Life Sciences Commons^™

Predicting The Cutting Time Of Cottage Cheese Using Backscatter Measurements, Czarena L. Crofcheck, Frederick A. Payne, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

An automated system for monitoring culture growth and determining coagulum cutting time is needed for cottage cheese manufacturing. A light backscatter measurement system was designed and installed in a local cottage cheese manufacturing plant. A cutting time prediction algorithm was developed using parameters generated from the backscatter profile. The cutting time prediction algorithm, T_cut = T_max + β₂ S, used two time-based parameters generated from the backscatter profile (T_max and S) and one operator selected parameter, β₂, to predict the coagulum cutting time, Tcut. The standard error of prediction for the algorithm was 6.4 …

Standardization Of An Immunoassay For The Detection Of Antibodies To B2 Glycoprotein-I, Eric N. Erickson Jr.

Seton Hall University Dissertations and Theses (ETDs)

Antiphospholipid Syndrome (APS) is an evolving autoimmune disease with numerous clinical manifestations. APS occurs in two forms: Primary Antiphospholipid Syndrome (PAPS) and secondarily in association with other autoimmune disorders such as Systemic Lupus Erythematosis (SLE). In the brief period since its discovery as a cofactor for anticardiolipin antibodies, p1-glycoprotein I (�GPI; apolipoprotein H) has been recognized as the autoantigen in the absence of anionic phospholipid when appropriately presented to human autoantibodies. Recent studies suggest that this presentation requires the surface-dependent unmasking of a cryptic epitope. Immunoassays of autoantibodies directed against p1GPI, developed through the application of suitable polymeric matrices, may …

Blue Crab Larval Dispersion And Retention In The Mississippi Bight, Donald R. Johnson, Harriet M. Perry

Faculty Publications

A conceptual hypothesis relating physical forcing to dispersion and retention was developed for blue crab larvae within the Mississippi Eight. The spawning period for blue crabs in the northern Gulf of Mexico is protracted. Hatching of eggs occurs near the barrier islands and mouths of coastal bays from March through October. Larvae are released on ebbing tides and spend the next 30 to 50 d offshore where they develop through seven zoeal stages before undergoing metamorphosis to megalopae. Duration of the megalopal stage is variable but generally persists from 6 to 20 d. Blue crabs recruit to Gulf estuaries as …

Age And Growth Of Cobia, Rachycentron Canadum, From The Northeastern Gulf Of Mexico, James S. Franks, James R. Warren, Michael V. Buchanan

Faculty Publications

We examined 1005 cobia, Rachycentron canadum, from recreational catches in the northeastern Gulf of Mexico from 1987 to 1995. Specimens ranged from 325 to 1651 mm fork length (FL); females had a mean FL of 1050 mm (n=730) and were significantly larger than males that had a mean FL of 952 mm (n=275). The over all male to female ratio was 1:2.7. Ages of 565 cobia were estimated from thin-sectioned otoliths (sagittae). Marginal-increment analysis of sagittal otoliths showed a single annual minimum during June. Male cobia (n=170; 525-1330 mm FL) ranged from age 0 to 9, and females (n=395; 493-1651 …

Identification And Characterization Of Determinants Of Head And Neck Tumor Cell Invasion, Yangguan Wu

Theses and Dissertations in Biomedical Sciences

Head and neck squamous cell carcinoma (HNSCC) is a common malignant disease with poor prognosis. The majority of patients die from local invasion or lymphatic metastasis. The mechanism(s) underlining the invasiveness of HNSCC are poorly understood. Utilizing a panel of HNSCC cell lines previously established in our laboratory, we tested the application and relevance of the three-step hypothesis of tumor invasion to HNSCC and investigated the mechanism(s) pertaining to the regulation of each step in the invasive process. Data presented in this thesis demonstrated that tumor cell invasion in HNSCC is a complex process involving three repeated sequential steps: adhesion, …

Bulletin 4369 - Ord River Irrigation Area - Kununurra Western Australia, Agriculture Western Australia

Bulletins 4000 -

The Ord River Irrigation Area (ORIA) in the Kimberley Province of Western Australia is the product of a bold and imaginative plan to develop part of the nation's tropical north for intensive agriculture by harnessing the waters of the Ord River. The principal features of the scheme are:

* A major storage reservoir formed by a dam constructed where the Ord River enters the Carr-Boyd Ranges, 160 kilometres south-east of Wyndham.

