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Department of Agronomy and Horticulture: Faculty Publications

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Full-Text Articles in Agriculture

Introduction To Hydroponics- 2025-2026 Workshop Handbook, Stacy A. Adams Jan 2025

Introduction To Hydroponics- 2025-2026 Workshop Handbook, Stacy A. Adams

Department of Agronomy and Horticulture: Faculty Publications

Hydroponically grown plants grow faster than traditional production systems, with greater productivity, greater palatability, and having optimal visual character. Three principal components of a system include the plant support platform, a nutrient solution reservoir, and an interface for the root-solution. This publication provides in-depth explanation to system design, evaluation of water quality, nutrient formulation, and prototype systems that can be easily constructed for those wanting to explore hydroponics, such as educators, organizational leaders, or home enthusiasts.


Terrarium- An Ecosystem In A Jar, Stacy A. Adams Jan 2025

Terrarium- An Ecosystem In A Jar, Stacy A. Adams

Department of Agronomy and Horticulture: Faculty Publications

STEM education is supported through hand's-on activities in the classroom. Content understanding and reaffirmation of knowledge is supported through this informative STEM-Quick card that teachers or extension educators can distribute to participants for reference. Terrariums provide an example of a closed ecosystem where water and carbon cycling is demonstrated and plant growth can be observed. This card presents the steps to building and caring for an easy to create terrarium.


Greenhouse Management For The Smaller Structure--Educational And Hobby, Stacy A. Adams Jan 2025

Greenhouse Management For The Smaller Structure--Educational And Hobby, Stacy A. Adams

Department of Agronomy and Horticulture: Faculty Publications

Greenhouses used for teaching or by hobbyist are smaller than those used for commercial production or found in the retail environment. Structural materials, covering, mechanical equipment, and control systems often are packaged as a kit made available through greenhouse suppliers. Supplier construction services may not be available, in which buyers must use local contractors to complete the installation. Greenhouses are unique structures that differ greatly from sheds or other outbuildings. They have coverings that allow maximum light transmittance, protected from UV degradation, and can handle wide inside to outside temperature extremes. This extension circular provides valuable information on the character, …


Broad-Spectrum Resistance To Fungal Foliar Diseases In Wheat: Recent Efforts And Achievements, Amira M.I. Mourad, Asmaa A.M. Ahmed, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam Nov 2024

Broad-Spectrum Resistance To Fungal Foliar Diseases In Wheat: Recent Efforts And Achievements, Amira M.I. Mourad, Asmaa A.M. Ahmed, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam

Department of Agronomy and Horticulture: Faculty Publications

Wheat (Triticum spp.) is one of the most important cereal crops in the world. Several diseases affect wheat production and can cause 20-80% yield loss annually. Out of these diseases, stripe rust, also known as yellow rust (Puccinia striiformis f. sp. tritici), stem rust (Puccinia graminis f. sp. tritici), leaf rust (Puccinia recondita), and powdery mildew (Blumeria graminis f. sp. tritici) are the most important fungal diseases that infect the foliar part of the plant. Many efforts were made to improve wheat resistance to these diseases. Due to the continuous advancement in …


Disentangling Genotype And Environment Specific Latent Features For Improved Trait Prediction Using A Compositional Autoencoder, Anirudha Powadi, Talukder Zaki Jubery, Michael C. Tross, James C. Schnable, Baskar Ganapathysubramanian Nov 2024

Disentangling Genotype And Environment Specific Latent Features For Improved Trait Prediction Using A Compositional Autoencoder, Anirudha Powadi, Talukder Zaki Jubery, Michael C. Tross, James C. Schnable, Baskar Ganapathysubramanian

Department of Agronomy and Horticulture: Faculty Publications

In plant breeding and genetics, predictive models traditionally rely on compact representations of high-dimensional data, often using methods like Principal Component Analysis (PCA) and, more recently, Autoencoders (AE). However, these methods do not separate genotype-specific and environment-specific features, limiting their ability to accurately predict traits influenced by both genetic and environmental factors. We hypothesize that disentangling these representations into genotype-specific and environment-specific components can enhance predictive models. To test this, we developed a compositional autoencoder (CAE) that decomposes high-dimensional data into distinct genotype-specific and environment-specific latent features. Our CAE framework employed a hierarchical architecture within an autoencoder to effectively separate …


Industrial Hemp Biomass Negatively Affected By Herbicide Drift From Corn And Soybean Herbicides, Milos Zaric, Bruno Canella Vieira, Barbara Houston, Guilherme Sousa Alves, Samuel E. Wortman, Julie A. Peterson, Greg R. Kruger Nov 2024

Industrial Hemp Biomass Negatively Affected By Herbicide Drift From Corn And Soybean Herbicides, Milos Zaric, Bruno Canella Vieira, Barbara Houston, Guilherme Sousa Alves, Samuel E. Wortman, Julie A. Peterson, Greg R. Kruger

