Oral Biology and Oral Pathology Commons^™

Genetic Analysis Of Hereditary Gingival Fibromatosis Associated Sos1 Missense Variants Of Uncertain Significance In Caenorhabditis Elegans, Himani Patel

Theses

Hereditary gingival fibromatosis (HGF) is a disease that can present as benign overgrowth of gingival tissue in the mouth. The overgrowth can enclose the entire mouth and teeth in severe cases or present itself in a concentrated area. Researchers have identified that mutations in the SOS1 gene can be responsible for HGF. This disease can impair basic functions related to the mouth. Eating, smiling, speaking can all be affected. Additionally, excess inflammation can cause periodontal disease because of the difficulty in maintaining proper oral health. Periodontal disease can lead to severe bone loss which can lead to complete loss of …

The Role Of Oral Microbiota In Periodontitis And Alzheimer's Disease, Noah Al-Hassan, Taha Al Hassan, Juan C. Lopez-Alvarenga, Maria Quinones, Seratna Guadarrama

Research Symposium

Background: Periodontal disease (PD), affecting 20-50% of the global population is marked by biofilm-induced inflammation in oral tissues. Chronic PD results in systemic complications such as heart disease, stroke, and Alzheimer's. The red complex microbes, Porphyromonas gingivalis and Treponema denticola, play a pivotal role, penetrating the blood-brain barrier and contributing to neurodegeneration. Alzheimer's disease (AD), an irreversible neurodegenerative disorder, is linked to abnormal protein cleavage and potentially involves microbiologic components, including gram-positive cocci. Research suggests the presence of bacteria such as Porphyromonas, Actinomyces, and Treponema in autopsied AD brains. Investigating the microbiologic connection between PD and AD is crucial, considering …

Squamous Odontogenic Tumor: A Case Report, Sara Endo Dds, Timothy Chang Dds, Daria Vasilyeva Dds, Scott M. Peters Dds

The New York State Dental Journal

Squamous odontogenic tumors (SOT) are slow-growing, benign, rare intraosseous neoplasms. SOT are typically found in the premolar-canine region of the maxilla and the molar region of the mandible. These lesions have no gender predilection and show peak occurrence in the third decade of life.

Sulfamethoxazole-Trimethoprim Induced Thrombocytopenia: A Report Of Two Cases And A Literature Review, Leigh Griffin Dds, Michael Graffeo Dds, Jay Sher Dds, Paul D. Freedman Dds, Renée F. Reich

The New York State Dental Journal

Sulfamethoxazole- trimethoprim (ST) is a sulfonamide derivative antibiotic that interferes with bacterial folic acid synthesis and growth by blocking dihydrofolic acid formation from para-aminobenzoic acid. One of the potential side effects of sulfamethoxazole-trimethoprim is thrombocytopenia. Thrombocytopenia is a blood disorder characterized by a decreased number of circulating platelets. We present two cases of ST induced thrombocytopenia where the oral manifestations were the first indication of the low platelet count leading to life saving interventions. Our aim is to educate dentists about the oral manifestations of thrombocytopenia and its association with ST as their examination may be instrumental in directing treatment.

Shed Stem Cells: Antibacterial And Immunomodulatory Insights, Manju R Prof

Annual Research Symposium

SHED- Stem cells extracted from exfoliated teeth - resource for Stem Cell based regenerative therapy; multipotent in nature

We examined explored how SHED affects the growth of common oral bacteria, Streptococcus mutans and Enterococcus faecalis, and their role in regulating immune response.

Non-Hodgkin's Lymphoma Of The Oral Cavity - A Rare Entity, Anita J. Florence Dr, Karpagaselvi Sanjai Prof & Dr

Annual Research Symposium

Non-Hodgkin’s lymphoma (NHL) is a subtype of lymphoma with two-thirds of the cases presenting as lymph node enlargement. The remaining one third of NHL cases has been reported in the extra nodal sites, including the gastro-intestinal tract, Waldeyer’s ring, bone, skin and brain. Intraoral NHL is uncommon and may affect either the jaw bones or occur within the soft tissues of the oral cavity. Here we report an unusual case of non-Hodgkin’s lymphoma in a 54-year-old male patient who presented with a growth from the right lower molar extraction socket with pus discharge.

