Molecular, Genetic, and Biochemical Nutrition Commons^™

Mechanomyographic Amplitude And Frequency Responses During Dynamic Muscle Actions: A Comprehensive Review, Travis W. Beck, Terry J. Housh, Joel T. Cramer, Joseph P. Weir, Glen O. Johnson, Jared W. Coburn, Moh H. Malek, Michelle Mielke

Department of Nutrition and Health Sciences: Faculty Publications

The purpose of this review is to examine the literature that has investigated mechanomyographic (MMG) amplitude and frequency responses during dynamic muscle actions. To date, the majority of MMG research has focused on isometric muscle actions. Recent studies, however, have examined the MMG time and/or frequency domain responses during various types of dynamic activities, including dynamic constant external resistance (DCER) and isokinetic muscle actions, as well as cycle ergometry. Despite the potential influences of factors such as changes in muscle length and the thickness of the tissue between the muscle and the MMG sensor, there is convincing evidence that during …

Orientation To Nutrition Care Process Standards Improves Nutrition Care Documentation By Nutrition Practitioners, Nancy Hakel-Smith, Nancy M. Lewis, Kent M. Eskridge

Department of Nutrition and Health Sciences: Faculty Publications

Objective — To compare documentation of two groups of clinical nutrition practitioners for evidence of the nutrition care process.

Design — This study used a comparative descriptive design. A retrospective chart review was conducted on all nutrition documentation in closed patient records. Documentation of two groups of nutrition practitioners (institution A = practitioners who received initial orientation and routine reinforcement in use of nutrition care process standards; institution B = practitioners who received orientation in use of a further assessment and medical nutrition therapy intervention procedure) was compared for evidence of a six-step nutrition care process.

Sample/Setting — The sample …

Serum Lipids Of Physically Active Adults Consuming Omega-3 Fatty Acid–Enriched Eggs Or Conventional Eggs, Carrie A. Sindelar, Sarah B. Scheerger, Sheri L. Plugge, Kent M. Eskridge, Rosemary C. Wander, Nancy M. Lewis

Department of Nutrition and Health Sciences: Faculty Publications

This study was designed to compare the effects of the consumption of one omega-3 (n-3) polyunsaturated fatty acid (PUFA)–enriched egg or one conventional egg on serum lipids in physically active adults. A total of 12 adults (mean age 33 ± 7 years, mean body mass index [BMI] 24 ± 3) were recruited, and dietary treatments were randomly assigned. After a 2-week lead-in period (baseline), participants received each 4-week treatment in a crossover arrangement with a 4-week washout period between treatments. Participants completed a 3-day food record at baseline and during each treatment period. Food records were analyzed for carbohydrates, protein, …

Investigation Of The Effect Of Sulfitolysis On The Functional Properties And Extrusion Performance Of Whey Protein Concentrate, David P. Taylor

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Whey proteins have restricted use in many food applications because of limited functional properties. Whey proteins' relatively high content of disulfide bonds may be responsible for their lack of functionality, especially in extrusion applications.

To determine the effect of disulfide bond content on functional properties and extrudate performance, whey protein concentrate was treated with sodium sulfite to achieve four levels of disulfide bond sulfonation (0, 31, 54, and 71%). Sulfonated whey protein functional properties, extrusion-expanded snack properties (32% total protein), and extrusion-textured fibrous product properties (48% protein) were determined. Correlation analysis was performed to determine relationships between functional properties and …

Biotechnology And The Law: A Consideration Of Intellectual Property Rights And Related Social Issues, Michael D. Mehta

The University of New Hampshire Law Review

[Excerpt] “Recent advances in biotechnology are expected by many to improve crop yield, reduce reliance on agricultural inputs like pesticides and herbicides, alleviate world hunger, improve the safety and effectiveness of pharmaceuticals, assist in the discovery of genes that trigger diseases like cancer, and make more efficient our legal institutions through DNA testing. Clearly, innovations in biotechnology are a powerful force for social change, and they pose unique challenges and opportunities for legal scholars and institutions. This section of the Pierce Law Review focuses on the interface between law and technology by examining how innovations in biotechnology accelerate debates about …

