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Checklist Of Bloodfeeding Mites (Acari: Spinturnicidae) From The Wings Of Bats (Mammalia: Chiroptera) In The Manú Biosphere Reserve, Peru, Donald D. Gettinger 2018 Manter Laboratory of Parasitology

Checklist Of Bloodfeeding Mites (Acari: Spinturnicidae) From The Wings Of Bats (Mammalia: Chiroptera) In The Manú Biosphere Reserve, Peru, Donald D. Gettinger

MANTER: Journal of Parasite Biodiversity

A survey collection of mites of the family Spinturnicidae from Peruvian bats includes 11 species of Periglischrus (acutisternus, gameroi, grandisoma, herrerai, hopkinsi, iheringi, micronycteridis, ojasti, paracutisternus, paravargasi, and ramirezi) and 2 Spinturnix (americanus and bakeri); almost all represent new locality records. This survey collection is available for further study at the following repositories: The Harold W. Manter Laboratory of Parasitology, University of Nebraska–Lincoln; the Field Museum of Natural History, Chicago; and the Laboratório de Espeleobiologia y Acarologia, Universidad Nacional Autónoma de México. When spinturnicid mites are collected to avoid cross-contamination by mites among species of bats, parasitic associations are ...


Acarine Biodiversity In Ecuador: Two New Species Of Endoparasitic Chiggers (Acarina: Trombiculidae) From Terrestrial Andean Anurans, Ricardo Guerrero, Mario Humberto Yánez-Muñoz 2018 Universidad Central de Venezuela

Acarine Biodiversity In Ecuador: Two New Species Of Endoparasitic Chiggers (Acarina: Trombiculidae) From Terrestrial Andean Anurans, Ricardo Guerrero, Mario Humberto Yánez-Muñoz

MANTER: Journal of Parasite Biodiversity

Two species of endoparasitic chiggers, Vercammenia neotropica n. sp. and Microtrombicula ecuadorensis n. sp., are described, both found in terrestrial anurans of the genus Pristimantis collected in the Andes of Ecuador. A new combination is proposed for Trombicula yorkei Sambon, 1928 as Microtrombicula yorkei (Sambon, 1928) n. comb., and Schoengastia lynni Ewing, 1942 is placed as incertae sedis. This is the first record of species of the genus Vercammenia in the Neotropical region.

Dos especies de ácaros endoparásitos, Vercammenia neotropica n. sp. y Microtrombicula ecuadorensis n. sp., son descritas, ambas encontradas en anuros terrestres del género Pristimantis recolectados en ...


Drug-Resistance And Population Structure Of Plasmodium Falciparum Across The Democratic Republic Of Congo Using High-Throughput Molecular Inversion Probes, Ozkan Aydemir, Nicholas J. Hathaway, Patrick W. Marsh, Alice Tran, Thomas Reimonn, Jeffrey A. Bailey 2018 University of Massachusetts Medical School

Drug-Resistance And Population Structure Of Plasmodium Falciparum Across The Democratic Republic Of Congo Using High-Throughput Molecular Inversion Probes, Ozkan Aydemir, Nicholas J. Hathaway, Patrick W. Marsh, Alice Tran, Thomas Reimonn, Jeffrey A. Bailey

Open Access Articles

A better understanding of the drivers of the spread of malaria parasites and drug resistance across space and time is needed. These drivers can be elucidated using genetic tools. Here, a novel molecular inversion probe (MIP) panel targeting all major drug-resistance mutations and a set of microsatellites was used to genotype Plasmodium falciparum infections of 552 children from the 2013-2014 Demographic and Health Survey conducted in the Democratic Republic of the Congo (DRC). Microsatellite-based analysis of population structure suggests that parasites within the DRC form a homogeneous population. In contrast, sulfadoxine-resistance markers in dihydropteroate synthase show marked spatial structure with ...


Bacillus Thuringiensis Cry5b Protein As A New Pan-Hookworm Cure, Yan Hu, Thanh-thanh Nguyen, Alice C. Lee Y, Joseph F. Urban Jr, Melanie M. Miller, Bin Zhan, David Koch, Jason B. Noon, Ricardo Toshio. Fujiwara, Dwight D. Bowman, Gary R. Ostroff, Raffi V. Aroian 2018 University of Massachusetts Medical School

Bacillus Thuringiensis Cry5b Protein As A New Pan-Hookworm Cure, Yan Hu, Thanh-Thanh Nguyen, Alice C. Lee Y, Joseph F. Urban Jr, Melanie M. Miller, Bin Zhan, David Koch, Jason B. Noon, Ricardo Toshio. Fujiwara, Dwight D. Bowman, Gary R. Ostroff, Raffi V. Aroian