Department of Agronomy and Horticulture: Faculty Publications

The establishment of industrial hemp (Cannabis sativa L.) fields near row crops has raised concerns about the potential adverse effects of herbicide drift on hemp production. This study examined hemp susceptibility to drift of herbicides registered for use in corn and/or soybeans. Herbicide solutions (2,4-D, dicamba, glufosinate, glyphosate, imazethapyr, lactofen, mesotrione) were applied separately in the wind tunnel (3.6 m s−1 airspeed), simulating drift scenarios, with conventional TP95015EVS (TP) and air inclusion AI95015EVS (AI) flat fan nozzles calibrated to deliver 140 L ha−1 carrier volume at 230 kPa. Mylar cards and hemp plants (20–25 cm tall) were placed …


Twas Facilitates Gene-Scale Trait Genetic Dissection Through Gene Expression, Structural Variations, And Alternative Splicing In Soybean, Delin Li, Qi Wang, Yu Tian, Xiangguang Lyv, Hao Zhang, Huilong Hong, Huawei Gao, Yan-Fei Li, Chaosen Zhao, Jiajun Wang, Ruizhen Wang, Jinliang Yang, Bin Liu, Patrick S. Schnable, James C. Schnable, Ying-Hui Li, Li-Juan Qiu Oct 2024

Twas Facilitates Gene-Scale Trait Genetic Dissection Through Gene Expression, Structural Variations, And Alternative Splicing In Soybean, Delin Li, Qi Wang, Yu Tian, Xiangguang Lyv, Hao Zhang, Huilong Hong, Huawei Gao, Yan-Fei Li, Chaosen Zhao, Jiajun Wang, Ruizhen Wang, Jinliang Yang, Bin Liu, Patrick S. Schnable, James C. Schnable, Ying-Hui Li, Li-Juan Qiu

Department of Agronomy and Horticulture: Faculty Publications

A genome-wide association study (GWAS) identifies trait-associated loci, but identifying the causal genes can be a bottleneck, due in part to slow decay of linkage disequilibrium (LD). A transcriptome-wide association study (TWAS) addresses this issue by identifying gene expression–phenotype associations or integrating gene expression quantitative trait loci with GWAS results. Here, we used self-pollinated soybean (Glycine max [L.] Merr.) as a model to evaluate the application of TWAS to the genetic dissection of traits in plant species with slow LD decay. We generated RNA sequencing data for a soybean diversity panel and identified the genetic expression regulation of 29 …


Dissecting The Genetic Architecture Of Sunflower Disc Diameter Using Genome-Wide Association Study, Yavuz Delen, Ravi V. Mural, Semra Palali-Delen, Gen Xu, James C. Schnable, Ismail M. Dweikat, Jinliang Yang Sep 2024

Dissecting The Genetic Architecture Of Sunflower Disc Diameter Using Genome-Wide Association Study, Yavuz Delen, Ravi V. Mural, Semra Palali-Delen, Gen Xu, James C. Schnable, Ismail M. Dweikat, Jinliang Yang

Department of Agronomy and Horticulture: Faculty Publications

Sunflower (Helianthus annuus L.) plays an essential role in meeting the demand for edible oil worldwide. The yield of sunflower seeds encompasses several component traits, including the disc diameter. Over three consecutive years, 2019, 2020, and 2022, we assessed phenotypic variation in disc diameter across a diverse set of sunflower accessions (N = 342) in replicated field trials. Upon aggregating the phenotypic data from multiple years, we estimated the broad sense heritability (H2) of the disc diameter trait to be 0.88. A subset of N = 274 accessions was genotyped by using the tunable genotyping-by-sequencing (tGBS) method, …


Association Mapping Of Important Agronomic Traits In Mucuna Pruriens (L.) Dc., Patrush Lepcha, Mahesh Shekhar, Leelambika Murugesan, Mahammad Jaheer, Ratan Chopra, Vikas Belamkar, Narayana Sathyanarayana Aug 2024

Association Mapping Of Important Agronomic Traits In Mucuna Pruriens (L.) Dc., Patrush Lepcha, Mahesh Shekhar, Leelambika Murugesan, Mahammad Jaheer, Ratan Chopra, Vikas Belamkar, Narayana Sathyanarayana

Department of Agronomy and Horticulture: Faculty Publications

Background The tropical legume Mucuna pruriens (L.) DC. can meet three agricultural needs: low-cost protein, highvalue medicines, and green manure or cover crops. But like other underutilized crops, it needs more modern breeding resources. Identifying marker-trait associations (MTAs) can facilitate marker-assisted breeding and crop improvement. Recent studies have demonstrated the feasibility of identifying MTAs using a small number of accessions (< 100). We have characterized a panel of 70 M. pruriens accessions across two consecutive years and performed association analysis for 16 phenotypic traits related to seed (seed length, seed width, seed thickness, seed yield per plant, hundred seed weight); pod (pod length, pod width, number of pods …


