Modified Cantilever Arrays Improve Sensitivity And Reproducibility Of Nanomechanical Sensing In Living Cells, 2018 University College London
Modified Cantilever Arrays Improve Sensitivity And Reproducibility Of Nanomechanical Sensing In Living Cells, Samadhan B. Patil, Rajai M. Al-Jehani, Hashem Etayash, Valerian Turbe, Keren Jiang, Joe Bailey, Walid Al-Akkad, Rania Soudy, Kamaljit Kaur, Rachel A. Mckendry, Thomas Thundat, Joseph W. Ndieyira
Pharmacy Faculty Articles and Research
Mechanical signaling involved in molecular interactions lies at the heart of materials science and biological systems, but the mechanisms involved are poorly understood. Here we use nanomechanical sensors and intact human cells to provide unique insights into the signaling pathways of connectivity networks, which deliver the ability to probe cells to produce biologically relevant, quantifiable and reproducible signals. We quantify the mechanical signals from malignant cancer cells, with 10 cells per ml in 1000-fold excess of non-neoplastic human epithelial cells. Moreover, we demonstrate that a direct link between cells and molecules creates a continuous connectivity which acts like a percolating ...
Characterization Of Theranostic Peptides For Glioblastoma Multiforme, 2018 Northern Michigan University
Characterization Of Theranostic Peptides For Glioblastoma Multiforme, Aaron Mellesmoen
All NMU Master's Theses
Glioblastoma multiforme (GBM) is a type of primary CNS tumor in which viable treatment options do not exist. Standard of care including tumor resection, chemotherapy, and radiation does little to extend the 5-year survival expectancy past 5.1%. Herein, two small-peptide molecules with inherent antitumor activity, blood-brain barrier permeability, and capability for tumor-specific drug deliverance and intraoperative visualization (termed theranostic) were of focus. Confocal microscopy was employed to characterize in vitro specificity of chlorotoxin, a 4 kDa scorpion venom peptide, and rBSG, the recombinant 25 kDa non-glycosylated extracellular domain of extracellular matrix metalloproteinase inducer (EMMPRIN; Basigin ...
Efficient Intracellular Delivery Of Cell-Impermeable Cargo Molecules By Peptides Containing Tryptophan And Histidine, Amir Nasrolahi Shirazi, Saghar Mozaffari, Rinzhin Tshering Sherpa, Rakesh Tiwari, Keykavous Parang
Pharmacy Faculty Articles and Research
We have previously evaluated and reported numerous classes of linear and cyclic peptides containing hydrophobic and hydrophilic segments for intracellular delivery of multiple molecular cargos. Herein, a combination of histidine and tryptophan amino acids were designed and evaluated for their efficiency in intracellular delivery of cell-impermeable phosphopeptides and the anti-HIV drug, emtricitabine. Two new decapeptides, with linear and cyclic natures, both containing alternate tryptophan and histidine residues, were synthesized using Fmoc/tBu solid-phase chemistry. The peptides were characterized and purified by using matrix-assisted laser desorption/ionization (MALDI) spectroscopy and high-performance liquid chromatography (HPLC), respectively. These peptides did not show significant ...
Disentangling The Subalpine Marshmarigold Species Complex: The Cytogeography, Phylogeography, And Systematics Of Caltha Biflora Dc., C. Chionophila Greene, And C. Leptosepala Dc. (Ranunculaceae), 2018 University of Wisconsin-Milwaukee
Disentangling The Subalpine Marshmarigold Species Complex: The Cytogeography, Phylogeography, And Systematics Of Caltha Biflora Dc., C. Chionophila Greene, And C. Leptosepala Dc. (Ranunculaceae), Keir Wefferling
Theses and Dissertations
Subalpine marshmarigolds (Caltha biflora DC., C. chionophila DC., and C. leptosepala Greene, Ranunculaceae) are herbaceous perennials that grow in western North American mountainous and subarctic regions, from Alaska and Yukon in the north to California, Arizona, and New Mexico in the south. Variation in morphology across the range has generally led to recognition of a single species, though some have described up to nine species in the complex. In this dissertation, I describe our approaches to disentangling reticulate evolution across the geographical range of the subalpine marshmarigold complex, including chromosome counts (Chapter II), genome size estimates (Chapter III), morphology (Chapters ...
Investigation For Novel Anti-Apoptotic Factors In The Neurons Of Drosophila Melanogaster, 2018 University of Tennessee, Knoxville
Investigation For Novel Anti-Apoptotic Factors In The Neurons Of Drosophila Melanogaster, Haylie Rachel Lam
Chancellor’s Honors Program Projects
No abstract provided.
Rescue Of Neocortical Circuit Deficits With Modified Bone Marrow-Derived Mesenchymal Stem Cells, Sb623, In A Rat Model Of Photothrombotic Stroke, Alexander Urry
Yale Day of Data
The following poster characterizes the effects of a novel stem cell line on treating the neural circuit deficits resulting from stroke.
