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Articles 1381 - 1410 of 36475
Full-Text Articles in Animal Sciences
Assessing Reproductive Health In Zoo-Managed African Elephant Bulls (Loxodonta Africana), Kaitlyn Marie Campbell
Assessing Reproductive Health In Zoo-Managed African Elephant Bulls (Loxodonta Africana), Kaitlyn Marie Campbell
Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–
Populations of zoo-managed African elephant (Loxodonta Africana) exhibit substantial individual- and population-level variation in reproductive success, yet research has largely focused on females, leaving male fertility poorly understood. Subfertility in bulls, characterized by low libido and inconsistent semen quality, poses significant challenges to genetic management and long-term population sustainability. This study a) systematically evaluates semen quality, b) identifies physiological, behavioral, social, and environmental determinants of fertility, and c) advances ex situ conservation through the expansion of the African elephant sperm bank. Between January 2023 and July 2025, 209 semen collection events were conducted from 13 sexually mature bulls …
From Sequencing To Conservation: Genomic Analysis Of Three Sportfish Species In West Virginia, Andrew Johnson
From Sequencing To Conservation: Genomic Analysis Of Three Sportfish Species In West Virginia, Andrew Johnson
Graduate Theses, Dissertations, and Problem Reports (ETD)
Situated in the Appalachian Mountains, one of the oldest mountain ranges on Earth, West Virginia waters boast rich ichthyofauna including native sportfish species walleye (Sander vitreus), largemouth bass (Micropterus nigricans), and muskellunge (Esox masquinongy). These three species are all native to the contemporary Ohio River watershed and play a major recreational and ecological role in local fisheries. Ecologically, all three species are apex predators and play a key role in ecosystems by directly influencing local fish assemblages. As dominant apex predators all three species are highly sought after in recreational fisheries with largemouth bass …
Exploring The Ruminal Microbiome And Its Impacts On Feed Efficiency In Beef Cattle, Taylor Simone Sidney
Exploring The Ruminal Microbiome And Its Impacts On Feed Efficiency In Beef Cattle, Taylor Simone Sidney
Graduate Theses, Dissertations, and Problem Reports (ETD)
The ability for beef cattle to utilize feed efficiently is a critical determinant for both economic and environmental sustainability in livestock production. However, despite extensive research, the complex microbial ecosystem within the rumen and its relationship to feed efficiency remain incompletely understood. This dissertation explores the physiological and microbial foundations of feed efficiency by characterizing how rumen microbial communities and their functions differ among beef cattle with divergent residual feed intake (RFI) phenotypes and how the RFI status of beef cattle influenced their response to dietary interventions such as supplementation with a Bacillus-based feed additive. In the first study, …
Effects Of Environmental And Anthropogenic Gradients On Fish Beta Diversity In Central Appalachian Streams, Alanna Kaylee Lowther Smith
Effects Of Environmental And Anthropogenic Gradients On Fish Beta Diversity In Central Appalachian Streams, Alanna Kaylee Lowther Smith
Graduate Theses, Dissertations, and Problem Reports (ETD)
Understanding the environmental and spatial drivers of community composition is essential for conserving freshwater biodiversity. We examined how environmental gradients, land cover, and spatial structure influence fish beta diversity in wadeable streams across West Virginia, USA. We quantified total beta diversity and partitioned it into species replacement and richness difference components and used local contributions to beta diversity (LCBD) to identify compositionally unique sites. Community–environment relationships were evaluated with distance-based redundancy analysis (dbRDA), and a beta regression GLMM was used to test how elevation, slope, land cover, and stream order (factor) predicted LCBD while accounting for spatial structure (dbMEMs) and …
Effects Of Variable Nitrogen Fertilization Rates And Dried Distillers Grains Plus Solubles Supplementation On Forage Use, Animal Performance, And Economic Outcomes Of Steer Calves Grazing Winter Wheat Pastures, Cody E. Gruber, Miriam A. Snider, Michelle L. Johnson, Tom Hess, Elizabeth B. Kegley, J. Daniel Rivera, James A. Mitchell, M. Shane Gadberry
Effects Of Variable Nitrogen Fertilization Rates And Dried Distillers Grains Plus Solubles Supplementation On Forage Use, Animal Performance, And Economic Outcomes Of Steer Calves Grazing Winter Wheat Pastures, Cody E. Gruber, Miriam A. Snider, Michelle L. Johnson, Tom Hess, Elizabeth B. Kegley, J. Daniel Rivera, James A. Mitchell, M. Shane Gadberry