* A diversion dam, 50 kilometres further downstream, to raise the river level and divert water to be released from the major storage reservoir onto the irrigable lands.

* …

A Vibrio Cholerae Lysr Homolog, Aphb, Cooperates With Apha At The Tcpph Promoter To Activate Expression Of The Toxr Virulence Cascade, Gabriela Kovacikova, Karen Skorupski

Dartmouth Scholarship

We describe here a new member of the LysR family of transcriptional regulators, AphB, which is required for activation of the Vibrio cholerae ToxR virulence cascade. AphB activates the transcription of the tcpPH operon in response to environmental stimuli, and this process requires cooperation with a second protein, AphA. The expression of neither aphA or aphB is strongly regulated by environmental stimuli, raising the possibility that the activities of the proteins themselves may be influenced under various conditions. Strains of the El Tor biotype of V. cholerae typically exhibit lower expression of ToxR-regulated virulence genes in vitro than classical strains …

Field Evaluation Of Herbicides On Small Fruit, Vegetable, And Ornamental Crops, 1998, Ron E. Talbert, Lance A. Schmidt, Jennifer A. Wells

Arkansas Agricultural Experiment Station Research Series

Growers generally use herbicides to efficiently produce high-quality fruit and vegetables for processing or fresh market sales. Due to the smaller acreage of these crops compared to major field crops, fewer herbicides are registered for use in fruit and vegetable crops than for field crops. Each year, new herbicides are evaluated under Arkansas growing conditions with the objective of improving the herbicide technology for the grower, processor, and ultimately the consumer. This report includes studies on the control of many of the more serious weed problems in important crops of this region, including snapbeans, spinach, southernpeas, watermelon, cantaloupe, summer squash, …

Exploring Participation Processes For Technology Development: Case Studies Of Biotechnology Research And Development Projects In Thailand, Omjai Yuktavetya

Engineering Management & Systems Engineering Theses & Dissertations

There is increased awareness in the organizational and management literature that participation can make contributions in technology development. One of the problems in developing biotechnology in Thailand is that only a small portion of publicly funded research and development projects directly result in commercial success. Among the reasons cited (TDRI 1992a) is a lack of collaboration between various stakeholders; therefore, an effective technology policy to support their participation is needed. This research explores current participation processes in biotechnology research and development projects in Thailand in which there were different perspectives among various stakeholders. The quantitative and qualitative methodologies developed here …

Generation Of Human Chorionic Gonadotropin Beta Subunit Specific Monoclonal Antibody Using A Synthetic Peptide, Zhe Huang

Chemistry & Biochemistry Theses & Dissertations

Human chorionic gonadotropin (hCG) is well known for early immunological and bioassay tests for early detection of pregnancy. Since the hormone specific beta subunit confers unique biological and immunological specificity to hCG, immunochemical measurement of hCG has achieved greater specificity and sensitivity in recent years by using either the beta subunit as an immunogen or a partially reduced beta subunit polypeptide chain. However, antiserum raised against the entire hCG beta subunit usually cross-reacts to some extent with human luteinizing hormone (hLH). hCG has been found to be present in the extract of tumors and minute quantities of hCG are synthesized …

Cullin-3 Targets Cyclin E For Ubiquitination And Controls S Phase In Mammalian Cells, Jeffrey D. Singer, Mark Gurian-West, Bruce Clurman, James M. Roberts

Biology Faculty Publications and Presentations

Cyclin E is an unstable protein that is degraded in a ubiquitin- and proteasome- dependent pathway. Two factors stimulate cyclin E ubiquitination in vivo: when it is free of its CDK partner, and when it is phosphorylated on threonine 380. We pursued the first of these pathways by using a two-hybrid screen to identify proteins that could bind only to free cyclin E. This resulted in the isolation of human Cul-3, a member of the cullin family of E3 ubiquitin–protein ligases. We found that Cul-3 was bound to cyclin E but not to cyclin E-Cdk2 complexes in mammalian cells, and …

