Open Access. Powered by Scholars. Published by Universities.®

Developmental Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

909 Full-Text Articles 2051 Authors 75085 Downloads 90 Institutions

All Articles in Developmental Biology

Faceted Search

909 full-text articles. Page 1 of 36.

A Mathematical Model For Selective Differentiation Of Neural Progenitor Cells On Micropatterned Polymer Substrates, Cory L. Howk, Howard A. Levine, Michael W. Smiley, Surya K. Mallapragada, Marit Nilsen-Hamilton, Jisun Oh, Donald S. Sakaguchi 2017 University of Iowa

A Mathematical Model For Selective Differentiation Of Neural Progenitor Cells On Micropatterned Polymer Substrates, Cory L. Howk, Howard A. Levine, Michael W. Smiley, Surya K. Mallapragada, Marit Nilsen-Hamilton, Jisun Oh, Donald S. Sakaguchi

Surya K Mallapragada

The biological hypothesis that the astrocyte-secreted cytokine, interleukin-6 (IL6), stimulates differentiation of adult rat hippocampal progenitor cells (AHPCs) is considered from a mathematical perspective. The proposed mathematical model includes two different mechanisms for stimulation and is based on mass–action kinetics. Both biological mechanisms involve sequential binding, with one pathway solely utilizing surface receptors while the other pathway also involves soluble receptors. Choosing biologically-reasonable values for parameters, simulations of the mathematical model show good agreement with experimental results. A global sensitivity analysis is also conducted to determine both the most influential and non-influential parameters on cellular differentiation, providing additional insights ...


A Mathematical Model For Selective Differentiation Of Neural Progenitor Cells On Micropatterned Polymer Substrates, Cory L. Howk, Howard A. Levine, Michael W. Smiley, Surya K. Mallapragada, Marit Nilsen-Hamilton, Jisun Oh, Donald S. Sakaguchi 2017 University of Iowa

A Mathematical Model For Selective Differentiation Of Neural Progenitor Cells On Micropatterned Polymer Substrates, Cory L. Howk, Howard A. Levine, Michael W. Smiley, Surya K. Mallapragada, Marit Nilsen-Hamilton, Jisun Oh, Donald S. Sakaguchi

Marit Nilsen-Hamilton

The biological hypothesis that the astrocyte-secreted cytokine, interleukin-6 (IL6), stimulates differentiation of adult rat hippocampal progenitor cells (AHPCs) is considered from a mathematical perspective. The proposed mathematical model includes two different mechanisms for stimulation and is based on mass–action kinetics. Both biological mechanisms involve sequential binding, with one pathway solely utilizing surface receptors while the other pathway also involves soluble receptors. Choosing biologically-reasonable values for parameters, simulations of the mathematical model show good agreement with experimental results. A global sensitivity analysis is also conducted to determine both the most influential and non-influential parameters on cellular differentiation, providing additional insights ...


Myo/Nog Cells: Targets For Preventing The Accumulation Of Skeletal Muscle-Like Cells In The Human Lens, Jacquelyn Gerhart, M. Greenbaum, V. Scheinfeld, P. Fitzgerald, M. Crawford, Arturo Bravo Nuevo, M. Pitts, Mindy George-Weinstein 2017 Philadelphia College of Osteopathic Medicine

Myo/Nog Cells: Targets For Preventing The Accumulation Of Skeletal Muscle-Like Cells In The Human Lens, Jacquelyn Gerhart, M. Greenbaum, V. Scheinfeld, P. Fitzgerald, M. Crawford, Arturo Bravo Nuevo, M. Pitts, Mindy George-Weinstein

Arturo Bravo-Nuevo

Posterior capsule opacification (PCO) is a vision impairing condition that arises in some patients following cataract surgery. The fibrotic form of PCO is caused by myofibroblasts that may emerge in the lens years after surgery. In the chick embryo lens, myofibroblasts are derived from Myo/Nog cells that are identified by their expression of the skeletal muscle specific transcription factor MyoD, the bone morphogenetic protein inhibitor Noggin, and the epitope recognized by the G8 monoclonal antibody. The goal of this study was to test the hypothesis that depletion of Myo/Nog cells will prevent the accumulation of myofibroblasts in human ...


