Entomology Commons^™

New Records Of Biting Midges Of The Genus Culicoides Latreille From The Southeastern United States (Diptera: Ceratopogonidae), Stacey L. Vigil, John C. Wlodkowski, Joshua Parris, Suzanne Edwards De Vargas, David Shaw, Christopher Cleveland, William L. Grogan Jr., Joseph L. Corn

Insecta Mundi

We provide new state and county records of biting midges in the genus Culicoides Latreille (Diptera: Ceratopogonidae) from the southeastern United States collected with CDC miniature light traps during 2007–2012 in Florida, Georgia, Alabama, Mississippi, Louisiana, Arkansas, and Texas. The primary goals of the surveys were to identify the presence of exotic Culicoides, and determine the ranges of known and possible vectors of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV). Included are the first records of: Culicoides (Amossovia) beckae Wirth and Blanton from Louisiana and Mississippi, C. (A.) oklahomensis Khalaf from Alabama and Arkansas, …

La Tribu Phanaeini (Coleoptera, Scarabaeidae, Scarabaeinae) En El Perú / The Tribe Phanaeini (Coleoptera: Scarabaeidae: Scarabaeinae) In Peru, Luis Figueroa, William D. Edmonds, Norberta Martínez Luján

Entomology Papers from Other Sources

La presencia de la tribu Phanaeini (Coleoptera: Scarabaeidae: Scarabaeinae) en el Perú fue revisada en base a la colección del Museo de Historia Natural de la Universidad Nacional Mayor de San Marcos (Lima) y literatura especializada. Cada especie es presentada con citas para su diagnosis y datos de distribución y algunos comentarios. En este estudio se registran 30 especies que pertenecen a diez géneros: Coprophanaeus, Dendropaemon, Gromphas, Diabroctis, Oruscatus, Oxysternon, Phanaeus, Sulcophanaeus, Tetramereia y Megatharsis. La especie Oruscatus davus es la única especie distribuida en la parte alta y occidental de …

Characterizing The Maternally Inherited Endosymbionts Of Solitary Bees, Abiya Saeed

Theses and Dissertations--Entomology

Solitary bees are important pollinators of crops, with species in the family Megachilidae (mason bees) being used for orchard pollination. Commercial movement of these bees also moves their microbiota, including bacterial endosymbionts capable of reproductive manipulation. To test for presence of these bacteria, I screened commercially available species of US orchard pollinators and locally captured solitary bees from Kentucky. I also set up mason bee boxes in five apple orchards to examine recruitment of local pollinators. I conducted 454-pyrosequencing to determine bacterial diversity within four species followed by diagnostic PCR of 30 collected species (184 individuals) to determine infection frequency …

Chasing Tachinids ‘Down Under’: Expeditions Of The Phylogeny Of World Tachinidae Project, Part Ii Eastern Australia, James E. O'Hara, Pierfilippo Cerretti, John O. Stireman Iii, Isaac S. Winkler

Biological Sciences Faculty Publications

Last year we documented in the pages of this newsletter an expedition to the Western Cape of South Africa in search of tachinids for the “Phylogeny of World Tachinidae” project (Cerretti et al. 2013). The project itself was discussed in a separate article (Stireman et al. 2013). In this issue of The Tachinid Times we discuss our expedition to eastern Australia in late 2013. This trip took place so recently that we have yet to fully identify our material and as a result cannot record here all of the taxa we collected. Instead, we will add the taxa to a …

Natural History Notes And New County Records For Ozarkian Millipeds (Arthropoda: Diplododa) From Arkansas, Kansas And Missouri, N. W. Youngsteadt, C. T. Mcallister

Journal of the Arkansas Academy of Science

No abstract provided.

