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Influence Of Sodium Chloride Reduction And Replacement With Potassium Chloride Based Salts On The Sensory And Physico-Chemical Characteristics Of Pork Sausage Patties, Regan E. Stanley, Chad G. Bower, Gary A. Sullivan 2017 University of Nebraska-Lincoln

Influence Of Sodium Chloride Reduction And Replacement With Potassium Chloride Based Salts On The Sensory And Physico-Chemical Characteristics Of Pork Sausage Patties, Regan E. Stanley, Chad G. Bower, Gary A. Sullivan

Department of Animal Science: Faculty Publications

This study evaluated the effects of sodium chloride reduction and replacement with potassium chloride or modified potassium chloride based salts using a weight or molar equivalent basis on the sensory and physicochemical properties of pork sausage patties. Three independent replications of pork sausage patties were manufactured to compare five treatments: full sodium, reduced sodium, modified potassium chloride weight based replacement, modified potassium chloride molar based replacement, and standard potassium chloride weight based replacement. Salt replacement did not affect (P > 0.05) moisture, protein, fat, textural properties, lipid oxidation, or redness. Sausage patties with modified potassium chloride were more acceptable than those …


Evaluation Of The National Research Council (2001) Dairy Model And Derivation Of New Prediction Equations. 1. Digestibility Of Fiber, Fat, Protein, And Nonfiber Carbohydrate, R. R. White, Y. Roman-Garcia, J. L. Firkins, M. J. VandeHaar, L. E. Armentano, W. P. Weiss, T. McGill, R. Garnett, M. D. Hanigan 2017 Virginia Tech

Evaluation Of The National Research Council (2001) Dairy Model And Derivation Of New Prediction Equations. 1. Digestibility Of Fiber, Fat, Protein, And Nonfiber Carbohydrate, R. R. White, Y. Roman-Garcia, J. L. Firkins, M. J. Vandehaar, L. E. Armentano, W. P. Weiss, T. Mcgill, R. Garnett, M. D. Hanigan

Department of Animal Science: Faculty Publications

Evaluation of ration balancing systems such as the National Research Council (NRC) Nutrient Requirementsseries is important for improving predictions of animal nutrient requirements and advancing feeding strategies. This work used a literature data set (n = 550) to evaluate predictions of total-tract digested neutral detergent fiber (NDF), fatty acid (FA), crude protein (CP), and nonfiber carbohydrate (NFC) estimated by the NRC (2001) dairy model. Mean biases suggested that the NRC (2001) lactating cow model overestimated true FA and CP digestibility by 26 and 7%, respectively, and under-predicted NDF digestibility by 16%. All NRC (2001) estimates had notable mean and …


The Effect Of Regular Or Reduced-Fat Distillers Grains With Solubles On Rumen Methanogenesis And The Rumen Bacterial Community, E. Castillo-Lopez, C. J. R. Jenkins, N. D. Aluthge, W. Tom, P. J. Kononoff, S. C. Fernando 2017 Universidad Nacional Autonoma de Mexico

The Effect Of Regular Or Reduced-Fat Distillers Grains With Solubles On Rumen Methanogenesis And The Rumen Bacterial Community, E. Castillo-Lopez, C. J. R. Jenkins, N. D. Aluthge, W. Tom, P. J. Kononoff, S. C. Fernando

Department of Animal Science: Faculty Publications

Aims: The effect of feeding dried distillers grains with solubles (DDGS) or reduced-fat DDGS (RFDG) on ruminal methanogenesis and the rumen bacterial community of dairy cattle was evaluated. Methods and Results: Treatments were CONT, a diet with no distillers grains; DG, inclusion of 20% DDGS; rfDG, inclusion of 20% RFDG; and MIX, inclusion of 10% DDGS and 10% RFDG. Methane emission was measured; rumen bacterial community was evaluated by sequencing the V4 region of the 16S rRNA gene. Total methane production remained unaffected. However, feeding distillers grains tended to reduce methanogenesis per unit of feed intake, decreased the abundance of …


Effect Of Feeding Distillers Grains During Different Phases Of Production And Addition Of Postmortem Antioxidants On Shelf Life Of Ground Beef1, B. D. Cleveland, J. O. Buntyn, A. L. Gronli, J. C. MacDonald, University of Nebraska - Lincoln, G. A. Sullivan 2017 University of Nebraska - Lincoln

