Structural Insights Into The Potency Of Sk Channel Positive Modulators, 2017 Chapman University
Structural Insights Into The Potency Of Sk Channel Positive Modulators, Young-Woo Nam, Razan Orfali, Tingting Liu, Kunqian Yu, Meng Cui, Heike Wulff, Miao Zhang
Pharmacy Faculty Articles and Research
Small-conductance Ca2+-activated K+ (SK) channels play essential roles in the regulation of cellular excitability and have been implicated in neurological and cardiovascular diseases through both animal model studies and human genetic association studies. Over the past two decades, positive modulators of SK channels such as NS309 and 1-EBIO have been developed. Our previous structural studies have identified the binding pocket of 1-EBIO and NS309 that is located at the interface between the channel and calmodulin. In this study, we took advantage of four compounds with potencies varying over three orders of magnitude, including 1-EBIO, NS309, SKS-11 (6-bromo-5-methyl-1H-indole-2 ...
Validation Of Minimally-Invasive Sample Collection Methods For Measurement Of Telomere Length, 2017 University of Denver
Validation Of Minimally-Invasive Sample Collection Methods For Measurement Of Telomere Length, Stephanie A. Stout, Jue Lin, Natalie Hernandez, Elyssia Poggi Davis, Judith E. Carroll, Laura M. Glynn
Psychology Faculty Articles and Research
Objective: The discovery of telomere length (TL) as a biomarker of cellular aging and correlate of age-related disease has generated a new field of research in the biology of healthy aging. Although the most common method of sample collection for TL is venous blood draw, less-invasive DNA collection methods are becoming more widely used. However, how TL relates across tissues derived from these sample collection methods is poorly understood. The current study is the first to characterize the associations in TL across three sample collection methods: venous whole blood, finger prick dried blood spot and saliva.
Methods: TL was measured ...
Mechanical Test Methods For Assessing Porcine Carotid And Uterine Artery Burst Pressure Following Ex Vivo Ultrasonic Ligature Seal And Transection, Carissa M. Krane, Margaret Pinnell, Courtney Gardner, Mercedes Thompson, James Coleman, Robert Wilkens
Robert J. Wilkens
A test method was developed to identify those variables important for assessing the performance of ultrasonic surgical devices in ex vivo ligature sealing of porcine carotid and uterine arteries. Ruggedness testing using a small sample size in pilot experiments was conducted using a newly developed test method in an effort to assess the usefulness of this methodology and to identify test variables that might warrant further testing. The development of this test method included the use of a custom-designed prototypic tension device for load-controlled ex vivo vessel stretching during saline perfusion and subsequent seal and transection of porcine arteries with ...
Effects Of Adenovirus Infection On The Localization Of Cellular Protein Pat1b, 2017 Georgia College and State University
Effects Of Adenovirus Infection On The Localization Of Cellular Protein Pat1b, Emilee Friedman, Kasey A. Karen
Georgia Journal of Science
Adenoviruses are a diverse family of nonenveloped, double-stranded DNA viruses with a variety of vertebrate hosts including humans. Over 50 serotypes of human adenovirus have been identified, and cause a number of illnesses, including conjunctivitis, gastroenteritis, and respiratory infections. The life cycle of adenovirus is divided into immediate early, early, and late phases, with immediate early proteins controlling transcription and the cell cycle, early proteins being largely regulatory, and late proteins being structural. Early proteins such as E4 11k have been demonstrated to relocalize key cellular proteins, including proteins found within mRNA processing bodies (p-bodies). It is hypothesized that E4 ...
Detection Of Survival And Proliferation Of Sulfate Reducers Under Simulated Martian Atmospheric And Soil Conditions, 2017 University of Arkansas, Fayetteville
Detection Of Survival And Proliferation Of Sulfate Reducers Under Simulated Martian Atmospheric And Soil Conditions, Sergio Mosquera Mora
Theses and Dissertations
Numerous studies have tried to determine the survivability and proliferation of microorganisms under simulated Martian conditions. Furthermore, most of them have been focused on the ability of these microbes to cope with high brines’ salt (NaCl) concentrations inherent of the Martian surface. However, there are not studies related to the ability of bacteria to survive on subsurface environments that have increasing concentrations of sulfate compounds. For this research, a group of microorganisms known as sulfate-reducing bacteria or simply sulfate reducers were chosen due to their ability to use sulfate compounds as terminal electron acceptors to produce metabolic energy, their tolerance ...
Transcriptome-Based Gene Networks For Systems-Level Analysis Of Plant Gene Functions, 2017 University of Arkansas, Fayetteville
Transcriptome-Based Gene Networks For Systems-Level Analysis Of Plant Gene Functions, Chirag Gupta
Theses and Dissertations
Present day genomic technologies are evolving at an unprecedented rate, allowing interrogation of
cellular activities with increasing breadth and depth. However, we know very little about how the
genome functions and what the identified genes do. The lack of functional annotations of genes
greatly limits the post-analytical interpretation of new high throughput genomic datasets. For plant
biologists, the problem is much severe. Less than 50% of all the identified genes in the model plant
Arabidopsis thaliana, and only about 20% of all genes in the crop model Oryza sativa have some
aspects of their functions assigned. Therefore, there is an ...