Open Access Articles

Hookworms are intestinal nematode parasites that infect nearly half a billion people and are globally one of the most important contributors to iron-deficiency anemia. These parasites have significant impacts in developing children, pregnant women and working adults. Of all the soil-transmitted helminths or nematodes (STNs), hookworms are by far the most important, with disease burdens conservatively estimated at four million DALYs (Disability-Adjusted Life Years) and with productivity losses of up to US$139 billion annually. To date, mainly one drug, albendazole is used for hookworm therapy in mass drug administration, which has on average approximately 80% cure rate that is ...


Disruption Of Tblpn Expression In T. Brucei By Rna Interference, Matthew Serbonich 2018 The College at Brockport

Disruption Of Tblpn Expression In T. Brucei By Rna Interference, Matthew Serbonich

Senior Honors Theses

Trypanosoma brucei, the causative agent of human African trypanosomiasis, also known as African sleeping sickness, expresses a protein known as TbLpn which plays a critical role in arginine methylation and phospholipid synthesis within T. brucei. In order to determine the effect and potential medical benefits of downregulated TbLpn expression in T. brucei, RNA interference was used by transfecting T. brucei with plasmid DNA through electroporation. After conducting this experimental protocol, T. brucei was unable to be grown successfully in media following transfection and the effect of downregulated TbLpn in T. brucei requires further investigation in order to determine its potential ...


The Small Rna Complement Of Adult Schistosoma Haematobium., Andreas J Stroehlein, Neil D Young, Pasi K Korhonen, Ross S Hall, Aaron R Jex, Bonnie L Webster, David Rollinson, Paul J Brindley, Robin B Gasser 2018 George Washington University

The Small Rna Complement Of Adult Schistosoma Haematobium., Andreas J Stroehlein, Neil D Young, Pasi K Korhonen, Ross S Hall, Aaron R Jex, Bonnie L Webster, David Rollinson, Paul J Brindley, Robin B Gasser

Microbiology, Immunology, and Tropical Medicine Faculty Publications

BACKGROUND: Blood flukes of the genus Schistosoma cause schistosomiasis-a neglected tropical disease (NTD) that affects more than 200 million people worldwide. Studies of schistosome genomes have improved our understanding of the molecular biology of flatworms, but most of them have focused largely on protein-coding genes. Small non-coding RNAs (sncRNAs) have been explored in selected schistosome species and are suggested to play essential roles in the post-transcriptional regulation of genes, and in modulating flatworm-host interactions. However, genome-wide small RNA data are currently lacking for key schistosomes including Schistosoma haematobium-the causative agent of urogenital schistosomiasis of humans.

METHODOLOGY: MicroRNAs (miRNAs) and other ...


Permission To Publish Agreement, Concepts In Animal Parasitology, University of Nebraska-Lincoln 2018 University of Nebraska - Lincoln

Permission To Publish Agreement, Concepts In Animal Parasitology, University Of Nebraska-Lincoln

Concepts in Animal Parasitology Textbook

Blank Permission to Publish agreement form for authors of the Concepts in Animal Parasitology textbook/open educational resource.


Investigation Of Bradyzoite Differentiation Initiation In Toxoplasma Gondii, Harim I. Won, Paul H. Davis Ph.D. 2018 University of Nebraska at Omaha

Investigation Of Bradyzoite Differentiation Initiation In Toxoplasma Gondii, Harim I. Won, Paul H. Davis Ph.D.

Theses/Capstones/Creative Projects

Like other eukaryotic organisms, Toxoplasma gondii promoters feature both constitutive and life-stage regulated cis-elements. Using a transcriptomic microarray approach, a cluster of transcripts upregulated early during bradyzoite differentiation was identified. Computational analysis of the promoter regions of these “up-early” transcripts identified a shared upstream consensus motif, a putative transcription factor binding site. Using a dual luciferase assay adapted for recombinational cloning and reporter gene quantification by qPCR, we demonstrate developmental stage-specific expression of the luciferase reporter gene inserted downstream of the transcription factor binding site. The shared consensus motif was found to be an autonomous cis-element by conversion ...


