Animal Sciences Commons^™

Early Events In Japanese Encephalitis Virus Infection: Viral Entry, Sang-Im Yun, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Japanese encephalitis virus (JEV), a mosquito-borne zoonotic flavivirus, is an enveloped positive-strand RNA virus that can cause a spectrum of clinical manifestations, ranging from mild febrile illness to severe neuroinvasive disease. Today, several killed and live vaccines are available in different parts of the globe for use in humans to prevent JEV-induced diseases, yet no antivirals are available to treat JEV-associated diseases. Despite the progress made in vaccine research and development, JEV is still a major public health problem in southern, eastern, and southeastern Asia, as well as northern Oceania, with the potential to become an emerging global pathogen. In …

Work-Type Influences Perceived Livestock Herding Success In Australian Working Kelpies, Jonathan B. Early, Elizabeth R. Arnott, Lisa J. Mascord, Diane Van Rooy, Paul Mcgreevy, Claire M. Wade

Genetics Collection

Background

Working dog handlers and breeders have very different behavioural requirements in the animals that they employ for managing livestock. The Australian Working Kelpie breed may be used in several working contexts, notably yards, paddocks and a combination of both. The working context influences the skillsets required and gives rise to three corresponding work-types: Yard, Paddock and Utility Kelpies. In particular, dogs used for working stock in the confines of yards and trucks interact with stock more forcefully than those mustering in larger areas (paddocks) where they can herd stock effectively from a greater distance. This article explores owner assessments …

Using Local Fishery Monitoring To Understand Small-Scale Coastal Fisheries In Tanzania, Matthew Robertson

LSU Master's Theses

Nearshore marine fisheries provide the main source of protein for nearly 9 million people in the coastal villages of Tanzania, yet for decades the fisheries have shown signs of overexploitation. These fisheries are small-scale and co-managed by local coastal communities in groups known as Beach Management Units (BMUs). BMUs monitor individual fishing trip data (e.g. gear, vessel, taxa); however, these data have only been analyzed in nationally aggregated statistics and to our knowledge, are not presently used in management decision making. The present thesis aimed to identify the forms of data and information that local fishery monitoring can record about …

Robert James Baker (1942-2018), Obituary, Hugh H. Genoways, Robert D. Bradley, David J. Schmidly, Lisa C. Bradley, James J. Bull, Karen Mcbee, Meredith J. Hamilton, Peter A. Larsen

University of Nebraska State Museum: Mammalogy Papers

First paragraph:

On 30 March 2018, the science of mammalogy and the American Society of Mammalogists lost one of the most influential figures of the last half-century. Robert James Baker died quietly at his home in Lubbock, Texas (Fig. 1). He was born on 8 April 1942 to James Simeon Baker and Laura Cooper in Warren, Arkansas. His father was killed during World War II and his mother remarried, resulting in his growing up with six half-siblings. According to Robert’s autobiography in Going afield (330—number refers to specific publication in “Bibliography”), he spent a good deal of his youth with …

Management Implications Of Sex-Specific Habitat Use By Nycticeius Humeralis In North-Central Arkansas, Megan Lynn Buckley

Student Theses and Dissertations

The goal of this study was to evaluate evening bat (Nycticeius humeralis) habitat use and provide a proxy strategy to evaluate habitat use by other species. I used the software Maximum Entropy to evaluate habitat suitability models for evening bats using three different modeling strategies; percent variable contribution, the jackknife test, and variable response curves. The predictors included in the models were 10 land use land cover classes, four forest management strategies, slope, and the normalized difference vegetation index (NDVI). The results of this research showed that distance to burned stands and proximity to edge habitat are the most important …

