Open Access. Powered by Scholars. Published by Universities.®

Immunopathology Commons

Open Access. Powered by Scholars. Published by Universities.®

263 Full-Text Articles 814 Authors 26017 Downloads 27 Institutions

All Articles in Immunopathology

Faceted Search

263 full-text articles. Page 1 of 11.

The Rodent Parvovirus H-1 And Its Potential In Combination With Tien Hsien Liquid As A Treatment For Breast Cancer, Hayley I. Spires 2016 Southeastern University - Lakeland

The Rodent Parvovirus H-1 And Its Potential In Combination With Tien Hsien Liquid As A Treatment For Breast Cancer, Hayley I. Spires

Selected Honors Theses

Breast cancer is responsible for 12% of cancer diagnoses each year, and it is the leading cause of cancer-related mortality in women worldwide. Current treatments have provided some success in combatting the disease but are not considered a final solution. The framework for how researchers approach cancer has continued to change, and this includes the introduction of oncolytic viruses as novel therapeutics for cancer. The rodent parvovirus H-1 has shown strong potential in clinical and subclinical trials, but its S phase dependency limits its usefulness against cancer stem cell populations. Tien Hsien Liquid is commerically available, nontoxic, and has shown ...


Distinct Differences On Neointima Formation In Immunodeficient And Humanized Mice After Carotid Or Femoral Arterial Injury, Jill Moser, Joris van Ark, Marcory C. van Dijk, Dale L. Greiner, Leonard D. Shultz, Harry van Goor, Jan-Luuk Hillebrands 2016 University of Groningen

Distinct Differences On Neointima Formation In Immunodeficient And Humanized Mice After Carotid Or Femoral Arterial Injury, Jill Moser, Joris Van Ark, Marcory C. Van Dijk, Dale L. Greiner, Leonard D. Shultz, Harry Van Goor, Jan-Luuk Hillebrands

Open Access Articles

Percutaneous coronary intervention is widely adopted to treat patients with coronary artery disease. However, restenosis remains an unsolved clinical problem after vascular interventions. The role of the systemic and local immune response in the development of restenosis is not fully understood. Hence, the aim of the current study was to investigate the role of the human immune system on subsequent neointima formation elicited by vascular injury in a humanized mouse model. Immunodeficient NOD.Cg-PrkdcscidIL2rgtm1Wjl(NSG) mice were reconstituted with human (h)PBMCs immediately after both carotid wire and femoral cuff injury were induced in order to identify how differences in ...


A Chimeric Protein-Based Malaria Vaccine Candidate Induces Robust T Cell Responses Against Plasmodium Vivax Msp119, Jairo Andres Fonseca, Monica Cabrera-Mora, Balwan Singh, Joseli Oliveira-Ferreira, Josue Da Costa Lima-Junior, J. Mauricio Calvo-Calle, Jose Manuel Lozano, Alberto Moreno 2016 Emory University

A Chimeric Protein-Based Malaria Vaccine Candidate Induces Robust T Cell Responses Against Plasmodium Vivax Msp119, Jairo Andres Fonseca, Monica Cabrera-Mora, Balwan Singh, Joseli Oliveira-Ferreira, Josue Da Costa Lima-Junior, J. Mauricio Calvo-Calle, Jose Manuel Lozano, Alberto Moreno

Open Access Articles

The most widespread Plasmodium species, Plasmodium vivax, poses a significant public health threat. An effective vaccine is needed to reduce global malaria burden. Of the erythrocytic stage vaccine candidates, the 19 kDa fragment of the P. vivax Merozoite Surface Protein 1 (PvMSP119) is one of the most promising. Our group has previously defined several promiscuous T helper epitopes within the PvMSP1 protein, with features that allow them to bind multiple MHC class II alleles. We describe here a P. vivax recombinant modular chimera based on MSP1 (PvRMC-MSP1) that includes defined T cell epitopes genetically fused to PvMSP119. This vaccine candidate ...


Distinct Surveillance Pathway For Immunopathology During Acute Infection Via Autophagy And Sr-Bi, Susanne Pfeiler, Eicke Latz, Bernd Engelmann 2016 Ludwig-Maximilians-Universitat

Distinct Surveillance Pathway For Immunopathology During Acute Infection Via Autophagy And Sr-Bi, Susanne Pfeiler, Eicke Latz, Bernd Engelmann

Open Access Articles

The mechanisms protecting from immunopathology during acute bacterial infections are incompletely known. We found that in response to apoptotic immune cells and live or dead Listeria monocytogenes scavenger receptor BI (SR-BI), an anti-atherogenic lipid exchange mediator, activated internalization mechanisms with characteristics of macropinocytosis and, assisted by Golgi fragmentation, initiated autophagic responses. This was supported by scavenger receptor-induced local increases in membrane cholesterol concentrations which generated lipid domains particularly in cell extensions and the Golgi. SR-BI was a key driver of beclin-1-dependent autophagy during acute bacterial infection of the liver and spleen. Autophagy regulated tissue infiltration of neutrophils, suppressed accumulation of ...