Animal Science Faculty Publications and Presentations
With the continued increase of fertilizer prices, stocker cattle producers may utilize alternative management strategies to mitigate costs and extend the grazing season to achieve better utilization of a wheat (Triticum aestivum L.) crop. One potential strategy is to reduce pasture nitrogen (N) fertilization rates combined with animal dietary supplementation. In this 3-year (yr) study, wheat pastures (1.62-hectare (ha)) were stocked at fixed rates of 2.47 and 4.94 steer/ha in the fall and spring respectively. Nitrogen was applied as urea to experimental pastures at three different rates: 1) 100.9 (100N), 2) 134.5 (135N), and 3) 168.1 kg N/ha (168N). These …
Evaluating And Mapping Habitat Suitability And Effects Of Woodland Treatment On Breeding Pinyon Jays In Colorado, Emily Diane Macklin
Evaluating And Mapping Habitat Suitability And Effects Of Woodland Treatment On Breeding Pinyon Jays In Colorado, Emily Diane Macklin
Electronic Theses and Dissertations
The pinyon jay (Gymnorhinus cyanocephalus) is a highly social, colonially nesting corvid endemic to the interior western United States whose populations have declined by ~72% since 1966, prompting the species’ current review for listing under the Endangered Species Act. Pinyon jays inhabit pinyon pine (Pinus subsect. Cembroides) and juniper (Juniperus spp.) woodlands and other dry conifer woodlands, portions of which are treated through tree thinning and removal for fuels reduction, ecological restoration, and habitat improvement for wildlife. As a result there is pressing need to better understand how woodland treatment and other potential drivers impact pinyon jay habitat suitability and …
Lives And Lessons Of Zoo Animals, Nicole Emlen
Lives And Lessons Of Zoo Animals, Nicole Emlen
Undergraduate Theses, Professional Papers, and Capstone Artifacts
This project was an interdisciplinary museum exhibit that was displayed at the Montana Natural History Center between April 4 and May 12, 2025. It displays my photographs that I took of some of the animals I worked with during my summer 2024 internship at ZooMontana. In this project, I educate the public about three main functions of zoos--rescue, conservation, and education--through the stories and lives of four species. These animals all have unique stories that are important to share to improve public knowledge of why zoos are important and how we can improve relationships between humans and wildlife. Photography is …
Assessing Intra-Seasonal Changes In Summer Elk Diet Composition, Ava E. Window
Assessing Intra-Seasonal Changes In Summer Elk Diet Composition, Ava E. Window
Undergraduate Theses, Professional Papers, and Capstone Artifacts
Elk (Cervus canadensis) require adequate forage to fulfill their seasonal nutritional requirements. Elk undergo their highest nutritional demands during summer to support critical life functions such as late gestation, lactation, and juvenile growth. However, variations in plant phenology significantly influence the quality and quantity of forage available to elk during the summer season. As summer advances and plant communities mature, forage quality often declines, leading to a landscape that imposes greater nutritional constraints on elk. As a result, prior research on elk foraging habits has primarily focused on late summer. This has led to limited knowledge surrounding the …
Effect Of Vibration Isolation During Transportation On Beef Sirloin Subprimal And Steak Quality, Kylie Nevada Krueger
Effect Of Vibration Isolation During Transportation On Beef Sirloin Subprimal And Steak Quality, Kylie Nevada Krueger
Electronic Theses and Dissertations
The objective of this study was to determine the influence of vibration during transportation on beef sirloin water holding capacity, color, and tenderness. USDA Choice center-cut sirloin top butts (n = 45 cases) were collected at a commercial processing facility and randomly allocated to one of three treatments 1) regular pallet driven 241 km (Reg241), 2) regular pallet driven 644 km (Reg644), and 3) vibration isolation pallet driven 644 km (Iso644). All subprimals were transported at -2.2 °C (d0) and stored for 10 days to mimic industry practices for product aging. Following storage (d11), one subprimal per case was evaluated …
Development Of A Proposed Selective Medium For The Isolation And Differentiation Of P. Sordellii, Dimyana Bottros
Development Of A Proposed Selective Medium For The Isolation And Differentiation Of P. Sordellii, Dimyana Bottros
