Engineering Commons^™

Comparison Of Electrically Mediated And Liposome-Complexed Plasmid Dna Delivery To The Skin, Loree C. Heller, Mark J. Jaroszeski, Domenico Coppola, Richard Heller

Bioelectrics Publications

BACKGROUND: Electroporation is an established technique for enhancing plasmid delivery to many tissues in vivo, including the skin. We have previously demonstrated efficient delivery of plasmid DNA to the skin utilizing a custom-built four-plate electrode. The experiments described here further evaluate cutaneous plasmid delivery using in vivo electroporation. Plasmid expression levels are compared to those after liposome mediated delivery.

METHODS: Enhanced electrically-mediated delivery, and less extensively, liposome complexed delivery, of a plasmid encoding the reporter luciferase was tested in rodent skin. Expression kinetics and tissue damage were explored as well as testing in a second rodent model.

RESULTS: Experiments …

Parameter Estimation Of The Arterial System, James Charles Carter Ii

Mechanical & Aerospace Engineering Theses & Dissertations

There are a number of disorders that originate from or involve faulty operation of the cardiovascular system. Diseases such as atherosclerosis, diabetes and hypertension can have a debilitating effect on blood flow. This makes the tools for simulating the effects of such diseases on blood flow important. Measures, such as pulse wave velocity, that are generated by models of the cardiovascular system can be important indicators of cardiac health. Although physically measurable, obtaining some parameters comes with a high cost and discomfort to the patient. Models can provide an assessment of many important parameters. The purpose of this project was …

Using Timed-Release Cryptography To Mitigate Preservation Risk Of Embargo Periods, Rabia Haq

Computer Science Theses & Dissertations

This research defines Time-Locked Embargo, a framework designed to mitigate the Preservation Risk Interval: the preservation risk associated with embargoed scholarly material. Due to temporary access restrictions, embargoed data cannot be distributed freely and thus preserved via data refreshing during the embargo time interval. A solution to mitigate the risk of data loss has been developed by suggesting a data dissemination framework that allows data refreshing of encrypted instances of embargoed content in an open, unrestricted scholarly community. This framework has been developed by exploiting implementations of existing technologies to"time-lock" data using Timed-Release Cryptology (TRC) so that it can be …

Engineering Management Framework In Support Of Modeling & Simulation Application For Domain Specific Procurement, Thomas Guy Litwin

Engineering Management & Systems Engineering Theses & Dissertations

A strategic process is desirable for project-based organizations in order for them to be efficient and effective when developing Modeling & Simulation (M&S) systems. This thesis proposes an overarching process that combines traditional M&S and Engineering Management methodologies in a new framework to support M&S organizations during the procurement process.

This thesis proposes both a Strategic Project Management Process (SPMP) and a systems engineering process for M&S federation development projects. The systems engineering process utilizes the artifacts of Model Driven Architecture (MDA) to support building M&S federations driven by operational requirements. Detailed research of this systems engineering process revealed a …

Modeling Geographic Population Dispersion Utilizing Geospatial Information System Environment Agents In An Agent Based Model, Karl D. Liebert

Computational Modeling & Simulation Engineering Theses & Dissertations

New capabilities in agent based model (ABM) environments are opening the door to the creation of ABM that may incorporate high-quality, real world environment data in the form of Geospatial Information System (GIS) files . In the past, GJS environments were only possible through extensive software development efforts focused on specific model applications.

This thesis explores the value and issues of using GIS data to provide an ABM environment. It examines the ability of ABM development environments (DE) to support GIS environment data. The selected ABM DE is then used to model population behavior in an environment defined by authoritative …

Modeling And Stability Analysis Of Nonlinear Sampled-Data Systems With Embedded Recovery Algorithms, Heber Herencia-Zapana

Electrical & Computer Engineering Theses & Dissertations

Computer control systems for safety critical systems are designed to be fault tolerant and reliable, however, soft errors triggered by harsh environments can affect the performance of these control systems. The soft errors of interest which occur randomly, are nondestructive and introduce a failure that lasts a random duration. To minimize the effect of these errors, safety critical systems with error recovery mechanisms are being investigated. The main goals of this dissertation are to develop modeling and analysis tools for sampled-data control systems that are implemented with such error recovery mechanisms. First, the mathematical model and the well-posedness of the …

