Biological Engineering Commons^™

Advancing Biofabrication Methodologies To Develop Biomimetic Tissue Scaffolds For Musculoskeletal Applications, Nashaita Yezdi Patrawalla

Theses and Dissertations

Musculoskeletal injuries impose a significant global burden, affecting millions and generating an economic toll exceeding $14 billion. Tissue engineering has gained traction as a promising strategy offering potential for functional restoration of damaged musculoskeletal tissues. This research aims to advance biofabrication techniques for generation of biomimetic tissue scaffolds, by optimizing scaffold design and modulating fabrication process parameters to generate scaffolds for musculoskeletal regeneration with application-specific tailored properties. In this realm, collagen-based biomaterials have been extensively investigated for tendon and ligamentous applications, and in combination with bioceramics incorporated into the collagen framework to produce composite scaffolds that better recapitulate the native …

Investigating The Role Of Cd44 In Her2+ Breast Cancer Cells During Cancer Cell Redirection, Holly Caroline Jordan Campbell

All Dissertations

Despite a rise in survival rates of breast cancer patients due to advances in modern medicine, breast cancer is the most common type of cancer in women and is the second leading cause of cancer death in the United States. Early detection and a more personalized treatment of breast cancer has slowly improved patient outcomes. Nevertheless, there are impediments to better patient outcomes due to the complexity of the disease physiology. Therefore, there is a demand for a comprehensive understanding of cancer development as well as a need to understand tumor progression for enhanced therapies for patients.

Previous in vivo …

Analysis Of An Outdoor Pilot-Scale Rotating Algae Biofilm Reactor For Power Optimization, Ash-Enhanced Productivity, And Nutrient Uptake, Peter F. Jeppesen

All Graduate Theses and Dissertations, Fall 2023 to Present

Outdoor testing of a rotating algae biofilm reactor (RABR) was performed to see how well it could remove nutrients from water and produce algae. By adjusting the operation settings, we found the lowest power needed to remove nitrogen and phosphorus. The lowest power values needed were 1.36 kWh per kilogram of nitrogen and 20.1 kWh per kilogram of phosphorus. The algae production rate reached a maximum of 7.09 grams per square meter per day. Detailed analysis showed that the reactor worked like a continuous flow system, and the performance was influenced by factors including light and temperature. The results also …

Engineered Microalgae Cultivations Systems: Conversion Of Wastewater Nutrients Into Biofuels And Bioplastics, Jacob Watkins

All Graduate Theses and Dissertations, Fall 2023 to Present

Dissolved Nitrogen and Phosphorus in wastewater can contribute to harmful algae blooms if released into the environment. One technology that can be used to recover dissolved nutrients from wastewater is the Rotating Algae Biofilm Reactor (RABR), which supports microalgae growth in an easily-harvested biofilm and produces nitrogenand phosphorus-rich biomass that can be used to produce slow-release fertilizers, biofuels, and compostable bioplastics. This thesis (1) examines the effects of several environmental factors on the biomass production rate and nutrient removal efficiency of RABRs treating municipal wastewater, (2) quantifies the composition and biofuel yields of microalgae biomass cultivated using a RABR at …

The Impact Of Cumulative And Acute Loading On Achilles Tendon Health, Performance, And Reported Pain In Collegiate Gymnasts, Julio Serrano Samayoa

Electronic Theses and Dissertations

Gymnasts are at a high risk of Achilles tendon (AT) injuries due to intense loads during takeoffs and landings. To better understand better the relationship between impact loading and its effects on tendon adaptation and athletic performance, this study uses Inertial Measurement Units (IMUs) to track AT loading across a collegiate gymnastics season. This data were compared against physiological and athletic performance metrics, obtained through force plates and ultrasound imaging, to explore how mechanical load influences reported pain and athletic performance. Results varied, with cumulative impacts being correlated with improved jump height (Spearman 0.362, Pearson 0.348), while peak power and …

Hypoxic Incubator: Improving Robustness/Reliability And Demonstrating Physiological Efficacy, Damon Dennis Tan

Master's Theses

The Microphysiological Systems Laboratory aims to develop colorectal cancer tumor models under a hypoxic environment to assess model response to pharmaceutical compounds in vitro. To perform relevant studies, researchers have attempted to use different hypoxic inducing strategies such as a nitrogen pod and hypoxic incubator to recreate in vivo physiological responses to hypoxia. However, studies would be interrupted due to incubator functionality failure. To ensure successful and physiologically relevant studies, I improved and verified the robustness and reliability of a hypoxic incubator previously designed and manufactured in the lab. Through the testing and iterating design processes, I engineered and implemented …

3d Printed Microfluidic Fabrication Methodology, Characterization, Mechanical Design, And Applications In Electrostatic Artificial Muscles And Benthic Microbial Fuel Cells, Terak B. Hornik