Greater Aperture Counteracts Effects Of Reduced Stomatal Density On Water Use Efficiency: A Case Study On Sugarcane And Meta-Analysis, Daniel Lunn, Baskaran Kannan, Amandine Germon, Alistair Leverett, Thomas E. Clemente, Fredy Altpeter, Andrew D.B. Leakey Jul 2024

Greater Aperture Counteracts Effects Of Reduced Stomatal Density On Water Use Efficiency: A Case Study On Sugarcane And Meta-Analysis, Daniel Lunn, Baskaran Kannan, Amandine Germon, Alistair Leverett, Thomas E. Clemente, Fredy Altpeter, Andrew D.B. Leakey

Department of Agronomy and Horticulture: Faculty Publications

Stomata regulate CO2 and water vapor exchange between leaves and the atmosphere. Stomata are a target for engineering to improve crop intrinsic water use efficiency (iWUE). One example is by expressing genes that lower stomatal density (SD) and reduce stomatal conductance (gsw). However, the quantitative relationship between reduced SD, gsw, and the mechanisms underlying it is poorly understood. We addressed this knowledge gap using low-SD sugarcane (Saccharum spp. hybrid) as a case study alongside a meta-analysis of data from 10 species. Transgenic expression of EPIDERMAL PATTERNING FACTOR 2 from Sorghum bicolor (SbEPF2) in sugarcane …


Revisiting Fr13a For Submergence Tolerance: Beyond The Sub1a Gene, Waseem Hussain, Mahender Anumalla, Abdelbagi M. Ismail, Harkamal Walia, Vistas Kumar Singh, Ajay Kohli, Sankalp Bhosale, Hans Bhardwaj Jul 2024

Revisiting Fr13a For Submergence Tolerance: Beyond The Sub1a Gene, Waseem Hussain, Mahender Anumalla, Abdelbagi M. Ismail, Harkamal Walia, Vistas Kumar Singh, Ajay Kohli, Sankalp Bhosale, Hans Bhardwaj

Department of Agronomy and Horticulture: Faculty Publications

Rice landrace FR13A is the original donor of the SUB1A gene. FR13A harbors hidden genetic variation that surpasses the submergence tolerance level of SUB1A. Here, we provide an overview of the unique features of FR13A associated with exceptional recovery ability that have been overlooked since the significant discovery of the SUB1 locus. We present a detailed overview of how to underpin the new genes associated with the recovery ability seen in FR13A. Finally, we propose a unique approach to develop rice cultivars that surpass submergence tolerance beyond the SUB1 locus from FR13A.


Tissue‑ And Time‑Dependent Metabolite Profiles During Early Grain Development Under Normal And High Night‑Time Temperature Conditions, Nathan Abshire, Andrew L. Hauck, Harkamal Walia, Toshihiro Obata Jun 2024

Tissue‑ And Time‑Dependent Metabolite Profiles During Early Grain Development Under Normal And High Night‑Time Temperature Conditions, Nathan Abshire, Andrew L. Hauck, Harkamal Walia, Toshihiro Obata

Department of Agronomy and Horticulture: Faculty Publications

Background Wheat grain development in the first few days after pollination determines the number of endosperm cells that influence grain yield potential and is susceptible to various environmental conditions, including high night temperatures (HNTs). Flag leaves and seed-associated bracts (glumes, awn, palea, and lemma) provide nutrients to the developing seed. However, the specific metabolic roles of these tissues are uncertain, especially their dynamics at different developmental stages and the time in a day. Tissue- and time-dependent metabolite profiling may hint at the metabolic roles of tissues and the mechanisms of how HNTs affect daytime metabolic status in early grain development. …


Adopting Yield-Improving Practices To Meet Maize Demand In Sub-Saharan Africa Without Cropland Expansion, Fernando Aramburu-Merlos, Fatima A. M. Tenorio, Nester Mashingaidze, Alex Sananka, Stephen Aston, Jonathan J. Ojeda, Patricio Grassini May 2024

Adopting Yield-Improving Practices To Meet Maize Demand In Sub-Saharan Africa Without Cropland Expansion, Fernando Aramburu-Merlos, Fatima A. M. Tenorio, Nester Mashingaidze, Alex Sananka, Stephen Aston, Jonathan J. Ojeda, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Maize demand in Sub-Saharan Africa is expected to increase 2.3 times during the next 30 years driven by demographic and dietary changes. Over the past two decades, the area croppedwithmaize has expanded by 17million hectares in the region, with limited yield increase. Following this trend could potentially result in further maize cropland expansion and the need for imports to satisfy domestic demand. Here, we use data collected from 14,773 smallholder fields in the region to identify agronomic practices that can improve farm yield gains. We find that agronomic practices related to cultivar selection, and nutrient, pest, and crop management can …


