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Articles 1 - 30 of 34
Full-Text Articles in Entomology
Evaluating Benthic Macroinvertebrate Communities In Streamside Management Zones In Private, Working Forests, Haley Vaglienti
Evaluating Benthic Macroinvertebrate Communities In Streamside Management Zones In Private, Working Forests, Haley Vaglienti
College of Graduate Studies: Theses & Dissertations
Forestry operations can alter riparian vegetation and increase sediment and nutrient inputs to streams, affecting water quality and aquatic biota. Forestry best management practices (BMPs) including streamside management zones (SMZs) are designed to mitigate those effects by reducing sediment delivery and moderating temperature, flow, and nutrient regimes. We assessed ecological integrity at 24 intermittent stream sites with SMZs in private, working forests across the Piedmont and Southeastern plains of Georgia. At each site, we conducted physical habitat evaluations and characterized benthic macroinvertebrate assemblages. Sites represented a gradient of habitat conditions, SMZ widths, and pine stand ages, allowing for comparisons of …
Preventing Coccus Longulus Infestation, Nicholas Wellls
Preventing Coccus Longulus Infestation, Nicholas Wellls
Honors College Theses
In the Armstrong greenhouse, the Coccus longulus scale insect is a prevalent problem on a specific species of Begonia known as Begonia ‘Richmondensis’. Inside the greenhouse there are several types of begonias with very similar characteristics to the Richmondensis begonia. However, none of these other begonias are infested by C. longulus, despite similar leaf thickness and environmental conditions such as humidity and light. This project examined whether differences in the chemical composition of the Begonia leaves can explain this pattern. Results were not statistically significant, but they showed that the chemicals had a noticeable impact on the scale, and …
Cytonuclear Incompatibilities In Hybrid Systems: Oxidative Phosphorylation Capacity And Nuclear Marker Transmission Ratio Distortion In Hybrids Of An Introgressed Strain Of Parasitoid Wasp, Julie Kessler
College of Graduate Studies: Theses & Dissertations
Speciation is an evolutionary process resulting in the divergence of populations into reproductively isolated species, a key concept that supplies our planet with an abundance of biodiversity. Mechanisms for speciation center on obstacles to fertilization and include post-zygotic barriers, such as intrinsic deleterious interactions, that arise in hybrid systems where genetic variants are combined. Genes from separate parent lineages are often incompatible due to the co-evolution of paired genes within a singular species not matching their complement genes in another species, and the result is a reduction in fitness of the hybrid when these genes come together. The mitochondrial OXPHOS …
Are Blow Flies (Diptera: Calliphoridae) Active Nocturnally In South Georgia?, Breana R. Manning
Are Blow Flies (Diptera: Calliphoridae) Active Nocturnally In South Georgia?, Breana R. Manning
Honors College Theses
Medicolegal forensic entomology uses insect colonization of remains to estimate minimum post-mortem intervals (mPMI). Blow flies (Diptera: Calliphoridae) are among the earliest colonizers, yet their nocturnal activity remains poorly understood. This study aimed to determine whether blow flies are active at night and whether artificial light influences their behavior. Using baited bottle traps in both artificially lit and unlit environments near Georgia Southern University, flies were collected over two trials in fall 2024. Of the 1,843 blow flies collected, only six were captured at night (0.003%), suggesting minimal nocturnal activity. Lucilia coeruleiviridis preferred unlit environments. In contrast, Cochliomyia macellaria was …
Parasitism By The Twisted-Winged Parasite Xenos Peckii (Strepsiptera), And Its Effects On The Ovary Development In Their Host Paper Wasp, Polistes Metricus (Hymenoptera), Alex K. Snyder
College of Graduate Studies: Theses & Dissertations
Strepsiptera are generally described as parasitic castrators as they cause the reproductive death of the hosts. In southeast Georgia, only a handful of paper wasps are hosts for twisted-winged parasites. One such species, Polistes metricus, is parasitized by a twisted-winged parasite, Xenos peckii. Previous studies document that host survivorship at most developmental stages was not largely influenced by the presence of X. peckii. To determine if X. peckii can affect their host’s ability to survive and reproduce, we dissected P. metricus to determine ovary development and the presence of X. peckii parasites. Parasite presence had no effect on the host’s …