Surveillance Immunity: An Emerging Paradigm Of Innate Defense Activation In Caenorhabditis Elegans, Read Pukkila-Worley 2016 University of Massachusetts Medical School

Surveillance Immunity: An Emerging Paradigm Of Innate Defense Activation In Caenorhabditis Elegans, Read Pukkila-Worley

Open Access Articles

No abstract provided.


Pd-L1 Expression In Ebv-Negative Diffuse Large B-Cell Lymphoma: Clinicopathologic Features And Prognostic Implications, Wei Xing, Karen A. Dresser, Rui Zhang, Andrew M. Evens, Hongbo Yu, Bruce A. Woda, Benjamin J. Chen 2016 University of Massachusetts Medical School

Pd-L1 Expression In Ebv-Negative Diffuse Large B-Cell Lymphoma: Clinicopathologic Features And Prognostic Implications, Wei Xing, Karen A. Dresser, Rui Zhang, Andrew M. Evens, Hongbo Yu, Bruce A. Woda, Benjamin J. Chen

Open Access Articles

Programmed cell death ligand 1 (PD-L1) is a cell surface glycoprotein that regulates the cellular immune response and serves as a targetable immune checkpoint molecule. PD-L1 is expressed on tumor cells and the immune microenvironment of several human malignancies, including a subset of aggressive lymphomas. We sought to investigate further the clinical and pathologic features of EBV-negative diffuse large B-cell lymphoma (DLBCL) cases that express PD-L1. Immunohistochemical staining using an anti-PD-L1 monoclonal antibody was performed on DLBCL cases from 86 patients. These patients received standard chemotherapy treatment and were followed for up to 175 months. Overall, 14 cases (16%) were ...


A Fluorescent Reporter Mouse For Inflammasome Assembly Demonstrates An Important Role For Cell-Bound And Free Asc Specks During In Vivo Infection, Te-Chen Tzeng, Stefan A. Schattgen, Brian G. Monks, Donghai Wang, Anna M. Cerny, Eicke Latz, Katherine A. Fitzgerald, Douglas T. Golenbock 2016 University of Massachusetts Medical School

A Fluorescent Reporter Mouse For Inflammasome Assembly Demonstrates An Important Role For Cell-Bound And Free Asc Specks During In Vivo Infection, Te-Chen Tzeng, Stefan A. Schattgen, Brian G. Monks, Donghai Wang, Anna M. Cerny, Eicke Latz, Katherine A. Fitzgerald, Douglas T. Golenbock

Katherine A. Fitzgerald

Inflammasome activation is associated with numerous diseases. However, in vivo detection of the activated inflammasome complex has been limited by a dearth of tools. We have developed transgenic mice that ectopically express the fluorescent adaptor protein, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and characterized the formation of assembled inflammasome complexes ("specks") in primary cells and tissues. In addition to hematopoietic cells, we have found that a stromal population in the lung tissues formed specks during the early phase of influenza infection, whereas myeloid cells showed speck formation after 2 days. In a peritonitis and group B streptococcus ...


Genomic Insights Into The Ixodes Scapularis Tick Vector Of Lyme Disease, Monika Gulia-Nuss,, Daniel R. Caffrey, Neal S. Silverman, Adam R. Wespiser, Catherine A. Hill 2016 Purdue University

Genomic Insights Into The Ixodes Scapularis Tick Vector Of Lyme Disease, Monika Gulia-Nuss,, Daniel R. Caffrey, Neal S. Silverman, Adam R. Wespiser, Catherine A. Hill

Neal Silverman

Ticks transmit more pathogens to humans and animals than any other arthropod. We describe the 2.1 Gbp nuclear genome of the tick, Ixodes scapularis (Say), which vectors pathogens that cause Lyme disease, human granulocytic anaplasmosis, babesiosis and other diseases. The large genome reflects accumulation of repetitive DNA, new lineages of retro-transposons, and gene architecture patterns resembling ancient metazoans rather than pancrustaceans. Annotation of scaffolds representing approximately 57% of the genome, reveals 20,486 protein-coding genes and expansions of gene families associated with tick-host interactions. We report insights from genome analyses into parasitic processes unique to ticks, including host 'questing ...