Evaluating The Yield Of Surviving Plants From Early-Season Hail Damage In Corn: A Field Survey, I. P. Lisboa, Christopher A. Proctor, R W. Elmore, A. J. Mcmechan, N. D. Mueller, J. Wilson, G. Zobeck, Aaron Nygren, A. Bastidas, Osler Ortez May 2024

Evaluating The Yield Of Surviving Plants From Early-Season Hail Damage In Corn: A Field Survey, I. P. Lisboa, Christopher A. Proctor, R W. Elmore, A. J. Mcmechan, N. D. Mueller, J. Wilson, G. Zobeck, Aaron Nygren, A. Bastidas, Osler Ortez

Department of Agronomy and Horticulture: Faculty Publications

Economic losses due to hailstorms acrossUS corn fields occur every year.Hailstorms result in leaf defoliation, decreasing photosynthetic area and impairing carbon assimilation and crop yield for all corn (Zea mays L.) development stages. However, more attention is often given to stand reductions rather than damage to stems or leaf defoliation. During the 2014 growing season, a natural hail event affected many areas of eastern Nebraska. Twelve affected corn fields were surveyed and included in this study. An injury score scale (1–5) was developed based on various injury and severity levels before the V6 (6-leaf corn growth stage) (six-collared leaves). …


Natural Variation In Lonely Guy-Like 1 Regulates Rice Grain Weight Under Warmer Night Conditions, Jaspreet Sandhu, Larissa Irvin, Anil Kumar Chandaran, Shohei Oguro, Puneet Paul, Balpreet Dhatt, Waseem Hussain, Shannon S. Cunningham, Cherryl O. Quinones, Argelia Lorence, Maria Arlene Adviento-Borbe, Paul E. Staswick, Gota Morota, Harkamal Walia May 2024

Natural Variation In Lonely Guy-Like 1 Regulates Rice Grain Weight Under Warmer Night Conditions, Jaspreet Sandhu, Larissa Irvin, Anil Kumar Chandaran, Shohei Oguro, Puneet Paul, Balpreet Dhatt, Waseem Hussain, Shannon S. Cunningham, Cherryl O. Quinones, Argelia Lorence, Maria Arlene Adviento-Borbe, Paul E. Staswick, Gota Morota, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Global nighttime temperatures are rising at twice the rate of daytime temperatures and pose a challenge for rice (Oryza sativa) production. High nighttime temperature (HNT) stress affects rice yield by reducing grain weight, size, and fertility. Although the genes associated with these yield parameters have been identified and characterized under normal temperatures, the genetic basis of grain weight regulation under HNT stress remains less explored. We examined the natural variation for rice single grain weight (SGW) under HNT stress imposed during grain development. A genome-wide association analysis identified several loci associated with grain weight under HNT stress. A …


Overexpression Of Msdreb1c Modulates Growth And Improves Forage Quality In Tetraploid Alfalfa (Medicago Sativa L.), Yangyang Zhang, Zhen Wang, Fan Zhang, Xue Wang, Yajing Li, Ruicai Long, Mingna Li, Xianyang Li, Quanzhen Wang, Qingchuan Yang, Junmei Kang Apr 2024

Overexpression Of Msdreb1c Modulates Growth And Improves Forage Quality In Tetraploid Alfalfa (Medicago Sativa L.), Yangyang Zhang, Zhen Wang, Fan Zhang, Xue Wang, Yajing Li, Ruicai Long, Mingna Li, Xianyang Li, Quanzhen Wang, Qingchuan Yang, Junmei Kang

Department of Agronomy and Horticulture: Faculty Publications

DREB has been reported to be involved in plant growth and response to environmental factors. However, the function of DREB in growth and development has not been elucidated in alfalfa (Medicago sativa L.), a perennial tetraploid forage cultivated worldwide. In this study, an ortholog of MtDREB1C was characterized from alfalfa and named MsDREB1C accordingly. MsDREB1C was significantly induced by abiotic stress. The transcription factor MsDREB1C resided in the nucleus and had self-transactivation activity. The MsDREB1C overexpression (OE) alfalfa displayed growth retardation under both long-day and short-day conditions, which was supported by decreased MsGA20ox and upregulated MsGA2ox in the OE …


Evaluating Consistency Across Multiple Neospectra (Compact Fourier Transform Near-Infrared) Spectrometers For Estimating Common Soil Properties, Sadia M. Mitu, Colleen Smith, Jonathan Sanderman, Richard R. Ferguson, Keith Shepherd, Yufeng Ge Apr 2024

Evaluating Consistency Across Multiple Neospectra (Compact Fourier Transform Near-Infrared) Spectrometers For Estimating Common Soil Properties, Sadia M. Mitu, Colleen Smith, Jonathan Sanderman, Richard R. Ferguson, Keith Shepherd, Yufeng Ge