Master's Theses

The fabrication of microfluidic devices often requires specialized methods. The development of these methods requires careful characterization and understanding of the processes involved. Using primarily PolyJet 3D printing technology, microfluidics offers a wide scope of applications such as microfluidic benthic microbial fuel cells (MBMFCs) and electrostatic artificial muscles. MBMFCs benefit from the confinement of the microbes resulting in close proximity between the electrode and the organisms. Using a modular design called the Sponge, assembly and upscaling is possible. Electrostatic artificial muscles benefit from a microfluidic approach due to the non-linearity of electrostatic attraction creating disproportionate benefits when miniaturized. When designed …

Microwave Catalysis-Enabled Membrane Processes For Microbial Inactivation, Fangzhou Liu

Dissertations

The COVID-19 pandemic has highlighted the urgent need for advanced technologies to combat virus transmission. Traditional membrane filtration technologies primarily serve as physical barriers, capturing pollutants but failing to inactivate microbial agents such as bacteria and viruses. These membranes typically target larger particles but are ineffective against sub-micrometer viral particles. Thus, there's a critical demand for membrane filtration processes that combine robust filtration with antifouling and reaction -enabled functions for efficient pathogen disinfection. This research explores a novel microwave-assisted membrane filtration process incorporating catalytic reactions to enhance microbial disinfection and prevent membrane fouling, offering a groundbreaking solution to global challenges …

Sustainable Waste-To-Bioproducts Engineering Center Laboratory Safety Project, Claudine Mock

All Graduate Reports and Creative Projects, Fall 2023 to Present

Laboratory accidents are common in teaching and research labs; these accidents can be injurious or even fatal. The consequences of each type of laboratory accident are different based on the activities performed and the hazardous materials used in each facility. However, the most common issue related to laboratory accidents is the lack of proper safety measures (e.g., poor handling of hazardous materials, not reporting incidents immediately, etc.). This project aimed to establish and strengthen best practices and procedures to ensure laboratory safety at the Utah State University Sustainable Waste-to-Bioproducts Engineering Center. To achieve this goal, different practices and measures were …

The Role Of Kinases Signaling In Organogenesis, Fatemeh Nasehi

All Dissertations

Kinases are crucial regulators of organ development and differentiation. This thesis investi- gates the roles of TAK1, TGFbeta-Activate Kinase 1 (TAK1/Map3K7), and Akt3 kinases in skeletal, cardiac muscle, and cartilage. TAK1 is pivotal for the differentiation of various organ systems, including sinoatrial node and cartilage, and is responsible for phosphorylat- ing a diverse array of downstream kinases. We identified several novel proteins, including phospho-Akts, that are upregulated in response to TAK1 activation. We discovered severe Dilated Cardiomyopathy (DCM) and cartilage defects in Akt3 mutant mice. Although this mouse mutant has been studied for over two decades, we are the first …

Engineered Nucleic-Acid Binding Intrinsically Disordered Proteins For Biomedical Applications, Telmo Diez Perez

Biomedical Engineering ETDs

This dissertation explores the use of intrinsically disorder proteins (IDPs) in biomedical applications related to the detection and biosensing of nucleic-acid species. We engineer several low critical solution temperature (LCST) nucleic-acid binding elastin-like polypeptides (ELPs) as model IDPs and study their potential in biomedical application via interactions with short single-stranded (ss) DNA, tRNA and viral RNAs. First, we engineer a simple polycationic ELP capable of recruiting DNA on demand in a DNA-protein-rich condensate via LLPS. Moreover, we develop a modified Flory-Huggins theory to quantify the partitioning of DNA and protein upon coacervate formation with and without salts. We then design …

Feasibility Study For The Application Of A Solar Powered Absorption Chilling System For Cooling Poultry Houses In Northwest Arkansas, Matthew James Kelly

Graduate Theses and Dissertations

This paper investigates the economic feasibility of utilizing a solar-powered absorption chiller to provide cooling for poultry houses in northwest Arkansas. An existing thermodynamic model of a poultry house was modified to account for cooling. A simulation was performed using actual weather data from Fayetteville, Arkansas for 26 flocks of broilers at four cooling capacities for the 2020, 2021, and 2022 calendar years. Based on the simulation, the savings from reduced electricity use for ventilation and the cost of the required absorption chiller and solar thermal array for an 8-house farm were calculated. While providing cooling was found to reduce …

Optimization Of And Mechanistic Insights Into Photocatalytic And Non-Thermal Plasma Degradation Of Organic Pollutants For Sustainable Wastewater Treatment, Yiting Xiao

Graduate Theses and Dissertations

Water pollution by organic pollutants poses a significant environmental threat, necessitating the development of advanced treatment technologies to ensure sustainable and efficient remediation. This dissertation explored innovative approaches to enhance the degradation of organic pollutants through the integration of photocatalytic and plasma-assisted systems, with a focus on optimizing these processes using methodologies ranging from response surface methodology (RSM) to advanced machine learning techniques. The first part of the dissertation investigated the degradation of organic pollutants in flocculated liquid digestate using photocatalytic titanate nanofibers (TNFs) synthesized via a hydrothermal method. This study represents the first application of TNFs, with a bandgap …