A Fluorescent Reporter Mouse For Inflammasome Assembly Demonstrates An Important Role For Cell-Bound And Free Asc Specks During In Vivo Infection, Te-Chen Tzeng, Stefan A. Schattgen, Brian G. Monks, Donghai Wang, Anna M. Cerny, Eicke Latz, Katherine A. Fitzgerald, Douglas T. Golenbock 2016 University of Massachusetts Medical School

A Fluorescent Reporter Mouse For Inflammasome Assembly Demonstrates An Important Role For Cell-Bound And Free Asc Specks During In Vivo Infection, Te-Chen Tzeng, Stefan A. Schattgen, Brian G. Monks, Donghai Wang, Anna M. Cerny, Eicke Latz, Katherine A. Fitzgerald, Douglas T. Golenbock

Open Access Articles

Inflammasome activation is associated with numerous diseases. However, in vivo detection of the activated inflammasome complex has been limited by a dearth of tools. We have developed transgenic mice that ectopically express the fluorescent adaptor protein, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and characterized the formation of assembled inflammasome complexes ("specks") in primary cells and tissues. In addition to hematopoietic cells, we have found that a stromal population in the lung tissues formed specks during the early phase of influenza infection, whereas myeloid cells showed speck formation after 2 days. In a peritonitis and group B streptococcus ...


Channa Striatus Tnfr-1: Molecular Cloning, Characterization And Gene Expression, Rajesh Palanisamy 2016 SRM Institute of Science and Technology

Channa Striatus Tnfr-1: Molecular Cloning, Characterization And Gene Expression, Rajesh Palanisamy

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Border Security: The Role Of Ripk3 In Epithelium Homeostasis, Kenta Moriwaki, Sakthi Balaji, Francis Ka-Ming Chan 2016 University of Massachusetts Medical School

Border Security: The Role Of Ripk3 In Epithelium Homeostasis, Kenta Moriwaki, Sakthi Balaji, Francis Ka-Ming Chan

Open Access Articles

Receptor interacting protein kinase 3 (RIPK3) is a crucial inducer of necroptosis. Its activity is controlled by interaction with other signal adaptors through the "RIP homotypic interaction motif" (RHIM). Recent studies revealed a critical function for RIPK3 in the maintenance of epithelial tissue integrity. In mice with genetic deficiency of the apoptosis adaptors FADD or caspase 8, RIPK3 promotes necroptotic cell death of epithelial cells, leading to excessive and lethal inflammation. In contrast, when FADD and caspase 8 functions are intact, RIPK3 serves as a protector of intestinal epithelial integrity by promoting injury-induced wound repair. In the latter case, RIPK3 ...


Gene Expression Of Interleukin 8 In Liver Of Japanese Flounder Paralichthys Olivaceus Infected With Edwardsiella Tarda, Osamu Kurata, Shinpei Wada 2016 Nippon Veterinary and Life Science University

Gene Expression Of Interleukin 8 In Liver Of Japanese Flounder Paralichthys Olivaceus Infected With Edwardsiella Tarda, Osamu Kurata, Shinpei Wada

2nd International Conference of Fish & Shellfish Immunology

No abstract provided.


Il-2 And Il-6 Cooperate To Enhance The Generation Of Influenza-Specific Cd8 T Cells Responding To Live Influenza Virus In Aged Mice And Humans, Xin Zhou, Jacob W. Hopkins, Chongkai Wang, Vinayak Brahmakshatriya, Susan L. Swain, George A. Kuchel, Laura Haynes, Janet E. McElhaney 2016 University of ConnecticutSchool of Medicine

Il-2 And Il-6 Cooperate To Enhance The Generation Of Influenza-Specific Cd8 T Cells Responding To Live Influenza Virus In Aged Mice And Humans, Xin Zhou, Jacob W. Hopkins, Chongkai Wang, Vinayak Brahmakshatriya, Susan L. Swain, George A. Kuchel, Laura Haynes, Janet E. Mcelhaney

Open Access Articles

An age-related decline in cytolytic activity has been described in CD8+ T cells and we have previously shown that the poor CD8+ effector T cell responses to influenza A/H3N2 challenge result from a decline in the proportion and function of these cytolytic T lymphocytes (CTL). Here, we describe that addition of exogenous cytokines to influenza-stimulated PBMC from both aged mice and humans, enhances the generation of influenza specific CD8 CTL by increasing their proliferation and survival. Our data show that the addition of IL-2 and IL-6 to splenocytes from mice previously infected with influenza virus restores the aged CD8 ...


