Molecular, Genetic, and Biochemical Nutrition Commons^™

Four Weeks Of High- Versus Low-Load Resistance Training To Failure On The Rate Of Torque Development, Electromechanical Delay, And Contractile Twitch Properties, N.D.M. Jenkins, Terry J. Housh, S.L. Buckner, H.C. Bergstrom, C. M. Smith, Kristen C. Cochrane, E.C. Hill, Amelia A. Miramonti, Richard J. Schmidt, G. O. Johnson, Joel T. Cramer

Department of Nutrition and Health Sciences: Faculty Publications

The purpose of this study was to investigate the effects of 4-weeks of high- versus low-load resistance training to failure on rate of torque development (RTD), electromechanical delay (EMD), and contractile twitch characteristics. Fifteen men (mean±SD; age=21.7±2.4 yrs) were randomly assigned to either a high- (80% 1RM; n=7) or low-load (30% 1RM; n=8) training group and completed elbow flexion resistance training to failure 3 times per week for 4 weeks. The participants were tested at baseline, 2-, and 4-weeks of training. Peak RTD (pRTDV) and RTD at 0-30 (RTD30V), 0-50 (RTD50V), 0-100 (RTD100V), and 0-200 (RTD200V) ms, integrated EMG amplitude …

Soft Drinks Consumption And Child Behaviour Problems: The Role Of Food Insecurity And Sleep Patterns, Christian King

Department of Nutrition and Health Sciences: Faculty Publications

Objective: To examine whether the association between soft drinks consumption and child behaviour problems differs by food security status and sleep patterns in young children.

Design: Cross-sectional observational data from the Fragile Families and Child Wellbeing Study (FFCWS), which collected information on food insecurity, soft drinks consumption, sleep patterns and child behaviour problems. Bivariate and multivariate ordinary least-squares regression analyses predicting child behaviour problems and accounting for socio-economic factors and household characteristics were performed.

Setting: Twenty urban cities in the USA with a population of 200 000 or more.

Subjects: Parental interviews of 2829 children who were about 5 years …

Effect Of Sex On Torque, Recovery, Emg, And Mmg Responses To Fatigue, E.C. Hill, Terry J. Housh, C. M. Smith, Kristen C. Cochrane, N.D.M. Jenkins, Joel T. Cramer, Richard J. Schmidt, G. O. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

The purpose of the present investigation was to examine the effect of sex on maximal voluntary isometric contraction (MVIC) torque and the EMG and MMG responses as a result of fatiguing, intermittent, submaximal (65% of MVIC), isometric elbow flexion muscle contractions. Methods: Eighteen men and women performed MVIC trials before (pretest), after (posttest), and 5-min after (5-min recovery) performing 50 intermittent, submaximal isometric muscle contractions. Surface electromyographic (EMG) and mechanomyographic (MMG) signals were simultaneously recorded from the biceps brachii muscle. Results: As a result of the fatiguing workbout torque decreased similarly from pretest to posttest for both the men (24.0%) …

Effects Of Fatiguing Constant Versus Alternating Intensity Intermittent Isometric Muscle Actions On Maximal Torque And Neuromuscular Responses, C. M. Smith, Terry J. Housh, E.C. Hill, Kristen C. Cochrane, N.D.M. Jenkins, Richard J. Schmidt, G. O. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

Objective: To determine the effects of constant versus alternating applications of torque during fatiguing, intermittent isometric muscle actions of the leg extensors on maximal voluntary isometric contraction (MVIC) torque and neuromuscular responses. Methods: Sixteen subjects performed two protocols, each consisting of 50 intermittent isometric muscle actions of the leg extensors with equal average load at a constant 60% MVIC or alternating 40 then 80% (40/80%) MVIC with a work-to-rest ratio of 6-s on and 2-s off. MVIC torque as well as electromyographic signals from the vastus lateralis (VL), vastus medialis (VM), and rectus femoris (RF) and mechanomyographic signals from the …