Reflection High-Energy Electron Diffraction Studies Of Indium Phosphide (100) And Growth On Indium And Indium Nitride On Silicon (100), Mohamed Abd-Elsattar Hafez

Electrical & Computer Engineering Theses & Dissertations

Study of the effects of atomic hydrogen exposure on structure and morphology of semiconductor surfaces is important for fundamental properties and applications. In this dissertation, the electron yield of a hydrogen-cleaned indium phosphide (InP) surface was measured and correlated to the development of the surface morphology, which was monitored by in situ reflection high-energy electron diffraction (RHEED). Atomic hydrogen treatment produced a clean, well-ordered, and (2x4)-reconstructed InP(100) surface. The quantum efficiency, after activation to negative electron affinity, and the secondary electron emission were shown to increase with hydrogen cleaning time. RHEED patterns of low-index InP(100) surface were modified by the …

Using Agile Software Development Practices In A Research Oriented Distributed Simulation, Douglas James Mielke

Computational Modeling & Simulation Engineering Theses & Dissertations

Although sometimes controversial, agile methodologies have proven to be a viable choice for some software development projects. Projects suited to agile methodologies are those that involve new technology, have requirements that change rapidly, and are controlled by small, talented teams. Much literature about agile software development leans towards business products and non-government entities. Only a handful of literature resources mention agile software development being used in government contracts and even fewer resources mention research projects. NASA's Airspace and Traffic Operations Simulation (ATOS) is a research oriented simulation that doesn't follow the traditional business project mold. In an effort to gain …

Biology-Inspired Approach For Communal Behavior In Massively Deployed Sensor Networks, Kennie H. Jones

Computer Science Theses & Dissertations

Research in wireless sensor networks has accelerated rapidly in recent years. The promise of ubiquitous control of the physical environment opens the way for new applications that will redefine the way we live and work. Due to the small size and low cost of sensor devices, visionaries promise smart systems enabled by deployment of massive numbers of sensors working in concert. To date, most of the research effort has concentrated on forming ad hoc networks under centralized control, which is not scalable to massive deployments. This thesis proposes an alternative approach based on models inspired by biological systems and reports …

Integrating Preservation Functions Into The Web Server, Joan A. Smith

Computer Science Theses & Dissertations

Digital preservation of theWorldWideWeb poses unique challenges, different fromthe preservation issues facing professional Digital Libraries. The complete list of a website’s resources cannot be cited with confidence, and the descriptive metadata available for the resources is so minimal that it is sometimes insufficient for a browser to recognize. In short, the Web suffers from a counting problem and a representation problem. Refreshing the bits, migrating from an obsolete file format to a newer format, and other classic digital preservation problems also affect the Web. As digital collections devise solutions to these problems, the Web will also benefit. But the core …

Caffeine Model Identification For Vigilance Performance Prediction, Chun-Hui Huang

Mechanical & Aerospace Engineering Theses & Dissertations

The pharmacodynamics and pharmacokinetics of caffeine have been well characterized. In this study, a caffeine dynamic model is developed to describe its pharmacodynamic effects on vigilance performance. Validated biomathematical models developed to address both individual and group fatigue and alertness in a non-laboratory setting represent a tremendous commercial opportunity. First, a test data set with caffeine effects isolated from circadian and homeostatic effects is created. Then a modeling approach for input and output effects is developed and different model structures for the caffeine effects are considered. Observer/Kalman filter Identification (OKID) algorithm is proposed and developed to identify the caffeine model …

Real-Time Ultrasound Simulation For Medical Training And Standardized Patient Assessment, Bo Sun

Computational Modeling & Simulation Engineering Theses & Dissertations

With the increasing role played by ultrasound in clinical diagnostics, ultrasound training in medical education has become more and more important. The clinical routine for ultrasound training is on real patients; therefore monitored and guided examinations involving medical students are quite time-constrained. Furthermore, standardized patients (SPs), who are increasingly used in medical school for teaching and assessing medical students, need to be augmented. These SPs are typically healthy individuals who can not accurately portray the variety of abnormalities that are needed for training especially when medical examinations involve instrument interactions. To augment SPs in a realistically effective way and also …

The Application Of Innovative High-Throughput Techniques To Serum Biomarker Discovery, Izabela Debkiewicz Karbassi

