Immunology and Infectious Disease Commons^™

Investigating The Potential Of Sodium Butyrate To Control Salmonella Enteritidis In Poultry, Anamika Gupta

Graduate Theses and Dissertations

Salmonella Enteritidis (SE) is a major poultry associated food borne pathogen that causes enteric illnesses in humans. Despite using various pre-harvest and post-harvest intervention strategies to reduce Salmonellosis, SE infection is still an extensive problem in the poultry industry with increased incidences since SE has developed multiple strategies to adapt in the chicken intestinal tract particularly in the ceca. Therefore, reducing SE in the intestine of chickens would reduce contamination of poultry derived foods and minimize the risk of human infection. Short chain fatty acids such as butyrate are microbial metabolites known to modulate inflammatory response. In this dissertation, the …

Evaluation Of Anthelmintic Therapies In A Fall Calving Beef Cowherd, Laine Zammit

Graduate Theses and Dissertations

Internal parasitism inevitability prompts economic loss in beef cattle production by decreasing growth performance and reproductive traits. Today, the most widely used class of anthelmintic used to treat parasitism, is the macrocyclic lactone. Many studies have conflicting results on the efficacy of macrocyclic lactones (ML) efficacy against internal parasitism. The objective of this study was to evaluate the effectiveness of moxidectin and eprinomectin, two of the MLs, on cow performance. Multiparous fall calving, crossbred beef cows (n = 106) were allocated randomly to 1 of 3 anthelmintic treatments: 1) Negative control (CON), in which cows did not receive an anthelmintic, …

Effect Of Zelnate Administered As A Metaphylactic Upon Initial Processing Of High-Risk, Newly Received Beef Calves On Performance And Morbidity, Brady Martin

Animal Science Undergraduate Honors Theses

Bovine Respiratory Disease (BRD) is the most prominent and costly ailment in the stocker cattle industry today, and its prevalence has not been diminished in the last thirty years. Therefore, the objective of this study was to investigate the effects of Zelnate, a DNA immunostimulant, administered upon arrival to calves (n = 261; BW 253 ± 4.0 kg), on morbidity/mortality, performance and producer costs. Crossbred male beef calves were acquired and transported to the University of Arkansas stocker unit for a 42-d backgrounding period. Calves were allocated into treatment groups: 1) Zelnate, DNA immunostimulant administered or 2) Control, in which …

Use Of Diatomaceous Earth And Copper Oxide Wire Particles To Control Gastrointestinal Nematodes In Lambs, Olivia Jones

Animal Science Undergraduate Honors Theses

Abstract

Anthelmintic resistance (AR) urges alternatives to control gastrointestinal nematodes (GIN). Copper oxide wire particles (COWP) are more efficacious when used with other dewormers and little is known on efficacy of diatomaceous earth (DE) to control gastrointestinal parasites. The objective was to examine the effects of DE and COWP on GIN control. Katahdin lambs (n = 32; ~150 d of age; 25.0 ± 1.8 kg) were randomly assigned to receive: 1) DE fed at an estimated 2% dry matter intake (with the assumption of moderate consumption of bermudagrass forage and provided supplement), 2) 1g COWP, 3) both 2% DE and …

Exosomal Communication By Metastatic Osteosarcoma Cells Modulates Alveolar Macrophages To An M2 Tumor-Promoting Phenotype And Inhibits Tumoricidal Functions, Kerri Wolf

Dissertations & Theses (Open Access)

Osteosarcoma metastasizes to the lung, and there is a link between the predominance of tumor promoting immunosuppressive M2 macrophages in the metastases and poor patient survival. By contrast, M1macrophage predominance correlates with longer survival. M2 macrophages can be induced by various stimuli in the tumor microenvironment, including exosomes, which are 40- to 150-nm vesicles that are involved in intercellular communication and contribute to tumor progression and immune evasion. Recognizing that tumor cells can influence the tumor microenvironment to make it more permissive and because of the link between M2 dominance and curtailed patient survival, we evaluated the effect of …

Tobacco Enhances Bacterial-Induced Periodontal Bone Loss In Mice., Mina Iskander

Electronic Theses and Dissertations

Background: Tobacco smoking is the leading environmental risk factor for periodontal diseases. Delineation of the mechanisms underlying tobacco-induced or exacerbated periodontitis is hampered by the lack of an appropriate and reliable animal model. Hypothesis: We hypothesized that Porphyromonas-gingivalis-infected, cigarette smoke-exposed mice would represent reproducible models of acute (ligature model) and chronic (oral gavage model) tobacco-enhanced periodontitis that reflect multiple aspects of the disease noted in human smokers. Methods: In a chronic oral gavage disease model, Balb/c mice (6-8 weeks, 4 groups of n = 6 per group) were exposed to smoke produced by a Teague-10 smoking machine from 1R6F research …

Vestigial-Like 1 Is A Shared Targetable Cancer-Placenta Antigen Expressed By Pancreatic And Basal-Like Breast Cancers, Sherille Denae Bradley

Dissertations & Theses (Open Access)