Department of Agronomy and Horticulture: Faculty Publications

Rapid and cost-effective techniques for soil analysis are essential to guide sustainable land management and production agriculture. This study aimed at evaluating the performance and consistency of portable handheld Fourier-transform near-infrared spectrometers and the NeoSpectra scanners in estimating 12 common soil physical and chemical properties including pH; organic carbon (OC); inorganic carbon (IC); total nitrogen (TN); cation exchange capacity (CEC); clay, silt, and sand fractions; and exchangeable potassium (K), phosphorus (P), calcium (Ca), and magnesium (Mg). A diverse set of samples (n = 600) were retrieved from a national-scale soil archive of the Kellogg Soil Survey Laboratory of USDA-NRCS …


Accurate Measurement Of Field Size Is Essential For Analysis Of Smallholder Survey Data, Rica Joy Flor, Shen Yuan, Gonzalo Rizzo, Fahmuddin Agus, Anny Ruth P. Pame, Akhara Ouk, Rathmuny Then, Sokheng Keo, Virender Kumar, Particio Grassini Apr 2024

Accurate Measurement Of Field Size Is Essential For Analysis Of Smallholder Survey Data, Rica Joy Flor, Shen Yuan, Gonzalo Rizzo, Fahmuddin Agus, Anny Ruth P. Pame, Akhara Ouk, Rathmuny Then, Sokheng Keo, Virender Kumar, Particio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Context: Agronomic surveys play a vital role in identifying on-farm yield constraints and opportunities to increase productivity, input-use efficiency, and farmer profit. These surveys typically rely on farmer-reported data, where crop yield and applied inputs per hectare are estimated based on farmer-reported total harvested output and applied inputs per field and associated field size. At question is how accurate field size is, which is vital in the case of smallholders as small inaccuracies can lead to large biases in estimated yield and inputs.

Objectives: The goals of this study are to assess current approaches to determine field size …


Phenotypic Diversity For Morpho-Agronomic Traits In The Us Proso Millet (Panicum Miliaceum L.) Core Collection, Santosh G. Rajput, Rituraj Khound, Dipak K. Santra Apr 2024

Phenotypic Diversity For Morpho-Agronomic Traits In The Us Proso Millet (Panicum Miliaceum L.) Core Collection, Santosh G. Rajput, Rituraj Khound, Dipak K. Santra

Department of Agronomy and Horticulture: Faculty Publications

Proso millet (Panicum miliaceum L.) is a short-season annual crop known for high water-use efficiency and drought tolerance. The low water requirement makes this ancient grain an excellent rotational crop for the winter wheat-based dryland cropping system in theHigh Plains of theUnited States. The genetic base of the commonly grown US cultivars is very narrow. Assessment of proso millet germplasm for agronomic traits is essential for its efficient utilization in the genetic improvement of this crop. The objectives of this study were to (1) characterize the US proso millet germplasm based on nine important morpho-agronomic traits and (2) classify …


Soil Health Cycle, Bijesh Maharjan, Saurav Das, Vesh R. Thapa, Bharat Sharma Acharya Apr 2024

Soil Health Cycle, Bijesh Maharjan, Saurav Das, Vesh R. Thapa, Bharat Sharma Acharya

Department of Agronomy and Horticulture: Faculty Publications

Soil health is pivotal to agricultural sustainability. Promoting and sustaining soil health management is challenging since it involves many interdependent components and steps and is an iterative process. Herein, the soil health cycle (SHC) is proposed as a soil health management cycle encompassing human dimensions, management practices, and their effects on soil health indicators (SHIs), leading to subsequent impacts on soil functions. The SHC provides a structure for an iterative testing of changes to improve soil health. A systematic review of research publications was also conducted using the Web of Science database supplemented by Elicit AI and Scopus API searches …


Long-Term Soil Change In The Us Great Plains: An Evaluation Of The Haas Soil Archive, Mark A. Liebig, Francisco J. Calderon, Andrea K. Clemensen, Lisa Durso, Jessica L. Duttenhefner, Jed O. Eberly, Jonathan J. Halvorson, Virginia L. Jin, Kyle Mankin, Andrew J. Margenot, Catherine E. Stewart, Scott Van Pelt, Merle F. Vigil Mar 2024

Long-Term Soil Change In The Us Great Plains: An Evaluation Of The Haas Soil Archive, Mark A. Liebig, Francisco J. Calderon, Andrea K. Clemensen, Lisa Durso, Jessica L. Duttenhefner, Jed O. Eberly, Jonathan J. Halvorson, Virginia L. Jin, Kyle Mankin, Andrew J. Margenot, Catherine E. Stewart, Scott Van Pelt, Merle F. Vigil

Department of Agronomy and Horticulture: Faculty Publications

Diverse patterns of climate and edaphic factors challenge detection of soil property change in the US Great Plains. Because detectable soil change can take decades, insights into the trajectory of soil properties frequently require long-term site monitoring and, where available, associated soil archives to enable comparisons with initial or baseline states. Unfortunately, few multi-decadal soil change investigations have been conducted in this region. Here, we document effects of dryland cropping on a suite of soil properties by comparing matched historic (1947) and contemporary (2018) soil samples from the Haas Soil Archive at three sites in the US Great Plains: Moccasin, …