Physiological Importance Of 1,25(Oh)2D3 Membrane-Initiated And Nuclear Actions In Chickens As A Function Of Growth, And Maturation In Male And Female Chickens, Birgitta Larsson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

It is well established that l,25(OH)₂D₃ is a major regulator of calcium homeostasis. The steroid exerts its effects on its target organs by two mechanisms, a slow mechanism mediated by nuclear vitamin D receptors (nVDR), and a rapid mechanism mediated by the l,25(OH)₂D₃ membrane-associated rapid response steroid binding protein (l,25D₃-MARRS bp). In this dissertation, the physiological relevance of membrane initiated steroid signaling was investigated by studying the correlation of age in male and female chickens with the magnitude of responses to l,25(OH)₂D₃ in duodena from 7-, 14-, 28-, and …

Biomarkers Of Bone Turnover In Vegetarians And Omnivores, Susan Kim Louie Lue

Loma Linda University Electronic Theses, Dissertations & Projects

A cross-sectional study on fasting serum and 24-hour urine samples were used to determine whether biochemical markers of bone turnover, resorption and formation, differed in a group of 50 healthy premenopausal Caucasian women eating vegan, lactoovovegetarian(LOV) and omnivorous diets.

The women, ages 20-42, had participated in a study of bone mineral density(BMD). The three groups were comparable in mean age, BMI, calorie intake, calories spent in exercise, kcal/kg body weight, protein/day, protein/kg body weight and urinary creatinine.

Biomarkers included those of bone resorption—pyridinoline, deoxypyridinoline, and N-telopeptides, and of bone turnover—serum and urinary osteocalcin(OC), skeletal alkaline phosphatase, and propeptide-type-I-carboxyterminal-procollagen(PICP). In all …

Public Perceptions Of Genetically Engineered Foods: Playing God Or Trusting Science, Michael D. Mehta

RISK: Health, Safety & Environment (1990-2002)

The author considers whether levels of religiosity or scientism affect public perceptions of genetically engineered foods.

Studies On The Formation Of Dna-Cationic Lipid Composite Films And Dna Hybridization In The Composites, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Krishna N. Ganesh

Faculty Works

The formation of composite films of double-stranded DNA and cationic lipid molecules (octadecylamine, ODA) and the hybridization of complementary single-stranded DNA molecules in such composite films are demonstrated. The immobilization of DNA is accomplished by simple immersion of a thermally evaporated ODA film in the DNA solution at close to physiological pH. The entrapment of the DNA molecules in the cationic lipid film is dominated by attractive electrostatic interaction between the negatively charged phosphate backbone of the DNA molecules and the protonated amine molecules in the thermally evaporated film and has been quantified using quartz crystal microgravimetry (QCM). Fluorescence studies …

Relationship Between Cag Repeats Of The N Terminal Region Of The Androgen Receptor And Body Shape, Michael John Wen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Androgen receptor (AR) gene CAG polymorphisms may be associated with body shape, and are associated with certain breast and prostate cancers. In addition, body shape is associated with risk for a variety of diseases, including heart disease, diabetes, and certain forms of cancer. The CAG repeat in exon l of the AR gene was quantified using Perkin Elmer Applied Biosystems GeneScan analysis software in 96 and 59 healthy Caucasian men and women, respectively, who were over the age of 50 years. All participants had body measurements taken and donated a blood sample. Waist measurements included circumferences at the 1) umbilicus …

Cationic Surfactant Mediated Hybridization And Hydrophobization Of Dna Molecules At The Liquid/Liquid Interface And Their Phase Transfer, Murali Sastry, Ashavani Kumar, Mrunalini Pattarkine, Vidya Ramakrishnan, Krishna N. Ganesh

Faculty Works

Hybridization of complementary oligonucleotides mediated by a cationic surfactant at the water/hexane interface leads to hydrophobic, double-helical DNA which may be readily phase transferred to the organic phase and cast into thin films on solid substrates.