Cytotoxic T lymphocyte (CTL)-based cancer immunotherapies have shown great promise for inducing clinical regression by targeting tumor-associated antigens (TAA). To expand the TAA landscape of pancreatic ductal adenocarcinoma (PDAC), we performed tandem mass spectrometry analysis of HLA class I-bound peptides from tumors of PDAC patients. This led to the identification of a shared HLA-A*0101 restricted peptide derived from co-transcriptional activator Vestigial-like 1 (VGLL1), a novel putative TAA demonstrating overexpression in multiple tumor types and low or absent transcript expression in normal tissues with the exception of placenta. VGLL1-specific CTL isolated and expanded from the blood of a male PDAC patient …

Loss Of Caspase-8 Function In Combination With Smac Mimetic Treatment Sensitizes Head And Neck Squamous Carcinoma To Radiation Through Induction Of Necroptosis., Burak Uzunparmak

Dissertations & Theses (Open Access)

Caspase-8 (CASP8) is one of the most frequently mutated genes in Head and Neck Squamous Carcinomas (HNSCC), and mutations of CASP8 are associated with poor overall survival. The distribution of these mutations in HNSCC suggests that they are likely to be inactivating. Inhibition of CASP8 has been reported to sensitize cancer cells to necroptosis, a unique cell death mechanism. Here, we evaluated how CASP8 regulates necroptosis in HSNCC using cell line models and syngeneic mouse xenografts. In vitro, knockdown of CASP8 rendered HNSCCs susceptible to necroptosis induced by a second mitochondria-derived activator of caspase (SMAC) mimetic, Birinapant, when combined …

The Role Of The Bps Immunity Repressor In The Regulation Of Pathogenic Mycobacterium Chelonae Gene Expression, Emma Freeman

Honors College

Mycobacterium tuberculosis is the leading cause of death by an infectious disease (MacNeil, 2019). In 2018, 10 million people developed tuberculosis and half a million infections were resistant to antibiotics (WHO, 2019). Nearly all members of the M. tuberculosis complex are lysogens, meaning they carry prophage, or integrated viral genomes within the host genome (Fan et al., 2016). The non-pathogenic vaccine strain (M. bovis Bacillus Calmette–Guérin (BCG)) is the exception, which suggests prophage play a role in virulence (Fan et al., 2016). Because not all prophage encode obvious virulence genes, we hypothesize that prophage impact bacterial virulence by altering bacterial …

Characterization Of Ncf1 Mutants In A Zebrafish Model Of Innate Immune Function With Human Influenza A Virus Infection, Lily Charpentier

Honors College

Seasonal influenza A virus (IAV) infections and their associated respiratory diseases are the cause of an estimated 650,000 deaths each year, according to the World Health Organization. The zebrafish (Danio rerio) is a powerful vertebrate model to study innate immune function and host-pathogen interactions as the function of neutrophils and other phagocytes can be characterized in vivo. Preliminary studies have shown an increase in neutrophil respiratory burst activity to eliminate the invading pathogen, yet little is known of all of the mechanisms involved in neutrophil function. The NADPH oxidase complex, of which neutrophil cytosolic factor 1 (Ncf1) is a key …

In Vivo Imaging Of The Respiratory Burst Response To Influenza A Virus Infection, James Thomas Seuch

Honors College

The CDC estimated that seasonal influenza A virus (IAV) infections resulted in 490,600 hospitalizations and 34,200 deaths in the US in the 2018-2019 season. The longterm goal of our research is to understand how to improve innate immune responses to IAV. During IAV infection, neutrophils and macrophages initiate a respiratory burst response where reactive oxygen species (ROS) are generated to destroy the pathogen and recruit additional immune cells. While ROS molecules, such as hydrogen peroxide, help clear the virus, the signaling cascade can also lead to excess neutrophil recruitment, hyperinflammation, and tissue damage. Regulatory mechanisms that trigger overactivation of neutrophils …

Oncolytic Viruses: Cancer Treatment Going Viral, Rhianna N. Bronson

Chancellor’s Honors Program Projects

No abstract provided.

Characterization Of A Mycoplasma Pneumoniae Cards Toxin Mutant, Nikaash Pasnoori

Honors Scholar Theses

Mycoplasma pneumoniae is a high-burden pathogen which causes mild to significant infections of the respiratory system. According to the CDC, an estimated two million cases occur yearly in the United States alone, demonstrating the widespread effect of the pathogen. In addition to being the cause of respiratory infections, M. pneumoniae has also been implicated in exacerbating pre-existing asthma conditions. These morbidities make finding a vaccine candidate a vital part of easing the healthcare burden caused by the pathogen. The current mechanism of infection is unknown, but recent evidence points to the Community Acquired Respiratory Distress Syndrome (CARDS) toxin as being …

Development Of In Vitro Models To Study The Rapid Extraintestinal Dissemination Of Salmonella., Adarsh Gopinath