Reassessing The Role Of The Nlrp3 Inflammasome During Pathogenic Influenza A Virus Infection Via Temporal Inhibition, Michelle D. Tate, James D.H. Ong, Jennifer K. Dowling, Julie L. McAuley, Avril B. Robertson, Eicke Latz, Grant R. Drummond, Matthew A. Cooper, Paul J. Hertzog, Ashley Mansell 2016 Monash University

Reassessing The Role Of The Nlrp3 Inflammasome During Pathogenic Influenza A Virus Infection Via Temporal Inhibition, Michelle D. Tate, James D.H. Ong, Jennifer K. Dowling, Julie L. Mcauley, Avril B. Robertson, Eicke Latz, Grant R. Drummond, Matthew A. Cooper, Paul J. Hertzog, Ashley Mansell

Open Access Articles

The inflammasome NLRP3 is activated by pathogen associated molecular patterns (PAMPs) during infection, including RNA and proteins from influenza A virus (IAV). However, chronic activation by danger associated molecular patterns (DAMPs) can be deleterious to the host. We show that blocking NLRP3 activation can be either protective or detrimental at different stages of lethal influenza A virus (IAV). Administration of the specific NLRP3 inhibitor MCC950 to mice from one day following IAV challenge resulted in hypersusceptibility to lethality. In contrast, delaying treatment with MCC950 until the height of disease (a more likely clinical scenario) significantly protected mice from severe and ...


Scaffolded Antigens In Yeast Cell Particle Vaccines Provide Protection Against Systemic Polyoma Virus Infection, Donald J. Tipper, Eva Szomolanyi-Tsuda 2016 University of Massachusetts Medical School

Scaffolded Antigens In Yeast Cell Particle Vaccines Provide Protection Against Systemic Polyoma Virus Infection, Donald J. Tipper, Eva Szomolanyi-Tsuda

Open Access Articles

Background. U65, a self-aggregating peptide scaffold, traps fused protein antigens in yeast cells. Conversion to Yeast Cell Particle (YCP) vaccines by partial removal of surface mannoproteins exposes beta-glucan, mediating efficient uptake by antigen-presenting cells (APCs). YCP vaccines are inexpensive, capable of rapid large-scale production and have potential for both parenteral and oral use. Results. YCP processing by alkaline hydrolysis exposes up to 20% of the glucan but converts scaffolded antigen and internal yeast proteins into a common aggregate, preventing selective yeast protein removal. For U65-green fluorescent protein (GFP) or U65-Apolipoprotein A1 (ApoA1) subcutaneous vaccines, maximal IgG responses in mice required ...


Rapid Diagnostics For Infectious Disease Using Noble Metal Nanoparticles, Chun-Wan Yen, Helena de Puig, Justina Tam, José Gómez-Márquez, Irene Bosch, Lee Gehrke, Kimberly Hamad-Schifferli 2016 Massachusetts Institute of Technology

Rapid Diagnostics For Infectious Disease Using Noble Metal Nanoparticles, Chun-Wan Yen, Helena De Puig, Justina Tam, José Gómez-Márquez, Irene Bosch, Lee Gehrke, Kimberly Hamad-Schifferli

UMass Center for Clinical and Translational Science Research Retreat

Rapid point-of-care (POC) diagnostic devices are needed for field-forward screening of severe acute systemic febrile illnesses such as dengue, Ebola, chikungunya, and others. Multiplexed rapid lateral flow diagnostics have the potential to distinguish among multiple pathogens, thereby facilitating diagnosis and improving patient care. We present a platform for multiplexed pathogen detection which uses gold or silver nanoparticles conjugated to antibodies to sense the presence of biomarkers for different infectious diseases. We exploit the size-dependent optical properties of Ag NPs to construct a multiplexed paperfluidic lateral flow POC sensor. AgNPs of different sizes were conjugated to antibodies that bind to specific ...


Microfluidic Platforms To Study Host-Microbiome Interactions, Abhinav Sharma, Neil Forbes, Jungwoo Lee 2016 University of Massachusetts Amherst

Microfluidic Platforms To Study Host-Microbiome Interactions, Abhinav Sharma, Neil Forbes, Jungwoo Lee

UMass Center for Clinical and Translational Science Research Retreat

About three-quarters of human body surface is exposed to dense microbial population along the gastro-intestinal tract (GIT) that accommodates around 80% of immune cells. The GIT is one of the most critical sites for metabolic and immunologic homeostasis in the body. While large-scale genomic analysis and germ-free mice have been widely used, they are limited to capture the dynamic functional interaction between host-microbiome in a humanized setting. in-vitro recapitulation of GIT physiology can be a powerful alternative for hypothesis testing in diseases like IBD and cancer.