Hybridization Of Dna By Sequential Immobilization Of Oligonucleotides At The Air-Water Interface, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh

Faculty Works

The hybridization of DNA by sequential electrostatic and hydrogen-bonding immobilization of single-stranded complementary oligonucleotides at the air-water interface with cationic Langmuir monolayers is demonstrated. The complexation of the single-stranded DNA molecules with octadecylamine (ODA) Langmuir monolayers was followed in time by monitoring the pressure-area isotherms. A large (and slow) expansion of the ODA monolayer was observed during each stage of complexation in the following sequence: primary single-stranded DNA followed by complementary single-stranded DNA followed by the intercalator, ethidium bromide. Langmuir-Blodgett (LB) films of the ODA-DNA complex were formed on different substrates and characterized using quartz-crystal microgravimetry (QCM), Fourier transform infrared …

The Effects Of Free Fatty Acids On Gluconeogenesis And Glycogenolysis In Normal Subjects, X Chen, N Iqbal, G Boden

Rowan-Virtua School of Osteopathic Medicine Departmental Research

We have quantitatively determined gluconeogenesis (GNG) from all precursors, using a novel method employing 2H20 to address the question of whether changes in plasma free fatty acids (FFA) affect GNG in healthy, nonobese subjects. In the first study (n = 6), plasma FFA were lowered at 16 to 20 hours with nicotinic acid (NA) and were then allowed to rise at 20 to 24 hours (FFA rebound after administration of NA). FFA decreased from 387 microM at 16 hours to 43 microM at 20 hours, and then rebounded to 1,823 microM at 24 hours. GNG decreased from 58.1% at 16 …

Identification Of A Family Of Zinc Transporter Genes From Arabidopsis That Respond To Zinc Deficiency, Natasha Grotz, Tama Fox, Erin Connolly, Walter Park, Mary Lou Guerinot, David Eide

Dartmouth Scholarship

Millions of people worldwide suffer from nutritional imbalances of essential metals like zinc. These same metals, along with pollutants like cadmium and lead, contaminate soils at many sites around the world. In addition to posing a threat to human health, these metals can poison plants, livestock, and wildlife. Deciphering how metals are absorbed, transported, and incorporated as protein cofactors may help solve both of these problems. For example, edible plants could be engineered to serve as better dietary sources of metal nutrients, and other plant species could be tailored to remove metal ions from contaminated soils. We report here the …

Anion Induced Blue To Purple Transition In Bacteriorhodopsin, Mrunalini Pattarkine, Anil K. Singh

Faculty Works

Purple membrane (PM, λ" role="presentation">λmax" role="presentation">max 570 nm) of H. halobium on treatment with sulphuric acid changes its colour to blue (λ" role="presentation">λmax" role="presentation">max 608 nm). The purple chromophore can be regenerated from the blue chromophore by exogeneous addition of anions such as CI−" role="presentation">− and HPO42−" role="presentation">2−4. Chloride ion is found to be more effective than the dibasic phosphate ion in regenerating the purple chromophore. Nevertheless, one thing common to the anion regeneration is that both CI−" role="presentation">− and HPO42−" role="presentation">2−4 show marked pH effect. At pH 1.0 the efficiency of …

The Effect Of A 20-25 Calories % Fat Diet On Plasma Thromboxane B₂ Levels In Human Subjects, Carolyn Wernick

Loma Linda University Electronic Theses, Dissertations & Projects

Fourteen males with elevated blood lipids were given a 20-2.5 Calories % fat diet for 28 days. Fasting blood samples were drawn at zero, two, and four weeks and analyzed for plasma TXB₂ and serum lipids. Plasma TXB₂ was significantly increased the first two weeks with a subsequent decrease the final two weeks. Values were still significantly elevated at four weeks when compared with initial values. Serum cholesterol, HDL cholesterol, and LDL cholesterol were significantly decreased over the four-week period. No significant changes were observed in serum triglycerides.

Variations In Volatiles From Lamb Adipose Tissue, Yn-Mei Chen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Lamb adipose tissue, representing 4 breeds, 3 sex-types and 3 weight categories, were studied to measure the variation in the flavor profile between individual animals, based on breed, sex- type and carcass weight. The flavor concentrates were prepared by steam distillation, ether extraction and fractionation. Pooled samples were prepared in the same way to measure any variations due to methodology.