Computational Identification And Molecular Verification Of Mirna In Eastern Subterranean Termites (Reticulitermes Flavipes), Tian Yu

Theses and Dissertations--Entomology

Reticulitermes flavipes is one of the most common termite species in the world, and has been an intriguing research model due to its ecological and biological and economic significance. The fundamental biological question addressed by this study is to elucidate the role of miRNAs in termite development and how miRNA can influence labor division. miRNAs are short non-coding RNAs that have an important role in gene regulation at post-transcriptional level, and can potentially be involved in the regulation of caste polyphenism. Using a computational approach, I identified 167 conserved and 33 novel miRNAs in the dataset. miR-iab-4 and 19 other …

The Prevalence And Effect Of Wolbachia Infection On The Brown Widow Spider (Latrodectus Geometricus), Brittany Dane' Arrington

College of Graduate Studies: Theses & Dissertations

Wolbachia was recently identified in the Brown Widow Spider (Latrodectus geometricus). This symbiont has not previously been found in this genus, therefore the consequences on the host’s population dynamics are unknown. The frequency of endosymbiont infection can provide insight into the population dynamics of the host as this bacteria is known to alter host life history. In my 1^st chapter I determine the prevalence of Wolbachia pipentis infection in in the Brown Widow Spider (Latrodectus geometricus) from five locations in the Southeastern United States. Infection frequency differed significantly among sampling locations, ranging from 20% to …

Chemosensory Receptors In Tsetse Flies Provide Link Between Chemical And Behavioural Ecology, Daniel Masiga, George Obiero, Rosaline Macharia, Paul Mireji, Alan Christoffels

All Peer-Reviewed Publications

Tsetse flies survive in a variety of environments across tropical Africa, often rising to large numbers, despite their low birth rate of one offspring every seven to nine days. They use olfactory receptors to process chemical signals in their environments to find food, escape from predators, and locate suitable larviposition sites. We discuss the identification of odorant and gustatory receptors in Glossina morsitans morsitans and the role genomics could play in management of nuisance insects. © 2014 Elsevier Ltd.

Storage Of Mung Bean (Vigna Radiata [L.] Wilczek) And Pigeonpea Grains (Cajanus Cajan [L.] Millsp) In Hermetic Triple-Layer Bags Stops Losses Caused By Callosobruchus Maculatus (F.) (Coleoptera: Bruchidae), C. M. Mutungi, H. Affognon, A. W. Njoroge, D. Baributsa, L. L. Murdock

All Peer-Reviewed Publications

Dry mung bean and pigeonpea grains that had sustained some insect damage but fumigated before the start of the experiment were stored in triple-layer hermetic bags (Purdue Improved Crop Storage (PICS™ bags) or woven polypropylene (PP) bags for 6 months. Some of the bags were artificially infested with cowpea bruchid Callosobruchus maculatus (F.) (PICS1, PP1) while others were not (PICS0, PP0). In an additional trial, PP bags containing the grains were treated with Actellic Super® dust before being artificially infested (PP1Ac). Moisture content, live adult C.maculatus count, grain damage, weight loss, and seed germination were determined on a monthly basis. …

Triple Bag Hermetic Storage Delivers A Lethal Punch To Prostephanus Truncatus (Horn) (Coleoptera: Bostrichidae) In Stored Maize, A. W. Njoroge, H. D. Affognon, C. M. Mutungi, J. Manono, P. O. Lamuka, L. L. Murdock

