Hepatoprotective Effect Of The Ethanol Extract Of Polygonum Orientale On Carbon Tetrachloride-Induced Acute Liver Injury In Mice,
2020
Taiwan Food and Drug Administration
Hepatoprotective Effect Of The Ethanol Extract Of Polygonum Orientale On Carbon Tetrachloride-Induced Acute Liver Injury In Mice, Y.-J. Chiu, S.-C. Chou, C.-S. Chiu, C.-P. Kao, K.-C. Wu, C.-J. Chen, J.-C. Tsai, W.-H. Peng
Journal of Food and Drug Analysis
Polygonum orientale L. (Polygonaceae) fruits have various medicinal uses, but their hepatoprotective effects have not yet been studied. This study investigated the hepatoprotective activity of the ethanolic extract of P. orientale (POE) fruits against carbon tetrachloride (CCl 4 )-induced acute liver injury (ALI). Mice were pretreated with POE (0.1, 0.5, and 1.0 g/kg) or silymarin (0.2 g/kg) for 5 consecutive days and administered a dose of 0.175% CCl 4 (ip) on the 5th day to induce ALI. Blood and liver samples were collected to measure antioxidative activity and cytokines. The bioactive components of POE were identified through high-performance liquid chromatography …
Non-Destructive Profiling Of Volatile Organic Compounds Using Hs-Spme/Gc–Ms And Its Application For The Geographical Discrimination Of White Rice,
2020
Taiwan Food and Drug Administration
Non-Destructive Profiling Of Volatile Organic Compounds Using Hs-Spme/Gc–Ms And Its Application For The Geographical Discrimination Of White Rice, D.K. Lim, C. Mo, D.-K. Lee, N.P. Long, J. Lim, S.W. Kwon
Journal of Food and Drug Analysis
The authenticity determination of white rice is crucial to prevent deceptive origin labeling and dishonest trading. However, a non-destructive and comprehensive method for rapidly discriminating the geographical origins of white rice between countries is still lacking. In the current study, we developed a volatile organic compound based geographical discrimination method using headspace solid-phase microextraction coupled to gas chromatography–mass spectrometry (HS-SPME/GC–MS) to discriminate rice samples from Korea and China. A partial least squares discriminant analysis (PLS-DA) model exhibited a good classification of white rice between Korea and China (accuracy = 0.958, goodness of fit = 0.937, goodness of prediction = 0.831, …
A Randomized, Double-Blind Clinical Study Of The Effects Of Ankascin 568 Plus On Blood Lipid Regulation,
2020
Taiwan Food and Drug Administration
A Randomized, Double-Blind Clinical Study Of The Effects Of Ankascin 568 Plus On Blood Lipid Regulation, S.-F. Liu, Y.-R. Wang, Y.-C. Shen, C.-L. Chen, C.-N. Huang, T.-M. Pan, C.-K. Wang
Journal of Food and Drug Analysis
Hyperlipidemia and inflammation play important roles in the development and progression of atherosclerosis. Atherosclerosis is regarded as an inflammatory response of blood vessels to injury at the start of atherosclerotic plaque formation, which then leads to cardiovascular events. Edible fungi of the Monascus species have been used as traditional Chinese medicines in East Asia for several centuries. The fermented products of Monascus purpureus NTU 568 possess a number of functional secondary metabolites including the anti-inflammatory pigments monascin and ankaflavin. Compounds derived from M. purpureus have been shown to have hypolipidemic effects. We aimed to evaluate the effects of M. purpureus …
Efficacy Of Protein Rich Pearl Powder On Antioxidant Status In A Randomized Placebo-Controlled Trial,
2020
Taiwan Food and Drug Administration
Efficacy Of Protein Rich Pearl Powder On Antioxidant Status In A Randomized Placebo-Controlled Trial, H.-F. Chiu, S.-C. Hsiao, Y.-Y. Lu, Y.-C. Han, Y.-C. Shen, K. Venkatakrishnan, C.-K. Wang
Journal of Food and Drug Analysis
Pearl is one of the well-known traditional Chinese medicine (TCM) prescribed for treating various skin and bone related disorders due to its abundant proteins and mineral contents. The present investigation focused on antioxidation and life span prolonging effects from different extracts of pearl powder. During in vitro studies, various oxidative indices were evaluated, along with lifespan-prolonging effect were checked using wild-type Caenorhabditis elegans. For the clinical trial, 20 healthy middle-aged subjects were recruited and separated into 2 groups as experimental and placebo group, who received 3 g of pearl powder/d (n = 10) and 3 g of placebo/d (n = …
