Exploring The Genetic Diversity And Population Structure Of Serbian And Selected European Bread Wheat Cultivars Through Ipbs-Retrotransposon Markers, Rada Šućur, Amjad Ali, Parnaz Mortazavi, Muhammad Tanveer Altaf, Muhammed Tatar, Muhammad Azhar Nadeem, Bojan Jocković, Velimir Mladenov, Sotirios Fragkostefanakis, Yong Suk Chung, Faheem Shehzad Baloch

Turkish Journal of Agriculture and Forestry

Bread wheat is a globally vital crop, sustaining millions and contributing to food security. The present study investigated the molecular characterization of 60 bread wheat accessions, using 12 interprimer binding site (iPBS) retrotransposon markers, which yielded a total of 260 distinct bands. Out of the 260 bands, 42 were monomorphic while the remaining 218 (83.84%) were polymorphic, with polymorphism information content values ranging from 0.38 to 0.45. Genetic diversity indices, including Shannon’s information index (I = 0.01–0.53), effective number of alleles (Ne = 1.00–1.63), gene diversity (He = 0.0037–0.36), and marker index (MI = 0.31– 0.51), revealed moderate variability across …

Effects Of Storage Temperatures On Quality And Phytochemical Properties Of The Hawthorn (Crataegus Azarolus L.) During Storage And Shelf Life, Ahmet Erhan Özdemi̇r, Oğuzhan Çalişkan, Safder Bayazit, Kazi̇m Gündüz, Nurdan Tuna Güneş, Zafer Karaşahi̇n, Derya Kiliç, Ramazan Bestami̇ Karahan

Turkish Journal of Agriculture and Forestry

This study aimed to investigate the effects of storage temperature on the quality and phytochemical characteristics in Sultan hawthorn cultivar during cold storage and shelf life. Fruits were stored at 0 ± 0.5 °C and 4 ± 0.5 °C and 90 ± 5% relative humidity (RH) for 6 months in cold storage and were analyzed every month. In addition, hawthorn fruits were kept at 20 ± 0.5 °C and 75 ± 5% RH for 7 days, and their shelf life was investigated at monthly intervals. Weight loss, appearance, the incidence of physiological disorders and fungal decay, fruit skin color, firmness, …

Monitoring The Developmental Stages Of Calendula Officinalis L. Nutrients Efficacy Under Hot Weather Mitigation Through Nutrients In Haripur, Pakistan, Waseem Ahmed, Rafia Azmat, Shah Masaud Khan, Abdul Qayyum, Waseem Hassan, Adnan Noor Shah, Muhammad Hadi Abbas, Fatima M. Abbas, Tahani A.Y. Asseri, Ahmet Hakan Ürüşan

Turkish Journal of Agriculture and Forestry

This study examines the effect of different doses of N and P fertilizer on Calendula officinalis L. (marigold) during the hot weather in summer (May–July) in Pakistan. The aim was to determine whether fertilization can help minimize the impacts of climate change. During the summer peak seasons (May, June, and July), the trial was conducted in potted plants with different N and P application levels to monitor the quality and quantity of C. officinalis. The results showed that the interactive effects (N × P) gave higher values for plant height, number of leaves, fresh weight, leaf length, and number of …

Chemical Weed Control Strategies For Sweet Corn In Türkiye’S Diverse Production Areas, Zübeyde Fi̇li̇z Arslan, Ahmet Uludağ, Hüseyi̇n Güngör, Ti̇muçi̇n Taş, Emi̇ne Aydin, Martin M. Williams Ii

Turkish Journal of Agriculture and Forestry

Sweet corn production in Türkiye is failing to match growing demand from consumers and the food industry. Weeds severely limit sweet corn production, yet only a single herbicide is registered in Türkiye. Due to labor costs, hand weeding is no longer affordable and growers are demanding cost-effective weed control strategies for sweet corn production. Nine treatments including preemergence (PRE) and/or postemergence (POST) herbicides were tested across the country’s diverse growing environments. Compared to the weedy control, all treatments increased the number of marketable ears. Only two treatments—s-metolachlor plus terbuthylazine followed by isoxaflutole plus cyprosulfamide plus theincarbazone-methyl and dimethenamid followed by …

Green Synthesis Of Silver Nanoparticles From Melanoleuca Grammopodia: Changes In Bioactive Components, Antidiabetic Properties And Bioaccessibility, İsmai̇l Acar, Emi̇ne Okumuş, Arzu Karataş