Shape Memory Alloy Capsule Micropump For Drug Delivery Applications, Youssef Mohamed Kotb

Theses and Dissertations

Implantable drug delivery devices have many benefits over traditional drug administration techniques and have attracted a lot of attention in recent years. By delivering the medication directly to the tissue, they enable the use of larger localized concentrations, enhancing the efficacy of the treatment. Passive-release drug delivery systems, one of the various ways to provide medication, are great inventions. However, they cannot dispense the medication on demand since they are nonprogrammable. Therefore, active actuators are more advantageous in delivery applications. Smart material actuators, however, have greatly increased in popularity for manufacturing wearable and implantable micropumps due to their high energy …

Statistical Modeling Of Knee Morphology And Material Properties Considering Diverse Populations, Gabrielle Jeannine Kindy

Electronic Theses and Dissertations

Total Knee Arthroplasty (TKA) is a widely performed surgical procedure aimed at alleviating pain and restoring function in patients with severe knee osteoarthritis. Despite its general success, disparities in postoperative outcomes have been observed across different racial and ethnic groups, with minority populations often experiencing less favorable results. One potential avenue for improving the generalizability of orthopaedic implants is using Statistical Shape and Intensity Models (SSIMs), which can be used to incorporate patient variability directly into the orthopaedic medical device development workflow through population-based finite element analysis.

This work aimed to construct an SSIM from a diverse subject set, incorporating …

Calcium Analysis Of Intercellular Communications Between Alpha Cells And Delta Cells Across The Pancreatic Islet, Shichao Gao

McKelvey School of Engineering Graduate Student Theses & Dissertations

The intricate communication between different cell types within pancreatic islets plays a crucial role in regulating glucose homeostasis. Among these cellular interactions, the interplay between α cells (responsible for glucagon secretion) and δ cells (secreting somatostatin) is particularly intriguing due to the inhibitory effects of somatostatin on glucagon release. Understanding the intercellular interactions between α and δ cells may provide insights into the mechanisms underlying the coordinated regulation of blood glucose levels. In this study, we employed a multi-modal approach combining confocal imaging, image analysis, and correlation network analysis to investigate the spatial relationships and potential functional connections between α …

Creating A Collapsible Sling Lift Capable Of Transporting Patients Between Various Locations, Cameron Delaney Hillman, Macey Jewell Ross, Lydia Noel Miller

Honors Theses

As Senior Biomedical Engineering students at the University of Mississippi, we, Lainey Hillman, Lydia Miller, and Macey Ross, are required to complete a final senior capstone for our department. This project aims to provide a solution to a current unmet clinical need in the healthcare industry, thus our team sought to improve the lack of transportability of sling lifts. The current sling lifts on the market display numerous issues: heavy, bulky, use confined to certain areas, and use specific for only one type of transportation. To combat these problems, we designed a sling lift that was lightweight, collapsible, safe, and …

Nodes For Modes: Nodal Honeycomb Metamaterial Enables A Soft Robot With Multimodal Locomotion, Yusuf Dikici, Kathryn Daltorio, Ozan Akkus

Faculty Scholarship

Soft-bodied animals, such as worms and snakes, use many muscles in different ways to traverse unstructured environments and inspire tools for accessing confined spaces. They demonstrate versatility of locomotion which is essential for adaptation to changing terrain conditions. However, replicating such versatility in untethered soft-bodied robots with multimodal locomotion capabilities have been challenging due to complex fabrication processes and limitations of soft body structures to accommodate hardware such as actuators, batteries and circuit boards. Here, we present MetaCrawler, a 3D printed metamaterial soft robot designed for multimodal and omnidirectional locomotion. Our design approach facilitated an easy fabrication process through a …

Analysis And Evaluation Of Different Transformer Architectures For The Protein Sequence Representation And The Corresponding Hypothetical Applications, Mary Mao

Theses and Dissertations

This project is to investigate and assess several Transformer topologies for the modeling of protein sequences and their corresponding uses. Each major model for the protein sequence representations is inspected with its mathematical theory and analyzed for the different performance of the models with various validation repositories.

Controlled Delivery Of Peptide And Gasotransmitter For Promoting Vascular Endothelialization, Shirin Changizi

Theses and Dissertations

Endothelial cells (ECs) are vital for maintaining proper vascular function and regulating processes such as hemostasis and inflammation. Disruption of endothelial cells integrity can lead to several pathological conditions, including coronary artery disease, vascular cognitive impairment, and dementia (VCID). Alterations in ECs distribution may lead to compromised blood-brain barrier (BBB) function, facilitating the passage of blood components and contributing to conditions such as Alzheimer's disease. Additionally, disruptions in ECs function can lead to early-stage thrombosis and intimal hyperplasia, resulting in graft failure in small-diameter (

This study aimed to develop innovative approaches for drug delivery to the BBB and TEVGs …