Rapid Characterization Of Metabolites In Soybean Using Ultra High Performance Liquid Chromatography Coupled With Electrospray Ionization Quadrupole Time-Of-Flight Mass Spectrometry (Uplc-Esi-Q-Tof-Ms/Ms) And Screening For Α-Glucosidase Inhibitory And Antioxidant Properties Through Different Solvent Systems,
2020
Taiwan Food and Drug Administration
Rapid Characterization Of Metabolites In Soybean Using Ultra High Performance Liquid Chromatography Coupled With Electrospray Ionization Quadrupole Time-Of-Flight Mass Spectrometry (Uplc-Esi-Q-Tof-Ms/Ms) And Screening For Α-Glucosidase Inhibitory And Antioxidant Properties Through Different Solvent Systems, Y.H. Lee, B. Kim, S.-R. Hwang, K. Kim, J.H. Lee
Journal of Food and Drug Analysis
This work was the first to investigate on the simultaneous characterization of metabolite profiles in soybean using UPLC-ESI-Q-TOF-MS/MS. Twenty two compositions were observed within 14 min from the methanol extract and confirmed as twelve isoflavones of three types and ten soyasaponins (Ab, Af, I–III, αg, βg, βa, γg, and γa). Moreover, the patterns of two chemicals showed considerable differences in seven solvent systems by HPLC analysis and their optimal extraction was achieved by 70% methanol (isoflavone: 4102.69 μg/g; soyasaponin: ten peaks). The second abundant isoflavones were detected in 50% methanol (4054.39 μg/g), followed by 30% methanol, 100% methanol, 10% methanol, …
Detection Of Lithospermate B In Rat Plasma At The Nanogram Level By Lc/Ms In Multi Reaction Monitoring Mode,
2020
Taiwan Food and Drug Administration
Detection Of Lithospermate B In Rat Plasma At The Nanogram Level By Lc/Ms In Multi Reaction Monitoring Mode, T.-Y. Chung, N.-H. Lin, Y.-C. Li, T.-Y. Chen, P.-C. Kuo, W.-Y. Chen, J.T.C. Tzen
Journal of Food and Drug Analysis
Low bioavailability and high binding affinity to plasma proteins led to the difficulty for the quantitative detection of lithospermate B (LSB) in plasma. This study aimed to develop a protocol for detecting LSB in plasma. A method was employed to quantitatively detect LSB of 5–500 ng/mL by LC/MS spectrometry in multi reaction monitoring mode via monitoring two major fragments with m/z values of 519 and 321 in the MS2 spectrum. To set up an adequate extraction solution to release LSB captured by plasma proteins, recovery yields of LSB extracted from rat plasma acidified by formic acid or HCl in the …
Study Of Forced Degradation Behavior Of Pramlintide Acetate By Hplc And Lc–Ms,
2020
Taiwan Food and Drug Administration
Study Of Forced Degradation Behavior Of Pramlintide Acetate By Hplc And Lc–Ms, Y. Yuan, Y.-B. Li, Z.-F. Tai, Y.-P. Xie, X.-F. Pu, J. Gao
Journal of Food and Drug Analysis
Pramlintide acetate (Symlin ® ), a synthetic analogue of the human hormone amylin. It was approved in March 2005 as a subcutaneous injection for the adjunctive treatment of patients who have type 1 or 2 diabetes mellitus. The objective of current investigation was to study the degradation behavior of pramlintide acetate under different ICH recommended stress conditions by HPLC and LC–MS. Pramlintide acetate was subjected to stress conditions of hydrolysis (acidic or alkaline), oxidation, photolysis and thermal decomposition. Extensive degradation products were observed under the hydrolysis, oxidation or thermal stress conditions, while minimal degradation was found in the photolytic conditions. …
Methods For Efficient Analysis Of Tocopherols, Tocotrienols And Their Metabolites In Animal Samples With Hplc-Ec,
2020
Taiwan Food and Drug Administration
Methods For Efficient Analysis Of Tocopherols, Tocotrienols And Their Metabolites In Animal Samples With Hplc-Ec, M.-J. Lee, W. Feng, L. Yang, Y.-K. Chen, E. Chi, A. Liu, C.S. Yang
Journal of Food and Drug Analysis