Turkish Journal of Agriculture and Forestry

Green synthesis of silver nanoparticles (AgNPs) using plant materials allows the production of low-cost and biocompatible nanoparticles without the use of toxic compounds or solvents. In this study, biogenic AgNP/Mg nanoparticles were synthesized using the Melanoleuca grammopodia mushroom. Vitamin C and E contents of the mushroom were 42.6 mg/g and 26.82 mg/g, respectively. FTIR analysis revealed the reducing and stabilizing effects of bioactive components present in M. grammopodia mushroom extract. SEM micrographs showed that the nanoparticles were spherical in form and the EDX spectrum confirmed the presence of the characteristic metallic silver peak at 3.0 keV. A significant reduction in …

Design And Structural Optimization Of Acceleration Pipe For Mhd Accelerated Jujube Air-Suction Harvester, Yi Wang, Jing Nie, Jingbin Li, Longpeng Ding, Xuewei Chao, Xianfei Wang

Turkish Journal of Agriculture and Forestry

The combination of magnetohydrodynamic (MHD) acceleration technology and the negative pressure suction principle ensures the effective lifting and transport of jujube fruits, addressing the challenges of traditional harvesting methods and providing a new solution for improving jujube harvesting efficiency. This study focuses on the impact of key parameters, including pipe diameter, electrode plate length, and electrode positioning, on the performance of the MHD acceleration pipe through numerical simulations. The structural optimization of the MHD acceleration pipe in the air-suction jujube harvester aims to enhance airflow acceleration efficiency. The results from both numerical simulations and experimental studies indicate that: 1) a …

Insights From Multivariate Analysis Of Seed Vigor Traits In Rapeseed And Mustard (Brassica Spp.) Genotypes, Ayushi Yadav, Shiv Kumar Yadav, Ravish Choudhary, Sushma Mudlupura Kumar, Anbalagan Anumantharaj, Rakesh Bharadwaj, Rashmi Yadav, Yashpal Taak, Navinder Saini, Sneha Adhikari, Sangita Yadav

Turkish Journal of Agriculture and Forestry

Rapeseed and mustard (Brassica spp.) are critical oilseed crops contributing significantly to global edible oil production. In India, Indian mustard (Brassica juncea) dominates Brassica cultivation, yet the country remains heavily dependent on edible oil imports due to insufficient domestic production. Enhancing seed vigor, a key determinant of crop establishment and yield, offers a promising avenue for improving productivity. This study assessed the genetic diversity of 300 rapeseed and mustard genotypes for seed vigor traits using multivariate analysis, including principal component analysis (PCA) and cluster analysis. Eleven seed vigor-related traits were evaluated, and PCA revealed four principal components with eigenvalues greater …

Effects Of Polyethylene Films With Different Uv-A Transmittance On Quality Parameters And Physiology Of Greenhouse-Grown Tomato, Nefi̇se Yasemi̇n Tezcan, Mustafa Erkan, Ahmet Kurunç

Turkish Journal of Agriculture and Forestry

In this study, the effects of three different polyethylene (PE) greenhouse covering materials (CMs) with varying thicknesses and ultraviolet-A (UV-A) transmittance on greenhouse microclimate, plant growth, yield, fruit quality, and chlorophyll fluorescence parameters (Chl FPs) in tomatoes were investigated. The CMs used in the study were 180 μm thick ultraviolet (UV) + ethylene vinyl acetate (EVA) + long-wave infrared (IR) + light diffuser (LD) + antifog (AF) added PE (CM1), 160 μm thick PLUS UV + EVA + IR + LD + AF added PE (CM2), and 200 μm thick UV + EVA + IR + LD added PE (CM3). …

Wbd-Yolo-Am: Yolov8 With Attention Module And Iot-Based Wild Boar Detection And Deterrence System For Safeguarding Small Millets, Aditya Joshi, Neha Pandey, Manoj Diwakar, Prabhishek Singh, Achyut Shankar, Fayez Alqahtani

