Evaluating Accuracy Of Dna Pool Construction Based On White Blood Cell Counts,
2021
South Dakota State University
Evaluating Accuracy Of Dna Pool Construction Based On White Blood Cell Counts, Amy N. Abrams, T. G. Mcdaneld, John W. Keele, Carol G. Chitko-Mckown, Larry A. Kuehn, Michael G. Gonda
Roman L. Hruska U.S. Meat Animal Research Center: Reports
Pooling individual samples prior to DNA extraction can mitigate the cost of DNA extraction and genotyping; however, these methods need to accurately generate equal representation of individuals within pools. The objective of this study was to determine accuracy of pool construction of blood samples based on white blood cell counts compared to two common DNA quantification methods. Fifty individual bovine blood samples were collected, and then pooled with all individuals represented in each pool. Pools were constructed with the target of equal representation of each individual animal based on number of white blood cells, spectrophotometric readings, spectrofluorometric readings, and whole …
Rapid Estimation Of Salmonella Enterica Contamination Level In Ground Beef – Application Of The Time-To-Positivity Method Using A Combination Of Molecular Detection And Direct Plating,
2021
USDA ARS Roman L. Hruska U.S. Meat Animal Research Center
Rapid Estimation Of Salmonella Enterica Contamination Level In Ground Beef – Application Of The Time-To-Positivity Method Using A Combination Of Molecular Detection And Direct Plating, Dayna M. Harhay, Margaret D. Weinroth, James L. Bono, Gregory P. Harhay, Joseph M. Bosilevac
Roman L. Hruska U.S. Meat Animal Research Center: Reports
Little progress has been made in decreasing the incidence rate of salmonellosis in the US over the past decade. Mitigating the contribution of contaminated raw meat to the salmonellosis incidence rate requires rapid methods for quantifying Salmonella, so that highly contaminated products can be removed before entering the food chain. Here we evaluated the use of Time-to-Positivity (TTP) as a rapid, semi-quantitative approach for estimating Salmonella contamination levels in ground beef. Growth rates of 14 Salmonella strains (inoculated at log 1 to −2 CFU/g) were characterized in lean ground beef mTSB enrichments and time-to-detection was determined using culture and molecular …
Sdsu Beef Day 2021 Complete Proceedings,
2021
South Dakota State University
Sdsu Beef Day 2021 Complete Proceedings, Department Of Animal Science, Sdsu Extension
SDSU Beef Day 2021 Summary Publication
This is the complete proceedings of the South Dakota State University, Department of Animal Science Beef Day 2021. The proceedings contain research presented on the following topics:
Feedlot
Extension
Herd Management
Meat Science and Food Safety
Assessment Of Alternative Models For Genetic Analysis Of Worm And Tick Infestation In Nellore Cattle,
2021
University of Wisconsin-Madison
Assessment Of Alternative Models For Genetic Analysis Of Worm And Tick Infestation In Nellore Cattle, T. L. Passafaro, F. B. Lopes, Thomas W. Murphy, B. D. Valente, R. C. Leite, G. J.M. Rosa, F. L.B. Toral
Roman L. Hruska U.S. Meat Animal Research Center: Reports
Worms and ticks are important parasites in beef cattle, especially in tropical areas, causing significant economic and production losses. Understanding animal-to-animal variation on infestation for these parasites might guide genetic selection and improvement of management practices to attenuate its detrimental effects. Statistical models used to analyze such traits usually assume a Gaussian distribution for the observed data. However, this assumption is quite often inappropriate for counting data. Therefore, the objectives of this study were: 1) Estimate genetic parameters for worms and tick infestations in Nellore cattle, and 2) To compare the overall performance of six data analysis approaches for worm …
Evaluation Of Kws Hybrid Rye On Growth Performance, Carcass Traits, And Efficiency Of Net Energy Utilization In Finishing Feedlot Steers,
2021
South Dakota State University
Evaluation Of Kws Hybrid Rye On Growth Performance, Carcass Traits, And Efficiency Of Net Energy Utilization In Finishing Feedlot Steers, Warren Rusche, Julie Walker, Southeast South Dakota Research Farm, Becca Brattain, Zachary Smith
SDSU Beef Day 2021 Summary Publication
Objective
Our objectives were to determine the effects of hybrid rye inclusion on finishing steer performance and carcass characteristics, and to estimate net energy (NE) value.