Theses and Dissertations in Biomedical Sciences

Time-of-flight mass spectrometry continues to evolve as a promising technique for serum protein expression profiling and biomarker discovery. As seen in our initial SELDI-TOF MS and MALDI-TOF MS profiling study of serum for the assessment of breast cancer risk, many profiling strategies typically employ single chemical affinity beads or surfaces to decrease sample complexity of dynamic fluids like serum. However, most proteins, captured on a particular surface or bead, are not resolved in the lower mass range where mass spectrometers are most effective. To this end we have designed an expression profiling workflow that utilizes immobilized trypsin paramagnetic beads in …

Disassembly Planning And Costing Through Petri Net Approach, Chunhsi Lei

Mechanical & Aerospace Engineering Theses & Dissertations

In the current consumer oriented environment, many new products appear in the market almost on a daily basis. Lured by advertisements and tempted by new product features, customers are constantly purchasing newer products. Acquiring newer products for often leads to throwing out older ones, but it is a totally different story for manufacturers. They need to consider the best way to reuse a product both for economic purposes and for environmental protection. Considerations for them often include: how to minimize total disassembly cost, how to achieve the lowest total disassembly time at each processing step, and how to sort valuable …

Uncertainty Assessment Of Aircraft Maintenance Times By Using Evidence Theory And Expert Judgment Elicitation, Huseyin Kudak

Engineering Management & Systems Engineering Theses & Dissertations

The goal of this study is to demonstrate the use of the Dempster-Shafer Theory of evidence as a decision aid to predict aircraft maintenance times during wartime operations using expert judgment elicitation. Increased precision in time estimation enables the jet engine aircraft maintenance facility commander to make more accurate decisions for the Air Force's wartime tactical operations allowing the commander to gain a decisive advantage. A questionnaire was designed to elicit judgments from experts in the Aircraft Maintenance Facility (AMF) to investigate maintenance times of the major failure modes (Ignition, Fuel, and Electrical). Results of the expert judgment elicitation were …

Evaluation Of The Applicability Of Jc3iedm Based Information Exchange Standardization Efforts To Link-16 Based Systems, Anthony Devivi

Computational Modeling & Simulation Engineering Theses & Dissertations

Command and Control (C2) systems are critical components in decision-making systems in both the military and civilian domains. These systems act as the data evaluation and dissemination tools for larger Command, Control, Computer, Communication, Intelligence, Surveillance, and Recognizance (C4ISR) systems. Typical C2 systems accept a wide variety of inputs from many sources such as radars, weapons systems, simulators, operators, and other C2 systems. The C2 system processes the input data and presents to the user information with which decisions are made. The C2 system also determines what information should be translated and disseminated to other C2 platforms.

The basis for …

Parallel Mpi/Fortran Finite Element Symmetrical/Unsymmetrical Domain Decomposition, Siroj Tungkatara

Civil & Environmental Engineering Theses & Dissertations

MPI/FORTRAN finite element analysis software based on Domain Decomposition (DD) formulas has been developed in this work. Efficient input data storage/data communication schemes, domain partitioning, fast symbolical and numerical sparse assembly, symmetrical/unsymmetrical sparse solver and robust symmetrical/unsymmetrical iterative solvers algorithms are all incorporated into the developed code. Parallel Precondition Conjugated Gradient (PCG) and Flexible Generalized Minimum Residual (FGMRES) are developed. Efficient computational techniques used in the developed code are explained. Numerical performance and the accuracy of the developed code are conducted on acoustic examples with medium to large grid sizes. The results obtained from ODU Wilbur cluster (under parallel processing …

Process Study For The Design Of Small-Scale 2 Kelvin Refrigeration Systems, Peter N. Knudsen

Mechanical & Aerospace Engineering Theses & Dissertations

Helium refrigeration at temperatures below 4.5-Kelvin (K), but greater than 0.8-K typically employ a sub-atmospheric process utilizing a vacuum pumping system. These types of helium refrigerators are of keen interest to present and future particle physics programs utilizing super-conducting magnet or radio-frequency technology. As such, there is a need for small scale 2-K helium refrigeration systems (i.e., those that operated below the lambda temperature) in small laboratories and test facilities at this time. This study establishes the key process parameter choices of flow ratio, heat-exchanger size, and supply pressure, and how they influence the overall system performance for various process …

Developing A Repeatable And Reliable Methodology To Determine Return-On-Investment, Mark Cullen Nesselrode

Computational Modeling & Simulation Engineering Theses & Dissertations

The high costs of modern weapons systems, fuel, personnel, and increasing environmental awareness is forcing U.S. forces to rely on simulation for training. Further, the need to train in a robust and theater specific environment is critical to ensure operational readiness immediately upon reaching the combat theater. Finally, the rapidly changing nature of the conflicts, crises, or contingencies in which U.S. forces are involved requires a new approach to training.