Obesity, 2018 Augustana College, Rock Island Illinois
Obesity, Noah Rhodes
Global Issues in Public Health
Obesity is a worldwide issue that can be defined as a non-communicable condition which means that it is non-infectious and non-transmissible and it is defined as having a BMI over 30 even though BMI doesn’t account for the person’s muscle mass which causes some athletes to be considered obese just because they weigh a lot for their height. Diagnosing obesity can be a long process which includes a health history being taken, a general physical exam, calculation of BMI, and measuring waist circumference. Diagnosis of obesity also involves checking for problems such as high blood pressure, variation of ...
Identifying Functional Components Of The Endoplasmic Reticulum Quality Control And Degradation Factor Edem1, 2018 University of Massachusetts Amherst
Identifying Functional Components Of The Endoplasmic Reticulum Quality Control And Degradation Factor Edem1, Lydia Lamriben
The ER Degradation-Enhancing Mannosidase-Like protein 1 (EDEM1) is a critical endoplasmic reticulum (ER) quality control factor involved in identifying and directing non-native proteins to the ER-associated protein degradation (ERAD) pathway. However, its recognition and binding properties have remained enigmatic since its discovery. Here we provide evidence for an additional redox-sensitive interaction between EDEM1 and Z/NHK that requires the presence of the single Cys on the α-1 antitrypsin ERAD clients. Moreover, this Cys-dependent interaction is necessary when the proteins are isolated under stringent detergent conditions, ones in which only strong covalent interactions can be sustained. This interaction is inherent to ...
The Functions Of Setd5 And Mir-221 In Embryonic Stem Cell Differentiation, 2017 The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences
The Functions Of Setd5 And Mir-221 In Embryonic Stem Cell Differentiation, Tsai-Yu Chen
UT GSBS Dissertations and Theses (Open Access)
Embryonic stem cells (ESCs) are a widely used model system to study cellular differentiation because of their pluripotent characteristics, and ESC differentiation is an epigenetic process. In an effort to identify a new epigenetic factor that is required for ESC differentiation, the function of SETD5 in ESCs was studied for this thesis. Results show that SETD5 is essential for retinoic acid (RA)-induced differentiation of mouse ESCs and for RA-induced expression of critical developmental genes (e.g., Hoxa1 and Hoxa2) and neuron-related genes (e.g., Nestin and Pax6). SETD5 was upregulated during ESC differentiation. Additional results demonstrated that SETD5 bound ...
Transgneic Endothelin 3 Regulates Murine Pigment Production And Coat Color, 2017 Florida International University
Transgneic Endothelin 3 Regulates Murine Pigment Production And Coat Color, Javier Pino
FIU Electronic Theses and Dissertations
Pigmentation plays a protective role against damage caused by ultraviolet (UV) irradiation. Humans with fair skin and light hair have a higher susceptibility to UV-induced DNA damage that can lead to the development of skin cancers. The melanocytes found in the skin and hair follicles depend on different signaling molecules for their proper development and pigment production. α-Melanocyte Stimulating Hormone (α-msh) binds to the Melanocortin 1 receptor (Mc1r) to regulate pigment production and the switch between eumelanin and pheomelanin. Lethal yellow mice (Ay) overexpress the agouti signaling protein, which inhibits the binding of α-msh, resulting in a yellow coat ...
Morphogenesis And Growth Driven By Selection Of Dynamical Properties, 2017 The Graduate Center, City University of New York
Morphogenesis And Growth Driven By Selection Of Dynamical Properties, Yuri Cantor
All Dissertations, Theses, and Capstone Projects
Organisms are understood to be complex adaptive systems that evolved to thrive in hostile environments. Though widely studied, the phenomena of organism development and growth, and their relationship to organism dynamics is not well understood. Indeed, the large number of components, their interconnectivity, and complex system interactions all obscure our ability to see, describe, and understand the functioning of biological organisms.
Here we take a synthetic and computational approach to the problem, abstracting the organism as a cellular automaton. Such systems are discrete digital models of real-world environments, making them more accessible and easier to study then their physical world ...
The Role Of T-Box Proteins In Vertebrate Germ Layer Formation And Patterning, 2017 The Graduate Center, City University of New York
The Role Of T-Box Proteins In Vertebrate Germ Layer Formation And Patterning, Sushma Teegala
All Dissertations, Theses, and Capstone Projects
All of the tissues in triploblastic organisms, with the exception of the germ cells, arise from the three germ layers, ectoderm, mesoderm and the endoderm. The identification of the genes that underlie the differentiation of these layers is crucial to our understanding of development. T-box family proteins are DNA-binding transcriptional regulators that play important roles during germ layer formation in the early vertebrate embryo. Well-characterized members of this family, including the transcriptional activators Brachyury and VegT, are essential for the proper formation of mesoderm and endoderm, respectively. To date, T-box proteins have not been shown to play a role in ...
