Engineering Commons^™

Upregulation Of Dna Sensors In B16.F10 Melanoma Spheroid Cells After Electrotransfer Of Pdna, Katarina Znidar, Masa Bosnjak, Tanja Jesenko, Loree C. Heller, Maja Cemazar

Bioelectrics Publications

Increased expression of cytosolic DNA sensors, a category of pattern recognition receptor, after control plasmid DNA electrotransfer was observed in our previous studies on B16.F10 murine melanoma cells. This expression was correlated with the upregulation of proinflammatory cytokines and chemokines and was associated with cell death. Here, we expanded our research to include the influence of features of cells in a 3-dimensional environment, which better represents the tumors’ organization in vivo. Our results show that lower number of cells were transfected in spheroids compared to 2-dimensional cultures, that growth was delayed after electroporation alone or after electrotransfer of plasmid …

Excitation And Injury Of Adult Ventricular Cardiomyocytes By Nano- To Millisecond Electric Shocks, Iurii Semenov, Sergey Grigoryev, Johanna U. Neuber, Christian W. Zemlin, Olga N. Pakhomova, Maura Casciola, Andrei G. Pakhomov

Bioelectrics Publications

Intense electric shocks of nanosecond (ns) duration can become a new modality for more efficient but safer defibrillation. We extended strength-duration curves for excitation of cardiomyocytes down to 200 ns, and compared electroporative damage by proportionally more intense shocks of different duration. Enzymatically isolated murine, rabbit, and swine adult ventricular cardiomyocytes (VCM) were loaded with a Ca²⁺ indicator Fluo-4 or Fluo-5N and subjected to shocks of increasing amplitude until a Ca²⁺ transient was optically detected. Then, the voltage was increased 5-fold, and the electric cell injury was quantified by the uptake of a membrane permeability marker dye, propidium …

Nano-Pulse Stimulation For The Treatment Of Pancreatic Cancer And The Changes In Immune Profile, Sigi Guo, Niculina I. Burcus, James Hornef, Yu Jing, Chunqi Jiang, Richard Heller, Stephen J. Beebe

Bioelectrics Publications

A Pancreatic cancer is a notorious malignant neoplasm with an extremely poor prognosis. Current standard of care is rarely effective against late-stage pancreatic cancer. In this study, we assessed nanopulse stimulation (NPS) as a local treatment for pancreatic cancer in a syngeneic mouse Pan02 pancreatic cancer model and characterized corresponding changes in the immune profile. A single NPS treatment either achieved complete tumor regression or prolonged overall survival in animals with partial tumor regression. While this is very encouraging, we also explored if this local ablation effect could also result in immune stimulation, as was observed when NPS led to …

Dielectric Properties Of Isolated Adrenal Chromaffin Cells Determined By Microfluidic Impedance Spectroscopy, A. C. Sabuncu, M. Stacey, G. L. Graviso, N. Semenova, P. T. Vernier, N. Leblanc, I. Chatterjee, J. Zaklit

Bioelectrics Publications

Knowledge of the dielectric properties of biological cells plays an important role in numerical models aimed at understanding how high intensity ultrashort nanosecond electric pulses affect the plasma membrane and the membranes of intracellular organelles. To this end, using electrical impedance spectroscopy, the dielectric properties of isolated, neuroendocrine adrenal chromaffin cells were obtained. Measured impedance data of the cell suspension, acquired between 1 kHz and 20 MHz, were fit into a combination of constant phase element and Cole-Cole models from which the effect of electrode polarization was extracted. The dielectric spectrum of each cell suspension was fit into a Maxwell-Wagner …

Dynamic Response Modeling Of High Speed Planing Craft With Enforced Acceleration, Brian K. Johnson

Mechanical & Aerospace Engineering Theses & Dissertations

Due to the harsh conditions high speed planing crafts must endure, research to further the understanding of high speed vessel response during wave impacts was conducted. The integration of a finite element model and captured sea trial acceleration data was investigated. The research shows that the finite element model sub-model can be used in lieu of a full finite element model with minimum degradation in output, thus allowing for the analysis of local stress concentrations where critical equipment and or personnel may be located.

