Environmental Engineering Commons^™

Persistence Of Dna From Biocontained Genetically Engineered Microbes, Wentao Dai

McKelvey School of Engineering Graduate Student Theses & Dissertations

The utilization of genetically engineered microbes in environmental systems requires effective biocontainment mechanisms to ensure their safe operation. CRISPR-based kill switches are a promising solution, inducing cell death through site-specific DNA cleavage under specific environmental conditions. In this study, we aimed to assess the impact of CRISPR-based kill switches on the abundance and persistence of bacterial DNA. Our results revealed that while CRISPR-based kill switches were capable of achieving high reductions in viable Escherichia coli Nissle 1917 (EcN); the DNA largely remained intact. This was observed in two strains of EcN: one enabled with multi-locus genome cleavage, and the other …

Assessing The Ability Of Arduino-Based Sensor Systems To Monitor Changes In Water Quality, Josiah Hacker

Honors College Theses

Access to safe water is vital to public health. While developed countries like the United States are recognized as having reliable and safe water, many small water utilities struggle with supplying consistent water quality. Technicians of these utilities will periodically test water samples from the influent and throughout the distribution system. However, this laborious and costly process does not capture sudden changes in influent water quality due to environmental conditions or pipes breaking in the distribution system. Here I show how an Arduino-based sensor can be used as a real-time, low-cost monitor of water quality parameters. Specifically, I developed a …

Role Of Sediment Type, Concentration, And Salinity On Clay Flocs, Ryan Michael Johnson

Theses and Dissertations

Experiments were performed to study floc formation in water bodies. In particular, the study aimed to systematically identify effects of water depth, time, salinity, initial clay concentration, and clay type on floc formation and settling. Three sediment types were chosen for this research are Three sediment types were chosen for this research: silica powder, with particle sizes comparable to clay flocs, kaolin clay and bentonite clay. Silica was chosen as a control because it did not form flocs. Kaolin represented low activity clay; bentonite was chosen as the high activity clay. Experiments lasted up to 48 hours. Graduated cylinders were …

Unleash The Heat: Exploring Geothermal Energy Perspectives And Energy Literacy In São Miguel, Azores, Lena Mcdonough

Independent Study Project (ISP) Collection

The green energy transition is moving ahead in EU countries at very different paces, and there are some key challenges that all regions currently face in trying to phase fossil fuels out of their energy mixes. One of these challenges is that most regions simply cannot harness enough renewable energy sources and simultaneously have the storage technology for creating a baseload power source as reliable as oil, coal and natural gas. That is, unless you sit in the middle of the Atlantic Ocean on the boundary of three tectonic plates, with a continuous and reliable renewable energy source beneath your …

Drinking Water Contaminants: Bacteria, Bruce I. Dvorak, Becky O. Schuerman

Department of Civil and Environmental Engineering: Faculty Publications

Testing drinking water for fecal bacteria or Escherichia coli (E. coli) is commonly used as an indicator of the presence of pathogenic microorganisms. The presence of pathogenic (disease-causing) microorganisms is a concern when considering the safety of drinking water. Pathogenic microorganisms can cause flu-like systems, intestinal infections, dysentery, hepatitis, typhoid fever, cholera, and other illnesses.

Characterizing And Modeling The Impact Photorepair Conditions Have On Parameters Governing The Regrowth Of Escherichia Coli After Uvc Irradiation, And Investigating Potential Mitigation Strategies, Andrea J. Martinez Ballen

LSU Master's Theses

Ultraviolet disinfection has been used to treat water and wastewater for decades. Previous studies highlight the significance of disinfection conditions and attempt to model bacterial regrowth behavior according to disinfection dosing parameters. The emergence of light-emitting diode (LED) technology provides the ability to further explore bacterial repair methods after UV disinfection, which has been a growing concern within the UV community. However, it is necessary to also consider post-disinfection conditions to model the regrowth patterns of bacterial organisms more accurately. By varying reactivation wavelengths, and their respective intensities, experiments using Escherichia coli ATCC 15597 revealed how these parameters impact the …

Modeling Leachate Treatment Processes In Adsorbent-Amended Hybrid Constructed Wetland, Ishfaqun Nisa

USF Tampa Graduate Theses and Dissertations

Due to their efficiency in removing ammonia nitrogen, Constructed Wetlands (CW) are currently considered a very useful method for on-site leachate treatment. In recent years, the use of adsorbent media in CWs, such as zeolite and biochar, to improve pollutant removal efficiency has been explored and these studies indicate that incorporating zeolite can improve the nitrification process, while the addition of biochar can enhance the denitrification process and the removal of organic compounds. However, studies on mechanistic/process models simulating performance of adsorbent-amended CWs for treating landfill leachate are limited. This study presents simulation results for both unamended and adsorbent-amended hybrid …