Superresolution Imaging Identifies That Conventional Trafficking Pathways Are Not Essential For Endoplasmic Reticulum To Outer Mitochondrial Membrane Protein Transport., 2017 George Washington University
Superresolution Imaging Identifies That Conventional Trafficking Pathways Are Not Essential For Endoplasmic Reticulum To Outer Mitochondrial Membrane Protein Transport., Kyle Salka, Shivaprasad Bhuvanendran, Kassandra Wilson, Petros Bozidis, Mansi Mehta, Kristin Rainey, Hiromi Sesaki, George H Patterson, Jyoti K. Jaiswal, Anamaris M. Colberg-Poley
Genomics and Precision Medicine Faculty Publications
Most nuclear-encoded mitochondrial proteins traffic from the cytosol to mitochondria. Some of these proteins localize at mitochondria-associated membranes (MAM), where mitochondria are closely apposed with the endoplasmic reticulum (ER). We have previously shown that the human cytomegalovirus signal-anchored protein known as viral mitochondria-localized inhibitor of apoptosis (vMIA) traffics from the ER to mitochondria and clusters at the outer mitochondrial membrane (OMM). Here, we have examined the host pathways by which vMIA traffics from the ER to mitochondria and clusters at the OMM. By disruption of phosphofurin acidic cluster sorting protein 2 (PACS-2), mitofusins (Mfn1/2), and dynamin related protein 1 ...
Stochastic Analysis Of A Mammalian Circadian Clock Model: Small Protein Number Effects, 2017 Stanford University
Stochastic Analysis Of A Mammalian Circadian Clock Model: Small Protein Number Effects, David W. Morgens, Blerta Shtylla
Spora: A Journal of Biomathematics
The circadian clock, responsible for coordinating organism function with daily and seasonal changes in the day-night cycle, is controlled by a complex protein network that constitutes a robust biochemical oscillator. Deterministic ordinary differential equation models have been used extensively to model the behavior of these central clocks. However, due to the small number of proteins involved in the circadian oscillations, mathematical models that track stochastic variations in the numbers of clock proteins may reveal more complex and biologically relevant behaviors. In this paper, we compare the response of a robust yet detailed deterministic model for the mammalian circadian clock with ...
Examining The Electrical Excitation, Calcium Signaling, And Mechanical Contraction Cycle In A Heart Cell, Kristen Deetz, Nygel Foster, Darius Leftwich, Chad Meyer, Shalin Patel, Carlos Barajas, Matthias K. Gobbert, Zana Coulibaly
Spora: A Journal of Biomathematics
As the leading cause of death in the United States, heart disease has become a principal concern in modern society. Cardiac arrhythmias can be caused by a dysregulation of calcium dynamics in cardiomyocytes. Calcium dysregulation, however, is not yet fully understood and is not easily predicted; this provides motivation for the subsequent research. Excitation-contraction coupling (ECC) is the process through which cardiomyocytes undergo contraction from an action potential. Calcium induced calcium release (CICR) is the mechanism through which electrical excitation is coupled with mechanical contraction through calcium signaling. The study of the interplay between electrical excitation, calcium signaling, and mechanical ...
About Logan Weihe And Beloved Microcosm, 2017 University of Illinois at Chicago
About Logan Weihe And Beloved Microcosm, Logan M. Weihe
No abstract provided.
Pam-1 Localizations In The Regulation Of Autophagy During Caenorhabditis Elegans Oogenesis, 2017 Murray State University
Pam-1 Localizations In The Regulation Of Autophagy During Caenorhabditis Elegans Oogenesis, Ashley N. Munie
Autophagy, the cell's recycling system, is a highly-conserved survival mechanism of the cell. Autophagy has been implicated in the mediation of the removal of cytotoxic aggregates, such as those linked to neurodegenerative disorders like Huntington and Alzheimer disease. Studies in several model organisms have identified numerous genes involved in mediating autophagy, including the Puromycin sensitive aminopeptidase (Psa). The Caenorhabditis elegans orthologue of Psa, pam-1, also governs fertility. Along with apoptosis, a form of regulated cell death, autophagy has been found to be required for efficient C. elegans oogenesis. When autophagy is suppressed through RNAi in worms harboring a pam-1 ...
Materials And Method For Modifying A Biochemical Component In A Plant, 2017 Iowa State University
Materials And Method For Modifying A Biochemical Component In A Plant, Ling Li, Eve Syrkin Wurtele
A method of modifying the amount of at least one biochemical component in a plant comprising expressing Qua-Quine Starch (QQS) in the plant, the wild-type of which does not express QQS; a transgenic plant, or part thereof, which comprises and expresses QQS as a transgene and in which the amount of at least one biochemical component is modified; a tissue culture of regenerable cells of the transgenic plant; a vector comprising a nucleotide sequence, which encodes the coding sequence of QQS, operably linked to a non-native promoter, which promotes expression of the nucleotide sequence in a plant, which is other ...