Study Description
Predominately Angus steers (n = 240, initial shrunk bodyweight [BW] = 891 ± 40.8 pounds) were used in 117-d finishing experiment. Hybrid rye was used to replace either one-third, two-thirds, or 100% of dry-rolled corn (DRC) resulting in four treatments: [DRC:Rye, DM basis (60:0), (40:20), (20:40), and (0:60)] with six pens per treatment and 10 steers/pen. Performance adjusted NE values were calculated from performance and carcass data. Replacing DRC with rye linearly …
Effect Of Corn Silage Inclusion Level And Steroidal Implant Type On Growth Performance, Dietary Net Energy Utilization, Beef Production Per Acre And Apparent Total Tract Digestibility In Feedlot Finishing Steers,
2021
South Dakota State University
Effect Of Corn Silage Inclusion Level And Steroidal Implant Type On Growth Performance, Dietary Net Energy Utilization, Beef Production Per Acre And Apparent Total Tract Digestibility In Feedlot Finishing Steers, Elizabeth Buckhaus, Warren Rusche, Zachary Smith
SDSU Beef Day 2021 Summary Publication
Objective
The experimental objective was to determine the influence corn silage inclusion level and terminal implant type have on animal performance, carcass traits, and beef production per acre of cropland in finishing steers harvested at a common fatness endpoint.
Study Description
Maine-Anjou × Angus beef steers (n = 156 steers; initial BW 807 ± 81.9 lbs) were used in a 2 × 2 factorial arrangement finishing experiment at the Ruminant Nutrition Center in Brookings, SD. Steers were weighed on 2 consecutive days and assigned into 5 weight blocks. Dietary treatments consisted (DM basis) of 1) 15% (CS15) or 2) 30% …
Evaluation Of Bacillus Subtilis Pb6 Probiotic (Clostat®500) On Feedlot Phase Growth Performance, Efficiency Of Dietary Net Energy Utilization, And Fecal And Subiliac Lymph Node Salmonella Prevalence In Spring Placement Yearling Beef Steers Fed In Southeastern South Dakota,
2021
South Dakota State University
Evaluation Of Bacillus Subtilis Pb6 Probiotic (Clostat®500) On Feedlot Phase Growth Performance, Efficiency Of Dietary Net Energy Utilization, And Fecal And Subiliac Lymph Node Salmonella Prevalence In Spring Placement Yearling Beef Steers Fed In Southeastern South Dakota, Zachary K. Smith, P. Rand Broadway, Warren C. Rusche, Julie A. Walker, Keith R. Underwood, Jeri;Yn E. Hergenreder
SDSU Beef Day 2021 Summary Publication
Objective
The objective of this research was to determine the influence of Bacillus subtilis PB6 administration had on growth performance, carcass traits, and Salmonella prevalence in yearling steers placed on feed in March in southeastern South Dakota that were not subjected to marketing or environmental stressors during the finishing phase.
Study Description
Yearling crossbred beef steers (N = 238; initial shrunk BW = 886 ± 68.8 lbs) were used in a 140-d finishing study at the Southeast Research Farm (SERF) in Beresford, SD. Steers were allotted to one of 24 pens (N = 9 to 10 steers/pen) and assigned to …
Effects Of Harvest Maturity And/Or Kernel Processing On Corn Silage Processing Score And Particle Size Of Corn Silage,
2021
South Dakota State University
Effects Of Harvest Maturity And/Or Kernel Processing On Corn Silage Processing Score And Particle Size Of Corn Silage, Thomas Hamilton, Julie Walker, Warren Rusche, Zachary Smith
SDSU Beef Day 2021 Summary Publication
Objective
A single corn hybrid was used to evaluate harvest maturity (Mat) and/or kernel processing (KP) effects on corn silage processing score (CSPS) and particle size (PS).
Study Description
Treatments were arranged in a 2 × 2 factorial of: 1) Mat (early and late) and 2) KP (no or yes). A single corn field was planted on April 27, 2020. There were 12 loads (experimental unit) per simple effect treatment mean. Data were analyzed as a completely randomized design. Early harvest (3/4 milk line) occurred on August 28, 2020 [yield (as is) = 17.40 tons/acre; DM = 43.1%; CP, NDF, …
Estimating The Effects Of Weather, Dry Matter Intake, And Body Weight On Daily Water Intake In Weaned Calves,
2021
South Dakota State University
Estimating The Effects Of Weather, Dry Matter Intake, And Body Weight On Daily Water Intake In Weaned Calves, Zach S. Mcdaniel, Himali Wickramasinghe, Cody L. Wright, Michael G. Gonda, Zachary K. Smith
SDSU Beef Day 2021 Summary Publication
Objective
The purpose of this study was to study the effect of weather, dry matter intake, and body weight on the water requirements of weaned calves and estimating the requirements in a model.