All Peer-Reviewed Publications

Clean shelled maize was stored in woven polypropylene (PP) or triple layer polyethylene (PICS™) bags in the presence of Prostephanus truncatus Horn (PP1; PICS1) or absence of it (PP0; PICS0) at ambient conditions for six months. Moisture content, live insect population, grain damage, weight loss, and germination were analyzed on a monthly basis. Temperature and relative humidity in both PICS- and PP-bag setups were monitored, as well as the oxygen and carbon dioxide levels in the PICS bags. Overall, maize stored in PICS bags had significantly lower (P<0.05) moisture content than maize stored in PP bags. Storage in PICS bag slowed growth of P.truncatus populations and blocked infestation from the surrounding storage environment. On the contrary, PP bags permitted profuse build-up of insect population and cross-infestation. At six months, grain damage was 0.0%, 3.4%, 50.5% and 73.9%, corresponding to weight loss of 0.0%, 2.0%, 36.3%, and 47.7% in PICS0, PICS1, PP0 and PP1, respectively. Germination of maize stored in PP bags dropped from 91.1% to 37.0% and 12.7% in PP0 and PP1, respectively, whereas germination of maize stored in PICS bags dropped only marginally and reached 78.1% and 71.0% in PICS0 and PICS1, respectively, in six months. Without extreme loss of grain viability, a huge decrease in insect infestation, grain damage and weight loss is achieved by storing maize in PICS bag even under P.truncatus endemic conditions. © 2014 Elsevier Ltd.

Aquaporins Are Critical For Provision Of Water During Lactation And Intrauterine Progeny Hydration To Maintain Tsetse Fly Reproductive Success, Joshua B. Benoit, Immo A. Hansen, Geoffrey M. Attardo, Veronika Michalková, Paul O. Mireji, Joel L. Bargul, Lisa L. Drake, Daniel K. Masiga, Serap Aksoy

All Peer-Reviewed Publications

Tsetse flies undergo drastic fluctuations in their water content throughout their adult life history due to events such as blood feeding, dehydration and lactation, an essential feature of the viviparous reproductive biology of tsetse. Aquaporins (AQPs) are transmembrane proteins that allow water and other solutes to permeate through cellular membranes. Here we identify tsetse aquaporin (AQP) genes, examine their expression patterns under different physiological conditions (blood feeding, lactation and stress response) and perform functional analysis of three specific genes utilizing RNA interference (RNAi) gene silencing. Ten putative aquaporins were identified in the Glossina morsitans morsitans (Gmm) genome, two more than …

Quantitative Factors Proposed To Influence The Prevalence Of Canine Tick-Bourne Disease Agents In The United States, Roger W. Stich, Byron L. Blagburn, Dwight D. Bowman, Christopher Carpenter, M. Roberto Cortinas, Sidney A. Ewing, Desmond Foley, Holly Gaff, Graham J. Hickling, R. Ryan Lash

Biological Sciences Faculty Publications

The Companion Animal Parasite Council hosted a meeting to identify quantifiable factors that can influence the prevalence of tick-borne disease agents among dogs in North America. This report summarizes the approach used and the factors identified for further analysis with mathematical models of canine exposure to tick-borne pathogens.

The Role Of The Bacterial Endosymbiont, Arsenophonus, In The Soybean Aphid, Aphis Glycines, Jason A. Wulff

Theses and Dissertations--Entomology

Bacterial endosymbionts can have profound impacts on their host’s ecology. Notably, endosymbionts can protect their hosts against natural enemies and influence host plant interactions. The endosymbiont Candidatus Arsenophonus infects a wide taxonomic range of arthropod hosts, and is suspected of an uncharacterized mutualistic role in hemipterous insects. In the soybean aphid, Aphis glycines, an introduced pest of soybeans in the United States, Arsenophonus is the sole facultative endosymbiont. The focus of this dissertation is to characterize the role of Arsenophonus in the aphid, with an overall emphasis on its impact on aphid management strategies.

I first used diagnostic PCR …

Changing Litter Resources Associated With Hemlock Woolly Adelgid Invasion Affect Benthic Communities In Headwater Streams, Christopher J. Strohm

Theses and Dissertations--Entomology

Hemlock woolly adelgid is an invasive herbivore causing extensive mortality of eastern hemlock, an important foundation species that provides stable conditions influencing biological communities. Hemlock is often found in riparian areas and following its decline, broadleaved species, including birch, beech, and rhododendron, will replace it. These plants differ from hemlock in patterns of canopy cover and leaf properties, which influence conditions and resources within streams.

