Ecology and Evolutionary Biology Commons^™

Developmental Mechanisms For The Diversification Of Polyphenic Morphs In The Head Horn Of Onthophagine Beetles (Coleoptera: Scarabaeidae Onthophagus Taurus): Plasticity Through Nutrition, Logan Paul Zeigler

Graduate Theses, Dissertations, and Problem Reports (ETD)

Developmental plasticity is the phenotypic variation between organisms that is caused by environmental interactions affecting the developmental systems of organisms. The research focused primarily on nutrition-responsive developmental plasticity. In this research we used the nutritionally determined head horn development of Onthophagus taurus to better understand the developmental mechanisms and genetic underpinnings of nutrition-responsive trait development. We focused specifically on altering the availability of specific nutrition-related primary metabolites, cholesterol and palmitic acid, identified in the activity of The Hedgehog pathway, a critical pathway in head horn development. By altering diet composition using cholesterol, reducing transcript expression of an acyltransferase gene, rasp …

Determination Of Iron-Reducing Bacterial Activities In Lake Sediments., Alexandra Kahn

Williams Honors College, Honors Research Projects

Algal blooms are growing rampantly in lacustrine systems due to an increase of phosphorus, a nutrient for algae. Phosphorus is being released into the environment causing overpopulation and eutrophication which damages the ecosystem. The availability of phosphorus is affected by the solubility of iron which is regulated through iron reducing and iron oxidizing bacteria. Phosphorus adheres to insoluble Fe(III), which prohibits algae from utilizing it, while phosphorus does not attach to soluble Fe(II) and therefore it remains available to algae. The purpose of this study was to determine how bacteria influence iron solubility and what are the ideal environmental conditions …

Are There Ecomorphologically-Related Differences In The Adhesive Setae Of Jamaican Anolis Lizards?, Caitlin Wright

Williams Honors College, Honors Research Projects

Anolis lizards are best known for their ability to adhere to surfaces with the use of their adhesive toe pads. Different ecomorphs of anoles exist which co-vary with habitat type, a concept known as ecomorphology. The ecomorphology of anoles has been well studied, however little is known about the differences that may exist within the adhesive toe pads of different anole species. In this project, differences within adhesive microstructures of five different species of Jamaican anoles was examined. Scanning electron microscopy (SEM) will be used to produce images of the adhesive structures in which a variety of morphological measurements were …

Bat Boxes As Mitigation Tools: Factors Impacting Microclimate And Myotis Sodalis Roost Selection, Reed Crawford

Online Theses and Dissertations

Environmental degradation has led to declines in available natural roosting habitat for bats. To mitigate this loss, practitioners often deploy artificial roosts (e.g., bat boxes). There are no established species-specific practices for deployment strategy and roost design selection, but occupancy rates are known to vary across species and roost microclimates can be harmful to bats. Providing bats with thermally beneficial roosts during summer could enhance overwinter survival of WNS-affected species. To further our understanding of roost preference and microclimate, we deployed 40 rocket box roosts of 5 designs at field sites in Indiana and Kentucky. Roosts were deployed in clusters …

The Importance Of Surface Mining As A Landscape Variable In Shaping Wild Bee Communities In Central Appalachian Forests, Amanda Sheryl Dunaway

Online Theses and Dissertations

Anthropogenic disturbance has led to widespread losses in biodiversity. Native bees (Hymenoptera, Apoidea) provide essential pollination services and have been declining in abundance dramatically in recent years. One potential cause of these declines is anthropogenic disturbance. Eleven sites were established in Southeastern Kentucky, U.S.A. where surface mining for coal is common. Data was analyzed using generalized linear mixed-effect models to detect the most important landscape scale variables, including mining, in shaping bee communities. Bee species richness was significantly lower on sites that contained surface mines, but abundance was similar between mined and unmined sites. The proportion of each nesting group …

Assessment Of Indiana Bat Reproductive Condition, Recapture Trends, And Temperatures Of Artificial Roosts In Kentucky, Michaela L. Rogers

Online Theses and Dissertations

Recovery of bat species impacted by white-nose syndrome (WNS) will necessarily require population growth. I assessed reproductive capability of the endangered Indiana bat (Myotis sodalis) in Kentucky, where WNS was first detected in hibernacula in April 2011. Due to loss of fat reserves associated with WNS infection, coupled with the energetic expenditures associated with pregnancy, I hypothesized mass of reproductively-active females captured during the maternity season would decrease across my data collection period (2017-2019). Further, I predicted that reproductive rates in the study population would be lower than historic rates for Kentucky. Mist net surveys around artificial maternity roosts at …