Survey And Identification Of Entomopathogenic Fungi In Millipede Species In Coastal Georgia, Kristin L. Campbell Bailey
Survey And Identification Of Entomopathogenic Fungi In Millipede Species In Coastal Georgia, Kristin L. Campbell Bailey
College of Graduate Studies: Theses & Dissertations
Entomopathogenic fungi are well-known, species-specific pathogens of a wide diversity of insects that infect, manipulate, and kill their insect hosts to complete their life cycle. Recently in Savannah, Georgia, millipedes belonging to the genus Eurymerodesmus were observed exhibiting pre-death climbing behaviors associated with pathogenic fungal infection. Dying millipedes summited vegetation and subsequently died in elevated positions along edge habitat transitioning a recreational field and forest. Upon inspection, fungal structures found emerging from dead millipedes anatomically resembled Arthrophaga myriapodina, which is the first documented entomophthoralean fungus to infect millipedes. The goal of this research was to obtain species-level identification of the …
Antimicrobial Activity Of Cat Flea (Ctenocephalides Felis) Gut Proteins On Different Days After Blood Feeding, Dhruva K. Karnik
Antimicrobial Activity Of Cat Flea (Ctenocephalides Felis) Gut Proteins On Different Days After Blood Feeding, Dhruva K. Karnik
Honors College Theses
Cat fleas (Ctenocephalides felis) are a blood-feeding ectoparasitic insect and a common domestic pest found throughout the world. Because of their reliance on host blood, fleas are exposed to blood-borne pathogens; however, the flea gut lumen is a hostile environment for microbial colonization. For example, the gut epithelia differentially express immune genes in response to feeding. In the present study, we measured the antimicrobial activity of gut proteins from cat fleas at different days after feeding (2, 5, 7, and 14). Dissected flea guts were homogenized, passed through a syringe filter, and measured in a protein assay kit. …
Examination Of Greenhouse Conditions That Influence Scale Infestation Rates On Begonia, Madeline M. Cusick
Examination Of Greenhouse Conditions That Influence Scale Infestation Rates On Begonia, Madeline M. Cusick
Honors College Theses
Scale insects are common pests within greenhouse and agricultural settings. I quantified scale infestation on Begonia x Richmondensis, also known as the Dragon Wing begonia, within the Armstrong Campus Greenhouse from March to November of 2023. Six different treatments accounted for variations in sunlight, humidity, and temperature within the greenhouse with the goal of determining the insect’s preferred environmental conditions. Morphological comparisons were also made between host plant Dragon Wing begonia and other begonias present within the greenhouse. Analysis indicated a significant difference in scale presence on replicate plants by zone and sunlight treatments, with more plants hosting at least …
Rescue From Hybrid Mortality In Nasonia Wasps Through An Interspecific Introgression, Samantha K. Mcginn
Rescue From Hybrid Mortality In Nasonia Wasps Through An Interspecific Introgression, Samantha K. Mcginn
Honors College Theses
Nasonia vitripennis and Nasonia giraulti are parasitoid wasps that use fly pupae as hosts to lay their eggs. These two species can produce hybrids when crossed together. However, hybrids are only partially viable and suffer 50-80% mortality from egg to adult development. The highest mortality occurs when N. giraulti females are used in the cross. Previous research shows that hybrids with an N. vitripennis allele of a mitochondrial-targeted gene within a specific locus on chromosome 5 have incompatible interactions with the N. giraulti mitochondria, which are maternally inherited. Collaborators at the University of Rochester have generated an introgression strain of …
Transcriptional Induction Of Immune Pathway Genes And Subsequent Antibacterial Activity In The Digestive Tract Of The Cat Flea (Ctenocephalides Felis)., Katie A. Weber
College of Graduate Studies: Theses & Dissertations
Fleas (Order Siphonaptera) are ectoparasites that serve as vectors of several human diseases (cat scratch disease, flea-borne spotted fever, murine typhus, and plague). Typically, these pathogens are acquired through the ingestion of a host-derived bloodmeal; thus, the lumen of the gut is the first barrier encountered by imbibed pathogens. During the early stages of infection, microbes come into contact with surrounding midgut epithelial cells which trigger a series of host responses to combat local infection. Specifically, pathogen recognition leads to intracellular signaling and an increase in the production of antimicrobial peptides (AMPs) via the IMD and Toll immune pathways. While …
An Assessment Of Functional Characteristics In Macroinvertebrates And Fishes In The Lower Ogeechee River, Molly A. Mckeon