As a first step, we developed a simple transwell assembly to closely emulate GIT ...


Interrogating Plant Cell Culture Library For Novel Antimicrobial Agents, John Solitro, Yong Zhang, Vanessa Bartolo, Alexander Popchuk, Sergey Savinov, Lin-Jun Ma, Jennifer Normanly, Tristram Seidler, Elizabeth Vierling 2016 University of Massachusetts Amherst

Interrogating Plant Cell Culture Library For Novel Antimicrobial Agents, John Solitro, Yong Zhang, Vanessa Bartolo, Alexander Popchuk, Sergey Savinov, Lin-Jun Ma, Jennifer Normanly, Tristram Seidler, Elizabeth Vierling

UMass Center for Clinical and Translational Science Research Retreat

The Plant Cell Culture Library (PCCL) at UMass Amherst contains more than 2,200 live plant cell cultures, representing diverse plant species from around the world. The availability of this collection offers a rich resource for us to discover bioactive phytochemicals and uncover their mechanisms of action. Using data-mining surveys of bioactive plant extracts, I have organized subsets of PCCL cell lines that are likely to possess antifungal, antibacterial, antiviral, anthelmintic, anti-trypanosomal, or anticancer properties, which prove to be useful when deciding which species to screen first against a specific pathogen. Another distinct advantage of using the live plant cells ...


Hiv Vaccines: Progress, Limitations And A Crispr/Cas9 Vaccine, Omar A. Garcia Martinez 2016 Augustana College, Rock Island Illinois

Hiv Vaccines: Progress, Limitations And A Crispr/Cas9 Vaccine, Omar A. Garcia Martinez

Biology: Student Scholarship & Creative Works

ABSTRACT: The HIV-1 pandemic continues to thrive due to ineffective HIV-1 vaccines. Historically, the world’s most infectious diseases, such as polio and smallpox, have been eradicated or have come close to eradication due to the advent of effective vaccines. Highly active antiretroviral therapy is able to delay the onset of AIDS but can neither rid the body of HIV-1 proviral DNA nor prevent further transmission. A prophylactic vaccine that prevents the various mechanisms HIV-1 has to evade and attack our immune system is needed to end the HIV-1 pandemic. Recent advances in engineered nuclease systems, like the CRISPR/Cas9 ...


Hla Class I Supertype Associations With Clinical Outcome Of Secondary Dengue Virus Infections In Ethnic Thais, Sasijit Vejbaesya, Rungrot Thongpradit, Siripen Kalayanarooj, Komon Luangtrakool, Panpimon Luangtrakool, Robert V. Gibbons, Duangporn Srinak, Somporn Ngammthaworn, Kusuma Apisawes, In-Kyu Yoon, Stephen J. Thomas, Richard G. Jarman, Anon Srikiatkhachorn, Sharone Green, Dasnayanee Chandanayingyong, Sangshin Park, Jennifer Friedman, Alan L. Rothman, Henry A.F. Stephens 2016 Mahidol University

Hla Class I Supertype Associations With Clinical Outcome Of Secondary Dengue Virus Infections In Ethnic Thais, Sasijit Vejbaesya, Rungrot Thongpradit, Siripen Kalayanarooj, Komon Luangtrakool, Panpimon Luangtrakool, Robert V. Gibbons, Duangporn Srinak, Somporn Ngammthaworn, Kusuma Apisawes, In-Kyu Yoon, Stephen J. Thomas, Richard G. Jarman, Anon Srikiatkhachorn, Sharone Green, Dasnayanee Chandanayingyong, Sangshin Park, Jennifer Friedman, Alan L. Rothman, Henry A.F. Stephens

Sharone Green

BACKGROUND: Human leukocyte antigen (HLA) supertypes are groups of functionally related alleles that present structurally similar antigens to the immune system.

OBJECTIVES: To analyze HLA class I supertype associations with clinical outcome in hospitalized Thai children with acute dengue illness.

METHODS: Seven hundred sixty-two patients and population-matched controls recruited predominantly in Bangkok were HLA-A and -B typed. HLA supertype frequencies were compared and tested for significant dengue disease associations using logistic regression analyses. Multivariable models were built by conducting forward stepwise selection procedures.

RESULTS: In the final logistic regression model, the HLA-B44 supertype was protective against dengue hemorrhagic fever (DHF ...


Digital Commons powered by bepress