Abundance Of Songbirds In Eastern Hemlock Stands Following Chemical Treatment For Hemlock Woolly Adelgid, Natalie Sweeting

Online Theses and Dissertations

In the 1960’s the invasive Hemlock Woolly Adelgid (Adelges tsugae [hereafter, HWA]) began to spread west across the hemlock stands of the Eastern U.S. killing a significant number of Eastern Hemlock (Tsuga canadensis). While chemical treatments, primarily with the active ingredient imidacloprid, have been implemented, their effects on hemlock dependent avian species are largely unknown. A 2009 study, which took place as HWA was beginning to invade Kentucky, identified six indicator species that were positively and negatively correlated with eastern hemlock stands throughout the Appalachian Mountain region of Kentucky. Our study repeated bird and vegetation surveys at the same 65 …

Bee Abundance Along A Tropical Montane Elevational Gradient And Implications For Crop Pollination Services, Kristin M. Conrad

Online Theses and Dissertations

Tropical forests are among the biologically richest ecosystems on Earth, but how most organisms in these forests will respond to a warming climate remains uncertain. Insects are expected to be highly responsive to climate change due to their short life cycles that are strongly influenced by temperature. Plants depend on pollinators to set seed and reproduce, and many animal populations rely on the resources provided by flowering plants. There is an urgent need to document elevational distributions and thermal specialization for tropical bee species to understand how these important pollinators may respond to warming temperatures. My four-year study (2016-2019) aims …

Impacts Of Driving Factors On Annual Cicada Emergence In Relation To The Seasonal Abundance Of Eastern Copperheads (Agkistrodon Contortrix), Jesse Christian Sockman

Online Theses and Dissertations

For many species, life history events are cyclical and often correspond to specific environmental conditions. As a result of environmental variability, the optimal conditions that regulate the range and activity of highly regulated cyclical organisms, like cicadas, are subject to temporal change. Variability in cicada emergence and abundance has been shown to impact the species that rely on the rapid influx of nutrients. One such predator is the Eastern Copperhead (Agkistrodon contortrix) that has been noted feeding on emerging cicadas throughout their range. In eastern Kentucky, observations of copperheads congregating to feed on cicadas has been documented in recreational areas. …

The Effects Of Internal Physiology On Polyphenic Horn Development In The Dung Beetle Onthophagus Taurus, Naomi Garrett Williamson

Graduate Theses, Dissertations, and Problem Reports (ETD)

An organism’s phenotype can be affected in development by alterations to gene expression based on environmental inputs. Nutrition is one such environmental input and the central regulator of development of large horn or small horn phenotypes in the dung beetle species, Onthophagus taurus. However, little is known about the nature of chemical compounds that are critical to this plastic horn development. To better understand these compounds, we are utilizing an untargeted metabolomic approach as well as a targeted gene approach. Through the metabolomic approach, it was uncovered that environmental conditions tend to have a greater impact on metabolomic composition …

Leaf Choice By Salmonfly Nymphs (Pteronarcys Californica) In Western Montana, Emily L. Hamant, Art Woods, James Frakes

Undergraduate Theses, Professional Papers, and Capstone Artifacts

No abstract provided.

The Evolution Of Dragons, Laura J. Mayfield

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Dragons have been depicted in human art as early as 4500 BCE. For centuries, these fantasy creatures have inspired countless folk and fantasy tales, as well as appearing in the art of different cultures around the world. Now there are thousands of different depictions of these huge, flying, fire-breathing lizards, but are any of them possible? In this study, I reference peer-reviewed scientific articles, phylogenetic analysis, and paleoart studies to create biologically-sound dragons. Basing the dragon lineage on a real branch of webbed-winged scansoriopterygids—an extinct family of climbing and gliding maniraptoran dinosaurs—I explored the possible wing-structure, fire-breathing abilities, and effects …

The Effects Of Light Level And Temperature On Freshwater Microbial Biomass, Production, Enzyme Activity, And Community Composition, Joel Bonney

Master's Theses and Doctoral Dissertations

Algae can stimulate the production of heterotrophic microbes in periphyton communities, but the synergistic effects of light and temperature on this phenomenon are not well understood and have implications on carbon cycling and energy flow. We employed recirculating mesocosms held at different temperatures and light levels and filled with Typha litter and attached microbes to assess these effects. Heterotrophic microbial production was greater in the presence of light, as were some periphytic enzyme activities. Few response variables increased in a near-linear fashion with ascending temperature, suggesting different temperature optima. The strength of heterotrophic microbial production responses to algal photosynthesis did …