Imitating The “Breeder’S Eye”: Predicting Grain Yield From Measurements Of Non-Yield Traits, Hongyu Jin, Michael C. Tross, Ruijuan Tan, Linsey Newton, Ravi V. Mural, Jinliang Yang, Addie M. Thompson, James C. Schnable Mar 2024

Imitating The “Breeder’S Eye”: Predicting Grain Yield From Measurements Of Non-Yield Traits, Hongyu Jin, Michael C. Tross, Ruijuan Tan, Linsey Newton, Ravi V. Mural, Jinliang Yang, Addie M. Thompson, James C. Schnable

Department of Agronomy and Horticulture: Faculty Publications

Plant breeding relies on information gathered from field trials to select promising new crop varieties for release to farmers and to develop genomic prediction models that can enhance the efficiency of genetic improvement in future breeding cycles. However, generating the genetic marker data required to apply genomic prediction at the early stages of a breeding program remains costly for many public-sector breeding programs as well as for many plant breeders operating in developing countries. As the pace of climate change intensifies, the time lag of developing and deploying new crop varieties requires plant breeders to make selection decisions without knowing …


Pollen-Mediated Gene Flow From Herbicide-Resistant Yellow Corn To Non-Genetically Engineered Food-Grade White Corn, Mandeep Singh, Vipan Kumar, Stevan Z. Knezevic, John L. Lindquist, Suat Irmak, Santosh Pitla, Amit J. Jhala Mar 2024

Pollen-Mediated Gene Flow From Herbicide-Resistant Yellow Corn To Non-Genetically Engineered Food-Grade White Corn, Mandeep Singh, Vipan Kumar, Stevan Z. Knezevic, John L. Lindquist, Suat Irmak, Santosh Pitla, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Nebraska is the number one producer of food-grade white corn in the United States. Pollen-mediated gene flow (PMGF) from genetically engineered high alpha-amylase corn, known as Enogen corn, to food-grade white corn can have undesirable outcomes. Alpha-amylase can convert starch in white corn to sugar during or after its processing, degrading the quality of processed products. Thus, proximity to Enogen corn puts white corn production at risk. The objectives of this study were to evaluate the PMGF from herbicide-resistant yellow corn to food-grade white corn and assess the significance of wind speed and direction. Field experiments were set up using …


Leveraging Digital Agriculture For On-Farm Testing Of Technologies, Laila A. Puntel, Laura J. Thompson, Taro Mieno Mar 2024

Leveraging Digital Agriculture For On-Farm Testing Of Technologies, Laila A. Puntel, Laura J. Thompson, Taro Mieno

Department of Agronomy and Horticulture: Faculty Publications

The Precision Nitrogen Project (PNP) worked with more than 80 corn and winter wheat producers to inexpensively design and implement randomized, replicated field strip trials on whole commercial farm fields, and to provide site-specific testing of current nitrogen (N) technologies. This article proposes a conceptual framework and detailed procedure to select the N technology to be tested; design and implement field trials; generate, process, and manage field trial data; and automatically analyze, report, and share benefits from precision N technology. The selection of the N technology was farmer-driven to ensure a good fit and to increase the likelihood of future …


Variation In Leaf Chlorophyll Concentration In Response To Nitrogen Application Across Maize Hybrids In Contrasting Environments, Kyle M. Linders, Dipak K. Santra, James C. Schnable, Brandi Sigmon Mar 2024

Variation In Leaf Chlorophyll Concentration In Response To Nitrogen Application Across Maize Hybrids In Contrasting Environments, Kyle M. Linders, Dipak K. Santra, James C. Schnable, Brandi Sigmon

Department of Agronomy and Horticulture: Faculty Publications

Leaf chlorophyll concentration was measured for 84 publicly available maize hybrids grown under three nitrogen fertilizer treatments in two contrasting environments in Nebraska. The effect of nitrogen treatment on chlorophyll response was found to be significant (p < 0.05) for both locations. In Scottsbluff, chlorophyll concentrations increased significantly with increasing nitrogen rate, while no significant difference was found between medium and high nitrogen in Lincoln. Within equivalent nitrogen treatments, chlorophyll was more abundant in Lincoln than Scottsbluff for nearly every hybrid. Hybrid response was not consistent between environments, with approximately 11% of variance explained by genotype by environment interaction.