The research effort was completed to develop a method for realizing the stress field and deformation …

A Novel Brainstorming Pedagogy To Mobilize Pico/Nano/Micro-Satellite (Pn-Msat) Engineering Research And Education In Indian Academia, Sharanabasaweshwara Asundi

Mechanical & Aerospace Engineering Faculty Publications

The article describes the outcome of activities to positively impact the careers of engineering graduates in India by engaging them in pico/nano/micro-satellite (PNMSat) engineering through a novel brainstorming pedagogy. The pedagogy, derived out of a systems engineering approach developed for the design and development of PNMSat/CubeSat missions, is used to teach a comprehensive course in PNMSat design engineering. The approach involves brainstorming the participants to conceive a PNMSat payload and teach the PNMSat bus design to accommodate the conceived payload. The approach and the comprehensive treatment of the material are the first of their kind in India in the field …

Electrical Response Of Thermoelectric Generator To Geometry Variation Under Transient Thermal Boundary Condition, Elias Yazdanshenas, Alireza Rezania, Meysam Karami Rad, Lasse Rosendahl

Mechanical & Aerospace Engineering Faculty Publications

A three-dimensional numerical model is applied in this study to illustrate the electrical response of a thermoelectric generator (TEG) during transient heat flux at the hot side. In this work, various types of thermal boundary conditions are considered to evaluate the performance of the TEG. Thus, a TEG under pulsed heat flux is studied numerically, and the numerical model is verified by experimental results. With the consideration of a defined reference geometry, different heat flux frequencies are applied in order to evaluate the corresponding electrical output by the TEG. In addition, variation of the module performance for various TEG leg …

Electrotransfer Of Different Control Plasmids Elicits Different Antitumor Effectiveness In B16.F10 Melanoma, Masa Bosnjak, Tanjo Jesenko, Urska Kamensek, Gregor Sersa, Jaka Lavrencak, Loree Heller, Maja Cemazar

Bioelectrics Publications

Several studies have shown that different control plasmids may cause antitumor action in different murine tumor models after gene electrotransfer (GET). Due to the differences in GET protocols, plasmid vectors, and experimental models, the observed antitumor effects were incomparable. Therefore, the current study was conducted comparing antitumor effectiveness of three different control plasmids using the same GET parameters. We followed cytotoxicity in vitro and the antitumor effect in vivo after GET of control plasmids pControl, pENTR/U6 scr and pVAX1 in B16.F10 murine melanoma cells and tumors. Types of cell death and upregulation of selected cytosolic DNA sensors and cytokines were …

Nanopulse Stimulation (Nps) Induces Tumor Ablation And Immunity In Orthotopic 4t1 Mouse Breast Cancer: A Review, Stephen J. Beebe, Brittany P. Lassiter, Siqi Guo

Bioelectrics Publications

Nanopulse Stimulation (NPS) eliminates mouse and rat tumor types in several different animal models. NPS induces protective, vaccine-like effects after ablation of orthotopic rat N1-S1 hepatocellular carcinoma. Here we review some general concepts of NPS in the context of studies with mouse metastatic 4T1 mammary cancer showing that the postablation, vaccine-like effect is initiated by dynamic, multilayered immune mechanisms. NPS eliminates primary 4T1 tumors by inducing immunogenic, caspase-independent programmed cell death (PCD). With lower electric fields, like those peripheral to the primary treatment zone, NPS can activate dendritic cells (DCs). The activation of DCs by dead/dying cells leads to increases …

Analyzing The Spectral Energy Cascade In Turbulent Channel Flow, João Rodrigo Andrade, Ramon Silva Martins, Gilmar Mompean, Laurent Thais, Thomas B. Gatski

CCPO Publications

An analysis of the spectral turbulent kinetic energy budget in a fully developed turbulent plane channel flow is performed. Direct numerical simulation data are evaluated at friction Reynolds numbers Re_τ of 180 and 1000. The analysis is focused on the influence of the Reynolds number on the spectral cascade of energy and the corresponding energy cascade in physical space in the presence of inhomogeneity and anisotropy. The turbulent kinetic energy distribution is compared for both Reynolds numbers, and the relevant characteristics of the energy transfer process in a wall-bounded turbulent flow are described. Differences in energy cascade are noted …

Forecasting Changes In Religiosity And Existential Security With An Agent-Based Model, Ross J. Gore, Carlos Lemos, F. Leron Shults, Wesley J. Wildman

VMASC Publications

We employ existing data sets and agent-based modeling to forecast changes in religiosity and existential security among a collective of individuals over time. Existential security reflects the extent of economic, socioeconomic and human development provided by society. Our model includes agents in social networks interacting with one another based on the education level of the agents, the religious practices of the agents, and each agent's existential security within their natural and social environments. The data used to inform the values and relationships among these variables is based on rigorous statistical analysis of the International Social Survey Programme Religion Module (ISSP) …