Effect Of Feeding Distillers Grains During Different Phases Of Production And Addition Of Postmortem Antioxidants On Shelf Life Of Ground Beef1, B. D. Cleveland, J. O. Buntyn, A. L. Gronli, J. C. Macdonald, University Of Nebraska - Lincoln, G. A. Sullivan

Department of Animal Science: Faculty Publications

Feeding distillers grains (DGS) to cattle can increase PUFA concentration, increase lipid oxidation, and de- crease color stability of beef. The objective of this study was to evaluate the effects of feeding DGS and the post- mortem addition of antioxidants on the shelf life of ground beef products. Crossbred heifers (n = 64; initial BW = 225 kg) were supplemented with different amounts of modi- fied DGS (MDGS; 0.91 or 2.27 kg daily, DM basis) dur- ing backgrounding and finished on diets containing corn gluten feed or MDGS. Four beef shoulder clods from each dietary group were ground independently. Fatty …


In Situ Digestibility Of Residue Parts Of Corn Planted In Different Populations And Row Widths, Viviane B. Ferrari, Janessa J. Updike, Jana Harding Harding, Keith Glewen, Terry J. Klopfenstein, Jim C. MacDonald 2017 University of Nebraska - Lincoln

In Situ Digestibility Of Residue Parts Of Corn Planted In Different Populations And Row Widths, Viviane B. Ferrari, Janessa J. Updike, Jana Harding Harding, Keith Glewen, Terry J. Klopfenstein, Jim C. Macdonald

Nebraska Beef Cattle Reports

Plant density can be changed by altering row width and/or number of plants within a row. The use of narrower rows at seeding may increase yield and reduce plant-toplant competition. Corn seeds were planted at 2 row widths (15 and 30- inch rows) and 3 plant populations (25,200, 35,000 and 54,000 seeds / acre). Samples of corn residue were collected 4 dates (October 8, October 24, November 5 and November 19) postharvest and separated into cob, stem, leaf and husk to determine changes in quality over time. Th e greatest plant population increased the NDF content and NDF digestibility of …


144 Effect Of Antral Follicle Count In Beef Heifers On In Vitro Fertilization/Production, C. C. Chase Jr., R. A. Freeland, A. K. McNeel, E. C. Wright-Johnson, G. A. Perry, S. C. Tenley, J. R. Wood, A. S. Cupp, J. L. Vallet, J. R. Miles 2017 USDA-ARS

144 Effect Of Antral Follicle Count In Beef Heifers On In Vitro Fertilization/Production, C. C. Chase Jr., R. A. Freeland, A. K. Mcneel, E. C. Wright-Johnson, G. A. Perry, S. C. Tenley, J. R. Wood, A. S. Cupp, J. L. Vallet, J. R. Miles

Department of Animal Science: Faculty Publications

Our objective has been to compare the IVF and in vitro production (IVP) of embryos from low and high antral follicle count (AFC) heifers. This is the fourth year of the study with years 1 to 3 reported individually. For this report, we add data for the fourth year and present a combined analysis (years 1 to 4) for the first time. Each year, AFC was determined on ~120 Angus heifers using transrectal ultrasonography. Ten heifers with the lowest AFC and 10 heifers with the highest AFC and all with evidence of oestrous cyclicity were synchronized with two 5-mL injections …


Medical Subject Heading (Mesh) Annotations Illuminate Maize Genetics And Evolution, Timothy M. Beissinger, Gota Morota 2017 University of Missouri, Columbia

Medical Subject Heading (Mesh) Annotations Illuminate Maize Genetics And Evolution, Timothy M. Beissinger, Gota Morota

Department of Animal Science: Faculty Publications

Background: High-density marker panels and/or whole-genome sequencing, coupled with advanced phenotyping pipelines and sophisticated statistical methods, have dramatically increased our ability to generate lists of candidate genes or regions that are putatively associated with phenotypes or processes of interest. However, the speed with which we can validate genes, or even make reasonable biological interpretations about the principles underlying them, has not kept pace. A promising approach that runs parallel to explicitly validating individual genes is analyzing a set of genes together and assessing the biological similarities among them. This is often achieved via gene ontology analysis, a powerful tool that …