Gas chromatographic separation and statistical analysis, based on analysis of variance, indicated that 21 peaks out of 124 peaks showed significant differences (at 5% level or 1% level). Among these 21 peaks, 5 were affected by breed, 4 by sex-type, …

Rate Of Depletion Of Liver Vitamin A In Chicks, Weanling Rabbits And Weanling Rats Fed Vitamin A-Free Diets, J.J. Tlustohowicz, R.C. Hall, J.E. Rousseau, H.I. Frier, S. Donoghue

Storrs Agricultural Experiment Station

No abstract provided.

Magnesium Deficiency And Excitability In The Rat: An Examination Of Selected Biochemical And Physiological Events Relating Magnesium Status To Behavior, Douglas Robinson Buck

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effect of Mg status on the behavior of rats, as determined by nonspecific excitability level and audiogenic seizure susceptibility, was investigated. Also, selected biochemical and neurological mechanisms mediating the chain of events from dietary magnesium deficit to the hyperexcitability symptoms were examined. Weanling rats fed a low magnesium (10 ppm) diet for 14 days had reduced serum, cerebrospinal fluid and brain magnesium concentrations, and increased brain (Na⁺ K⁺) -ATPase activity. They exhibited increased NEL and became highly susceptible to audiogenic seizures. Through dietary manipulation and intraperitoneal and cerebral intraventricular injections, it was possible selectively to alter …

Biochemical Investigations Of Black Gram (Phaseolus Mungo L.) And Rice (Oryza Sativa L.) Proteins And Their Improved Nutritional Functionality In The Fermented Product—Idli, Vinodkumar W. Padhye

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The objectives of this investigation have been to characterize black gram (Phaseolus mungo L.) and rice (Oryza sativa L.) proteins and to study changes in their nutritional value due to fermentation. Black gram, the legume chosen for this work, is one of the most important legume crops throughout a large part of the tropics.

The protein content of 60 mesh, dehydrated, defatted black gram meal was 28.5 percent. Sodium carbonate (0.5-1.0 percent), tetra-sodium pyrophosphate (0.5 percent), and sodium dodecyl sulfate (SDS) (0.5-5 percent) proved to be the potential protein solubilizers as they extracted more than 76 grams of …

Effect Of Zinc Nutriture And Contraceptive Steroid Injection On Body Composition Of Young Female Rats, Sang Ai Hahn

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Forty-nine female rats initially weighing about 95 grams were used in this experiment to determine the effect of contraceptive hormones on body composition in zinc deficient and zinc supplemented rats. Four treatment groups were: 2 ppm of dietary zinc with daily contraceptive steroid injections, or with placebo injection, and 140 ppm of dietary zinc with contraceptive steroid injection or with placebo. The daily injected steroids consisted of 0.1ug of Ethynyl Estradiol and 0.1mg of Medroxyprogesterone in 0.1ml of corn oil. Non-hormone treated groups received 0.1ml of plain corn oil. All injections were administered into the Gluteus Maximus muscle. Food intake, …

The Effects Of Sources And Levels Of Vitamin A And D On Some Aspects Of Sulfur Metabolism, Akbar Malekpour

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Two trials were conducted using 48 infant pigs of both sexes. Pigs were taken from the sow when three to seven days old and placed in wire-bottomed metal cages by pairs. Animals in the first trial received synthetic diets containing 100 or 1000 international units of ergocalciferol and 1000 or 4000 international units of retinyl acetate or four or sixteen milligrams of beta carotene per kilogram of feed. Baby pigs in the second trial received the same synthetic diet plus 1000 international units of ergocalciferol (D₂) or cholecalciferol (D₃) and 2000 international units of vitamin A …

The Pig's Need For Minerals, N W. Godfrey

Journal of the Department of Agriculture, Western Australia, Series 4

Only 16 of the one hundred odd chemical elements known are considered to be essential mineral elements for pigs.

As some of these are required in much smaller quantities than others, they are referred to as trace or minor elements, while the remainder are called major elements.

5 Β- Pregnane- 3Α- 20Α- Diol Excretion In Urine Of University Women During The Menstrual Period, Rita Shin-Hui Liao

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Five normal healthy young university women while on self-chosen diets and living under normal conditions served as experimental subjects in this study. Urinary pregnanediol was measured for 10 days of the menstrual cycle. Meal frequency (3 meals vs. 2 meals with no breakfast) was also included in the study.