Combined Effect Of Storm Surge And Overland Flow On Flooding In A Coastal Urban Area, Navid Tahvildari, Akash Sahu, Yawen Shen, Mohamed Morsy, Jonathan Goodall

Civil & Environmental Engineering Faculty Publications

The coastal regions in the U.S. East Coast and the Gulf of Mexico are under the risk of storm surge and precipitation-driven flooding. The adverse impacts of climate change including sea level rise (SLR), potential increase in intensity and frequency of extreme storms, and increase in precipitation intensity increases the vulnerability of coastal communities to flooding. The common practice for flood hazard assessment in urban coastal areas can result in some errors as the effect of storm surge and overland flow are not considered simultaneously. In this study, we combine the results of two hydrodynamic models, one for overland flow …

Towards Blockchain Empowered Trusted And Accountable Data Sharing And Collaboration In Mobile Healthcare Applications, Xueping Liang, Sachin Shetty, Deepak Tosh, Daniel Bowden, Laurent Njilla, Charles Kamhoua

VMASC Publications

Enabled by mobile and wearable technology, personal health data delivers immense and increasing value for healthcare, benefiting both care providers and medical research. The secure and convenient sharing of personal health data is crucial to the improvement of the interaction and collaboration of the healthcare industry. Faced with the potential privacy issues and vulnerabilities existing in current personal health data storage and sharing systems, as well as the blockchain integration concerns summarized in this paper, an innovative user-centric health data sharing solution by utilizing a decentralized but permissioned blockchain is proposed to protect privacy and enhance access management, with the …

Targeting Ovarian Cancer And Endothelium With An Allosteric Ptp4a3 Phosphatase Inhibitor, Kelley E. Mcqueeney, Joseph M. Salamoun, James C. Burnett, Nektarios Barabutis, Paula Pekic, Sophie L. Lewandowski, Danielle C. Llaneza, Robert Cornelison, Yunpeng Bai, Zhong-Yin Zhang, John D. Catravas

Bioelectrics Publications

Overexpression of protein tyrosine phosphatase PTP4A oncoproteins is common in many human cancers and is associated with poor patient prognosis and survival. We observed elevated levels of PTP4A3 phosphatase in 79% of human ovarian tumor samples, with significant overexpression in tumor endothelium and pericytes. Furthermore, PTP4A phosphatases appear to regulate several key malignant processes, such as invasion, migration, and angiogenesis, suggesting a pivotal regulatory role in cancer and endothelial signaling pathways. While phosphatases are attractive therapeutic targets, they have been poorly investigated because of a lack of potent and selective chemical probes. In this study, we disclose that a potent, …

Transport Of Charged Small Molecules After Electropermeabilization - Drift And Diffusion, Esin B. Sözer, C. Florencia Pocetti, P. Thomas Vernier

Bioelectrics Publications

Background: Applications of electric-field-induced permeabilization of cells range from cancer therapy to wastewater treatment. A unified understanding of the underlying mechanisms of membrane electropermeabilization, however, has not been achieved. Protocols are empirical, and models are descriptive rather than predictive, which hampers the optimization and expansion of electroporation-based technologies. A common feature of existing models is the assumption that the permeabilized membrane is passive, and that transport through it is entirely diffusive. To demonstrate the necessity to go beyond that assumption, we present here a quantitative analysis of the post-permeabilization transport of three small molecules commonly used in electroporation research-YO-PRO-1, propidium, …

The Effect Of Tackling Training On Head Accelerations In Youth American Football, Eric Schussler, Richard J. Jagacinski, Susan E. White, Ajit M. Chaudhari, John A. Buford, James A. Onate

Rehabilitation Sciences Faculty Publications

Background: Many organizations have introduced frameworks to reduce the incidence of football related concussions through proper equipment fitting, coach education, and alteration of tackling technique.

Purpose: The purpose of this study was to examine the effects of training in a vertical, head up tackling style on the number of head accelerations experienced while tackling in a controlled laboratory situation. The authors hypothesized that training in a head up tackling technique would reduce the severity of head acceleration experienced by participants.

Design: Controlled Laboratory Study.

