye that is a competitive binder to mortalin to see if it would result in the release of p53 from the cytoplasm and restoration of p53 function. Treatment resulted in partial translocation of a protein suspected to be p53 to the nucleus and apoptosis initiated at the mitochondria.
Inhibition Of Fatty Acid Synthase Attenuates Cd44-Associated Signaling And Reduces Metastasis In Colorectal Cancer,
2012
University of Kentucky
Inhibition Of Fatty Acid Synthase Attenuates Cd44-Associated Signaling And Reduces Metastasis In Colorectal Cancer, Yekaterina Y. Zaytseva, Piotr G. Rychahou, Pat Gulhati, Victoria Allison Elliott, William Conan Mustain, Kathleen O'Connor, Andrew J. Morris, Manjula Sunkara, Heidi L. Weiss, Eun Young Lee, B. Mark Evers
Markey Cancer Center Faculty Publications
Fatty acid synthase (FASN) and ATP-citrate lyase, key enzymes of de novo lipogenesis, are significantly upregulated and activated in many cancers and portend poor prognosis. Even though the role of lipogenesis in providing proliferative and survival advantages to cancer cells has been described, the impact of aberrant activation of lipogenic enzymes on cancer progression remains unknown. In this study, we found that elevated expression of FASN is associated with advanced stages of colorectal cancer (CRC) and liver metastasis, suggesting that it may play a role in progression of CRC to metastatic disease. Targeted inhibition of lipogenic enzymes abolished expression of …
Chip Sequencing Of Cyclin D1 Reveals A Transcriptional Role In Chromosomal Instability In Mice.,
2012
Kimmel Cancer Center, Thomas Jefferson University
Chip Sequencing Of Cyclin D1 Reveals A Transcriptional Role In Chromosomal Instability In Mice., Mathew C Casimiro, Marco Crosariol, Emanuele Loro, Adam Ertel, Zuoren Yu, William Dampier, Elizabeth A Saria, Alex Papanikolaou, Timothy J Stanek, Zhiping Li, Chenguang Wang, Paolo Fortina, Sankar Addya, Aydin Tozeren, Erik S Knudsen, Andrew Arnold, Richard G Pestell
Kimmel Cancer Center Faculty Papers
Chromosomal instability (CIN) in tumors is characterized by chromosomal abnormalities and an altered gene expression signature; however, the mechanism of CIN is poorly understood. CCND1 (which encodes cyclin D1) is overexpressed in human malignancies and has been shown to play a direct role in transcriptional regulation. Here, we used genome-wide ChIP sequencing and found that the DNA-bound form of cyclin D1 occupied the regulatory region of genes governing chromosomal integrity and mitochondrial biogenesis. Adding cyclin D1 back to Ccnd1-/- mouse embryonic fibroblasts resulted in CIN gene regulatory region occupancy by the DNA-bound form of cyclin D1 and induction of CIN …
Targeting The Cancer Cell Cycle By Cold Atmospheric Plasma,
2012
George Washington University
Targeting The Cancer Cell Cycle By Cold Atmospheric Plasma, Olga Volotskova, Teresa S. Hawley, Mary Ann Stepp, Michael Keidar
Anatomy and Regenerative Biology Faculty Publications
Cold atmospheric plasma (CAP), a technology based on quasi-neutral ionized gas at low temperatures, is currently being evaluated as a new highly selective alternative addition to existing cancer therapies. Here, we present a first attempt to identify the mechanism of CAP action. CAP induced a robust ~2-fold G2/M increase in two different types of cancer cells with different degrees of tumorigenicity. We hypothesize that the increased sensitivity of cancer cells to CAP treatment is caused by differences in the distribution of cancer cells and normal cells within the cell cycle. The expression of γH2A.X (pSer139), an oxidative stress reporter indicating …
Effective Non-Viral Delivery Of Sirna To Acute Myeloid Leukemia Cells With Lipid-Substituted Polyethylenimines,
2012
University of Alberta
Effective Non-Viral Delivery Of Sirna To Acute Myeloid Leukemia Cells With Lipid-Substituted Polyethylenimines, Breanne Landry
Pharmacy Faculty Articles and Research
Use of small interfering RNA (siRNA) is a promising approach for AML treatment as the siRNA molecule can be designed to specifically target proteins that contribute to aberrant cell proliferation in this disease. However, a clinical-relevant means of delivering siRNA molecules must be developed, as the cellular delivery of siRNA is problematic. Here, we report amphiphilic carriers combining a cationic polymer (2 kDa polyethyleneimine, PEI2) with lipophilic moieties to facilitate intracellular delivery of siRNA to AML cell lines. Complete binding of siRNA by the designed carriers was achieved at a polymer:siRNA ratio of ~0.5 and led to siRNA/polymer complexes of …
Chemotherapy: The Physiological Cost Of A Cure,
2012
Parkland College
Chemotherapy: The Physiological Cost Of A Cure, Megan Ellis
A with Honors Projects
This project focuses on the common long term side effects of cancer treatments, apart from cure. In addition to physiological function changes, it focuses on the chemical composition of chemotherapy drugs.