Turkish Journal of Agriculture and Forestry

Small millets are traditional and climate-resilient food crops that can grow in adverse weather conditions. Animal intrusion, specifically from wild boars, is a main threat to the production of small millets, which reduces interest in the cultivation of small millets and affects farmers’ incomes. To address the problem of wild boar attack on agriculture land, an improved YOLOv8 is proposed, with an attention module based on WBD-YOLO-AM and that works from the collection of data, its preprocessing, attention mechanism to improve the feature maps, training then building the model, after that the models hyperparameter are tuned to boost the performance …

The Status Of Agritourism In Bosnia And Herzegovina, Nerma Čolaković-Prguda, Jasna Hasanbegović, Irma Đidelija Čolaković, Lejla Škaljić, Nuray Demi̇r

Turkish Journal of Agriculture and Forestry

Agritourism in Bosnia and Herzegovina has a lot of potential benefit for economic growth and sustainable development in rural regions, as it incorporates local culture and natural beauty into the tourism offerings of the region. Tourism creates opportunities to diversify the economy, retain traditions, improve local income, and protect natural resources. This study covered Međugorje, Ilijaš, Rostovo, Vlašić, Bosanska Krupa, Neum, Olovo, Gornji Vakuf, Sarajevo, and Donji Vakuf. Agritourism can provide authentic experiences like learning about traditional agriculture, fruit harvesting, tasting local products, and environmental preservation in destinations like Međugorje and Neum. Ilijaš and Gornji Vakuf have potential in organic …

Innovative Approaches To Estimating Sessile Oak Stem Volume: Comparing Additive Models And Ensemble Machine Learning, Maria J. Diamantopoulou, Abbas Şahi̇n, Ramazan Özçeli̇k

Turkish Journal of Agriculture and Forestry

Sessile oak is an important deciduous forest tree species across Türkiye and Europe. Estimating individual tree and stand volumes is essential for growth and yield models, as these predictions play a critical role in assessing forest management, economics, and fire risk. In pursuit of this goal, nonlinear seemingly unrelated regression (NSUR) was used for the simultaneous fitting of stem volume components, and compared to three ensemble machine learning techniques: gradient boosting regression (GBr), XGBoost regression (XGBr), and random forest regression (RFr). NSUR accounts for correlations between error terms across different equations, leading to more efficient parameter estimates than if each …

Tracing Priming Effects In Palsa Peat Carbon Dynamics Using A Stable Isotope-Assisted Metabolomics Approach, Christian Ayala-Ortiz, Moira Hough, Elizabeth K. Eder, David W. Hoyt, Rosalie K. Chu, Jason Toyoda, Steven J. Blazewicz, Patrick M. Crill, Ruth Varner, Scott R. Saleska, Virginia I. Rich, Malak M. Tfaily

Michigan Tech Publications

Introduction: Peatlands store up to a third of global soil carbon, and in high latitudes their litter inputs are increasing and changing in composition under climate change. Although litter significantly influences peatland carbon and nutrient dynamics by changing the overall lability of peatland organic matter, the physicochemical mechanisms of this impact—and thus its full scope—remain poorly understood. Methods: We applied multimodal metabolomics (UPLC-HRMS, ¹H NMR) paired with ¹³C Stable Isotope-Assisted Metabolomics (SIAM) to track litter carbon and its potential priming effects on both existing soil organic matter and carbon gas emissions. Through this approach, we achieved molecule-specific tracking …

Early Detection Of Oak Wilt Using Unmanned Aerial Vehicles (Uav) & Computer Vision, Muttaki I. Bismoy

Masters Theses

Forests are critical ecosystems, delivering services such as biodiversity conservation, climate regulation, timber production, and recreation. However, they face increasing threats from pathogens like Bretziella fagacearum, which causes Oak Wilt, a lethal disease that disrupts water transport in oak trees, leading to canopy dieback and eventual death. Traditional detection methods rely on manual ground surveys, which are labor-intensive, time-consuming, and prone to error, particularly in early disease stages.

This research presents an automated, scalable, high-precision Oak Wilt detection system using Unmanned Aerial Vehicles (UAVs) combined with deep learning-based computer vision. Expanding on earlier work with a lightweight CNN achieving an …

Periodic Evaluations Indicate Increased Effectiveness Of Oregon Master Woodland Manager Volunteer Program, Alicia Christiansen, Lauren Grand, Norma Kline, Tiffany Hopkins

Journal of Extension

The Oregon State University Extension Service Master Woodland Manager (MWM) volunteer program trains landowners in core forestry and natural resource topics. Landowners participate in the MWM program to increase their forest management knowledge, improve their own management practices, and prepare them to volunteer in their community (Bowers, 2000). We surveyed MWM volunteers to understand the effectiveness of the program and what barriers exist to volunteering. Volunteers find the program to be increasingly adequate for personal management and answering questions, but time continues to be the biggest barrier for completing required volunteer hours.