Combined Iron Deficiency And Low Aerobic Fitness Doubly Burden Academic Performance Among Women Attending University, Samuel P. Scott, Mary Jane Desouza, Karsten Koehler, Laura E. Murray-Kolb

Department of Nutrition and Health Sciences: Faculty Publications

Background: Academic success is a key determinant of future prospects for students. Cognitive functioning has been related to nutritional and physical factors. Here, we focus on iron status and aerobic fitness in young-adult female students given the high rate of iron deficiency and declines in fitness reported in this population. Objectives: We sought to explore the combined effects of iron status and fitness on academic success and to determine whether these associations are mediated by cognitive performance.

Methods: Women (n = 105) aged 18–35 y were recruited for this cross-sectional study. Data were obtained for iron biomarkers, peak oxygen …

Low Energy Availability In Exercising Men Is Associated With Reduced Leptin And Insulin But Not With Changes In Other Metabolic Hormones, Karsten Koehler, Neele R. Hoerner, Jenna C. Gibbs, Christoph Zinner, Hans Braun, Mary Jane De Souza, Wilhelm Schaenzer

Department of Nutrition and Health Sciences: Faculty Publications

Low energy availability, defined as low caloric intake relative to exercise energy expenditure, has been linked to endocrine alterations frequently observed in chronically energy-deficient exercising women. Our goal was to determine the endocrine effects of low energy availability in exercising men. Six exercising men (V_O2peak : 49.3 ± 2.4 ml · kg⁻¹ · min⁻¹) underwent two conditions of low energy availability (15 kcal · kg⁻¹ fat-free mass [FFM] · day⁻¹) and two energy-balanced conditions (40 kcal · kg⁻¹ FFM · day⁻¹) in randomized order. During one low energy availability and …

A Qualitative Phenomenological Exploration Of Teachers’ Experience With Nutrition Education, Elisha M. Hall, Weiwen Chai, Julie A. Albrecht

Department of Nutrition and Health Sciences: Faculty Publications

Background:

Nutrition education delivered by classroom teachers has become a popular intervention designed to combat childhood obesity. However, few qualitative studies have explored nutrition education with teachers.

Purpose:

The purpose of this study was to explore how elementary teachers describe their experience with nutrition education.

Methods:

A qualitative phenomenological approach was used. Semistructured interviews, observations, and document analysis were conducted with 10 teachers who delivered nutrition education in their classrooms. Inductive coding was used to determine invariant constituents, reduce constituents to categories, and cluster categories into themes. Reliability and validity were accomplished through intercoder agreement, audio recording, triangulation, bracketing, and …

Cranberries And Cancer: An Update Of Preclinical Studies Evaluating The Cancer Inhibitory Potential Of Cranberry And Cranberry Derived Constituents, Katherine M. Weh, Jennifer L. Clarke, Laura A. Kresty

Department of Nutrition and Health Sciences: Faculty Publications

Cranberries are rich in bioactive constituents reported to influence a variety of health benefits, ranging from improved immune function and decreased infections to reduced cardiovascular disease and more recently cancer inhibition. A review of cranberry research targeting cancer revealed positive effects of cranberries or cranberry derived constituents against 17 different cancers utilizing a variety of in vitro techniques, whereas in vivo studies supported the inhibitory action of cranberries toward cancers of the esophagus, stomach, colon, bladder, prostate, glioblastoma and lymphoma. Mechanisms of cranberry-linked cancer inhibition include cellular death induction via apoptosis, necrosis and autophagy; reduction of cellular proliferation; alterations in …

Association Of Individual And Community Factors With C-Reactive Protein And 25-Hydroxyvitamin D: Evidence From The National Health And Nutrition Examination Survey (Nhanes), Weiwen Chai, Jessie X. Fan, Ming Wen