The purpose of this research is to develop and demonstrate the feasibility of a methodology that can provide a reliable, consistent method for determining ROI in various forms, focusing on …

Kilovolt Blumlein Pulse Generator With Variable Pulse Duration And Polarity, Andrea De Angelis, Juergen F. Kolb, Luigi Zeni, Karl H. Schoenbach

Bioelectrics Publications

A Blumlein pulse generator which utilizes the superposition of electrical pulses launched from two individually switched pulse forming lines has been designed and tested. By using a power metal-oxide-semiconductor field-effect transistor as a switch on each end of the Blumlein line, we were able to generate pulses with amplitudes of 1kV across a 100Ω load. Pulse duration and polarity can be controlled by the temporal delay in the triggering of the two switches. Using this technique, we have demonstrated the generation of pulses with durations between 8 and 60ns. The lower limit in pulse duration was determined by the switch …

The Effect Of Stirring On The Morphology Of Birnessite Nanoparticles, Marcos A. Cheney, Pradip K. Bhowmik, Shingo Moriuchi, Mario Villabos, Shizhi Qian, Sang W. Joo

Mechanical & Aerospace Engineering Faculty Publications

The effect of mechanical stirring on the morphology of hexagonal layer- structure birnessite nanoparticles produced from decomposition of KMnO₄ in dilute aqueous H₂SO₄ is investigated, with characterization by X- ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high- resolution transmission electron microscopy (HRTEM), thermogravimetric analysis (TGA), and N₂ adsorption (BET). Mechanical stirring during an initial stage of synthesis is shown to produce black birnessite containing nanofibers, whereas granular particulates of brown birnessite are produced without stirring. This is the first reduction synthesis of black birnessite nanoparticles with dendritic morphology without any use …

Development And Validation Of A Dynamic Model Of The Maglev Transportation System At Old Dominion University, Ajinkya Deodhar, Sebastian Bawab, Aravind Hanasoge

Mechanical & Aerospace Engineering Faculty Publications

A dynamic model of the vehicle/guideway coupled with a controller is developed for the maglev demonstration system currently being developed at ODU, using the MAthematical DYnamic MOdeling software - MADYMO. The fundamental characteristics of the vehicle and guideway are obtained from detailed finite element analyses using MSC-NASTRAN. As a result, the vehicle is modeled in MADYMO as a 21- degree-of-freedom spring-mass-damper system. A three span concrete guideway is modeled using 3D solid Hex8 elements. The air gap is modeled as a penetration of the magnets into the guideway. Decentralized colocated PD controllers are used for controlling the penetration of each …

Emergent Behavior In Massively-Deployed Sensor Networks, Ekaterina Shurkova, Ruzana Ishak, Stephan Olariu, Shaharuddin Salleh

Computer Science Faculty Publications

The phenomenal advances in MEMS and nanotechnology make it feasible to build small devices, referred to as sensors that are able to sense, compute and communicate over small distances. The massive deployment of these small devices raises the fascinating question of whether or not the sensors, as a collectivity, will display emergent behavior, just as living organisms do. In this work we report on a recent effort intended to observe emerging behavior of large groups of sensor nodes, like living cells demonstrate. Imagine a massive deployment of sensors that can be in two states "red" and "blue". At deployment time …

Creating Preservation-Ready Web Resources, Joan A. Smith, Michael L. Nelson

Computer Science Faculty Publications

There are innumerable departmental, community, and personal web sites worthy of long-term preservation but proportionally fewer archivists available to properly prepare and process such sites. We propose a simple model for such everyday web sites which takes advantage of the web server itself to help prepare the site's resources for preservation. This is accomplished by having metadata utilities analyze the resource at the time of dissemination. The web server responds to the archiving repository crawler by sending both the resource and the just-in-time generated metadata as a straight-forward XML-formatted response. We call this complex object (resource + metadata) a CRATE. …