Honors Undergraduate Theses
Clostridium, a genus of anaerobic, spore-forming bacteria, includes clinically and ecologically significant species like Clostridium difficile and Paeniclostridium sordellii. C. difficile causes antibiotic-associated colitis, while P. sordellii is linked to fatal infections and spontaneous abortions. Recent studies found both species in marine sediments, suggesting their role in environmental and wildlife health disturbances, including the 2013 mass mortality of over 300 manatees in the Indian River Lagoon. However, isolating and differentiating P. sordellii is challenging due to the lack of selective media optimized for its growth. This thesis proposes a novel selective medium tailored to P. sordellii’s physiological …
Utilization Of Golden Pennycress In Broiler Diets, Marley M. Knowles
Utilization Of Golden Pennycress In Broiler Diets, Marley M. Knowles
Theses and Dissertations
The objective of this study was to evaluate performance in broiler chickens fed diets containing golden pennycress meal (GPCM), with and without a flavor additive. Golden pennycress (Thlaspi arvense) is a winter cover crop grown in the Midwest that is rising in popularity as a cash crop used for biofuel production. After oil is pressed out of the seeds, a meal remains that is usually discarded. Incorporation of this meal (23% protein, 15% fat) into feedstuffs for broilers would be an efficient way to make biofuel production more sustainable. In one study, Cornish Cross three-day-old chicks (n=12) were …
Fluke Egg Sedimentation Test Procedure, Department Of Primary Industries And Regional Development, Western Australia
Fluke Egg Sedimentation Test Procedure, Department Of Primary Industries And Regional Development, Western Australia
Animal production and livestock factsheets
The department’s approved procedure for detecting trematode eggs and the Eimeria leuckarti sedimentation method (FEST) on faecal samples.
Investigating Relatedness Within Breeding Units Of The Highly Social Bushtit (Psaltriparus Minimus), Fiona Skye Connelly
Investigating Relatedness Within Breeding Units Of The Highly Social Bushtit (Psaltriparus Minimus), Fiona Skye Connelly
Theses and Dissertations--Biology
Cooperative breeding systems represent an array of varied reproductive strategies and remain a topic of interest in behavioral ecology (i.e., Riehl 2013, Cornwallis et al. 2017). Different manifestations of cooperative breeding, such as cooperative polyandry, no doubt have unique evolutionary pressures tied to local ecologies which have led to their proliferation. Of particular interest are species that show flexibility in their use of reproductive strategies, wherein individuals may choose one strategy over another given a specific circumstance. Here, I used double-digest restriction site-associated DNA sequencing (i.e., ddRAD-seq) to investigate bushtit (Psaltriparus minimus) reproductive strategies and the relatedness between …
Exploring Mating Patterns: The Interplay Of Genes, Environment, And Their Evolutionary Outcomes, Sanduni Talagala
Exploring Mating Patterns: The Interplay Of Genes, Environment, And Their Evolutionary Outcomes, Sanduni Talagala
Theses and Dissertations (Comprehensive)
There is great variation in the reproductive tactics exhibited both between and within different species, which is reflected in how individuals engage in mate assessment, mate choice and offspring production. It is therefore important to understand general mating patterns of populations, the factors that influence these mating patterns, and the consequences they carry, in order to get a more holistic understanding of mating systems. This doctoral research represents a multi-pronged investigation of the factors shaping mating patterns and their consequences, how environmental factors influence mating patterns, and how both genotypes and the environment interact to influence mate choice decisions and …
Validating A Novel Capability Of Assessing Pathways Of Animal Water Gain And Loss, Zachary T. Steele, Karen Caceres, Zachary A. David, Lisa M. Shollenberger, Alexander R. Gerson, Seth D. Newsome, John P. Whiteman
Validating A Novel Capability Of Assessing Pathways Of Animal Water Gain And Loss, Zachary T. Steele, Karen Caceres, Zachary A. David, Lisa M. Shollenberger, Alexander R. Gerson, Seth D. Newsome, John P. Whiteman
Biological Sciences Faculty Publications
