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Factors Affecting Cytoplasmic And Nuclear Structures Of Mouse Gametes And Embryos Using Immunocytochemistry Technique., Nooraain Hashim 2010 Universiti Malaya

Factors Affecting Cytoplasmic And Nuclear Structures Of Mouse Gametes And Embryos Using Immunocytochemistry Technique., Nooraain Hashim

Student Works (2010-2019)

Experiment 1 was conducted with the main objective to establish ICC technique for morphological analysis of the mouse oocytes and embryos in local laboratory. Several tests were conducted to achieve specific aims which included; a) to study efficacy of the available instruments and system for detecting fluorescence signals from mouse oocytes, b) to compare efficiency of improved methods to reveal oocyte morphology. Fluorescence images of the meiotic spindles, cortical granules and nuclear materials were compared between sample preparations methods. The results showed that the methods employed were reliable to be used for detecting the meiotic spindle, cortical granules and nuclear …


The Effects Of The Ground Reaction Force On The Muscle-Tendon Systems About Guinea Pig Joints, Theresa Schill, Kevin Mailland, David V. Lee 2010 University of Nevada, Las Vegas

The Effects Of The Ground Reaction Force On The Muscle-Tendon Systems About Guinea Pig Joints, Theresa Schill, Kevin Mailland, David V. Lee

Undergraduate Research Opportunities Program (UROP)

Each limb of the limb joints of a quadruped is used differently during locomotion, and it is necessary to recognize the mechanical role each plays. The effects of the ground reaction force on the ankle, knee, and hip joints of Hartley albino guinea pigs were studied in uphill, downhill, and level situations to determine the spring-like capacity of the muscle-tendon systems that act about the limb joints.


Impact Of Historical Climate Change On The Genetic Structure Of The Great Basin Pocket Mouse, Perognathus Parvus, Ashwitha Francis, Mallory E. Eckstut, Brett R. Riddle 2010 University of Nevada, Las Vegas

Impact Of Historical Climate Change On The Genetic Structure Of The Great Basin Pocket Mouse, Perognathus Parvus, Ashwitha Francis, Mallory E. Eckstut, Brett R. Riddle

Undergraduate Research Opportunities Program (UROP)

The dynamics of genetic patterns, such as genetic differentiation within and between populations and the geographic distribution of genetic lineages, are often influenced by historical events (such as climatic changes) that have substantially impacted regional biodiversity (the study of phylogeography; Avise, 20001). The magnitude of genetic differentiation among populations is often increased if suitable habitat is reduced, because there is restricted migration among populations. When migration is restricted, the populations may become genetically differentiated from conspecific populations and, over time, may become independent lineages that follow distinctive evolutionary trajectories (Stebbins, 19523; Nevo & Beiles, 19892; Thomas et al., 19984). In …


3d Micro-Mr And Micro-Ct Models For Determining In-Vivo Function In The Guinea Pig Knee Model Of Osteoarthritis (Oa), Kristoffer W. Dean 2010 University of Nevada, Las Vegas

3d Micro-Mr And Micro-Ct Models For Determining In-Vivo Function In The Guinea Pig Knee Model Of Osteoarthritis (Oa), Kristoffer W. Dean

Undergraduate Research Opportunities Program (UROP)

Osteoarthritis (OA) is a debilitating disease that affects an estimated 27 million adults in the United States. OA causes damage to cartilage in the joints resulting in bones that rub together. This creates pain, swelling, loss of motion of the joint, and bone spurs. As the number of adults above 65 grows (an estimated 72 million people by 2030) even more people will be at high risk for OA. It is the most common type of arthritis by far yet very little is known about it including relationships between joint structure and function. In 2004, the NIH created the Osteoarthritis …


Ubiquitin Dependent Proteolysis In The Frozen Wood Frog, Michael Ulrich, Ken Storey, Frank van Breukelen 2010 University of Nevada, Las Vegas

Ubiquitin Dependent Proteolysis In The Frozen Wood Frog, Michael Ulrich, Ken Storey, Frank Van Breukelen

Undergraduate Research Opportunities Program (UROP)

Wood frogs (Rana sylvatica) freeze during the winter. One might presume that homeostatic processes like protein synthesis and degradation cease during freezing. However, few data are available. In collaboration with Dr. Ken Storey (Carelton University, Canada), we are investigating the primary mode of proteolysis in wood frogs representing different points of the freeze-thaw cycle.


