Agriculture Commons^™

Transformation And Gene Editing In The Bioenergy Grass Miscanthus, Anthony Trieu, Mohammad B. Belaff, Pradeepa Hirannaiah, Shilpa Manjunatha, Rebekah Wood, Yokshitha Bathula, Rebecca L. Billingsley, Anjali Arpan, Erik J. Sacks, Thomas E. Clemente, Stephen P. Moose, Nancy A. Reichert, Kankshita Swaminathan

Department of Agronomy and Horticulture: Faculty Publications

Background: Miscanthus, a C4 member of Poaceae, is a promising perennial crop for bioenergy, renewable bioproducts, and carbon sequestration. Species of interest include nothospecies M. x giganteus and its parental species M. sacchariforus and M. sinensis. Use of biotechnology-based procedures to genetically improve Miscanthus, to date, have only included plant transformation procedures for introduction of exogenous genes into the host genome at random, non-targeted sites.

Results: We developed gene editing procedures for Miscanthus using CRISPR/Cas9 that enabled the mutation of a specific (targeted) endogenous gene to knock out its function. Classified as paleo-allopolyploids (duplicated ancient …

Evaluation Of The Effect Of Kiwifruit Cultivars On The Quality Of Kiwifruit Chips, An Chuan-Hui, Huang Wen-Jun, Zhong Cai-Hong, He Jian-Jun, Chen Xue-Ling, Zhang Qi

Food and Machinery

Objective: The aim of this study was to analysis the effect of cultivars on the adaptability of kiwifruit processing to chips while six cultivars of kiwifruit were used as the raw material. Methods: Kiwifruit chips were prepared by the method of vacuum freeze-dried. Sensory evaluation and nine indexes such as the yield of kiwifruit chips, water-soluble pectin, acid-soluble pectin, soluble sugar, total acid content, sugar-acid ratio. ascorbic acid content, hardness and crispness of kiwifruit chips were analyzed. Results: There were significantly differences among yield, nutrient content and sensory indicators in kiwifruit chips while the kiwifruit chips were …

The Development Process, Implementation Characteristics And Experience Enlightenment Of Infant Food Education In Japan, Shi Gui-Hong, Feng Jiang-Ying

Food and Machinery

This paper systematically combs the development process and implementation characteristics of Japanese infants food education, and puts forward that China can establish a scientific and perfect Chinese infants food education system on the basis of learning from the mature experience of Japanese infants food education, starting from the creation of infants characteristic food education courses, strengthening the training of food education teachers, building a food education coordination mechanism, inheriting excellent food culture and improving food education laws and regulations.

Aspen Leaves As A "Chemical Landscape" For Fungal Endophyte Diversity - Can Nitrogen And Herbivory Shape The Community Composition In Controlled Conditions?, Johanna Witzell, Vicki Huizu Guo Decker, Marta Agostinelli, Carmen Romeralo Tapia, Michelle Cleary, Benedicte Riber Albrectsen

Aspen Bibliography

The endophytic microbiome may influence the ecological performance of plants, including forest trees. Various abiotic and biotic factors may shape the endophyte communities directly but also indirectly, by modifying the quality of host plants as a substrate. We hypothesized that potentially antifungal or fungistatic condensed tannins (CTs) would determine the quality of aspen (Populus tremula) leaves as a substrate for endophytic fungi. By subjecting the plants to nitrogen fertilization (N) or herbivory (H; leaf beetles) we aimed to change the internal “chemical landscape”, especially the CT levels, in aspen leaves. We expected that this would lead to changes in the …

Easy Calibration For Backpack And Canister (Handheld) Sprayers, Cody Zesiger, Kalen Taylor, Corey Ransom, Earl Creech, Matt Yost

All Current Publications

When applying pesticides, fertilizers, and other chemicals, the applicator should know the amount of chemical applied to the treated area. Calibration is a method an applicator can use to measure and set the application rate. If sprayers are not properly calibrated, it can result in over or under application of chemicals, causing damage, reduced pest control, and potential safety concerns. Making the effort to properly mix chemicals and calibrate sprayers can save money and increase treatment effectiveness. This fact sheet provides simple guidelines for calibrating backpack and canister sprayers.