Intracellular Signaling And Trafficking In Cancer: Role Of Rab5-Gtpase In Migration And Invasion Of Breast Cells, Nicole Porther
Metastasis is characterized pathologically by uncontrolled cell invasion, proliferation, migration and angiogenesis. Steroid hormones, such as estrogen, and growth factors, which include insulin growth factor I/II (IGF-1/IGF-2) therapy has been associated with most if not all of the features of metastasis. It has been determined that IGF-1 increases cell survival of cancer cells and potentiate the effect of E2 and other ligand growth factors on breast cancer cells. However not much information is available that comprehensively expounds on the roles of insulin growth factor receptor (IGFR) and Rab GTPases may play in breast cancer. The latter, Rab GTPases ...
Investigation Of Moringa Oleifera Leaf Extract And Its Cancer-Selective Antiproliferative Properties, 2017 Southern Wesleyan University
Investigation Of Moringa Oleifera Leaf Extract And Its Cancer-Selective Antiproliferative Properties, Reagen H. Welch, Ashlee H. Tietje
Journal of the South Carolina Academy of Science
Moringa oleifera is a tree native to a number of Asian, African, and Central American countries and has been used in traditional medicine for an assortment of medicinal uses for centuries. Due to bioactive compounds within Moringa leaves, it is believed that Moringa leaf extract may possess cancer-selective antiproliferative properties. Previous research has been conducted in regards to this topic, but poor experimental design due to lack of necessary controls limits the legitimacy of anticancer claims. While previous research has shown that Moringa leaf extract has the potential to kill cancer cells, the research fails to demonstrate the effects of ...
Evaluation Of Gut Modulatory And Bronchodilator Activities Of Amaranthus Spinosus Linn., 2017 Bahauddin Zakariya University
Evaluation Of Gut Modulatory And Bronchodilator Activities Of Amaranthus Spinosus Linn., Mueen Ahmad Chaudhary, Imran Imran, Samra Bashir, Malik Hassan Mehmood, Najeeb -Ur Rehman, Anwar Hassan Gilani
Malik Hassan Mehmood
The aqueous-methanolic extract of Amaranthus spinosus (A. spinosus Linn.,) whole plant, was studied for its laxative, spasmolytic and bronchodilator activities to validate some of its medicinal uses.
The crude extract of A. spinosus was studied in-vivo for bronchodilator and laxative activities and in-vitro using isolated tissue preparations which were mounted in tissue baths assembly containing physiological salt solutions, maintained at 37°C and aerated with carbogen, to assess the spasmolytic effect and to find out the possible underlying mechanisms.
In the in-vivo experiments in mice, the administration of A. spinosus increased fecal output at doses of 100 ...
Gm 20171023m Lsm510meta Convallaria Slide Spectra.Zip, 2017 M.D. Anderson Cancer Center
Gm 20171023m Lsm510meta Convallaria Slide Spectra.Zip, George Mcnamara
Gm Sp8 20171019thu Convallaria Spectra.Lif, 2017 M.D. Anderson Cancer Center
Gm Sp8 20171019thu Convallaria Spectra.Lif, George Mcnamara
Primary Cilium-Dependent Signaling Mechanisms, 2017 Chapman University
Primary Cilium-Dependent Signaling Mechanisms, Rajasekharreddy Pala, Nedaa Alomari, Surya M. Nauli
Pharmacy Faculty Articles and Research
Primary cilia are hair-like organelles and play crucial roles in vertebrate development, organogenesis, health, and many genetic disorders. A primary cilium is a mechano-sensory organelle that responds to mechanical stimuli in the micro-environment. A cilium is also a chemosensor that senses chemical signals surrounding a cell. The overall function of a cilium is therefore to act as a communication hub to transfer extracellular signals into intracellular responses. Although intracellular calcium has been one of the most studied signaling messengers that transmit extracellular signals into the cells, calcium signaling by various ion channels remains a topic of interest in the field ...
Alcohol Consumption Impairs The Ependymal Cilia Motility In The Brain Ventricles, 2017 University of Toledo
Alcohol Consumption Impairs The Ependymal Cilia Motility In The Brain Ventricles, Alzahra J. Al Omran, Hannah C. Saternos, Yusuf S. Althobaiti, Alexander Wisner, Youssef Sari, Surya M. Nauli, Wissam A. Aboualaiwi
Pharmacy Faculty Articles and Research
Ependymal cilia protrude into the central canal of the brain ventricles and spinal cord to circulate the cerebral spinal fluid (CSF). Ependymal cilia dysfunction can hinder the movement of CSF leading to an abnormal accumulation of CSF within the brain known as hydrocephalus. Although the etiology of hydrocephalus was studied before, the effects of ethanol ingestion on ependymal cilia function have not been investigated in vivo. Here, we report three distinct types of ependymal cilia, type-I, type-II and type-III classified based upon their beating frequency, their beating angle, and their distinct localization within the mouse brain-lateral ventricle. Our studies show ...
Modeling Cdc42 Oscillation In Fission Yeast, 2017 Illinois State University
Modeling Cdc42 Oscillation In Fission Yeast, Bin Xu
Annual Symposium on Biomathematics and Ecology: Education and Research
No abstract provided.