Moesin1 And Ve-Cadherin Are Required In Endothelial Cells During In Vivo Tubulogenesis, Ying Wang, Mark S. Kaiser, Jon D. Larson, Aidas Nasevicius, Karl J. Clark, Shannon A. Wadman, Sharon E. Roberg-Perez, Stephen C. Ekker, Perry B. Hackett, Maura McGrail, Jeffrey J. Essner 2017 Iowa State University

Moesin1 And Ve-Cadherin Are Required In Endothelial Cells During In Vivo Tubulogenesis, Ying Wang, Mark S. Kaiser, Jon D. Larson, Aidas Nasevicius, Karl J. Clark, Shannon A. Wadman, Sharon E. Roberg-Perez, Stephen C. Ekker, Perry B. Hackett, Maura Mcgrail, Jeffrey J. Essner

Jeffrey J. Essner

Endothelial tubulogenesis is a crucial step in the formation of functional blood vessels during angiogenesis and vasculogenesis. Here, we use in vivo imaging of living zebrafish embryos expressing fluorescent fusion proteins of β-Actin, α-Catenin, and the ERM family member Moesin1 (Moesin a), to define a novel cord hollowing process that occurs during the initial stages of tubulogenesis in intersegmental vessels (ISVs) in the embryo. We show that the primary lumen elongates along cell junctions between at least two endothelial cells during embryonic angiogenesis. Moesin1-EGFP is enriched around structures that resemble intracellular vacuoles, which fuse with the luminal membrane during expansion ...


Tol2 Gene Trap Integrations In The Zebrafish Amyloid Precursor Protein Genes Appa And Aplp2 Reveal Accumulation Of Secreted App At The Embryonic Veins, Hsin-Kai Liao, Ying Wang, Kristin E. Watt, Qin Wen, Justin Breitback, Chelsy K. Kemmet, Karl J. Clark, Stephen C. Ekker, Jeffrey J. Essner, Maura McGrail 2017 Iowa State University

Tol2 Gene Trap Integrations In The Zebrafish Amyloid Precursor Protein Genes Appa And Aplp2 Reveal Accumulation Of Secreted App At The Embryonic Veins, Hsin-Kai Liao, Ying Wang, Kristin E. Watt, Qin Wen, Justin Breitback, Chelsy K. Kemmet, Karl J. Clark, Stephen C. Ekker, Jeffrey J. Essner, Maura Mcgrail

Jeffrey J. Essner

Background—The single spanning transmembrane amyloid precursor protein (APP) and its proteolytic product, amyloid-beta (Aβ) peptide, have been intensely studied due to their role in the pathogenesis of Alzheimer’s disease. However, the biological role of the secreted ectodomain of APP, which is also generated by proteolytic cleavage, is less well understood. Here, we report Tol2 red fluorescent protein (RFP) transposon gene trap integrations in the zebrafish amyloid precursor protein a (appa) and amyloid precursor-like protein 2 (aplp2) genes. The transposon integrations are predicted to disrupt the appa and aplp2 genes to primarily produce secreted ectodomains of the corresponding proteins ...


