Entomology Commons^™

Transcriptomic Dissection Of Sexual Differences In Bemisia Tabaci, An Invasive Agricultural Pest Worldwide, Wen Xie, Litao Guo, Xiaoguo Jiao, Nina Yang, Xin Yang, Qingjun Wu, Shaoli Wang, Xuguo Zhou, Youjun Zhang

Entomology Faculty Publications

Sex difference involving chromosomes and gene expression has been extensively documented. In this study, the gender difference in the sweetpotato whitefly Bemisia tabaci was investigated using Illumina-based transcriptomic analysis. Gender-based RNAseq data produced 27 Gb reads, and subsequent de novo assembly generated 93,948 transcripts with a N50 of 1,853 bp. A total of 1,351 differentially expressed genes were identified between male and female B. tabaci, and majority of them were female-biased. Pathway and GO enrichment experiments exhibited a gender-specific expression, including enriched translation in females, and enhanced structural constituent of cuticle in male whiteflies. In addition, a putative transformer2 …

Hexapod Herald - Vol. 26, No. 1, February 2014

Hexapod Herald and Other Entomology Department Newsletters

Content: Welcome - Congratulations - Grants - Publications - Meet an Off-Campus Student - Faculty News - Bruner Club News - Blasts from the Past - Condolences (Dr. John (Jack) Campbell) - Retirement Receptions

Nebline, February 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Garbology Uses Science to Teach Environmental Responsibility

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Comparison Of Food-Based Attractants For Bactrocera Invadens (Diptera: Tephritidae) And Evaluation Of Mazoferm - Spinosad Bait Spray For Field Suppression In Mango, Sunday Ekesi, Samira Mohamed, Chrysantus M. Tanga

All Peer-Reviewed Publications

Catches of Bactrocera invadens Drew, Tsuruta, & White (Diptera: Tephritidae) in Multi-lure traps baited with six commercial food-based attractants, Mazoferm E802, Torula yeast, GF-120, Hymlure, Biolure, and Nulure were compared in mango orchards in Kenya. In 2007, Mazoferm E802 and Torula yeast were the most effective attractants and captured 2.4-2.6 times more females and 3.4-4.0 times more males than the standard Nulure. All attractants captured more females than males (ranging from 63 to 74%). In 2008, Mazoferm E802 was the most effective bait capturing 5.6 and 9.1 times more females and males, respectively, than the standard Nulure. Among all the …

Expression Profiling In Bemisia Tabaci Under Insecticide Treatment: Indicating The Necessity For Custom Reference Gene Selection, Pei Liang, Yajie Guo, Xuguo Zhou, Xiwu Gao

Entomology Faculty Publications

Finding a suitable reference gene is the key for qRT-PCR analysis. However, none of the reference gene discovered thus far can be utilized universally under various biotic and abiotic experimental conditions. In this study, we further examine the stability of candidate reference genes under a single abiotic factor, insecticide treatment. After being exposed to eight commercially available insecticides, which belong to five different classes, the expression profiles of eight housekeeping genes in the sweetpotato whitefly, Bemisia tabaci, one of the most invasive and destructive pests in the world, were investigated using qRT-PCR analysis. In summary, elongation factor 1α …

Population Variation In And Selection For Resistance To Pyrethroid-Neonicotinoid Insecticides In The Bed Bug, Jennifer R. Gordon, Mark H. Goodman, Michael F. Potter, Kenneth F. Haynes

Entomology Faculty Publications

Pyrethroid resistance in bed bugs, Cimex lectularius, has prompted a change to combination products that include a pyrethroid and a neonicotinoid. Ten populations of bed bugs were challenged with two combination products (Temprid SC and Transport GHP). Susceptibility of these populations varied, with the correlated response of the two products indicating cross resistance. We imposed selection on three populations using label rate Temprid, and then reared progeny from unselected and selected strains. All selected strains were significantly less susceptible to Temprid SC than unselected strains. Temprid selected strains were also less susceptible to Transport. The pyrethroid component of Temprid showed …