Methods: Twenty-four participants (11.5 ± 0.6 years old, 60.5 ± 2.2 in, 110 ± 18.4 lbs.) …

Effect Of Curing Rate On The Microstructure And Macroscopic Properties Of Epoxy Fiberglass Composites, Ammar Patel, Olesksandr Kravchenko, Ica Manas-Zloczower

Mechanical & Aerospace Engineering Faculty Publications

Curing rates of an epoxy amine system were varied via different curing cycles, and glass-fiber epoxy composites were prepared using the same protocol, with the aim of investigating the correlation between microstructure and composite properties. It was found that the fast curing cycle resulted in a non-homogenous network, with a larger percentage of a softer phase. Homogenized composite properties, namely storage modulus and quasi-static intra-laminar shear strength, remained unaffected by the change in resin microstructure. However, fatigue tests revealed a significant reduction in fatigue life for composites cured at fast curing rates, while composites with curing cycles that allowed a …

Fpga-Based On-Board Geometric Calibration For Linear Ccd Array Sensors, Guoqing Zhou, Linjun Jiang, Jingjin Huang, Rongting Zhang, Dequan Liu, Xiang Zhou, Oktay Baysal

Mechanical & Aerospace Engineering Faculty Publications

With increasing demands in real-time or near real-time remotely sensed imagery applications in such as military deployments, quick response to terrorist attacks and disaster rescue, the on-board geometric calibration problem has attracted the attention of many scientists in recent years. This paper presents an on-board geometric calibration method for linear CCD sensor arrays using FPGA chips. The proposed method mainly consists of four modules—Input Data, Coefficient Calculation, Adjustment Computation and Comparison—in which the parallel computations for building the observation equations and least squares adjustment, are implemented using FPGA chips, for which a decomposed matrix inversion method is presented. A Xilinx …

Fostering Giftedness And Creativity: Implementing Engineering By Design In Kuwait, Nathan Mentzer, Philip A. Reed, Meshari Alnouri, Mohammad Barbarji

STEMPS Faculty Publications

The article offers information on a partnership between the International Technology & Engineering Educators Association (ITEEA) and the Sabah Al Ahmad Center for Giftedness and Creativity (SACGC) to support innovation and design education. The partnership is aimed towards the implementation of the Engineering byDesign (EbD) curriculum and contribute towards building a Kuwaiti society that fosters giftedness and creativity.

Electrowetting Using A Microfluidic Kelvin Water Dropper, Elias Yazdanshenas, Qiang Tang, Xiaoyu Zhang

Mechanical & Aerospace Engineering Faculty Publications

The Kelvin water dropper is an electrostatic generator that can generate high voltage electricity through water dripping. A conventional Kelvin water dropper converts the gravitational potential energy of water into electricity. Due to its low current output, Kelvin water droppers can only be used in limited cases that demand high voltage. In the present study, microfluidic Kelvin water droppers (MKWDs) were built in house to demonstrate a low-cost but accurately controlled miniature device for high voltage generation. The performance of the MKWDs was characterized using different channel diameters and flow rates. The best performed MKWD was then used to conduct …

Tuskegee University Amateur Radio Station- Vhf/Uhf Digital Communication With Legacy Systems, Sharanabasaweshwara A. Asundi, Sharath S. Byakod

Mechanical & Aerospace Engineering Faculty Publications

No abstract provided.

A Reliable Data Provenance And Privacy Preservation Architecture For Business-Driven Cyber-Physical Systems Using Blockchain, Xueping Liang, Sachin Shetty, Deepak K. Tosh, Juan Zhao, Danyi Li, Jihong Liu

VMASC Publications

Cyber-physical systems (CPS) including power systems, transportation, industrial control systems, etc. support both advanced control and communications among system components. Frequent data operations could introduce random failures and malicious attacks or even bring down the whole system. The dependency on a central authority increases the risk of single point of failure. To establish an immutable data provenance scheme for CPS, the authors adopt blockchain and propose a decentralized architecture to assure data integrity. In business-driven CPS, end users are required to share their personal information with multiple third parties. To prevent data leakage and preserve user privacy, the authors isolate …

Introduction: Metrics, Modeling And Simulation For Cyber Physical Systems, Barry Ezell, Luanne Burns

VMASC Publications

[Introduction] In recent years, there has been a massive increase in cyber crime. Cyber breeches have become ubiquitous. The US government is attempting to adapt through legislation and policy. For instance, business in the defense industrial base must be in compliance of NIST 80-71 by 31 December 2017. Insurance companies as well are beginning to build actuaries on cyber risk. Along with NIST, there are many cyber frameworks that exist, each with their own following. Recently, companies have sought mapping from one framework to another but this is imperfect solution because some frameworks are exclusively technical control measures with very …

Effectiveness Of Drafting Models For Engineering Technology Students And Impacts On Spatial Visualization Ability: An Analysis And Consideration Of Critical Variables, Petros J. Katsioloudis, Jill E. Stefaniak

STEMPS Faculty Publications

Results from a number of studies indicate that the use of drafting models can positively influence the spatial visualization ability for engineering technology students. However, additional variables such as light, temperature, motion and color can play an important role but research provides inconsistent results. Considering this, a set of 5 quasi-experimental studies, was conducted to identify additional critical variables. According to the results, a dynamic, 3Dprinted drafting model, presented with a blue background under lighting conditions between 500–750 lux had the highest impact on spatial visualization ability of engineering technology students.

