Pharmacology Commons^™

High-Performance Liquid Chromatographic Determination Of Asiatic Acid In Human Plasma, Phensri Thongnopnua

The Thai Journal of Pharmaceutical Sciences

Asiatic acid, an active metabolite of asiaticoside, is one of the biological active triterpenoids in Centella asiatica. A single liquid-liquid extraction and high-performance liquid chromatographic (HPLC) method have been developed and validated for the ...

Spectrophotometric Determination Of Total Alkaloids In Some Iranian Medicinal Plants, Fazel Shamsa, Hamidreza Monsef, Rouhollah Ghamooshi, Mohammadreza Verdian-Rizi

The Thai Journal of Pharmaceutical Sciences

A simple spectrophotometric method based on the reaction with bromocresol green (BCG) has been developed for determination of total alkaloids in medicinal plants. A yellow complex forms and is easily extractable by chloroform at pH 4.7. The absorbance of...

Antimicrobial Activity Of Endophytic Bacteria Isolated From Thai Medicinal Plant(ฤทธิ์ต้านจุลินทรีย์ของแบคทีเรียเอนโดไฟท์ที่แยกจากสมุนไพรไ..., Areerat Laorpaksa, Suree Jianmongkol, Wimon Pothiwong

The Thai Journal of Pharmaceutical Sciences

Endophytic bacteria were isolated from internal plant tissues within four days of collections using a surface disinfected method. One hundred and six isolates from thirty five of Thai medicinal plants were cultured in either nutrient broth or nutrient br...

Mixing And Formulation Of Low Dose Drugs : Underlying Problems And Solutions, Vipin Kukkar, Vikas Anand, Mahesh Kataria, Manoj Gera, Pratim Kumar Choudhury

The Thai Journal of Pharmaceutical Sciences

Mixing and formulation of low dose drugs can be very challenging due to problems related to segregation, content uniformity and physical stability. A careful control on these factors is necessary while manufacturing low dose drug products. Selection of e...

The Metastasis Suppressor Nm23-H1 Is Required For Dna Repair, Mengmeng Yang

University of Kentucky Doctoral Dissertations

NM23-H1 represents the first identified metastasis suppressor, exhibiting reduced expression in breast carcinoma and melanoma, and an ability to inhibit metastatic growth without significant impact on the transformed phenotype. Although its molecular mechanism of action is not fully understood, NM23-H1 possesses at least three enzymatic activities that may mediate metastasis suppressor function. It catalyzes nucleoside diphosphate kinase (NDPK) activity, as well as protein histidine kinase and 3’-5’ exonuclease activities. As 3’-5’ exonucleases are generally required for maintenance of genomic integrity, this activity represents a plausible mediator to underlie the metastasis suppressor function of NM23-H1 protein. To investigate the relevant activity …

Layer -By -Layer Self -Assembly Of Micro-Capsules For The Magnetic Activation Of Semi-Permeable Nano-Shells, Malcolm D. Prouty

Doctoral Dissertations

Layer-by-layer (LbL) self-assembly has demonstrated broad perspectives for encapsulating, and the controllable delivery, of drugs. The nano-scale polymer layers have the capability of material protection. Magnetic nanoparticles have great potential to be applied with LbL technology to achieve both "focusing" of the encapsulated drugs to a specific location followed by "switching" them on to release the encapsulated drugs. In this work, Phor21-βCG(ala), dextran, and dexamethasone were used as model drugs. Encapsulation of these drugs with layer-by-layer self-assembly formed biolnano robotic capsules for controlled delivery and drug release.

Silica nanoparticles coated with polyelectrolyte layers of sodium carboxymethyl cellulose (CMC) or gelatin …

Maturational Changes In Cerebrovascular Thick- And Thin-Filament Regulation, Renan Joel Sandoval

Loma Linda University Electronic Theses, Dissertations & Projects

The present study focused on the main hypothesis that age-related changes in myofilament Ca²⁺ sensitivity involve simultaneous changes in the relations between cytosolic Ca²⁺ transients, myosin phosphorylation, and force development. To define the effects of late fetal and early postnatal maturation, parallel experiments were carried out using cerebral arteries from both term lamb fetuses (140-141 days of gestation) and nonpregnant adult sheep. Fetal and adult cerebral arteries were stimulated by either mechanically, electromechanically, and/or pharmacomechanically-induced contractions, and the relations of cytosolic Ca²⁺, MLC phosphorylation, and contractile tensions were measured. These measurements allowed for stimulus and age-dependent …

Regulation Of Alpha-1-Adrenoceptor-Mediated Contractions Of The Uterine Artery By Pkc, Hongying Zhang

