Biological and Chemical Physics Commons^™

Pd Doped Sno2 Resistive Film As A Non-Enzymatic Glucose Sensor, Salim F. Bamsaoud, Sandhya Rani, R. N. Karekar Prof., S. W. Gosavi

Hadhramout University Journal of Natural & Applied Sciences

A new sugar sensor based on metal oxide is presented here. The variation on the sensor resistance is studied against different glucose and fructose concentrations (1 - 360 mg/dl). The study is done for three different resistive films (i) pristine palladium oxide (PdOx); (ii) tin oxide (SnO2) and (iii) palladium doped tin oxide (Pd-SnO2). The chemical reactions of glucose and fructose with surface species were studied. Pd-SnO2 films show an excellent and reliable response to glucose and fructose solution in comparison with the bare films (PdOx and SnO2). Sensor performance was checked for the blood of normal (120 mg/d) and …

Hobby Grade Lithium-Ion Batteries For Spacecraft Applications: Establishing An Automated Electrical Characteristics Testing Procedure For Flight Acceptance Of Non-Space-Grade Small Secondary Batteries, Braidon Hughes

Graduate Theses and Dissertations

Li-ion batteries are widely used due to the large amount of rechargeable energy they pack into a small, light package. This higher energy density makes Li-ion batteries ideal for small satellite applications, specifically CubeSats. CubeSats have grown in popularity in higher level education due to the National Aeronautics and Space Administration’s implementation of the Cube Satellite Launch Initiative, making it easier and cheaper to conduct small, low orbit missions. Because these CubeSats are occupying the same space as a crewed spacecraft, it is imperative that they are safe. There are numerous reports of Li-ion batteries creating fires that result in …

Investigation Of Optical And Structural Properties Of Gesn Heterostructures, Oluwatobi Gabriel Olorunsola

Graduate Theses and Dissertations

Silicon (Si)-based optoelectronics have gained traction due to its primed versatility at developing light-based technologies. Si, however, features indirect bandgap characteristics and suffers relegated optical properties compared to its III-V counterparts. III-Vs have also been hybridized to Si platforms but the resulting technologies are expensive and incompatible with standard complementary-metal-oxide-semiconductor processes. Germanium (Ge), on the other hand, have been engineered to behave like direct bandgap material through tensile strain interventions but are well short of attaining extensive wavelength coverage. To create a competitive material that evades these challenges, transitional amounts of Sn can be incorporated into Ge matrix to form …

Analysis Of Bacteriorhodopsin Suspended In A Bilayer Lipid Membrane, Moath Alhejji

Graduate Theses and Dissertations

The bacteriorhodopsin protein’s unique characteristic of proton pumping can convert light energy to electric energy. The aim of this research was to generate photocurrent using bacteriorhodopsin in a bi-layer lipid membrane. Lipid monolayer and bilayer were formed using painting and folding methods, respectively. Capacitance and resistance of the lipid membranes were measured and used to validate the best methodology. My results show that the folding method is more efficient in incorporating Bacteriorhodopsin. The photocurrent was generated by illuminating a green laser (532 nm) on the bilayer lipid membranes. The patch clamp electrophysiology technique was used to apply voltage across the …

A Neutral Model Of 100 Million Years Of Chromosome Inversions In The Yeast Genus Lachancea, Brian Clark

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Reinvigorating A Technical Countering Weapons Of Mass Destruction Distance Learning Graduate Certificate Program, James C. Petrosky, Gaiven Varshney, Jeremy Slagley, Sara Shaghaghi

Faculty Publications

Current Countering Weapons of Mass Destruction (CWMD) demands can be divided broadly into policy and science. The science of chemical, biological, and radiological/nuclear weapons informs the limits of development, production, employment, operation, detection, risk characterization, human and material protection, and medical intervention. In short, the science of weapons of mass destruction (WMD) should precede and inform the development of policy. It is to this end that the Air Force Institute of Technology (AFIT) CWMD program was re-established, providing a technical educational option for practitioners to understand the science behind a very technically challenging subject.