Mixed-Surface, Lipid-Tethered Quantum Dots For Targeting Cells And Tissues, Yanjie Zhang, Amanda Haage, Elizabeth M. Whitley, Ian C. Schneider, Aaron R. Clapp 2017 Iowa State University

Mixed-Surface, Lipid-Tethered Quantum Dots For Targeting Cells And Tissues, Yanjie Zhang, Amanda Haage, Elizabeth M. Whitley, Ian C. Schneider, Aaron R. Clapp

Ian C. Schneider

Quantum dots (QDs), with their variable luminescent properties, are rapidly transcending traditional labeling techniques in biological imaging and hold vast potential for biosensing applications. An obstacle in any biosensor development is targeted specificity. Here we report a facile procedure for creating QDs targeted to the cell membrane with the goal of cell-surface protease biosensing. This procedure generates water-soluble QDs with variable coverage of lipid functional groups. The resulting hydrophobicity is quantitatively controlled by the molar ratio of lipids per QD. Appropriate tuning of the hydrophobicity ensures solubility in common aqueous cell culture media and while providing affinity to the lipid ...


Epitaxially Grown Collagen Fibrils Reveal Diversity In Contact Guidance Behavior Among Cancer Cells, Juan Wang, Joseph W. Petefish, Andrew C. Hillier, Ian C. Schneider 2017 Iowa State University

Epitaxially Grown Collagen Fibrils Reveal Diversity In Contact Guidance Behavior Among Cancer Cells, Juan Wang, Joseph W. Petefish, Andrew C. Hillier, Ian C. Schneider

Ian C. Schneider

Invasion of cancer cells into the surrounding tissue is an important step during cancer progression and is driven by cell migration. Cell migration can be random, but often it is directed by various cues such as aligned fibers composed of extracellular matrix (ECM), a process called contact guidance. During contact guidance, aligned fibers bias migration along the long axis of the fibers. These aligned fibers of ECM are commonly composed of type I collagen, an abundant structural protein around tumors. In this paper, we epitaxially grew several different patterns of organized type I collagen on mica and compared the morphology ...


Cellular Contractility And Extracellular Matrix Stiffness Regulate Matrix Metalloproteinase Activity In Pancreatic Cancer Cells, Amanda Haage, Ian C. Schneider 2017 Iowa State University

Cellular Contractility And Extracellular Matrix Stiffness Regulate Matrix Metalloproteinase Activity In Pancreatic Cancer Cells, Amanda Haage, Ian C. Schneider

Ian C. Schneider

The pathogenesis of cancer is often driven by local invasion and metastasis. Recently, mechanical properties of the tumor microenvironment have been identified as potent regulators of invasion and metastasis, while matrix metalloproteinases (MMPs) are classically known as significant enhancers of cancer cell migration and invasion. Here we have been able to sensitively measure MMP activity changes in response to specific extracellular matrix (ECM) environments and cell contractility states. A pancreatic cancer cell line, Panc-1 cells, up-regulate MMP activities between 3- and 10- fold with increased cell contractility. Conversely, they down-regulate MMP activities when contractility is blocked to levels seen with ...


Collagen Attachment To The Substrate Controls Cell Clustering Through Migration, Yue Hou, Laura L. Rodriguez, Juan Wang, Ian C. Schneider 2017 Iowa State University

Collagen Attachment To The Substrate Controls Cell Clustering Through Migration, Yue Hou, Laura L. Rodriguez, Juan Wang, Ian C. Schneider

Ian C. Schneider

Cell clustering and scattering play important roles in cancer progression and tissue engineering. While the extracellular matrix (ECM) is known to control cell clustering, much of the quantitative work has focused on the analysis of clustering between cells with strong cell-cell junctions. Much less is known about how the ECM regulates cells with weak cell-cell contact. Clustering characteristics were quantified in rat adenocarcinoma cells, which form clusters on physically adsorbed collagen substrates, but not on covalently attached collagen substrates. Covalently attaching collagen inhibited desorption of collagen from the surface. While changes in proliferation rate could not explain differences seen in ...