Department of Nutrition and Health Sciences: Faculty Publications

Many individual and community/neighborhood factors may contribute to inflammation and vitamin D deficiency leading to the development of chronic diseases. This study examined the associations of serum Creactive protein (CRP) and 25-hydroxyvitamin D [25(OH)D] levels with individual and community/neighborhood (tract-level or county-level) factors using a nationally representative sample from 2001–2006 National Health and Nutrition Examination Survey (NHANES). Data from the 2001–2006 waves of the continuous NHANES was merged with the 2000 census and other neighborhood data sources constructed using geographic information system. Associations between multilevel factors and biomarker levels were assessed using multilevel random-intercept regression models. 6643 participants aged 19–65 …

Impacts Of High-Protein Oral Nutritional Supplements Among Malnourished Men And Women With Sarcopenia: A Multicenter, Randomized, Double-Blinded, Controlled Trial, Joel T. Cramer, Alfonso J. Cruz-Jentoft, Francesco Landi, Mary Hickson, Mauro Zamboni, Suzette L. Pereira, Deborah S. Hustead, Vikkie A. Mustad

Department of Nutrition and Health Sciences: Faculty Publications

Background: Recent evidence suggests that nutritional interventions may improve muscle outcomes in malnutrition and sarcopenia. Objectives: We evaluated the effects of 2 high-quality oral nutritional supplements (ONS) differing in amount and type of key nutrients in older adult men and women. Design: A multicenter, randomized, double-blinded, controlled clinical trial. Participants: Malnourished and sarcopenic men and women, 65 years and older (n ¼ 330). Intervention: A 24-week intervention period with 2 energy-rich (330 kcal) ONS treatment groups: Control ONS (C_ONS,14 g protein; 147 IU vitamin D₃) versus Experimental ONS (E_ONS, 20 g protein; 499 IU …

Muscle- And Mode-Specific Responses Of The Forearm Flexors To Fatiguing, Concentric Muscle Actions, Ethan C. Hill, Terry J. Housh, Cory M. Smith, Richard Schmidt, Glen O. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

Background: Electromyographic (EMG) and mechanomyographic (MMG) studies of fatigue have generally utilized maximal isometric or dynamic muscle actions, but sport- and work-related activities involve predominately submaximal movements. Therefore, the purpose of the present investigation was to examine the torque, EMG, and MMG responses as a result of submaximal, concentric, isokinetic, forearm flexion muscle actions. Methods: Twelve men performed concentric peak torque (PT) and isometric PT trials before (pretest) and after (posttest) performing 50 submaximal (65% of concentric PT), concentric, isokinetic (60°·s^-1), forearm flexion muscle actions. Surface EMG and MMG signals were simultaneously recorded from the biceps brachii and …

Relationship Between Concentrations Of Lutein And Stard3 Among Pediatric And Geriatric Human Brain Tissue, Jirayu Tanprasertsuk, Binxing Li, Paul S. Bernstein, Rohini Vishwanathan, Mary Ann Johnson, Leonard Poon, Elizabeth J. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

Lutein, a dietary carotenoid, selectively accumulates in human retina and brain. While many epidemiological studies show evidence of a relationship between lutein status and cognitive health, lutein’s selective uptake in human brain tissue and its potential function in early neural development and cognitive health have been poorly evaluated at a molecular level. The objective of this study was to evaluate the cross-sectional relationship between concentrations of brain lutein and StARD3 (identified as its binding protein in retinal tissue) among three age groups: infants (1–4 months, n = 10), older adults (55–86 years, n = 8), and centenarians (98–105 years, n …

Pharmacological And Genetic Modulation Of Rev-Erb Activity And Expression Affects Orexigenic Gene Expression, Ariadna Amador, Yongjun Wang, Subhashis Banerjee, Theodore M. Kameneka, Laura A. Solt, Thomas P. Burris