The Effects Of Cancer,
2012
Parkland College
The Effects Of Cancer, Mara Lapayne
A with Honors Projects
I chose to do a project related to cancer for my Painting 1 class. Using oil paint, I wanted to create a more symbolic representation of cancer. So I made a cloud-like, cancerous mass with streaks of chaotic color swirling around a central face. The face represents any person affected by cancer and has had to “shut down” their emotions in order to function. I wanted the skin texture to be almost metallic as if it is metal armor or robotic-like, to support the idea of “shutting down” one’s emotions. The blank expression also portrays this idea.
Genetics And Cancer,
2012
Parkland College
Genetics And Cancer, Sachin Puri
A with Honors Projects
Genes' effect in body and relationship with cancer. Role in cell cycle and angiogenesis.
Kshv Infection Of Endothelial Cells Manipulates Cxcr7-Mediated Signaling: Implications For Kaposi’S Sarcoma Progression And Intervention,
2012
Chapman University
Kshv Infection Of Endothelial Cells Manipulates Cxcr7-Mediated Signaling: Implications For Kaposi’S Sarcoma Progression And Intervention, Jennifer Totonchy, Lisa Clepper, Janet Douglas, Liron Pantanowitz, Klaus Fruh, Ashlee V. Moses
Pharmacy Faculty Articles and Research
"CXCR7 was recently characterized as an alternative receptor for the chemokine CXCL12/SDF-1, previously thought to bind and signal exclusively through CXCR4.We recently identified CXCR7 as a key cellular factor in the endothelial cell (EC) dysfunction associated with KSHV infection. CXCL12 signaling is critically associated with tumor growth, angiogenesis and metastasis in several diverse tumors and is one of the most studied chemokine/chemokine receptor interactions in cancer systems. The tumorigenic activity of the CXCL12 signaling axis offers an attractive target for therapeutic intervention in multiple cancers including Kaposi’s Sarcoma (KS). However, most of the research to date was based on the …
Cytoplasmic Sequestration Of The Tumor Suppressor P53 By A Heat Shock Protein 70 Family Member, Mortalin, In Human Colorectal Adenocarcinoma Cell Lines,
2012
West Chester University of Pennsylvania
Cytoplasmic Sequestration Of The Tumor Suppressor P53 By A Heat Shock Protein 70 Family Member, Mortalin, In Human Colorectal Adenocarcinoma Cell Lines, Erin E. Gestl, S. Anne Boettger
Biology Faculty Publications
No abstract provided.
Cancer And You,
2012
Parkland College
Cancer And You, Diah-Aldeen Judeh
A with Honors Projects
This project discusses ways a person can identify cancer and what to do if cancer is present.
The Linkage Between Transcription Control And Epigenetic Regulation: The Snail Story And Beyond,
2012
University of Kentucky
The Linkage Between Transcription Control And Epigenetic Regulation: The Snail Story And Beyond, Yiwei Lin
Theses and Dissertations--Pharmacology and Nutritional Sciences
Epigenetic deregulation contributes significantly to the development of multiple human diseases, including cancer. While great effort has been made to elucidate the underlying mechanism, our knowledge on epigenetic regulation is still fragmentary, an important gap being how the diverse epigenetic events coordinate to control gene transcription. In the first part of our study, we demonstrated an important link between Snail-mediated transcriptional control and epigenetic regulation during cancer development. Specifically, we found that the highly conserved SNAG domain of Snail sequentially and structurally mimics the N-terminal tail of histone H3, thereby functions as a molecular “hook”, or pseudo substrate, for recruiting …
Probing Molecular Mechanisms Of The Hsp90 Chaperone: Biophysical Modeling Identifies Key Regulators Of Functional Dynamics,
2012
University of Kansas - Main Campus
Probing Molecular Mechanisms Of The Hsp90 Chaperone: Biophysical Modeling Identifies Key Regulators Of Functional Dynamics, Anshuman Dixit, Gennady M. Verkhivker
Mathematics, Physics, and Computer Science Faculty Articles and Research
Deciphering functional mechanisms of the Hsp90 chaperone machinery is an important objective in cancer biology aiming to facilitate discovery of targeted anti-cancer therapies. Despite significant advances in understanding structure and function of molecular chaperones, organizing molecular principles that control the relationship between conformational diversity and functional mechanisms of the Hsp90 activity lack a sufficient quantitative characterization. We combined molecular dynamics simulations, principal component analysis, the energy landscape model and structure-functional analysis of Hsp90 regulatory interactions to systematically investigate functional dynamics of the molecular chaperone. This approach has identified a network of conserved regions common to the Hsp90 chaperones that could …
Analysis Of Human Tacc3 In Cellular Responses To Polycyclic Aromatic Hydrocarbons,
2012
Arkansas Tech University
Analysis Of Human Tacc3 In Cellular Responses To Polycyclic Aromatic Hydrocarbons, Ivan H. Still, B. Lauffart