Production Of A Gonadotropin-Releasing Hormone 2 Receptor Knockdown (Gnrhr2 Kd) Swine Line, A. T. Desaulniers, R. A. Hou, G. A. Mills, C. A. Lents, Brett R. White 2017 University of Nebraska-Lincoln

Production Of A Gonadotropin-Releasing Hormone 2 Receptor Knockdown (Gnrhr2 Kd) Swine Line, A. T. Desaulniers, R. A. Hou, G. A. Mills, C. A. Lents, Brett R. White

Department of Animal Science: Faculty Publications

Swine are the only livestock species that produce both the second mammalian isoform of gonadotropin-releasing hormone (GNRH2) and its receptor (GNRHR2). Previously, we reported that GNRH2 and GNRHR2 mediate LH-independent testosterone secretion from porcine testes. To further explore this ligand-receptor complex, a pig model with reduced GNRHR2 expression was developed. Small hairpin RNA sequences targeting porcine GNRHR2 were subcloned into a lentiviral-based vector, lentiviral particles were generated and microinjected into the perivitelline space of zygotes, and embryos were transferred into a recipient. One GNRHR2 knockdown (KD) female was born that subsequently produced 80 piglets from 6 litters with 46 hemizygous …


Physically Adjusted Neutral Detergent Fiber System For Lactating Dairy Cow Rations. I: Deriving Equations That Identify Factors That Influence Effectiveness Of Fiber, Robin R. White, Mary Beth Hou, Jeffrey L. Firkins, Paul J. Kononoff 2017 Virginia Tech

Physically Adjusted Neutral Detergent Fiber System For Lactating Dairy Cow Rations. I: Deriving Equations That Identify Factors That Influence Effectiveness Of Fiber, Robin R. White, Mary Beth Hou, Jeffrey L. Firkins, Paul J. Kononoff

Department of Animal Science: Faculty Publications

Physically effective neutral detergent fiber (peNDF) is the fraction of neutral detergent fiber (NDF) that stimulates chewing activity and contributes to the floating mat of large particles in the rumen. Multiplying dietary NDF by particle size has been used as an estimate of peNDF. In re-evaluating the concept of peNDF, we compared the use of peNDF as dietary NDF × particle size with the use of individual NDF and particle size descriptors (physically adjusted NDF; paNDF) when used with other physical and chemical diet descriptors to predict dry matter (DM) intake (DMI), rumination time, and ruminal pH in lactating dairy …


Expression Of Cytokine Genes And Receptors In White Blood Cells Associated With Divergent Body Weight Gain In Beef Steers, Amanda K. Lindholm-Perry, Virginia M. Cederberg, Jeremy R. Miles, Andrew P. Foote 2017 USDA-ARS

Expression Of Cytokine Genes And Receptors In White Blood Cells Associated With Divergent Body Weight Gain In Beef Steers, Amanda K. Lindholm-Perry, Virginia M. Cederberg, Jeremy R. Miles, Andrew P. Foote

Department of Animal Science: Faculty Publications

Previous work examining the transcriptome of steer tissue samples from animals with divergent gain have shown a relationship with the expression of genes with functions in immune and inflammatory pathways. The process of mounting an immune or inflammatory response is energetically expensive and variation in cytokine responses may affect cattle production traits. In addition, a previous study has identified variation in the transcript abundance of numerous genes, including the cytokine gene IL6ST, in the circulating white blood cells of pigs associated with high and low residual feed intake (RFI) lines. The aim of this study was to determine whether …


Economic Selection Index Development For Beefmaster Cattle I: Terminal Breeding Objective, Kathleen P. Ochsner, M. D. MacNeil, Matthew L. Spangler 2017 University of Nebraska-Lincoln

Economic Selection Index Development For Beefmaster Cattle I: Terminal Breeding Objective, Kathleen P. Ochsner, M. D. Macneil, Matthew L. Spangler

Department of Animal Science: Faculty Publications

The objective of this study was to develop an economic selection index for Beefmaster cattle in a terminal production system where bulls are mated to mature cows with all resulting progeny harvested. National average prices from 2010 to 2014 were used to establish income and expenses for the system. Phenotypic and genetic parameter values among the selection criteria and goal traits were obtained from literature. Economic values were estimated by simulating 100,000 animals and approximating the partial derivatives of the profit function by perturbing traits one at a time, by 1 unit, while holding the other traits constant at their …