Easy Calibration For Boom Sprayers, Cody Zesiger, Kalen Taylor, Corey Ransom, Earl Creech, Matt Yost

All Current Publications

Calibrating spray equipment is an important first step when applying pesticides, fertilizers, and other chemicals. If nozzles are not properly calibrated, it can result in over or under application of chemicals, causing crop damage, reduced pest control, and potential safety concerns. Making the effort to properly mix chemicals and calibrate sprayers can save money and headaches in the long run. This fact sheet provides simple guidelines for calibrating boom sprayers.

New Semi-Dwarfing Alleles With Increased Coleoptile Length By Gene Editing Of Gibberellin 3-Oxidase 1 Using Crispr-Cas9 In Barley (Hordeum Vulgare L.), Jingye Cheng, Camilla Hill, Yong Han, Tianhua He, Xingguo Ye, Sergey Shabala, Ganggang Guo, Meixue Zhou, Ke Wang, Chengdao Li

Grain and Other Field Crops Research Articles

The green revolution was based on genetic modification of the gibberellin (GA) hormone system with “dwarfing” gene mutations that reduces GA signals, conferring shorter stature, thus enabling plant adaptation to modern farming conditions. Strong GA-related mutants with shorter stature often have reduced coleoptile length, discounting yield gain due to their unsatisfactory seedling emergence under drought conditions. Here we present gibberellin (GA) 3-oxidase1 (GA3ox1) as an alternative semi-dwarfing gene in barley that combines an optimal reduction in plant height without restricting coleoptile and seedling growth. Using large-scale field trials with an extensive collection of barley accessions, we showed that a natural …

Yield Prediction Through Integration Of Genetic, Environment, And Management Data Through Deep Learning, Daniel R. Kick, Jason G. Wallace, James C. Schnable, Judith M. Kolkman, Barış Alaca, Timothy M. Beissinger, Jode Edwards, David Ertl, Sherry Flint-Garcia, Joseph L. Gage, Candice N. Hirsch, Joseph E. Knoll, Natalia De Leon, Dayane C. Lima, Danilo E. Moreta, Maninder P. Singh, Addie Thompson, Teclemariam Weldekidan, Jacob D. Washburn

Department of Agronomy and Horticulture: Faculty Publications

Accurate prediction of the phenotypic outcomes produced by different combinations of genotypes, environments, and management interventions remains a key goal in biology with direct applications to agriculture, research, and conservation. The past decades have seen an expansion of new methods applied toward this goal. Here we predict maize yield using deep neural networks, compare the efficacy of 2 model development methods, and contextualize model performance using conventional linear and machine learning models. We examine the usefulness of incorporating interactions between disparate data types. We find deep learning and best linear unbiased predictor (BLUP) models with interactions had the best overall …

Pests Of Hemp In Utah, Marion Murray, Macahl Evans, Claudia Nischwitz, Austin Taylor, Cody Zesiger

All Current Publications

This guide includes information on insect and disease pests of hemp, organized by pest group. Much of the content is a result of a two-year arthropod and plant disease survey of field-grown hemp operations in northern Utah. Some pests included in this guide were not found in that survey but may be likely to occur in the future. Beneficials and natural enemies are also covered, and information on pesticides is included.