Environment-Induced Phenotypic Plasticity In The Teeth Of Hatchery And Wild Largemouth Bass, Micropterus Floridanus, Tamilselvi Selvaraj 2010 Western Kentucky University

Environment-Induced Phenotypic Plasticity In The Teeth Of Hatchery And Wild Largemouth Bass, Micropterus Floridanus, Tamilselvi Selvaraj

Masters Theses & Specialist Projects

Successful stock enhancement of hatchery-reared fish depends heavily on the release of individuals able to demonstrate strong survival skills. Overall survival of fishes is a reflection of a successful blend of physiology, anatomy, and behavior. With fishes being highly phenotypically plastic, the potential exists for all aspects of hatcheryreared fish to vary significantly from their wild counterparts while having potentially adverse effects on their survival after release. Previous analyses have demonstrated significant differences between the feeding behavior of hatchery-reared and wild caught largemouth bass (Micropterus floridanus) in the laboratory, as well as differences in the development of the …


Kentucky Warbler (Vol. 86, No. 3), Kentucky Library Research Collections 2010 Western Kentucky University

Kentucky Warbler (Vol. 86, No. 3), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.


Capuchins (Cebus Apella) Can Solve A Means-End Problem, Anna M. Yocom, Sarah T. Boysen 2010 The Ohio State University

Capuchins (Cebus Apella) Can Solve A Means-End Problem, Anna M. Yocom, Sarah T. Boysen

Sentience Collection

Three capuchin monkeys (Cebus apella) were tested on a 2-choice discrimination task designed to examine their knowledge of support, modeled after Hauser, Kralik, and Botto-Mahan’s (1999) experiments with tamarins. This task involved a choice between 2 pieces of cloth, including 1 with a food reward placed on its surface, and a second cloth with the food reward next to its surface. After reliably solving the basic problem, the capuchins were tested with various alternations of the original food reward and cloth. The capuchins were able to solve the initial task quickly, and generalize their knowledge to additional functional and nonfunctional …


The Impact Of Antioxidant Addition On Flavor Of Cheddar And Mozzarella Whey And Cheddar Whey Protein Concentrate, I. W. Liaw, H. Eshpari, P. S. Tong, M. A. Drake 2010 North Carolina State University at Raleigh

The Impact Of Antioxidant Addition On Flavor Of Cheddar And Mozzarella Whey And Cheddar Whey Protein Concentrate, I. W. Liaw, H. Eshpari, P. S. Tong, M. A. Drake

Dairy Science

Lipid oxidation products are primary contributors to whey ingredient off-flavors. The objectives of this study were to evaluate the impact of antioxidant addition in prevention of flavor deterioration of fluid whey and spray-dried whey protein. Cheddar and Mozzarella cheeses were manufactured in triplicate. Fresh whey was collected, pasteurized, and defatted by centrifugal separation. Subsequently, 0.05% (w/w) ascorbic acid or 0.5% (w/w) whey protein hydrolysate (WPH) were added to the pasteurized whey. A control with no antioxidant addition was also evaluated. Wheys were stored at 3 °C and evaluated after 0, 2, 4, 6, and 8 d. In a subsequent experiment, …


Protecting Inshore And Demersal Finfish Identification Of Critical Habitats For Juvenile Dhufish Workshop Report, Gabby E. Mitsopoulos, Brett W. Molony 2010 Department of Primary Industries and Regional Development, Western Australia

Protecting Inshore And Demersal Finfish Identification Of Critical Habitats For Juvenile Dhufish Workshop Report, Gabby E. Mitsopoulos, Brett W. Molony

Fisheries Research Reports

A Juvenile Dhufish Workshop was held at the Western Australian Fisheries and Marine Research Laboratories (WAFMRL) on 18th March 2010, which was the first component of a State Natural Resource Management (NRM) funded project entitled “Protecting Inshore and Demersal Finfish - Identification of Critical Habitats for Juvenile Dhufish”. The primary objective of the workshop was to provide a forum for discussion in order to compile all biological, ecological and anecdotal information on the early life history of dhufish, to create hypotheses of habitat requirements for juvenile dhufish and provide recommendations as to which sampling methods should be used to confirm …


Review Of The Exploitation Of Marine Resources Of The Australian Indian Ocean Territories: The Implications Of Biogeographic Isolation For Tropical Island Fisheries, Matthew Hourston 2010 Department of Primary Industries and Regional Development, Western Australia