Northern Arizona Ecological Conservation: Assessing Quaking Aspen Health In Northern Arizona Using Earth Observations, Ikram Morso, Luke Collins, Margaret Cox, Melissa Schwan

Aspen Bibliography

Quaking aspen (Populus tremuloides) forests in Northern Arizona provide critical habitat for endangered bird species and support regional tourism and recreation. However, aspen populations are declining due to a combination of abiotic and biotic stressors. In response, land managers implemented strategies, such as ungulate exclusion fencing, prescribed burns, and stand thinning, but efforts are constrained by limited data on where and when to intervene. This project worked in collaboration with the U.S. Forest Service, the National Park Service, the Arizona Department of Forestry and Fire Management, and Northern Arizona University School of Forestry. The team utilized Earth observations …

Modeling Diameter Distribution Of Loblolly Pine Plantations In Response To Mid-Rotation Thinning Treatments And Assing The Economic Returns In Western Gulf Coastal Plain, Pragya Rijal, Yuhui Weng, Anusha Shrestha, Jason Grogan

Electronic Theses and Dissertations

The influence of thinning regimes incorporating row thinning on diameter distribution and timber product allocation is of considerable importance in the management of loblolly pine (Pinus taeda) plantations within the Western Gulf Coastal Plain. This study utilized data from a thinning study plots established by the East Texas Pine Plantation Research Project across East Texas and Western Louisiana. A two-parameter Weibull distribution was used to model diameter distributions under varying thinning intensities, with parameter estimation conducted using both parameter prediction (PPM) and percentile-based (PRM) methods. The PRM approach demonstrated excellent model performance, with coefficients of determination (R²) ranging …

Enumerating Escherichia Coli (E. Coli) Concentrations And Evaluating Optical Brighteners As A Potential Indicator Of Human Wastewater Contamination In La Nana Bayou, Banita Creek, And Their Tributaries, Nacogdoches, Texas, Swastika Acharya, Matthew Mcbroom, Yuhui Weng, Yanli Zhang

Electronic Theses and Dissertations

La Nana Bayou was added to the Texas 303(d) list in 2000 after it failed to meet water quality standards set by the Texas Commission on Environmental Quality (TCEQ) for primary contact recreation due to elevated Escherichia coli (E. coli) levels. E. coli is a bacterial species commonly found in the intestines of humans and warm-blooded animals. It serves as an indicator of fecal contamination in freshwater, though its presence alone cannot identify the contamination source. Optical brighteners (OBs), chemicals found in laundry detergents, cleaning agents, toilet papers, and clothing, are unique to human wastewater. This study aimed …

Restoration At The Intersection Of Science And Outreach-The Working Woods Learning Forest, Katharine L. Stuble, David J. Burke, Samuel C. Harbol, Chad Knisely, Jessica Miller Mecaskey, Alexa S. Wagner, Michael Watson, Kevin E. Mueller

Biological, Geological, and Environmental Faculty Publications

Botanical gardens and arboreta have enormous (if often under-realized) potential to increase understanding of restoration as a predictive science while simultaneously serving as platforms promoting large-scale restoration by public and private stakeholders. Public gardens often have deep histories of both research and outreach and, increasingly, engagement in restoration efforts. As a team of researchers and practitioners, we share our experiences using a forest management manipulation at the Holden Arboretum for both research and outreach. Forest management is a key tool for enhancing biodiversity and ecosystem services in young forests. Known as the Working Woods Learning Forest, this restoration simultaneously serves …

Breeding Season Bird Use Of And Non-Native Plants Found In Food Plots In The Southern Appalachian Mountains, Nathan A. Klaus, Kirk Stodola

Georgia Journal of Science

Traditional agricultural style food plots, or wildlife openings, are widely used for wildlife management and hunting opportunities on public and private lands in the Southeastern United States. These habitats are often cited as beneficial for game and nongame wildlife, but there are few published studies examining nongame bird use of food plots in recent decades. Modern concerns about the ecological costs of food plots, particularly fragmentation and invasive non-native species, warrant a new examination of this practice. We conducted avian surveys during the breeding season of 2008 at 39 sites composed of three different treatments: traditional food plots, novel food …