Additive Manufacturing Of Engineered Cementitious Composites With Ultra-High Tensile Ductility, Amir Bakhshi

Civil Engineering ETDs

One of the critical factors in the development of additive manufacturing in the construction industry is designing suitable materials for 3D printing applications. 3D concrete printing (3DCP) has gained significant attention due to the abundance and availability of concrete. However, concrete is a brittle material and, without proper reinforcement, cannot meet the requirements for structural purposes. Engineered Cementitious Composites (ECC), known for high ductility, and strain-hardening with low fiber contents, can potentially eliminate the need for steel reinforcement in 3D printing without any need for manual placement of reinforcement. A set of systematic tests on ECC mix designs' fresh and …

Field-Base Exploratory Study Of Microbial Activity In Eight Potable Water Storage Tanks In Barbados, Katelyn M. Long

USF Tampa Graduate Theses and Dissertations

There have been many initiatives to combat the global phenomenon of water scarcity. Potable water storage tanks have become widely known among water insecure countries and communities to provide adequate water quantity and quality for consumer use. Despite ubiquitous use in many low- and middle-income communities (LMIC), knowledge of microbial activity inside potable water storage tanks is limited. Barbados is a Small Island Developing State (SIDS), labeled as a high income country, and also water scarce. The Barbados Water Authority (BWA) has adapted potable water storage tanks for hundreds of residents as a solution to water quantity and water quality …

Disturbances In Primary Visual Processing As A Function Of Healthy Aging, Seth D. Springer, Tara D. Erker, Mikki Schantell, Hallie J. Johnson, Madelyn P. Willett, Hannah J. Okelberry, Maggie P. Rempe, Anthony Wilson

Department of Agricultural and Biological Systems Engineering: Faculty Publications

For decades, visual entrainment paradigms have been widely used to investigate basic visual processing in healthy individuals and those with neurological disorders. While healthy aging is known to be associated with alterations in visual processing, whether this extends to visual entrainment responses and the precise cortical regions involved is not fully understood. Such knowledge is imperative given the recent surge in interest surrounding the use of flicker stimulation and entrainment in the context of identifying and treating Alzheimer’s disease (AD). In the current study, we examined visual entrainment in eighty healthy aging adults using magnetoencephalography (MEG) and a 15 Hz …

Evaluating The Environmental Impacts Of U.S. Historical Oil Spill Incidents, Yiming Liu, Hua Cai

Graduate Industrial Research Symposium

The adverse impacts of extremely large oil spill incidents such as Deepwater Horizon Oil Spill have been widely studied in recent years. Catastrophic oil spill disasters such as Deepwater Horizon Oil Spill have been widely studied. While vast smaller oil spills, which also could result in significant amount of oil release, still lack attention.

This research assessed distributions and environmental impacts caused by historical oil spill incidents in U.S. We first analyzed the spatial and temporal characteristics of oil spills. Then the relationship between spillage amount and spill frequency is evaluated via statistical approaches. Last, based on a thorough literature …

Comprehensive Microenvironment Monitoring For The Productivity And Welfare Of Sows And Piglets, Jun Ho Kim

Graduate Industrial Research Symposium

The world is facing increasing food demand and climate change. To improve piglet survivability and sow welfare through microenvironment management using precision technology, a USDA sponsored research project started in 2021 and is ongoing. A comprehensive yet low-cost system was designed and developed for continuous and real-time microenvironment monitoring in three rooms of Farrowing Building #1 at the Animal Sciences Research and Education Center, Purdue University. The system consists of two gas analyzers and 103 different sensors, including sensors in a weather station. The first trial of the project was conducted from Jan. 13 to Feb. 8, 2023, when the …

Rehabilitation Technologies To Abate Infiltration In Sanitary Sewers, Steve Youssef

USF Tampa Graduate Theses and Dissertations

In urban coastal environments, sewer infrastructure is plagued by infiltration which seeps into aging and compromised pipes. Infiltration increases pumping costs, reduces treatment efficiency, and may trigger release of partially treated wastewater at water reclamation facilities. To restore pipes and abate infiltration, local utilities rely on structural and non-structural trenchless rehabilitation technologies. These methods are commonly used throughout the United States and the world due to their numerous benefits compared to traditional open-cut construction. However, limited data is available regarding their efficacy in reducing infiltration in sewer systems. In this study, we analysed infiltration in a small urban sewershed in …