An Assessment Of Functional Characteristics In Macroinvertebrates And Fishes In The Lower Ogeechee River, Molly A. Mckeon
College of Graduate Studies: Theses & Dissertations
The southeastern United States is a hotspot for aquatic biodiversity; yet this region is also considered vulnerable to climate and land–use changes. The Ogeechee River is an unimpeded blackwater river in Georgia that drains into the Atlantic Ocean and a unique ecosystem due to the preservation of its natural state; Historically, research conducted in the river establishes a baseline for long-term ecological studies. The aim of this study is to compare aquatic macroinvertebrate and fish community composition data collected in 2014 to 2017 to a recently collected dataset from 2023 and document temporal changes. Water quality monitoring was performed throughout …
Aggressive And Non-Aggressive Behaviors In Mixed And Non-Mixed Dorymyrmex Smithi And Dorymyrmex Bureni Colonies Of Southeastern Georgia, Usa, Renee K. Nowicki
Aggressive And Non-Aggressive Behaviors In Mixed And Non-Mixed Dorymyrmex Smithi And Dorymyrmex Bureni Colonies Of Southeastern Georgia, Usa, Renee K. Nowicki
College of Graduate Studies: Theses & Dissertations
Dorymyrmex species, ‘pyramid’ or ‘cone’ ants, are Dolichoderines distributed across North America. Two relatively well-studied species, Dorymyrmex smithi and Dorymyrmex bureni, are commonly found throughout southeastern Georgia. These species are part of a socially parasitic relationship while simultaneously having starkly different colony structures. Dorymyrmex smithi is a polydomous, polygynous species with colonies that are often expansive, at times having hundreds to thousands of nests per colony. Dorymyrmex smithi is a temporary social parasite of D. bureni, a monodomous, monogynous species. The specific mechanism of this parasitism is currently unknown. Preliminary behavioral assay data from a limited number of …
Investigating Salinity Intrusion Effects On Sapelo Island, Georgia Using Benthic Macroinvertebrate Bioindicators, Raymond Paul Kidder Ii
Investigating Salinity Intrusion Effects On Sapelo Island, Georgia Using Benthic Macroinvertebrate Bioindicators, Raymond Paul Kidder Ii
College of Graduate Studies: Theses & Dissertations
Coastal wetlands are threatened by the compounding effects of sea level rise and historical land modification. Historically, much of Sapelo Island, Georgia was deforested and drained using a series of ditches and canals to create arable land fit for agriculture and to provide drainage to the inhabited parts of the island. Today, this network of artificial channels is believed to be facilitating the movement of saltwater from the nearby estuary into freshwater areas on the interior of the island. This study aimed to better understand the frequency and magnitude of salinity intrusion events along with their resulting impacts to the …
The Effect Of Larval Competition On Adult Life History Traits And The Immune Response Of Adult Cat Fleas (Ctenocephalides Felis), Piper N. Zellner, Lisa D. Brown
The Effect Of Larval Competition On Adult Life History Traits And The Immune Response Of Adult Cat Fleas (Ctenocephalides Felis), Piper N. Zellner, Lisa D. Brown
Honors College Theses
In holometabolous insects, conditions during the larval stage can affect the adult stage. For instance, in mosquitoes, reduced larval food and high density can make adults more susceptible to infection. Fleas (Siphonaptera), like mosquitoes, go through complete metamorphosis and have adult stages that exclusively feed on the blood of vertebrates, including humans. However, flea larvae have chewing mandibles and rely on nutrients from dried fecal matter containing vertebrate blood proteins. Their limited mobility and unique nutritional needs confine their development to specific areas, likely leading to frequent larval competition in natural conditions. Yet, the influence of larval density on the …
Amblyomma Quadricavum (Schulze, 1941) (Acari:Ixodidae): First Description Of The Nymphal Stage And Phylogenetic Assessment Of Its Taxonomic Status, Ashleigh V. Riggs
Amblyomma Quadricavum (Schulze, 1941) (Acari:Ixodidae): First Description Of The Nymphal Stage And Phylogenetic Assessment Of Its Taxonomic Status, Ashleigh V. Riggs
College of Graduate Studies: Theses & Dissertations
The hard tick (Acari: Ixodidae) Amblyomma quadricavum (Schulze, 1941) is a parasite of snakes and is endemic to the Caribbean region. Morphological characters of this species, namely its rudimentary eye structures, have led to debate about its taxonomic status. The species was originally assigned to the genus Aponomma. However, with revisions of the genus Aponomma, and the recent creation of new genera created for some basal former-Aponomma, it has been suggested that perhaps Amblyomma quadricavum might be a close relative of Robertsicus elaphensis, the Trans-Pecos rat snake tick of the southwest United States and New Mexico, another eyeless former-Aponomma species …