Department of Nutrition and Health Sciences: Faculty Publications

The nuclear receptors REV-ERBα and REV-ERBβ are transcription factors that play pivotal roles in the regulation of the circadian rhythm and various metabolic processes. The circadian rhythm is an endogenous mechanism, which generates entrainable biological changes that follow a 24-hour period. It regulates a number of physiological processes, including sleep/wakeful cycles and feeding behaviors. We recently demonstrated that REV-ERB-specific small molecules affect sleep and anxiety. The orexinergic system also plays a significant role in mammalian physiology and behavior, including the regulation of sleep and food intake. Importantly, orexin genes are expressed in a circadian manner. Given these overlaps in function …

Food Labeling And Consumer Associations With Health, Safety, And Environment, Joanna K. Sax, Neal Doran

Faculty Scholarship

The food supply is complicated and consumers are increasingly calling for labeling on food to be more informative. In particular, consumers are asking for the labeling of genetically modified organisms (GMO) food based on health, safety and environmental concerns. At issue is whether the labels that are sought would accurately provide the information desired. The present study examined consumer (n = 181) perceptions of health, safety and the environment for foods labeled organic, natural, fat free or low fat, GMO, or non-GMO. Findings indicated that respondents consistently believed that foods labeled GMO are less healthy, safe and environmentally-friendly compared to …

Mechanisms By Which Dietary Ellagic Acid Attenuates Obesity And Obesity-Mediated Metabolic Complications, Inhae Kang

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Ellagic acid (EA) is a polyphenol found in various fruits and plants, such as berries, pomegranates, muscadine grapes, nuts and bark of oak tree. EA has been known to exhibit anti-inflammatory and anti-proliferative effects in various types of cancer. However, little is known about the effects of EA on obesity. Herein, 1) the lipid-lowering role of EA was identified in primary human adipose stem cells (hASCs) and human hepatoma Huh7 cells; 2) the molecular mechanisms by which EA attenuates adipogenesis by epigenetic modification were identified; 3) the effects of EA on high fat and high sucrose-mediated obesity was …

Activation Of Toll-Like Receptor 4 (Tlr4) Attenuates Adaptive Thermogenesis Via Endoplasmic Reticulum Stress, Meshail Okla, Wei Wang, Inhae Kang, Anjeza Pashaj, Timothy P. Carr, Soonkyu Chung

Department of Nutrition and Health Sciences: Faculty Publications

Background: Human obesity is associated with defective brown adipose tissue (BAT) activation.

Results: Toll-like receptor 4(TLR4) activation by high fat diet or lipopolysaccharide impairs adaptive thermogenesis.

Conclusion: Obesity-mediated TLR4 activation represses adaptive thermogenesis through endoplasmic reticulum (ER) stress-mediated mitochondrial dysfunction.

Significance: Inhibition of TLR4/ER stress axis is a novel target to augment BAT activity.

Abstract: Adaptive thermogenesis is the cellular process transforming chemical energy into heat in response to cold. A decrease in adaptive thermogenesis is a contributing factor to obesity. However, the molecular mechanisms responsible for the compromised adaptive thermogenesis in obese subjects have not yet been elucidated. In …

Electromyographic Responses From The Vastus Medialis During Isometric Muscle Actions, C. M. Smith, Terry J. Housh, Trent J. Herda, Jorge M Zuniga, Clayton L. Camic, Haley Bergstrom, D.B. Smith, John R. Weir, Joel T. Cramer, E.C. Hill, Kristen C. Cochrane, N. D. M. Jenkins, R. Schmidt, G. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

This study examined the electromyographic (EMG) responses from the vastus medialis (VM) for electrodes placed over and away from the innervation zone (IZ) during a maximal voluntary isometric contraction (MVIC) and sustained, submaximal isometric muscle action. A linear electrode array was placed on the VM to identify the IZ and muscle fiber pennation angle during an MVIC and sustained isometric muscle action at 50 % MVIC. EMG amplitude and frequency parameters were determined from 7 bipolar channels of the electrode array, including over the IZ, as well as 10 mm, 20 mm and 30 mm proximal and distal to the …