A modification of the method of Eberlein and Bongiovanni on thin layer chromatography was used to determine pregnanediol in the urine sample.

Urine pregnanediol excretion was in the pattern as investigated by other workers. The values rose in the latter half of the menstrual cycle, and fell prior to the onset …

Vegetable Entrees In Nitrogen Balance And Obesity Studies, Gloria Tyndall

Loma Linda University Electronic Theses, Dissertations & Projects

The first study was designed to evaluate by the nitrogen balance method the protein quality of a pure-vegetarian diet containing about 6 grams of nitrogen per day. The group as a whole maintained nitrogen equilibrium the average being - 0.11 grams of nitrogen per day. Two subjects were not in nitrogen balance, however, factors other than diet may have contributed to these results and are discussed in the thesis. Hemoglobin, serum cholesterol and total serum proteins did not change significantly during the study. The average true digestibility of 5 of the subjects was 85 percent. It was concluded that this …

A Review Of Drug Addiction And A Study Of Its Relation To Carbohydrate Metabolism, Eppie Chung Hartsuiker

Loma Linda University Electronic Theses, Dissertations & Projects

The specter of addiction eventually involves everyone of the community. It is a three-fold problem of socio-economic, psychological, and physiological factors. The least understood is the physiological or biochemical aspect of addiction.

It has been postulated that an upset in carbohydrate metabolism may be a biochemical cause of addiction. Several avenues were employed to investigate this possible cause of addiction.

1. A diet survey was conducted and revealed a greater consumption of refined carbohydrates, confections and alcoholic beverages in addicts.

2. Five-hour oral glucose tolerance tests were performed on 7 addicts and 12 non-addicts. These tests revealed that there were decreased glucose …

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The Influence Of Nitrogen And Phosphorus Supplements On The Composition Of The Ruminal Ingesta Of Sheep Grazing Desert Range Forage, James G. Morris

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Approximately 46 million acres or 88 per cent of the land area of the state of Utah has been classified by Reuss and Blanch (1951) as "range land open for grazing." Although some of this area cannot be grazed because of inaccessibility, lack of water, poison plants, and similar factors, it is estimated that some 40 million acres or 78 per cent of the total land area is available for grazing. Most of this grazing land is federally owned (73 per cent), while only 22 per cent is privately held, and 5 per cent is state owned.

The Effect Of Adrenaline Injection On Glycogenolysis And Glycogenesis In Rat Livers, Virginia Mary Zingsheim

Bachelors’ Theses

Present day knowledge concerning the liver is derived from many sources and the accumulation of many methods of investigation. Sugar, which is needed by the body for energy, is stored in the liver as glycogen. Claude Bernard (26), an eminent physiologist, was the first to suspect that carbohydrates were stored in the liver. Faulty glycogen synthesis and hydrolysis results in one of our most widespread diseases, diabetes. Thus, many biochemists and physiologists have tried to find the mechanism of the process, and the primary and secondary facts which affect glycogenesis and glycogenolysis. Although the chemical secretions and hormones of such …

Studies On Nutritional Muscular Dystrophy, I. Dietary Factors, Ii. Fibrosis And Lipomatosis Of Tissues, Violet Myrtle Wilder

Department of Biochemistry: Dissertations, Theses, and Student Research

dissertation published as three peer-reviewed papers:

Sergius Morgulis, Violet M. Wilder, and S. H. Eppstein. (September 1938). Further studies on dietary factors associated with nutritional muscular dystrophy. Journal of Nutrition 16(3): 219-227.

Howard C. Spencer, Sergius Morgulis, and Violet M. Wilder. (August 1937). A micromethod for the determination of gelatin and a study of the collagen content of muscles from normal and dystrophic rabbits. Journal of Biological Chemistry 120(1): 257-266.

Sergius Morgulis, Violet M. Wilder, Howard C. Spencer, and S. H. Eppstein. (August 1938). Studies on the lipid content of normal and dystrophic rabbits. Journal of Biological Chemistry 124(3): 755-766.