The Impact Of Training Strategies On The Accuracy Of Genomic Predictors In United States Red Angus Cattle, J. Lee, Stephen D. Kachman, Matthew L. Spangler 2017 University of Nebraska-Lincoln

The Impact Of Training Strategies On The Accuracy Of Genomic Predictors In United States Red Angus Cattle, J. Lee, Stephen D. Kachman, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Genomic selection (GS) has become an integral part of genetic evaluation methodology and has been applied to all major livestock species, including beef and dairy cattle, pigs, and chickens. Significant contributions in increased accuracy of selection decisions have been clearly illustrated in dairy cattle after practical application of GS. In the majority of U.S. beef cattle breeds, similar efforts have also been made to increase the accuracy of genetic merit estimates through the inclusion of genomic information into routine genetic evaluations using a variety of methods. However, prediction accuracies can vary relative to panel density, the number of folds used …


Genomics Symposium: Using Genomic Approaches To Uncover Sources Of Variation In Age At Puberty And Reproductive Longevity In Sows, Hiruni R. Wijesena, Clay A. Lents, Jean-Jack Riethoven, Melanie D. Trenhaile-Grannemann, Jennifer F. Thorson, Brittney N. Keel, Phillip S. Miller, Matt Spangler, Stephen D. Kachman, Daniel C. Ciobanu 2017 University of Nebraska-Lincoln

Genomics Symposium: Using Genomic Approaches To Uncover Sources Of Variation In Age At Puberty And Reproductive Longevity In Sows, Hiruni R. Wijesena, Clay A. Lents, Jean-Jack Riethoven, Melanie D. Trenhaile-Grannemann, Jennifer F. Thorson, Brittney N. Keel, Phillip S. Miller, Matt Spangler, Stephen D. Kachman, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Genetic variants associated with traits such as age at puberty and litter size could provide insight into the underlying genetic sources of variation impacting sow reproductive longevity and productivity. Genomewide characterization and gene expression profiling were used using gilts from the University of Nebraska–Lincoln swine resource population (n = 1,644) to identify genetic variants associated with age at puberty and litter size traits. From all reproductive traits studied, the largest fraction of phenotypic variation explained by the Porcine SNP60 BeadArray was for age at puberty (27.3%). In an evaluation data set, the predictive ability of all SNP from highranked 1-Mb …


The Roles Of Age At Puberty And Energy Restriction |In Sow Reproductive Longevity: A Genomic Perspective, Hiruni R. Wijesena, Clay A. Lents, Melanie D. Trenhaile-Grannemann, Jean-Jack Riethoven, Brittney N. Keel, Jennifer F. Thorson, Phillip S. Miller, Rodger K. Johnson, Matthew L. Spangler, Stephen D. Kachman, Daniel C. Ciobanu 2017 University of Nebraska-Lincoln

The Roles Of Age At Puberty And Energy Restriction |In Sow Reproductive Longevity: A Genomic Perspective, Hiruni R. Wijesena, Clay A. Lents, Melanie D. Trenhaile-Grannemann, Jean-Jack Riethoven, Brittney N. Keel, Jennifer F. Thorson, Phillip S. Miller, Rodger K. Johnson, Matthew L. Spangler, Stephen D. Kachman, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Approximately 50% of sows are culled annually with more than one-third due to poor fertility. Our research demonstrated that age at puberty is an early pre-breeding indicator of reproductive longevity. Age at puberty can be measured early in life, has a moderate heritability, and is negatively correlated with lifetime number of parities. Detection of age at puberty is tedious and time consuming and is therefore not collected by the industry, which limits genetic progress. Genomic prediction is a viable approach to preselect gilts that will express puberty early and have superior reproductive longevity. The hypothesis that genetic variants explaining differences …


Economic Selection Index Development For Beefmaster Cattle Ii: General-Purpose Breeding Objective, Kathleen P. Ochsner, M. D. MacNeil, Ronald M. Lewis, Matthew L. Spangler 2017 University of Nebraska-Lincoln

Economic Selection Index Development For Beefmaster Cattle Ii: General-Purpose Breeding Objective, Kathleen P. Ochsner, M. D. Macneil, Ronald M. Lewis, Matthew L. Spangler