Functional Genomics And Immunologic Tools: The Impact Of Viral And Host Genetic Variations On The Outcome Of Zika Virus Infection, Sang-Im Yun, Byung-Hak Song, Jordan C. Frank, Justin G. Julander, Aaron L. Olsen, Irina A. Polejaeva, Christopher J. Davies, Kenneth L. White, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) causes no-to-mild symptoms or severe neurological disorders. To investigate the importance of viral and host genetic variations in determining ZIKV infection outcomes, we created three full-length infectious cDNA clones as bacterial artificial chromosomes for each of three spatiotemporally distinct and genetically divergent ZIKVs: MR-766 (Uganda, 1947), P6-740 (Malaysia, 1966), and PRVABC-59 (Puerto Rico, 2015). Using the three molecularly cloned ZIKVs, together with 13 ZIKV region-specific polyclonal antibodies covering nearly the entire viral protein-coding region, we made three conceptual advances: (i) We created a comprehensive genome-wide portrait of ZIKV gene products and their related species, with several previously …

Breeding Bird Response To Post Oak Savanna Restoration Seven Years Post Management In Eastern Texas, Courtney Mcinnerney

Electronic Theses and Dissertations

Oak savannas were once an abundant vegetation type in the Midwestern United States that have now declined to <1% of their original distribution. Historically, natural disturbances such as periodic fire and grazing maintained oak savannas, but these have been reduced or eliminated, resulting in woody encroachment and subsequent habitat loss and degradation. In 2009-10, a baseline, pre-restoration study was completed to determine vegetation characteristics, breeding bird abundances, nest success, and nest site selection at the Gus Engeling Wildlife Management Area (GEWMA) in eastern Texas. The results showed a lack of savanna vegetation structure on degraded sites and few savanna or grassland obligate bird species. The goal of this study was to determine how breeding birds of oak savanna vegetation types in eastern Texas respond to restoration effects 7 years after initial management. Post-restoration surveys completed in 2016-17 showed a change in avian assemblages from a more woodland dominated community to grassland/savanna community. The presence and breeding of savanna obligate species dickcissel (Spiza americana) and lark sparrow (Chondestes grammacus) indicates that the restoration was successful. The presence of savanna species can be linked to the herbaceous vegetation that was restored to more closely resemble historic oak savanna structure and can quantify the success of restoration efforts.

An Investigation Of Vocal Learning Propensity In Zebra Finch (Taeniopygia Guttata), Tatsuya Hayashi

Theses and Dissertations

The investigator attempted to predict the vocal learning propensity from either family pedigree or early vocal babbling (subsong). Pedigree trees suggest the possibility that vocal learning ability may depend on family. We failed to predict vocal learning propensity from subsong, but suggested that subsong may influence vocal learning outcome.

Bubble Stream Production By Belugas (Delphinapterus Leucas), Megan Slack

Theses

Bubble stream production in belugas has been poorly characterized and its function is not well understood. I examined behavioral states when producing bubble streams (“bubbling”), and when bubbling calls, to determine whether bubbling was significantly associated with a particular call category or behavioral state. Using 19 hours of video and audio recordings collected over a two-day period, I quantified bubble streams of a 4-month old calf and an unrelated adult female housed together. Based on the overall activity budgets and pool of vocalizations for both animals, I calculated the expected counts of bubble streams with and without vocalizations, assuming that …

The Paper Zoo: 500 Years Of Animals In Art By Charlotte Sleigh, Gina M. Granter

The Goose

Review of Charlotte Sleigh's The Paper Zoo: 500 Years of Animals in Art.

Establishing Hematology And Serum Chemistry Reference Intervals For Wild Hawaiian Monk Seals (Neomonachus Schauinslandi), Angela C. Kaufman, Stacie J. Robinson, Dori L. Borjesson, Michelle Barbieri, Charles L. Littnan

United States Department of Commerce: Staff Publications

Hematology and serum chemistry reference intervals have been previously established for the endangered Hawaiian monk seal (Neomonachus schauinslandi) as an imperative measure for health assessments. Monitoring the health of the wild population depends upon reference intervals that are context specific; hence we developed reference intervals from fresh samples, as opposed to frozen, from wild monk seals. This study builds on the number of parameters from previous efforts by using samples collected between 2004 and 2015 from wild monk seals. Blood samples were analyzed by a single veterinary diagnostic laboratory within 24 hr of collection from apparently healthy, wild …

Does Stat5a Have An Effect On Bmal1 Levels In Mammary Epithelial Cells?, Clare E. Aduwari, Aridany Suarez-Trujillo, Karen I. Plaut, Theresa M. Casey