Plasmodium Falciparum Infection Increases Anopheles Gambiae Attraction To Nectar Sources And Sugar Uptake, Vincent O. Nyasembe, Peter E.A. Teal, Patrick Sawa, James H. Tumlinson, Christian Borgemeister, Baldwyn Torto

All Peer-Reviewed Publications

Plasmodium parasites are known to manipulate the behavior of their vectors so as to enhance transmission [1-4]. From an evolutionary standpoint, behavior manipulation by the parasite should expose the vector to limited risk of early mortality while ensuring sufficient energy supply for both it and the vector [5, 6]. However, it is unknown whether this vector manipulation also affects vector-plant interaction and sugar uptake. Here, we show that the attraction of Anopheles gambiae s.s. to plant odors increased by 30% and 24% after infection with the oocyst and sporozoite stages of Plasmodium falciparum, respectively, while probing activity increased by 77% …

The Longhorn Beetles (Coleoptera: Cerambycidae) Of Nebraska, Usa: New State Records, A Checklist Of Known Species, And Distribution Maps, Stephen M. Spomer

University of Nebraska State Museum: Entomology Papers

One hundred eighty-three species/subspecies of longhorn beetles are recorded from Nebraska, an increase of 19 species since 1971. The greatest number of recent discoveries have come from sites along the Missouri River, especially Indian Cave State Park in southeastern Nebraska, as well as the Pine Ridge at Chadron, Nebraska.

An Illustrated Id Guide To The Crayfishes Found In Nebraska Native And Non-Native, Steve Schainost

Nebraska Invasive Species Program

There are five crayfishes native to Nebraska. These are:

Orconectes immunis (papershell crayfish)

Orconectes virilis (northern crayfish)

Orconectes neglectus neglectus (ringed crayfish)

Cambarus diogenes (Devil crayfish)

Procambarus gracilis (prairie crayfish)

Three additional non-native crayfishes have also been found here:

Orconectes rusticus (rusty crayfish) [bait tanks and established in two ponds in Omaha]

Procambarus clarkii (red swamp crayfish) [bait tanks]

Procambarus acutus (White River crayfish) [bait tanks]

I will attempt here to provide a photographic guide to field identification, one species per page.

Seasonal Patterns Of Microhabitat Selection By A Sub-Tropical Whip Spider, Phrynus Longipes, In The Luquillo Experimental Forest, Puerto Rico, Caroline A. Curtis, Christopher P. Bloch

Biological Sciences Faculty Publications

Phrynus longipes (Pocock 1894) is a top predator among arboreal invertebrates in the Luquillo Mountains of Puerto Rico, but many aspects of its ecology remain poorly understood. We sampled four of the most abundant tree species in the Luquillo Mountains during the dry and wet seasons of 2008 to evaluate microhabitat preferences of this species. In the dry season, P. longipes occurred significantly less frequently on a palm, Prestoea acuminata var. montana (Arecaceae), than the other tree species. Carapace length and the diameter of the tree on which an individual was found were positively correlated, suggesting competition for substrates. Microhabitat …

Impact Of Impervious Surfaces On Water Quality By Measurement Of Macroinvertebrates In Rock Island And Moline, Illinois, Kara Noonan

Urban Watershed Project

Streams have been degrading in quality since the introduction of impervious surfaces. Water concentrations have been altered as sediment and other pollutants have been introduced from severe erosion from runoff. As the water continues to change, the biotic integrity becomes disturbed. More specifically, aquatic invertebrate populations begin to change. More organisms that can tolerate poor quality streams are thriving as organisms that can only tolerate high quality streams are beginning to run thin. This study strives to find a spatial relationship between impervious surfaces around Rock Island and Moline, Illinois and the water quality within the streams by looking at …

Male-Male Mounting And The Unreliability Of Body Size As A Character For Mate Choice In Male Japanese Beetles (Popillia Japonica Newman), Paul Switzer, Patrick Forsythe, Kipp Kruse

Faculty Research & Creative Activity

Background: Same-sex pairing is common in many animal species. In many insects, same-sex pairing is often thought to be a result of poor sexual discrimination (i.e., a mistake), but few detailed studies of the mechanisms underlying the mistaken pairing have been conducted. Previous studies have found that in the field, a small proportion of Japanese beetle (Popillia japonica) mating pairs consist of two males instead of a male and a female. In the current study, we investigated the relationship between body size, the tendency to mount other males, and the duration of these mounts, in laboratory experiments on male Japanese …

Insects And Humans Share A Complex History, Aldemaro Romero Jr.