Dnak And The Heat Stress Response Of Pseudomonas Syringae Pv. Glycinea, Lisa M. W. Keith, James E. Partridge, Carol L. Bender

Department of Plant Pathology: Faculty Publications

The dnaK gene from Pseudomonas syringae pv. glycinea PG4180 was cloned and sequenced. The dnaK coding region was 1,917 bp and contained a putative O³² heat shock promoter 86 bp upstream of the translational start site. grpE, another heat shock gene, was found immediately upstream of the putative dnaK promoter. The predicted amino acid sequence of dnaK showed relatedness to the ATPase and substrate binding domains commonly found in heat shock proteins, as well as the highly conserved signature sequence motifs belonging to the Hsp70 protein family. Furthermore, the PG4180 dnaK gene complemented an Escherichia coli dnaK mutant …

Optimal Replication Activity Of Vesicular Stomatitis Virus Rna Polymerase Requires Phosphorylation Of A Residue(S) At Carboxy-Terminal Domain Ii Of Its Accessory Subunit, Phosphoprotein P, Leroy N. Hwang, Nathan Englund, Tapas Das, Amiya K. Florida, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The phosphoprotein, P, of vesicular stomatitis virus (VSV) is a key subunit of the viral RNA-dependent RNA polymerase complex. The protein is phosphorylated at multiple sites in two different domains. We recently showed that specific serine and threonine residues within the amino-terminal acidic domain I of P protein must be phosphorylated for in vivo transcription activity, but not for replication activity, of the polymerase complex. To examine the role of phosphorylation of the carboxy-terminal domain II residues of the P protein in transcription and replication, we have used a panel of mutant P proteins in which the phosphate acceptor sites …

Morphogenesis Is Coordinated With Nuclear Division In Germinating Aspergillus Nidulans Conidiospores, Steven D. Harris

Department of Plant Pathology: Faculty Publications

Germinating Aspergillus nidulans conidiospores switch to polarized apical growth following an initial period of isotropic expansion. At the same time, they re-enter the nuclear division cycle. The relationship between spore polarization and nuclear division was investigated by testing the effect of cell cycle inhibitors and temperature-sensitive cell cycle mutations on spore morphogenesis. On rich media, it was found that spore polarization is delayed if completion of the first mitosis is blocked. The observed delay may be dependent upon the activity of the mitosis-promoting NIMA kinase. An additional mechanism appears to prevent polarization as the spore progresses through its first S …

Canopy Analysis As A Technique To Characterize Defoliation Intensity On Sandhills Range, M. S. Miller-Goodman, Lowell E. Moser, Steven S. Waller, J. E. Brummer, P. E. Reece

Department of Agronomy and Horticulture: Faculty Publications

Characterization of relationships between grazing and vegetation responses is difficult. Rapid and accurate measurement of pasture canopy characteristics would help clarify these relationships if canopy changes are directly related to grazing variables. The objectives of this study were (1) to evaluate use of the LI-COR LAI-2000 for quantification of changes in canopy density and architecture in response to defoliation by cattle, (2) to determine if changes in leaf area index (LAI) measured with the LAI-2000 are related to stocking rate, and (3) to determine advantages and drawbacks of the LAI-2000 for monitoring grazing impacts on canopy density and architecture. Leaf …

Encystment, Excystment And Photosynthetic Activity In The Temporary Cysts Of Amphidinium Carterae, Steven R. Kibler

OES Theses and Dissertations

Although the formation of temporary cysts has been documented in several papers, the physiology of these stages has remained obscure. Research dealing with dinoflagellate resting stages has primarily targeted sexual cysts. Accordingly, formation, germination and photosynthetic activity in the temporary cysts of Amphidinium carterae have been investigated in the present study. The effect of light intensity upon cyst germination was tested by incubating temporary cysts at three different light intensities. In order to assess the potential for photosynthesis in cysts, cellular chlorophyll-a concentrations and cellular fluorescence were quantified during encystment. Photosynthetic carbon uptake of H¹⁴CO₃^- was …