Critical Behavior In Evolutionary And Population Dynamics, Stephen Ordway

Dissertations

This study is an exploration of phase transition behavior in evolutionary and population dynamics, and techniques for predicting population changes, across the disciplines of physics, biology, and computer science. Under the looming threat of climate change, it is imperative to understand the dynamics of populations under environmental stress and to identify early warning signals of population decline. These issues are explored here in (1) a computational model of evolutionary dynamics, (2) an experimental system of decaying populations under environmental stress, and (3) a machine learning approach to predict population changes based on environmental factors. Through the lens of critical phase …

Molecular Dynamics Simulations Of Self-Assemblies In Nature And Nanotechnology, Phu Khanh Tang

Dissertations, Theses, and Capstone Projects

Nature usually divides complex systems into smaller building blocks specializing in a few tasks since one entity cannot achieve everything. Therefore, self-assembly is a robust tool exploited by Nature to build hierarchical systems that accomplish unique functions. The cell membrane distinguishes itself as an example of Nature’s self-assembly, defining and protecting the cell. By mimicking Nature’s designs using synthetically designed self-assemblies, researchers with advanced nanotechnological comprehension can manipulate these synthetic self-assemblies to improve many aspects of modern medicine and materials science. Understanding the competing underlying molecular interactions in self-assembly is always of interest to the academic scientific community and industry. …

Monte Carlo, Molecular Dynamics And Network Analysis Of The Gramicidin Water Channel And Proton Transfer Pathways To Qb In Photosynthetic Reaction Centers, Yingying Zhang

Dissertations, Theses, and Capstone Projects

Water molecules play a key role in all biochemical processes. They help define the shape of proteins, and they are reactant or product in many reactions and are released as ligands are bound. They facilitate transfer of protons through transmembrane proton channel, pump and transporter proteins. Continuum electrostatics (CE) force fields such as used in MCCE (Multi-Conformation Continuum Electrostatics) capture electrostatic interactions in biomolecules with an implicit solvent, to give the averaged solvent water equilibrium properties. Hybrid CE methods can use explicit water molecules within the protein surrounded by implicit solvent. These hybrid methods permit the study of explicit hydrogen …

Therapeautic Cerium Oxide Nanoparticles, Angel E. Vasquez

Symposium of Student Scholars

The overall goal of the research project is to create a glass that produces cerium oxide nanoparticles and as an efficient delivery mechanism. Cerium is able to exist as Ce3+ and Ce4+ because it has two partially filled subshells. This coexistence allows cerium oxide to have antioxidant properties that reduce the number of free radicals in that body that are associated with cancer, diabetes, and neurodegenerative diseases. In our laboratory, using a soluble borate glass, cerium oxide nanoparticles are created to coexist in Ce3+ and Ce4+ valences This borate glass composition is doped with different amounts of Cerium(IV) Oxide and …

Thermal Properties Of 18f-Fdg Uptake And Imaging In Positron Emission Tomography Scans Of Cancerous Cells, Carleigh R. Eagle

PANDION: The Osprey Journal of Research and Ideas

Positron Emission Tomography (PET) scans can utilize a radioactive tracer, in this case 2-deoxy2-[fluorine-18] fluoro-D-glucose (¹⁸F-FDG), to visualize malignant tumors in cancer patients. The uptake was compared to glucose to understand the difference in thermal properties, which contribute to the ability to image the cancerous cells. The uptake of ¹⁸F-FDG by cancer cells and the imaging process of positron emission tomography were reviewed from a thermodynamic perspective. Gastrointestinal and neurological imaging techniques were reviewed to understand the role of PET imaging in different areas of the human body.

Advancement Of A 3d Computational Phantom And Its Age Scaling Methodologies For Retrospective Dose Reconstruction Studies, Aashish Gupta

Dissertations and Theses (Open Access)

We have used a 3D age-scalable computational phantom for over two decades for retrospective dose reconstruction studies of childhood cancer survivors (CCS) treated with 2D historic radiotherapy (RT). However, our phantom and its age scaling functions (ASF) must be updated so that it can be used in studies that include survivors treated with contemporary RT. We aimed to implement our phantom and its age scaling functions in DICOM format and determine the feasibility of applying our ASFs to accurately scale the whole-body CT-based anatomies.

In the implementation study, we developed Python scripts that model the phantom and ASFs in a …

Sustainable And Biodegradable Nanocellulose Composite Materials, Jacob Scheel, Tim Kidd Ph.D.

Summer Undergraduate Research Program (SURP) Symposium

Nanocellulose is made from a mixture of cellulose powder, and a variety of different materials and liquids. Unfortunately the cost to produce nanocellulose isn’t ideal, thanks to shrinkage. The goal of my research this year was to try to make nanocellulose more cost effective, by reduce shrinkage.