Publications and Research

No abstract provided.

Cloning, Expression, Sequence Analysis And Homology Modeling Of The Prolyl Endoprotease From Eurygaster Integriceps Puton, Ravi Chandra Yandamuri, Ranjeeta Gautam, Charles Darkoh, Vanitha Dareddy, Mustapha El-Bouhssini, Beatrice A. Clack

Faculty Publications

Eurygaster integriceps Puton, commonly known as sunn pest, is a major pest of wheat in Northern Africa, the Middle East and Eastern Europe. This insect injects a prolyl endoprotease into the wheat, destroying the gluten. The purpose of this study was to clone the full length cDNA of the sunn pest prolyl endoprotease (spPEP) for expression in E. coli and to compare the amino acid sequence of the enzyme to other known PEPs in both phylogeny and potential tertiary structure. Sequence analysis shows that the 5ꞌ UTR contains several putative transcription factor binding sites for transcription factors known to be …

Effectiveness Of Hand Removal For Small-Scale Management Of Japanese Beetles (Coleoptera: Scarabaeidae), Paul V. Switzer, Ryan M. Cumming

Faculty Research & Creative Activity

Hand removal is often recommended as a method for small-scale control of Japanese beetles (Popillia japonica Newman). In this study, we investigated the effectiveness of daily hand removal for controlling damage by Japanese beetles on grape plants. We also investigated whether the timing of the removal (at 0800, 1400, or 1900 hours, or at all 3 periods) inßuenced the effectiveness of the technique. We found that hand removal signiÞcantly lowered the number of beetles on, and consequently the damage to, grape plants relative to nonremoval controls. Of the single removal treatments, removal of beetles at 1900 hours was most effective, …

Male-Male Mounting And The Unreliability Of Body Size As A Character For Mate Choice In Male Japanese Beetles (Popillia Japonica Newman), Paul V. Switzer, Patrick S. Forsythe, Kipp C. Kruse

Faculty Research & Creative Activity

Background: Same-sex pairing is common in many animal species. In many insects, same-sex pairing is often thought to be a result of poor sexual discrimination (i.e., a mistake), but few detailed studies of the mechanisms underlying the mistaken pairing have been conducted. Previous studies have found that in the field, a small proportion of Japanese beetle (Popillia japonica) mating pairs consist of two males instead of a male and a female. In the current study, we investigated the relationship between body size, the tendency to mount other males, and the duration of these mounts, in laboratory experiments on male Japanese …

Effectiveness Of Hand Removal For Small-Scale Management Of Japanese Beetles (Coleoptera: Scarabaeidae), Paul Switzer, Ryan Cumming

Faculty Research & Creative Activity

Hand removal is often recommended as a method for small-scale control of Japanese beetles (Popillia japonica Newman). In this study, we investigated the effectiveness of daily hand removal for controlling damage by Japanese beetles on grape plants. We also investigated whether the timing of the removal (at 0800, 1400, or 1900 hours, or at all 3 periods) inßuenced the effectiveness of the technique. We found that hand removal signiÞcantly lowered the number of beetles on, and consequently the damage to, grape plants relative to nonremoval controls. Of the single removal treatments, removal of beetles at 1900 hours was most effective, …

Nebline, January 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Youth Serve Their Community By Volunteering

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

Family and Community Education (FCE) Clubs

and other extension news and events

Contributions To An Arthropod Inventory Of Santa Cruz Island, California, Ida Naughton, Michael S. Caterino, Cause Hanna, David Holway

Publications

Arthropods have been understudied on Santa Cruz Island, resulting in an incomplete understanding of these diverse and ecologically important members of island ecosystems. To enhance the current understanding of Santa Cruz Island biodiversity, we sampled arthropods in 2 native plant habitats: island scrub oak (Quercus pacifica) woodland and patches of island morning glory (Calystegia macrostegia ssp. macrostegia). We used 4 standardized sampling techniques to sample arthropods in 16 Q. pacifica woodland plots. We sampled arthropods associated with C. macrostegia by pan trapping within 1 m of blooming morning glory individuals. In total, we sampled over 18,000 …

