Meat Science Commons^™

Loss Of Vascular Endothelial Growth Factor A (Vegfa) Isoforms In Granulosa Cells Using Pdmrt-1-Cre Or Amhr2-Cre Reduces Fertility By Arresting Follicular Development And By Reducing Litter Size In Female Mice, Kevin M. Sargent, Ningxia Lu, Debra T. Clopton, William E. Pohlmeier, Vanessa Brauer, Napoleone Ferrara, David W. Silversides, Andrea S. Cupp

Department of Animal Science: Faculty Publications

Because VEGFA has been implicated in follicle development, the objective of this study was to determine the effects of granulosa- and germ cell-specific VEGFA loss on ovarian morphogenesis, function, and female fertility. pDmrt1-Cre mice were mated to floxed VEGFA mice to develop granulosa-/germ cell-specific knockouts (pDmrt1-Cre;Vegfa^-/-). The time from mating to first parturition was increased when pDmrt1-Cre;Vegfa^-/- females were mated to control males (P = 0.0008) and tended to be longer for heterozygous females (P < 0.07). Litter size was reduced for pDmrt1-Cre;Vegfa^-/- females (P < 0.007). The time between the first and second parturitions was also increased for heterozygous females (P < 0.04) and tended to be increased for pDmrt1-Cre;Vegfa^-/- females (P < 0.07). pDmrt1-Cre;Vegfa^-/- females had smaller ovaries (P …

Estimation Of Breed-Specific Heterosis Effects For Birth, Weaning, And Yearling Weight In Cattle, Lauren N. Schiermiester, R. M. Thallman, Larry Kuehn, Stephen D. Kachman, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Heterosis, assumed proportional to expected breed heterozygosity, was calculated for 6834 individuals with birth, weaning and yearling weight records from Cycle VII and advanced generations of the U.S. Meat Animal Research Center (USMARC) Germplasm Evaluation (GPE) project. Breeds represented in these data included: Angus, Hereford, Red Angus, Charolais, Gelbvieh, Simmental, Limousin and Composite MARC III. Heterosis was further estimated by proportions of British × British (B × B), British × Continental (B × C) and Continental × Continental (C × C) crosses and by breed-specific combinations. Model 1 fitted fixed covariates for heterosis within biological types while Model 2 fitted …

Bulletin 2220: Best Management Practices For Small Scale Poultry Producers In Maine, Richard Brzozowski, Donna R. Coffin, Michael Darre

Cooperative Extension - Agriculture

Written for small-scale poultry producers. University specialists, Extension educators, and agriculture service providers from New England and New York developed this list of Best Management Practices to assist small-scale poultry growers to provide the best of care for their birds, minimize losses due to disease and predation, as well as minimizing the impact on the environment while assuring a high quality, wholesome product for their family and customers.

Effect Of Lysozyme Or Antibiotics On Faecal Zoonotic Pathogens In Nursery Pigs, J E. Wells, E. D. Berry, N Kalchayanand, L A. Rampel, M Kim, W. T. Oliver

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aims: The objective of this study was to determine the effect of lysozyme and antibiotics on zoonotic pathogen shedding in faeces from nursery pigs housed without and with an indirect disease challenge. Methods and Results: Two replicates of approximately 650 pigs each were weaned and randomly assigned to one of 24 pens in either a nursery room that had been fully disinfected or a nursery room left unclean. Pigs were randomly assigned to control diet (Control), control diet + antibiotics (Antibiotic; chlortetracycline and tiamulin), or control diet + lysozyme (Lysozyme; 100 mg kg ^-1 diet). Rectal swab samples were collected …

Exogenous Administration Of Lipids To Steers Alters Aspects Of The Innate Immune Response To Endotoxin Challenge, Nicole C. Burdick Sanchez, Jeffery A. Carroll, Janet R. Donaldson, Joe O. Buntyn, Ty B. Schmidt

Department of Animal Science: Faculty Publications

This study examined the effects of increasing energy availability from both dextrose and lipid treatments on the proinflammatory response to LPS in Holstein steers. Steers were randomly assigned to one of three groups: saline at 0.5 ml/ kg body weight (Control) or 50% dextrose [0.5 ml/kg body weight (Dextrose) to mimic calm cattle’s response to LPS] administered immediately prior to LPS (0.5 mg/kg body weight at 0 h) or continuous lipid emulsion infusion from –1 to 6 h [Intralipid 20% (Baxter, Deerfield, IL USA); 0.5 ml/kg/hr (Lipid) to mimic temperamental cattle]. Concentrations of non-esterified fatty acids (NEFA) were greater in …