Department of Animal Science: Faculty Publications

An economic selection index was developed for Beefmaster cattle in a general-purpose production system in which bulls are mated to a combination of heifers and mature cows, with resulting progeny retained as replacements or sold at weaning. National average prices from 2010 to 2014 were used to establish income and expenses for the system. Genetic parameters were obtained from the literature. Economic values were estimated by simulating 100,000 animals and approximating the partial derivatives of the profit function by perturbing traits 1 at a time, by 1 unit, while holding the other traits constant at their respective means. Relative economic …


Development Of An Objective Feet And Leg Conformation Evaluation Method Using Digital Imagery In Swine, J. D. Stock, J. A. Calderon Diaz, C. E. Abell, T. J. Baas, M. F. Rothschild, B. E. Mote, K. J. Stalder 2017 Iowa State University

Development Of An Objective Feet And Leg Conformation Evaluation Method Using Digital Imagery In Swine, J. D. Stock, J. A. Calderon Diaz, C. E. Abell, T. J. Baas, M. F. Rothschild, B. E. Mote, K. J. Stalder

Department of Animal Science: Faculty Publications

Background:The objectives of this study were to create an objective measurement method of joint angles for knee, hock, front and rear pasterns and a rear stance position in swine using digital imaging technology and to assess the repeatability of the objective measurement process. Methods and Findings: Forty-five multiparous sows (average parity 6.7 ± 2.5; parity range 5 to 14) from two commercial farms (n=21 farm 1 and n=24 farm 2) were used. Sows were moved to a pen where digital images of the profile and rear stance were captured. On average, 5.2 (± 2.6) profile and 2.6 (± 1.0) …


Effect Of Partially Replacing A Barley-Based Concentrate With Flaxseed-Based Products On The Rumen Bacterial Population Of Lactating Holstein Dairy Cows, E. Castillo-Lopez, J. Moats, N. D. Aluthge, H. A. Ramirez Ramirez, D. A. Christensen, T. Mutsvangwa, G. B. Penner, S. C. Fernando 2017 University of Saskatchewan

Effect Of Partially Replacing A Barley-Based Concentrate With Flaxseed-Based Products On The Rumen Bacterial Population Of Lactating Holstein Dairy Cows, E. Castillo-Lopez, J. Moats, N. D. Aluthge, H. A. Ramirez Ramirez, D. A. Christensen, T. Mutsvangwa, G. B. Penner, S. C. Fernando

Department of Animal Science: Faculty Publications

Aims: The effects of partial replacement of a barley-based concentrate with flaxseed-based products on the rumen bacterial population of lactating Holstein dairy cows were evaluated. Methods and Results: Treatments fed were CONT, a normal diet that included barley silage, alfalfa hay and a barley-based concentrate that contained no flaxseed or faba beans; FLAX, inclusion of a nonextruded flaxseed-based product containing 55-0% flaxseed, 37-8% field peas and 6-9% alfalfa; EXT, similar to FLAX, but the product was extruded and EXTT, similar to FLAX, but product was extruded and field peas were replaced by high-tannin faba beans. The rumen bacterial population was …


Genomic Relatedness Strengthens Genetic Connectedness Across Management Units, Haipeng Yu, Matthew L. Spangler, Ronald M. Lewis, Gota Morota 2017 University of Nebraska - Lincoln

Genomic Relatedness Strengthens Genetic Connectedness Across Management Units, Haipeng Yu, Matthew L. Spangler, Ronald M. Lewis, Gota Morota

Department of Animal Science: Faculty Publications

Genetic connectedness refers to a measure of genetic relatedness across management units (e.g., herds and flocks). With the presence of high genetic connectedness in management units, best linear unbiased prediction (BLUP) is known to provide reliable comparisons between estimated genetic values. Genetic connectedness has been studied for pedigree-based BLUP; however, relatively little attention has been paid to using genomic information to measure connectedness. In this study, we assessed genomebased connectedness across management units by applying prediction error variance of difference (PEVD), coefficient of determination (CD), and prediction error correlation r to a combination of computer simulation and real data (mice …