Leaf Angle And Leaf Stomata Responses To Experimental Drought In Quercus Velutina And Acer Saccharum, Brittany Nichole Casey

Graduate Theses, Dissertations, and Problem Reports (ETD)

The possibility of increased severity and frequency of drought conditions, as a result of global climate variability, greatly complicates our ability to forecast future forest functions such as productivity and carbon sequestration. Assessing how tree species vary in their response to drought can aid in predicting the impact on forest ecosystems as a whole. Throughfall exclusion (TfE) experiments are potentially useful tools to simulate realistic drought conditions within intact forest ecosystems. We employed a TfE experiment during the 2018 growing season within the WV Land Trust’s Elizabeth’s Woods Nature Preserve, near Morgantown, WV, to assess the leaf angle and leaf …

Tree Growth And Water Use Efficiency During The Twentieth Century: From Global Trends To Local Drivers, Justin Michael Mathias

Graduate Theses, Dissertations, and Problem Reports (ETD)

The frequency and severity of extreme environmental conditions will continue to increase under global environmental change. How terrestrial plants respond to prolonged, and often novel environmental stressors, will have profound impacts on, and feedbacks with, the Earth climate system at local to continental scales. Central to these feedbacks are plant stomata, actively regulated pores on the leaves of plants that act as a control valve over the fluxes of carbon dioxide (CO₂) into the leaf during photosynthesis and water vapor (H₂O) out of the leaf during transpiration. Importantly, changes in stomatal aperture do not affect the …

Avian And Salamander Response To Young Forest Management In West Virginia, Eric L. Margenau

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation focuses on the effects of various young forest habitat management techniques on the avian and salamander community in West Virginia. Wildlife species associated with the nascent stage of forest succession are experiencing precipitous population declines throughout much of the eastern United States due to decreases in the amount of young forest area which have been brought on by changes in disturbance regimes over the past century. As a result, the need to find novel approaches for creating young forest habitats to sustain young forest wildlife populations is necessary. However, young forest habitat creation often negatively affects species that …

Nitrogen Fertilization Decouples Roots And Microbes In Temperate Forests: Impacts On Soil Carbon And Nitrogen Cycling, Joseph E. Carrara

Graduate Theses, Dissertations, and Problem Reports (ETD)

Since the start of the industrial revolution the burning of fossil fuels has resulted in enhanced nitrogen (N) inputs into temperate forests through atmospheric deposition. As N is the limiting nutrient for tree growth across most forests, these inputs have generally enhanced above-ground biomass accumulation. However, the impacts of added N on soil carbon storage (C) are less straightforward. While the mean N response across studies is an enhancement of soil C, these results are variable with some studies reporting net C losses. The classic paradigm posits that N enhances soil C through negative effects on fungal decomposers. However, some …

From Organisms To Ecosystems: Impacts Of Limb Loss And Regeneration On Crayfish Behavior, Luc Arnaud Dunoyer

Theses and Dissertations--Biology

The ability of some organisms to regenerate tissues and organs has fascinated naturalists since antiquity, dating back to the earliest accounts of scientific inquiry with Aristotle in Ancient Greece. Then, Darwin’s theory invigorated some scientists’ dream of stimulating (or reactivating) regenerative capacities in human beings by showing that we are related to highly regenerative organisms. More recently, a renewed interest in discovering the molecular and genetic basis for organ and tissue regeneration has led biologists to focus more specifically on a restrictive set of model organisms.

Although the process of limb regeneration is different between invertebrate and vertebrate organisms, it …

From Genes To Species: Ecological Speciation With Gene Flow In Neodiprion Pinetum And N. Lecontei, Emily E. Bendall

Theses and Dissertations--Biology

My dissertation focuses on how differences accumulate across the genome during ecological speciation with geneflow. To do this I used two species of Neodiprionpine sawflies, which are plant-feeding hymenopterans with high host specificity. I used experimental crosses to measure both intrinsic and extrinsic postzygotic isolation and to understand the contribution of specific traits to reproductive isolation. Despite substantial genetic divergence and haploid males in which all recessive incompatibilities should be expressed, I found surprisingly little evidence of intrinsic postzygotic isolation. Recombination in hybrid males may reconstitute viable genotypes and counteract the effects of haploidy in males. Nevertheless, hybrids have …

Assessing The Hierarchy Of Long-Term Environmental Controls On Diatom Communities Of Yellowstone National Park Using Lacustrine Sediment Records, Victoria Chraibi, Sherilyn C. Fritz