Tocopherols and tocotrienols, collectively known as vitamin E, have received a great deal of attention because of their interesting biological activities. In the present study, we reexamined and improved previous methods of sample preparation and the conditions of high-performance liquid chromatography for more accurate quantification of tocopherols, tocotrienols and their major chain-degradation metabolites. For the analysis of serum tocopherols/tocotrienols, we reconfirmed our method of mixing serum with ethanol followed by hexane extraction. For the analysis of tissue samples, we improved our methods by extracting tocopherols/tocotrienols directly from tissue homogenate with hexane. For the analysis of total amounts (conjugated and unconjugated …
Use Of A Bayesian Approach In The Design And Evaluation Of Nce2s,
2020
Taiwan Food and Drug Administration
Use Of A Bayesian Approach In The Design And Evaluation Of Nce2s, C.-Y. Wang, L.-C. Chang, M.-S. Lin, C.-F. Hsiao, J.-D. Huang
Journal of Food and Drug Analysis
Taiwan's regulatory agency defines New Chemical Entity 2 (NCE2) as a compound drug that has been approved and marketed for ten years in a top-ten pharmaceutically-advanced country but which is new in Taiwan. To apply for registration of NCE2 in Taiwan, a clinical trial may be conducted in Taiwan to evaluate the efficacy and safety. Since the NCE2 has been approved in at least one of the top-ten pharmaceutically-advanced countries, we can borrow the information from all of the observed data from other countries to synthesize the data from both Taiwan and other countries to assess the NCE2 efficacy. In …
Stem Cell Therapy On Skin: Mechanisms, Recent Advances And Drug Reviewing Issues,
2020
Taiwan Food and Drug Administration
Stem Cell Therapy On Skin: Mechanisms, Recent Advances And Drug Reviewing Issues, G.-Y. Chu, Y.-F. Chen, H.-Y. Chen, M.-H. Chan, C.-S. Gau, S.-M. Weng
Journal of Food and Drug Analysis
Stem cell products and its clinical applications have been widely discussed in recent years, particularly when the Japanese “induced pluripotent stem cells” founder Dr. Yamanaka was awarded as Nobel Prize laureate in 2013. For decades, major progresses have been achieved in the stem cell biology field, and more and more evidence showed that skin stem cells are involved in the process of skin repair. Stem/progenitor cells of the epidermis are recognized to play the most essential role in the tissue regeneration of skin. In this review, we first illustrated basic stem cell characteristics and various stem cell subtypes resided in …
Development And Validation Of An Lc–Ms/Ms Method For Quantification Of Nc-8 In Rat Plasma And Its Application To Pharmacokinetic Studies,
2020
Taiwan Food and Drug Administration
Development And Validation Of An Lc–Ms/Ms Method For Quantification Of Nc-8 In Rat Plasma And Its Application To Pharmacokinetic Studies, B.H. Kachingwe, Y.-S. Uang, T.-J. Huang, L.-H. Wang, S.-J. Lin
Journal of Food and Drug Analysis
ent-16-Oxobeyeran-19-N-methylureido (NC-8) is a recently synthesized derivative of isosteviol that showed anti-hepatitis B virus (HBV) activity by disturbing replication and gene expression of the HBV and by inhibiting the host toll-like receptor 2/nuclear factor-κB signaling pathway. To study its pharmacokinetics as a part of the drug development process, a highly sensitive, rapid, and reliable liquid chromatography tandem mass spectrometry (LC–MS/MS) method was developed and validated for determining NC-8 in rat plasma. After protein precipitation extraction, the chromatographic separation of the analyte and internal standard (IS; diclofenac sodium) was performed on a reverse-phase Luna C18 column coupled with a Quattro Ultima …
Studies On Prevention Of Obesity, Metabolic Syndrome, Diabetes, Cardiovascular Diseases And Cancer By Tea,
2020
Taiwan Food and Drug Administration
Studies On Prevention Of Obesity, Metabolic Syndrome, Diabetes, Cardiovascular Diseases And Cancer By Tea, C.S. Yang, H. Wang, Z.P. Sheridan
Journal of Food and Drug Analysis
Tea, a popular beverage made from leaves of the plant Camellia sinensis, has been studied extensively in recent decades for its beneficial health effects in the prevention of obesity, metabolic syndrome, diabetes, cancer, and other diseases. Whereas these beneficial effects have been convincingly demonstrated in most laboratory studies, results from human studies have not been consistent. Some studies demonstrated that weight reduction, alleviation of metabolic syndrome and risk reduction in diabetes were only observed in individuals who consume 3–4 cups of tea (600–900 mg tea catechins) or more daily. This chapter reviews some of these studies, the possible mechanisms of …