Behavior And Chemical Signals As Markers Of Colony Identification In Argentine Ants (Linepithema Humile), Stephanie A. Rohrbach
Behavior And Chemical Signals As Markers Of Colony Identification In Argentine Ants (Linepithema Humile), Stephanie A. Rohrbach
College of Graduate Studies: Theses & Dissertations
Argentine ants, Linepithema humile, are a highly successful invasive species around the globe and are especially prominent in states such as California and the southeastern United States. L.humile have a unique form of unicoloniality, called “supercolonies”. L. humile can detect colonymates through scent markers in their outer cuticle. With these chemical markers, ants will exhibit high aggression if they smell different from one another. In our study, we performed aggression assays among ten different nest sites and analyzed their CHCs through gas chromatography mass spectrometry, or GC-MS, analysis. For our behavior results, while within-nest interactions displayed low aggression as …
Mortality In Interspecific Hybrids Of Nasonia Vitripennis And Nasonia Giraulti, Bonnie Cobb
Mortality In Interspecific Hybrids Of Nasonia Vitripennis And Nasonia Giraulti, Bonnie Cobb
College of Graduate Studies: Theses & Dissertations
Nasonia is a parasitoid wasp genus that serves as an emerging model for studying speciation due to an incompatibility between nuclear and mitochondrial genomes between sister taxa. Short generation times, easy rearing in a lab setting, producing large amounts of progeny, and whole genome sequencing make Nasonia is an excellent candidate for studying incomplete reproductive isolation. Nasonia have five chromosomes and exhibit haplo-diploid sex determination in which fertilized eggs develop into diploid females and unfertilized eggs develop into haploid males. Recessive phenotypes are hidden in diploid females but are laid bare in haploid males as there is no interaction between …
Investigating The Effect Of A Facultative Symbiont, Hamiltonella Defensa, On Pea Aphid, Acyrthosiphon Pisum, Fecundity And Behavior In Elevated Co₂ Atmospheres, Tyler J. Follman
Investigating The Effect Of A Facultative Symbiont, Hamiltonella Defensa, On Pea Aphid, Acyrthosiphon Pisum, Fecundity And Behavior In Elevated Co₂ Atmospheres, Tyler J. Follman
College of Graduate Studies: Theses & Dissertations
As atmospheric carbon dioxide (CO2) levels continue to rise, it is important to study how economically important organisms, like the pea aphid, Acyrthosiphon pisum, will react to these conditions. The pea aphid feeds on the phloem of crop plants like alfalfa, peas, and fava beans, where it not only directly harms the plant but also can spread plant viruses. A wide variety of factors can influence pea aphid fecundity and behavior. Some of these factors are abiotic, like atmospheric conditions, and some are biotic, like microorganisms with which the pea aphid has a mutualistic relationship. In this thesis, …
High Rates Of Polygyny In Tropical Mexico Within The Native Range Of Vespula Squamosa., Alex K. Snyder, Kevin J. Loope
High Rates Of Polygyny In Tropical Mexico Within The Native Range Of Vespula Squamosa., Alex K. Snyder, Kevin J. Loope
Honors College Theses
Polygyny, or the formation of colonies with multiple cooperating queens, has been observed in a variety of social Hymenoptera and likely exists as a convergent evolutionary strategy. Polygyne cooperation has been observed in several Vespula sp. and is correlated with a perennial social strategy. This perennial-polygyne behavior has been observed most commonly within the tropical and subtropical regions of the invasive Vespula pensylvanica and V. germanica, and rarely within their native temperate ranges. This phenomenon has been relatively undocumented within the tropical portions the V. squamosa native range, despite it being observed in their temperate ranges several times. We observed …
Macroinvertebrate Responses To Hydrological Variation In Experimental Wetlands., Sergio A. Sabat-Bonilla
Macroinvertebrate Responses To Hydrological Variation In Experimental Wetlands., Sergio A. Sabat-Bonilla
College of Graduate Studies: Theses & Dissertations
Predicted increases in the frequency of intense storms and periods of severe drought due to climate change represent a threat to wetland macroinvertebrate communities through alterations to the hydrological regime. I used experimental ponds to assess the effects of water permanence (i.e., duration of flooding) on the communities of aquatic macroinvertebrates. I predicted that permanent ponds would harbor higher diversity of longer-lived taxa whereas temporary ones will favor colonization by quick turnover, short-lived taxa and support lower consumer diversity. Results show differences in macroinvertebrate communities between permanent and temporary ponds can be mostly explained by hydrology and the amount of …