Dehydrated Citrus Pulp Alters Feedlot Performance Of Crossbred Heifers During The Receiving Period And Modulates Serum Metabolite Concentrations Before And After An Endotoxin Challenge, J. T. Cribbs, B. C. Bernhard, T. R. Young, M. A. Jennings, N.C. Burdick Sanchez, J. A. Carroll, T. R. Callaway, T. B. Schmidt, B. J. Johnson, R. J. Rathmann

Department of Animal Science: Faculty Publications

English × Continental heifers (n = 180) were sourced in 2 loads (219.3 ± 16.0 and 221.4 ± 16.4 kg, respectively) from commercial auction barns to study the effects of feeding dehydrated citrus pulp (DCP) on feedlot performance of newly received heifers. A completely randomized block design was used with BW nested within arrival load and blocked by BW into 3 dietary treatments (36 pens, 5 heifers/ pen, 12 blocks, 3 pens/block, and 12 pens/treatment). Treatment diets contained 1) 0% DCP (control diet [CON]), 2) 10% DCP, or 3) 20% DCP on a DM basis. Diets containing DCP were exchanged …

Technical Note: Collection And Preparation Techniques Change Nutrient Composition Of Masticate Collected From Esophageally Fistulated Cattle, Jacqueline A. Musgrave, J. V. Judy, K. H. Jenkins, T. J. Klopfenstein, L. A. Stalker

Department of Animal Science: Faculty Publications

Two experiments determined effects of collection and preparation techniques on nutrient composition of masticate samples from esophageally fistulated cattle. In Exp. 1, 12 esophageally fistulated cattle were maintained on 2 precollection diets, high CP (24% CP; n = 6) or low CP (7.7% CP; n = 6), for 8 d. On d 9, the esophageal plug was removed, screen bottom bags were attached, and each cow was offered fresh grass. Immediately after fresh grass sample collection was complete, dry grass (hay) was offered and a sample was collected. Blood samples were collected and analyzed for serum urea nitrogen concentration. Masticate …

Optimizing The Use Of Fibrous Residues In Beef And Dairy Diets, James C. Macdonald, G. E. Erickson, P. J. Kononoff, T. J. Klopfenstein

Department of Animal Science: Faculty Publications

Increased corn prices over the past decade have altered land use away from traditional forage in favor of corn. Accordingly, beef and dairy producers have had to adopt non-traditional forage resources into their production systems, many of which have become available as a result of increased corn production. Byproducts of the wet and dry milling industries have been used to replace both corn and forage in beef and dairy diets. Byproducts containing corn bran have large amounts of readily digestible hemicellulose. The use of byproducts may increase milk production, ADG, and G:F in dairy, beef growing, and beef finishing diets, …

Yield And Forage Value Of A Dual-Purpose Bmr-12 Sorghum Hybrid, Melinda K. Yerka, Andrea K. Pederson, J. J. Toy, Galen E. Erickson, Jeffrey F. Pedersen, Rob B. Mitchell

Department of Animal Science: Faculty Publications

Grain sorghum [Sorghum bicolor (L.) Moench] is an important crop for rainfed production systems with 2.7 million ha grown in the United States in 2013. The brown-midrib (bmr) mutations, especially bmr-12, have resulted in low stover lignin and high fiber digestibility without reducing grain yield in some sorghum lines. However, the effect of the bmr trait on beef cattle (Bos taurus) performance when grazing crop residue is unknown. Our objectives were to validate previous small-plot results reporting no grain yield difference between near-isogenic bmr-12 (BMR) and wild-type control (CON) A Wheatland × R

Tx430 …

Development Of A Row-Crop Tractor Data Acquisition System, James Roeber, Santosh Pitla, Roger M. Hoy, Joe D. Luck

Department of Animal Science: Faculty Publications

Numerous advancements in machinery performance on row-crop agricultural tractors have been made in recent years. Currently, the Organisation for Economic Co-operation and Development (OECD) only tests predetermined points (E.g., max. power, max. torque) for drawbar and hydraulic power. This method of testing has been used for many years with the tractor at a steady state which is usually not typical of row-crop operations. A data acquisition system (DAQ) was designed that records implement energy consumption (E.g., drawbar loading, PTO power, and hydraulic power). The system used a LabVIEW program and a compact data acquisition system (cDAQ) to record data from …