Understanding variations in the routes by which wild animals gain and lose water is challenging, and common methods require longitudinal sampling, which can be prohibitive. However, a new approach uses Δ′¹⁷OBW (Δ′¹⁷O of animal body water), calculated from measurements of δ′¹⁷O and δ′¹⁸O in a single sample, as a natural tracer of water flux. Δ′¹⁷OBW is promising, but its relationship to organismal variables such as metabolic rate and water intake have not been validated. Here, we continuously measured oxygen influxes and effluxes of captive deer mice (Peromyscus maniculatus), and manipulated their water intake and metabolic rate. We used …
Addressing The Productivity-Susceptibility Analysis In Data-Limited Fisheries For Understudied Fish In The Marine Aquarium Trade, Alice Wynn, Jemelyn Baldisimo, Gabrielle Baillargeon, Michael Tlusty, Andrew Rhyne
Addressing The Productivity-Susceptibility Analysis In Data-Limited Fisheries For Understudied Fish In The Marine Aquarium Trade, Alice Wynn, Jemelyn Baldisimo, Gabrielle Baillargeon, Michael Tlusty, Andrew Rhyne
Biological Sciences Faculty Publications
The marine aquarium trade (MAT) harvests millions of coral reef fishes from the wild yearly. While the MAT supports fishers, hobbyists and marine education, harvested fishes are crucial in maintaining coral reef health and balanced marine cycles within their native habitats. Understanding the impact of the MAT on fish populations is critical for sustaining the trade in the long-term. Productivity-susceptibility analyses (PSAs) offer semiquantitative methods of analyzing the productive capacity of each fish stock, as well as its susceptibility to overfishing. The present study revises and expands upon a PSA conducted by Baillargeon et al. (2020) that focused on 32 …
A Multispecies, Multisite Assessment Of Coral Thermal Tolerances In American Samoa, Using Acute Heat Stress Assays, Ponchanok Weeriyanun, Nicolas R. Evensen, Katherine E. Parker, Veronica Z. Radice, Daniel J. Barshis
A Multispecies, Multisite Assessment Of Coral Thermal Tolerances In American Samoa, Using Acute Heat Stress Assays, Ponchanok Weeriyanun, Nicolas R. Evensen, Katherine E. Parker, Veronica Z. Radice, Daniel J. Barshis
Biological Sciences Faculty Publications
Reef-building corals are being impacted by increasing seawater temperatures, with marine heatwaves leading to mass bleaching events and extensive mortality. However, corals can display variation in thermal tolerance, both within and among species. In this study, we sampled four coral species (Acropora hyacinthus, Montipora grisea, Pocillopora verrucosa, and Porites lobata) from two sites on Tutuila, American Samoa, with contrasting environmental regimes but similar Maximum Monthly Means (MMM). Coral fragments were subjected to short-term acute heat stress experiments using the Coral Bleaching Automated Stress System (CBASS). PAM Fluorometry was used to measure the symbiont’s effective quantum yield (Fv/Fm) following heat stress …
Herpetofauna Richness, Diversity, And Occurrence At The Northern Ecotone Of Longleaf Pine, Julianne Jones, Dylan Bryant, Erik Yando
Herpetofauna Richness, Diversity, And Occurrence At The Northern Ecotone Of Longleaf Pine, Julianne Jones, Dylan Bryant, Erik Yando
Biological Sciences Faculty Publications
Habitat suitability commonly differs between the core and periphery of ecosystems. The longleaf pine ecosystem is recognized for its value in providing habitat to a variety of specialist and endemic species, especially herpetofauna. However, at the northernmost extent of longleaf pine (in southeastern Virginia) little is known about the denizen reptiles and amphibians. Moreover, the remaining longleaf pine ecosystem at the latitudinal ecotone is fragmented amongst a mosaic of other ecosystems. We examined herpetofauna diversity, richness, and capture rates between four focal habitat types—mature longleaf pine, restored longleaf pine, maple-gum swamp, and pocosin-bog—in the Zuni Pine Barrens of southeastern Virginia. …
Low Salinities Impair Heat Tolerance And Alter Reproductive Traits With No Implications For Survival In A Marine Annelid From Coastal Habitats, Gabrielle Newton, Gloria Massamba-N'Siala
Low Salinities Impair Heat Tolerance And Alter Reproductive Traits With No Implications For Survival In A Marine Annelid From Coastal Habitats, Gabrielle Newton, Gloria Massamba-N'Siala
Biological Sciences Faculty Publications
Climate change is expected to intensify salinity and temperature fluctuations in estuarine and coastal habitats. However, the impact of salinity variation on ectotherms’ thermal tolerance remains underexplored, limiting our understanding of the interplay between these stressors. This study investigated salinity-dependent changes in life history traits (survival, growth rate, fecundity, and egg size) over a 33-day exposure to a salinity gradient (12, 17, 22, 27, 32, 35, 40, 45, 50) in the marine annelid Ophryotrocha labronica. Individuals that survived the salinity treatments were assessed for their upper and lower thermal tolerance limits. Female individuals, the target of the study, exhibited …