Investigation Of Ribbed Dome Including Tuned Mass Damper, Ali Parva

Civil & Environmental Engineering Master's Projects

This research report presents the outcome of an investigation of lightweight ribbed steel dome structure. The proposed ribbed dome structure is studied both numerically and experimentally under static, natural vibration, and base excitation conditions. The base excitation is considered in the form of a sinusoidal as well as seismic loading in the form of a scaled-down 1940 El Centro earthquake. A numerical study of the ribbed dome is also conducted including a tuned mass damper (TMD). The performance of the dome is also compared with that of a hexagonal vertical structure showing a superior response of the dome under seismic …

A Purification And In Vitro Activity Assay For A (P)Ppgpp Synthetase From Clostridium Difficile, Astha Pokhrel, Asia Poudel, Erin B. Purcell

Chemistry & Biochemistry Faculty Publications

Kinase and pyrophosphokinase enzymes transfer the gamma phosphate or the beta-gamma pyrophosphate moiety from nucleotide triphosphate precursors to substrates to create phosphorylated products. The use of γ-³²-P labeled NTP precursors allows simultaneous monitoring of substrate utilization and product formation by radiography. Thin layer chromatography (TLC) on cellulose plates allows rapid separation and sensitive quantification of substrate and product. We present a method for utilizing the thin-layer chromatography to assay the pyrophosphokinase activity of a purified (p)ppGpp synthetase. This method has previously been used to characterize the activity of cyclic nucleotide and dinucleotide synthetases and is broadly suitable for …

A Numerical Study On Surface Wave Evolution Over Viscoelastic Mud, Elham Sharifineyestani, Navid Tahvildari

Civil & Environmental Engineering Faculty Publications

A numerical modeling approach is applied to investigate the combined effect of wave-current-mud on the evolution of nonlinear waves. A frequency-domain phase-resolving wave-current model that solves nonlinear wave-wave interactions is used to solve wave evolution. A comparison between the results of numerical wave model and the laboratory experiments confirms the accuracy of the numerical model. The model is then applied to consider the effect of mud properties on nonlinear surface wave evolution. It is shown that resonance effect in viscoelastic mud creates a complex frequency-dependent dissipation pattern. In fact, due to the resonance effect, higher surface wave frequencies can experience …

Driving Factors On Runoff Change In The North Semiarid Rapidly Urbanization Watershed, Yong Bai, Rui-Zhong Gao, Xi-Xi Wang, Wei-Na Wang

Civil & Environmental Engineering Faculty Publications

The hydrological process of a watershed influences a lot on the local socioeconomic development. The analysis of runoff change caused by the rapid urbanization on the watershed is of important significance in the management and protection of the local water resource. In this study, based on the land use information from remotely sensed images, the hydrometeorological observation data and the social economy data from 1997 to 2015, the relationship between the influencing factors and the runoff trend in Wulanmulun River watershed was analyzed at different time scale using the improved Mann-Kendall Test and PCA (principal component analysis) method. The results …

Plasma Medicine: A Brief Introduction, Mounir Laroussi

Electrical & Computer Engineering Faculty Publications

This mini review is to introduce the readers of Plasma to the field of plasma medicine. This is a multidisciplinary field of research at the intersection of physics, engineering, biology and medicine. Plasma medicine is only about two decades old, but the research community active in this emerging field has grown tremendously in the last few years. Today, research is being conducted on a number of applications including wound healing and cancer treatment. Although a lot of knowledge has been created and our understanding of the fundamental mechanisms that play important roles in the interaction between low temperature plasma and …

Novel Contact Materials For Improved Performance Cdte Solar Cells, Angus Rocket, Robert Collins, Sylvain Marsillac

Electrical & Computer Engineering Faculty Publications

This program has explored a number of novel materials for contacts to CdTe solar cells in order to reduce the back contact Schottky barrier to zero and produce an ohmic contact. The project tested a wide range of potential contact materials including TiN, ZrN, CuInSe₂:N, a-Si:H and alloys with C, and FeS₂. Improved contacts were achieved with FeS₂. As part of understanding the operation of the devices and controlling the deposition processes, a number of other important results were obtained. In the process of this project and following its conclusion it led to research …