The Summer Undergraduate Research Fellowship (SURF) Symposium

The mammary gland is a very important organ for reproduction in mammals because it produces milk which serves as the primary source of nutrients for newly-born offspring. Previous studies suggest that its development is regulated by circadian clocks, biochemical oscillators that generate circadian rhythms (the body’s internal clock). The circadian system plays a major role in homeostasis, coordinating the body’s internal physiology and synchronizing it with the external environment. Our lab showed that levels of the BMAL1 protein, a core clock component, increased in the mammary gland at the onset of lactation. Treatment of mammary epithelial cells (HC11) with the …

Immunogenicity And Protection After Vaccination With A Modified Vaccinia Virus Ankara-Vectored Yellow Fever Vaccine In The Hamster Model, Justin G. Julander, Marco Testori, Cédric Cheminay, Ariane Volkmann

Animal, Dairy, and Veterinary Science Faculty Publications

The highly efficacious live-attenuated 17D yellow fever (YF) vaccine is occasionally associated with rare life-threatening adverse events. Modified vaccinia virus Ankara (MVA), a non-replicating poxvirus, has been used as a vaccine platform to safely deliver various antigens. A MVA-based YF vaccine (MVA-BN-YF) was tested with and without a non-mineral oil adjuvant in a hamster model of lethal YF disease and protective efficacy of this vaccine was compared with the 17D vaccine. The vaccine candidate MVA-BN-YF generated a protective response in hamsters infected with YFV that was comparable to protection by the live 17D vaccine. Similar levels of neutralizing antibody were …

Biodiversity And Conservation Study Of The St. Norbert Abbey, De Pere, Wisconsin - 2017 Annual Report, Mikaela Nowak, Mackenzie Hennes, Adam L. Brandt

Student Creative and Scholarly Works

Long term biological studies are rare but incredibly valuable for examining natural phenomena. Data collected over many years or decades allows for analyses of trends that would never be apparent in a single season. The research presented here is for the completion of the first field season in a long term study analyzing biodiversity trends at the St. Norbert Abbey. Using visual searches, live trapping, and trail cameras, the biodiversity and abundance of species was examined. Special emphasis was placed on small mammals, though other species were observed and documented. The goals of this project were to (1) provide preliminary …

Haemosporidian Parasite Community In Migrating Bobolinks On The Galapagos Islands, Noah G. Perlut, Patricia G. Parker, Rosalind B. Renfrew, Maricruz Jaramillo

Environmental Studies Faculty Publications

Bobolinks (Dolichonyx oryzivorus) migrate from their breeding grounds in North America to their wintering grounds in South America during the fall each year. A small number of Bobolinks stop temporarily in Galapagos, and potentially carry parasites. On the North American breeding grounds, Bobolinks carry a least two of the four Plasmodium lineages recently detected in resident Galapagos birds. We hypothesized that Bobolinks carried these parasites to Galapagos, where they were bitten by mosquitoes that then transmitted the parasites to resident birds. The haemosporidian parasite community in 44% of the Bobolinks we captured was consistent with those on their …

Age-Specific Differences In Fat Reserves And Migratory Passage Of Setophaga Striata (Blackpoll Warbler), Emily N. Filiberti, Noah G. Perlut

Environmental Studies Faculty Publications

Adequate fat reserves are vital for long non-stop transatlantic avian-migration movements, such as those made by Setophaga striata (Blackpoll Warbler). Over a 5-y period, we studied differences in preparedness (determined by presence of fat content and arrival time at stopover locations) between hatch-year (HY) and after hatch-year (AHY) Blackpoll Warblers at 3 stopover sites (Hancock and York counties in Maine, and Plymouth County in Massachusetts) covering 2.65° latitude along the Gulf of Maine. Migration timing varied across a latitudinal gradient and between age classes. In September, AHY Blackpoll Warblers were more abundant in the northern and central counties, but HY …

Kentucky Warbler (Vol. 94, No. 3), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Decision Tree And Rapid Appraisal Methodology For New Fisheries, Anthony M. Hart

Fisheries Research Reports

The objective of this report is to provide a process (RAM – Rapid Appraisal Methodology) for assessing proposals for new fisheries. If adopted, the methodology could extend to a potential new initiative, referred to as WildCulture in this report. The RAM process is designed to be a modern, forward thinking, opportunity creating instrument for enhancing seafood production from renewable aquatic resources.