The Number Of Lines A Cell Contacts And Cell Contractility Drive The Efficiency Of Contact Guidance, Nicholas R. Romsey, Yue Hou, Laura L. Rodriguez, Ian C. Schneider 2017 Iowa State University

The Number Of Lines A Cell Contacts And Cell Contractility Drive The Efficiency Of Contact Guidance, Nicholas R. Romsey, Yue Hou, Laura L. Rodriguez, Ian C. Schneider

Ian C. Schneider

Cell migration is an important biological function that impacts many physiological and pathological processes. Often migration is directed along various densities of aligned fibers of collagen, a process called contact guidance. However, cells adhere to other components in the extracellular matrix, possibly affecting migrational behavior. Additionally, changes in intracellular contractility are well known to affect random migration, but its effect on contact guidance is less known. This study examines differences in directed migration in response to variations in the spacing of collagen, non-specific background adhesion strength and myosin II-mediated contractility. Collagen was microcontact printed onto glass substrates and timelapse live-cell ...


Mutations In Tubb8 And Human Oocyte Meiotic Arrest, Ruizhi Feng, Qing Sang, Yanping Kuang, Xiaoxi Sun, Zheng Yan, Shaozhen Zhang, Juanzi Shi, Guoling Tian, Anna Luchniak, Yusuke Fukuda, Bin Li, Min Yu, Junling Chen, Yao Xu, Luo Guo, Ronggui Qu, Xueqian Wang, Zhaogui Sun, Miao Liu, Huijuan Shi, Hongyan Wang, Yi Feng, Ruijin Shao, Renjie Chai, Qiaoli Li, Qinghe Xing, Rui Zhang, Eva Nogales, Li Jin, Lin He, Mohan L. Gupta Jr., Nicholas J. Cowan, Lei Wang 2017 Fudan University

Mutations In Tubb8 And Human Oocyte Meiotic Arrest, Ruizhi Feng, Qing Sang, Yanping Kuang, Xiaoxi Sun, Zheng Yan, Shaozhen Zhang, Juanzi Shi, Guoling Tian, Anna Luchniak, Yusuke Fukuda, Bin Li, Min Yu, Junling Chen, Yao Xu, Luo Guo, Ronggui Qu, Xueqian Wang, Zhaogui Sun, Miao Liu, Huijuan Shi, Hongyan Wang, Yi Feng, Ruijin Shao, Renjie Chai, Qiaoli Li, Qinghe Xing, Rui Zhang, Eva Nogales, Li Jin, Lin He, Mohan L. Gupta Jr., Nicholas J. Cowan, Lei Wang

Mohan Gupta, Jr.

BACKGROUND Human reproduction depends on the fusion of a mature oocyte with a sperm cell to form a fertilized egg. The genetic events that lead to the arrest of human oocyte maturation are unknown.

METHODS We sequenced the exomes of five members of a four-generation family, three of whom had infertility due to oocyte meiosis I arrest. We performed Sanger sequencing of a candidate gene, TUBB8, in DNA samples from these members, additional family members, and members of 23 other affected families. The expression of TUBB8 and all other β-tubulin isotypes was assessed in human oocytes, early embryos, sperm cells ...


Platelet Derived Growth Factor Receptor B (Pdgfrb) Expressing Cells During Zebrafish Coronary Vessel Development, Juancarlos Fierros 2017 California State University, San Bernardino

Platelet Derived Growth Factor Receptor B (Pdgfrb) Expressing Cells During Zebrafish Coronary Vessel Development, Juancarlos Fierros

Electronic Theses, Projects, and Dissertations

Coronary heart disease is a prevalent issue in developed countries throughout the world. It can have crippling effects on the quality of life and even lead to mortality, in the case of myocardial infarction. Part of the problem is the lack of a robust regenerative response in mammals after injury. Zebrafish have an amazing ability to regenerate after injury, and studies have demonstrated that the regenerative response recapitulates embryonic development. Our lab previously reported the first analysis of coronary vessel development in zebrafish and demonstrated that coronary endothelial cells undergo angiogenesis to form a vascular network. The roles of perivascular ...