Anthelmintic Resistance In Sheep Across Ireland And The Uk: A Literature Review Of The In Vivo Versus In Vitro Methods, Megan Tumulty

SURE Journal: Science Undergraduate Research Experience Journal

Background: The animal welfare and production consequences of gastrointestinal nematode infections, namely Nematodirus battus, on sheep are compelling. The control of gastrointestinal infections has been heavily reliant on the administration of anthelmintics since their introduction into mainstream use in 1960. However, their frequent and often excessive use has resulted in anthelmintic resistance being reported extensively in several gastrointestinal nematode species. The prevalence of anthelmintic resistance as well as the increase in the number of cases of multiple-drug resistance in nematode populations now poses substantial hindrance on the viability of sheep production systems. However, despite the threat N. battus poses …

Weakly-Supervised Learning Method For The Recognition Of Potato Leaf Diseases, Junde Chen, Xiaofang Deng, Yuxin Wen, Weirong Chen, Adnan Zeb, Defu Zhang

Engineering Faculty Articles and Research

As a crucial food crop, potatoes are highly consumed worldwide, while they are also susceptible to being infected by diverse diseases. Early detection and diagnosis can prevent the epidemic of plant diseases and raise crop yields. To this end, this study proposed a weakly-supervised learning approach for the identification of potato plant diseases. The foundation network was applied with the lightweight MobileNet V2, and to enhance the learning ability for minute lesion features, we modified the existing MobileNet-V2 architecture using the fine-tuning approach conducted by transfer learning. Then, the atrous convolution along with the SPP module was embedded into the …

Genetic Variation In Common Bunt Resistance In Synthetic Hexaploid Wheat, Amira M. I. Mourad, Alexey Morgounov, P. Stephen Baenziger, Samar M. Esmail

Department of Agronomy and Horticulture: Faculty Publications

Common bunt (caused by Tilletia caries and T. Foetida) is a major wheat disease. It occurs frequently in the USA and Turkey and damages grain yield and quality. Seed treatment with fungicides is an effective method to control this disease. However, using fungicides in organic and low-income fields is forbidden, and planting resistant cultivars are preferred. Due to the highly effective use of fungicides, little effort has been put into breeding resistant genotypes. In addition, the genetic diversity for this trait is low in modern wheat germplasm. Synthetic wheat genotypes were reported as an effective source to increase the …

Estimated Impact Of X-Disease And Little Cherry Disease In Washington And Oregon From 2015 To 2020, Cody Molnar, Tianna Dupont, Ashley Thompson

Journal of Extension

X-disease phytoplasma and little cherry virus 1 and 2 have resulted in substantial loss of stone fruit acreage in Washington and Oregon. The extent and financial impact on the industry was not previously known. A Washington and Oregon State University survey documented 238,856 trees equivalent to 974 acres of sweet cherries removed due to X-disease and little cherry disease between 2015 and 2020. Removed trees reduced revenue to the industry by an estimated $30 million in 2020 and $65 million between 2015 and 2020. Over the seven-year re-establishment period estimated lost revenue and establishment costs to growers is an estimated …

Development And Assessment Of A Food Safety Training Program For Farmers’ Market Vendors, Joshua Scheinberg, Rama Radhakrishna, Catherine Cutter

Journal of Extension

Based on results collected through a comprehensive needs assessment of farmers market (FM) vendors in Pennsylvania, the purpose of this study was to develop and pilot-test a customized, food safety training program for FM vendors. A customized 3-hour, in-person, training program was developed and pilot tested. Using pre- and post-test assessment tests through piloted training, the results found participants scores on knowledge questions increased significantly by ~20% (p

Colored Sweet Bell And Tapered Pepper Cultivar Evaluation For High Tunnel Production In West-Central Indiana, 2022, Petrus Langenhoven, Lian Mahorith Duron Alvarado, Eduardo Jose Miranda Oviedo

Midwest Vegetable Trial Reports

Colored sweet bell-shaped and tapered peppers are a staple at the farmers' market. Many small and medium-sized farming operations in Indiana grow this summer crop. High tunnels allow growers to protect their crops and extend their growing season. Sweet peppers, in particular, benefit from the unique growing environment created by the high tunnel. Planting of peppers can start at least 2-4 weeks earlier in the spring, and production can continue into the fall until the first hard freeze. Pepper variety performance data for Indiana is not readily available. We are working hard to change that. Currently, we have evaluated twenty-five …