Hillslope Flow Paths In Snowmelt And Rainfall Seasons In Permafrost-Underlain Areas, Northeastern Tibetan Plateau: Investigation Based On Hydrochemical Tracers And End-Member Mixing Analysis, Xiong Xiao, Fan Zhang, Fengjing Liu, Xiaoyan Li, Xiaonan Shi, Chen Zeng, Tao Che

Michigan Tech Publications

Study area: The Binggou and adjacent Yakou catchments in the northeastern Tibetan Plateau. Study focus: Hillslope flow paths were studied using hydrochemical data of various water types in the spring snowmelt and summer rainfall periods based on hydrochemical tracers and end-member mixing analysis. New hydrological insights for the study region: End-member mixing analysis confirmed the dominance of surface and near-surface runoff during the spring snowmelt. Specifically, the spring Binggou stream water had 61 % surface runoff, 22 % shallow groundwater, and 17 % near-surface runoff. The spring Yakou stream water had 64 % snowmelt, 25.5 % near-surface runoff, and 10.5 …

Ecological And Social Resilience In Wildfire-Prone Communities, Melissa Burnett

All Theses

Climate projections suggest extreme weather variability, introducing extremely wet years, followed by years with extreme drought. These weather conditions are creating fire weather conducive to increasing wildfires across the southeast over the next century, which can have extreme ecological consequences. The Appalachian Mountains historically had some of the highest fire frequencies in North America but have been subjected to decades of fire suppression and prevention policies, resulting in an environment subject to more high-severity wildfires. The fall of 2016 was one of the driest seasons on record in this region and resulted in intense and destructive fires in the southeastern …

Evaluation Of A Forest Restoration Strategy On Small Mammals In Southern Pine Ecosystems, Gage R. Grantham, Jessica L. Glasscock, Christopher M. Schalk, Kathryn R. Kiid, Reuber Antoniazzi

Electronic Theses and Dissertations

Industrial forestry replanted historic native pine forests (e.g., longleaf pine [Pinus palustris] and shortleaf pine [Pinus echinata]) to homogenous, high-density plantations consisting of native off-site species (e.g., loblolly pine [Pinus taeda]) after intensive harvesting for wood products. The contemporary forest structure that remains, consisting of off-site overstory compositions, can potentially serve as an ecological bridge to facilitate a gradual transition to a more desirable canopy species, thereby conserving biodiversity and ecosystem function. Understanding disturbance-sensitive small mammal communities from restoration strategies (retention) can help achieve long-term management goals for ecosystem conservation across larger scales. I …

An Evaluation Of Key Components Of Natural Resource Camps, Undergraduate Persistence And Success In Natural Resources, Brandy Bishop

Electronic Theses and Dissertations

This study explores the key components that connect natural resource camps, undergraduate persistence, and overall success through gainful employment within the natural resource industry. The aim of this study was to assess early interest and attitudes towards natural resource career pathways and how education interventions shape students’ career outcomes thus leading to overall success. A longitudinal mixed method design was used combining Conservation Careers Camp survey data (n = 39) with institutional records for Forestry majors (n =655) in the Arthur Temple College of Forestry and Agriculture on the Stephen F. Austin State University campus. Quantitative data was analyzed through …

The Effect Of An Invasive Species (Lantana Camara) And A Fast-Growing Herb (Acanthus Pubescens) On The Plant And Animal Communities In Kibale National Park, Uganda, Kathleen Bufka

Theses and Dissertations

Fast-growing plant species, like Lantana camara and Acanthus pubescens, could threaten biodiversity in Kibale National Park, Uganda. I surveyed plants and wildlife in areas where these plants were frequently encountered compared to those areas where they are infrequent. I found mixed results based on plant traits and the animals considered. My findings support management efforts on these two species to prevent forest degradation.