Bridging The 12-6-4 Model And The Fluctuating Charge Model, Pengfei Li

Chemistry: Faculty Publications and Other Works

Metal ions play important roles in various biological systems. Molecular dynamics (MD) using classical force field has become a popular research tool to study biological systems at the atomic level. However, meaningful MD simulations require reliable models and parameters. Previously we showed that the 12-6 Lennard-Jones nonbonded model for ions could not reproduce the experimental hydration free energy (HFE) and ion-oxygen distance (IOD) values simultaneously when ion has a charge of +2 or higher. We discussed that this deficiency arises from the overlook of the ion-induced dipole interaction in the 12-6 model, and this term is proportional to 1/r …

Awegnn: Auto-Parametrized Weighted Element-Specific Graph Neural Networks For Molecules., Timothy Szocinski, Duc Duy Nguyen, Guo-Wei Wei

Mathematics Faculty Publications

While automated feature extraction has had tremendous success in many deep learning algorithms for image analysis and natural language processing, it does not work well for data involving complex internal structures, such as molecules. Data representations via advanced mathematics, including algebraic topology, differential geometry, and graph theory, have demonstrated superiority in a variety of biomolecular applications, however, their performance is often dependent on manual parametrization. This work introduces the auto-parametrized weighted element-specific graph neural network, dubbed AweGNN, to overcome the obstacle of this tedious parametrization process while also being a suitable technique for automated feature extraction on these internally complex …

Biochemical Impact Of Solar Radiation Exposure On Human Keratinocytes Monitored By Raman Spectroscopy; Effects Of Cell Culture Environment, Ulises Lopez Gonzalez, Alan Casey, Hugh Byrne

Articles

Understanding and amelioration of the effects of solar radiation exposure are critical in preventing the occurrence of skin cancer. Towards this end, many studies have been conducted in 2D cell culture models under simplified and unrealistic conditions. 3D culture models better capture the complexity of in vivo physiology, although the effects of the 3D extracellular matrix have not been well studied. Monitoring the instantaneous and resultant cellular responses to exposure, and the influence of the 3D environment, could provide an enhanced understanding of the fundamental processes of photocarcinogenesis. This work presents an analysis of the biochemical impacts of simulated solar …

Somatic Inhibition By Microscopic Magnetic Stimulation, Hui Ye

Biology: Faculty Publications and Other Works

Electric currents can produce quick, reversible control of neural activity. Externally applied electric currents have been used in inhibiting certain ganglion cells in clinical practices. Via electromagnetic induction, a miniature-sized magnetic coil could provide focal stimulation to the ganglion neurons. Here we report that high-frequency stimulation with the miniature coil could reversibly block ganglion cell activity in marine mollusk Aplysia californica, regardless the firing frequency of the neurons, or concentration of potassium ions around the ganglion neurons. Presence of the ganglion sheath has minimal impact on the inhibitory effects of the coil. The inhibitory effect was local to the …

Recovery Of High Specific Activity Molybdenum-99 From Accelerator-Induced Fission On Low-Enriched Uranium For Technetium-99m Generators, M. Alex Brown, Nathan Johnson, Artem V. Gelis, Milan Stika, Anna G. Servis, Alex Bakken, Christine Krizmanich, Kristin Shannon, Peter Kozak, Amanda Barnhart, Chad Denbrock, Nicolas Luciani, Terry Grimm, Peter Tkac

Chemistry and Biochemistry Faculty Research

A new process was developed to recover high specific activity (no carrier added) 99Mo from electron-accelerator irradiated U3O8 or uranyl sulfate targets. The process leverages a novel solvent extraction scheme to recover Mo using di(2-ethylhexyl) phosphoric acid following uranium and transuranics removal with tri-n-butyl phosphate. An anion-exchange concentration column step provides a final purification, generating pure 99Mo intended for making 99Mo/99mTc generators. The process was demonstrated with irradiated uranium targets resulting in more than 95% 99Mo recovery and without presence of fission products or actinides in the product.