Study Description
Weaned steers (n=48) were selected to study the effects of the weather, body weight, and dry matter intake on water intake in the winter (n=24) and summer (n=24) months. Calves were provided with ad libitum access to feed and water at the SDSU Cow-Calf Education and Research Facility (CCERF) and measured utilizing an automated feed and water system (Insentec, The Hague, Netherlands). Temperature, humidity, precipitation, …
Virtual Feedlot Shortcourse: When Life Hands Out Lemons,
2021
South Dakota State University, SDSU Extension
Virtual Feedlot Shortcourse: When Life Hands Out Lemons, Warren Rusche
SDSU Beef Day 2021 Summary Publication
Objective
The COVID-19 pandemic forced changes in how Extension programming was delivered in 2020. Web-based distance learning tools were used to deliver educational material when it was impractical to use traditional delivery methods. Study Description
The SDSU Extension Feedlot Shortcourse has traditionally been an in-person event with as much opportunity for hands-on learning and demonstrations as possible. The program is offered over a two-day period in August at the SDSU Cow-Calf Education and Research Facility with approximately 30 participants each year, on average. The program addresses feed delivery and mixing, animal health, production technologies, and risk management. However, the events …
North Central Farm And Ranch Stress Assistance Center: A Comprehensive Needs Assessment In South Dakota,
2021
South Dakota State University
North Central Farm And Ranch Stress Assistance Center: A Comprehensive Needs Assessment In South Dakota, Krista Ehlert, Andrea Bjornestad
SDSU Beef Day 2021 Summary Publication
Objective
The overall objective of this project is to work with mobilized partners to form the North Central Farm and Ranch Stress Assistance Center (NC FRSAC). The NC FRSAC will create and expand stress management and mental health resources and services to agricultural producers, advocates and stakeholders who support agricultural producers. This project is led by the University of Illinois Urbana-Champaign. South Dakota State University is one of the partners and our supporting objective is to establish a state-wide task force to develop an assistance network, conduct a comprehensive needs assessment, develop and implement educational workshops to producers and their …
Developing Web Application Tools To Support Beef Cattle Production,
2021
South Dakota State University
Developing Web Application Tools To Support Beef Cattle Production, Jameson Brennan, Krista Ehlert
SDSU Beef Day 2021 Summary Publication
Objective
The objective of this project was to demonstrate the development of web-based applications to help producers gather and utilize data to make more informed management decisions for their operation.
Vaccinating The Young Calf With A Parenteral Adjuvanted Vaccine To Develop A Protective Brsv Iga Nasal Response,
2021
South Dakota State University
Vaccinating The Young Calf With A Parenteral Adjuvanted Vaccine To Develop A Protective Brsv Iga Nasal Response, Karim W. Abdelsalam, Elizabeth A. Kolb, Robin E. Buterbaugh, Carol L. Rinehart, Douglas Ensley, George A. Perry, Christopher C.L. Chase
SDSU Beef Day 2021 Summary Publication
Objective
The purpose of this study was to evaluate the efficacy of an adjuvanted modified live virus (MLV) vaccine in the presence of well-defined maternal passive immunity.
Study Description
Calves were vaccinated at approximately 1 month of age and challenged ~90 days later when BRSV systemic antibodies were less than 1:4. Clinical signs, nasal secretions and blood samples for virus measurement [polymerase chain reaction (PCR) and virus isolation (VI)] and to measure for mucosal BRSV IgA antibodies were collected and the animals were euthanized and necropsied 8 days post infection. Body temperature and other clinical signs were lower at 6 …
Interactions Of Change In Nutrition After Ai On Plasma Metabolites, Steroid Hormone Production, And Uterine Environment,
2021
South Dakota State University
Interactions Of Change In Nutrition After Ai On Plasma Metabolites, Steroid Hormone Production, And Uterine Environment, Taylor N. Andrews, Kaitlin M. Epperson, Jerica J.J. Rich, Saulo Menegatti Zoca, Adalaide C. Kline, Lacey K. Quail, Abigail L. Zezeski, Thomas W. Geary, Julie A. Walker, George A. Perry
SDSU Beef Day 2021 Summary Publication
Objective
The objective was to evaluate the impact of nutritional changes post artificial insemination (AI) on plasma metabolites, steroid hormones, and uterine environment.