Analysis Of Antimicrobial Peptide Efficacy Against Chytridiomycosis From Skin Secretions Of Columbia Spotted Frogs (Lithobates Luteiventris), Emma Esposito 2018 Carroll College

Analysis Of Antimicrobial Peptide Efficacy Against Chytridiomycosis From Skin Secretions Of Columbia Spotted Frogs (Lithobates Luteiventris), Emma Esposito

Carroll College Student Undergraduate Research Festival

Amphibian populations have been declining in size in recent years. A major contributing factor to this decline is the fungal disease chytridiomycosis. Chytridiomycosis occurs when the zoospores of the fungus Batrachochytrium dendrobatidis (Bd) imbed into amphibian skin and disrupt the homeostatic functions the skin provides, leading to death in most amphibians. Amphibian skin can produce antimicrobial peptides (AMPs) that inhibit Bd infection. The goal of this experiment was to determine whether or not the amount and type of AMPs a frog produces affect its ability to defend against chytridiomycosis. Columbia spotted frogs were chosen as a model organism because they ...


Identification Of Novel Hits Against, Ala A. Azhari 2018 University of South Florida

Identification Of Novel Hits Against, Ala A. Azhari

Graduate Theses and Dissertations

Leishmaniasis is a disease caused by obligate intracellular parasites of the genus Leishmania, including 20 species that are pathogenic to humans. Female sand fly is the known vector that can transmit the disease. Visceral leishmaniasis is the severe form of the disease that affects internal organs and can be fatal with inappropriate diagnosis or treatment. Leishmania donovani is the causative agent of visceral leishmaniasis. Approximately 350 million in 89 countries are at risk of infection. Around 2 million new cases are reported annually with 500,000 of these are visceral leishmaniasis. Current drug therapies are inadequate due to their toxicity ...


Recovering Parasites From Mummies And Coprolites: An Epidemiological Approach, Morgana Camacho, Adauto Araújo, Johnica J. Morrow, Jane E. Buikstra, Karl Reinhard 2018 Escola Nacional de Saúde Pública Sergio Arouca/Fundação Oswaldo Cruz

Recovering Parasites From Mummies And Coprolites: An Epidemiological Approach, Morgana Camacho, Adauto Araújo, Johnica J. Morrow, Jane E. Buikstra, Karl Reinhard

Karl Reinhard Papers/Publications

In the field of archaeological parasitology, researchers have long documented the distribution of parasites in archaeological time and space through the analysis of coprolites and human remains. This area of research defined the origin and migration of parasites through presence/absence studies. By the end of the 20th century, the field of pathoecology had emerged as researchers developed an interest in the ancient ecology of parasite transmission. Supporting studies were conducted to establish the relationships between parasites and humans, including cultural, subsistence, and ecological reconstructions. Parasite prevalence data were collected to infer the impact of parasitism on human health. In ...


Creative Commons Attribution-Noncommercial 4.0 International Public License (Cc By-Nc 4.0), Creative Commons 2018 University of Nebraska - Lincoln

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Concepts in Animal Parasitology Textbook

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Information For Authors [Slides]: Concepts In Animal Parasitology, Sue Ann Gardner, Scott Lyell Gardner 2018 University of Nebraska - Lincoln

Information For Authors [Slides]: Concepts In Animal Parasitology, Sue Ann Gardner, Scott Lyell Gardner

Concepts in Animal Parasitology Textbook

Broadcast via Zoom live online, the following are the slides from an information session held for authors and reviewers of the Concepts in Animal Parasitology (working title: Animal Parasitology Concepts) textbook. The work will be written collaboratively and published in 2019 as an open educational resource under a CC BY-NC 4.0 International license.


Instructions For Authors, Concepts In Animal Parasitology, Sue Ann Gardner, Scott Lyell Gardner 2018 University of Nebraska - Lincoln

Instructions For Authors, Concepts In Animal Parasitology, Sue Ann Gardner, Scott Lyell Gardner

Concepts in Animal Parasitology Textbook

Instructions for authors for the Concepts in Animal Parasitology free, online textbook.


Manuscript Preparation Guidelines, Concepts In Animal Parasitology, Paul Royster, Sue Ann Gardner, Scott Lyell Gardner 2018 University of Nebraska - Lincoln

Manuscript Preparation Guidelines, Concepts In Animal Parasitology, Paul Royster, Sue Ann Gardner, Scott Lyell Gardner

Concepts in Animal Parasitology Textbook

Zea Books manuscript preparation guidelines for authors of the Concept in Animal Parasitology free, online textbook.