An Analysis Of Arthropod Interceptions By Aphis-Ppq And Customs And Border Protection In Puerto Rico, David A. Jenkins, Russell F. Mizell, Skip Van Bloem, Stefanie Whitmire, Leyinska Wiscovitch, Crystal Zaleski, Ricardo Goenaga

Publications

USDA Animal Plant Health Inspection Service Plant Protection and Quarantine (APHIS-PPQ) and Customs and Border Protection (CBP) inspect traffic entering the United States for arthropods posing a threat to national agriculture or ecosystems. We analyzed interceptions made by these agencies in Puerto Rico and the U.S. Virgin Islands between October 2006 and December 2009 for patterns with regard to the frequency of interceptions, origins of interceptions, and the taxa intercepted. 6,952 arthropods were intercepted in freight or luggage entering Puerto Rico and the U.S. Virgin Islands from foreign countries and 9,840 arthropods were intercepted from freight or luggage leaving Puerto …

Letter From The Dean, Michael Vayda

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 15 2014, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Identifying Priority Conservation Areas For The American Burying Beetle, Nicrophorus Americanus (Coleoptera: Silphidae), A Habitat Generalist, Jessica D. Jurzenski, Christopher F. Jorgensen, Andy Bishop, Roger Grosse, John Riens, W. Wyatt Hoback

United States Fish and Wildlife Service: Publications

Conservation efforts leading to the recovery of the federally endangered American burying beetle (ABB), Nicrophorus americanus Olivier, have been challenging because of the unknown causes of its decline, difficulty in establishing habitat requirements, and unclear population distribution across the species’ range. Extant populations of this widespread generalist species occur in broadly separated regions of North America with varying habitat characteristics. A habitat suitability model for ABB in the Nebraska Sandhills was developed over the course of 3 years resulting in a final cross-validated spatial model. The succession of models from 2009 to 2011 indicated that most of the predictive variables …

Progress Towards A Molecular Phylogeny Of Tachinidae, Year Two, Isaac S. Winkler, John O. Stireman Iii, John K. Moulton, Pierfilippo Cerretti, Jeremy D. Blaschke

Biological Sciences Faculty Publications

We reported last year (Stireman et al. 2013) in The Tachinid Times on our collaborative project, funded by the U.S. National Science Foundation, to study the phylogeny of Tachinidae on a worldwide scale. Since that report, we have made some significant progress towards our goal of assembling a robust phylogenetic tree for Tachinidae. Notably, Cerretti et al. (in press) completed a phylogenetic study using 135 morphological characters from 180 tachinid genera. Results from this study largely reflect, with some interesting exceptions, what we are finding from preliminary analyses of our molecular data. Although sampling of taxa and genes for the …

Changes In Gene Expression And Viral Titer In Varroa Jacobsoni Mites After A Host Shift Asian To European Honey Bees, Gladys K. Andino Bautista

Open Access Dissertations

Honey bees (Apis mellifera L.) are the most important insects for the pollination of crops and wildflowers. However, they have experienced increasing colony die-offs during the past two decades. Multiple species of parasitic mites have been described that affect honey bees. The most important species in beekeeping belong to the genus Varroa ( Varroa jacobsoniand Varroa destructor). Varroa mite parasitism of honey bees is thought to be the most significant cause of colony mortality worldwide, and mite resistance to active ingredients of acaricides has become common. V. destructor causes direct impacts on bee production as well as …

A Mega-Diverse Water Beetle Genus (Coleoptera: Hydraenidae: Hydraena Kugelann) Commonly Overlooked In Southeast Asia And Its Potential Use For Environmental Biomonitoring, Hendrik Freitag

