Entomology Commons^™

Seasonal Activity Pattern Of Ambrosia Beetles In Southeast Virginia, Umme H. Akter, Sajrim Chowdhury, Deborah A. Waller

Knowledge and Creativity Expo

Ambrosia beetles (Curculionidae: Scolytinae) are wood-boring insects that influence forest ecosystems through fungal symbioses, rapid host colonization, and invasive species dynamics. Despite their ecological importance, seasonal activity patterns remain poorly characterized in southeastern Virginia. This study integrates field trapping and statistical modeling to examine species-specific seasonal activity across two forested sites in Norfolk, Virginia. Beetles were monitored using ethanol and essential oil-baited bottle traps alongside ethanol-soaked bolt traps. Overall abundance peaked in April, indicating strong early-season emergence. Ethanol-baited traps captured 3-7 times more beetles than essential oil traps, and negative binomial generalized linear models confirmed bait type as a significant …

Trapping Ambrosia Beetles (Curculionidae) With Ethanol-Soaked Tree Bolts In Southeastern Virginia., Sajrim Chowdhury, Umme H. Akter, Deborah A. Waller

Knowledge and Creativity Expo

Native and invasive ambrosia beetles infest a wide variety of tree species in southeastern Virginia and cause significant damage. We investigated trapping ambrosia beetles by using ten different tree species as lures. One objective of the study was to determine the most effective lure for each species of ambrosia beetle related to tree species and the diameter of the bolt. Sections of tree limbs of two different diameters and 25cm in length were soaked in ethanol and hung from trees in several natural locations, both near water and away from water. Beetles attracted to the lures bored into the bolts …

Bayesian Multivariate Modelling Of Lone Star (Amblyomma Americanum) Tick Life Stage Abundance And Temporal Trends To Inform Public Health Risk In Virginia, Thabo Lephoto, Henry Mwambi, Oliver Bodhlyera, Holly Gaff

Biological Sciences Faculty Publications

The increasing abundance of ticks poses a growing public health concern due to heightened human exposure to tick bites. The lone star tick (Amblyomma americanum: Ixodida: Ixodidae), a common human-biting species in the United States, has expanded its range in recent years. However, how its different life stages vary across time, habitats, and locations remains insufficiently understood. We analyzed tick abundance data collected in southeastern Virginia between 2009 and 2018, focusing on larval, nymphal, and adult life stages. A Bayesian multivariate modelling framework was used to examine seasonal patterns, habitat effects, spatial variation, and biological links between life stages. Two …

Larval Crowding In Culex Pipiens Generates Cumulative Cascading Effects Under Changing Environmental Conditions, Sengul Talay, Zafer Sakaci, Michele C. Weigle, Bugrahan Regaip Kilinc, Jenah Parman, Holly Gaff, Deniz Sirin, Bulent Alten, Dennis Bente, Sirri Kar

Computer Science Faculty Publications

Density-dependent population regulation is a fundamental driver in the population dynamics of living systems, ensuring a balanced and sustainable maintenance in nature. It has been suggested that the adverse developmental and morphological effect of high larval density in container mosquitoes represents a significant limiting factor for population density, and the need to investigate this possible importance within the framework of field-based principles has been emphasized. This semi-field study, conducted under the natural thermal regime and using a natural population in Turkish Thrace, aimed to examine the effects of non-food-limiting larval density (crowding effect) in the mosquito species, Culex pipiens ( …

Markov Chain Wave Generative Adversarial Network For Bee Bioacoustic Signal Synthesis, Kumudu Samarappuli, Iman Ardekani, Mahsa Mohaghegh, Abdolhossein Sarrafzadeh

Electrical & Computer Engineering Faculty Publications

This paper presents a framework for synthesizing bee bioacoustic signals associated with hive events. While existing approaches like WaveGAN have shown promise in audio generation, they often fail to preserve the subtle temporal and spectral features of bioacoustic signals critical for event-specific classification. The proposed method, MCWaveGAN, extends WaveGAN with a Markov Chain refinement stage, producing synthetic signals that more closely match the distribution of real bioacoustic data. Experimental results show that this method captures signal characteristics more effectively than WaveGAN alone. Furthermore, when integrated into a classifier, synthesized signals improved hive status prediction accuracy. These results highlight the potential …

Herbivore Identity And Species Richness Shape Fruit Chemical And Quality Responses To Foliar Herbivory, Xavier Ozowara, Taylor M. Sloey, Susan R. Whitehead