A Novel Protein From Edible Fungi Cordyceps Militaris That Induces Apoptosis,
2020
Taiwan Food and Drug Administration
A Novel Protein From Edible Fungi Cordyceps Militaris That Induces Apoptosis, K.-C. Bai, F. Sheu
Journal of Food and Drug Analysis
Cordyceps militaris is a dietary therapeutic fungus that is an important model species in Cordyceps research. In this study, we purified a novel protein from the fruit bodies of C. militaris and designated it as Cordyceps militaris protein (CMP). CMP has a molecular mass of 18.0 kDa and is not glycosylated. Interestingly, CMP inhibited cell viability in murine primary cells and other cell lines in a time- and dose-dependent manner. Using trypan blue staining and a lactate dehydrogenase release assay, we showed that CMP caused cell death in the murine hepatoma cell line BNL 1MEA.7R.1. Furthermore, the frequency of BNL …
Corrigendum To “Investigation Of Borneols Sold In Taiwan By Chiral Gas Chromatography” (Journal Of Food And Drug Analysis (2018) 26(1) (348–352), (S1021949816301612) (10.1016/J.Jfda.2016.10.012)),
2020
Taiwan Food and Drug Administration
Corrigendum To “Investigation Of Borneols Sold In Taiwan By Chiral Gas Chromatography” (Journal Of Food And Drug Analysis (2018) 26(1) (348–352), (S1021949816301612) (10.1016/J.Jfda.2016.10.012)), T.-J. Ho, C.-C. Hung, T.-L. Shih, L.-M. Yiin, H.-P. Chen
Journal of Food and Drug Analysis
Full Text: 1. In Graphic abstract, Figure 4 and Table 2, the labels for (+)-isoborneol and (−)-isoborneol should be switched. 2. 1. In Figure 1, the labels for d-isoborneol and l-isoborneol should be switched. 3. On Page 349, in Figure 2, the label for (+)-isoborneol should be (−)-isoborneol. 4. On Page 350, in Section 3.1, the first line of the second paragraph, “(+)-Isoborneol, synthesized as previously described …. ” should be “(−)-Isoborneol, synthesized as previously described …. ”. 5. On Page 351, in the legend for Figure 4, the order of (+)-isoborneol, (−)-isoborneol should be switched. © 2018
Therapeutic Effects Of Lactobacillus Paracasei Subsp. Paracasei Ntu 101 Powder On Dextran Sulfate Sodium-Induced Colitis In Mice,
2020
Taiwan Food and Drug Administration
Therapeutic Effects Of Lactobacillus Paracasei Subsp. Paracasei Ntu 101 Powder On Dextran Sulfate Sodium-Induced Colitis In Mice, C.-L. Chen, P.-Y. Hsu, T.-M. Pan
Journal of Food and Drug Analysis
Ulcerative colitis (UC) is a form of inflammatory bowel disease (IBD) whose exact cause is still unclear. Disruption of the intestinal microflora is considered one of the main causes of the disease. Lactobacillus paracasei subsp. paracasei NTU 101 (NTU 101) is a multifunctional strain that has been shown in previous studies to possess anti-inflammatory properties and to exert a modulatory effect on intestinal bacteria associated with certain pathogenic mechanisms of IBD. In the current study, we investigated the effects of NTU 101 on dextran sulfate sodium (DSS)-induced colitis in a mouse model. Colitis was induced in male C57BL/6 mice (total …
Down-Regulation Of Partial Substitution For Staple Food By Oat Noodles On Blood Lipid Levels: A Randomized, Double-Blind, Clinical Trial,
2020
Taiwan Food and Drug Administration
Down-Regulation Of Partial Substitution For Staple Food By Oat Noodles On Blood Lipid Levels: A Randomized, Double-Blind, Clinical Trial, M.-Y. Liao, Y.-C. Shen, H.-F. Chiu, S.-M. Ten, Y.-Y. Lu, Y.-C. Han, K. Venkatakrishnan, S.-F. Yang, C.-K. Wang
Journal of Food and Drug Analysis
This clinical trial was conducted to assess the lipid-lowering activity of oat noodles by replacing partial staple food (wheat or rice noodle) in normal and mildly hypercholesterolemic subjects. Totally 84 healthy and mild hypercholesterolemic subjects were recruited and divided into 2 groups as experimental (oat noodles) and placebo (wheat noodles) and instructed to consume 100 g of oat noodles or wheat noodles (replacing one or two meals/day) for 10 weeks and followed by 2 weeks of follow up (without noodle consumption). Various anthropometric measurements and biochemical analysis were carried out during initial (baseline), 2nd, 6th, 10th and 12th week (follow-up). …