Department of Earth and Atmospheric Sciences: Faculty Publications

An ecosystem’s ability to maintain structure and function following disturbance, defined as resilience, is influenced by a hierarchy of environmental controls, including climate, surface cover, and ecological relationships that shape biological community composition and productivity. This study examined lacustrine sediment records of naturally fishless lakes in Yellowstone National Park to reconstruct the response of aquatic communities to climate and trophic cascades from fish stocking. Sediment records of diatom algae did not exhibit a distinct response to fish stocking in terms of assemblage or algal productivity. Instead, 3 of 4 lakes underwent a shift to dominance by benthic diatom species from …

Energy Development And Production In The Great Plains: Implications And Mitigation Opportunities, Jacqueline P. Ott, Bruce B. Hanberry, Mona Khalil, Mark W. Paschke, Max Post Van Der Burg, A.J. Prenni

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Energy is an integral part of society. The major US energy sources of fossil fuels (coal, oil, natural gas); biofuels (ethanol); and wind are concentrated in grassland ecosystems of the Great Plains. As energy de- mand continues to increase, mounting pressures will be placed on North American grassland systems. In this review, we present the ecological effects of energy development and production on grassland sys- tems. We then identify opportunities to mitigate these effects during the planning, construction, and pro- duction phases by using informed methodology and improved technology. Primary effects during energy development include small- and large-scale soil disturbance …

Vertical‑Surface Navigation In The Neotropical Whip Spider Paraphrynus Laevifrons (Arachnida: Amblypygi), Patrick Casto, Daniel D. Wiegmann, Vincent J. Coppola, Daniele Nardi, Eileen A. Hebets, Verner P. Bingman

Eileen Hebets Publications

Studies on whip spider navigation have focused on their ability to locate goal locations in the horizontal plane (e.g., when moving along the ground). However, many species of tropical whip spiders reside and move along surfaces in the vertical plane (e.g., trees). Under controlled laboratory conditions, the current study investigated the ability of the tropical whip spider, Paraphrynus laevifrons, to return to a home shelter on a vertical surface in the presence of numerous, similar, and competing refuge sites, as well as the distribution of navigational errors in the vertical, horizontal, and diagonal plane. We also assessed the relative …

Trade-Offs Between Morphology And Thermal Niches Mediate Adaptation In Response To Competing Selective Pressures, Stella F. Uiterwaal, Ian T. Lagerstrom, Thomas M. Luhring, Miranda E. Salsbery, John P. Delong

School of Biological Sciences: Faculty Publications

Abstract

The effects of climate change—such as increased temperature variability and novel predators—rarely happen in isolation, but it is unclear how organisms cope with multiple stressors simultaneously. To explore this, we grew replicate Paramecium caudatum populations in either constant or variable temperatures and exposed half to predation. We then fit thermal performance curves (TPCs) of intrinsic growth rate (r_max) for each replicate population (N = 12) across seven temperatures (10°C–38°C). TPCs of P. caudatum exposed to both temperature variability and predation responded only to one or the other (but not both), resulting in unpredictable outcomes. These changes in …

Multi-Isotopic (Δ2h, Δ13c, Δ15n) Tracing Of Molt Origin For European Starlings Associated With U.S. Dairies And Feedlots, Scott Werner, J. W. Fischer, Keith A. Hobson

United States Department of Agriculture Wildlife Services: Staff Publications

Introduced bird species can become invasive in agroecosystems and their management is inhibited if their origin and movements are not well understood. Stable isotope measurements of feathers can be used to infer molt origins and interstate movements in North America. We analyzed stable-hydrogen (δ²H), carbon (δ¹³C) and nitrogen (δ ¹⁵N) isotope ratios in feathers to better understand the molt origin of European starlings (Sturnus vulgaris) collected at dairies and feedlots throughout the United States. Primary feathers were used from 596 adult and 90 juvenile starlings collected during winter at dairies …

Tissue Tropisms Of Avian Influenza A Viruses Affect Their Spillovers From Wild Birds To Pigs, Xiaojian Zhang, Fred L. Cunningham, Lei Li, Katie Hanson-Dorr, Liyuan Liu, Kaitlyn Waters, Minhui Guan, Alicia K. Olivier, Brandon S. Schmit, Jacqueline M. Nolting, Andrew S. Bowman, Mia Kim Torchetti, Thomas J. Deliberto, Xiu-Feng Wan