Growth Of Pelagic Age-0 Yellow Perch In Relation To Hatch Dates And Water Temperature In North-Central Minnesota Lakes, Ethan Karppinen

Biology Graduate Theses

Exposure to consistent water warming rates and access to abundant populations of edible sized zooplankton affects growth and increases the probability of survival for newly hatched Yellow Perch Perca flavescens. Seven lakes with varying physical characteristics were selected in north-central Minnesota to study abiotic (water temperature) and biotic (zooplankton community and fish growth) progressions during the spring and early summer (April-June). Mean water temperatures from ice-out to 28 June (11.98-14.62 °C) had significant relationships with mean depth (P < 0.01, R? = 0.85), max depth (P < 0.01, R? = 0.73), and total volume (P < 0.01, R2 = 0.54). Hatch periods ranged from 13 to 19 days in 2015 and 13 to 25 days in 2016. Cumulative degree-days (CDD) were used to predict Yellow Perch hatch periods and explain growth. CDD predicted 21-81% of hatch periods in 2015 and 11-50% in 2016. Mean predicted total lengths at 28 June were 19.65-30.41 mm in 2015 and 20.88-28.54 mm in 2016. There was a significant relationship between CDD and predicted total length (R? = 0.62, P < 0.01) to explain differences in Yellow Perch total length while inhabiting the pelagic zone. Observed gape measurements were highly correlated with predicted gapes from the Schael formula (R? = 0.89, P < 0.01) that was used to assess the availability of edible sized zooplankton for age-0 Yellow Perch in each lake. No predator-prey mismatches were found and earlier hatch dates produced age-0 Yellow Perch with greater predicted total lengths in late June on the majority of lakes. This suggests that earlier hatched Yellow perch have an advantage over later hatched Yellow Perch in attaining greater lengths by late June. Our study provides information on a variety of physical and biological progressions in lakes, which may be used for fisheries management practices in the future.

A Non-Recoverable Hybridoma Limits The Production Of Monoclonal Antibodies Against Bovine Trophoblast Non-Classical Nc3*00101 Protein, Parveen Parasar, Amanda Wilhelm, William C. Davis, Kenneth L. White, Christopher J. Davies

Animal, Dairy, and Veterinary Science Faculty Publications

The non-classical MHC class I (MHC-Ib) proteins are important modulators of immune system during pregnancy favoring survival of the fetus. Contrary to ubiquitously expressed classical MHC-I proteins, MHC-Ib proteins are oligomorphic, and expressed in specific cell/tissue types thus minimizing maternal immune-mediated rejection of fetal-allograft and a successful pregnancy. A unique characteristic of MHC-Ib glycoproteins is expression of surface and soluble isoforms due to alternative splicing phenomenon. Bovine fetal trophoblast cells, during the third trimester of pregnancy, express non-classical bovine leukocyte antigen class Ib (BoLA-Ib) antigens. BoLA-NC3*00101, is a non-classical class I allele from cattle AH11 …

Acute Exposure Of Primary Rat Soleus Muscle To Zilpaterol Hcl (Β2 Adrenergic Agonist), Tnfα, Or Il-6 In Culture Increases Glucose Oxidation Rates Independent Of The Impact On Insulin Signaling Or Glucose Uptake, Caitlin N. Cadaret, Kristin A. Beede, Hannah E. Riley, Dustin T. Yates