The Effect Of Relative Humidity On Eddy Covariance Latent Heat Flux Measurements And Its Implication For Partitioning Into Transpiration And Evaporation, Weijie Zhang, Martin Jung, Mirco Migliavacca, Rafael Poyatos, Diego G. Miralles, Tarek S. El-Madany, Marta Galvagno, Arnaud Carrara, Nicola Arriga, Andreas Ibrom, Ivan Mammarella, Dario Papale, Jamie R. Cleverly, Michael Liddell, Georg Wohlfahrt, Christian Markwitz, Matthias Mauder, Eugenie Paul-Limoges, Marius Schmidt, Sebastian Wolf, Christian Brümmer, M. Altaf Arain, Silvano Fares, Tomomichi Kato, Jonas Ardö, Walter Oechel, Chad Hanson, Mika Korkiakoski, Sébastien Biraud, Rainer Steinbrecher, Dave Billesbach, Leonardo Montagnani, William Woodgate, Changliang Shao, Nuno Carvalhais, Markus Reichstein, Jacob A. Nelson

Department of Agricultural and Biological Systems Engineering: Faculty Publications

While the eddy covariance (EC) technique is a well-established method for measuring water fluxes (i.e., evaporation or 'evapotranspiration’, ET), the measurement is susceptible to many uncertainties. One such issue is the potential underestimation of ET when relative humidity (RH) is high (>70%), due to low-pass filtering with some EC systems. Yet, this underestimation for different types of EC systems (e.g. open-path or closed-path sensors) has not been characterized for synthesis datasets such as the widely used FLUXNET2015 dataset. Here, we assess the RH-associated underestimation of latent heat fluxes (LE, or ET) from different EC systems for 163 sites in …

Environmental Trade-Offs Of Reverse Osmosis Water Purification Units (Rowpus) And Bottled Water, Caleb A. Boone

Theses and Dissertations

The Department of Defense (DoD) conducts many remote missions around the globe. As part of any remote basing strategy, potable water needs to be secured. Currently, there are three primary means of procuring potable water at remote locations: bottled water, on-site purification, or tie-in to existing, local infrastructure. The purpose of this study is to determine the environmental tradeoffs of bottled water and on-site treatment via a Reverse Osmosis Water Purification Unit (ROWPU), which uses multiple levels of filtration to make potable water from freshwater or saline water. A cradle-to- gate assessment was developed for both systems to create a …

Temporal And Spatial Variability Of Specific Energy Consumption For Atmospheric Water Generators, Anthony T. Brenes

Theses and Dissertations

Atmospheric Water Generators (AWG) produce potable water from the moisture in the air, providing a potentially viable water source in austere locations or emergency response scenarios. In this study, the operating constraints of three existing commercially available AWG devices are investigated, compared to historical weather data from across the continental United States. Utilizing linear regression modeling and weather station data for the years of 1985-2019, the monthly and spatial trends of energy demand to produce water from these devices are evaluated. Energy and water production efficiencies for the devices are highly dependent on environmental conditions with relative humidity and temperature …

Investigation Of Possible Alternatives To Fossil Fuels For The Dod In Indopacom, Terrence W. Caploe

Theses and Dissertations

Energy security, greenhouse gas (GHG) emissions, and geopolitical concerns in the south pacific require diversification and independence from legacy energy sources in the Indo-Pacific Combatant Command (INDOPACOM) area of operation (AO). The United States Government has placed mandates and directives to minimize the impacts of GHG on the climate. These orders require divestment from GHG producing fuel sources by 2050. This study compares the energy alternates to Jet Propulsion 8 (JP8) as Ammonia, Hydrogen, and Lithium-ion Batteries. The study uses Life Cycle Assessment (LCA) tools to determine the environmental impacts of various energy candidates in the South Pacific. These results …

The Kinetics Of Biocontaminant Inactivation: A Comparative Study With Bacillus Globigii And Bacillus Anthracis, Leigh M. Durden

Theses and Dissertations

Bacillus anthracis has been a biological threat for decades and after the 2001 terrorist attack via the U.S. Postal Service where five people died, there was heightened awareness and inquiry on the subject (Goel, 2015; Greenberg et al., 2010; Nicholson & Galeano, 2003; Spotts Whitney et al., 2003). The health risk to humans makes this biological agent, a Tier 1 agent and one of the most likely to be used in an attack (Anthrax as a Bioterrorism Weapon | CDC, n.d.; Driks, 2009; Rose & Rice, 2014). B. anthracis is one of most resistant and potent biological warfare agents as …