Biological Sciences Faculty Publications

The cascading consequences of foliar herbivory on fruit chemistry and quality remain relatively underexplored, with most existing studies focusing on the effects of only one or two attackers. In this study, we examine how increasing herbivore species richness during foliar herbivory alters strawberry (Fragaria × ananassa Duch) fruit quality and phenolic chemistry. We conducted a greenhouse experiment with single species feeding and different combinations of feeding by three generalist lepidopteran species: Spodoptera frugiperda, Heliothis virescens, Helicoverpa zea. Species fed on leaves for 72 h as fruits ripened, and fully ripe fruits were harvested 72 h after …

Tick Lipocalin Triggers Mammalian Igfbp-3-Mediated Apoptosis In Macrophages And Keratinocytes, Krittika Nandy, P. P. Mahesh, Lichao Liu, Daniel E. Sonenshine, Hameeda Sultana, Girish Neelakanta

Biological Sciences Faculty Publications

Ticks secrete several molecules, including lipocalins, in their saliva during blood feeding on a vertebrate host. In this study, we provide novel evidence on the role of Ornithodoros turicata americanus tick lipocalin (Otlip) in modulating cytokine expression and in triggering apoptosis in mammalian macrophages and keratinocytes. The cytokine array analysis revealed significantly increased secretion of insulin-like growth factor-binding protein 3 (IGFBP3) from murine macrophage cell line upon treatment with recombinant Otlip protein (rGST-Otlip) when compared to the secretion noted from cells treated with a control protein (rGST). Similar observation with cytokine protein array analysis was noted when murine macrophages were …

02 - Habitat Preference Of Ambrosia Beetles (Curculionidae) Using Bottle Traps In Southeastern Virginia, Sajrim Chowdhury, Morgan Harrison, Deborah A. Waller, Umme Akter

Undergraduate Research Symposium

Ambrosia beetles attack host trees by boring into the stem and infecting the tree with a symbiotic fungus. Infected trees suffer damage to the vascular system which often results in tree mortality. We examined whether different species of native and invasive ambrosia beetles prefer different habitats by placing bottle traps in riparian areas near streams and in forested habitats in several locations in southeastern Virginia. Numerous ambrosia beetles were collected in the traps, and we related ambrosia beetle species to the habitat in which it was collected.

Developmental Regulation Of Corazonin, Eclosion Hormone, And Bursicon Messages And Rnai Suppression Of Corazonin In Adult Female American Dog Ticks, Dermacentor Variabilis, Anirudh Dhammi, Brooke Bissinger, Loganathan Ponnusamy, Daniel E. Sonenshine, R. Michael Roe

Biological Sciences Faculty Publications

The insect molting process is critical to growth and development and is regulated in part by the neuropeptides corazonin, eclosion hormone, and α and β bursicon. We found messages in a synganglion transcriptome from adult, female American dog ticks, Dermacentor variabilis (that do not molt), with a high similarity to the larval insect neuropeptides that control molting. The phylogenetic analysis of the tick putative neuropeptides compared to other arthropods is discussed in detail. The relative gene expression of these peptides was determined by quantitative PCR during the following adult developmental stages: (i) virgin, unfed 0–24 h after entering the adult …

Man With An Itchy Back, Katherine Schaffer, Nevin Adamski, Brittany Ambrose, Kathleen Anderson

Department of Emergency Medicine Faculty Publications

[First paragraph] A 61-year old man with no significant past medical history presented with painful bug bites on his lower back 1 day after a 1.5-week-long visit to Africa. He noticed bug bites on his lower back 3 days ago; since then, they have increased in size and become more painful. He was prescribed doxycycline at urgent care earlier that morning and had taken 1 dose. He is taking prophylactic medications for malaria and has had no fever at home. Vital signs, exquisitely tender papular lesions with a central punctum and surrounding erythema in the bilateral paralumbar regions.

Description Of All Stages Of A New Tick Species From California Haemaphysalis Vespertina (Acari: Ixodidae), With Redescription Of H. Leporispalustris Packard, 1869 Adults And Phylogenetic Relationships Among Related U.S. Taxa, Andrea Egizi, Santiago Nava, Angie Nakano, Megan E. M. Saunders, Lauren P. Maestas, Autumn D. Angelus, Bruce Noden, Robyn M. Nadolny, Waheed I. Bajwa, Charles Lubelcyzk, Chanakya R. Bhosale, Susan Paskewitz, Holly D. Gaff, Lorenza Beati