Transcriptional Regulation Of Duox-Dependent Reactive Oxygen Species Production Against Bacterial Infection In The Gut Of Cat Fleas (Ctenocephalides Felis)., Ryne W. Maness
College of Graduate Studies: Theses & Dissertations
Fleas (Order Siphonaptera) are opportunistic blood feeders that parasitize a wide variety of mammals and birds. They also transmit bacterial pathogens that cause diseases in humans (e.g., murine typhus, flea-borne spotted fever, cat scratch disease, and plague). Because they acquire infectious pathogens while blood feeding, the flea gut is considered to be the initial site of infection. While immune responses have been well documented in other disease vectors, few studies have identified the immune mechanisms involved in defense of the flea gut. In other hematophagous insects, the synthesis of reactive oxygen species (ROS) is the immediate immune defense mechanism against …
Inferring Food Web Structure To Identify Seasonal And Longitudinal Patterns In Ogeechee River Invertebrate Communities, Julien Marc Buchbinder
Inferring Food Web Structure To Identify Seasonal And Longitudinal Patterns In Ogeechee River Invertebrate Communities, Julien Marc Buchbinder
College of Graduate Studies: Theses & Dissertations
Understanding how the structure and function of aquatic communities vary across space and time is essential for proper management of freshwater ecosystems. Current management relies on rapid biomonitoring using metrics of community structure, but metrics that incorporate ecosystem processes and functions are only just beginning to see use in assessment and management. Food webs inferred from known species interactions have been proposed as a method of incorporating function into bioassessment without expending extra effort or sacrificing the cost-effectiveness of current monitoring schemes. To apply food webs in biomonitoring, it is first necessary to understand how communities and food webs vary …
Effects Of Climate Variability: A Long-Term Perspective On Leaf Litter Processing In The Ogeechee River, Jose A. Sanchez
Effects Of Climate Variability: A Long-Term Perspective On Leaf Litter Processing In The Ogeechee River, Jose A. Sanchez
College of Graduate Studies: Theses & Dissertations
The Intergovernmental Panel for Climate Change (IPCC) predicts a rise in temperatures and changes to precipitation patterns for the 21st century. These changes are expected to lead to a higher frequency of extreme events such as drought and floods which lead to the alteration of the hydrology, communities, and processes of freshwater ecosystems. Leaf litter decomposition in stream ecosystems is an important component of the energy and nutrient cycle representing a food source for aquatic organisms. This has made it a tool for assessing long term responses to disturbance due to changes in the assemblage of macroinvertebrates that …
Investigating The Role Of Coyotes, Canis Latrans, In The Spread Of Parasites And Arthropod-Borne Diseases In Georgia, Usa., Ansleigh Banks
Investigating The Role Of Coyotes, Canis Latrans, In The Spread Of Parasites And Arthropod-Borne Diseases In Georgia, Usa., Ansleigh Banks
College of Graduate Studies: Theses & Dissertations
In order to analyze the role of coyotes, Canis latrans, as hosts of parasites and arthropod-borne diseases in the state of Georgia, USA, 38 coyotes representing 18 counties and multiple geographic regions of the state were dissected and analyzed for the presence of Dirofilaria immitis, intestinal parasites, ectoparasites, and tick-borne illnesses. Mosquitoes from the locations which the coyotes originated were trapped and analyzed for the presence of D. immitis larvae. In compliance with IACUC guidelines, coyotes were obtained from individuals who had previously hunted or trapped the coyotes for leisure. Parasites were identified based on morphological features with the use …
Morphological Comparison Of Fourth-Instar Larvae From Various Populations Of Wyeomyia Smithii, Kendra D. Moore
Morphological Comparison Of Fourth-Instar Larvae From Various Populations Of Wyeomyia Smithii, Kendra D. Moore
Honors College Theses
Populations of pitcher plant mosquitoes, Wyeomyia smithii, have exhibited recent shifts in blood-feeding behavior, likely in response to changes in climate and food availability. A comparison of various morphological structures in fourth-instar larvae was conducted to investigate the hypothesis that geographic and temporal dietary differences are reflected in changes in organismal size.