Net Zero Water Air Force Installations, Kenneth R. Mcknight

Theses and Dissertations

The United States continues to face problems of a reduction in quality and quantity of groundwater sources because water extraction exceeds natural source recharge. The Air Force has recognized the importance of these groundwater sources but has put minimal effort into determining their contribution to the depletion of these sources. The purpose of this study is to determine this contribution by determining whether Air Force installations are net zero water. This is done using a geospatial information system to determine the volume of water recharging groundwater sources associated with an Air Force installation. This volume is then compared to the …

Decontamination Of Perfluoroalkyl Substances From Stainless Steel Piping Used In Aqueous Film Forming Foam Fire Suppression Systems, Ryan M. Register

Theses and Dissertations

This study examines the removal of perfluoroalkyl substances (PFAS) from legacy hangar fire suppression systems which utilized PFAS containing aqueous film forming foam (AFFF). Hundreds of these systems are currently in place throughout the Department of Defense, with planned phase-out occurring by 2024. The estimated cost of replacing these systems is $2.1 billion dollars. To mitigate this cost, a triple-rinse method of decontamination has been proposed. This thesis explores the release of PFAS from AFFF systems during a triple-rinse decontamination, and the continued removal of PFAS after a triple-rinse is complete while simultaneously analyzing methylene blue dye as a potential …

Steam And Environmental Justice In An Interdisciplinary Context, Paula Farca, Alina Handorean, Jürgen Brune

The Transdisciplinary STEAM+ Journal

This course proposes an interdisciplinary perspective, envisions unique synergies between environmental justice concepts and STEAM projects on mining, and aims to solidify a foundation based on justice, equity, equality, and empathy for STEM students and faculty. Our (S)TEAM made of professors in three academic departments underscores interdisciplinary and diversity connections through an interdisciplinary team-taught course, units on environmental justice related to mining, teaching of literary texts, and STEAM projects. We also involved faculty, alumni, and our campus and city community through STEAM exhibits.

Enhancing Performance Of Gravity Driven Membranes For Water Purification In Small Rural Communities, Amr Ezzat Melligy Mr.

Theses and Dissertations

Due to the rapid population growth in Egypt, providing safe drinking water is becoming an ongoing challenge especially in rural scattered communities. It became more crucial than ever to find innovative and affordable solutions to purify water and reduce water-borne diseases. Ultrafiltration and micro-filtration membranes has been successful in large, centralized plants and is currently one of the popular solutions to provide high quality product water. However, using those membrane systems in rural areas is challenged by several parameters; among which are; the absence of skilled workers, stable energy sources and availability of chemicals. The goal of this thesis is …

Secreted Metabolome Of Porcine Blastocysts Encapsulated With In In Vitro 3d Alginate Hydrogel Culture Systems Under Going Morphological Changes Provides Insights Into Specific Mechanisms Involved In The Initiation Of Porcine Conceptus Elongation, Sophie C. Walsh, Jeremy R. Miles, Corey D. Broeckling, Lea A. Rempel, Elane C. Wright-Johnson, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Context. The exact mechanisms regulating the initiation of porcine conceptus elongation are not known due to the complexity of the uterine environment. Aims. To identify contributing factors for initiation of conceptus elongation in vitro, this study evaluated differential metabolite abundance within media following culture of blastocysts within unmodified alginate (ALG) or Arg-Gly-Asp (RGD)-modified alginate hydrogel culture systems. Methods. Blastocysts were harvested from pregnant gilts, encapsulated within ALG or RGD or as non-encapsulated control blastocysts (CONT), and cultured. At the termination of 96 h culture, media were separated into blastocyst media groups: non-encapsulated control blastocysts (CONT); ALG …

Process Intensification Strategies For Green Solvent Mediated Biomass Pretreatment, Ahamed Ullah, Yuxuan Zhang, Can Liu, Qi Qiao, Qing Shao, Jian Shi

Markey Cancer Center Faculty Publications

Demonstrated to be highly effective for lignocellulosic biomass pretreatment, deep eutectic solvent (DES) has attracted increasing attention owing to its advantages of simple synthesis, relatively low chemical cost, and better biocompatibility as compared to certain ionic liquids. Here we provide a critical review of the status of the design/selection of DES for the pretreatment of biomass feedstocks with an emphasis on the process intensification strategies: 1) integration of microwave, ultrasound, and high solid extrusion for pretreating biomass, 2) one-pot DES pretreatment, enzymatic hydrolysis, and fermentation, 3) strategies for DES recycling and product recovery; and 4) recent progress on molecular simulations …

Estimating Solar Energy Production In Urban Areas For Electric Vehicles, Shaimaa Ahmed