Biological Sciences Faculty Publications

All active stages of Haemaphysalis vespertina sp. nov. (Acari: Ixodidae), a tick previously identified as H. leporispalustris Packard, 1869, are described from specimens collected on the vegetation and from leporids in California and Oregon. The adults of H. leporispalustris Packard, 1969 are redescribed based on type material. Adults of the two species can be distinguished by their overall size, the dorsal shape of palpal segment II, the number and shape of dorsal and ventral setae on palpal segment II, the number of spurs on coxae II, the length of setae on scutum, legs and coxae, and the pattern of scutal …

Efficacy Of Cloves As A Repellent For Pest Ants (Formicidae), Aadee Malavade

2024 REYES Proceedings

I examined the efficacy of cloves for repellent activity against foraging ants. Control baits consisting of a cracker/paper substrate topped with granulated sugar or chapati (Indian bread) were presented next to treatment baits (cracker/paper, granulated sugar and spice spread over the sugar) to ants in natural habitats. After an hour, the numbers of ants recruiting to the control and treatment baits were recorded. A timed study of ants with 30-minute duration was done to understand behavior of ants over time. Data were analyzed with the sign test. Cloves showed promise as ant repellents.

Ants And Their Response To Chili Powder, Antu Biswas

2024 REYES Proceedings

Spices can be used as an eco-friendly ant repellent. In this study, I tried to observe the ants’ response to chili powder and whether or not the spice can be an effective ant repellent.

The Efficacy Of Dry Peppermint Leaves As An Ant Repellent, Amal Chacko

2024 REYES Proceedings

Ant (Formicidae) infestation is a typical residential disturbance, often necessitating the use of chemical repellents that pose environmental and health dangers. This study looks into the possibilities of a natural alternative, dry peppermint (Mentha piperita L) leaves as a chemical repellent. Given the diversity of ant species and their variable responses to stimuli, this study seeks to determine the efficiency of dried peppermint leaves as a general ant repellent. A pilot study will be carried out to determine the repellent qualities of this spice against a specific ant species. The precise aims include evaluating if dry peppermint leaves …

Ants And Spices: Investigating The Repellent Effects Of Household Spices On Ants: A Study Of Chili, Coriander, And Cumin Powders, Abhishikta Pradhan

2024 REYES Proceedings

The aim of this study was to test the ant-repellent potential of household spices, namely chili powder, coriander powder, and cumin powder, against ants (Formicidae). The experiments were conducted using sticky note paper as a substrate, on which granulated sugar was placed as bait. In the control baits, only granulated sugar was used, while in the treatment baits, granulated sugar mixed with each spice was used. The baits were placed near ant nests. One hour after placing the baits, the number of ants on the control and treatment baits was counted for each control-treatment pair (five pairs in total, with …

Cardamom Spice: A Potential Ant Repellent, Deepanshi Gupta

2024 REYES Proceedings

This novel research has been conducted to identify the efficacy of cardamom powder to repel ants (Formicidae) in the households and analyze the ant behavior towards the spice. The study was done using a bait and a substrate along with a spice. Granulated sugar was used as a bait material with butter crackers as a substrate. The granulated sugar was topped over the substrate in the control sample while the cardamom powder was added on top of the sugar in the treatment sample. The control sample (no cardamom powder) and treatment sample (with cardamom powder) were kept at a distance …

Evaluating The Repellent Properties Of Paprika On Ants, Jonathan J. Toj Castellanos

2024 REYES Proceedings

Ants are a problem no matter where you are, which is why we should always have a way to repel them on hand. Spices can be an eco-friendly and cheaper alternative to common insecticides sold in supermarkets. These insecticides are generally made from toxic products, which can affect both humans and animals. This research aimed to find the perfect spice to replace insecticides. Paprika was chosen because it is very common, especially in Guatemalan territory. Several experiments were conducted to verify the effects of paprika on ants. It was tested on one of the most common ant species in Central …

Investigating Turmeric’S Sustainable Properties For Pest Control As An Ant Repellent, Sabyasachi Parija

2024 REYES Proceedings

Spices have long been known to possess various properties, including their ability to deter pests. This study investigates the potential of turmeric (Curcuma longa) as a natural ant-repellent. By testing the spice in various controlled environments, this research aimed to identify its efficacy compared to untreated baits. The results reveal that turmeric is a promising alternative to chemical pesticides, with significant effects in repelling ants without harm to the ecosystem.