Intracellular Signalling Crosstalk In The Differentiation Of F9 Cells Into Extraembryonic Endoderm, 2017 The University of Western Ontario
Intracellular Signalling Crosstalk In The Differentiation Of F9 Cells Into Extraembryonic Endoderm, Tina Nicole Cuthbert
Electronic Thesis and Dissertation Repository
Mouse F9 cells differentiate into primitive extraembryonic endoderm (PrE) with retinoic acid (RA) treatment, resulting in up-regulation of Gata6, which when translated directly activates Wnt6. Canonical Wnt signalling is required for PrE differentiation, and this, like most developmental processes, requires input from one or more additional pathways, including hedgehog (Hh). The Hh pathway is regulated by GATA6, and crosstalks positively/negatively with Wnt signalling. Ihh up-regulation in F9 cells accompanies PrE induction, but a role for GATA6 or Hh pathway activation in obligatory Wnt/ß-catenin signalling is not known. To address this, I show that RA induces Ihh and altered ...
Compartmentalized Camp Responses To Prostaglandin Ep2 Receptor Activation In Human Airway Smooth Muscle Cells, 2017 University of Nevada, Reno
Compartmentalized Camp Responses To Prostaglandin Ep2 Receptor Activation In Human Airway Smooth Muscle Cells, Shailesh R. Agarwal, Kathryn Miyashiro, Htun Latt, Rennolds S. Ostrom, Robert D. Harvey
Pharmacy Faculty Articles and Research
Background and Purpose
Previous studies indicate that prostaglandin EP2 receptors (EP2Rs) selectively couple to adenylyl cyclase type 2 (AC2) in non-lipid raft domains of airway smooth muscle (ASM) cells, where they regulate specific cAMP-dependent responses. The goal of the present study was to identify the cellular microdomains where EP2Rs stimulate cAMP production.
FRET-based cAMP biosensors were targeted to different subcellular locations of primary human ASM cells. The Epac2-camps biosensor, which expresses throughout the cell, was used to measure bulk cytoplasmic responses. Epac2-MyrPalm and Epac2-CAAX were used to measure responses associated with lipid raft and non-raft regions of the ...
Fin-Fold Development In Paddlefish And Catshark And Implications For The Evolution Of The Autopod, 2017 Kennesaw State University
Fin-Fold Development In Paddlefish And Catshark And Implications For The Evolution Of The Autopod, Frank J. Tulenko, James L. Massey, Elishka Holmquist, Gabriel Kigundu, Sarah Thomas, Susan Me Smith, Sylvie Mazan, Marcus C. Davis
Marcus C Davis
Sustainability In The Curriculum: Global Warming, 2017 Georgia State University
Sustainability In The Curriculum: Global Warming, Lori Dickey
Georgia State Undergraduate Research Conference
No abstract provided.
Human Neuroblastoma Adaptation To Cobalt Chloride-Induced Hypoxia, 2017 Seton Hall University
Human Neuroblastoma Adaptation To Cobalt Chloride-Induced Hypoxia, Conor Mcauliffe
Seton Hall University Dissertations and Theses (ETDs)
Hypoxia is a frequent characteristic of the solid tumor microenvironment, which occurs when cancer cells lack adequate access to oxygen. By selecting for cells that can adapt to and grow in low oxygen conditions, tumor hypoxia contributes to a more aggressive and invasive cancer phenotype that portends a poor clinical outcome. While many aspects of the cellular response to hypoxia have been explored, the roles of some factors have not been fully explained. Cell signaling factors, including signal transducer and activator of transcription 3 (STAT3), the mu opioid receptor (MOR), and the delta opioid receptor (DOR), as well as changes ...
Epigenetic Regulation Of Crx And Nrl Binding To Regulatory Elements In The Genomes Of Human Retinal Neurons, 2017 James Madison University
Epigenetic Regulation Of Crx And Nrl Binding To Regulatory Elements In The Genomes Of Human Retinal Neurons, Courtney B. Stout
Senior Honors Projects, 2010-current
The retina is a neuronal tissue lining the back of the eye containing rod and cone photoreceptors that make vision possible. Highly regulated transcriptional networks control differentiation and maintenance of photoreceptors in the retina. DNA methylation of cytosine bases in genomic DNA is an epigenetic modification correlated with repression of gene expression. Currently in our lab, the biochemical relationship between DNA methylation and the ability of retina-specific transcription factors to bind in the genome is being studied. These transcription factors, known as cone-rod homeobox (CRX) and neural leucine zipper (NRL), have been shown to act synergistically to control photoreceptor expression ...
Thiol-Ene Chemistry As An Enabler Of New Polymer Structures And Architectures, 2017 University of Massachusetts - Amherst
Thiol-Ene Chemistry As An Enabler Of New Polymer Structures And Architectures, Joel M. Sarapas
This dissertation focuses on two distinct projects: the synthesis and design of novel cell penetrating peptides mimics (CPPMs), and the implementation of the thiol-ene click reaction to generate new polymer architectures and chemistries. Guanidinium-rich CPPMs were generated through both ROMP and RAFT polymerizations, allowing for a comparison to be made across polymer backbone chemistries with respect to both siRNA and protein cellular internalization. A particularly effective methacrylate derived block copolymer was able to deliver siRNA to nearly an entire Jurkat T cell population.
The thiol-ene reaction was implemented initially within the context of improving material design for solid polymer electrolytes ...
Jmh Dissertation 2016.Pdf, 2016 University of Massachusetts - Amherst