The Effect Of Polyphenol-Rich Black Currant Extract On Lipogenic And Inflammatory Gene Expression In Diet Induced Obesity Mice, Ellen Harness

Honors Scholar Theses

Hyperlipidemia and hyperglycemia frequently occur in obese population. As chronic, low-grade inflammation is closely associated with obesity, we investigated if polyphenol-rich blackcurrant extract (BCE) can prevent inflammation and diet-induced metabolic disturbances in mice. Male C57BL/6J mice were given a modified AIN-93M control diet containing high fat/high cholesterol (16% fat, 0.25% cholesterol by weight) or the same diet supplemented with 0.1% BCE (wt/wt) for 12 weeks. No significant differences in total body weight or liver weight occurred between the two groups. BCE-fed mice had fewer crown-like structures (CLS) with concomitant decreases in mRNA abundance of F4/80, CD68, and inhibitor of nuclear …

Attenuation Of Mtorc1-Driven Secretion Of Lipoproteins And Triacylglycerides By Short Chain Fatty Acids: Mechanistic Insight Into The Pathogenesis Of Hypertriglyceridemia, Joseph L. Roberts

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The mechanistic target of rapamycin complex 1 (mTORC1) is a serine/threonine kinase that drives several anabolic processes including lipid synthesis, protein synthesis, and adipogenesis. mTORC1 is highly active in the livers of obese rodents, in overnutrition, and is implicated in the development of obesity related metabolic disorders, including the overproduction of atherogenic lipoproteins. Direct inhibition of mTORC1 is not a viable treatment strategy because it prevents feedback inhibition of the insulin-signaling cascade, leading to increased lipid synthesis and secretion of lipoproteins. Thus, therapeutic approaches that drive catabolic pathways are considered promising mechanisms for overcoming mTORC1-driven anabolism. Human liver HepG2 cells …

Food Resource Management Education With Snap Participation Improves Food Security, Lucia Kaiser, Virginia Chaidez, Susan Algert, Marcel Horowitz, Anna Martin, Concepcion Mendoza, Marisa Neelon, David C. Ginsburg

Department of Nutrition and Health Sciences: Faculty Publications

Objective: To determine the influence of Supplemental Nutrition Assistance Program (SNAP) and participant demographics on nutrition education outcomes.

Methods: At program enrollment (pre) and 1 month later (post), a statewide convenience sample of adults, who participated in the Plan, Shop, Save, and Cook program, completed a 7-item questionnaire to evaluate change in resource management skills (RMS) and running out of food before the end of the month.

Results: Percent of participants (n = 3,744) who reported behavioral improvements in RMS ranged from 38.8%in comparing prices to 54% in reading labels. Female gender and Hispanic ethnicity were positively related to pre–post …

Micrornas Are Absorbed In Biologically Meaningful Amounts From Nutritionally Relevant Doses Of Cow’S Milk And Chicken Eggs And Affect Gene Expression In Peripheral Blood Mononuclear Cells, Cell Cultures, And Mouse Livers, Scott Baier

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Throughout the twenty-first century, evidence has been continually increasing to show the importance of epigenetic regulation in health. While the term “epigenetics” can be applied to many different processes, the focus of this dissertation will be on microRNAs and chromatin structure. Ultimately, both of these forms of epigenetic regulation can be used to fine tune gene expression based on environmental cues. The first three chapters of the dissertation focus on microRNA bioavailability, stability, and function from two commonly consumed food products: cow’s milk and chicken eggs. This important work has been the first of its kind to demonstrate the bioavailability …