My goal was to evaluate potential impacts of adelgid-induced alterations to riparian canopies and litter on benthic communities and litter breakdown in streams. I characterized benthic invertebrate communities, litter colonization and litter breakdown in streams …

Exploring The Links Between Seasonal Variation And Spider Foraging, Thomas Edward Dantas Whitney

Theses and Dissertations--Entomology

According to optimal foraging theory, generalist predators, such as spiders, are thought to feed indiscriminately on prey according to its availability, especially when food is scarce. In contrast, generalists can display selective feeding decisions under regimes of high prey abundance, but few studies have tracked changes in prey choice on a seasonal basis under open field conditions. Additionally, adaptations to surviving winter have been largely ignored in the research of foraging behavior. To elucidate this, I monitored prey availability and collected common forest-dwelling wolf spiders for molecular gut-content analysis, in parallel for 18 months, to assess the temporal changes occurring …

Assessing And Mitigating Lawn Insecticide Hazards To Bees And Other Beneficial Invertebrates, Jonathan Lane Larson

Theses and Dissertations--Entomology

Turfgrass settings, including lawns, golf courses, and sports fields, support many beneficial invertebrates that provide important ecosystem services. These non-target organisms and their associated predation, decomposition, and pollination services can be disrupted by the use of certain insecticides. I compared the ecotoxicity of representatives from three major turf insecticide groups, the neonicotinoids, premix formulations, and the anthranilic diamides, in lab and field realistic settings in order to inform industry initiatives towards environmental sustainability.

In lab and field bioassays clothianidin, a neonicotinoid, and a premix clothianidin/pyrethroid spray were acutely toxic to beneficial insects. Populations of predators, springtails, and earthworms, as well …

Insecticide Resistance In The Bed Bug, Jennifer R. Gordon

Theses and Dissertations--Entomology

Populations of Cimex lectularius, the bed bug, have resurged around the world posing significant challenges for pest management professionals and causing physical, economic, and emotional strife. Pyrethroid resistance has been found in the vast majority of populations making pest management more difficult. The objectives of my dissertation research were to document the evolution of resistance to pyrethroid and neonicotinoid combination products (called combination products here) and to a neonicotinoid in the laboratory, to record potential fitness costs to resistance to the combination products, and to compare the efficacy of nine insecticides on six populations. In the laboratory, populations of …

Revision Of The Ant Genus Bothroponera (Hymenoptera: Formicidae: Ponerinae) From The African Continent, Abdulmeneem Joma

Open Access Theses & Dissertations

Abstract (chapter 1)

The Afrotropical ants in the genus Bothroponera include the pumicosa, talpa, and sulcata species complexes. Species in the Bothroponera pumicosa species complex are mainly distributed in the southern part of Africa. This group of ants is characterized by having coarsely foveolate sculpture and by having either a raised a "v" or "u" shaped anterior medial area of the clypeus (anteclypeus), with or without a carina. The mandibles have 7 teeth in the majority of species and 8 teeth in the others. The eyes are relatively large. The scapes usually do not reach the posterior lateral corner of …

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Development Of Fad7-1 Single Mutant Arabidopsis Thaliana Plants That Are Resistant To Aphids, Kaleb L. Vaughn, Carlos A. Avila, Carmen S. Padilla-Marcia, Fiona L. Goggin

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Aphids are a group of sap-feeding insects that attack most of the world’s crops. The loss of function of fatty acid desaturase7 (FAD7) in Solanum lycopersicum (tomato plant) induces aphid resistance that is dependent upon the accumulation of plant defense hormones such as salicylic acid (SA). Tomato lacks most of the genetic resources found in the model plant Arabidopsis (Arabidopsis thaliana). There is an analogous fad7-1 line of Arabidopsis; however, the line has a background mutation, the glabra-1 (gl1), that causes the absence of trichomes (small hairs), which are essential to plant defense. In order to study aphid resistance, a …