Review Of The Exploitation Of Marine Resources Of The Australian Indian Ocean Territories: The Implications Of Biogeographic Isolation For Tropical Island Fisheries, Matthew Hourston

Fisheries Research Reports

The small islands of the Indian Ocean Territories are isolated reefs in an expanse of open ocean of abyssal depth. The majority of marine species that live on their reefs and lagoons have settled there from remote locations. Colonising individuals are presumed to have come to the island as the result of unusual weather and current conditions and form populations that are, necessarily, largely self-sustaining. The isolated populations that make up many of the fish and invertebrate stocks at the Cocos (Keeling) and Christmas Islands rely almost exclusively on larvae returning to their home reef to settle, grow and ultimately …


The Effects Of Growth Hormone In The Inner Ear Of Zebrafish (Danio Rerio) During Hair Cell Regeneration, Chia-Hui Lin 2010 Western Kentucky University

The Effects Of Growth Hormone In The Inner Ear Of Zebrafish (Danio Rerio) During Hair Cell Regeneration, Chia-Hui Lin

Masters Theses & Specialist Projects

Although deafness is a universal problem, effective treatments have remained elusive. In order to develop potential treatments, an overall understanding of the cellular process of auditory hair cell regeneration, which occurs in fish but not mammals, must be established. A previous microarray analysis and qRT-PCR validation of noise-exposed zebrafish showed that growth hormone (GH) was significantly upregulated during the process of auditory hair cell regeneration. Thus, GH may play an important role during hair cell regeneration. However, cellular effects of exogenous GH in the zebrafish auditory hair cell regeneration have not been examined after noise exposure. To understand the effect …


Age, Reproduction, Growth, Condition And Diet Of The Introduced Yellow Bass, Morone Mississippiensis, In Barren River Lake, Kentucky, Peter G. Zervas 2010 Western Kentucky University

Age, Reproduction, Growth, Condition And Diet Of The Introduced Yellow Bass, Morone Mississippiensis, In Barren River Lake, Kentucky, Peter G. Zervas

Masters Theses & Specialist Projects

Introduction of fish species to North American drainages has occurred for over 100 years. Introduced fish species have been documented to have adverse effects on both the environment and native species of the drainage into which they have been introduced. To better understand the effects that introduced species may have on a particular drainage, it is essential to understand aspects of the introduced species’ life history. The objectives of the current study is to quantify the age, reproduction, growth, condition and diet of the yellow bass, Morone mississippiensis, in Barren River Lake, Kentucky. Monthly collections from three areas on …


Neutralization Of Vascular Endothelial Growth Factor Antiangiogenic Isoforms Or Administration Of Proangiogenic Isoforms Stimulates Vascular Development In The Rat Testis, Michelle M. Baltes-Breitwisch, Robin A. Artac, Rebecca C. Bott, Renee M. McFee, Jill G. Kerl, Debra T. Clopton, Andrea S. Cupp 2010 University of Nebraska-Lincoln

Neutralization Of Vascular Endothelial Growth Factor Antiangiogenic Isoforms Or Administration Of Proangiogenic Isoforms Stimulates Vascular Development In The Rat Testis, Michelle M. Baltes-Breitwisch, Robin A. Artac, Rebecca C. Bott, Renee M. Mcfee, Jill G. Kerl, Debra T. Clopton, Andrea S. Cupp

Department of Animal Science: Faculty Publications

Vascular endothelial growth factor A (VEGFA) plays a role in both angiogenesis and seminiferous cord formation, and alternative splicing of the Vegfa gene produces both proangiogenic isoforms and antiangiogenic isoforms (B-isoforms). The objectives of this study were to evaluate the expression of pro- and antiangiogenic isoforms during testis development and to determine the role of VEGFA isoforms in testis morphogenesis. Quantitative RT-PCR determined that Vegfa_165b mRNA was most abundant between embryonic days 13.5 and 16 (E13.5 and 16; P < 0.05). Compared with ovarian mRNA levels, Vegfa_120 was more abundant at E13–14 (P < 0.05), Vegfa_164 was less abundant at E13 (P < 0.05), and Vegfa_165b tended to be less abundant …


Feeding Corn Milling Co-Products To Forage Fed Cattle, 1st Edition, Aaron Stalker, Rick Rasby, Galen E. Erickson, Crystal Buckner, Terry J. Klopfenstein 2010 University of Nebraska-Lincoln

Feeding Corn Milling Co-Products To Forage Fed Cattle, 1st Edition, Aaron Stalker, Rick Rasby, Galen E. Erickson, Crystal Buckner, Terry J. Klopfenstein

University of Nebraska-Lincoln Extension: Historical Materials

Introduction

Beef calves from weaning until they enter feedlots, developing heifers, and beef cows are fed primarily forage diets. Especially in the winter, forages are low in protein and phosphorus and need to be supplemented. Corn milling co-products are excellent sources of both protein and phosphorus and fi t nicely into winter supplementation programs. Corn milling co-products are also an excellent source of energy and are particularly well suited to forage based diets.