Biology Faculty Publications

This study summarizes the current knowledge of the water beetle genus Hydraena Kugelann in Southeast Asia. The surprising species diversity and endemism rates in Hydraenopsis Janssens, the only subgenus present in Southeast Asia, are discussed. Data of five published Hydraena surveys from the Philippines and Singapore are used to evaluate species richness and the occurrence of species assemblages that are subject to the presence and quality of forests. Species richness was found to be generally higher in old grown forests. Some species appeared to be confined to near-natural forests. The denomination of indicator species is impeded by adequate sampling data, …

Population Structure, Genetic Variability, And Gene Flow Of The Bean Leaf Beetle, Cerotoma Trifurcata, In The Midwestern United States, Bamphitlhi Tiroesele, Steven R. Skoda, Thomas E. Hunt, Donald J. Lee, Jaime Molina Ochoa, John E. Foster

Department of Entomology: Faculty Publications

Bean leaf beetle, Cerotoma trifurcata (Forster) (Coleoptera: Chrysomelidae), is a common pest of soybean in the Midwest United States. However, there are currently no reports on the genetic variability of C. trifurcata. This study examined 15–30 individuals from 25 sample locations to estimate genetic variability and gene flow within and among C. trifurcata from across the Midwest. Amplified fragment length polymorphism generated 175 markers for analyses. Results from analysis of molecular variance (AMOVA) indicated that the majority of genetic variation was from within samples; only a small amount of the total variation was attributed to the variation among the samples. …

A Negative Effect Of A Pathogen On Its Vector? A Plant Pathogen Increases The Vulnerability Of Its Vector To Attack By Natural Enemies, Camila F. De Oliveira, Elizabeth Y. Long, Deborah L. Finke

Department of Entomology: Faculty Publications

Plant pathogens that are dependent on arthropod vectors for transmission from host to host may enhance their own success by promoting vector survival and/or performance. The effect of pathogens on vectors may be direct or indirect, with indirect effects mediated by increases in host quality or reductions in the vulnerability of vectors to natural enemies. We investigated whether the bird cherry-oat aphid Rhopalosiphum padi, a vector of cereal yellow dwarf virus (CYDV) in wheat, experiences a reduction in rates of attack by the parasitoid wasp Aphidius colemani when actively harboring the plant pathogen. We manipulated the vector status of aphids …

Minute Pirate Bug (Orius Insidiosus Say) Populations On Transgenic And Non-Transgenic Maize Using Different Sampling Techniques, Santiago A. Palizada, Bamphitlhi Tiroesele, Difabachew B. Kondidie, Muhammad Irfan Ullah, Fatima Mustafa, Thomas E. Hunt, Pete L. Clark, Jaime Molina-Ochoa, Steven R. Skoda, John E. Foster

Department of Entomology: Faculty Publications

Field experiments were conducted to evaluate the populations of minute pirate bug [Orius insidiosus (Say)] using visual, sticky cards and destructive sampling techniques in transgenic and non-transgenic maize in three locations in Nebraska (Mead, Clay Center. and Concord) United States of America. during 2007 and 2008 . All sampling methods revealed significant counts of O. insidiosus on CP4 EPSPS maize plus an insecticide application for control of first generation O. nubilalis at R2 (blister) sampling period. Similarly, visual observations of O. insidiosus on Cry1Ab x Cry3Bb1 x CP4 EPSPS maize yielded significantly higher mean adult counts at R2 (blister) …

Deltosoma Thomson, 1864 Of French Guiana (Coleoptera, Cerambycidae), Pierre-Henri Dalens, Jean-Louis Giuglaris

Insecta Mundi

Two new species of Deltosoma Thomson, 1864 are described from French Guiana: D. humeralis sp. nov. and D. fernandezi sp. nov. Deltosoma lacordairei Thomson, 1864 is redescribed based on examination of the holotype. A study of the aedeagi of these three species was conducted. Dorsal habitus photographs of both sexes, ventral photographs of males, and aedeagi photographs are provided.

Deux espèces nouvelles de Deltosoma Thomson, 1864 sont décrites de Guyane: D. humeralis sp. nov. et D. fernandezi sp. nov. Deltosoma lacordairei Thomson, 1864 est redécrit d’après l’holotype. Les édéages des trois espèces sont comparés. Des photographies des faces dorsales des …