Stability And Biological Activity Of Dietary Micrornas, Katherine Howard

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

MicroRNAs play important roles in gene regulation by binding to complimentary sites at the 3’ untranslated region of target mRNA molecules. Binding results in inhibition or degradation of target mRNA. Many bovine and chicken microRNA are homologous with human counterparts enabling gene regulation. A recent study in our lab provided undisputable evidence that endogenous milk microRNAs are bioavailable in humans; resulting in regulation of human gene expression. Based on these findings, we wanted to explore the possibility that other exogenous food borne microRNAs are able to be absorbed through the diet. My thesis surrounds two aims: 1) assessing the stability …

Bacterial Expression Of Plant Pyruvate Kinase, Kristina Fuller

STAR Program Research Presentations

Pyruvate kinases serve an important role in regulating glycolysis pathways. Different pyruvate kinase isozymes can dictate which of several different products will be formed during the final step of glycolysis. Being able to reliably copy active pyruvate kinases (PK) would allow study of the different isozymes, with the goal of eventually creating plants that are more nutritionally beneficial for humans. This study focused on improving the protocol for replicating each of two isolated PK isozymes from the model plant Arabidopsis thaliana. Previous attempts had resulted in the PK denaturing and consequently precipitating out of solution and so we manipulated the …

Food Safety Knowledge, Attitudes, And Behaviors Of Native American Families With Young Children: A Mixed Methods Study, Kara Vlasin-Marty, Paula Ritter-Gooder, Julie A. Albrecht

Department of Nutrition and Health Sciences: Faculty Publications

Children are at increased risk for foodborne illness due to underdeveloped immune system. Limited research has been reported on food safety knowledge of Native American families with children 10 years of age and younger. This study was conducted to determine the food safety knowledge, attitudes, and behaviors of the main food preparer in these families by collecting quantitative and qualitative data simultaneously in a mixed method approach. A food safety knowledge survey created using FightBAC!™ concepts was administered prior to focus groups discussions held in Native American communities using a script based upon the Health Belief Model. Quantitative data were …

Development And Validation Of A Social Cognitive Theory-Based Survey For Elementary Nutrition Education Program, Elisha Hall, Weiwen Chai, Wanda Koszewski, Julie A. Albrecht

Department of Nutrition and Health Sciences: Faculty Publications

Background: The Social Cognitive Theory (SCT) is a widely used model for developing elementary nutrition education programs; however, few instruments are available to assess the impact of such programs on the main constructs of the SCT. The purposes of this study were: 1) to develop and validate a SCT-based survey instrument that focuses on knowledge, behavior, and self-efficacy for fifth grade students; 2) to assess the relationships between knowledge, behavior, and self-efficacy; and 3) to assess knowledge, behavior, and self-efficacy for healthy eating among the fifth grade students.

Methods: A 40-item instrument was developed and validated using content validity …

Nutrition-Related Policy And Environmental Strategies To Prevent Obesity In Rural Communities: A Systematic Review Of The Literature, 2002–2013, Larissa Calancie, Jennifer Leeman, Stephanie B. Jilcott Pitts, Laura Kettel Khan, Kelly R. Evenson, Michelle Schreiner, Carmen Byker, Clint Owens, Jared Mcguirt, Ellen Barnidge, Wesley Dean, Donna Johnson, Jane Kolodinsky, Emily Piltch, Courtney Pinard, Emilee Quinn, Lauren Whetstone, Alice Ammerman, Sheila Fleischhacker

Department of Nutrition and Health Sciences: Faculty Publications

Residents of rural communities in the United States are at higher risk for obesity than their urban and suburban counterparts. Policy and environmental-change strategies supporting healthier dietary intake can prevent obesity and promote health equity. Evidence in support of these strategies is based largely on urban and suburban studies; little is known about use of these strategies in rural communities. The purpose of this review was to synthesize available evidence on the adaptation, implementation, and effectiveness of policy and environmental obesity-prevention strategies in rural settings.