Nitrate Reductase Activity, Nitrate Uptake And Iodine Speciation In The Marine Environment, Chin-Chang Hung

OES Theses and Dissertations

The present method for the determination of new production (NP) by measuring the uptake of added ¹⁵NO₃^– suffers from a number of limitations. In an attempt to improve this situation, this research examined the possibility of estimating ¹⁵NO₃– uptake by measuring the activity of nitrate reductase (NRA). In addition, because it has long been suspected that the biological reduction of IO₃^– to I^– may be mediated by nitrate reductase (NR), this research investigated the ability of NR to catalyze the reduction of iodate to iodide.

An improved method for the determination …

The Biological Flora Of Coastal Dunes And Wetlands. Ipomoea Imperati (Vahl) Griseb., Robert I. Lonard, Frank W. Judd

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Beach morning glory, Ipomoea imperati (Vahl) Griseb. = I. stolonifera (Cirillo) Gmelin is a pantropical, prostrate vine that is an important pioneer species in the backshore of coastal beaches of six continents and many islands. The landward distribution of beach morning glory is influenced by dispersal and competition, and its seaward distribution is determined by the physical environment acting through movement of seeds away from the water and high seedling mortality in the backshore. It is occasionally found in disturbed sites on barrier islands well inland from the shore. Beach morning glory tolerates low levels of soil nutrients, sand scouring, …

An Extracellular Protein Produced By Staphylococcus Hominis Strain Αm With Antimicrobial Activity Against Mycobacterium Spp, Sandra May Jacobsen

Biological Sciences Theses & Dissertations

The inhibitory activity exhibited by Staphylococcus hominis strain αM toward Mycobacterium species was examined. These studies included determining the conditions that maximized inhibitory agent production, analyzing characteristics of the agent, determining the time of agent production, ascertaining plasmid possession and the role of plasmids in the production of this inhibitory agent, and ascertaining the antimicrobial activity of other strains of S. hominis. On solid and in liquid media, S. hominis strain αM exhibits a unique inhibitory activity toward Mycobacterium species that is not demonstrated by Staphylococcus epidermidis or other S. hominis strains. Maximum agent production and recovery was …

Tissue-Specific Expression And Steroid Hormone Regulation Of Human Gonadotropin -Releasing Hormone (Hgnrh) Gene In Placental Cells (Jeg -3 Cells), Zhengguang Chen

Theses and Dissertations in Biomedical Sciences

Using JEG-3 cells as an in vitro model, I investigated the mechanisms behind the tissue-specific expression and steroid hormone regulation of the hGnRH gene in the human placenta. The hGnRH upstream promoter was found to be functionally active in JEG-3 cells. The DNA sequence responsible for functioning of the upstream promoter in JEG-3 cells is narrowed to a region between –1048 bp and –730 bp. This DNA fragment contains four elements, which can bind with nuclear extract from JEG-3 cells (but not from GT1-7 cells).

Estradiol (E2) represses the hGnRH upstream promoter activity in JEG-3 cells. This inhibition is receptor-mediated, …

Fraxinus Pennsylvanica Marsh., John E. E. Ebinger

Specimens by Name

No abstract provided.

Fraxinus Pennsylvanica Marsh., John E. E. Ebinger

Specimens by Name

No abstract provided.

Agricultural Research Division 113th Annual Report 1999

Agricultural Research Division: News and Annual Reports

It is a pleasure to provide you with a copy of the 113th Annual Report of the University of Nebraska Agricultural Research Division (ARD). This report is our opportunity to inform you about our research programs and accomplishments and to allow us to assess the progress and effectiveness of our collective efforts to develop new knowledge. After examining this report, we hope you will agree with our assessment that ARD research has provided new technology and knowledge for Nebraskans which will improve the profitability of their enterprises, enhance environmental quality, and improve their quality of life.