Epitaxially Grown Collagen Fibrils Reveal Diversity In Contact Guidance Behavior Among Cancer Cells, Juan Wang, Joseph W. Petefish, Andrew C. Hillier, Ian C. Schneider 2017 Iowa State University

Epitaxially Grown Collagen Fibrils Reveal Diversity In Contact Guidance Behavior Among Cancer Cells, Juan Wang, Joseph W. Petefish, Andrew C. Hillier, Ian C. Schneider

Andrew C. Hillier

Invasion of cancer cells into the surrounding tissue is an important step during cancer progression and is driven by cell migration. Cell migration can be random, but often it is directed by various cues such as aligned fibers composed of extracellular matrix (ECM), a process called contact guidance. During contact guidance, aligned fibers bias migration along the long axis of the fibers. These aligned fibers of ECM are commonly composed of type I collagen, an abundant structural protein around tumors. In this paper, we epitaxially grew several different patterns of organized type I collagen on mica and compared the morphology ...


Characterization Of Structure And Function Of The Mouse Retina Using Pattern Electroretinography, Pupil Light Reflex, And Optical Coherence Tomography, Kabhilan Mohan, Matthew M. Harper, Helga Kecova, Eun-Ah Ye, Tatjana Lazic, Donald S. Sakaguchi, Randy H. Kardon, Sinisa D. Grozdanic 2017 Iowa State University

Characterization Of Structure And Function Of The Mouse Retina Using Pattern Electroretinography, Pupil Light Reflex, And Optical Coherence Tomography, Kabhilan Mohan, Matthew M. Harper, Helga Kecova, Eun-Ah Ye, Tatjana Lazic, Donald S. Sakaguchi, Randy H. Kardon, Sinisa D. Grozdanic

Donald Sakaguchi

Objective  To perform in vivo analysis of retinal functional and structural parameters in healthy mouse eyes. Animal Studied  Adult C57BL/6 male mice (n = 37). Procedures  Retinal function was evaluated using pattern electroretinography (pERG) and the chromatic pupil light reflex (cPLR). Structural properties of the retina and nerve fiber layer (NFL) were evaluated using spectral-domain optical coherence tomography (SD-OCT). Results  The average pERG amplitudes were found to be 11.2 ± 0.7 μV (P50-N95, mean ± SEM), with an implicit time for P50-N95 interval of 90.4 ± 5.4 ms. Total retinal thickness was 229.5 ± 1.7 μm (mean ± SEM ...


A Mathematical Model For Selective Differentiation Of Neural Progenitor Cells On Micropatterned Polymer Substrates, Cory L. Howk, Howard A. Levine, Michael W. Smiley, Surya K. Mallapragada, Marit Nilsen-Hamilton, Jisun Oh, Donald S. Sakaguchi 2017 University of Iowa

A Mathematical Model For Selective Differentiation Of Neural Progenitor Cells On Micropatterned Polymer Substrates, Cory L. Howk, Howard A. Levine, Michael W. Smiley, Surya K. Mallapragada, Marit Nilsen-Hamilton, Jisun Oh, Donald S. Sakaguchi

Donald Sakaguchi

The biological hypothesis that the astrocyte-secreted cytokine, interleukin-6 (IL6), stimulates differentiation of adult rat hippocampal progenitor cells (AHPCs) is considered from a mathematical perspective. The proposed mathematical model includes two different mechanisms for stimulation and is based on mass–action kinetics. Both biological mechanisms involve sequential binding, with one pathway solely utilizing surface receptors while the other pathway also involves soluble receptors. Choosing biologically-reasonable values for parameters, simulations of the mathematical model show good agreement with experimental results. A global sensitivity analysis is also conducted to determine both the most influential and non-influential parameters on cellular differentiation, providing additional insights ...