United States Department of Agriculture Wildlife Services: Staff Publications

Wild aquatic birds maintain a large, genetically diverse pool of influenza A viruses (IAVs), which can be transmitted to lower mammals and, ultimately, humans. Through phenotypic analyses of viral replication efficiency, only a small set of avian IAVs were found to replicate well in epithelial cells of the swine upper respiratory tract, and these viruses were shown to infect and cause virus shedding in pigs. Such a phenotypic trait of the viral replication efficiency appears to emerge randomly and is distributed among IAVs across multiple avian species and geographic and temporal orders. It is not determined by receptor binding preference …

Repellent Surface Applications For Pest Birds, Shelagh T. Deliberto, James C. Carlson, Hailey E. Mclean, Caroline S. Olson, Scott J. Werner

United States Department of Agriculture Wildlife Services: Staff Publications

Common pest birds in the United States include the non-native European starling (Sturnus vulgaris), house sparrow (Passer domesticus), and the pigeon (Columba livia domestica), as well as native birds including Canada geese (Branta canadensis) and gull species (Laridae). Large concentrations of pest birds can create human health hazards and monetary losses due to consumption of crops, depredation, and fecal contamination and accumulation. Fecal contamination hazards include the potential spread of zoonotic diseases including antimicrobial-resistant zoonoses and human injury due to the accumulation of fecal material on walking surfaces. Additionally, fecal accumulation causes …

Extreme Offspring Ornamentation In American Coots Is Favored By Selection Within Families, Not Benefits To Conspecific Brood Parasites, Bruce E. Lyon, Daizaburo Shizuka

School of Biological Sciences: Faculty Publications

Offspring ornamentation typically occurs in taxa with parental care, suggesting that selection arising from social interactions between parents and offspring may underlie signal evolution. American coot babies are among the most ornamented offspring found in nature, sporting vividly orange-red natal plumage, a bright red beak, and other red parts around the face and pate. Previous plumage manipulation experiments showed that ornamented plumage is favored by strong parental choice for chicks with more extreme ornamentation but left unresolved the question as to why parents show the preference. Here we explore natural patterns of variation in coot chick plumage color, both within …

Coproid Predicts The Source Of Coprolites And Paleofeces Using Microbiome Composition And Host Dna Content, Maxime Borry, Bryan Cordova, Angela Perri, Marsha Wibowo, Tanvi Prasad Honap, Jada Ko, Kate Britton, Linus Girdland-Flink, Robert C. Power, Ingelise Stuijts, Domingo C. Salazar-García, Courtney Hofman, Richard Hagan, Thérèse Samdapawindé Kagoné, Nicolas Meda, Helene Carabin, David Jacobson, Karl Reinhard, Cecil Lewis, Aleksandar Kostic, Choongwon Jeong, Alexander Herbig, Alexander Hübner, Christina Warinner

Karl Reinhard Publications

Shotgun metagenomics applied to archaeological feces (paleofeces) can bring new insights into the composition and functions of human and animal gut microbiota from the past. However, paleofeces often undergo physical distortions in archaeological sediments, making their source species difficult to identify on the basis of fecal morphology or microscopic features alone. Here we present a reproducible and scalable pipeline using both host and microbial DNA to infer the host source of fecal material. We apply this pipeline to newly sequenced archaeological specimens and show that we are able to distinguish morphologically similar human and canine paleofeces, as well as non-fecal …

Diet Analysis Reveals Pre-Historic Meals Among The Loma San Gabriel At La Cueva De Los Muertos Chiquitos, Rio Zape, Mexico (600–800 Ce), Elisa Pucu, Julia Russ, Karl Reinhard

Karl Reinhard Publications

Coprolites have been a source of study for archeologists due to several reasons: they not only provide information on the life and nutritional habits of ancient individuals but also on their health. In this paper, we processed 10 coprolites collected at La Cueva de Los Muertos Chiquitos (600–800 CE), Rio Zape, Mexico, with acetolysis solution for pollen analysis. The number of pollen grains/gram of each coprolite sample was quantified along with the macroscopic remains of these samples. The main food item ingested by the population was maize, followed by Agave. Squash blossoms were also part of their food source …

The Natural History Of The Marsh Rice Rat, Oryzomys Palustris, In Eastern Virginia, Robert K. Rose

Biological Sciences Faculty Publications

The marsh rice rat, Oryzomys palustris, is a common rodent in tidal marshes of eastern Virginia, including those on the barrier islands. It also is present in grassy old fields in upland habitats in the coastal plain and parts of the piedmont of Virginia. This report summarizes what has been learned in recent decades about the population biology of this species in Virginia, including aspects of behavior, density, diet, distribution, genetics, habitats, mammal associates, and reproduction.