Calibration Of Hybrid-Maize Model For Simulation Of Soil Moisture And Yield In Production Corn Fields, Anthony A. Amori, Olufemi P. Abimbola, Trenton E. Franz, Daran Rudnick, Javed Iqbal, Haishun Yang Feb 2024

Calibration Of Hybrid-Maize Model For Simulation Of Soil Moisture And Yield In Production Corn Fields, Anthony A. Amori, Olufemi P. Abimbola, Trenton E. Franz, Daran Rudnick, Javed Iqbal, Haishun Yang

Department of Agronomy and Horticulture: Faculty Publications

Model calibration is essential for acceptable model performance and applications. The Hybrid-Maize model, developed at the University of Nebraska-Lincoln, is a process-based crop simulation model that simulates maize growth as a function of crop and field management and environmental conditions. In this study, we calibrated and validated the Hybrid-Maize model using soil moisture and yield data from eight commercial production fields in two years. We used a new method for the calibration and multi-parameter optimization (MPO) based on kriging with modified criteria for selecting the parameter combinations. The soil moisture-related parameter combination (SM-PC3) improved simulations of soil water dynamics, but …


U.S. Cereal Rye Winter Cover Crop Growth Database, Alexandra M. Huddell, Resham Thapa, Guillermo S. Marcillo, Lori J. Abendroth, Victoria J. Ackroyd, Shalamar D. Armstrong, Gautam Asmita, Muthukumar V. Bagavathiannan, Kipling S. Balkcom, Andrea Basche, Shawn Beam, Kevin Bradley, Lucas Pecci Canisares, Heather Darby, Adam S. Davis, Pratap Devkota, Warren A. Dick, Jeffery A. Evans, Wesley J. Everman, Tauana Ferreira De A Lmeida, Michael L. Flessner, Lisa M. Fultz, Stefan Gailans, Masoud Hashemi, Joseph Haymaker, Matthew J. Helmers, Nicholas Jordan, Thomas C. Kaspar, Quirine M. Ketterings, Eileen Kladivko, Alexandra Kravchenko, Eugene P. Law, Lauren Lazaro, Ramon G. Leon, Jeffrey Liebert, John Lindquist, Kristen Loria, Jodie M. Mcvane, Jarrod O. Miller, Michael J. Mulvaney, Nsalambi V. Nkongolo, Jason K. Norsworthy, Binaya Parajuli, Christopher Pelzer, Cara Peterson, Hanna Poffenbarger, Pratima Poudel, Mark S. Reiter, Matt Ruark, Matthew R. Ryan, Spencer Samuelson, John E. Sawyer, Sarah Seehaver, Lovreet S. Shergill, Yogendra Raj Upadhyaya, Mark Vangessel, Ashley L. Waggoner, John M. Wallace, Samantha Wells, Charles White, Bethany Wolters, Alex Woodley, Rongzhong Ye, Eric Youngerman, Brian A. Needelman, Steven B. Mirsky Feb 2024

U.S. Cereal Rye Winter Cover Crop Growth Database, Alexandra M. Huddell, Resham Thapa, Guillermo S. Marcillo, Lori J. Abendroth, Victoria J. Ackroyd, Shalamar D. Armstrong, Gautam Asmita, Muthukumar V. Bagavathiannan, Kipling S. Balkcom, Andrea Basche, Shawn Beam, Kevin Bradley, Lucas Pecci Canisares, Heather Darby, Adam S. Davis, Pratap Devkota, Warren A. Dick, Jeffery A. Evans, Wesley J. Everman, Tauana Ferreira De A Lmeida, Michael L. Flessner, Lisa M. Fultz, Stefan Gailans, Masoud Hashemi, Joseph Haymaker, Matthew J. Helmers, Nicholas Jordan, Thomas C. Kaspar, Quirine M. Ketterings, Eileen Kladivko, Alexandra Kravchenko, Eugene P. Law, Lauren Lazaro, Ramon G. Leon, Jeffrey Liebert, John Lindquist, Kristen Loria, Jodie M. Mcvane, Jarrod O. Miller, Michael J. Mulvaney, Nsalambi V. Nkongolo, Jason K. Norsworthy, Binaya Parajuli, Christopher Pelzer, Cara Peterson, Hanna Poffenbarger, Pratima Poudel, Mark S. Reiter, Matt Ruark, Matthew R. Ryan, Spencer Samuelson, John E. Sawyer, Sarah Seehaver, Lovreet S. Shergill, Yogendra Raj Upadhyaya, Mark Vangessel, Ashley L. Waggoner, John M. Wallace, Samantha Wells, Charles White, Bethany Wolters, Alex Woodley, Rongzhong Ye, Eric Youngerman, Brian A. Needelman, Steven B. Mirsky

Department of Agronomy and Horticulture: Faculty Publications

Winter cover crop performance metrics (i.e., vegetative biomass quantity and quality) affect ecosystem services provisions, but they vary widely due to differences in agronomic practices, soil properties, and climate. Cereal rye (Secale cereale) is the most common winter cover crop in the United States due to its winter hardiness, low seed cost, and high biomass production. We compiled data on cereal rye winter cover crop performance metrics, agronomic practices, and soil properties across the eastern half of the United States. The dataset includes a total of 5,695 cereal rye biomass observations across 208 site-years between 2001–2022 and encompasses …