Hair Growth Rate Estimation In North American Ursids, Jennifer H. Stern, Karyn D. Rode, Craig A. Stricker, Sheriden Ploof, Cindy L. J. Roberts, Celess Edinger, Mary Humbyrd, Nate Wagner, Megan A. Owen, John P. Whiteman, Thea Bechshoft, Brent White, Kristin L. Laidre
Hair Growth Rate Estimation In North American Ursids, Jennifer H. Stern, Karyn D. Rode, Craig A. Stricker, Sheriden Ploof, Cindy L. J. Roberts, Celess Edinger, Mary Humbyrd, Nate Wagner, Megan A. Owen, John P. Whiteman, Thea Bechshoft, Brent White, Kristin L. Laidre
Biological Sciences Faculty Publications
The feeding ecology of wildlife populations has important implications for individual health, population productivity and distribution patterns. For ursids (bears), food resources and feeding behaviour primarily affect population dynamics via effects on cub production and survival. Much of what is known about the feeding ecology of bears is based on analyses of tissues collected from capture-based research efforts, harvested animals or non-invasive approaches. However, inference about diet from hair has been limited by a lack of quantitative data on the timing of the moult and hair growth rates. We conducted a study to develop and test two methods of quantifying …
The Prize Biter Of Modernity? Investigating Killer Whale Bite Mechanics, Daniel Hong
The Prize Biter Of Modernity? Investigating Killer Whale Bite Mechanics, Daniel Hong
West Chester University Graduate Theses, Dissertations, and Final Projects
Although the killer whale (Orcinus orca) is a cosmopolitan apex predator, very little work has been done on its feeding biomechanics. Here I use finite element analysis to investigate the mandibular stiffness of a juvenile male Bigg’s killer whale, and compare it to that of the false killer whale (Pseudorca crassidens) and the saltwater crocodile (Crocodylus porosus). Full mandibles and left and right halves of mandibles are simulated under FEA. I also estimate the total jaw muscle force produced by the Bigg’s killer whale using the dry skull method. The killer whale lacks an …
Investigating The Relationships Between Biotic And Abiotic Factors Affecting Migratory Behavior In Westslope Cutthroat Trout Within The Pend Oreille System, Garrett M. Duncan
Investigating The Relationships Between Biotic And Abiotic Factors Affecting Migratory Behavior In Westslope Cutthroat Trout Within The Pend Oreille System, Garrett M. Duncan
EWU Masters Thesis Collection
Salmonids express several different life histories from migrating to oceans and back or staying in their natal streams as a resident. Migratory individuals are a vital part of the overall success of populations due to the potential for increased genetic diversity and increased fecundity they offer over resident individuals. This research was completed in three basins including Priest River, Pack River, and Lightning Creek which are all connected to the Pend Oreille Basin and are important producers of Westslope Cutthroat Trout (Oncorhynchus clarkii lewisi). Thirty-nine sites were sampled between the three basins. Results were compared between these tributaries that have …
Letter From The Dean, Jeff Edwards
Letter From The Dean, Jeff Edwards
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
No abstract provided.
Assessing The Prevalence Of Ehrlichia Spp. In Ticks Associated With Canids In Arkansas, Eva Berryhill, Kelly Loftin
Assessing The Prevalence Of Ehrlichia Spp. In Ticks Associated With Canids In Arkansas, Eva Berryhill, Kelly Loftin
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
Canine ehrlichiosis is a common disease seen in dogs. The southeastern United States has been identified as an area of high prevalence, including Arkansas. Changes in climate, household income, and tick population are factors contributing to high prevalence. Canine ehrlichiosis is caused by three Ehrlichia spp., which are E. canis, E. ewingii, and E. chaffeensis. Ticks transmit the pathogen, specifically Rhipicephalus sanguineus, Dermacentor variabilis, and Amblyomma americanum. For this study, the goal was to determine the prevalence of Ehrlichia spp. in Arkansas that causes canine ehrlichiosis. Ticks were collected from host canines and environmental …
Monograph Available: Urban Coyotes As A Source Of Conflict With Humans: An Evaluation Of Common Management Practices
Human–Wildlife Interactions
This is an announcement about the published monograph titled "Urban Coyotes as a Source of Conflict with Humans: An Evaluation of Common Management Practices." This publication, part of the HWI Monograph Series, is online and open access.