A Steroidal Derivative From Trigonella Foenum Graecum L. That Induces Apoptosis In Vitro And In Vivo,
2020
Taiwan Food and Drug Administration
A Steroidal Derivative From Trigonella Foenum Graecum L. That Induces Apoptosis In Vitro And In Vivo, R.S. Thakur, B. Ahirwar
Journal of Food and Drug Analysis
Trigonella foenum graecum L. is a dietary herb used in traditional medicine system. In this study, we investigated the cytotoxicity, antitumor, antimetastatic and antiangiogenic effect of the steroidal compound, ethyl iso-allocholate isolated from T. foenum graecum L. seeds against A549 lung cancer cells in vitro and in vivo. Among all the isolated compounds, the ethyl iso-allocholate rendered the highest cytotoxicity potential. It showed least percentage cell viability in trypan blue assay and lowest nuclei count in hoechst staining. The caspase glo assay and western blot analysis showed the significant caspase 3 cleavage, indicating caspase dependent apoptosis. Consistent with the in …
Multivariate Optimization Of Mebeverine Analysis Using Molecularly Imprinted Polymer Electrochemical Sensor Based On Silver Nanoparticles,
2020
Taiwan Food and Drug Administration
Multivariate Optimization Of Mebeverine Analysis Using Molecularly Imprinted Polymer Electrochemical Sensor Based On Silver Nanoparticles, A. Nezhadali, G.A. Bonakdar
Journal of Food and Drug Analysis
Thin film of a moleculary imprinted polymer (MIP) based on electropolymerization method with sensitive and selective binding sites for mebeverine (MEB) was developed. This film was cast on pencil graphite electrode (PGE) by electrochemical polymerization in solution of pyrrole (PY) and template MEB via cyclic voltammetry scans and further electrodeposition of silver nanoparticles (AgNPs). Several parameters controlling the performance of the silver nano particles MIP pencil graphite electrode (AgNPs-MIP-PGE) including concentration of PY(mM) concentration of mebeverine (mM), number of cycles in electropolymerization, scan rate of CV process (mV. s−1), deposition time of AgNPs on to the MIP surface (s), stirring …
Isoprenyl Phenolic Ethers From The Termite Nest-Derived Medicinal Fungus Xylaria Fimbriata,
2020
Taiwan Food and Drug Administration
Isoprenyl Phenolic Ethers From The Termite Nest-Derived Medicinal Fungus Xylaria Fimbriata, M.-C. Chen, G.-J. Wang, Y.-H. Kuo, Y.-R. Chiang, T.-Y. Cho, Y.-M. Ju, T.-H. Lee
Journal of Food and Drug Analysis
Seven new isoprenyl phenolic ethers, namely fimbriethers A‒G (1‒7), were isolated from the fermented broth of the termite nest-derived medicinal fungus Xylaria fimbriata YMJ491. Their structures were determined by spectroscopic data analysis and compared with those reported. The effects of all the isolates at a concentration of 100 μM on the inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cells were evaluated, and all of them exhibited NO production inhibitory activity with Emax values ranging from 4.6 ± 2.0% to 49.7 ± 0.5% without significant cytotoxicity. In addition, these seven compounds did not alter phenylephrine-induced …
Hantzsch Pre-Column Derivatization For Simultaneous Determination Of Alendronate Sodium And Its Pharmacopoeial Related Impurity: Comparative Study With Synchronous Fluorometry Using Fluorescamine,
2020
Taiwan Food and Drug Administration
Hantzsch Pre-Column Derivatization For Simultaneous Determination Of Alendronate Sodium And Its Pharmacopoeial Related Impurity: Comparative Study With Synchronous Fluorometry Using Fluorescamine, A.F. El-Yazbi, E.I. El-Kimary, R.M. Youssef
Journal of Food and Drug Analysis
High performance liquid chromatographic (HPLC) method with a pre-column derivatization based on Hantzsch condensation reaction was applied for simultaneous determination of alendronate sodium (ALN) and its main related impurity, 4-Aminobutanoic acid (ABA) at its pharmacopeial limit. The separation of colored condensation products of ALN and ABA were achieved on Agilent Zobrax Eclipse SB-C18 analytical column (250 × 4.6 mm, 5 μm) using a mobile phase composed of acetonitrile–0.1 M acetate buffer, pH 5.0 (15:85, v/v). The flow rate was 1 mL min−1. The detection was carried out at 340 nm using photo-diode array detector. Peak areas were used for the …