An Insight Into The Microbiome Of The Amblyomma Maculatum(Acari: Ixodidae), Khemraj Budachetri, Rebecca E. Browning, Steven W. Adamson, Scot E. Dowd, Chien-Chung Chao, Wei-Mei Ching, Shahid Karim

Faculty Publications

The aim of this study was to survey the bacterial diversity of Amblyomma maculatum Koch, 1844, and characterize its infection with Rickettsia parkeri. Pyrosequencing of the bacterial 16S rRNA was used to determine the total bacterial population in A. maculatum. Pyrosequencing analysis identified Rickettsia in A. maculatum midguts, salivary glands, and saliva, which indicates successful trafficking in the arthropod vector. The identity of Rickettsia spp. was determined based on sequencing the rickettsial outer membrane protein A (rompA) gene. The sequence homology search revealed the presence of R. parkeri, Rickettsia amblyommii, and Rickettsia endosymbiont of …

Interspecific Competition Of A New Invasive Mosquito, Culex Coronator, And Two Container Mosquitoes, Aedes Albopictus And Cx. Quinquefasciatus (Diptera: Culicidae), Across Different Detritus Environments, D. A. Yee, J. F. Skiff

Faculty Publications

The mosquito Culex coronator (Dyar and Knab) (Diptera: Culicidae) has undergone rapid range expansion in the United States since 2003, with its historical distribution in the southwest expanding eastward to the Atlantic coast. Although Cx. coronator nominally use small natural aquatic habitats for development, the use of containers (e.g., tires) makes it potentially important as container invasive. To determine the potential ecological effects of Cx. coronator on resident container species, we conducted a laboratory experiment to assess its competitive ability with two common tire-inhabiting species, Aedes albopictus (Skuse) and Culex quinquefasciatus (Say) (Diptera: Culicidae). Larvae were reared under a factorial …

Characterization Of Cry1f Resistance In Fall Armyworm, Spodoptera Frugiperda (J.E. Smith) Obtained From Puerto Rico And Florida, Vikash Dangal

LSU Master's Theses

The fall armyworm (Spodoptera frugiperda (J. E. Smith)) is a major pest targeted by transgenic corn expressing Bacillus thuringiensis (Bt) proteins in both North and South America. The objective of this study was to characterize the inheritance and fitness costs of the Cry1F resistance in two fall armyworm populations collected from Puerto Rico (RR-PR) and Florida (RR-FL). To determine the inheritance of the resistance, besides RR-PR, RR-FL, and a Cry1F-susceptible population (Bt-SS), 14 other populations were developed by reciprocal crosses, F1 by F1 crosses, backcrosses, and crosses between RR-PR and RR-FL. Diet-incorporated bioassays were conducted to determine the Cry1F susceptibility …

Evaluation Of Economic Injury Levels And Chemical Control Recommendations For Rice Stink Bug (Oebalus Pugnax) In Louisiana, Bryce Blackman

LSU Doctoral Dissertations

Experiments were carried out from 2010 to 2013 to investigate multiple aspects of rice stink bug (RSB) integrated pest management (IPM): insecticide recommendations, sampling efficiency, treatment thresholds, farmer practices, and improved educational tools. The first objective of this research was to compare the efficacies of currently used insecticides with that of a new product from the neonicotinoid class of insecticides. Experiments were carried out in the laboratory, small field plots, and commercial fields across Louisiana. Results from efficacy trials showed that the neonicotinoid was comparable to pyrethroid products used most by rice producers in Louisiana. Conversely, in separate experiments the …

Research In Biological Control Of The Formosan Subterranean Termite, Cai Wang

LSU Doctoral Dissertations

The Formosan subterranean termite, Coptotermes formosanus Shiraki, is a worldwide distributed pest of wooden structures and living plants that causes huge economic losses. Compared to chemical pesticides, biological control may provide a more environmentally friendly and persistent method for the control of C. formosanus. In this research, a series of studies were conducted to understand the termite-pathogen interaction and to develop a feasible biological control strategy. In the first part of the research, the toxicity of Bt toxins expressed by genetically modified maize to termites was tested. Plant tissues or extracts of three commercially planted Bt maize and two non-Bt …