Department of Animal Science: Faculty Publications

Recent studies show that adrenergic agonists and inflammatory cytokines can stimulate skeletal muscle glucose uptake, but it is unclear if glucose oxidation is similarly increased. Thus, the objective of this study was to determine the effects of ractopamine HCl (β1 agonist), zilpaterol HCl (β2 agonist), TNFα, and IL-6 on glucose uptake and oxidation rates in unstimulated and insulin-stimulated soleus muscle strips from adult Sprague-Dawley rats. Effects on phosphorylation of Akt (phospho-Akt), p38 MAPK (phospho-p38), and p44/42 MAPK (phospho-p44/42) was also determined. Incubation with insulin increased (P < 0.05) glucose uptake by ~47%, glucose oxidation by ~32%, and phospho-Akt by ~238%. Insulin also increased (P < 0.05) phospho-p38, but only after 2 hours in incubation. Muscle incubated with β2 agonist alone exhibited ~20% less (P < 0.05) glucose uptake but ~32% greater (P < 0.05) glucose oxidation than unstimulated muscle. Moreover, co-incubation with insulin + β2 agonist increased (P < 0.05) glucose oxidation and phospho-Akt compared to insulin alone. Conversely, β1 agonist did not appear to affect basal or insulin-stimulated glucose metabolism, and neither β agonist affected phospho-p44/42. TNFα and IL-6 increased (P < 0.05) glucose oxidation by ~23% and ~33%, respectively, in the absence of insulin. This coincided with increased (P < 0.05) phospho-p38 and phospho-p44/42 but not phospho-Akt. Furthermore, co-incubation of muscle with insulin + either cytokine yielded glucose oxidation rates that were similar to insulin alone, despite lower (P < 0.05) phospho-Akt. Importantly, cytokine-mediated increases in glucose oxidation rates were not concomitant with greater glucose uptake. These results show that acute β2 adrenergic stimulation, but not β1 stimulation, directly increases fractional glucose oxidation in the absence of insulin and synergistically increases glucose oxidation when combined with insulin. The cytokines, TNFα and IL-6, likewise directly increased glucose oxidation in the absence of insulin, but were not additive in combination with insulin and in fact appeared to disrupt Akt-mediated insulin signaling. Rather, cytokines appear to be acting through MAPKs to elicit effects on glucose oxidation. Regardless, stimulation of glucose oxidation by these key stress factors did not rely upon greater glucose uptake, which may promote metabolic efficiency during acute stress by increasing fractional glucose oxidation without increasing total glucose consumption by muscle.

Evaluation Of Growth Promoting Implant Strategies And Days On Feed On Finishing Heifer Performance And Evaluation Of Optimal Reimplant Times For Finishing Heifers And Steers, Caitlin A. Ohnoutka

Department of Animal Science: Dissertations, Theses, and Student Research

Two experiments evaluated the effects of delayed, long-acting coated implants or uncoated implants to non-implanted heifers fed for constant or varying days on feed. In Exp. 1, heifers were implanted with either Revalor-XH on d 1, Revalor-200 on d 1, Revalor-XR on d 1, or Revalor-200 on d 70 compared to non-implanted control heifers when fed for an average of 198 d. In Exp. 2, heifers were implanted with Revalor-200 on d 1 and reimplanted with Revalor-200 on d 100, Revalor-XH on d 1, or not implanted and fed for different days on feed: 151, 165, 179, or 193. Implanting …

Effect Of Corn Silage Harvest, Hybrid, And Concentration On Performance In Growing And Finishing Beef Cattle, Fred H. Hilscher Jr.

Department of Animal Science: Dissertations, Theses, and Student Research

Six studies were conducted to evaluate effects of corn silage harvest, hybrid, and concentration in growing and finishing diets. Experiment 1, evaluated corn silage DM (37 or 43%) and replacing corn grain with silage (15 or 45% of diet DM) in finishing diets. Experiment 2, evaluated corn silage DM (37 or 43%) and response to rumen undegradable protein (RUP) supplementation in growing diets. Experiment 3, evaluated nutrient digestibility of 37 or 43% DM corn silage at two different intakes. Experiment 4, 5, and 6 evaluated three corn silage hybrids: a standard hybrid control (CON), a brown midrib (bm3) …

The Mayfly Newsletter, Donna J. Giberson

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.