Study Description
Beef heifers (n = 43) were randomly assigned to two dietary treatment groups (High = 161.5% or Low = 77.5% of maintenance energy) for 14 d after AI (post-AI). Post-AI dietary treatments continued until uteri were flushed for embryo recovery (d 14 post-AI). Blood samples were collected on d -3, 0 (day of AI), 3, 6, 9, 12, and 14 for analysis of plasma glucose, proteins, non-esterified fatty acids (NEFAs), and cholesterol using colorimetric assays. …
Use Of Pregnancy Associated Glycoproteins To Determine Fetal Age Throughout Gestation In Cattle,
2021
South Dakota State University
Use Of Pregnancy Associated Glycoproteins To Determine Fetal Age Throughout Gestation In Cattle, Adalaide C. Kline, Kaitlin M. Epperson, Jerica J.J. Rich, Saulo Menegatti Zoca, Taylor N. Andrews, Lacey K. Quail, Jim R. Rhoades, George A. Perry
SDSU Beef Day 2021 Summary Publication
Objective
The objective of the current study was to determine if a commercially available blood pregnancy test could be modified to detect differences in pregnancy-associated glycoprotein (PAG) concentrations to indicate stage of pregnancy or fetal age in cattle.
Study Description
Previously identified pregnant females were grouped by age (pre-primiparous or multiparous). Blood samples were collected between day 27 and 190 of pregnancy (n = 176 from pre-primiparous and n = 240 from multiparous) and serum was tested in duplicate using a commercially available blood pregnancy test, IDEXX Alertys Pregnancy Test. Procedures were adapted to allow concentrations to fall within the …
The Presence Of Persistent Bovine Viral Diarrhea Virus Infection And A Novel Bosavirus In A Bison Herd,
2021
South Dakota State University
The Presence Of Persistent Bovine Viral Diarrhea Virus Infection And A Novel Bosavirus In A Bison Herd, Ben M. Hause, Julia F. Ridpath, Angela E. Pillatzki, Tom Bragg, Christopher C.L. Chase
SDSU Beef Day 2021 Summary Publication
Objective
Bovine viral diarrhea virus (BVDV) is a significant pathogen of cattle, leading to losses due to reproductive failure, respiratory disease and immune dysregulation. An investigation was conducted in an American bison (Bison bison) herd dealing with reproductive issues in 2018-2019 calving season to determine likely cause of the losses.
Prevalence Of Bovine Viral Diarrhea Virus Subspecies Among Persistently Infected Samples From Cattle In The United States,
2021
South Dakota State University
Prevalence Of Bovine Viral Diarrhea Virus Subspecies Among Persistently Infected Samples From Cattle In The United States, Jessie Vallejo, Travis Clement, Matthew Dammen, Christopher C.L. Chase
SDSU Beef Day 2021 Summary Publication
Objective:
The focus of this survey was to determine the prevalence of BVDV subspecies in the United States.
Comparison Of Winter Cow Feeding Strategies On Offspring Carcass Characteristics And Meat Quality,
2021
South Dakota State University
Comparison Of Winter Cow Feeding Strategies On Offspring Carcass Characteristics And Meat Quality, Erin Gubbels, Christopher Schauer, Don Stecher, Donny Drolc, Robin Salverson, Adele Harty, Warren Rusche, Cody Wright, Kristi Cammack, Zachary Smith, J. Kyle Grubbs, Keith Underwood, Janna Block, Kenneth Olson, Amanda Blair
SDSU Beef Day 2021 Summary Publication
Objective
The objective of this research was to investigate effects of maternal prepartum dietary energy source (forage vs. concentrate) during mid and late gestation on carcass composition, and meat quality of offspring.
Study Description
Angus-based cows from 2 sources [n = 129 from SDSU (Experiment 1) and n = 70 from North Dakota State University (Experiment 2)] were stratified by body weight and age and placed into two treatment groups at a drylot facility during mid- and late-gestation: Concentrate (dams fed a concentrate-based diet) or Forage (dams fed a forage-based diet). Calves were finished and carcass data was collected. Striploins …
Influence Of Beef Carcass Chilling Rate On Steak Case Life And Quality Traits,
2021
South Dakota State University
Influence Of Beef Carcass Chilling Rate On Steak Case Life And Quality Traits, Trevor C. Dehaan, Amanda D. Blair, Judson K. Grubbs, Keith R. Underwood
SDSU Beef Day 2021 Summary Publication
Objective
To determine if beef carcass weight influences chilling rate, pH decline, beef color, case life and tenderness of steaks from the round, loin, rib and chuck.
Metal-Organic Framework-Based Sensor For Bacterial Trehalose In Beef Products,
2021
South Dakota State University
Metal-Organic Framework-Based Sensor For Bacterial Trehalose In Beef Products, Matthew Hummel, Hongxing Jia, Ailin Guo, Abdullah Maruf, Shun Lu, Wei He, Yue Zhou, Zhengrong Gu
SDSU Beef Day 2021 Summary Publication
Objective
The objective is to detect trehalose from pathogenic bacteria using an electrochemical sensor based on alkali earth metal-organic frameworks (AE-MOFS).