Understanding Current Labor Shortage And Mechanization In New Jersey Nursery Crop Operations, Hemant Gohil, Timothy Waller, Raul Cabrera

Journal of Extension

We conducted a survey of New Jersey’s nursery industry to understand the current levels of labor shortage, how this has affected specific tasks related to the production capacity of the industry, and the actual level of mechanization. Survey results identified priority areas needing mechanization. There is a need for greater mechanization in almost all areas of nursery production to reduce the reliance on labor and or improve the efficiency of labor. Other Implications related to Extension programming on nursery mechanization are also discussed.

Connecting Diverse Communities Through A 4-H Urban-Rural Experience, Laurie Murrah-Hanson, Brittani Lee, Pamela Bloch

Journal of Extension

Misconceptions regarding agricultural careers are exacerbated by increasing urbanization and are one of many differences in perspective between urban and rural communities. The One Georgia 4-H Urban-Rural Experience addresses this divide through a statewide youth exchange focusing on the agricultural sector. The program includes a multifaceted look at agricultural careers through visits to urban and rural communities. Focus group evaluations revealed the impact on youth through exposure to new careers, increased understanding of the diversity of agriculture, awareness of the interconnected nature of urban and rural communities, and a sense of belonging to a diverse group of youth.

Is There An Economic Advantage To Planting Diverse Summer Annual Forage Mixtures?, Kelly Mercier, Chris Teutsch, Ray Smith, Kenny Burdine, Edwin Ritchey, Eric Vanzant

Journal of Extension

This study examined economic implications of planting summer annual mixtures of grasses, legumes, and forbs at varying nitrogen rates. No differences in yield occurred between the three mixtures, indicating that mixtures with lowest seed cost will be most economical. Applying N resulted in yield increases of 12.26 lb DM per lb N applied. Although yield responses to N were positive, sensitivity analyses showed that applying N resulted in positive net returns only when hay prices were high and N prices were low. When utilization rates are accounted for, enterprise budgets determined grazing to be 18% cheaper to implement than haying.

Effect Of Medium Type And Foliar Spray With Concentrations Of Nano Zinc, Chelated Iron And Normal On The Mineral Content For Strawberry Seedlings Duch. Fragaria×Ananassa, Ahmed Al-Dulaimy, Mustafa Mahmood

Hebron University Research Journal-A (Natural Sciences) - (مجلة جامعة الخليل للبحوث- أ (العلوم الطبيعيه

The study was conducted in a greenhouse at the department of Horticulture and Landscape Gardening, College of Agriculture, University of Anbar for the growing season 2019-2020 and 2020-2021 to study the effect type of the growth media and spraying with nano and normal zinc and iron on mineral content of Strawberry leaves. First factor (M) included three of agricultural media: (River soil and Peat moss 3:1), (River soil and "50% Coantail + 50% Alfalfa" 3:1) and (River soil and poultry waste 3:1). The second factor (S) consisted of spraying with zinc and iron nano and normal with the following concentrations …

The Antioxidant And Antibacterial Activity Of Oriental Strawberry (Arbutus Andrachne L.): A Review Article, Sihem Mekchiche, Khalid Al-Ismail, Hamed Takruri

Hebron University Research Journal-A (Natural Sciences) - (مجلة جامعة الخليل للبحوث- أ (العلوم الطبيعيه

Oxidation is one of the most critical processes in food systems. It has an impact on a variety of dietary ingredient interactions, resulting in both desired and unwanted results. It was found in many studies that high concentrations of synthetic antioxidants may havesome carcinogenic effects in some animals, thus natural antioxidants would be analternative to synthetic antioxidants in food. These natural antioxidants can be present in any plant part, including grains, fruits, leaves, nuts, seeds, roots and barks. Many studies showed that there is an inverse relationship between the dietary intake of medicinal plants rich in antioxidants and the prevalence …