Atomic Force Microscopy And Manipulation Of Living Glial Cells, V. Parpura, P. G. Haydon, D. S. Sakaguchi, E. Henderson 2017 Iowa State University

Atomic Force Microscopy And Manipulation Of Living Glial Cells, V. Parpura, P. G. Haydon, D. S. Sakaguchi, E. Henderson

Donald Sakaguchi

The atomic force microscope (AFM) is capable of imaging surfaces at very high resolution. The AFM has been used to image living glial cells in culture. Typical images reveal the three‐dimensional shape of the cell and often internal cellular structures are visible. In this report, it is shown that by increasing the imaging force, cells can be removed from the surface on which they are grown. Although the forces involved in this process are complex, it is possible to compare relative adhesion of different types of living cells to a particular substrate.


Method And Apparatus For Solid State Molecular Analysis, Eric Henderson, Curtis Mosher, Michael P. Lynch 2017 Iowa State University

Method And Apparatus For Solid State Molecular Analysis, Eric Henderson, Curtis Mosher, Michael P. Lynch

Eric Henderson

The invention is a method for the formation and analysis of novel miniature deposition domains. These deposition domains are placed on a surface to form a molecular array. The molecular array is scanned with an AFM to analyze molecular recognition events and the effect of introduced agents on defined molecular interactions. this approach can be carried out in a high throughput format, allowing rapid screening of thousands of molecular species in a sold state array. The procedures described here have the added benefit of allowing the measurement of changes in the analysis environment or introduction of additional effector molecules to ...


Decontamination Device And Method Thereof, Eric Henderson, James Vesenka 2017 Iowa State University

Decontamination Device And Method Thereof, Eric Henderson, James Vesenka

Eric Henderson

One embodiment of a decontamination device for a surface, such as a probe, and method thereof in accordance with the present invention includes a first electrode, a second electrode, and a transformer. The first electrode comprises a housing with an opening and one end of the second electrode is positioned in the opening. The second electrode has an elongated-shape which tapers to a point at the one end adjacent to the opening. The transformer is mounted in the housing and has first and second pairs of leads. One of the second pair of leads is coupled to the first electrode ...


Device And Method Of Use For Detection And Characterization Of Pathogens And Biological Materials, Eric R. Henderson, Saju R. Nettikadan, Curtis L. Mosher 2017 Iowa State University

Device And Method Of Use For Detection And Characterization Of Pathogens And Biological Materials, Eric R. Henderson, Saju R. Nettikadan, Curtis L. Mosher

Eric Henderson

The present invention includes a method and apparatus for the detection of a target material. The method and apparatus includes providing a substrate with a surface and forming a domains of deposited materials thereon. The deposited material can be placed on the surface and bound directly and non-specifically to the surface, or it may be specifically or non-specifically bound to the surface. The deposited material has an affinity for a specific target material. The domains thus created are termed affinity domains or deposition domains. Multiple affinity domains of deposited materials can be deposited on a single surface, creating a plurality ...


Macrophages Are Necessary For Epimorphic Regeneration In African Spiny Mice, Jennifer Simkin, Thomas R. Gawriluk, John C. Gensel, Ashley W. Seifert 2017 University of Kentucky

Macrophages Are Necessary For Epimorphic Regeneration In African Spiny Mice, Jennifer Simkin, Thomas R. Gawriluk, John C. Gensel, Ashley W. Seifert

Biology Faculty Publications

How the immune system affects tissue regeneration is not well understood. In this study, we used an emerging mammalian model of epimorphic regeneration, the African spiny mouse, to examine cell-based inflammation and tested the hypothesis that macrophages are necessary for regeneration. By directly comparing inflammatory cell activation in a 4 mm ear injury during regeneration (Acomys cahirinus) and scarring (Mus musculus), we found that both species exhibited an acute inflammatory response, with scarring characterized by stronger myeloperoxidase activity. In contrast, ROS production was stronger and more persistent during regeneration. By depleting macrophages during injury, we demonstrate a functional requirement for ...


Digital Commons powered by bepress