The Ant-Repellant Activity Of Ginger (Zingiber Officinale) And Cardamom (Elettaria Cardamomum), Nevil S

2024 REYES Proceedings

Ants, while beneficial in natural ecosystems, can become pests when they invade human habitats. This study explored the potential of common household spices as a non-toxic alternative to chemical pesticides for ant repellency. Experiments were conducted using powdered ginger and crushed cardamom, which were applied to sugar biscuits (bait) in two ways: sprinkled on top and arranged in a circle around the bait. Ant activity was monitored over time, and data was analyzed using the Sign Test. The results indicated that both ginger and cardamom significantly repelled ants, demonstrating their effectiveness as natural ant deterrents. These findings suggest that spices …

White Pepper Powder Repels Ants, Mobashar Ahmad

2024 REYES Proceedings

In this study, I investigated ant responses to white pepper to see whether the spice can repel foraging ants. I performed several bioassays by placing control and treatment baits. The results demonstrated that white pepper powder significantly reduces ant activity.

Investigations Into The Ant Deterrent Properties Of Asafoetida Ferula Asafoetida And Star Anise Illicium Verum, Chinmayaram K

2024 REYES Proceedings

This study assesses asafoetida and star anise as eco-friendly alternatives to chemical ant deterrents. It involved placing replicates of two bait types, a control (granulated sugar) and a treatment (granulated sugar and spice), on yellow paper substrates at five different ant-infested sites. The efficacy of the spice was evaluated by comparing the ant count on each bait every 10, 40, 60, 120, and 180 minutes. Percentage repellency (PR) was calculated for both the spices, and the data was analysed with the sign test. Further tests used only the treatment bait to evaluate the efficacy of the two varieties of asafoetida …

Natural Enemies Of Varroa Destructor Identified From Eastern North America Honey Bee Colonies: A Biological Survey Of Candidates For Mite Control From Maryland, Usa, Francisco Posada-Flórez, Daniel Sonenshine, Jay Evans, Dawn Boncristiani, Monica Pava-Ripoll, Steven Cook

Biological Sciences Faculty Publications

A survey was conducted of microbes and arthropods associated with Varroa destructor (Acari: Varroidae) mites (hereafter, Varroa) collected from honey bee colonies in Maryland, USA. Live Varroa were challenged with samples of both microbes and arthropods and assessed for their potential as biocontrol agents against the mites. To our knowledge, this is the first survey of natural enemies of Varroa conducted in North American honey bee colonies. The survey revealed 21 fungi, 25 bacteria, one nematode, and 8 arthropod species associated with Varroa in honey bee colonies, and from challenge assays using 11 of these, including 4 species of …

Invasion Of Superbugs: Cockroach-Driven Outbreak Of Multidrug-Resistant Enterobacter In An Icu, Jennifer Hanrahan, Nicholas Schouten, Steven H. Fyffe, Annette Jencson, Stephanie Stroever

Department of Medicine Faculty Publications

Objective: To describe factors in an outbreak of multidrug-resistant Enterobacter cloacae (MRE) in an intensive care unit (ICU) over a 20-month period including the likely contribution of cockroaches to the outbreak.

Design: This is a retrospective descriptive study.

Setting: ICU in an urban hospital.

Patients: All patients admitted to the ICU during the 20-month outbreak period were included in the study.

Interventions: Infection prevention interventions included contact isolation, hand hygiene, dedicated patient equipment, environmental cultures, and pest control.

Results: 25 patients were identified as being colonized or infected with MRE. Relatedness of the outbreak strain and strains found in cockroaches …

Landscape Genetics Of The Gulf Coast Tick, Amblyomma Maculatum, Sara Simmons Benham

Biological Sciences Theses & Dissertations

Connectivity among populations helps to maintain genetic diversity, population stability, and resilience. The Gulf Coast tick, Amblyomma maculatum, is a vector of the pathogen Rickettsia parkeri. Persistence of tick populations with high rates of R. parkeri infection poses health risks to humans and animals. Mitochondrial haplotypes were characterized by sequencing a fragment of the mitochondrial 16S rRNA gene. A comparative study of A. maculatum and Amblyomma americanum was conducted to identify similar and unique patterns between the species within the same region. Next, I compared A. maculatum sites across three different regions of the United States. This work …

Determination Of The Presence Of Rickettsia Spp. And Borrelia Spp. Carried By Tortoise Ticks From Madagascar, Anna Phan

Biological Sciences Theses & Dissertations

Ticks were removed from three species of Malagasy tortoises, Astrochelys yniphora, A. radiata, and Pyxis arachnoides (comprising two subspecies P. a. arachnoides and P. a. oblonga), between 2012 and 2015. The ticks were presumed to be from the genus Amblyomma. Ticks were morphologically identified and then checked molecularly to confirm their classification or identify any ticks that could not be morphologically identified. Molecular identification was done via end-point PCR that amplified tick cytochrome oxidase (CO1) and tick 12S rRNA genes. Ticks were screened via a real-time polymerase chain reaction assay for the presence of Rickettsia spp. …