Automated Micronuclei Detection In Exfoliated Oral Epithelial Cells Of Smokers Using Image Analysing Softwares, Mukul Saini, Sowmya Sv, Dominic Augustine, Sanjana Shetty, Saiprasad Alva

Annual Research Symposium

Micronuclei assay yields an excellent path to monitor individuals or populations exposed to mutagenic, genotoxic or teratogenic events. Micronuclei are small nucleic structures formed due to the deposition of nuclear envelopes around lagging chromosomes that persist in the interphase. Mutagenic and carcinogenic compounds, such as polycyclic aromatic hydrocarbons and N-nitrosamines are believed to be responsible for the formation of micronuclei. Hence, micronuclei detection in exfoliated oral epithelial cells of smokers is a significant biomarker for genotoxicity and to identify cellular changes of biological importance to carcinogenesis. While studies have been conducted for the detection of micronuclei in smokers, they may …

Application Of In-Silico Computational Biology Strategies To Demonstrate Mechanism Of Oral Cancer Cell Death By Natural Peptide, Dr Dominic Augustine

Annual Research Symposium

This research work is designed to identify biomolecules from Earthworm Coelomic Fluid (ECF) of Eudrilus Eugeniae (EE) that can inhibit oral cancer cells from proliferating. The study aims to construct a homology model of the 18 kDa protein from ECF of EE (18-ECFP) with molecular dynamics simulation (MDS) to enable its molecular docking with pro-apoptotic caspase receptors with determination of binding energy scores. The study also evaluates the anti-cancer potential of 18-ECFP on SCC-9 cells in vitro by wet lab techniques.

Pathogenic Implications Of Candidal Species In Both Oral Cancer And Oral Potentially Malignant Disorders - A Review, Dr Dominic Augustine, Sowmya Sv, Kuntala Mondal

Annual Research Symposium

Oral squamous cell carcinoma (OSCC) is the 6th most common cancer in worldwide. Cigarette smoking, alcohol consumption, and betel quid chewing are its major risk factors other factors that can lead to OSCC include excessive sun exposure, viral or fungal infection, poor nutrition, and poor oral hygiene. The carcinogenesis of OSCC occurs gradually and oral epithelial dysplasia is considered a potential histologic precursor of OSCC. Most OSCC cases are preceded by clinically evident oral potentially malignant disorders (OPMDs). The aim of this review is to correlate the association between Candida species and oral potentially malignant disorders and its possible mechanisms …

Immunohistochemical Expression Of Cyclin D1 In Dentigerous Cyst, Odontogenic Keratocyst And Unicystic Ameloblastoma- An In-Vitro Study, Kuntala Mondal, Sv Sowmya, Dominic Augustine

Annual Research Symposium

Cyclin D1, a G1 cyclin member, regulates the transition of the cell cycle from the G1 phase to the S phase. The uncontrolled and elevated expression of cyclin D1 has been associated with rapid growth, heightened proliferative activity and increased histologic aggressiveness. Unicystic ameloblastoma pertains to cystic lesions that present clinical, radiographic, or gross features akin to jaw cysts. Nevertheless, upon histological scrutiny, these lesions display a distinctive ameloblastomatous epithelium lining the cystic cavity, with or without luminal or mural proliferation. This cystic variant of tumor bears substantial resemblances to dentigerous cysts, and occasionally, both entities can become indistinguishable upon …

An Immunohistochemical Panel For The Accurate Differentiation Between Mucoepidermoid Carcinoma And Pleomorphic Adenoma, Kuntala Mondal, Sv Sowmya, Dominic Augustine

Annual Research Symposium

Salivary Gland Tumors (SGTs) are a diverse group of neoplasms, with a wide range of histological subtypes, some of which can exhibit overlapping features. Amongst this group of tumors, Pleomorphic Adenoma (PA) and Mucoepidermoid Carcinoma (MEC) stand out as prevalent entities originating from the salivary glands. The accurate differentiation between these two entities is crucial for treatment planning, as it influences decisions regarding the extent of surgical resection. Because of the overlapping histopathological features, it is difficult to differentiate these two lesions with the help of conventional Hematoxylin & Eosin (H& E). The evaluation of Immunohistochemical (IHC) markers holds pivotal …

Cheiloscopic Characteristics Detection And Pattern Classification By Machine Learning Technique, Dr Dominic Augustine, Sowmya Sv

Annual Research Symposium

Machine Learning (ML) is a field enhancing the rapid growth of technology. Though use of digital softwares for cheiloscopic investigations have been attempted with limited success, the use of ML based techniques are scarce and seldom have been employed in forensic odontology. The present study aimed to identify cheiloscopic patterns through machine learning based methods and to correlate the segmented patterns with age and gender of individuals.