Reproducible Increased Mg Incorporation And Large Hole Concentration In Gan Using Metal Modulated Epitaxy, Shawn D. Burnham, Gon Namkoong, David C. Look, Bruce Clafin, W. Alan Doolittle

Electrical & Computer Engineering Faculty Publications

The metal modulated epitaxy (MME) growth technique is reported as a reliable approach to obtain reproducible large hole concentrations in Mg-doped GaN grown by plasma-assisted molecular-beam epitaxy on c-plane sapphire substrates. An extremely Ga-rich flux was used, and modulated with the Mg source according to the MME growth technique. The shutter modulation approach of the MME technique allows optimal Mg surface coverage to build between MME cycles and Mg to incorporate at efficient levels in GaN films. The maximum sustained concentration of Mg obtained in GaN films using the MME technique was above 7 × 10²⁰ cm^{-3 …}

Seasonal Adaptation Of Vegetation Color In Satellite Images, Srinivas Jakkula, Vamsi K.R. Mantena, Ramu Pedada, Yuzhong Shen, Jiang Li, Hamid R. Arabnia (Ed.)

Electrical & Computer Engineering Faculty Publications

Remote sensing techniques like NDVI (Normal Difference vegetative Index) when applied to phenological variations in aerial images, ascertained the seasonal rise and decline of photosynthetic activity in different seasons, resulting in different color tones of vegetation. The rise and fall of NDVI values decide the biological response, either the green up or brown down [1]. Vegetation in green up period appears with more vegetative vigor and during brown down period it has a dry appearance. This paper proposes a novel method that identifies vegetative patterns in satellite images and then alters vegetation color to simulate seasonal changes based on training …

Vegetation Identification Based On Satellite Imagery, Vamsi K.R. Mantena, Ramu Pedada, Srinivas Jakkula, Yuzhong Shen, Jiang Li, Hamid R. Arabnia (Ed.)

Electrical & Computer Engineering Faculty Publications

Automatic vegetation identification plays an important role in many applications including remote sensing and high performance flight simulations. This paper presents a method to automatically identify vegetation based upon satellite imagery. First, we utilize the ISODATA algorithm to cluster pixels in the images where the number of clusters is determined by the algorithm. We then apply morphological operations to the clustered images to smooth the boundaries between clusters and to fill holes inside clusters. After that, we compute six features for each cluster. These six features then go through a feature selection algorithm and three of them are determined to …

Dedication To Pierre Lallemand On The Occasion Of His Retirement, Dominque D'Humieres, Manfred Krafczyk, Li-Shi Luo, Robert Rubinstein

Mathematics & Statistics Faculty Publications

The fourth international conference for mesoscopic methods in engineering and science (http://www.icmmes. org), held in Munich, Germany, 16–20 July 2007, was closed with a celebration honouring Dr Pierre Lallemand on the occasion of his retirement from the Centre National de la Recherche Scientifique (CNRS) after more than 40 years of service.

Efficient Corona Training Protocols For Sensor Networks, Alan A. Bertossi, Stephan Olariu, Cristina M. Pinotti

Computer Science Faculty Publications

Phenomenal advances in nano-technology and packaging have made it possible to develop miniaturized low-power devices that integrate sensing, special-purpose computing, and wireless communications capabilities. It is expected that these small devices, referred to as sensors, will be mass-produced and deployed, making their production cost negligible. Due to their small form factor and modest non-renewable energy budget, individual sensors are not expected to be GPS-enabled. Moreover, in most applications, exact geographic location is not necessary, and all that the individual sensors need is a coarse-grain location awareness. The task of acquiring such a coarse-grain location awareness is referred to as training. …

Community-Of-Interest (Coi) Model-Based Languages Enabling Composable Net-Centric Services, Andreas Tolk, Saikou Y. Diallo

Computational Modeling & Simulation Engineering Faculty Publications

Net-centric services shall be designed to collaborate with other services used within the supported Community of Interest (COI). This requires that such services not only be integratable on the technical level and interoperable on the implementation level, but also that they are composable in the sense that they are semantically and pragmatically consistent and able to exchange information in a consistent and unambiguous way.

In order to support Command-and-Control with Composable Net-centric Services, the human-machine interoperation must be supported as well as the machine-machine interoperation. This paper shows that techniques of computer linguistic can support the human-machine interface by structuring …