The Early Medieval Transition: Diet Reconstruction, Mobility, And Culture Contact In The Ravenna Countryside, Northern Italy, Anastasia Temkina

USF Tampa Graduate Theses and Dissertations

This research project evaluates the effects of increased mobility and culture contact on dietary practices, and on dietary variation among people buried at two northern Italian sites, Chiunsano di Ficarolo and Chiesazza di Ficarolo, located near the ancient Roman capital of Ravenna and dating 4th to 7th century CE. The Early Medieval period was a time of change, political instability, migration and invasion of the “barbarian” tribes, and diet was not unaffected. In particular, it is hypothesized that a new staple crop, millet, was introduced and that meat consumption had increased. The goal of this research is to use stable …

Protein Motifs For Proton Transfers That Build The Transmembrane Proton Gradient, Divya Kaur, Umesh Khaniya, Yingying Zhang, M. R. Gunner

Publications and Research

Biological membranes are barriers to polar molecules, so membrane embedded proteins control the transfers between cellular compartments. Protein controlled transport moves substrates and activates cellular signaling cascades. In addition, the electrochemical gradient across mitochondrial, bacterial and chloroplast membranes, is a key source of stored cellular energy. This is generated by electron, proton and ion transfers through proteins. The gradient is used to fuel ATP synthesis and to drive active transport. Here the mechanisms by which protons move into the buried active sites of Photosystem II (PSII), bacterial RCs (bRCs) and through the proton pumps, Bacteriorhodopsin (bR), Complex I and Cytochrome …

Electrochemical And Spectroscopic Characterization Of Cerium Salts And Nanoceria Material, Emily Velarde, Wei Zhou

Symposium of Student Scholars

The nanoscale form of cerium oxide, nanoceria (nano-CeO_x), has drawn great attention in recent years in electrochemical, nanomaterial research and medicinal studies due to its antibacterial properties, UV absorption, and its biochemical function as possible radical scavenger. Many new synthesis methods have achieved uniform and biocompatible nanoceria particles, and our lab has created cerium oxide particles that shows UV/Vis. absorption and X-ray patterns similar to the commercial nanoceria and nanoceria made in other research laboratories with novel synthetic methods.

This study focuses on charactering and comparing electrochemical properties of cerium inorganic salts and synthesized nanoceria. Preliminary results …

Photochemistry Of Nh2No2 And Implications For Chemistry In The Atmosphere, Vincent J. Esposito, Tarek Trabelsi, Joseph S. Francisco

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Recent studies have indicated that nitramide (NH₂NO₂) may be formed more plentifully in the atmosphere than previously thought, while also being a missing source of the greenhouse gas nitrous oxide (N₂O) via catalyzed isomerization. To validate the importance of NH₂NO₂ in the Earth’s atmosphere, the ground and first electronic excited states of NH₂NO₂ were characterized and its photochemistry was investigated using multireference and coupled cluster methods. NH₂NO₂ is non-planar and of singlet multiplicity in the ground state while exhibiting large out-of-plane rotation in the triplet …

Raman Spectroscopic Characterisation Of Non Stimulated And Stimulated Human Whole Saliva, Genecy Calado, Isha Behl, Hugh Byrne, Fiona Lyng

Articles

Human saliva is a unique biofluid which can reflect the physiopathological state of an individual. The wide spectrum of molecules present in saliva, compounded by the close association of salivary composition to serum metabolites, can provide valuable information for clinical diagnostic applications through highly sensitive vibrational spectroscopic techniques such as Raman spectroscopy. However, the nature of saliva, in terms of collection and patient-related characteristics, can be considered factors which may strongly affect the Raman spectral profile of salivary samples and disrupt the search for specific salivary biomarkers in the detection of diseases. The main objective of this study was to …

Abhd5 Induced Morphological Changes On Model Membrane Systems, Nasser S. Junedi

Honors College Theses

Proper regulation of neutral lipid storage (lipogenesis) and release (lipolysis) are critical molecular processes localized to an organelle called the Lipid Droplet (LD). The LD consists of a core with neutral lipids such as triacylglycerols (TAGs) and sterol esters surrounded by a phospholipid monolayer. Dysregulation of the processes localized to the LD are involved in the pathology of various diseases such as Neutral Lipid Storage Disease, diabetes, stroke and cancer. The non-enzymatic protein ABHD5 (α-β Hydrolase Domain-Containing Protein 5), is thought to play a key role in the process of lipolysis by forming homo-oligomers on the surface of the LD …

Identifying Skeletal Puberty Stages In A Modern Sample From The United States, Jordan T. Wright