Association Between Abo Blood Grouping And Odontometry: An Unexplored Evidence In Forensics, Dr Dominic Augustine, Sowmya Sv

Annual Research Symposium

Recent research has exhibited a strong association between ABO blood groups and susceptibility to diseases. Extraoral and intraoral hard tissue landmarks provide a stable and reliable record for odontological and anthropological analysis, forensic investigations and identification. The association between anthropometric indices and ABO blood grouping have been reported in the past, however, the association between ABO blood groups and odontometric indices is yet to be comprehensively explored. This study aimed to establish the correlation of ABO blood groups with odontometric indices and extraoral hard tissue landmarks for personnel identification.

Advanced Techniques For Tooth Pulp Rna Extraction In Forensic Investigations-A Systematic Review, Kuntala Mondal, Sv Sowmya, Dominic Augustine

Annual Research Symposium

Teeth are fundamental structures in a forensic context due to their high strength and specificity. It is challenging to obtain high quality RNA from pulpal tissues. It has been described that pulp RNA degradation could be an indicator of post-mortem interval. Researchers have been in the quest to develop optimal techniques for tooth RNA extraction. The systematic review aimed to determine the most reliable method of RNA extraction from dental tooth pulp for forensic investigations.

Assessment Of Micrometastasis In Lymph Nodes Of Oral Squamous Cell Carcinoma –Attaining Excellence Through Immunohistochemistry, Dr Dominic Augustine, Sowmya Sv, Kuntala Mondal

Annual Research Symposium

Cervical lymph node metastasis is the most important prognostic factor in Squamous Cell Carcinoma of Head and Neck (SCCHN). Lymph node prognostic determinants hold immense significance in influencing both patient’s survival outcomes and the propensity for recurrence. The current study aimed to evaluate the diagnostic accuracy of Cyclin D1 and Pan-CK for the detection of lymph node micrometastasis in oral squamous cell carcinoma tissue sections and re-evaluate the tumor staging.

Clinical, Radiographic And Biochemical Evaluation Of One-Piece Vs Two-Piece Single Implants After 5 Years Of Functional Loading, Ippolito Caputo

Annual Research Symposium

The aim of this study was to compare the clinical and radiographic conditions and the expression of proinflammatory cytokines in peri-implant crevicular fluid (PICF) of two-piece/bone level (TP/BL) versus one-piece/tissue level (OP/TL) single implants with a laser-microgrooved collar after at least 5 years of functional loading.

Detection Of Cervical Lymph Node Micrometastasis - A Machine Learning Based Approach, Dr. Sowmya Sv, Dominic Augustine, Kuntala Mondal

Annual Research Symposium

No abstract provided.

Matlab Based Phenotypic Evaluation And Gene Expression Of Cd44 And Cd133 Biomarkers In The Prediction Of Oral Squamous Cell Carcinoma Metastasis, Dr. Sowmya Sv

Annual Research Symposium

Oral cancer accounts to 20–30% of all cancers in India. The major difficulty in managing carcinomas is due to failure in the control and detection of metastases (>90% deaths). Cancer stem cells (CSCs) are tumor-initiating cells that survive even after extensive radiotherapy and chemotherapy. Recently, the use of computer-assisted quantification methods employing image processing is drawing attention wherein information can be automatically extracted from the immunostained digitized images. The aim of the study was to assess the metastatic risk of Oral Squamous Cell Carcinoma (OSCC) by the phenotypic evaluation and gene expression of CD44 and CD133 biomarkers with clinical …

Determination Of The Correlation Between Age And Gender With Canine Index, Intermolar Width And Palatal Rugae Patterns, Dr. Sowmya Sv, Dominic Augustine

Annual Research Symposium

Dental records are of paramount importance in human identification considering the distinct traits of every individual. Although DNA and finger print analysis are techniques employed for faster and secure identification, in mass disasters, odontometric analysis can play a vital role in identification. Studies in the past have used parameters like arch perimeters, lip prints and palatal rugae pattern to determine age and gender of an individual. However, these studies correlated one parameter at a time and concluded that there was no significant correlation of the parameters to age and gender. The present study aimed to assess the canine index, intermolar …

Convolutional Neural Network Based Analysis - An Aid To Diagnose Bacterial And Fungal Osteomyelitis, Saiprasad Alva, Dominic Augustine, Sowmya Sv, Mukul Saini, Sanjana Shetty

Annual Research Symposium

Osteomyelitis may be classified as Bacterial (Actinomycotic), Fungal (Mucormycotic), or combined based on the etiological agent. During histopathological examination, there is a high chance that bacterial colonies or fungal hyphae may be missed by the human eye, especially when there is a paucity of organisms. This may lead to a faulty diagnosis of the type of osteomyelitis which along with an improper treatment plan would cause further progression of the disease and various other complications. Therefore, the diagnosis of the exact etiological variant of osteomyelitis is of prime importance to design an appropriate treatment plan. In the present study, bone …