Inheritance Of 2,4‑Dichlorophenoxyacetic Acid (2,4‑D) Resistance In Amaranthus Palmeri, Chandrima Shyam, Dallas E. Peterson, Amit J. Jhala, Mithila Jugulam

Department of Agronomy and Horticulture: Faculty Publications

In this study, the inheritance of 2,4-D resistance in a multiple herbicide-resistant Palmer amaranth (KCTR) was investigated. Direct and reciprocal crosses were performed using 2,4-D-resistant KCTR and susceptible KSS plants to generate F₁ progenies. 2,4-D dose–response assays were conducted to evaluate the response of progenies from each F₁ family along with KCTR and KSS plants in controlled environmental growth chambers. Additionally, 2,4-D-resistant male and female plants from each of the F₁ families were used in pairwise crosses to generate pseudo-F₂ families. Segregation (resistance or susceptibility) of progenies from the F₂ families in response to a …

A Stealth Health Approach To Dietary Fibre, P. Stephen Baenziger, Katherine Anna Frels, Steve Greenspan, Julie Jones, Alison Lovegrove, Devin J. Rose, Peter Shewry, Rod Wallace

Department of Agronomy and Horticulture: Faculty Publications

Average dietary fibre intakes have increased little in the past twenty years in many countries, including the USA¹ . Multi-million-dollar campaigns promoting fruits, vegetables, whole grains and other foods high in fibre have delivered only small changes in diets² , and consumers have not changed from traditional staples to whole-grain options³ . UK millers report that consumption of whole-wheat bread has actually declined over the past decade (P. Shewry, personal communication). In the US, white flour, which is lower in fibre than whole-wheat flour, accounts for nearly 40% of the fibre intake⁴ . We believe that …

Antioxidant And Α-Amylase Inhibitory Activities And Starch Hydrolysis Kinetics Of Kidney Bean By Simulating Digestion In Vitro, Shi Juan, Bao Hong-Hui, Zhang Pei, Zhang Ti-Pei, Zhou Rui

Food and Machinery

Objective: To evaluate the physiological effects of whole kidney been food and develop functional resources of different color kidney beans. Methods: The contents of total polyphenols, flavonoids and acid polysaccharides, and antioxidant and α-amylase inhibitory activities of digestive solution and starch hydrolysis kinetics and glucose diffusion properties of three kidney bean were investigated through in vitro simulated gastrointestinal digestion. Results: The contents of total polyphenols, flavonoids and acid polysaccharides, and antioxidant and α-amylase inhibitory activities were black kidney bean＞red kidney bean＞white kidney bean (P＜0.05). The kinetic rate of first order reaction of starch hydrolysis, rapidly digestible starch …

Research Progress On Extraction Of Phenols With Natural Deep Eutectic Solvent, Zhang Yi-Xin, Wu Xu-Ran, He Ruo-Han, Feng Shuo, Yang Feng-Qi

Food and Machinery

This review summarized the research status of NADES on the extraction and separation of phenols, discussed the main factors affecting the extraction efficiency, and prospected the future development direction.

Research Progress On Properties, Industrial Production Of Reduced Glutathione And Its Application In Big Health Industry, Cai You-Hua, Wu Zi-Hao, Huang Xiao-Chen, Lin Yu-Kuan, Zheng Ming-Ying

Food and Machinery

The structural characteristics and physiological activities of reduced glutathione (GSH), as well as its metabolic pathways in vivo were reviewed, and the research progress in preparation, extraction, purification, application and industrial production of GSH were summarized. Moreover, the future research directions in functional activity, industrial production and product application of GSH were prospected.