A Revision And Phylogenetic Analysis Of The Genus Eutyphlus Leconte (Coleoptera: Staphylinidae: Pselaphinae) With A Comparison Of Sampling Methodologies, Brittany Elin Owens

LSU Master's Theses

My thesis research includes three components: (1) a methodology project, (2) a systematic project comprising a taxonomic revision, and (3) a morphology-based, cladistic analysis. 1. The methodological study compared the Winkler (Moczarski) eclector and the Berlese-Tullgren funnel (referred to hereafter as “Winkler” and “Berlese” funnels, respectively) as methods for extracting forest litter-dwelling Coleoptera from sifted substrate, with a focus on relevance for taxonomic studies. A general review of the history and methodologies of leaf litter arthropod extraction was provided, in conjunction with repeated, timed trials to compare the relative effectiveness of the two methods using litter samples from various geographic …

Evaluation Of Pyramided Bt Corn For Management Of Corn Earworm And Fall Armyworm (Lepidoptera: Noctuidae), Fei Yang

LSU Doctoral Dissertations

The fall armyworm (FAW), Spodoptera frugiperda, and corn earworm (CEW), Helicoverpa zea, are two target pests of pyramided Bt corn in the U.S. This study determined the susceptibility of the two pests to pyramided Bt corn and evaluated if a 95:5% seed mixture of Bt and non-Bt corn was an appropriate approach for providing refuge populations of CEW for resistance management. Firstly, susceptibility of 150 F2 two-parent families to three common pyramided Bt corn traits was examined using 7-day leaf tissue bioassays and whole plant tests. A few families survived the 7-day leaf tissue bioassays but progeny of the survivors …

The Introduced Dirt-Colored Seed Bug, Megalonotus Sabulicola (Hemiptera: Rhyparochromidae): New For Arkansas, S. W. Chordas Iii, C. T. Mcallister, H. W. Robison

Journal of the Arkansas Academy of Science

No abstract provided.

New Host And Location Record For The Bat Bug Cimex Adjunctus Barber 1939, With A Summary Of Previous Records, M. E. Grilliot, J. L. Hunt, C. G. Sims, C. E. Comer

Journal of the Arkansas Academy of Science

In June 2009, 14 Rafinesque’s big-eared bats(Corynorhinus rafinesquii) were collected from an abandoned house near Shepherd, San Jacinto County, Texas. Three individuals harbored bat bugs which were subsequently identified as Cimex adjunctus Barber 1939. This is the first record of this species from C. rafinesquii. In August 2013, 10 C. rafinesquii were collected from a maternity colony in Drew County in southeastern Arkansas. Four of the bats harbored bat bugs, which were identified as C. adjunctus. This is the first record of this bat bug from Arkansas. A summary of previous state and host records of the insect is provided, …

Phenology Of A Relict Population Of The Pitcher Plant Mosquito, Wyeomyia Smithii (Diptera: Culicidae), In Tattnall Co., Ga, Rachel L. Morreale

College of Graduate Studies: Theses & Dissertations

Wyeomyia smithii Coquillett lives only within the pitchers of the purple pitcher plant, Sarracenia purpurea L. An isolated population of Wy. smithii occurs in Tattnall Co., GA isolated from other populations by over 300 km in all directions. In order to monitor the stability, phenology, and behavior of this population, fluctuations of the larval and pupal abundances (number/pitcher) were monitored over time. The density (number/mL) of Wy. smithii larvae present within pitchers was recorded from June 2012 until April 2014. Fluid volume contained in pitchers had a strong positive effect on larval abundance within the pitcher (r² = 0.624). …