This report provides some …

Cholera Toxin Mediated Regulation Of The Expression Of Gq Alpha And G11 Alpha Gtp Binding Proteins, B H. Shah

Department of Biological & Biomedical Sciences

Previously it has been shown that persistent activation of the stimulatory adenylyl cyclase pathway with cholera toxin (CT) downregulates the Gs alpha polypeptide (80%) in a cAMP-independent manner in C6 glioma cells (Shah, 1997). This study was conducted to examine the short and long term effects of CT on the regulation of pertussis toxin-sensitive and -insensitive G proteins and their transcripts in C6 glioma cells. Treatment of C6 cells with CT (100 ng/ml) up to 16 h had no effect on either Gi or Gq/11 alpha proteins. However, prolonged exposure (24-48 h) caused increased expression of Gi (20-30%) and Gq/11 …

Molecular Techniques Reveal Wide Phyletic Diversity Of Heterotrophic Microbes Associated With Discodermia Spp. (Porifera: Demospongiae), Jose V. Lopez, Peter J. Mccarthy, Kathleen E. Janda, Robin Willoughby, Shirley A. Pomponi

Marine & Environmental Sciences Faculty Articles

Sponges are well known to harbor large numbers of heterotrophic microbes within their mesohyl. Studies to determine the diversity of these associated microbes have been attempted for only a few shallow water species. We cultured various microorganisms from several species of Discodermia collected from deep water using the 'Johnson-Sea-Link' manned submersibles, and characterised them by standard microbiological identification methods. Characterisation of a small proportion (ca. 10%) of the total and potential eubacterial isolate collection with molecular systematics techniques revealed a wide diversity of microbes. Phylogenetic analyses of 32 small subunit (SSU) 16S-like rRNA gene sequences from different micorbes indicated high …

Reproductive Biology Of Melilotus Officinalis (L.) Lam (Fabaceae: Papilionoideae): Morphology, Seed/Ovule Ratios And Ovule Abortion Patterns, Susan Lee Schouten

Dissertations and Theses

Melilotus officinalis, an introduced herbaceous species within the Papilionoideae, has a fixed ovule abortion pattern. In its primarily single-seeded, indehiscent fruits, with an average of 6.98 ovules/fruit, this species matured the basal-most ovule in 67.63% and the second basal-most ovule in 24.22% of the fruits studied. This pattern contrasts with other legumes studied, which abort the basal-most ovules or those at either end of the fruit. Workers propose interaction between two opposing gradients--that of maternal resources from the basal end of the fruit and of fertilization from the stylar end. Several lines of evidence indicate that all ovules are …

Providing The Edge For Small Businesses, Marilyn R. Schlake

Cornhusker Economics

Over the past decade, the explosive impact of small businesses on the United States economy has drawn the attention of policy makers and economic developers alike. And, with good reason! Information from the 1992 Small Business Administration Office of Advocacy showed that in Nebraska microbusinesses (businesses that employed less than 5 employees) accounted for more than 91% of all businesses in the state and contributed 20% of the state抯 payroll and proprietors?income. These businesses are located in communities and on the farms. They are auto repair shops, bakeries, jelly makers, tool and die shops, restaurants and crafters, just to name …

State Of The Fisheries Report 1998/1999, Jim W. Penn

Status Reports of the Fisheries and Aquatic Resources

The State of the Fisheries document is designed as an essential supplement to the Annual Report to Parliament and provides a more detailed level of information to support the agency's non-financial performance measures. By summarising management changes, compliance activities and research data such as landings, effort, catch rates, stock assessment and breeding stock levels, this document provides a valuable reference point for Western Australian fisheries ofmajor importance to the commercial, recreational and aquaculture sectors. Information relevant to Fisheries WA's Fish and Fish Habitat Protection Program and activities undertaken on behalf ofthe Commonwealth is also provided. The report indicates that overall …

Cropwatch No. 99-15, June 25, 1999, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Post control in sorghum………………132

Field updates………………134

Wheat diseases………………135

Sand chafers………………135

Imported weevils………………136

Corn diseases developing………………137

Purple loosetrife………………138

Stewart’s wilt………………139

Effects of weather………………140

Verbena Stricta Vent., Brian R. Garrard

Specimens by Name

No abstract provided.