Trends And Opportunities In Tick-Borne Disease Geography, Catherine A. Lippi, Sadie J. Ryan, Alexis L. White, Holly D. Gaff, Colin J. Carlson

Biological Sciences Faculty Publications

Tick-borne diseases are a growing problem in many parts of the world, and their surveillance and control touch on challenging issues in medical entomology, agricultural health, veterinary medicine, and biosecurity. Spatial approaches can be used to synthesize the data generated by integrative One Health surveillance systems, and help stakeholders, managers, and medical geographers understand the current and future distribution of risk. Here, we performed a systematic review of over 8,000 studies and identified a total of 303 scientific publications that map tick-borne diseases using data on vectors, pathogens, and hosts (including wildlife, livestock, and human cases). We find that the …

Scoping Review Of Distribution Models For Selected Amblyomma Ticks And Rickettsial Group Pathogens, Catherine A. Lippi, Holly D. Gaff, Alexis L. White, Sadie J. Ryan

Biological Sciences Faculty Publications

The rising prevalence of tick-borne diseases in humans in recent decades has called attention to the need for more information on geographic risk for public health planning. Species distribution models (SDMs) are an increasingly utilized method of constructing potential geographic ranges. There are many knowledge gaps in our understanding of risk of exposure to tick-borne pathogens, particularly for those in the rickettsial group. Here, we conducted a systematic scoping review of the SDM literature for rickettsial pathogens and tick vectors in the genus Amblyomma. Of the 174 reviewed articles, only 24 studies used SDMs to estimate the potential extent …

Double Anus In An Ixodes Scapularis Nymph, A Medically Important Tick Vector, Vikas Taank, Frank A. Lattanzio, Hameeda Sultana, Girish Neelakanta

Biological Sciences Faculty Publications

Background: Ixodes scapularis ticks are medically important arthropod vectors that transmit several pathogens to humans. The observations of morphological abnormalities, including nanism, missing leg, extra leg, and gynandromorphism, have been reported in these ticks. In this study, we report the presence of two anuses in a laboratory-reared I. scapularis nymph.

Results: Larval ticks were allowed to feed on mice and to molt to nymphs. Two anuses were observed in one of the freshly molted nymphs. Stereo and scanning electron microscopy confirmed the presence of two anuses in one nymph within a single anal groove.

Conclusions: This report confirms the rare …

Exploring The Niche Of Rickettsia Montanensis (Rickettsiales: Rickettsiaceae) Infection Of The American Dog Tick (Acari: Ixodidae), Using Multiple Species Distribution Model Approaches, Catherine A. Lippi, Holly Gaff, Alexis L. White, Heidi K. St. John, Allen L. Richards, Sadie J. Ryan

Biological Sciences Faculty Publications

The American dog tick, Dermacentor variabilis (Say) (Acari: Ixodidae), is a vector for several human diseasecausing pathogens such as tularemia, Rocky Mountain spotted fever, and the understudied spotted fever group rickettsiae (SFGR) infection caused by Rickettsia montanensis. It is important for public health planning and intervention to understand the distribution of this tick and pathogen encounter risk. Risk is often described in terms of vector distribution, but greatest risk may be concentrated where more vectors are positive for a given pathogen. When assessing species distributions, the choice of modeling framework and spatial layers used to make predictions are important. …

Varroa Destructor Mites Vector And Transmit Pathogenic Honey Bee Viruses Acquired From An Artificial Diet, Francisco Posada-Florez, Eugene V. Ryabov, Matthew C. Heerman, Yanping Chen, Jay D. Evans, Daniel E. Sonenshine, Steven C. Cook

Biological Sciences Faculty Publications

The ectoparasitic mite Varroa destructoris one of the most destructive pests of the honey bee (Apis mellifera) and the primary biotic cause of colony collapse in many regions of the world. These mites inflict physical injury on their honey bee hosts from feeding on host hemolymph and fat body cells/cellular components, and serve as the vector for deadly honey bee viruses, including Deformed wing virus (DWV) and the related Varroa destructor virus-1 (VDV-1) (i.e., DWV-like viruses). Studies focused on elucidating the dynamics of Varroa-mediated vectoring and transmission of DWV-like viruses may be confounded by viruses present in …