USF Tampa Graduate Theses and Dissertations

Investigating the onset and progression of puberty can provide insight and evidence for social changes regarding the transition to adulthood. There are also many social factors that can lead to variation in the timing of the onset and progression of puberty. Methods created by Mary Lewis and Fiona Shapland for assessing changes in skeletal development associated with pubertal timing were applied to computerized tomography scans (n=400) from the New Mexico Decedent Image Database and recorded in an attempt to identify adolescent growth trends in a modern skeletal sample from the United States. This is a novel study using the methods …

Pregnancy And Fertility Amongst Women With The Mthfr C677t Polymorphism: An Anthropological Review, Caroline A. Maclean

USF Tampa Graduate Theses and Dissertations

Effects of the MTHFR C677T allele on reproduction include multiple complications. The frequency of births with neural tube defects, Down Syndrome, or recurrent pregnancy loss of CC, CT, and TT mothers was measured using data collected from existing literature. Total participants n=2605 (n=1111 cases, n=1494 controls). Results show that folic acid supplementation did not prevent the incidence of NTD pregnancy in CT women. Furthermore, CT women are more fecund overall, producing more pregnancies than CC and TT women. While CT case women did have higher incidences of RPL, NTD pregnancy and DS pregnancy, this is due to the overall greater …

Listening To Women: Using A Mixed-Methods Approach To Understanding Women’S Desires And Experience During Childbirth, Nicole Loraine Falk Smith

USF Tampa Graduate Theses and Dissertations

This dissertation is based on evolutionary medicine and applied anthropological research where the narratives and biology of modern women are used to inform evolutionary theory, specifically obligate midwifery, and modern birth practices. Improvements to the US maternity care systems are needed as rates of maternal mortality and morbidity are continually higher than rates in other technologically advanced nations. Additionally, women birthing under the technocratic model of care, as is predominant in the US, report trauma and post-traumatic stress disorders due to their birthing experience, and more women are experiencing postpartum depression. Thus, I seek to use an evolutionary medicine perspective …

Remotely Controlled Enzyme Behavior Using Localized Thermal Gradients, Sarah Brown

UNO Student Research and Creative Activity Fair

No existing model describes an identified special case of enzyme behavior when directly stimulated with thermal energy via an RF field.

The ability to remotely influence cellular functions and outcomes is a new approach to targeted medicine. Nano-medicine and biotechnology are the future of medical diagnostics and treatment. The ability to remotely influence cellular functions and outcomes is a new approach to targeted medicine. Direct heating of an enzyme vs bulk heating changes the enzyme activity.

I tested how direct transfer of thermal energy changes rates of enzyme reactions. We created samples of enzymes, attached to ferrous (magnetic) nano-particles, and …

Alternative View Of Oxygen Reduction On Porous Carbon Electrocatalysts: The Substance Of Complex Oxygen-Surface Interactions, Giacomo De Falco, Marc Florent, Jacek Jagiello, Yongqiang Cheng, Luke L. Daemen, Anibal J. Ramirez-Cuesta, Teresa J. Bandosz

Publications and Research

Electrochemical oxygen reduction reaction (ORR) is an important energy-related process requiring alternative catalysts to expensive platinum-based ones. Although recently some advancements in carbon catalysts have been reported, there is still a lack of understanding which surface features might enhance their efficiency for ORR. Through a detailed study of oxygen adsorption on carbon molecular sieves and using inelastic neutron scattering, we demonstrated here that the extent of oxygen adsorption/interactions with surface is an important parameter affecting ORR. It was found that both the strength of O2 physical adsorption in small pores and its specific interactions with surface ether functionalities in the …

Diagnostics Of A Large Volume Pin-To-Plate Atmospheric Plasma Source For The Study Of Plasma Species Interactions With Cancer Cell Cultures, Laurence Scally, Chaitanya Sarangapani, Brijesh Tiwari, Renee Malone, Hugh Byrne, James Curtin, P.J. Cullen

Articles

A large gap pin-to-plate, atmospheric pressure plasma reactor is demonstrated as means of in vitro study of plasma species interactions with cell cultures. By employing optical emission and optical absorption spectroscopy, we report that the pin-to-pate plasma array had an optimal discharge frequency for cell death of 1000 Hz in ambient air for the target cancer cell line; human glioblastoma multiform (U-251MG). The detected plasma chemistry contained reactive oxygen and nitrogen species including OH, N₂, N₂⁺, and O₃. We show that, by varying the plasma discharge frequency, the plasma chemistry can be tailored …