Stand Strucuture Of Planted Stands In Storm-Water Retention Basins, Jason Lanza

Electronic Theses and Dissertations

Trees assist in stormwater retention basin functionality as efficient sources of water removal, and determining stand structure and leaf area of forests can be a measure of their effectiveness. The structure of forest stands on these basins managed for runoff mitigation has received little attention. We investigated the structure and species composition/diversity (tree and herbaceous) of planted, managed stands and naturally regenerated stands on stormwater retention basins managed by Harris County Flood Control District in Harris County, Texas. We determined stand structure variables (trees per hectare, basal area per hectare, quadratic mean diameter, relative density, average tree height, and percent …

Factors Underlying A Latitudinal Gradient In The S/G Lignin Monomer Ratio In Natural Poplar Variants, Jin Zhang, Pradeep Kumar Prabhakar, Yen On Chan, Rachel A. Weber, Mengjun Shu, Ganesh Panzade, Connor J. Cooper, Russell B. Davidson, Jin-Gui Chen, Sara S. Jawdy, Jerry M. Parks, Breeanna R. Urbanowicz, Gerald A. Tuskan, Trupti Joshi, Richard A. Dixon, Wellington Muchero, Jaime Barros

Biomedical Sciences

The chemical composition of wood plays a pivotal role in the adaptability and structural integrity of trees. However, few studies have investigated the environmental factors that determine lignin composition and its biological significance in plants. Here, we examined the lignin syringyl-to-guaiacyl (S/G) ratio in members of a Populus trichocarpa population sourced from their native habitat and conducted a genome wide association study to identify genes linked to lignin formation. Our results revealed many significant associations, suggesting that lignin biosynthesis is a complex polygenic trait. Additionally, we found an increase in the S/G ratio from northern to southern geographic origin of …

Restoring A Longleaf Pine Savanna In The Wiregrass Gap: Community Responses To Post-Harvest Slash Manipulation, Eva Purcell

All Theses

Now reduced to just a fraction of their historical range, Longleaf pine savannas in the southeastern United States are home to a wide array of species, some of which are threatened or endemic. These characteristics have inspired widespread restoration initiatives in recent decades, accompanied by an expanding literature on how management techniques affect restoration outcomes. However, research is lacking in a unique region of longleaf pine’s range, where the dominant fire-carrying grasses are different: the Wiregrass Gap. This study investigated the effects of three different slash manipulation treatments on restoration outcomes of a Wiregrass Gap longleaf pine savanna. Specifically, we …

Is There A More Effective Design For Quadrat To Increase Ease Of Use And Vegetation Density?, Scott M. Burke Jr., Kristen M Bellisario

Discovery Undergraduate Interdisciplinary Research Internship

Quadrats are a special tool in the ecology field that allows the measurement of vegetation density for further data analysis of a habitat. The larger the quadrat cross-sectional area, the better the results of the data, which in turn can make the quadrat hard to carry through field sites. In wildlife corridor research, quadrat surveys provide a quantitative metric for habitat suitability. In this work, we consider the efficiency and ease-of-use benefits for field work in the design of a collapsible version of a quadrat using a House of Quality (standard metric for new design requirements) to assess the customer …

Assessment Of The State Forest Protection Management And Community Participation In The Central Highlands, Vietnam, Do Cao Phan, Tran Thi Lan

ASEAN Journal of Community Engagement

This study focuses on state forest protection management in Central Highlands Vietnam, particularly in the Krong Bong District, Dak Lak Province, examining the role of community participation. The Central Highlands, a region rich in biodiversity and ecological significance, is severely impacted by deforestation, illegal logging, and land-use changes. This study assesses the current state of forest governance and proposes sustainable development improvement strategies. The research employs a mixed-methods approach, integrating secondary data, such as government reports and forest management reports from Krong Bong District, Đak Lak Province, as well as previous studies, with primary data collected from surveys of 96 …

Gene Regulatory Network Prediction Using Machine Learning, Deep Learning, And Hybrid Approaches, Sai Teja Mummadi, Md Khairul Islam, Victor Busov, Hairong Wei

Michigan Tech Publications

Construction of gene regulatory networks (GRNs) is essential for elucidating the regulatory mechanisms underlying metabolic pathways, biological processes, and complex traits. In this study, we developed and evaluated machine learning, deep learning, and hybrid approaches for constructing GRNs by integrating prior knowledge and large-scale transcriptomic data from Arabidopsis thaliana, poplar, and maize. Among these, hybrid models that combined convolutional neural networks and machine learning consistently outperformed traditional machine learning and statistical methods, achieving over 95% accuracy on the holdout test datasets. These models not only identified a greater number of known transcription factors regulating the lignin biosynthesis pathway but also …