Theses and Dissertations

Cities have a high potential for solar energy from PVs installed on buildings' rooftops. There is an increased demand for solar energy in cities to reduce the negative effect of climate change. The thesis investigates solar energy potential in urban areas. It tries to determine how to detect and identify available rooftop areas, how to calculate suitable ones after excluding the effects of the shade, and the estimated energy generated from PVs. Geographic Information Sciences (GIS) and Remote Sensing (RS) are used in solar city planning. The goal of this research is to assess available and suitable rooftops areas using …

Co-Culture Models Of Endothelial Cells, Macrophages, And Vascular Smooth Muscle Cells For The Study Of The Natural History Of Atherosclerosis, Martin Liu, Saurabhi Samant, Charu Hasini Vasa, Ryan M. Pedrigi, Usama Oguz, Sangjin Ryu, Timothy Wei, Daniel R. Anderson, Devandra K. Agrawal, Yiannis Chatzizisis

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background

This work aims to present a fast, affordable, and reproducible three-cell co-culture system that could represent the different cellular mechanisms of atherosclerosis, extending from atherogenesis to pathological intimal thickening.

Methods and results

We built four culture models: (i) Culture model #1 (representing normal arterial intima), where human coronary artery endothelial cells were added on top of Matrigel-coated collagen type I matrix, (ii) Culture model #2 (representing atherogenesis), which demonstrated the subendothelial accumulation and oxidative modification of low-density lipoproteins (LDL), (iii) Culture model #3 (representing intimal xanthomas), which demonstrated the monocyte adhesion to the endothelial cell monolayer, transmigration into the …

Virtual Accident Curb Risk Habituation In Workers By Restoring Sensory Responses To Real-World Warning, Namgyun Kim, Laurent Grégoire, Moein Razavi, Niya Yan, Changbum R. Ahn, Brian A. Anderson

Civil and Environmental Engineering and Engineering Mechanics Faculty Publications

In high-risk work environments, workers become habituated to hazards they frequently encounter, subsequently underestimating risk and engaging in unsafe behaviors. This phenomenon has been termed "risk habituation"and identified as a vital root cause of fatalities and injuries at workplaces. Providing an effective intervention that curbs workers' risk habituation is critical in preventing occupa-tional injuries and fatalities. However, there exists no empirically supported intervention for curbing risk habituation. To this end, here we investigated how experiencing an accident in a virtual reality (VR) environment affects workers' risk habituation toward repeatedly exposed workplace hazards. We examined an underlying mechanism of risk habituation …

Anaerobic Toluene Biodegradation Potential At A Superfund Site Where Toluene Biosynthesis Was Observed, George Carson

LSU Master's Theses

At a Superfund site near Baton Rouge, LA, the subsurface injection of molasses has been used to stimulate anerobic reductive dechlorination of halogenated organic compounds (e.g., 1,2-dichloroethane and vinyl chloride) present in the groundwater. Following some molasses injections, temporary accumulation of toluene and p-cresol was observed in the groundwater. Accumulation of these aromatic hydrocarbons, which were not detected in the molasses, has been attributed to anaerobic biotransformation of the amino acids phenylalanine and tyrosine. Experiments reported here were conducted in support of an effort to determine if toluene biodegrading bacteria were present in the area where anaerobic toluene accumulation was …

Greywater Treatment Using Integrated Sand Filtration With Ultrafiltration For Reuse, Aya El-Wattar

Theses and Dissertations

Membrane flux was observed and the two membrane sizes that achieved stable flux were chosen to be used in the later stages of the study. The chosen membranes were of 300 kDa and 400 kDa Molecular Weight Cutoff (MWCO).

The third phase of the study involved running the full system on synthetic greywater then on real greywater. The parameters investigated were COD, TSS, Turbidity and BOD₅. Total COD removal after sand filtration, 300kDa UF and 400 kDa UF was 65, 66 and 73.4% respectively. Total TSS removal after sand filtration, 300kDa UF and 400 kDa UF was 93.1, …

Soil Water Sensors For Irrigation Scheduling In The United States: A Systematic Review Of Literature, Sumon Datta, Saleh Taghvaeian

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Soil water sensors (SWS) have been widely researched and practiced for irrigation scheduling. Due to popularity of this approach, there is a need for frequent reviews of the SWS literature to keep the researchers, extension professionals, and end-users updated and to help with defining and modifying future directions. In addition, existing literature reviews do not systematically report and summarize the results of previous studies. To address this gap, a systematic review was carried out to identify and analyze related literature published in the United States in the past two decades. Two focus areas were SWS accuracy assessment and SWS application …