Loma Linda University Electronic Theses, Dissertations & Projects

Pregnancy is associated with a significant decrease in uterine vascular tone and a striking increase in uterine blood flow, which ensures normal fetal development. Multiple mechanisms are involved in the adaptation of uterine artery contractility during pregnancy. Both protein kinase C (PKC) and activation of ai-adrenoceptors play important roles in the regulation of uterine artery contractility. In addition, PKC mediates the pregnancyassociated decrease in myogenic tone of the uterine artery, and interacts with ajadrenoceptors leading to modulation of ai-adrenoceptor-mediated contractions of vascular smooth muscle. Yet, whether or to what extent PKC regulates ai-adrenoceptormediated contractions of the uterine artery and their …

Immunoadjuvanticity Of Chlorophyll, Jocelyn Helen Parks Ramos

All-Inclusive List of Electronic Theses and Dissertations

Background: Vaccines are formulations containing antigenic determinants (from non-self organic matters) and immuno-enhancers called adjuvants. Together, they evoke protective immune responses in hosts. Adjuvants are substances that further vaccines' capability to induce a robust immune response. Common side effects with conventional adjuvants used in most vaccines are toxicity, inflammation and granulomas. In this study we investigated the pigment chlorophyll (Chl) for immunoadjuvanticity. The selection of chlorophyll is based on the fact that it is a component in foods such as spinach, and that it contains an isoprenoid structure that is a feature of many conventional adjuvants, Pristane and Squalene. Chl …

Drug Loading And Release From Polypeptide Multilayer Nanofilms, Yang Zhong

Doctoral Dissertations

Polypeptides, linear macromolecules, are formed from amino acid residues by linkage of peptide bonds. Proteins are polypeptides too, with more complex conformations contributing to specific functionalities. Disulfide bonds are very important to maintain the structure and functions of proteins, which will form between two cysteine (Cys) residues under oxidizing circumstance.

Cys containing polypeptides are designed and synthesized by F-moc (9-Fluorenylmethyloxycarbonyl) chemistry. The number and position of Cys residues can be controlled by amino acid sequences design and following peptide synthesis, which is important to gain insights on the nature of polyelectrolyte multilayer film assembly and stability.

Both commercial and designed …

Design, Synthesis, And Evaluation Of 10-N-Substituted Acridones As Novel Chemosensitizers In Plasmodium Falciparum, Jane X. Kelly, Martin J. Smilkstein, Roland A. Cooper, Kristin D. Lane, Robert A. Johnson, Aaron Janowsky, Rozalia A. Dodean, David J. Hinrichs, Rolf Winter, Michael Riscoe

Biological Sciences Faculty Publications

A series of novel 10-N-substituted acridones, bearing alkyl side chains with tertiary amine groups at the terminal position, were designed, synthesized, and evaluated for the ability to enhance the potency of quinoline drugs against multidrug-resistant (MDR) Plasmodium falciparum malaria parasites. A number of acridone derivatives, with side chains bridged three or more carbon atoms apart between the ring nitrogen and terminal nitrogen, demonstrated chloroquine (CQ)-chemosensitizing activity against the MDR strain of P. falciparum (Dd2). Isobolograrn analysis revealed that selected candidates demonstrated significant synergy with CQ in the CQ-resistant (CQR) parasite Dd2 but only additive (or indifferent) interaction in the CQ-sensitive …

Triterpenoids From The Stem Of Diospyros Glandulosa, Witchuda Thanakijcharoenpath, Orawan Theanphong

The Thai Journal of Pharmaceutical Sciences

Six pentacyclic triterpenoids, including friedelin, β-amyrin, oleanolic acid, lupeol, betulin and ursolic acid, have been isolated from the stem of Diospyros glandulosa Lace. Identification of the isolated compounds was accomplished by analysis of their...

Hplc Determination Of Active Diterpene Lactones From Andrographis Paniculata Nees Planted In Various Seasons And Regions In Thailand, Chamnan Patarapanich, Suwanna Laungcholatan, Nuntakan Mahaverawat, Chaiyo Chaichantipayuth, Sunibhond Pummangura

The Thai Journal of Pharmaceutical Sciences

A quantitative determination of four known diterpene lactone compounds : dehydroandroghapholide (C-2), andrographolide (C-3), neoandrographolide (C-4) and deoxyandrographolide-19-p-D glucoside (C-5) from the leaves of Andrographis paniculata, Nees. has b...

Samambaia - The Future Focus For Indian Researchers In The Treatment Of Psoriasis, Kuntal Das, John Wiiking Einstein

The Thai Journal of Pharmaceutical Sciences

Psoriasis is an issue of global and national public health concern. The traditional use of medicinal plants to treat this disease is widespread throughout India. The present review is an attempt for the beneficial effect of the South American originated ...