National Beef Quality Audit-2016: Transportation, Mobility, And Harvest-Floor Assessments Of Targeted Characteristics That Affect Quality And Value Of Cattle, Carcasses, And By-Products, L.C. Eastwood, C A. Boykin, M.K. Harris, A.N. Arnold, D.S. Hale, C R. Kerth, D.B. Griffin, J.W. Savell, K.E. Belk, D.R. Woerner, J.D. Hasty, R.J. Delmore Jr., J.N. Martin, T.E. Lawrence, T.J. McEvers, D.L. VanOverbeke, G.G. Mafi, M.M. Pfeiffer, Ty B. Schmidt, R.J. Maddock, D.D. Johnson, C.C. Carr, J. M. Scheffler, T.D. Pringle, A.M. Stelzleni 2017 Texas A&M University

National Beef Quality Audit-2016: Transportation, Mobility, And Harvest-Floor Assessments Of Targeted Characteristics That Affect Quality And Value Of Cattle, Carcasses, And By-Products, L.C. Eastwood, C A. Boykin, M.K. Harris, A.N. Arnold, D.S. Hale, C R. Kerth, D.B. Griffin, J.W. Savell, K.E. Belk, D.R. Woerner, J.D. Hasty, R.J. Delmore Jr., J.N. Martin, T.E. Lawrence, T.J. Mcevers, D.L. Vanoverbeke, G.G. Mafi, M.M. Pfeiffer, Ty B. Schmidt, R.J. Maddock, D.D. Johnson, C.C. Carr, J. M. Scheffler, T.D. Pringle, A.M. Stelzleni

Department of Animal Science: Faculty Publications

The National Beef Quality Audit-2016 (NBQA-2016) was conducted to assess current transportation, mobility, and quality characteristics of U.S. fed steers and heifers. Data were collected at 17 beef processing facilities between March and November 2016. About 8,000 live cattle were evaluated for transportation and mobility, and about 25,000 carcasses were evaluated on the slaughter floor. Cattle were in transit to the slaughter facility for a mean duration of 2.7 h from a mean distance of 218.5 km using trailers with dimensions ranging from 17.84 m2 to 59.09 m2. Area allotted per animal averaged 1.13 m2 and ranged from 0.85 m2 …


National Beef Quality Audit–2016: Transportation, Mobility, Live Cattle, And Carcass Assessments Of Targeted Producer-Related Characteristics That Affect Value Of Market Cows And Bulls, Their Carcasses, And Associated By-Products, M.K. Harris, L.C. Eastwood, C.A. Boykin, A.N. Arnold, K.B. Gehring, D.S. Hale, C.R. Kerth, D.B. Griffin, J.W. Savell, K.E. Belk, D.R. Woerner, J.D. Hasty, R.J. Delmore Jr., J.N. Martin, T.E. Lawrence, T.J. McEvers, D.L. VanOverbeke, G.G. Mafi, M.M. Pfeiffer, Ty B. Schmidt, R.J. Maddock, D.D. Johnson, C.C. Carr, J.M. Scheffler, T.D. Pringle, A.M. Stelzleni 2017 Texas A&M University

National Beef Quality Audit–2016: Transportation, Mobility, Live Cattle, And Carcass Assessments Of Targeted Producer-Related Characteristics That Affect Value Of Market Cows And Bulls, Their Carcasses, And Associated By-Products, M.K. Harris, L.C. Eastwood, C.A. Boykin, A.N. Arnold, K.B. Gehring, D.S. Hale, C.R. Kerth, D.B. Griffin, J.W. Savell, K.E. Belk, D.R. Woerner, J.D. Hasty, R.J. Delmore Jr., J.N. Martin, T.E. Lawrence, T.J. Mcevers, D.L. Vanoverbeke, G.G. Mafi, M.M. Pfeiffer, Ty B. Schmidt, R.J. Maddock, D.D. Johnson, C.C. Carr, J.M. Scheffler, T.D. Pringle, A.M. Stelzleni

Department of Animal Science: Faculty Publications

The National Beef Quality Audit–2016 marks the fourth iteration in a series assessing the quality of live beef and dairy cows and bulls and their carcass counterparts. The objective was to determine the incidence of producer-related defects, and report cattle and carcass traits associated with producer management. Conducted from March through December of 2016, trailers (n = 154), live animals (n = 5,470), hide-on carcasses (n = 5,278), and hide-off hot carcasses (n = 5,510) were surveyed in 18 commercial packing facilities throughout the United States. Cattle were allowed 2.3 m2 of trailer space on average during transit indicating some …


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