Reductions In Urinary Collection Frequency For Assessment Of Reproductive Hormones Provide Physiologically Representative Exposure And Mean Concentrations When Compared With Daily Collection, Heather C.M. Allaway, Nancy J. Williams, Rebecca J. Mallinson, Karsten Koehler, Mary Jane De Souza

Department of Nutrition and Health Sciences: Faculty Publications

Objective — To determine if reducing the frequency of urinary sample collection from daily to 5, 3, or 2 days per week during a menstrual cycle or 28-day amenorrheic monitoring period provide accurate representations of the reproductive hormone metabolites estrone- 1-glucuronide (E1G) and pregnanediol glucuronide (PdG) exposure and mean concentrations.

Methods — Exercising women presenting with eumenorrhea or exercise-associated menstrual disturbances collected daily urine samples for the assessment of E1G and PdG concentrations. After enzyme immunoassay analysis of the daily samples, E1G and PdG data were systematically removed from each menstrual cycle or amenorrheic monitoring period to mimic three reduced …

Physiological Responses Underlying The Perception Of Effort During Moderate And Heavy Intensity Cycle Ergometry, Kristen C. Cochrane, Terry J. Housh, Ethan C. Hill, Cory M. Smith, Nathaniel D.M. Jenkins, Joel T. Cramer, Glen O. Johnson, Richard J. Schmidt

Department of Nutrition and Health Sciences: Faculty Publications

This study examined patterns of responses for physiological and perceptual variables during cycle ergometry at a constant rate of perceived exertion (RPE) within the moderate and heavy exercise intensity domains. Nineteen (mean age 21.3 ± 0.5 years; 43.4 ± 2.0 mL·kg⁻¹·min⁻¹ VO_2Peak) moderately trained cyclists performed an incremental test to exhaustion and two 60 min constant RPE rides at the RPE corresponding to the gas exchange threshold (RPE_GET) and 15% above the GET (RPE_GET+15%). Oxygen consumption (VO₂), respiratory exchange ratio (RER), heart rate (HR), minute ventilation (V_E), …

Cloning And Expression Of Ebi3 And P28 Subunits Of Human Interleukin 27 In E. Coli, Ameneh Koochaki, Mojgan Bandehpour, Sam Massahi, Mahrou Sadri, Bahram Kazemi

Department of Nutrition and Health Sciences: Faculty Publications

Background: Interleukin (IL)-27 is a heterodimeric cytokine belonging to IL-12 and IL-23 families, secreted by antigen presenting cells(APCs). The IL-27 is composed of 2 subunits: Epstein-Barr virus induced gene 3 (EBI3) and p28. IL-27 is an anti-inflammatory cytokine which has an inhibitory effect on Th17 population and suppress the IL-17 expression. It is suggested that IL-27 could be a potent drug candidate for treating auto immune diseases.

Materials and Methods: The EBI3 and p28 subunits of human interleukin 27 were constructed into plasmid vectors; they sub-cloned into pETDuet-1 expression vector in restriction sites of BamHI, SacI and NotI. Subsequently the …

Effects Of Partners Together In Health (Path) Intervention On Physical Activity And Healthy Eating Behaviors: A Pilot Study, Bernice C. Yates, Joseph Norman, Jane Meza, Kaye L. Stanek Krogstrand, Susana Harrington, Scott Shurmur, Matthew Johnson, Karen Schumacher

Department of Nutrition and Health Sciences: Faculty Publications

Background—Despite proven efficacy of cardiac rehabilitation (CR) in helping patients initiate physical activity and healthy eating changes, less than 50% of CR participants maintain changes 6 months later.

Objective—The objective of this feasibility study was to test the Partners Together in Health (PaTH) Intervention versus usual care (UC) in improving physical activity and healthy eating behaviors in coronary artery bypass graft (CABG) surgery patients and spouses.

Methods—An experimental, two-group (n = 17 couples/group), repeated measures design was used. CABG patients in both groups participated in Phase II outpatient CR. Spouses in the PaTH group attended CR with the patient and …