Chemometrics-Assisted Spectrophotometric Determination Of Certain Β-Lactam Antibiotics Combinations, Abd El-Maboud I. Mohamed, Hesham Salem, Eman Maher

The Thai Journal of Pharmaceutical Sciences

Multivariate and derivative spectrophotometric techniques (first derivative and derivative ratio) were developed for the determination of four p-lactam antibiotic binary mixtures; ampicillin with flucloxacillin (mix I), ampicillin with dicloxacillin (mix...

Effects Of Drying Temperature And Residual Moisture Content Of Fa-Tha-Li (Andrographis Paniculata (Burm.F.) Nees) Crude Powder For Capsule Preparation, Wipawee Saohin, Preecha Boonchoong, Supachai Lamlikitkuakoon, Itthichai Jamnoiprom, Wittaya Mungdee

The Thai Journal of Pharmaceutical Sciences

The objectives of this research were to study the effects of drying temperature and residual moisture content on total lactones content calculated as andrographolide, flow ability and microbial contamination of Fa-Tha-Li (Andrographis paniculata (Burm.f....

Characterization Of Herpes Simplex Virus Type 1 Selected In Culture For Resistance To Acyclovir Or Foscarnet, Mali Wirotesangthong, Sakulrat Rattanakiat, Katsuhiko Matsui, Vimolmas Lipipun

The Thai Journal of Pharmaceutical Sciences

To determine the characteristics of herpes simplex virus type 1 (HSV-1) resistant to either acyclovir (ACV) or foscarnet (FOS), HSV-1 KOS (wild-type) strain was used for selection of drug-resistant mutants in Vero cells. เท one-step growth curve, all...

An Approach To Developing Dissolution Standards For Turmeric Capsules I: Basket Rotating Method, Nantana Sittichai, Sirichai Krabesri, Ekamol Suthison, Parkpoom Tengamnuay

The Thai Journal of Pharmaceutical Sciences

A simple dissolution test and standard was developed for Turmeric capsules to replace the less discriminating disintegration test stated in the Supplement to Thai Herbal Pharmacopoeia 2004. The proposed test used the basket at 100 rpm and the dissolution...

Comparative Analysis Of Pharmaceutical Care And Traditional Dispensing Role Of Pharmacy, Phantipa Sakthong

The Thai Journal of Pharmaceutical Sciences

Pharmaceutical care is a new professional practice that can give an opportunity to pharmacists to prove themselves as a real health care profession. Pharmaceutical care practitioners are concerned with the outcomes of their services on patients’ life a...

Use Of A Dialyzable Short-Chain Phospholipid For Efficient Preparation Of Virosome Vaccines Against Newcastle Disease, Atthachai Homhuan, Sompol Prakongpan

The Thai Journal of Pharmaceutical Sciences

Virosomes can be regarded as liposomes carrying the spike proteins of enveloped virus on their surface. To further advance the use of virosomes, we developed a solubilization/ reconstitution procedure for construction of Newcastle disease (ND)-virosomes,...

28-Day Repeated Dose Oral Toxicity Study Of Litsea Cubeba Essential Oil In Sprague-Dawley Rats, Siraprapa Tubtim, Ake Wasiksiri

The Thai Journal of Pharmaceutical Sciences

Litsea cubeba essential oil is a raw material source for the isolation of citral that can be converted to a number of important derivatives. This citral-rich essential oil is also increasingly used for flavour and fragrance applications in many household...

Author Index, Keyword Index, N/A

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Microparticulate Drug Reformulation Using Electrostatic Layer -By -Layer Assembly And Co-Entrapment With Anionic Phospholipids In Calcium Carbonate Matrix, Krishna Gopal

Doctoral Dissertations

Non-invasive drug delivery systems for therapeutic drugs are gaining prominence. One such system, the nasal delivery system, shows good prospect for effective delivery of some drugs, however, it is yet to be well established for others. The preparation of a reformulated drug and studying its in vitro characteristics is a crucial step in the process of adapting the new drug for nasal delivery systems. The purpose of this research is to study the effect of electrostatic layer by layer assembly of polyelectrolytes on PROMAXX®insulin microparticles and to study the chemical, physical and corresponding release properties of the resulting formulations. PROMAXX® …

Previous Spatial Memory Training And Nicotine Administration Alleviates Cognitive Deficits Produced By Medial Frontal Cortex Lesions In Rats., Rachel L. Norris