Mesh Ora Framework: R/Bioconductor Packages To Support Mesh Over-Representation Analysis, Koki Tsuyuzaki, Gota Morota, Manabu Ishii, Takeru Nakazato, Satoru Miyazaki, Itoshi Nikaido

Department of Animal Science: Faculty Publications

Background: In genome-wide studies, over-representation analysis (ORA) against a set of genes is an essential step for biological interpretation. Many gene annotation resources and software platforms for ORA have been proposed. Recently, Medical Subject Headings (MeSH) terms, which are annotations of PubMed documents, have been used for ORA. MeSH enables the extraction of broader meaning from the gene lists and is expected to become an exhaustive annotation resource for ORA. However, the existing MeSH ORA software platforms are still not sufficient for several reasons.

Results: In this work, we developed an original MeSH ORA framework composed of six types of …

Microbial Diversity And Activity In The Nematostella Vectensis Holobiont: Insights From 16s Rrna Gene Sequencing, Isolate Genomes, And A Pilot-Scale Survey Of Gene Expression, Jia Y. Har, Tim Helbig, Ju H. Lim, Samodha C. Fernando, Adam M. Reitzel, Kevin Penn, Janelle R. Thompson

Department of Animal Science: Faculty Publications

We have characterized the molecular and genomic diversity of the microbiota of the starlet sea anemone Nematostella vectensis, a cnidarian model for comparative developmental and functional biology and a year-round inhabitant of temperate salt marshes. Molecular phylogenetic analysis of 16S rRNA gene clone libraries revealed four ribotypes associated with N. vectensis at multiple locations and times. These associates include two novel ribotypes within the ε-Proteobacterial order Campylobacterales and the Spirochetes, respectively, each sharing 99% 16S rRNA identity with Endozoicomonas elysicola and Pseudomonas oleovorans, respectively. Species-specific PCR revealed that these populations persisted in N. vectensis asecually propagated under laboratory …

An Application Of Mesh Enrichment Analysis In Livestock, Gota Morota, F. Moreno, James L. Petersen, Daniel C. Ciobanu, K. Tsuyuzaki, I. Nikaido

Department of Animal Science: Faculty Publications

An integral part of functional genomics studies is to assess the enrichment of specific biological terms in lists of genes found to be playing an important role in biological phenomena. Contrasting the observed frequency of annotated terms with those of the background is at the core of overrepresentation analysis (ORA). Gene Ontology (GO) is a means to consistently classify and annotate gene products and has become a mainstay in ORA. Alternatively, Medical Subject Headings (MeSH) offers a comprehensive life science vocabulary including additional categories that are not covered by GO. Although MeSH is applied predominantly in human and model organism …

Cattle With Increased Severity Of Bovine Respiratory Disease Complex Exhibit Decreased Capacity To Protect Against Histone Cytotoxicity, J. A. Matera, B. K. Krehbiel, J. A. Hernandez Gifford, D. L. Step, C. R. Krehbiel, C. A. Gifford

Department of Animal Science: Faculty Publications

Bovine respiratory disease complex (BRDC) is the leading cause of morbidity and mortality in feedlot cattle. Significant inflammation and lesions are often observed in lungs of infected cattle. During acute inflammatory responses, histones contribute to mortality in rodents and humans and serum proteins can protect against histone-induced cytotoxicity. We hypothesized that cattle experiencing chronic or fatal cases of BRDC have reduced ability to protect against cytotoxic effects of histones. Serum samples were collected from 66 bull calves at the time of normal feedlot processing procedures. Animals were retrospectively assigned to groups consisting of calves never treated for BRDC (control [CONT]; …

The Effects Of Technology Use In Feedlot Production Systems On Feedlot Performance And Carcass Characteristics, C. L. Maxwell, B. C. Wilson, C. F. O'Neill, B. K. Wilson, C. G. Hixon, C. L. Haviland, A. N. Grimes, M. S. Calvo-Lorenzo, D. L. Vanoverbeke, G. G. Mafi, C. J. Richards, D. L. Step, B. P. Holland, C. R. Krehbiel