Epigenetic Potential Remains Stable Over 100 Years After Introduction, Danielle Dawkins
Epigenetic Potential Remains Stable Over 100 Years After Introduction, Danielle Dawkins
College of Graduate Studies: Theses & Dissertations
Introduced species provide important insights into organismal behavioral responses. Epigenetic mechanisms are one of the various ways in which behaviors can be modified, particularly through DNA methylation. Through such mechanisms, phenotypic changes can occur in response to environmental changes, allowing an organism to be more plastic. Epigenetic potential (EP) represents the capacity for an organism to be epigenetically modified, and can be measured by counting CpG motifs; cytosine adjacent to guanine within a DNA sequence. Phenotypic plasticity as it relates to EP has been studied extensively in the house sparrow (Passer domesticus), a globally introduced songbird. It is …
Comparative Study Of Single Imputation Techniques For The Prediction Of Missing Dairy Data, Ahmed M. Gad Prof, Ahmed Abdelhakim Ahmed Mr, Eman Manaa Prof, Basant Shafik Dr, Sakr Mostafa Prof
Comparative Study Of Single Imputation Techniques For The Prediction Of Missing Dairy Data, Ahmed M. Gad Prof, Ahmed Abdelhakim Ahmed Mr, Eman Manaa Prof, Basant Shafik Dr, Sakr Mostafa Prof
Business Administration
Dairy farm records are a crucial component of effective livestock business management. Record analysis allows a farm’s owner to make informed decisions. Incomplete records are less useful for data analysis, so it's important to handle missing values correctly. This study compares different imputation methods for handling missing values in a dataset of dairy records comprising 997 records collected from 234 cows between 2012 and 2022. The dataset was screened against records with missing values and then deleted, resulting in 858 observations from 200 animals. There were missing values in two variables, with a missing percentage of 13.9%: days in milk …
Habitat Loss And Its Behavioral Effects On A Female Gray Hawk In The Lower Rio Grande Valley Of Texas, Usa, Michael T. Stewart, Ashley M. Tanner, Brian A. Millsap, William S. Clark
Habitat Loss And Its Behavioral Effects On A Female Gray Hawk In The Lower Rio Grande Valley Of Texas, Usa, Michael T. Stewart, Ashley M. Tanner, Brian A. Millsap, William S. Clark
Human–Wildlife Interactions
Habitat loss is a major threat to wildlife worldwide. While habitat loss is commonly associated with the fragmentation, degradation, or destruction of large tracts of suitable space, habitat loss in urban environments can be much more incremental and nuanced. We tracked an adult female gray hawk (Buteo plagiatus) in the Lower Rio Grande Valley of Texas, USA, before and after 70% of the trees (7.6 ha) were cleared from the core of the nesting territory. While the hawk’s overall activity budget between within-patch and exploratory movements was unchanged, the hawk undertook substantially longer exploratory movements after the trees …
Spatiotemporal Variability In Invasive Carp Spawning Activity In Two Midwestern River Basins, Braden A. Whisler
Spatiotemporal Variability In Invasive Carp Spawning Activity In Two Midwestern River Basins, Braden A. Whisler
Masters Theses
Across the United States, fisheries managers and agencies continue to battle the spread of invasive bigheaded carp (Hypophthalmichthys spp.). Although previous research has identified environmental parameters such as temperature and flow to be triggers of spawning activity, many of these studies focus on larger rivers, rarely focusing on the specific thresholds or timing of when a spawning event takes place. I utilized the differing hydrology of the Illinois and Wabash Rivers and their tributaries to examine the differences in environmental parameters needed to trigger bigheaded carp reproduction. Three mainstem sites and three tributaries of both basins were sampled …
Pharmacological Safety Profiling Of Nigella Sativa (Ns) And Hibiscus Sabdariffa (Hs) Extracts In Zebrafish (Danio Rerio) Embryos: An Integrative Vet-Pharmacotoxicological Evaluation Of Lc₅₀, Teratogenicity, And Developmental Outcomes, Ahmed E. A. Mostafa
Mansoura Veterinary Medical Journal
Herbal medicines are deeply rooted in Egyptian and Middle Eastern traditions, with Nigella sativa (NS) and Hibiscus sabdariffa (HS) being among the most widely consumed remedies. Despite extensive pharmacological evidence of their therapeutic benefits, systematic safety validation remains limited. Conventional toxicity assessments predominantly rely on rodents and higher vertebrates, which are costly, time-consuming, and ethically challenging. This gap necessitates alternative models that align with the 3R principles (Reduction, Replacement, Refinement). In this study, the zebrafish (Danio rerio) embryo model was employed to evaluate the developmental toxicity and median lethal concentration (LC50) of NS and HS aqueous extracts. Zebrafish embryos at …