Electronic Theses and Dissertations

Rats were administered nicotine (0.3 mg/kg) for 11 consecutive days before and after an electrolytic medial frontal cortex lesion. Behavioral testing was arranged so that the rats were tested on the RAM 1 day after drug administration followed by behavioral testing on the MWT 19 days after drug treatment, or tested on the MWT 1 day after drug administration followed by testing on the RAM4 days after drug treatment. Results of MWT testing showed that regardless of the drug/behavioral testing interval, lesioned rats given nicotine demonstrated enhancement relative to saline-treated animals. Results of RAM testing showed that nicotine improved performance …

Smoking, Anemia, And Risk Of Oral Clefts In Utah, Melinda Michelle Moss

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cigarette smoke contains sufficient carbon monoxide to induce maternal and fetal hypoxia. Hypoxia is a known teratogen, and consequently maternal smoking has been the focus of many studies on adverse birth outcomes, including cleft lip and palate. Current literature of epidemiological studies on smoking and clefts suggests a modest but statistically significant increase in risk of clefting associated with maternal smoking. A biological condition that may also contribute to hypoxia is anemia. Data from the Utah Child and Family Health Study was used to assess the effects of hypoxia-inducing conditions, maternal smoking, anemia, and their interaction, on the risk of …

Critical Evaluation Of The Claims Made By Pharmaceutical Companies In Drug Promotional Material In Pakistan, Dileep Kumar Rohra, Anwarul Hassan Gilani, Ismail Kamal Memon, Ghazala Perven, Muhammad Talha Khan, Hina Zafar, Rakesh Kumar

Department of Biological & Biomedical Sciences

Background: In Pakistan, there is no mechanism to monitor the drug promotional campaign by pharmaceutical industry despite the fact that there is enough evidence that irrational pharmacotherapy is increasingly encountered even in the developed countries due to unethical practices of pharmaceutical promotion. Objectives. To audit the drug promotional claims made by the pharmaceutical companies in Pakistan.
Methods: Drug promotional pamphlets and brochures containing claims for the drugs, which were circulated by the pharmaceuticalrepresentatives were collected from 122 general practitioners (GPs) from Karachi and Larkana cities of the Sindh Province. The claims were critically analyzed and audited with the help of …

2-Aminoethoxydiphenyl Borate As A Prototype Drug For A Group Of Structurally Related Calcium Channel Blockers In Human Platelets, Yuliya Dobrydneva, Christopher J. Abelt, Beth Dovel, Celina M. Thadigiri, Roy L. Williams, Peter F. Blackmore

Chemistry & Biochemistry Faculty Publications

We have synthesized a series of 2-aminoethoxydiphenyl borate (2-APB, 2,2-diphenyl-1,3,2-oxazaborolidine) analogs and tested their ability to inhibit thrombin-induced Ca²⁺ influx in human platelets. The analogs were either synthesized by adding various substituents to the oxazaborolidine ring (methyl, dimethyl, tert-butyl, phenyl, methyl phenyl, and pyridyl) or increasing the size of the oxazaborolidine ring to seven- and nine-membered rings. NMR analysis of the boron-containing analogs suggests that each of them exist as a ring structure through the formation of an N -> B coordinate bond (except for the hexyl analog). The possibility that these boron-containing compounds formed dimers was also …

Anti-Herpes Simplex Virus Type 2 Activities Of Some Thai Medicinal Plants, Mali Kruawan, Sakulrat Rattanakiat

The Thai Journal of Pharmaceutical Sciences

To explore for plants possessing anti-herpes simplex virus type 2 (anti-HSV-2) activity, 80 extracts prepared from 16 medicinal plants collected in Thailand were investigated in vitro using the plaque reduction assay including the inactivation, pre-treat...

Identification And Antimicrobial Activities Of Actinomycètes From Soils In Samed Island, And Geldanamycin From Strain Pc4-3, Wijittra Kittivachra, Somboon Tanasupawat, Surattana Amnuoypol, Khanit Suwanborirux

The Thai Journal of Pharmaceutical Sciences

In the course of identification and screening for antimicrobial activities of 100 actinomycète isolates from 35 soil and sand samples collected from the shore of Samed Island, Rayong province, 79 isolates were identified as Streptomyces, 20 were Micromo...

Spectrophotometric Determination Of Binary Mixtures Of Prednisolone With Some Antibiotics, Abd El-Maboud I. Mohamed, Hesham Salem, Eman Maher

The Thai Journal of Pharmaceutical Sciences

Multivariate (classical least squares and principal components regression) techniques and derivative spectrophotometric techniques (first derivative and derivative ratio) were developed for the assay of two binary mixtures of prednisolone with tetracycli...