Template For Authors For Part Two (Endoparasites) And Part Three (Ectoparasites And Parasitoids), Concepts In Animal Parasitology, Sue Ann Gardner, Scott Lyell Gardner 2018 University of Nebraska - Lincoln

Template For Authors For Part Two (Endoparasites) And Part Three (Ectoparasites And Parasitoids), Concepts In Animal Parasitology, Sue Ann Gardner, Scott Lyell Gardner

Concepts in Animal Parasitology Textbook

Support material for authors of the open educational resource, Concepts in Animal Parasitology, Part 2 (Endoparasites) and Part 3 (Ectoparasites and Parasitoids).


References Style Guide, Concepts In Animal Parasitology, Sue Ann Gardner, Scott Lyell Gardner 2018 University of Nebraska - Lincoln

References Style Guide, Concepts In Animal Parasitology, Sue Ann Gardner, Scott Lyell Gardner

Concepts in Animal Parasitology Textbook

References style guide for authors of the Concepts in Animal Parasitology free, online textbook.


Analysis Of Antimicrobial Peptide Efficacy Against Chytridiomycosis From Skin Secretions Of Columbia Spotted Frogs (Lithobates Luteiventris), Emma Esposito 2018 Carroll College, Helena, MT

Analysis Of Antimicrobial Peptide Efficacy Against Chytridiomycosis From Skin Secretions Of Columbia Spotted Frogs (Lithobates Luteiventris), Emma Esposito

Life and Environmental Sciences Undergraduate Theses

Amphibian populations have been declining in size in recent years. A major contributing factor to this decline is the fungal disease chytridiomycosis. Chytridiomycosis occurs when the zoospores of the fungus Batrachochytrium dendrobatidis (Bd) imbed into amphibian skin and disrupt the homeostatic functions the skin provides, leading to death in most amphibians. Amphibian skin can produce antimicrobial peptides (AMPs) that inhibit Bd infection. The goal of this experiment was to determine whether or not the amount and type of AMPs a frog produces affect its ability to defend against chytridiomycosis. Columbia spotted frogs were chosen as a model organism because they ...


Sequence Analysis Of The Potato Aphid Macrosiphum Euphorbiae Transcriptome Identified Two New Viruses, Marcella A. Texeira, Noa Sela, Hagop S. Atamian, Ergude Bao, Rita Chaudhury, Jacob MacWilliams, Jiangman He, Sophie Mantelin, Thomas Girke, Isgouhi Kaloshian 2018 University of California, Riverside

Sequence Analysis Of The Potato Aphid Macrosiphum Euphorbiae Transcriptome Identified Two New Viruses, Marcella A. Texeira, Noa Sela, Hagop S. Atamian, Ergude Bao, Rita Chaudhury, Jacob Macwilliams, Jiangman He, Sophie Mantelin, Thomas Girke, Isgouhi Kaloshian

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The potato aphid, Macrosiphum euphorbiae, is an important agricultural pest that causes economic losses to potato and tomato production. To establish the transcriptome for this aphid, RNA-Seq libraries constructed from aphids maintained on tomato plants were used in Illumina sequencing generating 52.6 million 75±105 bp paired-end reads. The reads were assembled using Velvet/Oases software with SEED preprocessing resulting in 22,137 contigs with an N50 value of 2,003bp. After removal of contigs from tomato host origin, 20,254 contigs were annotated using BLASTx searches against the non-redundant protein database from the National Center for Biotechnology Information ...


Down-Regulation Of Microrna-203-3p Initiates Type 2 Pathology During Schistosome Infection Via Elevation Of Interleukin-33, Xing He, Jun Xie, Yange Wang, Xiaobin Fan, Qin Su, Yue Sun, Nanhang Lei, Dongmei Zhang, Guangping Gao, Weiqing Pan 2018 Second Military Medical University

Down-Regulation Of Microrna-203-3p Initiates Type 2 Pathology During Schistosome Infection Via Elevation Of Interleukin-33, Xing He, Jun Xie, Yange Wang, Xiaobin Fan, Qin Su, Yue Sun, Nanhang Lei, Dongmei Zhang, Guangping Gao, Weiqing Pan

Open Access Articles

The type 2 immune response is the central mechanism of disease progression in schistosomiasis, but the signals that induce it after infection remain elusive. Aberrant microRNA (miRNA) expression is a hallmark of human diseases including schistosomiasis, and targeting the deregulated miRNA can mitigate disease outcomes. Here, we demonstrate that efficient and sustained elevation of miR-203-3p in liver tissues, using the highly hepatotropic recombinant adeno-associated virus serotype 8 (rAAV8), protects mice against lethal schistosome infection by alleviating hepatic fibrosis. We show that miR-203-3p targets interleukin-33 (IL-33), an inducer of type 2 immunity, in hepatic stellate cells to regulate the expansion and ...


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