Journal of the Arkansas Academy of Science
Very little is known about the etiology of ovarian cancer. However, studies have shown that occupational exposure to polycyclic aromatic hydrocarbons (PAHs) and tobacco smoking can increase ovarian cancer risk. Previously, we have determined that 100% of ovarian tumors show loss or aberrant subcellular localization of TACC3 (transforming acidic coiled coil 3) relative to the normal ovarian surface epithelium (OSE). In mice, a role for TACC3 in the cellular response to PAH has been defined. However, comparable studies for human TACC3 has not been performed. In this report, we show that specific knockdown of TACC3 in human ovarian cancer cells …
Simulating Molecular Mechanisms Of The Mdm2-Mediated Regulatory Interactions: A Conformational Selection Model Of The Mdm2 Lid Dynamics,
2012
Chapman University
Simulating Molecular Mechanisms Of The Mdm2-Mediated Regulatory Interactions: A Conformational Selection Model Of The Mdm2 Lid Dynamics, Gennady M. Verkhivker
Mathematics, Physics, and Computer Science Faculty Articles and Research
Diversity and complexity of MDM2 mechanisms govern its principal function as the cellular antagonist of the p53 tumor suppressor. Structural and biophysical studies have demonstrated that MDM2 binding could be regulated by the dynamics of a pseudo-substrate lid motif. However, these experiments and subsequent computational studies have produced conflicting mechanistic models of MDM2 function and dynamics. We propose a unifying conformational selection model that can reconcile experimental findings and reveal a fundamental role of the lid as a dynamic regulator of MDM2-mediated binding. In this work, structure, dynamics and energetics of apo-MDM2 are studied as a function of posttranslational modifications …
Cutaneous Papilloma And Squamous Cell Carcinoma Therapy Utilizing Nanosecond Pulsed Electric Fields (Nspef),
2012
Old Dominion University
Cutaneous Papilloma And Squamous Cell Carcinoma Therapy Utilizing Nanosecond Pulsed Electric Fields (Nspef), Dong Yin, Wangrong G. Yang, Jack Weissberg, Catherine B. Goff, Weikai Chen, Yoshio Kuwayama, Amanda Leiter, Hongtao Xing, Antonie Meixel, Daria Gaut, Fikret Kirkbir, David Sawcer, P. Thomas Vernier, Jonathan W. Said, Martin A. Gundersen, H. Phillip Koeffler
Bioelectrics Publications
Nanosecond pulsed electric fields (nsPEF) induce apoptotic pathways in human cancer cells. The potential therapeutic effective of nsPEF has been reported in cell lines and in xenograft animal tumor model. The present study investigated the ability of nsPEF to cause cancer cell death in vivo using carcinogen-induced animal tumor model, and the pulse duration of nsPEF was only 7 and 14 nano second (ns). An nsPEF generator as a prototype medical device was used in our studies, which is capable of delivering 7-30 nanosecond pulses at various programmable amplitudes and frequencies. Seven cutaneous squamous cell carcinoma cell lines and five …
Effect Of Chlorogenic Acid And Neochlorogenic Acid On Human Colon Cancer Cells,
2012
University of Arkansas, Fayetteville
Effect Of Chlorogenic Acid And Neochlorogenic Acid On Human Colon Cancer Cells, Taylor Thurow, Sun-Ok Lee
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
Prune consumption has been associated with a decreased risk of colon cancer; yet there has been confusion as to which chemical component(s) of the prune are responsible for its anticarcinogenic properties. Previous studies have evaluated chlorogenic acid as a chemotherapeutic agent; however, only a limited amount of studies have investigated neochlorogenic acid, the predominant phenolic compound found in the prune. The purpose of this study was to determine the effects that chlorogenic acid and neochlorogenic acid have as anticarcinogenics on the human adenocarcinoma, Caco-2 cell line. The anti-colon cancer effects of chlorogenic acid and neochlorogenic acid were analyzed by experiments …
Cdk1 And Plk1 Mediate A Clasp2 Phospho-Switch That Stabilizes Kinetochore–Microtubule Attachments,
2012
University of Porto
Cdk1 And Plk1 Mediate A Clasp2 Phospho-Switch That Stabilizes Kinetochore–Microtubule Attachments, Ana R. R. Maia, Zaira Garcia, Lilian Kabeche, Marin Barisic
Dartmouth Scholarship
Accurate chromosome segregation during mitosis relies on a dynamic kinetochore (KT)-microtubule (MT) interface that switches from a labile to a stable condition in response to correct MT attachments. This transition is essential to satisfy the spindle-assembly checkpoint (SAC) and couple MT-generated force with chromosome movements, but the underlying regulatory mechanism remains unclear. In this study, we show that during mitosis the MT- and KT-associated protein CLASP2 is progressively and distinctively phosphorylated by Cdk1 and Plk1 kinases, concomitant with the establishment of KT-MT attachments. CLASP2 S1234 was phosphorylated by Cdk1, which primed CLASP2 for association with Plk1. Plk1 recruitment to KTs …