Intensifying Rice Production To Reduce Imports And Land Conversion In Africa, Shen Yuan, Kazuki Saito, Pepijn A.J. Van Oort, Martin K. Van Ittersum, Shaobing Peng, Patricio Grassini Jan 2024

Intensifying Rice Production To Reduce Imports And Land Conversion In Africa, Shen Yuan, Kazuki Saito, Pepijn A.J. Van Oort, Martin K. Van Ittersum, Shaobing Peng, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Africa produces around 60% of the rice the continent consumes, relying heavily on rice imports to fulfill the rest of the domestic demand.Over the past 10 years, the rice-agricultural area increased nearly 40%, while average yield remained stagnant. Here we used a process-based crop simulation modelling approach combined with local weather, soil, and management datasets to evaluate the potential to increase rice production on existing cropland area in Africa and assess cropland expansion and rice imports by year 2050 for different scenarios of yield intensification. We find that Africa can avoid further increases in rice imports, and even reduce them, …


Filling The Agronomic Data Gap Through A Minimum Data Collection Approach, Fatima A. Tenorio, Juan I. Rattalino Edreira, Juan Pablo Monzon, Fernando Aramburu-Merlos, Achim Dobermann, Armelle Gruere, Juan Martin Brihet, Sofia Gayo, Shawn Conley, Spyridon Mourtzinis, Nester Mashingaidze, Alex Sananka, Stephen Aston, Jonathan J. Ojeda, Patricio Grassini Jan 2024

Filling The Agronomic Data Gap Through A Minimum Data Collection Approach, Fatima A. Tenorio, Juan I. Rattalino Edreira, Juan Pablo Monzon, Fernando Aramburu-Merlos, Achim Dobermann, Armelle Gruere, Juan Martin Brihet, Sofia Gayo, Shawn Conley, Spyridon Mourtzinis, Nester Mashingaidze, Alex Sananka, Stephen Aston, Jonathan J. Ojeda, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Context: Agronomic data such as applied inputs, management practices, and crop yields are needed for assessing productivity, nutrient balances, resource use efficiency, as well as other aspects of environmental and economic performance of cropping systems. In many instances, however, these data are only available at a coarse level of aggregation or simply do not exist.

Objectives: Here we developed an approach that identifies sites for agronomic data collection for a given crop and country, seeking a balance between minimizing data collection efforts and proper representation of the main crop producing areas.

Methods: The developed approach followed a …


High Target Homology Does Not Guarantee Inhibition: Aminothiazoles Emerge As Inhibitors Of Plasmodium Falciparum, Sandra Johannsen, Robin M. Gierse, Arne KrüGer, Rachel L. Edwards, Vittoria Nanna, Anna Fontana, Di Zhu, Tiziana Masini, Lais Pessanha De Carvalho, Mael Poizat, Bart Kieftenbelt, Dana M. Hodge, Sophie University Of Nebraska-Lincoln,, Daan Bunt, Antoine Lacour, Atanaz Shams, Kamila Anna Meissner, Edmarcia Elisa De Souza, Melloney Dröge, Bernard Van Vliet, Jack Den Hartog, Michael C. Hutter, Jana Held, Audrey R. Odom John, Carsten Wrenger, Anna K.H. Hirsch Jan 2024

High Target Homology Does Not Guarantee Inhibition: Aminothiazoles Emerge As Inhibitors Of Plasmodium Falciparum, Sandra Johannsen, Robin M. Gierse, Arne KrüGer, Rachel L. Edwards, Vittoria Nanna, Anna Fontana, Di Zhu, Tiziana Masini, Lais Pessanha De Carvalho, Mael Poizat, Bart Kieftenbelt, Dana M. Hodge, Sophie University Of Nebraska-Lincoln,, Daan Bunt, Antoine Lacour, Atanaz Shams, Kamila Anna Meissner, Edmarcia Elisa De Souza, Melloney Dröge, Bernard Van Vliet, Jack Den Hartog, Michael C. Hutter, Jana Held, Audrey R. Odom John, Carsten Wrenger, Anna K.H. Hirsch

Department of Agronomy and Horticulture: Faculty Publications

In this study, we identified three novel compound classes with potent activity against Plasmodium falciparum, the most dangerous human malarial parasite. Resistance of this pathogen to known drugs is increasing, and compounds with different modes of action are urgently needed. One promising drug target is the enzyme 1-deoxy-D-xylulose-5- phosphate synthase (DXPS) of the methylerythritol 4-phosphate (MEP) pathway for which we have previously identified three active compound classes against Mycobacterium tuberculosis. The close structural similarities of the active sites of the DXPS enzymes of P. falciparum and M. tuberculosis prompted investigation of their antiparasitic action, all classes …