Testing Viability Of Oligonucleotide Primers On Microsatellite Markers Amplified By Polymerase Chain Reaction On Dermacentor Variabilis Samples From The Bulloch County Region Of Georgia, Usa, Danielle Souza
Honors College Theses
Dermacentor variabilis, more commonly known as the wood tick, or the American dog tick, can be found all over North America. D. variabilis is a common tick in Bulloch county, Georgia, USA, and little is known about the population structure of this population of the species. Microsatellite markers are able to locate sections of the genome and can aid in estimating relatedness among individuals in a population. Six different oligonucleotide primer pairs were published for D. variabilis, however, published and non-published works indicated that not all of those primer pairs were working equally well for PCR. This study is …
Comparison Of Three Benthic Macroinvertebrate Passive Sampling Devices For Non-Wadeable Streams, Kelsey A. Laymon
Comparison Of Three Benthic Macroinvertebrate Passive Sampling Devices For Non-Wadeable Streams, Kelsey A. Laymon
College of Graduate Studies: Theses & Dissertations
Rivers and streams provide essential ecosystem services to the degree that the monitoring and maintenance of these systems becomes imperative. Biomonitoring provides managers and policymakers with the tools to make informed decisions, and macroinvertebrates are often the object of biomonitoring because they are ubiquitous in most systems and are known to be good indicators of water quality. However, methods for sampling macroinvertebrates in non-wadeable streams (i.e., large rivers) have not been standardized across states and regions and an established method for macroinvertebrate biomonitoring in large rivers of Georgia is not currently available. My study compared macroinvertebrates collected with three types …
Blow Fly (Diptera: Calliphoridae) Responses To Different Colors Of Baited Traps, Oluwadamilola Olufunso Oke
Blow Fly (Diptera: Calliphoridae) Responses To Different Colors Of Baited Traps, Oluwadamilola Olufunso Oke
Honors College Theses
Blow flies (Diptera: Calliphoridae) are usually the first insects to colonize human remains. By determining the time of colonization, a postmortem interval (PMI), or “time of death”, can be estimated. To develop more accurate PMI estimates, it is important for forensic entomologists to understand the cues that Blow flies use to locate vertebrate remains. The purpose of this study was to determine whether Blow flies use visual cues, in addition to olfactory cues, to locate carrion. Two colors of fly traps, clear and green, were constructed and chicken gizzard used as bait. Three Blow fly species exhibited a significant preference …
Biogeographical Patterns In The Hard-Tick Genus Amblyomma Koch 1844 (Acari: Ixodidae), Matthew H. Seabolt
Biogeographical Patterns In The Hard-Tick Genus Amblyomma Koch 1844 (Acari: Ixodidae), Matthew H. Seabolt
College of Graduate Studies: Theses & Dissertations
Amblyomma Koch is a genus of hard-ticks with approximately 130 species. Its geographical range is typical for organisms with a Gondwanan origin. A majority of these species are endemic to the Neo- and Afrotropical regions, with the remaining taxa dispersed throughout Southeast Asia, Australia and the Pacific islands. Based on this distribution, we hypothesize that the genus dispersal patterns will mirror the fragmentation and continental drift of the Gondwanan supercontinent. Maximum parsimony, maximum likelihood, Bayesian inference and node-dating analyses of nuclear 18S rDNA gene sequences reveal a more recent origin and radiation patterns within the genus and suggest that Amblyomma …
The Behavioral Response Of Culex Erraticus To Different Snake Odors, Lindsey E. Wells
The Behavioral Response Of Culex Erraticus To Different Snake Odors, Lindsey E. Wells
Honors College Theses
Eastern equine encephalitis virus (EEEV) is an arbovirus that can cause fatal infections in humans and horses. Unfortunately, the transmission mechanisms of this virus are still largely unknown. Culex erraticus displays a strong potential for serving as a vector of EEEV because of its indiscriminate feeding pattern and abundance in areas with the highest prevalence of infection. However, Culex erraticus is incapable of over-wintering the virus, yet EEE recurs each spring. Snakes may play an important role in over-wintering the virus, and certain snake species may be infected more frequently than others. This study was conducted to determine if Culex …