Study On Nutrition Labeling Optimization Based On Augmented Reality, Huang Ze-Ying, Li Hai-Jun

Food and Machinery

To optimize nutrition labeling in China, this paper tried to overlay virtual information on original information from the nutrition information table by using augmented reality (AR), and then added three new guidance functions of nutrition labeling knowledge popularization, nutritional content auxiliary calculation and precise nutrition guidance through database construction, 3D modeling building and mobile phone application making. For the implementation of AR nutrition labeling, some suggestions were put forward, such as the establishment of national nutrition health database, the forecast of the implementation effect of the labeling and the operation mechanism design.

Analysis And Comprehensive Evaluation Of Amino Acid Composition Of Coreopsis Tinctoria From Different Habitats, Lu Qing-Yao, Zhou Min, Zhou Hui-Ling, Jiao Shi-Rong

Food and Machinery

Objective: This study focuses on in-depth research and development of the utilization of Coreopsis tinctoria resources. Methods: The Coreopsis samples from Hetian, Urumqi, Aba, Luding and Xining were hydrolyzed with hydrochloric acid, and the contents of 17 kinds of amino acids were determined by automatic amino acid analyzer. On this basis, the characteristics of amino acid flavor were analyzed and compared with the amino acid pattern spectrum issued by FAO/WHO. RAA, RC and SRC were calculated, and correlation analysis and cluster analysis were performed on 17 kinds of amino acids of chrysanthemum. Thereafter, the nutritional value of Coreopsis …

Correlation Analysis Of Taste Intensity Of Tea Flavor Attribute Reference Based On Electronic Tongue And Artificial Senses, Liu Zhong-Ying, Ran Qian-Song, Zhang Tuo, Zheng Wen-Jia, Pan Ke

Food and Machinery

Objective: This study aimed to explore the correlation between electronic tongue and artificial senses on the taste intensity of tea taste attribute reference. Methods: Quinine, alum, sodium glutamate, sucrose and citric acid were used as the reference materials for bitter, astringent, fresh, sweet and sour taste attributes in turn. Based on the perception threshold, the relationship between concentration and taste intensity of each reference material for electronic tongue and artificial senses and its correlation were analyzed. Results: The bitterness perception threshold of quinine was 0.015 mg/mL, and the corresponding electronic tongue response value was 4.91. The detection …

Flavor Analysis Of Passion Beer Based On Gc-Ims Technology, Gong Xiao, Chen Ting-Hui, Hu Xiao-Jun, Fan Wei-Wei, Li Ya-Jun, Zhao Xin-Qiang

Food and Machinery

Objective: This study aimed to fully develop and utilize the resources of passion fruits. Methods: The volatile flavor compounds at different brewing stages were analyzed by GC-IMS technology. Results: A total of 63 kinds of flavor compounds were identified, including 26 kinds of esters, 10 kinds of alcohols, 9 kinds of aldehydes, 7 kinds of ketones, 6 kinds of alkanes, 2 kinds of acids, 2 kinds of furans and 1 kinds of ether. Principal components analysis (PCA) showed that the cumulative difference contribution rate of the first two principal components (PC1 and PC2) was 81.2%, indicating that …

Determination Of The Residues Of 15 Kinds Of Benzodiazepine Sedatives In Aquatic Products By Liquid Chromatography Tandem Mass Spectrometry, Zhang Qiu-Yun, Yang Hong-Sheng, Tan Xiu-Hui, Zhu Xiao-Hua, Chen Feng-Wei, Chen Hui-Min

Food and Machinery

Objective: This study aimed to eslablish a method for the determination of 15 benzodiazepine sedatives in aquatic products by liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Methods: The sample was extracted with acetonitrile solvent, and the extract was purified by solid phase extraction (Oasis PRIME HLB) column. After concentration, the mobile phase was used to determine the volume. The mobile phase consisted of 1% formic acid aqueous solution and acetonitrile solvent. The method was performed in multiple reaction monitoring (MRM) mode and quantified by internal standard method. Results: The results showed that the linear range of 15 sedatives …