Electronic Theses and Dissertations

Salmonella appears in the bloodstream of mice in as little as 15 minutes after oral inoculation and establishes persistent colonies in the spleen and liver. While its pathway to blood is undetermined, this phenomenon is dependent on the activity of Salmonella pathogenicity island 2 (SPI-2) coded type III secretion system (T3SS) and CD18+ phagocytes. We hypothesize that dendritic cells associated with the basal face of the gut epithelium, that are naturally migratory and known to sample for luminal antigens directly transport Salmonella to the bloodstream. This process comprises of at least two phases, dissociation and reverse transmigration. We define dissociation …

Heterogeneous Nuclear Ribonucleoprotein K (Hnrnp K) Overexpression And Its Interaction With Runx1 Rna In Acute Myeloid Leukemia, Marisa Aitken

Dissertations & Theses (Open Access)

Acute myeloid leukemia (AML) is an often devastating hematologic malignancy with 5-year overall survival lingering near 20%. Acquiring a deeper understanding of molecular underpinnings of leukemogenesis will provide a basis for developing more effective therapeutic strategies for patients with AML.

Here, we identified overexpression of hnRNP K as a recurrent abnormality in a subset (~20%) of AML patients. High levels of this RNA-binding protein associated with inferior clinical outcomes in de novo AML. Thus, to evaluate its putative oncogenic capacity in myeloid disease, we overexpressed hnRNP K in murine hematopoietic stem and progenitor cells isolated from fetal liver cells (FLCs). …

A Review Of Human Immunodeficiency Virus, Sophie Silver

Senior Honors Theses

Human immunodeficiency virus, also referred to as HIV, is a devastating virus which has infected millions. Characterized as a retrovirus, HIV has an RNA genome, which is reverse transcribed into DNA upon entry into the host cell. HIV primarily affects CD4⁺ T cells and is diagnosed by the significant reduction of CD4⁺ T cells. While no cure has been discovered yet, antiretroviral therapy (ART) has been demonstrated as an effective treatment option. In the progression of HIV, additional HIV-associated diseases may arise, including HIV-associated psoriasis and sensory neuropathy. In addition to the use of ART, clinicians often prescribe …

Tumor Necrosis Factor-Alpha Signaling May Contribute To Chronic West Nile Virus Post-Infectious Proinflammatory State, A. Arturo Leis, Marie F. Grill, Brent P. Goodman, Syed B. Sadiq, David J. Sinclair, Parminder J.S. Vig, Fengwei Bai

Faculty Publications

Background: West Nile virus (WNV) causes a spectrum of human disease ranging from a febrile illness (WNV fever) to severe neuroinvasive disease (meningitis, encephalitis, acute flaccid paralysis). Since WNV gained entry into North America in 1999, clinicians caring for WNV survivors have observed persistent neurological symptoms occurring long-after the production of neutralizing antibodies and clearance of the virus. Accordingly, alternative pathogeneses other than direct viral invasion have been hypothesized to explain these post-infectious symptoms. The dominant hypothesis is that antiviral inflammatory responses triggered initially to clear WNV may persist to promote a post-infectious proinflammatory state.

Methods: In 4 serologically-confirmed …

Grain Marketing 2020: Survival And Covid-19, Robert Tigner, Jessica J. Groskopf

Extension Farm and Ranch Management News

First paragraph:

Let’s state the obvious: this isn’t a typical year and we all know it. For farmers, it is a survival year. Why? And what might be done to make it through to the other side of the COVID-19 pandemic? This article will discuss how to build a marketing strategy to assure that the farm continues to the next crop year.

Conclusion

This year is shaping up to be a survival year for crop producers. Crop producers need to know at what crop price they will recapture all of the cash they will spend this year. Implementing a marketing …

Has Covid-19 Impacted Price-Weight Relationships And Value Of Gain?, Elliott James Dennis

Extension Farm and Ranch Management News

First paragraph:

In the beginning of COVID-19, much of the attention was focused on managing the redirection of meat product from food service to retail stores. This past month has keenly focused on packing plant closures due to COVID-19 cases among workers and how to manage the supply of fat cattle already ready for slaughter. Fat cattle available for slaughter either cannot get bids or bids are significantly below breakeven prices leaving feedlots with decisions to be made about marketing and placements. The April 2020 Cattle on Feed report (https://usda.library.cornell.edu/concern/publications/m326m174z) revealed March placement decisions. Placements were down about …

Identifying Novel Triggers Of The Intracellular Pathogen Response (Ipr) In C. Elegans, Catherine T. Byrnes, Jessica N. Sowa

Biology Student Work

The intracellular pathogen response (IPR) is a cell signaling pathway found in C. elegans that is triggered when pathogenic microorganisms invade, and immune responses attempt to eliminate the threat. Due to the relative simplicity of C. elegans, they are an excellent model organism to analyze the cell signaling pathways triggered by various pathogens. They lack the complex immune systems of larger organisms, making it easier to study the involved cell signaling pathways. Past studies have shown that the IPR can also be triggered via intestinal wounding. Heat stress, viral infection, and proteasome stress are all known triggers of the IPR. …