Department of Animal Science: Faculty Publications

The objectives of this study were to examine the effects of feedlot production systems with and without the use of a β-adrenergic agonist compared to an all-natural production program on feedlot performance and carcass characteristics. Crossbred beef steers (n = 336; initial BW = 379 ± 8 kg) were randomized to 1 of 3 treatments in a randomized complete block design (RCBD; 14 steers/pen; 8 pens/treatment). Treatments consisted of an all-natural treatment (NAT), a conventional treatment (CONV), and a conventional treatment with a β-agonist (CONV-Z). All treatments were fed the same basal diet with NAT cattle receiving no growth promoting …

Effects Of Processing Treated Corn Stover And Distillers Grains On Performance Of Growing Cattle, Jana L. Harding, Curtis J. Bittner, Dirk B. Burken Burken, Galen E. Erickson, James C. Macdonald

Nebraska Beef Cattle Reports

A study evaluated the effects of replacinga diet consisting of 60% corn stover, 18% solubles, and 18% distillers grains with a complete pelleted feed containing calcium oxide (CaO) treated corn stover and distillers grains on growing cattle performance. The pelleted feed was either pair-fed to the control treatment or fed ad libitum. There were no differences in ending BW, ADG, or F:G between the control and pair-fed treatment. Feeding the pellet ad libitum resulted in greater DMI and ADG; however, the cattle had greater F:G. The pellet has 98% the feeding value of the control treatment.

2015 Beef Cattle Report (Complete)

Nebraska Beef Cattle Reports

Table of Contents

Cow/Calf

Impact of Heifer Development System on Subsequent Gain and Reproduction 5

Genetic Parameter Estimates for Calving Difficulty and Birth Weight in a Multibreed Population 8

Estimation of British- and Continental-Specific Heterosis Effects for Birth, Weaning, and Yearling Weight in Cattle 10

Using Sugar Beet Pulp to Replace Wheat Straw when Limit Feeding Late Gestation Beef Cows 12

Supplementing Cow-Calf Pairs Grazing Smooth Bromegrass 14

Effects of Calf Age at Weaning on Cow and Calf Performance and Feed Utilization in an Intensive Production System 16

An Economic Analysis of Conventional and Alternative Cow-Calf Production Systems 19

Effect …

An Economic Analysis Of Conventional And Alternative Cow-Calf Production Systems, Jason M. Warner, Andrea K. Watson, K. H. Jenkins Jenkins, Rick J. Rasby, Kate Brooks, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

Profitability through weaning was predicted for conventional and alternative cow-calf production systems using various input price scenarios. At base input price levels, conventional systems were more economical than alternative systems. As pasture price increased, alternativesystems became cost effective. Feeding cows year-round in a confinement setting appeared the least economical; however, an alternative system combining summer drylot feeding with cornstalk grazing is projected to be economically competitive given an increasing abundance of corn residue.

Effects Of Calf Age At Weaning On Cow And Calf Performance And Feed Utilization In An Intensive Production System, Jason M. Warner, Curtis J. Bittner, Karla H. Jenkins Jenkins, Rick J. Rasby, Matt K. Luebbe, Galen E. Erickson, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

The effects of calf weaning age on cow and calf performance, reproduction, and feed utilization were investigated in a two-year study. Early weaning increasedcow BW in January. Pregnancy rates were not impacted by calf age at weaning. Dry matter intake (DMI) was similar between normal-weaned cow-calf pairs and early-weaned cows and calves. Feed requirements and utilization were comparable between early- and normal-weaned pairs when fed high energy diets, implying weaning decisions should be made on the basis of management rather than feed efficiency.

Effect Of Corn Residue Removal On Subsequent Crop Yields, Mary E. Drewnoski, L. Aaron Stalker, James C. Macdonald Donald, Galen E. Erickson, Kathy J. Hanford, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

Two studies were conducted to evaluate the effects of corn residue harvest on subsequent crop yields. In a long-term study (16 years), cattle grazing corn residue in the spring (February to the middle of April) or the fall (November through January) slightly improved subsequent soybean yields and had no effect on corn yields in an irrigated field maintained in an annual corn-soybean rotation at Mead, Neb. In a five-year study, fall grazing (December through January) or baling of corn residue had no effect on subsequent corn grain yields in a field maintained in continuous corn production at Brule, Neb. These …