Two primary types of milling processes currently exist, resulting in quite diff erent feed products. The dry milling process produces distillers grains plus solubles (DGS), and the …


Insectivorous Bat Assemblages In Relation To Spatial Aspects Of Virgin Jungle Reserves In Peninsular Malaysia., Joann Christine Luruthusamy 2010 Universiti Malaya

Insectivorous Bat Assemblages In Relation To Spatial Aspects Of Virgin Jungle Reserves In Peninsular Malaysia., Joann Christine Luruthusamy

Student Works (2010-2019)

The tropical region supports the highest diversity of bat fauna known. Reasons for their success are mainly due to their large geographical ranges, the diversity of habitats, various foraging strategies and the tendency to exhibit a variety of roosting behaviour. This study addresses the issue of insectivorous bat distribution in relation to spatial aspects such as geography, size, distance and topography. This information was gathered from the 958 individuals and 27 insectivorous bat species that were captured and identified over a span of 18 months. This study which included six virgin jungle reserves of various sizes across Peninsular Malaysia revealed …


Western Rock Lobster International Stock Assessment And Modelling Workshop Report, Department of Fisheries 2010 Department of Primary Industries and Regional Development, Western Australia

Western Rock Lobster International Stock Assessment And Modelling Workshop Report, Department Of Fisheries

Fisheries Occasional Publications

No abstract provided.


Interaction Between Roughages And Corn Milling Byproducts In Finishing Cattle Diets, Joshua R. Benton 2010 University of Nebraska-Lincoln

Interaction Between Roughages And Corn Milling Byproducts In Finishing Cattle Diets, Joshua R. Benton

Department of Animal Science: Dissertations, Theses, and Student Research

During ethanol production, starch is the primary nutrient fermented and the remaining byproducts are excellent sources of fiber and protein. In addition, inclusion of byproducts in finishing diets may reduce the incidence of acidosis. As a result, roughage level and quality could potentially be reduced in finishing diets containing byproducts. Three experiments were conducted to examine the effects of roughage and wet corn gluten feed (WCGF) in finishing cattle diets containing corn distillers grains plus solubles. Cattle fed finishing diets containing wet distillers grains plus solubles (WDGS) with no roughage had decreased DMI and ADG compared to cattle fed roughage. …


Evaluation Of Health And Diabetes Knowledge Of Cu4health Participants By Food Frequency Questionnaire And Michigan Diabetes Knowledge Test, Peter Mukwevho 2010 Clemson University

Evaluation Of Health And Diabetes Knowledge Of Cu4health Participants By Food Frequency Questionnaire And Michigan Diabetes Knowledge Test, Peter Mukwevho

All Theses

Type 2 Diabetes (T2D) has been steadily rising in the United States. The condition is characterized by elevated blood glucose levels and is preceded by insulin resistance. Before the onset of T2D patients go through a state of pre-diabetes. To be tested for pre-diabetes and diabetes, either a Fasting Plasma Glucose test or an Oral Glucose Tolerance Test may be used. While an estimated 24 million Americans have T2D, another 57 million Americans are estimated to be in a state of pre-diabetes. There are many risk factors that may contribute to the development of T2D including obesity, a high calorie …


Information Acquisition And Sociality Among Migratory Birds, Zoltán Németh 2010 University of Southern Mississippi

Information Acquisition And Sociality Among Migratory Birds, Zoltán Németh

Dissertations

Information use is a key feature of adaptive behavior: the better informed an individual, the better it is able to adjust its behavior to meet the demands of a variable world. Therefore, most animals attempt to reduce environmental uncertainty by gathering information when it is available. However, tracking unpredictable ecological factors may carry costs as individuals invest valuable time and energy in the process of information acquisition. Social learning (i.e., use of social information inadvertently produced by other individuals) enables the individual to gain rapid and more complete assessment of its novel environment. This process may be particularly important for …


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