Detecting A Probiotic Product Within The Gut Of Broiler Chickens, Anneka Pisula

Master's Theses

As of January 2017, the U.S. poultry industry banned the use of antibiotics and now relies on alternatives such as probiotics to help protect animal health. Although probiotic use is not a new concept in the poultry industry, identifying the best combination of bacterial strains to generate an effective probiotic formula requires further investigation. This study aimed to detect a probiotic product of four bacterial strains (Pedioccoccus acidilactici, Pediococcus pentosaceus, Lactobacillus plantarum, and Bacillus subtilis) in a feeding trial with broiler chickens. Birds given the probiotic were predicted to show an improved growth performance with …

Effects Of Polydatin On In Vitro Bovine Embryo Developmental Competence, Metabolism, And Cryopreservation, Corie Marie Owen

Master's Theses

Bovine in vitro produced embryos suffer from poor developmental competence and altered metabolism which hinders their cryotolerance. Overall, the goals of this thesis were to improve oocyte and embryo culture with the antioxidant polydatin and to optimize slow freezing procedures. This thesis was designed as three experiments, and in each experiment, oocytes were aspirated from abattoir ovaries, matured for 23h, fertilized with semen from 1 of 3 bulls, and cultured in synthetic oviductal (SOF) based medium (SCF1) in 38.5 °C in 5% O₂, 5% CO₂ and 90% N₂. Stage 7 blastocysts were stained with Nile …

Bird Hazard Mitigation Training For General Aviation Pilots - A Prospective Research Study, Flavio A. C. Mendonca, Thomas Q. Carney

Publications

• General aviation industry
• 446,000 aircraft worldwide
• 211,000 in the U.S.
• Supports $219 billion in total economic output and 1.1 million jobs in the U.S
• Flies approximately 25 million flight hours (U.S.)
• Flies to more than 5,000 public airports
• Primary training ground for most commercial airline pilots

Investigating The Potential Of Plant-Derived Antimicrobials For Controlling Campylobacter Jejuni In Poultry And Poultry Products, Basanta Raj Wagle

Graduate Theses and Dissertations

Campylobacter jejuni infection in humans is strongly associated with the handling and consumption of contaminated poultry products. Interventions reducing C. jejuni contamination in poultry would reduce the risk of subsequent human infections. In the first study, the efficacy of a Generally Recognized as Safe (GRAS) compound, eugenol (EG; derived from cloves), as an antimicrobial dip treatment to reduce C. jejuni in postharvest poultry was evaluated. The antimicrobial efficacy of EG was studied in suspension, emulsion and nanoemulsion delivery systems. EG suspension reduced C. jejuni counts with the greatest reduction of >2.0 Log CFU/sample for the 2% dose of EG (P

Effect Of Morinda Citrifolia (Noni)-Enriched Diet On Hepatic Heat Shock Protein And Lipid Metabolism-Related Genes In Heat Stressed Broiler Chickens, Joshua Flees

Graduate Theses and Dissertations

Heat stress (HS) has been reported to alter fat deposition in broilers, however the underlying molecular mechanisms are not well-defined; therefore, the objectives of the current study were to: (1) determine the effects of acute (2 h) and chronic (3 wk) HS on the expression of key molecular signatures involved in hepatic lipogenic and lipolytic programs; and (2) assess if diet supplementation with dried Noni medicinal plant (0.2% of the diet) modulates these effects. Broilers (n=480 males, 1 d) were randomly assigned to 12 environmental chambers, subjected to two environmental conditions (HS at 35°C vs. thermoneutral condition [TN] at 24°C) …

Investigation Of Bacterial Taxa As Biomarkers Of Fescue Toxicosis And Heat Stress In Grazing Beef Cows, Sarah Katherine Chewning

Graduate Theses and Dissertations

Fescue toxicosis in grazing beef cattle is caused by consumption of Ergot alkaloids associated with tall fescue (toxic fescue) and is responsible for substantial economic loss to the U.S. beef industry. Cattle consuming toxic fescue suffer adverse physiological responses, such as: hyperthermia, increased respiration rate (RR), poor reproduction and growth performance. Other adverse responses to fescue toxins include the retention of a winter hair coat and vasoconstriction in the extremities, which can lead to tissue necrosis. Identifying cattle with reduced susceptibility to fescue toxins would allow for efficient use of fescue pastures; thus, the objectives of this thesis was to: …